Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180001028.01.T01 | XP_003602417.1 | 98.964 | 193 | 2 | 0 | 1 | 193 | 1 | 193 | 8.99E-134 | 382 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180001028.01.T01 | P30707 | 96.373 | 193 | 7 | 0 | 1 | 193 | 1 | 193 | 3.88E-133 | 374 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180001028.01.T01 | G7J9M6 | 98.964 | 193 | 2 | 0 | 1 | 193 | 1 | 193 | 4.29e-134 | 382 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|---|---|
| MsG0180001028.01 | MsG0780039389.01 | PPI |
| MsG0180001028.01 | MsG0380016678.01 | PPI |
| MsG0180001028.01 | MsG0580028107.01 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180001028.01.T01 | MTR_3g093110 | 98.964 | 193 | 2 | 0 | 1 | 193 | 1 | 193 | 1.09e-137 | 382 |
| MsG0180001028.01.T01 | MTR_5g068780 | 86.979 | 192 | 25 | 0 | 1 | 192 | 1 | 192 | 2.86e-125 | 351 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180001028.01.T01 | AT1G33140 | 86.598 | 194 | 25 | 1 | 1 | 193 | 1 | 194 | 1.93e-120 | 339 |
| MsG0180001028.01.T01 | AT1G33120 | 86.598 | 194 | 25 | 1 | 1 | 193 | 1 | 194 | 1.93e-120 | 339 |
| MsG0180001028.01.T01 | AT4G10450 | 84.021 | 194 | 30 | 1 | 1 | 193 | 1 | 194 | 5.65e-119 | 335 |
| MsG0180001028.01.T01 | AT4G10450 | 83.735 | 166 | 26 | 1 | 1 | 165 | 1 | 166 | 4.72e-100 | 286 |
Find 54 sgRNAs with CRISPR-Local
Find 57 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCTTCTTAACATGGCATTTC+TGG | 0.108461 | 1:+14817249 | None:intergenic |
| ATTGAAATCCGAAACTTTCT+TGG | 0.199610 | 1:-14817401 | MsG0180001028.01.T01:CDS |
| TCGATGCTTGGTTTCGTTCC+AGG | 0.312967 | 1:-14817565 | MsG0180001028.01.T01:CDS |
| GGTGAAGGATGAGGTTGTTT+TGG | 0.319250 | 1:-14817318 | MsG0180001028.01.T01:CDS |
| ATGACATTGAGCTTGTTTCT+AGG | 0.326351 | 1:-14817289 | MsG0180001028.01.T01:CDS |
| GTCAGGGATGTTCATGGTTT+CGG | 0.351741 | 1:+14817721 | None:intergenic |
| TTCAATCGACTTGTTGTCAT+TGG | 0.381621 | 1:+14817418 | None:intergenic |
| ACCTTGATGCTAACGCCGTC+AGG | 0.384134 | 1:+14817704 | None:intergenic |
| CTTCTTCTTGCCTTCTTCAT+CGG | 0.396628 | 1:+14817595 | None:intergenic |
| GGAAAACCTCCGCCGCCATC+AGG | 0.416850 | 1:-14817544 | MsG0180001028.01.T01:CDS |
| AGCGGTCCTGATGGCGGCGG+AGG | 0.432147 | 1:+14817538 | None:intergenic |
| GGTATTTATGTTAGTGAGAA+GGG | 0.433044 | 1:-14817203 | MsG0180001028.01.T01:CDS |
| ATTGGTGATGCTAGCGTTGA+TGG | 0.438976 | 1:+14817436 | None:intergenic |
| ATGTTAAGAAGAAGGATATC+AGG | 0.444332 | 1:-14817238 | MsG0180001028.01.T01:CDS |
| GATATCAGGAAGTTTCTTGA+TGG | 0.450730 | 1:-14817224 | MsG0180001028.01.T01:CDS |
| AGGAAAGTAGATTTGCTTGA+TGG | 0.454716 | 1:-14817365 | MsG0180001028.01.T01:CDS |
| AAACTTTCTTGGCGAAAAGA+AGG | 0.483037 | 1:-14817390 | MsG0180001028.01.T01:CDS |
| AACGCCGTCAGGGATGTTCA+TGG | 0.489928 | 1:+14817715 | None:intergenic |
| GATCTCATCACCGATGAAGA+AGG | 0.495068 | 1:-14817605 | MsG0180001028.01.T01:CDS |
| GAAATGCCATGTTAAGAAGA+AGG | 0.499506 | 1:-14817246 | MsG0180001028.01.T01:CDS |
| CCCTGACGGCGTTAGCATCA+AGG | 0.507605 | 1:-14817705 | MsG0180001028.01.T01:CDS |
| TTCAATTGTTCGATCTGAGA+AGG | 0.526562 | 1:-14817339 | MsG0180001028.01.T01:CDS |
| TGGTATTTATGTTAGTGAGA+AGG | 0.531892 | 1:-14817204 | MsG0180001028.01.T01:CDS |
| TGATATCCTTCTTCTTAACA+TGG | 0.532081 | 1:+14817240 | None:intergenic |
| CATGGTTTCGGAGGAGAGGA+TGG | 0.534778 | 1:+14817733 | None:intergenic |
| TTGGTGATGCTAGCGTTGAT+GGG | 0.543746 | 1:+14817437 | None:intergenic |
| TTATGTTAGTGAGAAGGGAT+CGG | 0.552761 | 1:-14817198 | MsG0180001028.01.T01:CDS |
| CATCTTGTAGCGGAAACCCT+TGG | 0.556289 | 1:+14817481 | None:intergenic |
| AGGGTTTCCGCTACAAGATG+AGG | 0.564650 | 1:-14817478 | MsG0180001028.01.T01:CDS |
| ATCTGAGAAGGTGAAGGATG+AGG | 0.567425 | 1:-14817327 | MsG0180001028.01.T01:CDS |
| GTGGCTGAGAGCGGTCCTGA+TGG | 0.569905 | 1:+14817529 | None:intergenic |
| AGAAGCTCAAGATCGATGCT+TGG | 0.573413 | 1:-14817577 | MsG0180001028.01.T01:CDS |
| CTCATCACTGGTGTCACCAA+GGG | 0.576975 | 1:-14817497 | MsG0180001028.01.T01:CDS |
| CACGTCGAGAATCTCATCAC+TGG | 0.583200 | 1:-14817509 | MsG0180001028.01.T01:CDS |
| TGTTCATGGTTTCGGAGGAG+AGG | 0.584113 | 1:+14817729 | None:intergenic |
| TAGCATCAAGGTTAACGCTA+AGG | 0.586158 | 1:-14817693 | MsG0180001028.01.T01:CDS |
| CCTTGATGCTAACGCCGTCA+GGG | 0.590861 | 1:+14817705 | None:intergenic |
| GCTGAGAGCGGTCCTGATGG+CGG | 0.599704 | 1:+14817532 | None:intergenic |
| TCGAAGTCGAAGGACCTCGC+GGG | 0.613305 | 1:-14817667 | MsG0180001028.01.T01:CDS |
| GAGAGCGGTCCTGATGGCGG+CGG | 0.615757 | 1:+14817535 | None:intergenic |
| AGGGATGTTCATGGTTTCGG+AGG | 0.621327 | 1:+14817724 | None:intergenic |
| TCTCATCACTGGTGTCACCA+AGG | 0.623987 | 1:-14817498 | MsG0180001028.01.T01:CDS |
| TGTTCGATCTGAGAAGGTGA+AGG | 0.628133 | 1:-14817333 | MsG0180001028.01.T01:CDS |
| TTCTTGGCGAAAAGAAGGTG+AGG | 0.631315 | 1:-14817385 | MsG0180001028.01.T01:CDS |
| GCTAAGGTCATCGAAGTCGA+AGG | 0.640211 | 1:-14817677 | MsG0180001028.01.T01:CDS |
| ATTCTCGACGTGGCTGAGAG+CGG | 0.641196 | 1:+14817520 | None:intergenic |
| TGTTAGTGAGAAGGGATCGG+TGG | 0.651106 | 1:-14817195 | MsG0180001028.01.T01:CDS |
| ATCGAAGTCGAAGGACCTCG+CGG | 0.660265 | 1:-14817668 | MsG0180001028.01.T01:CDS |
| TGGTGATGCTAGCGTTGATG+GGG | 0.676063 | 1:+14817438 | None:intergenic |
| CGGTGATGAGATCAAAATCG+AGG | 0.679345 | 1:+14817615 | None:intergenic |
| CAGTGATGAGATTCTCGACG+TGG | 0.688369 | 1:+14817510 | None:intergenic |
| ACACAAACCTCATCTTGTAG+CGG | 0.691692 | 1:+14817471 | None:intergenic |
| GAAACCATGAACATCCCTGA+CGG | 0.701649 | 1:-14817719 | MsG0180001028.01.T01:CDS |
| TCACGAACAAGCTTCCCGCG+AGG | 0.748381 | 1:+14817653 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ATGTTAAGAAGAAGGATATC+AGG | - | Chr1:14817683-14817702 | MsG0180001028.01.T01:CDS | 30.0% | |
| ATTGAAATCCGAAACTTTCT+TGG | - | Chr1:14817520-14817539 | MsG0180001028.01.T01:CDS | 30.0% | |
| GGTATTTATGTTAGTGAGAA+GGG | - | Chr1:14817718-14817737 | MsG0180001028.01.T01:CDS | 30.0% | |
| TGATATCCTTCTTCTTAACA+TGG | + | Chr1:14817684-14817703 | None:intergenic | 30.0% | |
| TGGTATTTATGTTAGTGAGA+AGG | - | Chr1:14817717-14817736 | MsG0180001028.01.T01:CDS | 30.0% | |
| AAACTTTCTTGGCGAAAAGA+AGG | - | Chr1:14817531-14817550 | MsG0180001028.01.T01:CDS | 35.0% | |
| GAAATGCCATGTTAAGAAGA+AGG | - | Chr1:14817675-14817694 | MsG0180001028.01.T01:CDS | 35.0% | |
| GATATCAGGAAGTTTCTTGA+TGG | - | Chr1:14817697-14817716 | MsG0180001028.01.T01:CDS | 35.0% | |
| TCTTCTTAACATGGCATTTC+TGG | + | Chr1:14817675-14817694 | None:intergenic | 35.0% | |
| TTATGTTAGTGAGAAGGGAT+CGG | - | Chr1:14817723-14817742 | MsG0180001028.01.T01:CDS | 35.0% | |
| TTCAATTGTTCGATCTGAGA+AGG | - | Chr1:14817582-14817601 | MsG0180001028.01.T01:CDS | 35.0% | |
| ! | AGGAAAGTAGATTTGCTTGA+TGG | - | Chr1:14817556-14817575 | MsG0180001028.01.T01:CDS | 35.0% |
| ! | ATGACATTGAGCTTGTTTCT+AGG | - | Chr1:14817632-14817651 | MsG0180001028.01.T01:CDS | 35.0% |
| ! | TCTTTTCGCCAAGAAAGTTT+CGG | + | Chr1:14817531-14817550 | None:intergenic | 35.0% |
| !! | TTCAATCGACTTGTTGTCAT+TGG | + | Chr1:14817506-14817525 | None:intergenic | 35.0% |
| ACACAAACCTCATCTTGTAG+CGG | + | Chr1:14817453-14817472 | None:intergenic | 40.0% | |
| CTTCTTCTTGCCTTCTTCAT+CGG | + | Chr1:14817329-14817348 | None:intergenic | 40.0% | |
| TAGCATCAAGGTTAACGCTA+AGG | - | Chr1:14817228-14817247 | MsG0180001028.01.T01:CDS | 40.0% | |
| !!! | AAGGATGAGGTTGTTTTGGA+TGG | - | Chr1:14817607-14817626 | MsG0180001028.01.T01:CDS | 40.0% |
| AGAAGCTCAAGATCGATGCT+TGG | - | Chr1:14817344-14817363 | MsG0180001028.01.T01:CDS | 45.0% | |
| ATCTGAGAAGGTGAAGGATG+AGG | - | Chr1:14817594-14817613 | MsG0180001028.01.T01:CDS | 45.0% | |
| CGGTGATGAGATCAAAATCG+AGG | + | Chr1:14817309-14817328 | None:intergenic | 45.0% | |
| GAAACCATGAACATCCCTGA+CGG | - | Chr1:14817202-14817221 | MsG0180001028.01.T01:CDS | 45.0% | |
| GATCTCATCACCGATGAAGA+AGG | - | Chr1:14817316-14817335 | MsG0180001028.01.T01:CDS | 45.0% | |
| GTCAGGGATGTTCATGGTTT+CGG | + | Chr1:14817203-14817222 | None:intergenic | 45.0% | |
| TGTTCGATCTGAGAAGGTGA+AGG | - | Chr1:14817588-14817607 | MsG0180001028.01.T01:CDS | 45.0% | |
| TTCTTGGCGAAAAGAAGGTG+AGG | - | Chr1:14817536-14817555 | MsG0180001028.01.T01:CDS | 45.0% | |
| ! | GGTGAAGGATGAGGTTGTTT+TGG | - | Chr1:14817603-14817622 | MsG0180001028.01.T01:CDS | 45.0% |
| !! | ATTGGTGATGCTAGCGTTGA+TGG | + | Chr1:14817488-14817507 | None:intergenic | 45.0% |
| !! | TTGGTGATGCTAGCGTTGAT+GGG | + | Chr1:14817487-14817506 | None:intergenic | 45.0% |
| AGGGATGTTCATGGTTTCGG+AGG | + | Chr1:14817200-14817219 | None:intergenic | 50.0% | |
| AGGGTTTCCGCTACAAGATG+AGG | - | Chr1:14817443-14817462 | MsG0180001028.01.T01:CDS | 50.0% | |
| CACGTCGAGAATCTCATCAC+TGG | - | Chr1:14817412-14817431 | MsG0180001028.01.T01:CDS | 50.0% | |
| CAGTGATGAGATTCTCGACG+TGG | + | Chr1:14817414-14817433 | None:intergenic | 50.0% | |
| GCTAAGGTCATCGAAGTCGA+AGG | - | Chr1:14817244-14817263 | MsG0180001028.01.T01:CDS | 50.0% | |
| TCGATGCTTGGTTTCGTTCC+AGG | - | Chr1:14817356-14817375 | MsG0180001028.01.T01:CDS | 50.0% | |
| TGTTAGTGAGAAGGGATCGG+TGG | - | Chr1:14817726-14817745 | MsG0180001028.01.T01:CDS | 50.0% | |
| TGTTCATGGTTTCGGAGGAG+AGG | + | Chr1:14817195-14817214 | None:intergenic | 50.0% | |
| ! | CATCTTGTAGCGGAAACCCT+TGG | + | Chr1:14817443-14817462 | None:intergenic | 50.0% |
| !! | CTCATCACTGGTGTCACCAA+GGG | - | Chr1:14817424-14817443 | MsG0180001028.01.T01:CDS | 50.0% |
| !! | TCTCATCACTGGTGTCACCA+AGG | - | Chr1:14817423-14817442 | MsG0180001028.01.T01:CDS | 50.0% |
| !! | TGGTGATGCTAGCGTTGATG+GGG | + | Chr1:14817486-14817505 | None:intergenic | 50.0% |
| AACGCCGTCAGGGATGTTCA+TGG | + | Chr1:14817209-14817228 | None:intergenic | 55.0% | |
| ACCTTGATGCTAACGCCGTC+AGG | + | Chr1:14817220-14817239 | None:intergenic | 55.0% | |
| ATCGAAGTCGAAGGACCTCG+CGG | - | Chr1:14817253-14817272 | MsG0180001028.01.T01:CDS | 55.0% | |
| ATTCTCGACGTGGCTGAGAG+CGG | + | Chr1:14817404-14817423 | None:intergenic | 55.0% | |
| CATGGTTTCGGAGGAGAGGA+TGG | + | Chr1:14817191-14817210 | None:intergenic | 55.0% | |
| CCTTGATGCTAACGCCGTCA+GGG | + | Chr1:14817219-14817238 | None:intergenic | 55.0% | |
| CCCTGACGGCGTTAGCATCA+AGG | - | Chr1:14817216-14817235 | MsG0180001028.01.T01:CDS | 60.0% | |
| TCACGAACAAGCTTCCCGCG+AGG | + | Chr1:14817271-14817290 | None:intergenic | 60.0% | |
| TCGAAGTCGAAGGACCTCGC+GGG | - | Chr1:14817254-14817273 | MsG0180001028.01.T01:CDS | 60.0% | |
| GCTGAGAGCGGTCCTGATGG+CGG | + | Chr1:14817392-14817411 | None:intergenic | 65.0% | |
| GGAAAACCTCCGCCGCCATC+AGG | - | Chr1:14817377-14817396 | MsG0180001028.01.T01:CDS | 65.0% | |
| GTGGCTGAGAGCGGTCCTGA+TGG | + | Chr1:14817395-14817414 | None:intergenic | 65.0% | |
| !! | GATGGCGGCGGAGGTTTTCC+TGG | + | Chr1:14817377-14817396 | None:intergenic | 65.0% |
| !! | GAGAGCGGTCCTGATGGCGG+CGG | + | Chr1:14817389-14817408 | None:intergenic | 70.0% |
| !! | AGCGGTCCTGATGGCGGCGG+AGG | + | Chr1:14817386-14817405 | None:intergenic | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr1 | gene | 14817181 | 14817762 | 14817181 | ID=MsG0180001028.01;Name=MsG0180001028.01 |
| Chr1 | mRNA | 14817181 | 14817762 | 14817181 | ID=MsG0180001028.01.T01;Parent=MsG0180001028.01;Name=MsG0180001028.01.T01;_AED=0.49;_eAED=0.49;_QI=0|-1|0|1|-1|1|1|0|193 |
| Chr1 | exon | 14817181 | 14817762 | 14817181 | ID=MsG0180001028.01.T01:exon:30865;Parent=MsG0180001028.01.T01 |
| Chr1 | CDS | 14817181 | 14817762 | 14817181 | ID=MsG0180001028.01.T01:cds;Parent=MsG0180001028.01.T01 |
| Gene Sequence |
| Protein sequence |
