Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180001297.01.T01 | XP_003589683.1 | 97.316 | 1006 | 24 | 1 | 1 | 1006 | 1 | 1003 | 0 | 2028 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180001297.01 | MsG0180006161.01 | 0.865304 | 6.509385e-65 | 2.107527e-61 |
| MsG0180001297.01 | MsG0380016386.01 | 0.840750 | 7.202282e-58 | 1.035916e-54 |
| MsG0180001297.01 | MsG0380016675.01 | 0.803075 | 4.144344e-49 | 2.063166e-46 |
| MsG0180001297.01 | MsG0380017013.01 | 0.800502 | 1.398564e-48 | 6.522527e-46 |
| MsG0180001297.01 | MsG0780038743.01 | 0.854372 | 1.286578e-61 | 2.866750e-58 |
| MsG0180001297.01 | MsG0780041276.01 | 0.850379 | 1.764011e-60 | 3.448660e-57 |
| MsG0180001297.01 | MsG0780041621.01 | 0.821962 | 3.054693e-53 | 2.522146e-50 |
| MsG0180001297.01 | MsG0880041982.01 | 0.830175 | 3.405358e-55 | 3.560666e-52 |
| MsG0180001297.01 | MsG0880042379.01 | 0.833640 | 4.749587e-56 | 5.502499e-53 |
| MsG0180001297.01 | MsG0880042830.01 | 0.856913 | 2.332644e-62 | 5.655838e-59 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180001297.01.T01 | MTR_1g035010 | 97.316 | 1006 | 24 | 1 | 1 | 1006 | 1 | 1003 | 0.0 | 2028 |
| MsG0180001297.01.T01 | MTR_7g110320 | 33.612 | 598 | 356 | 13 | 437 | 1005 | 415 | 1000 | 1.63e-92 | 317 |
| MsG0180001297.01.T01 | MTR_7g110320 | 62.698 | 126 | 44 | 1 | 77 | 199 | 112 | 237 | 5.40e-40 | 160 |
| MsG0180001297.01.T01 | MTR_1g086250 | 33.393 | 563 | 341 | 11 | 469 | 1005 | 256 | 810 | 1.71e-89 | 304 |
| MsG0180001297.01.T01 | MTR_1g086250 | 33.393 | 563 | 341 | 11 | 469 | 1005 | 448 | 1002 | 2.26e-87 | 303 |
| MsG0180001297.01.T01 | MTR_1g086250 | 62.069 | 116 | 43 | 1 | 77 | 192 | 117 | 231 | 2.72e-36 | 149 |
| MsG0180001297.01.T01 | MTR_2g046550 | 31.795 | 585 | 334 | 18 | 467 | 1006 | 226 | 790 | 1.74e-84 | 290 |
| MsG0180001297.01.T01 | MTR_2g046550 | 31.795 | 585 | 334 | 18 | 467 | 1006 | 461 | 1025 | 1.45e-82 | 289 |
| MsG0180001297.01.T01 | MTR_2g046550 | 54.930 | 142 | 58 | 1 | 77 | 212 | 103 | 244 | 2.88e-41 | 165 |
| MsG0180001297.01.T01 | MTR_7g110320 | 34.529 | 446 | 264 | 9 | 437 | 862 | 415 | 852 | 4.11e-67 | 242 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180001297.01.T01 | AT1G20980 | 49.010 | 1061 | 438 | 25 | 16 | 1006 | 8 | 1035 | 0.0 | 876 |
| MsG0180001297.01.T01 | AT1G76580 | 47.142 | 1067 | 448 | 25 | 3 | 1006 | 7 | 1020 | 0.0 | 848 |
| MsG0180001297.01.T01 | AT1G76580 | 45.379 | 725 | 296 | 20 | 3 | 665 | 7 | 693 | 7.64e-173 | 521 |
| MsG0180001297.01.T01 | AT1G76580 | 45.367 | 723 | 295 | 20 | 3 | 663 | 7 | 691 | 9.34e-172 | 519 |
| MsG0180001297.01.T01 | AT2G47070 | 33.574 | 554 | 320 | 13 | 469 | 1005 | 229 | 751 | 1.81e-86 | 295 |
| MsG0180001297.01.T01 | AT2G47070 | 58.025 | 81 | 32 | 1 | 132 | 212 | 2 | 80 | 2.12e-20 | 97.8 |
| MsG0180001297.01.T01 | AT3G60030 | 35.599 | 559 | 307 | 17 | 469 | 1005 | 399 | 926 | 2.86e-86 | 298 |
| MsG0180001297.01.T01 | AT3G60030 | 56.934 | 137 | 55 | 3 | 78 | 212 | 97 | 231 | 8.95e-38 | 153 |
| MsG0180001297.01.T01 | AT2G47070 | 33.574 | 554 | 320 | 13 | 469 | 1005 | 358 | 880 | 1.66e-85 | 295 |
| MsG0180001297.01.T01 | AT2G47070 | 43.077 | 195 | 73 | 4 | 46 | 212 | 25 | 209 | 4.23e-37 | 151 |
Find 290 sgRNAs with CRISPR-Local
Find 444 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAATTTCAACACCCAATTC+AGG | 0.301987 | 1:-18988366 | None:intergenic |
| AAACAGCGACCTAGAACACT+AGG | 0.611826 | 1:-18986282 | None:intergenic |
| AAACCCTTGTGGATGAAAAC+GGG | 0.509681 | 1:+18987742 | MsG0180001297.01.T01:CDS |
| AAAGCCTCATCCCTTGACTT+GGG | 0.557399 | 1:-18986607 | None:intergenic |
| AAAGCTGGTTATGCATCTTC+GGG | 0.352663 | 1:+18984997 | MsG0180001297.01.T01:CDS |
| AAAGCTTCTAAGGCTTTGTT+AGG | 0.256564 | 1:+18983144 | MsG0180001297.01.T01:CDS |
| AAATCCTTAGTTAACGGGGC+AGG | 0.442282 | 1:-18984496 | None:intergenic |
| AAATGATGATTCAGATTGCC+AGG | 0.494257 | 1:+18984630 | MsG0180001297.01.T01:CDS |
| AAATTCATCTCCACAACAGG+AGG | 0.631154 | 1:-18984793 | None:intergenic |
| AACAACAGTAGAACCACAAC+AGG | 0.458871 | 1:+18982942 | MsG0180001297.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAAATACAGACAAGAAGTCA+AGG | - | Chr1:18987687-18987706 | None:intergenic | 30.0% | |
| AAAATTTCAACACCCAATTC+AGG | - | Chr1:18988369-18988388 | None:intergenic | 30.0% | |
| AACTTCACTTCTTTGTCTAT+CGG | - | Chr1:18987838-18987857 | None:intergenic | 30.0% | |
| AAGAAAATCCTTAGTTAACG+GGG | - | Chr1:18984503-18984522 | None:intergenic | 30.0% | |
| AATCAACAATCCAATTACAG+GGG | - | Chr1:18985827-18985846 | None:intergenic | 30.0% | |
| AATCCTAAGTTGTAGAGAAA+AGG | - | Chr1:18987615-18987634 | None:intergenic | 30.0% | |
| AATTCAGTAAATCCGAATGA+GGG | - | Chr1:18987111-18987130 | None:intergenic | 30.0% | |
| AGCCAATTGTAAATCTGAAA+AGG | - | Chr1:18985396-18985415 | None:intergenic | 30.0% | |
| AGCTTGTACAAATTCTGAAT+AGG | + | Chr1:18984341-18984360 | MsG0180001297.01.T01:CDS | 30.0% | |
| ATAACTCAGATTCTGATTTC+TGG | + | Chr1:18985637-18985656 | MsG0180001297.01.T01:CDS | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr1 | gene | 18982542 | 18988732 | 18982542 | ID=MsG0180001297.01;Name=MsG0180001297.01 |
| Chr1 | mRNA | 18982542 | 18988732 | 18982542 | ID=MsG0180001297.01.T01;Parent=MsG0180001297.01;Name=MsG0180001297.01.T01;_AED=0.50;_eAED=0.50;_QI=203|1|1|1|1|1|9|581|1006 |
| Chr1 | exon | 18982542 | 18983390 | 18982542 | ID=MsG0180001297.01.T01:exon:6356;Parent=MsG0180001297.01.T01 |
| Chr1 | exon | 18984299 | 18985059 | 18984299 | ID=MsG0180001297.01.T01:exon:6357;Parent=MsG0180001297.01.T01 |
| Chr1 | exon | 18985197 | 18985271 | 18985197 | ID=MsG0180001297.01.T01:exon:6358;Parent=MsG0180001297.01.T01 |
| Chr1 | exon | 18985402 | 18985509 | 18985402 | ID=MsG0180001297.01.T01:exon:6359;Parent=MsG0180001297.01.T01 |
| Chr1 | exon | 18985597 | 18985714 | 18985597 | ID=MsG0180001297.01.T01:exon:6360;Parent=MsG0180001297.01.T01 |
| Chr1 | exon | 18985946 | 18986081 | 18985946 | ID=MsG0180001297.01.T01:exon:6361;Parent=MsG0180001297.01.T01 |
| Chr1 | exon | 18986167 | 18986311 | 18986167 | ID=MsG0180001297.01.T01:exon:6362;Parent=MsG0180001297.01.T01 |
| Chr1 | exon | 18986457 | 18987059 | 18986457 | ID=MsG0180001297.01.T01:exon:6363;Parent=MsG0180001297.01.T01 |
| Chr1 | exon | 18987723 | 18988732 | 18987723 | ID=MsG0180001297.01.T01:exon:6364;Parent=MsG0180001297.01.T01 |
| Chr1 | five_prime_UTR | 18982542 | 18982744 | 18982542 | ID=MsG0180001297.01.T01:five_prime_utr;Parent=MsG0180001297.01.T01 |
| Chr1 | CDS | 18982745 | 18983390 | 18982745 | ID=MsG0180001297.01.T01:cds;Parent=MsG0180001297.01.T01 |
| Chr1 | CDS | 18984299 | 18985059 | 18984299 | ID=MsG0180001297.01.T01:cds;Parent=MsG0180001297.01.T01 |
| Chr1 | CDS | 18985197 | 18985271 | 18985197 | ID=MsG0180001297.01.T01:cds;Parent=MsG0180001297.01.T01 |
| Chr1 | CDS | 18985402 | 18985509 | 18985402 | ID=MsG0180001297.01.T01:cds;Parent=MsG0180001297.01.T01 |
| Chr1 | CDS | 18985597 | 18985714 | 18985597 | ID=MsG0180001297.01.T01:cds;Parent=MsG0180001297.01.T01 |
| Chr1 | CDS | 18985946 | 18986081 | 18985946 | ID=MsG0180001297.01.T01:cds;Parent=MsG0180001297.01.T01 |
| Chr1 | CDS | 18986167 | 18986311 | 18986167 | ID=MsG0180001297.01.T01:cds;Parent=MsG0180001297.01.T01 |
| Chr1 | CDS | 18986457 | 18987059 | 18986457 | ID=MsG0180001297.01.T01:cds;Parent=MsG0180001297.01.T01 |
| Chr1 | CDS | 18987723 | 18988151 | 18987723 | ID=MsG0180001297.01.T01:cds;Parent=MsG0180001297.01.T01 |
| Chr1 | three_prime_UTR | 18988152 | 18988732 | 18988152 | ID=MsG0180001297.01.T01:three_prime_utr;Parent=MsG0180001297.01.T01 |
| Gene Sequence |
>MsG0180001297.01.T01 ATGGAAAAAGTAGCTCCTCCTCTTCTTCCTCTTCATCCTCCTATGTTATCATCACATCAATTTTACGATTCATCAACCACCAAAAAACGTGATCTTTTATCATCATACGACGTCGTTCACATCGGAAACGATAATTGGAACCCTAAAGAATGGAATTGGGACAGTATCCGTTTCATGACAGCAAAATCAACGACGACAACAACAGTAGAACCACAACAGGCCGAAGAATCTCTGAACTTAAATCTTGGAAGCACGGGACAAGTGCGAGCAAACAAACGAATCCGTTCAGGATCTCCAACATCGGCTTCATATCCGATGTGTCAAGTTGATAACTGCAAAGAAGATCTGTCAAAAGCGAAGGATTATCATCGACGTCATAAGGTGTGTGAAGCTCATAGTAAAGCTTCTAAGGCTTTGTTAGGGAATCAAATGCAACGGTTTTGTCAACAGTGTAGTAGGTTTCATCCGTTGGTGGAGTTTGATGAAGGAAAGCGGAGTTGTAGAAGGAGGCTTGCTGGTCATAATCGGCGTCGGAGGAAAACGCAGCCGGATGAAGTGGCGGTTGGAGGTTCGCCGCCGTTGAATCAGGTTGCTGCTAATTTGGAGATTTTTAACTTGTTGACTGCTATTGCTGATGGTTCTCAAGGAAAATTTGAGGAAAGAAGGTCACAAGTTCCAGATAAAGAACAGCTTGTACAAATTCTGAATAGGATCCCTTTACCCGCTGATCTTACTGCAAAATTGCTGGATGTTGGTAATAATTTGAATGCGAAAAATGATAATGTCCAAATGCAAACTTCACCCTCCTATCATCATCGCGATGATCAACCAAACAACACCCCCCCTGCCCCGTTAACTAAGGATTTTCTTGCTGTTCTTTCCACAACTCCATCAACACCTGCTCAAAATGGCGGCAACGGGTCCACAAGTAGTGCAGATCATATGAGAGAAAGAAGCAGTGGCAGTTCTCAGTCTCCAAATGATGATTCAGATTGCCAGGAGGATGTTCGAGTTAAATTGCCGCTGCAACTTTTTGGCTCCTCTCCAGAAAACGACAGCCCATCGAAATTGCCATCTTCGCGAAAGTATTTTTCATCGGAAAGTAGTAATCCTGTGGATGAAAGGTCGCCGTCTTCATCTCCTCCTGTTGTGGAGATGAATTTTGGTCTGCAAGGTGGTATTAGAGGTCTCAATTCTAATGGTATTTTGACTGGATTTGGAGGCCATGCAAATAAGGAAACTAGCCAAAGTCATAGTTGTACTACTATCCCTCTTGATCTCTTTAAAGGGTCAAAATCAAATAACATGATTCAACAATCTAGTTCAGTTCAAAGTGTACCATTTAAAGCTGGTTATGCATCTTCGGGTTCTGATTATTCACCTCCCAGTTTGAATTCAGATAATCAGGATCGTACTGGGAGAATAATGTTTAAATTGTTTGACAAGCATCCTAGCCATTTCCCAGGAACGCTGCGAACACAGATTTACAATTGGCTTTCTACACGACCATCAGACTTGGAGAGTTACATAAGGCCCGGCTGTGTTGTCCTATCAATTTATGCTTCAATGTCTTCTGCTGCTTGGGTGCAATTAGAAGAAAACTTCGTCCAACGTGTTGATTCTTTAATTCATAACTCAGATTCTGATTTCTGGAGAAATGGAAGATTTCTTGTTTATTCTGGCAGTCAGTTAGCATCACACAAAGATGGAAGGATACGCATGTGCAAACCATGGGGAACGTGGAGGTCTCCAGAGTTGATATCAGTGTCTCCTTTGGCAATTGTCGGTGGACAGGAGACTTCTATTTCATTGAAGGGAAGAAACCTCTCTGCTCCTGGCACAAAGATTCATTGCACTGATGCTGACTGTTATACATCATCAGAAGTTATAGGATCAGGAGATCCTGGTATGGTCTATGATGAGATAAAATTGAGTGGTTTTGAAGTTCAGAATACATCTCCTAGTGTTCTAGGTCGCTGTTTCATTGAGGTTGAAAATGGTTTCAAGGGAAACAGTTTTCCAGTGATAATAGCCAATGCATCCATTTGCAAGGAGTTGAGACCGCTTGAGTCTGAATTTGACGAGGATGAAAAGATGTGTGATGCCATTTCAGAGGAGCATGAACATAATTTTGGGAGGCCCAAGTCAAGGGATGAGGCTTTGCACTTTTTGAATGAGCTTGGTTGGCTGTTTCAAAGGGAAAGGTTCTCGAATGTGAACGAGGTTCCAGATTATTCGCTCGACAGGTTCAAATTTGTACTGACGTTTTCTGTGGAAAGAAACTGTTGCATGTTGGTCAAAACCCTTCTGGATATACTGGTTGATAAACACTTCGAAGGAGAAGGGTTGTCAACAGGGTCAGTGGAGATGCTGAAAGCAATCCAACTTCTAAATAGAGCAGTGAAAAGAAAGTGCACAAGCATGGTTGATTTGCTCATTAACTATTCTATAACTAGCAAAAATGACACATCCAAGAAATATGTATTTCCACCGAATCTTGAGGGCCCTGGCGGTATTACTCCCTTGCATTTGGCAGCATCTACAACGGATTCTGAGGGTGTAATTGACTCTTTAACAAATGATCCACAGGAGATTGGGTTGAAGTGCTGGGAAACCCTTGTGGATGAAAACGGGCAGACTCCACATGCCTATGCCATGATGAGAAATAATCACTCTTATAATATGTTGGTTGCCCGTAAACGTTCCGATAGACAAAGAAGTGAAGTTTCAGTTAGAATTGATAATGAGATAGAGCACCCATCACCGGGAATTGAGCTAATGCAAAAGCGAATTAACCAAGTCAAACGAGTTGGGGACTCCTGTTCCAAGTGTGCAATTGCAGAAGTTCGCGCCAAAAGAAGATTTTCCGGTTCACGGAGCTGGCTTCACGGTCCCTTTATTCATTCGATGCTAGCTGTTGCTGCTGTTTGTGTATGTGTTTGTGTGTTATTCCGTGGAACCCCTTATGTAGGCTCAGTTTCTCCCTTCAGGTGGGAAAACTTGAATTATGGCACAATGTAA |
| Protein sequence |
>MsG0180001297.01.T01 MEKVAPPLLPLHPPMLSSHQFYDSSTTKKRDLLSSYDVVHIGNDNWNPKEWNWDSIRFMTAKSTTTTTVEPQQAEESLNLNLGSTGQVRANKRIRSGSPTSASYPMCQVDNCKEDLSKAKDYHRRHKVCEAHSKASKALLGNQMQRFCQQCSRFHPLVEFDEGKRSCRRRLAGHNRRRRKTQPDEVAVGGSPPLNQVAANLEIFNLLTAIADGSQGKFEERRSQVPDKEQLVQILNRIPLPADLTAKLLDVGNNLNAKNDNVQMQTSPSYHHRDDQPNNTPPAPLTKDFLAVLSTTPSTPAQNGGNGSTSSADHMRERSSGSSQSPNDDSDCQEDVRVKLPLQLFGSSPENDSPSKLPSSRKYFSSESSNPVDERSPSSSPPVVEMNFGLQGGIRGLNSNGILTGFGGHANKETSQSHSCTTIPLDLFKGSKSNNMIQQSSSVQSVPFKAGYASSGSDYSPPSLNSDNQDRTGRIMFKLFDKHPSHFPGTLRTQIYNWLSTRPSDLESYIRPGCVVLSIYASMSSAAWVQLEENFVQRVDSLIHNSDSDFWRNGRFLVYSGSQLASHKDGRIRMCKPWGTWRSPELISVSPLAIVGGQETSISLKGRNLSAPGTKIHCTDADCYTSSEVIGSGDPGMVYDEIKLSGFEVQNTSPSVLGRCFIEVENGFKGNSFPVIIANASICKELRPLESEFDEDEKMCDAISEEHEHNFGRPKSRDEALHFLNELGWLFQRERFSNVNEVPDYSLDRFKFVLTFSVERNCCMLVKTLLDILVDKHFEGEGLSTGSVEMLKAIQLLNRAVKRKCTSMVDLLINYSITSKNDTSKKYVFPPNLEGPGGITPLHLAASTTDSEGVIDSLTNDPQEIGLKCWETLVDENGQTPHAYAMMRNNHSYNMLVARKRSDRQRSEVSVRIDNEIEHPSPGIELMQKRINQVKRVGDSCSKCAIAEVRAKRRFSGSRSWLHGPFIHSMLAVAAVCVCVCVLFRGTPYVGSVSPFRWENLNYGTM* |
