AlfalfaGEDB | MsG0180001255.01

M. sativa cultivar ZhongmuNo.1 / MsG0180001255.01

KEGG: -
GO: GO:0003676
PFAM: PF13456

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180001255.01.T02	AES59299.1	67.568	74	24	0	2	75	78	151	2.91E-26	104
MsG0180001255.01.T01	XP_024642160.1	44.262	183	72	2	1	153	3	185	8.64E-42	149

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180001255.01.T01	Q2HVU8	48.603	179	66	4	1	153	369	547	1.47e-41	155
MsG0180001255.01.T02	Q2HVU8	64.865	74	26	0	2	75	474	547	1.00e-24	105

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI

Gene1	Gene2	Type
MsG0780040274.01	MsG0180001255.01	PPI
MsG0180001255.01	MsG0780041116.01	PPI
MsG0180001255.01	MsG0780040274.01	PPI
MsG0180001255.01	MsG0780041116.01	PPI
MsG0180001255.01	MsG0380013578.01	PPI
MsG0180001255.01	MsG0780041116.01	PPI
MsG0180001255.01	MsG0780041116.01	PPI
MsG0180001255.01	MsG0280009322.01	PPI
MsG0180001255.01	MsG0280009322.01	PPI
MsG0180001255.01	MsG0280010449.01	PPI
MsG0180001255.01	MsG0380013578.01	PPI
MsG0180001255.01	MsG0280010449.01	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180001255.01.T02	MTR_1g017840	67.568	74	24	0	2	75	78	151	3.52e-30	104
MsG0180001255.01.T02	MTR_0093s0070	64.789	71	25	0	5	75	126	196	2.76e-29	103
MsG0180001255.01.T02	MTR_8g098925	62.857	70	26	0	6	75	69	138	6.69e-28	98.6
MsG0180001255.01.T02	MTR_6g089560	62.162	74	28	0	2	75	116	189	7.02e-28	100
MsG0180001255.01.T02	MTR_8g099580	55.405	74	33	0	2	75	206	279	1.73e-23	90.5
MsG0180001255.01.T02	MTR_5g037920	59.459	74	29	1	2	75	98	170	2.45e-23	88.6
MsG0180001255.01.T02	MTR_7g013520	57.333	75	32	0	1	75	69	143	6.70e-23	86.3
MsG0180001255.01.T02	MTR_2g059470	55.556	72	32	0	4	75	2	73	1.11e-22	83.2
MsG0180001255.01.T02	MTR_7g063280	47.297	74	39	0	2	75	72	145	2.70e-22	84.7
MsG0180001255.01.T02	MTR_1g034430	51.429	70	34	0	1	70	94	163	1.10e-20	80.9
MsG0180001255.01.T02	MTR_6g016265	47.297	74	39	0	2	75	39	112	2.71e-20	78.2
MsG0180001255.01.T02	MTR_1g074240	59.322	59	24	0	4	62	2	60	1.55e-19	74.7
MsG0180001255.01.T02	MTR_6g023630	74.510	51	12	1	15	64	2	52	1.94e-19	74.7
MsG0180001255.01.T02	MTR_5g049680	50.769	65	32	0	1	65	30	94	2.22e-19	75.5
MsG0180001255.01.T02	MTR_0015s0120	49.333	75	38	0	1	75	292	366	3.22e-19	79.7
MsG0180001255.01.T02	MTR_6g453270	50.704	71	34	1	2	71	300	370	5.32e-19	79.0
MsG0180001255.01.T02	MTR_3g116210	45.333	75	41	0	1	75	96	170	1.00e-18	75.9
MsG0180001255.01.T02	MTR_4g008270	48.529	68	35	0	2	69	10	77	1.31e-18	73.2
MsG0180001255.01.T02	MTR_5g034450	53.333	60	28	0	2	61	49	108	2.04e-18	73.2
MsG0180001255.01.T02	MTR_8g031800	47.826	69	36	0	1	69	30	98	2.84e-18	72.8
MsG0180001255.01.T02	MTR_7g053400	47.887	71	30	1	6	69	179	249	3.83e-18	75.5
MsG0180001255.01.T02	MTR_5g036320	53.030	66	31	0	2	67	79	144	6.00e-18	72.8
MsG0180001255.01.T02	MTR_4g029360	52.703	74	35	0	2	75	127	200	7.55e-18	74.3
MsG0180001255.01.T02	MTR_1g022215	50.877	57	28	0	2	58	14	70	8.49e-18	70.9
MsG0180001255.01.T02	MTR_8g059135	45.070	71	39	0	5	75	31	101	8.59e-18	72.0
MsG0180001255.01.T02	MTR_1g088650	53.125	64	30	0	2	65	86	149	3.11e-17	72.0
MsG0180001255.01.T02	MTR_1g074090	44.776	67	37	0	1	67	80	146	1.03e-16	69.7
MsG0180001255.01.T02	MTR_2g027020	51.852	54	26	0	2	55	28	81	1.59e-16	70.1
MsG0180001255.01.T02	MTR_1g069475	42.667	75	43	0	1	75	163	237	3.95e-16	70.1
MsG0180001255.01.T02	MTR_5g085210	44.000	75	42	0	1	75	118	192	5.56e-16	69.3
MsG0180001255.01.T02	MTR_5g075610	48.485	66	32	1	8	73	1	64	9.63e-16	65.9
MsG0180001255.01.T02	MTR_2g055490	56.140	57	25	0	7	63	12	68	1.42e-15	65.9
MsG0180001255.01.T02	MTR_6g016355	48.333	60	31	0	2	61	116	175	1.68e-15	67.4
MsG0180001255.01.T02	MTR_1g050488	44.615	65	36	0	1	65	97	161	2.10e-15	66.6
MsG0180001255.01.T02	MTR_6g016135	48.333	60	31	0	6	65	383	442	7.33e-15	67.8
MsG0180001255.01.T02	MTR_8g036780	46.667	60	32	0	1	60	33	92	1.79e-14	63.2
MsG0180001255.01.T02	MTR_1g045340	40.000	75	45	0	1	75	83	157	2.87e-14	63.9
MsG0180001255.01.T02	MTR_5g083200	40.000	75	45	0	1	75	97	171	4.87e-14	63.5
MsG0180001255.01.T02	MTR_0002s1220	40.000	75	45	0	1	75	97	171	4.87e-14	63.5
MsG0180001255.01.T02	MTR_1g099430	43.103	58	31	1	1	56	124	181	1.71e-13	62.4
MsG0180001255.01.T02	MTR_3g111390	38.667	75	46	0	1	75	97	171	1.96e-13	62.0
MsG0180001255.01.T02	MTR_1g070820	38.667	75	46	0	1	75	97	171	1.96e-13	62.0
MsG0180001255.01.T02	MTR_6g046670	54.545	55	25	0	1	55	122	176	2.25e-13	62.0
MsG0180001255.01.T02	MTR_1g062840	50.980	51	25	0	25	75	3	53	4.51e-13	58.9
MsG0180001255.01.T02	MTR_6g033225	43.077	65	37	0	6	70	74	138	5.84e-13	59.7
MsG0180001255.01.T02	MTR_4g094272	56.250	48	20	1	2	49	94	140	6.48e-13	59.7
MsG0180001255.01.T02	MTR_1g045560	38.710	62	38	0	8	69	1	62	7.61e-13	58.5
MsG0180001255.01.T02	MTR_6g034915	35.616	73	47	0	3	75	56	128	1.12e-12	59.7
MsG0180001255.01.T02	MTR_1g081930	40.541	74	44	0	1	74	37	110	1.54e-12	58.5
MsG0180001255.01.T02	MTR_8g088220	43.750	64	36	0	12	75	4	67	1.65e-12	57.0
MsG0180001255.01.T02	MTR_3g026270	44.231	52	29	0	10	61	3	54	1.99e-12	56.6
MsG0180001255.01.T02	MTR_2g017550	51.923	52	25	0	4	55	110	161	2.64e-12	58.5
MsG0180001255.01.T02	MTR_1g109000	45.902	61	33	0	2	62	26	86	3.09e-12	56.6
MsG0180001255.01.T02	MTR_8g079540	43.333	60	34	0	2	61	48	107	3.47e-12	57.4
MsG0180001255.01.T02	MTR_8g031080	59.524	42	17	0	2	43	289	330	5.05e-12	59.7
MsG0180001255.01.T02	MTR_8g038340	45.763	59	32	0	1	59	188	246	1.62e-11	57.8
MsG0180001255.01.T02	MTR_3g462490	58.537	41	17	0	1	41	100	140	1.91e-11	57.8
MsG0180001255.01.T02	MTR_5g430460	83.871	31	5	0	1	31	217	247	2.51e-11	57.0
MsG0180001255.01.T02	MTR_7g078610	56.818	44	19	0	1	44	50	93	4.68e-11	53.9
MsG0180001255.01.T02	MTR_1g018300	45.333	75	35	1	1	75	62	130	8.77e-11	55.8
MsG0180001255.01.T01	MTR_4g029360	45.570	158	81	1	1	153	43	200	4.71e-39	132
MsG0180001255.01.T01	MTR_1g017840	48.366	153	54	4	22	153	3	151	5.95e-37	124
MsG0180001255.01.T01	MTR_6g453270	42.236	161	81	3	1	149	210	370	5.48e-33	120
MsG0180001255.01.T01	MTR_6g089560	60.000	90	35	1	65	153	100	189	2.50e-32	115
MsG0180001255.01.T01	MTR_7g013520	45.926	135	62	2	19	153	20	143	6.60e-31	110
MsG0180001255.01.T01	MTR_2g017550	40.940	149	72	2	1	133	13	161	1.41e-30	108
MsG0180001255.01.T01	MTR_8g099580	43.885	139	68	3	25	153	141	279	2.09e-30	112
MsG0180001255.01.T01	MTR_0093s0070	40.789	152	60	3	14	153	63	196	2.42e-30	109
MsG0180001255.01.T01	MTR_1g034430	39.474	152	84	3	2	148	15	163	2.46e-29	105
MsG0180001255.01.T01	MTR_5g037920	44.853	136	65	4	24	153	39	170	6.38e-29	106
MsG0180001255.01.T01	MTR_1g054905	43.182	132	66	3	1	123	13	144	6.51e-29	104
MsG0180001255.01.T01	MTR_6g046670	40.141	142	76	2	1	133	35	176	6.64e-29	105
MsG0180001255.01.T01	MTR_8g031080	42.222	135	61	3	1	121	199	330	9.29e-28	106
MsG0180001255.01.T01	MTR_8g098925	42.029	138	67	2	29	153	1	138	1.13e-27	101
MsG0180001255.01.T01	MTR_1g050488	38.462	143	75	2	1	143	32	161	1.25e-27	101
MsG0180001255.01.T01	MTR_3g116210	36.842	133	77	2	21	153	45	170	2.12e-26	98.6
MsG0180001255.01.T01	MTR_5g049680	47.312	93	43	1	51	143	8	94	4.14e-25	92.8
MsG0180001255.01.T01	MTR_6g016265	49.438	89	44	1	66	153	24	112	5.47e-25	93.2
MsG0180001255.01.T01	MTR_0015s0120	51.087	92	44	1	63	153	275	366	3.25e-24	96.7
MsG0180001255.01.T01	MTR_4g046027	45.082	122	45	3	1	122	70	169	4.60e-24	92.0
MsG0180001255.01.T01	MTR_6g045427	35.152	165	67	3	1	153	82	218	1.69e-23	92.4
MsG0180001255.01.T01	MTR_1g087480	43.860	114	55	2	1	112	114	220	1.78e-23	92.0
MsG0180001255.01.T01	MTR_5g034450	51.190	84	36	1	63	141	27	110	1.83e-23	89.0
MsG0180001255.01.T01	MTR_7g053400	35.065	154	68	4	1	147	121	249	1.87e-23	92.8
MsG0180001255.01.T01	MTR_1g088650	38.129	139	76	3	5	143	21	149	4.74e-23	90.1
MsG0180001255.01.T01	MTR_8g031800	45.000	100	49	1	49	148	6	99	4.99e-23	87.8
MsG0180001255.01.T01	MTR_5g036320	40.833	120	65	1	26	145	31	144	3.31e-22	86.7
MsG0180001255.01.T01	MTR_1g074090	49.351	77	39	0	69	145	70	146	1.38e-21	85.1
MsG0180001255.01.T01	MTR_2g059470	55.556	72	32	0	82	153	2	73	1.58e-21	83.2
MsG0180001255.01.T01	MTR_7g063280	47.297	74	39	0	80	153	72	145	2.30e-21	85.5
MsG0180001255.01.T01	MTR_1g022215	50.746	67	33	0	70	136	4	70	1.01e-20	81.3
MsG0180001255.01.T01	MTR_1g045340	36.719	128	75	2	32	153	30	157	1.39e-20	83.6
MsG0180001255.01.T01	MTR_2g027020	52.113	71	33	1	64	133	11	81	2.15e-20	83.2
MsG0180001255.01.T01	MTR_1g069475	44.706	85	47	0	69	153	153	237	1.13e-19	82.4
MsG0180001255.01.T01	MTR_5g083200	35.294	119	69	2	37	153	59	171	1.48e-19	81.3
MsG0180001255.01.T01	MTR_0002s1220	35.294	119	69	2	37	153	59	171	1.48e-19	81.3
MsG0180001255.01.T01	MTR_8g036780	41.237	97	51	1	42	138	2	92	3.34e-19	78.2
MsG0180001255.01.T01	MTR_1g099430	49.315	73	34	2	65	134	109	181	5.34e-19	79.7
MsG0180001255.01.T01	MTR_8g028785	35.329	167	67	4	1	153	192	331	5.69e-19	82.0
MsG0180001255.01.T01	MTR_6g023630	74.510	51	12	1	93	142	2	52	7.03e-19	76.3
MsG0180001255.01.T01	MTR_6g016355	47.222	72	38	0	68	139	104	175	9.01e-19	78.6
MsG0180001255.01.T01	MTR_5g085210	44.706	85	47	0	69	153	108	192	1.17e-18	79.7
MsG0180001255.01.T01	MTR_1g074240	59.322	59	24	0	82	140	2	60	1.58e-18	75.1
MsG0180001255.01.T01	MTR_3g111390	33.613	119	71	2	37	153	59	171	2.03e-18	78.2
MsG0180001255.01.T01	MTR_1g070820	33.613	119	71	2	37	153	59	171	2.03e-18	78.2
MsG0180001255.01.T01	MTR_4g094272	59.322	59	23	1	69	127	83	140	4.16e-18	75.9
MsG0180001255.01.T01	MTR_1g081930	41.758	91	53	0	62	152	20	110	4.70e-18	75.5
MsG0180001255.01.T01	MTR_4g008270	45.833	72	39	0	76	147	6	77	1.62e-17	73.2
MsG0180001255.01.T01	MTR_8g079540	46.479	71	38	0	69	139	37	107	1.74e-17	73.9
MsG0180001255.01.T01	MTR_8g039080	35.000	160	81	6	1	151	27	172	2.31e-17	75.9
MsG0180001255.01.T01	MTR_8g059135	35.780	109	60	2	45	153	3	101	2.43e-17	73.6
MsG0180001255.01.T01	MTR_6g033225	36.522	115	72	1	35	148	24	138	3.56e-17	73.6
MsG0180001255.01.T01	MTR_1g109000	48.611	72	37	0	69	140	15	86	5.41e-17	71.6
MsG0180001255.01.T01	MTR_8g038340	46.341	82	38	1	56	137	171	246	6.45e-17	75.1
MsG0180001255.01.T01	MTR_5g430460	85.366	41	6	0	69	109	207	247	1.82e-16	73.9
MsG0180001255.01.T01	MTR_3g462490	60.714	56	21	1	65	119	85	140	1.99e-16	75.5
MsG0180001255.01.T01	MTR_4g035890	34.513	113	69	2	2	114	41	148	8.51e-16	70.1
MsG0180001255.01.T01	MTR_7g078610	49.254	67	28	1	56	122	33	93	1.28e-15	68.2
MsG0180001255.01.T01	MTR_2g047100	45.055	91	34	2	64	153	1	76	1.89e-15	68.2
MsG0180001255.01.T01	MTR_1g018300	41.228	114	55	2	40	153	29	130	4.02e-15	70.5
MsG0180001255.01.T01	MTR_2g102247	39.000	100	54	2	1	99	55	148	4.33e-15	68.6
MsG0180001255.01.T01	MTR_5g075610	48.485	66	32	1	86	151	1	64	9.03e-15	66.2
MsG0180001255.01.T01	MTR_6g034915	32.787	122	51	2	63	153	7	128	1.13e-14	67.8
MsG0180001255.01.T01	MTR_4g094370	53.704	54	25	0	69	122	24	77	1.61e-14	65.1
MsG0180001255.01.T01	MTR_2g055490	56.140	57	25	0	85	141	12	68	2.42e-14	65.5
MsG0180001255.01.T01	MTR_6g016135	48.333	60	31	0	84	143	383	442	7.90e-14	68.2
MsG0180001255.01.T01	MTR_8g022750	37.500	104	42	3	45	147	174	255	3.88e-13	65.5
MsG0180001255.01.T01	MTR_3g102000	38.776	98	54	3	11	103	130	226	4.97e-13	64.3
MsG0180001255.01.T01	MTR_1g062840	49.057	53	27	0	101	153	1	53	5.50e-12	58.9
MsG0180001255.01.T01	MTR_8g045330	35.644	101	61	1	28	124	41	141	5.77e-12	60.5
MsG0180001255.01.T01	MTR_1g045560	38.710	62	38	0	86	147	1	62	9.91e-12	58.2
MsG0180001255.01.T01	MTR_8g088220	43.750	64	36	0	90	153	4	67	1.39e-11	57.4
MsG0180001255.01.T01	MTR_7g036825	34.375	96	56	2	1	95	111	200	2.50e-11	59.7
MsG0180001255.01.T01	MTR_3g026270	44.231	52	29	0	88	139	3	54	3.28e-11	56.2
MsG0180001255.01.T01	MTR_5g042170	76.471	34	8	0	70	103	40	73	6.16e-11	56.6
MsG0180001255.01.T01	MTR_7g006380	46.429	56	29	1	65	119	50	105	7.95e-11	57.8

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 23 sgRNAs with CRISPR-Local

Find 27 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CAGCCATTTGCTAGAGCTTA+TGG	0.247639	1:-18376406	None:intergenic
TGTTCATGTTGGTGAAGCTT+TGG	0.344327	1:-18376033	MsG0180001255.01.T01:CDS
TGACGAGAATCTCGACGAAA+TGG	0.376799	1:+18376226	None:intergenic
GTTCATGTTGGTGAAGCTTT+GGG	0.383061	1:-18376032	MsG0180001255.01.T01:CDS
CCTTTATGTTCTGTTCATGT+TGG	0.418283	1:-18376044	MsG0180001255.01.T01:CDS
ATTTGAGTGTAAAGCGTCAT+TGG	0.427729	1:+18375935	None:intergenic
GACGAGAATCTCGACGAAAT+GGG	0.437397	1:+18376227	None:intergenic
CGCGTCACCTCATTGAAGAT+TGG	0.460022	1:-18376319	MsG0180001255.01.T01:CDS
GTTTATTTAATGATATAGAA+TGG	0.466041	1:-18376010	MsG0180001255.01.T01:CDS
CCAACATGAACAGAACATAA+AGG	0.480235	1:+18376044	None:intergenic
TCGAGATTCTCGTCAATAGC+AGG	0.485331	1:-18376218	MsG0180001255.01.T01:CDS
GTGAAGCTGAAGTGGTTTGT+TGG	0.507151	1:+18376261	None:intergenic
TCTTCCAGAGTTTAAAGTTG+TGG	0.507698	1:+18376378	None:intergenic
TCTATGAATCGTGTAGGCAT+TGG	0.550934	1:-18376125	MsG0180001255.01.T01:CDS
CCTCATGTGTGAAGCTGAAG+TGG	0.555158	1:+18376253	None:intergenic
AGCACCACAACTTTAAACTC+TGG	0.558494	1:-18376382	MsG0180001255.01.T01:CDS
TCGCAGTCTATGAATCGTGT+AGG	0.568719	1:-18376131	MsG0180001255.01.T01:CDS
ATGGTTAAGTGACATGCAGA+TGG	0.578013	1:-18375991	MsG0180001255.01.T01:CDS
GATGGACAATGTCGATTTCG+TGG	0.578325	1:-18375973	MsG0180001255.01.T01:CDS
CCACTTCAGCTTCACACATG+AGG	0.592754	1:-18376253	MsG0180001255.01.T01:CDS
CATCATGCCAATCTTCAATG+AGG	0.661776	1:+18376312	None:intergenic
GGACAATGTCGATTTCGTGG+TGG	0.683532	1:-18375970	MsG0180001255.01.T01:CDS
ACGCGCTCTATCAACTACGT+TGG	0.686342	1:+18376337	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	GTTTATTTAATGATATAGAA+TGG	-	Chr1:18376299-18376318	MsG0180001255.01.T01:CDS	15.0%
	ACATAAAGGAGAAAAACTCA+AGG	+	Chr1:18376254-18376273	None:intergenic	30.0%
	TGATGTACATTAGCAAAGTT+TGG	-	Chr1:18376117-18376136	MsG0180001255.01.T01:CDS	30.0%
	CCAACATGAACAGAACATAA+AGG	+	Chr1:18376268-18376287	None:intergenic	35.0%
	CCTTTATGTTCTGTTCATGT+TGG	-	Chr1:18376265-18376284	MsG0180001255.01.T01:CDS	35.0%
!	AGGAGAAAAACTCAAGGTTT+TGG	+	Chr1:18376248-18376267	None:intergenic	35.0%
!	ATTTGAGTGTAAAGCGTCAT+TGG	+	Chr1:18376377-18376396	None:intergenic	35.0%
!	TCTTCCAGAGTTTAAAGTTG+TGG	+	Chr1:18375934-18375953	None:intergenic	35.0%
	AAGCATCAATGTTGCACTTG+AGG	+	Chr1:18376153-18376172	None:intergenic	40.0%
	AGCACCACAACTTTAAACTC+TGG	-	Chr1:18375927-18375946	MsG0180001255.01.T01:CDS	40.0%
	ATGGTTAAGTGACATGCAGA+TGG	-	Chr1:18376318-18376337	MsG0180001255.01.T01:CDS	40.0%
	CATCATGCCAATCTTCAATG+AGG	+	Chr1:18376000-18376019	None:intergenic	40.0%
	TCTATGAATCGTGTAGGCAT+TGG	-	Chr1:18376184-18376203	MsG0180001255.01.T01:intron	40.0%
!	GTTCATGTTGGTGAAGCTTT+GGG	-	Chr1:18376277-18376296	MsG0180001255.01.T01:CDS	40.0%
!	TGTTCATGTTGGTGAAGCTT+TGG	-	Chr1:18376276-18376295	MsG0180001255.01.T01:CDS	40.0%
	GACGAGAATCTCGACGAAAT+GGG	+	Chr1:18376085-18376104	None:intergenic	45.0%
	TCGAGATTCTCGTCAATAGC+AGG	-	Chr1:18376091-18376110	MsG0180001255.01.T01:CDS	45.0%
	TCGCAGTCTATGAATCGTGT+AGG	-	Chr1:18376178-18376197	MsG0180001255.01.T01:intron	45.0%
	TGACGAGAATCTCGACGAAA+TGG	+	Chr1:18376086-18376105	None:intergenic	45.0%
!	GTGAAGCTGAAGTGGTTTGT+TGG	+	Chr1:18376051-18376070	None:intergenic	45.0%
!!	GATGGACAATGTCGATTTCG+TGG	-	Chr1:18376336-18376355	MsG0180001255.01.T01:CDS	45.0%
	ACGCGCTCTATCAACTACGT+TGG	+	Chr1:18375975-18375994	None:intergenic	50.0%
	ATGTTGCACTTGAGGCGACT+AGG	+	Chr1:18376145-18376164	None:intergenic	50.0%
	CCACTTCAGCTTCACACATG+AGG	-	Chr1:18376056-18376075	MsG0180001255.01.T01:CDS	50.0%
	CCTCATGTGTGAAGCTGAAG+TGG	+	Chr1:18376059-18376078	None:intergenic	50.0%
	CGCGTCACCTCATTGAAGAT+TGG	-	Chr1:18375990-18376009	MsG0180001255.01.T01:CDS	50.0%
!!	GGACAATGTCGATTTCGTGG+TGG	-	Chr1:18376339-18376358	MsG0180001255.01.T01:CDS	50.0%

Chromosome	Type	Strat	End	Strand	Name
Chr1	gene	18375917	18376414	18375917	ID=MsG0180001255.01;Name=MsG0180001255.01
Chr1	mRNA	18375917	18376414	18375917	ID=MsG0180001255.01.T01;Parent=MsG0180001255.01;Name=MsG0180001255.01.T01;_AED=0.28;_eAED=0.28;_QI=0\|0\|0\|1\|0\|0\|2\|0\|153
Chr1	exon	18375917	18376174	18375917	ID=MsG0180001255.01.T01:exon:32436;Parent=MsG0180001255.01.T01
Chr1	exon	18376211	18376414	18376211	ID=MsG0180001255.01.T01:exon:32435;Parent=MsG0180001255.01.T01
Chr1	CDS	18376211	18376414	18376211	ID=MsG0180001255.01.T01:cds;Parent=MsG0180001255.01.T01
Chr1	CDS	18375917	18376174	18375917	ID=MsG0180001255.01.T01:cds;Parent=MsG0180001255.01.T01
Chr1	mRNA	18375917	18376144	18375917	ID=MsG0180001255.01.T02;Parent=MsG0180001255.01;Name=MsG0180001255.01.T02;_AED=0.39;_eAED=0.39;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|75
Chr1	exon	18375917	18376144	18375917	ID=MsG0180001255.01.T02:exon:32437;Parent=MsG0180001255.01.T02
Chr1	CDS	18375917	18376144	18375917	ID=MsG0180001255.01.T02:cds;Parent=MsG0180001255.01.T02

Gene Sequence

>MsG0180001255.01.T02

ATGAATCGTGTAGGCATTGGTATATGTGTGCGCGACGATGATGAAGCTTTTGTTCTAGCCAAAACCTTGAGTTTTTCTCCTTTATGTTCTGTTCATGTTGGTGAAGCTTTGGGTTTATTTAATGATATAGAATGGTTAAGTGACATGCAGATGGACAATGTCGATTTCGTGGTGGATTCTAAAACAACCAATGACGCTTTACACTCAAATCGACTTGATGTTTCATAA

>MsG0180001255.01.T01

AGCTTATGGAAGCACCACAACTTTAAACTCTGGAAGAATGAGAATGAACTGTGTGCCAACGTAGTTGATAGAGCGCGTCACCTCATTGAAGATTGGCATGATGCTAATATCAGTCATAGTGCGAGTGATGACCAACAAACCACTTCAGCTTCACACATGAGGCTTCCCATTTCGTCGAGATTCTCGTCAATAGCAGGTCGTGATTGCAACATTGATGCTTCTTTTTCGCAGTCTATGAATCGTGTAGGCATTGGTATATGTGTGCGCGACGATGATGAAGCTTTTGTTCTAGCCAAAACCTTGAGTTTTTCTCCTTTATGTTCTGTTCATGTTGGTGAAGCTTTGGGTTTATTTAATGATATAGAATGGTTAAGTGACATGCAGATGGACAATGTCGATTTCGTGGTGGATTCTAAAACAACCAATGACGCTTTACACTCAAATCGACTTGATGTTTCATAA

Protein sequence

>MsG0180001255.01.T02

MNRVGIGICVRDDDEAFVLAKTLSFSPLCSVHVGEALGLFNDIEWLSDMQMDNVDFVVDSKTTNDALHSNRLDVS*

>MsG0180001255.01.T01

SLWKHHNFKLWKNENELCANVVDRARHLIEDWHDANISHSASDDQQTTSASHMRLPISSRFSSIAGRDCNIDASFSQSMNRVGIGICVRDDDEAFVLAKTLSFSPLCSVHVGEALGLFNDIEWLSDMQMDNVDFVVDSKTTNDALHSNRLDVS*