AlfalfaGEDB | MsG0380016678.01

M. sativa cultivar ZhongmuNo.1 / MsG0380016678.01

KEGG: K02940
GO: GO:0003735 GO:0005622 GO:0005840 GO:0006412 GO:0019843
PFAM: PF00347

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380016678.01.T01	XP_003602417.1	99.482	193	1	0	1	193	1	193	6.32E-135	385

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380016678.01.T01	P30707	96.891	193	6	0	1	193	1	193	1.54E-134	377

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380016678.01.T01	G7J9M6	99.482	193	1	0	1	193	1	193	3.02e-135	385

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080048040.01	MsG0380016678.01	0.840091	1.071622e-57	1.510280e-54
MsG0080048922.01	MsG0380016678.01	0.882926	6.913221e-71	4.292641e-67
MsG0080048933.01	MsG0380016678.01	0.802018	6.844225e-49	3.316970e-46
MsG0180000096.01	MsG0380016678.01	0.819453	1.152245e-52	8.870765e-50
MsG0180000118.01	MsG0380016678.01	0.874025	9.317899e-68	4.130223e-64
MsG0180001169.01	MsG0380016678.01	0.820699	5.975973e-53	4.762627e-50
MsG0180001829.01	MsG0380016678.01	0.824350	8.466277e-54	7.479682e-51
MsG0180002738.01	MsG0380016678.01	0.884951	1.235911e-71	8.322338e-68
MsG0180003313.01	MsG0380016678.01	0.841587	4.341681e-58	6.409277e-55
MsG0180003585.01	MsG0380016678.01	0.866466	2.793276e-65	9.423486e-62
MsG0180003897.01	MsG0380016678.01	0.886279	3.928541e-72	2.787964e-68
MsG0180004503.01	MsG0380016678.01	0.806600	7.602148e-50	4.143297e-47
MsG0180004609.01	MsG0380016678.01	0.809807	1.576524e-50	9.347365e-48
MsG0180004829.01	MsG0380016678.01	0.882632	8.848592e-71	5.428382e-67
MsG0180004946.01	MsG0380016678.01	0.835442	1.674217e-56	2.047541e-53
MsG0180005384.01	MsG0380016678.01	0.878882	1.959798e-69	1.040196e-65
MsG0180005573.01	MsG0380016678.01	0.940865	1.121823e-100	1.260156e-95
MsG0280006301.01	MsG0380016678.01	0.862650	4.361067e-64	1.286554e-60
MsG0280010995.01	MsG0380016678.01	0.844895	5.692842e-59	9.323710e-56
MsG0280011060.01	MsG0380016678.01	0.843955	1.019321e-58	1.620927e-55
MsG0280011308.01	MsG0380016678.01	0.823976	1.036437e-53	9.060857e-51
MsG0380011643.01	MsG0380016678.01	0.826065	3.327642e-54	3.087684e-51
MsG0380014507.01	MsG0380016678.01	0.811269	7.617562e-51	4.694235e-48
MsG0380015494.01	MsG0380016678.01	0.922329	1.132400e-88	4.255826e-84
MsG0380015857.01	MsG0380016678.01	0.876719	1.116210e-68	5.467945e-65
MsG0380016607.01	MsG0380016678.01	0.801479	8.829960e-49	4.221280e-46
MsG0380016622.01	MsG0380016678.01	0.823972	1.038555e-53	9.078328e-51
MsG0380016678.01	MsG0380016744.01	0.853649	2.078882e-61	4.523253e-58
MsG0380016678.01	MsG0380016760.01	0.864610	1.074608e-64	3.394727e-61
MsG0380016678.01	MsG0380017496.01	0.802283	6.038319e-49	2.946311e-46
MsG0380016678.01	MsG0380018056.01	0.859969	2.863455e-63	7.701961e-60
MsG0380016678.01	MsG0480018164.01	0.879753	9.629674e-70	5.284265e-66
MsG0380016678.01	MsG0480019165.01	0.894577	2.163332e-75	2.163165e-71
MsG0380016678.01	MsG0480019632.01	0.912902	1.142742e-83	2.630773e-79
MsG0380016678.01	MsG0480020796.01	0.875700	2.505060e-68	1.181332e-64
MsG0380016678.01	MsG0480020895.01	0.869208	3.678173e-66	1.368927e-62
MsG0380016678.01	MsG0480020911.01	0.931060	6.594171e-94	4.073881e-89
MsG0380016678.01	MsG0480021279.01	0.909279	6.790377e-82	1.311258e-77
MsG0380016678.01	MsG0480021417.01	0.930722	1.081594e-93	6.531911e-89
MsG0380016678.01	MsG0480022006.01	0.855650	5.472190e-62	1.272319e-58
MsG0380016678.01	MsG0480022041.01	0.828687	7.825521e-55	7.834700e-52
MsG0380016678.01	MsG0480022363.01	0.955848	1.172802e-113	3.662393e-108
MsG0380016678.01	MsG0480022789.01	0.838516	2.745132e-57	3.685610e-54
MsG0380016678.01	MsG0480022955.01	0.837206	5.958294e-57	7.684290e-54
MsG0380016678.01	MsG0480023050.01	0.821060	4.934577e-53	3.972975e-50
MsG0380016678.01	MsG0480023380.01	0.916004	2.996721e-85	8.063858e-81
MsG0380016678.01	MsG0480023770.01	0.807722	4.398389e-50	2.468058e-47
MsG0380016678.01	MsG0480023774.01	0.895196	1.206239e-75	1.238517e-71
MsG0380016678.01	MsG0480023931.01	0.862163	6.156781e-64	1.785888e-60
MsG0380016678.01	MsG0480023970.01	0.824405	8.215989e-54	7.269900e-51
MsG0380016678.01	MsG0480023996.01	0.868219	7.680706e-66	2.756733e-62
MsG0380016678.01	MsG0580024059.01	0.808421	3.122300e-50	1.784514e-47
MsG0380016678.01	MsG0580024481.01	0.859640	3.596791e-63	9.565266e-60
MsG0380016678.01	MsG0580024540.01	0.879224	1.483189e-69	7.977790e-66
MsG0380016678.01	MsG0580024738.01	0.912401	2.031994e-83	4.564065e-79
MsG0380016678.01	MsG0580024894.01	0.895683	7.592010e-76	7.957658e-72
MsG0380016678.01	MsG0580025073.01	0.856431	3.233009e-62	7.715544e-59
MsG0380016678.01	MsG0580025348.01	0.835720	1.423598e-56	1.755676e-53
MsG0380016678.01	MsG0580025750.01	0.924114	1.084708e-89	4.504670e-85
MsG0380016678.01	MsG0580026749.01	0.800630	1.316798e-48	6.161395e-46
MsG0380016678.01	MsG0580029047.01	0.808282	3.344077e-50	1.904300e-47
MsG0380016678.01	MsG0580029284.01	0.895128	1.286162e-75	1.317018e-71
MsG0380016678.01	MsG0580029498.01	0.812835	3.471796e-51	2.230414e-48
MsG0380016678.01	MsG0580029702.01	0.876039	1.916140e-68	9.151580e-65
MsG0380016678.01	MsG0580029703.01	0.831713	1.428448e-55	1.562998e-52
MsG0380016678.01	MsG0580030073.01	0.907539	4.541197e-81	8.090226e-77
MsG0380016678.01	MsG0680031368.01	0.886979	2.133806e-72	1.557779e-68
MsG0380016678.01	MsG0680031763.01	0.839912	1.193087e-57	1.671958e-54
MsG0380016678.01	MsG0680032459.01	0.879309	1.384623e-69	7.471026e-66
MsG0380016678.01	MsG0680035611.01	0.891222	4.837146e-74	4.201923e-70
MsG0380016678.01	MsG0780036015.01	0.820884	5.417994e-53	4.340527e-50
MsG0380016678.01	MsG0780036859.01	0.835449	1.666869e-56	2.038998e-53
MsG0380016678.01	MsG0780037097.01	0.880942	3.620317e-70	2.080571e-66
MsG0380016678.01	MsG0780037290.01	0.807651	4.555575e-50	2.551371e-47
MsG0380016678.01	MsG0780038383.01	0.910852	1.177806e-82	2.453365e-78
MsG0380016678.01	MsG0780041058.01	0.927317	1.391359e-91	6.897717e-87
MsG0380016678.01	MsG0780041240.01	0.840444	8.661562e-58	1.234032e-54
MsG0380016678.01	MsG0780041244.01	0.864130	1.517348e-64	4.712337e-61
MsG0380016678.01	MsG0780041332.01	0.823249	1.533283e-53	1.313106e-50
MsG0380016678.01	MsG0780041461.01	0.924847	4.072831e-90	1.757983e-85
MsG0380016678.01	MsG0780041522.01	0.944513	1.697410e-103	2.441180e-98
MsG0380016678.01	MsG0780041790.01	0.842279	2.848498e-58	4.297394e-55
MsG0380016678.01	MsG0880041941.01	0.835594	1.532039e-56	1.882138e-53
MsG0380016678.01	MsG0880042209.01	0.813736	2.200489e-51	1.448703e-48
MsG0380016678.01	MsG0880042517.01	0.927892	6.226471e-92	3.186005e-87
MsG0380016678.01	MsG0880043076.01	0.934334	4.740078e-96	3.559915e-91
MsG0380016678.01	MsG0880043517.01	0.856087	4.077658e-62	9.619383e-59
MsG0380016678.01	MsG0880044003.01	0.838948	2.122437e-57	2.887254e-54
MsG0380016678.01	MsG0880044204.01	0.825645	4.187349e-54	3.839283e-51
MsG0380016678.01	MsG0880045836.01	0.862481	4.916717e-64	1.442189e-60
MsG0380016678.01	MsG0880046220.01	0.891877	2.658791e-74	2.373006e-70
MsG0380016678.01	MsG0880046760.01	0.852093	5.786586e-61	1.195977e-57
MsG0380016678.01	MsG0880047310.01	0.868859	4.772706e-66	1.753307e-62
MsG0280006629.01	MsG0380016678.01	0.896513	3.432832e-76	3.726402e-72
MsG0280006747.01	MsG0380016678.01	0.881177	2.981936e-70	1.729296e-66
MsG0280007055.01	MsG0380016678.01	0.914240	2.417296e-84	5.947680e-80
MsG0280007643.01	MsG0380016678.01	0.819342	1.221263e-52	9.371909e-50
MsG0280007995.01	MsG0380016678.01	0.859295	4.566693e-63	1.200049e-59
MsG0280009372.01	MsG0380016678.01	0.829221	5.810105e-55	5.909254e-52
MsG0280010232.01	MsG0380016678.01	0.833201	6.108880e-56	6.985617e-53
MsG0280010495.01	MsG0380016678.01	0.804700	1.904924e-49	9.888351e-47
MsG0280010519.01	MsG0380016678.01	0.872387	3.307818e-67	1.381599e-63

PPI

Gene1	Gene2	Type
MsG0180001028.01	MsG0380016678.01	PPI
MsG0380016678.01	MsG0580028107.01	PPI
MsG0780039389.01	MsG0380016678.01	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380016678.01.T01	MTR_3g093110	99.482	193	1	0	1	193	1	193	7.65e-139	385
MsG0380016678.01.T01	MTR_5g068780	87.500	192	24	0	1	192	1	192	1.42e-126	354

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0380016678.01.T01	AT1G33140	87.113	194	24	1	1	193	1	194	9.77e-122	342
MsG0380016678.01.T01	AT1G33120	87.113	194	24	1	1	193	1	194	9.77e-122	342
MsG0380016678.01.T01	AT4G10450	84.536	194	29	1	1	193	1	194	2.62e-120	338
MsG0380016678.01.T01	AT4G10450	84.337	166	25	1	1	165	1	166	2.47e-101	290

Find 64 sgRNAs with CRISPR-Local

Find 91 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AGCTCAGTTCGTTAAATTAT+TGG	0.181439	3:+86500792	MsG0380016678.01.T01:three_prime_UTR
ATTGAGATCCGAAACTTTCT+TGG	0.238218	3:+86499882	MsG0380016678.01.T01:CDS
TTACATTTGCTTGTTGAGTT+TGG	0.269604	3:+86500673	MsG0380016678.01.T01:three_prime_UTR
GGTGAAGGATGAGGTTGTTT+TGG	0.319250	3:+86500318	MsG0380016678.01.T01:CDS
ATGACATTGAGCTTGTTTCT+AGG	0.326351	3:+86500347	MsG0380016678.01.T01:CDS
ATTCAAGTTTCTGTTGGTCT+TGG	0.332651	3:+86500613	MsG0380016678.01.T01:three_prime_UTR
TCGATGCCTGGTTCGGTTCC+AGG	0.334604	3:+86499718	MsG0380016678.01.T01:CDS
GTCAGGGATGTTCATGGTTT+CGG	0.351741	3:-86499562	None:intergenic
TCTTCTCTCCAAGAAAGTTT+CGG	0.365793	3:-86499890	None:intergenic
TTCTATATGTTTGAAATGAT+AGG	0.374982	3:+86500978	MsG0380016678.01.T01:three_prime_UTR
ACCTTGATGCTAACGCCGTC+AGG	0.384134	3:-86499579	None:intergenic
CTTCTTCTTGCCTTCTTCAT+CGG	0.396628	3:-86499688	None:intergenic
CTCAATCGACTTGTTGTCAT+TGG	0.397158	3:-86499865	None:intergenic
GGTATTTATGTTAGTGAGAA+GGG	0.433044	3:+86500529	MsG0380016678.01.T01:CDS
AGTTTCTGTTGGTCTTGGAT+TGG	0.433152	3:+86500618	MsG0380016678.01.T01:three_prime_UTR
ATTGGTGATGCTAGCGTTGA+TGG	0.438976	3:-86499847	None:intergenic
ATGTTAAGAAGAAGGATATC+AGG	0.444332	3:+86500494	MsG0380016678.01.T01:CDS
CTCAAGATCGATGCCTGGTT+CGG	0.447724	3:+86499711	MsG0380016678.01.T01:CDS
GATATCAGGAAGTTTCTTGA+TGG	0.450730	3:+86500508	MsG0380016678.01.T01:CDS
AGGAAAGTAGATTTGCTTGA+TGG	0.454716	3:+86500271	MsG0380016678.01.T01:CDS
AGTTTAATTCAAGTTTCTGT+TGG	0.456312	3:+86500607	MsG0380016678.01.T01:three_prime_UTR
AGCAGTTCTGATGGCGGCGG+AGG	0.457127	3:-86499745	None:intergenic
TCTGTGTTTGCAGCACTCTC+CGG	0.465365	3:-86499481	None:intergenic
TGGTTAAATAATGCACTGAA+TGG	0.480150	3:+86500867	MsG0380016678.01.T01:three_prime_UTR
GGTGGTAACTGAAGAATAGA+TGG	0.489244	3:+86500555	MsG0380016678.01.T01:exon
AACGCCGTCAGGGATGTTCA+TGG	0.489928	3:-86499568	None:intergenic
GAAAGCTCAGAGTATGAGTC+TGG	0.491000	3:+86501000	MsG0380016678.01.T01:three_prime_UTR
AAACTTTCTTGGAGAGAAGA+AGG	0.493598	3:+86499893	MsG0380016678.01.T01:CDS
GATCTCATCACCGATGAAGA+AGG	0.495068	3:+86499678	MsG0380016678.01.T01:CDS
GAAATGCCATGTTAAGAAGA+AGG	0.499506	3:+86500486	MsG0380016678.01.T01:intron
CCCTGACGGCGTTAGCATCA+AGG	0.507605	3:+86499578	MsG0380016678.01.T01:CDS
TTCAATTGTTCGATCTGAGA+AGG	0.526562	3:+86500297	MsG0380016678.01.T01:CDS
TGGAAATCTCACACACTCTA+CGG	0.531126	3:-86499508	None:intergenic
TGGTATTTATGTTAGTGAGA+AGG	0.531892	3:+86500528	MsG0380016678.01.T01:CDS
TGATATCCTTCTTCTTAACA+TGG	0.532081	3:-86500492	None:intergenic
CATGGTTTCGGAGGAGAGGA+TGG	0.534778	3:-86499550	None:intergenic
TTGGTGATGCTAGCGTTGAT+GGG	0.543746	3:-86499846	None:intergenic
TTATGTTAGTGAGAAGGGAT+CGG	0.552761	3:+86500534	MsG0380016678.01.T01:CDS
CACGTTGAGAATCTCATCAC+TGG	0.554104	3:+86499774	MsG0380016678.01.T01:CDS
CATCTTGTAGCGGAAACCCT+TGG	0.556289	3:-86499802	None:intergenic
AGGGTTTCCGCTACAAGATG+AGG	0.564650	3:+86499805	MsG0380016678.01.T01:CDS
ATCTGAGAAGGTGAAGGATG+AGG	0.567425	3:+86500309	MsG0380016678.01.T01:CDS
GAGAGCAGTTCTGATGGCGG+CGG	0.574544	3:-86499748	None:intergenic
CTCATCACTGGTGTCACCAA+GGG	0.576975	3:+86499786	MsG0380016678.01.T01:CDS
GTGGCTGAGAGCAGTTCTGA+TGG	0.581831	3:-86499754	None:intergenic
TGTTCATGGTTTCGGAGGAG+AGG	0.584113	3:-86499554	None:intergenic
TAGCATCAAGGTTAACGCTA+AGG	0.586158	3:+86499590	MsG0380016678.01.T01:CDS
AGAAGCTCAAGATCGATGCC+TGG	0.588777	3:+86499706	MsG0380016678.01.T01:CDS
CCTTGATGCTAACGCCGTCA+GGG	0.590861	3:-86499578	None:intergenic
TCGAAGTCGAAGGACCTCGC+GGG	0.613305	3:+86499616	MsG0380016678.01.T01:CDS
AGGGATGTTCATGGTTTCGG+AGG	0.621327	3:-86499559	None:intergenic
TCTCATCACTGGTGTCACCA+AGG	0.623987	3:+86499785	MsG0380016678.01.T01:CDS
GTCATGTGCACTTATTAACC+AGG	0.625933	3:+86500369	MsG0380016678.01.T01:CDS
TGTTCGATCTGAGAAGGTGA+AGG	0.628133	3:+86500303	MsG0380016678.01.T01:CDS
GCTAAGGTCATCGAAGTCGA+AGG	0.640211	3:+86499606	MsG0380016678.01.T01:CDS
TGTTAGTGAGAAGGGATCGG+TGG	0.651106	3:+86500537	MsG0380016678.01.T01:CDS
ATCGAAGTCGAAGGACCTCG+CGG	0.660265	3:+86499615	MsG0380016678.01.T01:CDS
TGGTGATGCTAGCGTTGATG+GGG	0.676063	3:-86499845	None:intergenic
GCTGAGAGCAGTTCTGATGG+CGG	0.677632	3:-86499751	None:intergenic
CGGTGATGAGATCAAAATCG+AGG	0.680504	3:-86499668	None:intergenic
AGACAAACCTCATCTTGTAG+CGG	0.682924	3:-86499812	None:intergenic
GAAACCATGAACATCCCTGA+CGG	0.701649	3:+86499564	MsG0380016678.01.T01:CDS
CAGTGATGAGATTCTCAACG+TGG	0.725921	3:-86499773	None:intergenic
TCACGAACAAGCTTCCCGCG+AGG	0.748381	3:-86499630	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TATAAATTAGATTAAAACTA+GGG	-	Chr3:86500031-86500050	None:intergenic	10.0%
!!	TTATAAATTAGATTAAAACT+AGG	-	Chr3:86500032-86500051	None:intergenic	10.0%
!!!	ATGAGTTAATTTTTTTATTT+TGG	+	Chr3:86500139-86500158	MsG0380016678.01.T01:intron	10.0%
!!!	TATTGTTCTTATATGTTTTA+AGG	+	Chr3:86500741-86500760	MsG0380016678.01.T01:three_prime_UTR	15.0%
!!!	TCTTTAATTTCTTCAATTTT+AGG	+	Chr3:86499948-86499967	MsG0380016678.01.T01:intron	15.0%
!!	GAGATAATGTCTAATTTATT+TGG	+	Chr3:86500173-86500192	MsG0380016678.01.T01:intron	20.0%
!!!	TTCTATATGTTTGAAATGAT+AGG	+	Chr3:86500978-86500997	MsG0380016678.01.T01:three_prime_UTR	20.0%
!!!	TTTGGAATTTTTGGTTATTT+TGG	+	Chr3:86500691-86500710	MsG0380016678.01.T01:three_prime_UTR	20.0%
!	ACAAAAATAAAGCATGTTCA+TGG	-	Chr3:86500413-86500432	None:intergenic	25.0%
!	CACAAATTAAAGTGAAACTA+AGG	-	Chr3:86499982-86500001	None:intergenic	25.0%
!	GAATCGAATAATGTTGTATT+AGG	+	Chr3:86500246-86500265	MsG0380016678.01.T01:intron	25.0%
!	GTCTAATTTATTTGGTTATC+TGG	+	Chr3:86500181-86500200	MsG0380016678.01.T01:intron	25.0%
!!	AGTTTAATTCAAGTTTCTGT+TGG	+	Chr3:86500607-86500626	MsG0380016678.01.T01:three_prime_UTR	25.0%
!!	GTTTTCATTGAATTATTCAG+TGG	+	Chr3:86500847-86500866	MsG0380016678.01.T01:three_prime_UTR	25.0%
!!!	GTTATTTTGGTTTTCTCTAT+TGG	+	Chr3:86500704-86500723	MsG0380016678.01.T01:three_prime_UTR	25.0%
	AGCTCAGTTCGTTAAATTAT+TGG	+	Chr3:86500792-86500811	MsG0380016678.01.T01:three_prime_UTR	30.0%
	ATGTTAAGAAGAAGGATATC+AGG	+	Chr3:86500494-86500513	MsG0380016678.01.T01:CDS	30.0%
	GAATAATGTTGTATTAGGTG+AGG	+	Chr3:86500251-86500270	MsG0380016678.01.T01:intron	30.0%
	GGTATTTATGTTAGTGAGAA+GGG	+	Chr3:86500529-86500548	MsG0380016678.01.T01:CDS	30.0%
	TGATATCCTTCTTCTTAACA+TGG	-	Chr3:86500495-86500514	None:intergenic	30.0%
	TGGTATTTATGTTAGTGAGA+AGG	+	Chr3:86500528-86500547	MsG0380016678.01.T01:CDS	30.0%
	TGGTTAAATAATGCACTGAA+TGG	+	Chr3:86500867-86500886	MsG0380016678.01.T01:three_prime_UTR	30.0%
!	TTACATTTGCTTGTTGAGTT+TGG	+	Chr3:86500673-86500692	MsG0380016678.01.T01:three_prime_UTR	30.0%
!	TTTACTAGTTTGTGAATCTG+TGG	+	Chr3:86500219-86500238	MsG0380016678.01.T01:intron	30.0%
!!	CTTGTTGAGTTTGGAATTTT+TGG	+	Chr3:86500682-86500701	MsG0380016678.01.T01:three_prime_UTR	30.0%
!!!	GTCTTCATTTTTTGATACTG+TGG	-	Chr3:86499531-86499550	None:intergenic	30.0%
	AAACTTTCTTGGAGAGAAGA+AGG	+	Chr3:86499893-86499912	MsG0380016678.01.T01:CDS	35.0%
	ATGTTCATGGACCATGTTAA+GGG	-	Chr3:86500400-86500419	None:intergenic	35.0%
	ATTGAGATCCGAAACTTTCT+TGG	+	Chr3:86499882-86499901	MsG0380016678.01.T01:CDS	35.0%
	GAAATGCCATGTTAAGAAGA+AGG	+	Chr3:86500486-86500505	MsG0380016678.01.T01:intron	35.0%
	GATATCAGGAAGTTTCTTGA+TGG	+	Chr3:86500508-86500527	MsG0380016678.01.T01:CDS	35.0%
	TCTTCTCTCCAAGAAAGTTT+CGG	-	Chr3:86499893-86499912	None:intergenic	35.0%
	TTATGTTAGTGAGAAGGGAT+CGG	+	Chr3:86500534-86500553	MsG0380016678.01.T01:CDS	35.0%
	TTCAATTGTTCGATCTGAGA+AGG	+	Chr3:86500297-86500316	MsG0380016678.01.T01:CDS	35.0%
!	AGGAAAGTAGATTTGCTTGA+TGG	+	Chr3:86500271-86500290	MsG0380016678.01.T01:CDS	35.0%
!	ATGACATTGAGCTTGTTTCT+AGG	+	Chr3:86500347-86500366	MsG0380016678.01.T01:CDS	35.0%
!	ATTCAAGTTTCTGTTGGTCT+TGG	+	Chr3:86500613-86500632	MsG0380016678.01.T01:three_prime_UTR	35.0%
!!	GAATGTTGTTGTTGCGTTTT+TGG	+	Chr3:86500459-86500478	MsG0380016678.01.T01:intron	35.0%
!!!	TTGGATTGGTTACTGTTTTC+TGG	+	Chr3:86500632-86500651	MsG0380016678.01.T01:three_prime_UTR	35.0%
	AGACAAACCTCATCTTGTAG+CGG	-	Chr3:86499815-86499834	None:intergenic	40.0%
	CATGTTCATGGACCATGTTA+AGG	-	Chr3:86500401-86500420	None:intergenic	40.0%
	CTTCTTCTTGCCTTCTTCAT+CGG	-	Chr3:86499691-86499710	None:intergenic	40.0%
	GGTGGTAACTGAAGAATAGA+TGG	+	Chr3:86500555-86500574	MsG0380016678.01.T01:exon	40.0%
	GTCATGTGCACTTATTAACC+AGG	+	Chr3:86500369-86500388	MsG0380016678.01.T01:CDS	40.0%
	TAGCATCAAGGTTAACGCTA+AGG	+	Chr3:86499590-86499609	MsG0380016678.01.T01:CDS	40.0%
	TGGAAATCTCACACACTCTA+CGG	-	Chr3:86499511-86499530	None:intergenic	40.0%
	TGTTCATGGACCATGTTAAG+GGG	-	Chr3:86500399-86500418	None:intergenic	40.0%
!!	AGTTTCTGTTGGTCTTGGAT+TGG	+	Chr3:86500618-86500637	MsG0380016678.01.T01:three_prime_UTR	40.0%
!!	CTCAATCGACTTGTTGTCAT+TGG	-	Chr3:86499868-86499887	None:intergenic	40.0%
!!!	AAGGATGAGGTTGTTTTGGA+TGG	+	Chr3:86500322-86500341	MsG0380016678.01.T01:CDS	40.0%
	ATCTGAGAAGGTGAAGGATG+AGG	+	Chr3:86500309-86500328	MsG0380016678.01.T01:CDS	45.0%
	CACGTTGAGAATCTCATCAC+TGG	+	Chr3:86499774-86499793	MsG0380016678.01.T01:CDS	45.0%
	CAGTGATGAGATTCTCAACG+TGG	-	Chr3:86499776-86499795	None:intergenic	45.0%
	CGGTGATGAGATCAAAATCG+AGG	-	Chr3:86499671-86499690	None:intergenic	45.0%
	GAAACCATGAACATCCCTGA+CGG	+	Chr3:86499564-86499583	MsG0380016678.01.T01:CDS	45.0%
	GAAAGCTCAGAGTATGAGTC+TGG	+	Chr3:86501000-86501019	MsG0380016678.01.T01:three_prime_UTR	45.0%
	GATCTCATCACCGATGAAGA+AGG	+	Chr3:86499678-86499697	MsG0380016678.01.T01:CDS	45.0%
	GTCAGGGATGTTCATGGTTT+CGG	-	Chr3:86499565-86499584	None:intergenic	45.0%
	TGTTCGATCTGAGAAGGTGA+AGG	+	Chr3:86500303-86500322	MsG0380016678.01.T01:CDS	45.0%
!	GGTGAAGGATGAGGTTGTTT+TGG	+	Chr3:86500318-86500337	MsG0380016678.01.T01:CDS	45.0%
!!	ATTGGTGATGCTAGCGTTGA+TGG	-	Chr3:86499850-86499869	None:intergenic	45.0%
!!	TTGGTGATGCTAGCGTTGAT+GGG	-	Chr3:86499849-86499868	None:intergenic	45.0%
	ACCAGGTACACCCCTTAACA+TGG	+	Chr3:86500386-86500405	MsG0380016678.01.T01:intron	50.0%
	AGAAGCTCAAGATCGATGCC+TGG	+	Chr3:86499706-86499725	MsG0380016678.01.T01:CDS	50.0%
	AGGGATGTTCATGGTTTCGG+AGG	-	Chr3:86499562-86499581	None:intergenic	50.0%
	AGGGTTTCCGCTACAAGATG+AGG	+	Chr3:86499805-86499824	MsG0380016678.01.T01:CDS	50.0%
	CTCAAGATCGATGCCTGGTT+CGG	+	Chr3:86499711-86499730	MsG0380016678.01.T01:CDS	50.0%
	GCTAAGGTCATCGAAGTCGA+AGG	+	Chr3:86499606-86499625	MsG0380016678.01.T01:CDS	50.0%
	TCTGTGTTTGCAGCACTCTC+CGG	-	Chr3:86499484-86499503	None:intergenic	50.0%
	TGTTAGTGAGAAGGGATCGG+TGG	+	Chr3:86500537-86500556	MsG0380016678.01.T01:CDS	50.0%
	TGTTCATGGTTTCGGAGGAG+AGG	-	Chr3:86499557-86499576	None:intergenic	50.0%
!	ACCATGTTAAGGGGTGTACC+TGG	-	Chr3:86500390-86500409	None:intergenic	50.0%
!	CATCTTGTAGCGGAAACCCT+TGG	-	Chr3:86499805-86499824	None:intergenic	50.0%
!!	CTCATCACTGGTGTCACCAA+GGG	+	Chr3:86499786-86499805	MsG0380016678.01.T01:CDS	50.0%
!!	TCTCATCACTGGTGTCACCA+AGG	+	Chr3:86499785-86499804	MsG0380016678.01.T01:CDS	50.0%
!!	TGGTGATGCTAGCGTTGATG+GGG	-	Chr3:86499848-86499867	None:intergenic	50.0%
	AACGCCGTCAGGGATGTTCA+TGG	-	Chr3:86499571-86499590	None:intergenic	55.0%
	ACCTTGATGCTAACGCCGTC+AGG	-	Chr3:86499582-86499601	None:intergenic	55.0%
	ATCGAAGTCGAAGGACCTCG+CGG	+	Chr3:86499615-86499634	MsG0380016678.01.T01:CDS	55.0%
	CATGGTTTCGGAGGAGAGGA+TGG	-	Chr3:86499553-86499572	None:intergenic	55.0%
	CCTTGATGCTAACGCCGTCA+GGG	-	Chr3:86499581-86499600	None:intergenic	55.0%
!	GCTGAGAGCAGTTCTGATGG+CGG	-	Chr3:86499754-86499773	None:intergenic	55.0%
!	GGTTTTCCTGGAACCGAACC+AGG	-	Chr3:86499727-86499746	None:intergenic	55.0%
!	GTGGCTGAGAGCAGTTCTGA+TGG	-	Chr3:86499757-86499776	None:intergenic	55.0%
	CCCTGACGGCGTTAGCATCA+AGG	+	Chr3:86499578-86499597	MsG0380016678.01.T01:CDS	60.0%
	TCACGAACAAGCTTCCCGCG+AGG	-	Chr3:86499633-86499652	None:intergenic	60.0%
	TCGAAGTCGAAGGACCTCGC+GGG	+	Chr3:86499616-86499635	MsG0380016678.01.T01:CDS	60.0%
!!	GAGAGCAGTTCTGATGGCGG+CGG	-	Chr3:86499751-86499770	None:intergenic	60.0%
!!	TCGATGCCTGGTTCGGTTCC+AGG	+	Chr3:86499718-86499737	MsG0380016678.01.T01:CDS	60.0%
!!	AGCAGTTCTGATGGCGGCGG+AGG	-	Chr3:86499748-86499767	None:intergenic	65.0%
!!	GATGGCGGCGGAGGTTTTCC+TGG	-	Chr3:86499739-86499758	None:intergenic	65.0%

Chromosome	Type	Strat	End	Strand	Name
Chr3	gene	86499478	86501023	86499478	ID=MsG0380016678.01;Name=MsG0380016678.01
Chr3	mRNA	86499478	86501023	86499478	ID=MsG0380016678.01.T01;Parent=MsG0380016678.01;Name=MsG0380016678.01.T01;_AED=0.48;_eAED=0.48;_QI=65\|1\|1\|1\|1\|1\|3\|450\|193
Chr3	exon	86499478	86499914	86499478	ID=MsG0380016678.01.T01:exon:21857;Parent=MsG0380016678.01.T01
Chr3	exon	86500268	86500390	86500268	ID=MsG0380016678.01.T01:exon:21858;Parent=MsG0380016678.01.T01
Chr3	exon	86500487	86501023	86500487	ID=MsG0380016678.01.T01:exon:21859;Parent=MsG0380016678.01.T01
Chr3	five_prime_UTR	86499478	86499542	86499478	ID=MsG0380016678.01.T01:five_prime_utr;Parent=MsG0380016678.01.T01
Chr3	CDS	86499543	86499914	86499543	ID=MsG0380016678.01.T01:cds;Parent=MsG0380016678.01.T01
Chr3	CDS	86500268	86500390	86500268	ID=MsG0380016678.01.T01:cds;Parent=MsG0380016678.01.T01
Chr3	CDS	86500487	86500573	86500487	ID=MsG0380016678.01.T01:cds;Parent=MsG0380016678.01.T01
Chr3	three_prime_UTR	86500574	86501023	86500574	ID=MsG0380016678.01.T01:three_prime_utr;Parent=MsG0380016678.01.T01

Gene Sequence

>MsG0380016678.01.T01

ATGAAGACCATCCTCTCCTCCGAAACCATGAACATCCCTGACGGCGTTAGCATCAAGGTTAACGCTAAGGTCATCGAAGTCGAAGGACCTCGCGGGAAGCTTGTTCGTGATTTCAAGCATCTCAACCTCGATTTTGATCTCATCACCGATGAAGAAGGCAAGAAGAAGCTCAAGATCGATGCCTGGTTCGGTTCCAGGAAAACCTCCGCCGCCATCAGAACTGCTCTCAGCCACGTTGAGAATCTCATCACTGGTGTCACCAAGGGTTTCCGCTACAAGATGAGGTTTGTCTATGCTCATTTCCCCATCAACGCTAGCATCACCAATGACAACAAGTCGATTGAGATCCGAAACTTTCTTGGAGAGAAGAAGGTGAGGAAAGTAGATTTGCTTGATGGAGTTTCAATTGTTCGATCTGAGAAGGTGAAGGATGAGGTTGTTTTGGATGGAAATGACATTGAGCTTGTTTCTAGGTCATGTGCACTTATTAACCAGAAATGCCATGTTAAGAAGAAGGATATCAGGAAGTTTCTTGATGGTATTTATGTTAGTGAGAAGGGATCGGTGGTAACTGAAGAATAG

Protein sequence

>MsG0380016678.01.T01

MKTILSSETMNIPDGVSIKVNAKVIEVEGPRGKLVRDFKHLNLDFDLITDEEGKKKLKIDAWFGSRKTSAAIRTALSHVENLITGVTKGFRYKMRFVYAHFPINASITNDNKSIEIRNFLGEKKVRKVDLLDGVSIVRSEKVKDEVVLDGNDIELVSRSCALINQKCHVKKKDIRKFLDGIYVSEKGSVVTEE*