Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180001467.01.T01 | KEH40859.1 | 95.51 | 245 | 11 | 0 | 3 | 247 | 26 | 270 | 4.02E-161 | 459 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180001467.01.T01 | P93324 | 51.452 | 241 | 111 | 4 | 10 | 247 | 22 | 259 | 1.59E-74 | 234 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180001467.01.T01 | A0A072VFU6 | 95.510 | 245 | 11 | 0 | 3 | 247 | 26 | 270 | 1.92e-161 | 459 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180001400.01 | MsG0180001467.01 | 0.829307 | 5.538327e-55 | 5.647734e-52 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180001467.01.T01 | MTR_1g036510 | 95.510 | 245 | 11 | 0 | 3 | 247 | 26 | 270 | 4.87e-165 | 459 |
| MsG0180001467.01.T01 | MTR_1g036510 | 95.510 | 245 | 11 | 0 | 3 | 247 | 26 | 270 | 2.31e-163 | 458 |
| MsG0180001467.01.T01 | MTR_1g036510 | 95.397 | 239 | 11 | 0 | 3 | 241 | 26 | 264 | 8.01e-161 | 447 |
| MsG0180001467.01.T01 | MTR_1g036490 | 80.658 | 243 | 47 | 0 | 5 | 247 | 14 | 256 | 8.27e-143 | 405 |
| MsG0180001467.01.T01 | MTR_1g036460 | 77.366 | 243 | 55 | 0 | 5 | 247 | 14 | 256 | 3.81e-138 | 393 |
| MsG0180001467.01.T01 | MTR_1g050220 | 80.000 | 235 | 9 | 1 | 13 | 247 | 5 | 201 | 3.53e-129 | 368 |
| MsG0180001467.01.T01 | MTR_7g012020 | 52.439 | 246 | 109 | 5 | 4 | 247 | 17 | 256 | 1.10e-80 | 245 |
| MsG0180001467.01.T01 | MTR_7g012020 | 52.439 | 246 | 109 | 5 | 4 | 247 | 17 | 256 | 7.22e-80 | 245 |
| MsG0180001467.01.T01 | MTR_7g012020 | 51.653 | 242 | 109 | 5 | 4 | 243 | 17 | 252 | 1.79e-78 | 238 |
| MsG0180001467.01.T01 | MTR_3g021440 | 50.607 | 247 | 116 | 4 | 3 | 247 | 16 | 258 | 1.05e-77 | 239 |
| MsG0180001467.01.T01 | MTR_7g012040 | 50.612 | 245 | 114 | 4 | 4 | 247 | 17 | 255 | 1.92e-77 | 239 |
| MsG0180001467.01.T01 | MTR_7g011900 | 52.675 | 243 | 108 | 5 | 8 | 247 | 19 | 257 | 7.37e-77 | 237 |
| MsG0180001467.01.T01 | MTR_7g012070 | 51.600 | 250 | 114 | 5 | 2 | 247 | 16 | 262 | 3.09e-76 | 236 |
| MsG0180001467.01.T01 | MTR_7g012090 | 52.245 | 245 | 111 | 5 | 8 | 247 | 19 | 262 | 4.76e-76 | 235 |
| MsG0180001467.01.T01 | MTR_1g096310 | 51.037 | 241 | 112 | 4 | 10 | 247 | 22 | 259 | 1.07e-74 | 232 |
| MsG0180001467.01.T01 | MTR_2g055940 | 50.847 | 236 | 109 | 4 | 14 | 247 | 20 | 250 | 6.49e-74 | 229 |
| MsG0180001467.01.T01 | MTR_7g011990 | 49.798 | 247 | 111 | 5 | 4 | 247 | 18 | 254 | 2.36e-73 | 228 |
| MsG0180001467.01.T01 | MTR_3g021430 | 46.923 | 260 | 125 | 7 | 1 | 256 | 1 | 251 | 4.36e-68 | 214 |
| MsG0180001467.01.T01 | MTR_3g092900 | 41.841 | 239 | 133 | 3 | 10 | 247 | 17 | 250 | 4.24e-64 | 204 |
| MsG0180001467.01.T01 | MTR_8g068690 | 39.184 | 245 | 141 | 4 | 10 | 251 | 28 | 267 | 4.27e-57 | 184 |
| MsG0180001467.01.T01 | MTR_8g068690 | 39.184 | 245 | 141 | 4 | 10 | 251 | 28 | 267 | 7.46e-56 | 184 |
| MsG0180001467.01.T01 | MTR_5g098170 | 37.652 | 247 | 145 | 5 | 5 | 247 | 9 | 250 | 7.80e-53 | 176 |
| MsG0180001467.01.T01 | MTR_7g118320 | 39.044 | 251 | 143 | 5 | 2 | 247 | 4 | 249 | 1.96e-52 | 174 |
| MsG0180001467.01.T01 | MTR_7g118300 | 41.365 | 249 | 138 | 5 | 1 | 247 | 20 | 262 | 4.33e-52 | 174 |
| MsG0180001467.01.T01 | MTR_8g068750 | 37.959 | 245 | 144 | 4 | 10 | 251 | 29 | 268 | 6.07e-51 | 171 |
| MsG0180001467.01.T01 | MTR_0591s0010 | 37.344 | 241 | 143 | 4 | 10 | 247 | 29 | 264 | 1.29e-50 | 170 |
| MsG0180001467.01.T01 | MTR_8g068870 | 36.585 | 246 | 148 | 4 | 5 | 247 | 10 | 250 | 8.83e-50 | 167 |
| MsG0180001467.01.T01 | MTR_8g068600 | 38.426 | 216 | 125 | 4 | 35 | 247 | 28 | 238 | 1.25e-45 | 154 |
| MsG0180001467.01.T01 | MTR_6g078850 | 40.329 | 243 | 135 | 5 | 10 | 247 | 33 | 270 | 4.87e-45 | 158 |
| MsG0180001467.01.T01 | MTR_8g068570 | 34.583 | 240 | 124 | 4 | 10 | 247 | 29 | 237 | 6.58e-42 | 146 |
| MsG0180001467.01.T01 | MTR_8g068740 | 34.025 | 241 | 119 | 5 | 10 | 247 | 29 | 232 | 4.24e-35 | 127 |
| MsG0180001467.01.T01 | MTR_3g096050 | 34.274 | 248 | 153 | 5 | 6 | 251 | 9 | 248 | 1.36e-34 | 125 |
| MsG0180001467.01.T01 | MTR_8g068730 | 34.025 | 241 | 119 | 5 | 10 | 247 | 29 | 232 | 1.40e-34 | 127 |
| MsG0180001467.01.T01 | MTR_2g072790 | 34.783 | 253 | 154 | 5 | 1 | 251 | 1 | 244 | 6.30e-34 | 125 |
| MsG0180001467.01.T01 | MTR_3g096050 | 34.274 | 248 | 153 | 5 | 6 | 251 | 9 | 248 | 1.16e-33 | 125 |
| MsG0180001467.01.T01 | MTR_8g024160 | 31.557 | 244 | 120 | 4 | 5 | 247 | 18 | 215 | 4.79e-33 | 123 |
| MsG0180001467.01.T01 | MTR_6g060290 | 39.884 | 173 | 82 | 5 | 4 | 174 | 18 | 170 | 5.26e-30 | 110 |
| MsG0180001467.01.T01 | MTR_8g068620 | 46.809 | 94 | 50 | 0 | 154 | 247 | 2 | 95 | 5.36e-25 | 96.3 |
| MsG0180001467.01.T01 | MTR_3g096070 | 32.258 | 248 | 137 | 7 | 6 | 252 | 9 | 226 | 2.67e-24 | 99.8 |
| MsG0180001467.01.T01 | MTR_0017s0100 | 30.631 | 222 | 141 | 6 | 29 | 247 | 44 | 255 | 2.43e-23 | 97.8 |
| MsG0180001467.01.T01 | MTR_4g038440 | 29.279 | 222 | 144 | 6 | 29 | 247 | 44 | 255 | 4.82e-21 | 89.7 |
| MsG0180001467.01.T01 | MTR_0079s0070 | 29.279 | 222 | 144 | 6 | 29 | 247 | 10 | 221 | 1.16e-20 | 89.7 |
| MsG0180001467.01.T01 | MTR_4g038440 | 29.279 | 222 | 144 | 6 | 29 | 247 | 44 | 255 | 1.76e-20 | 89.7 |
| MsG0180001467.01.T01 | MTR_8g068760 | 36.923 | 130 | 74 | 4 | 12 | 137 | 18 | 143 | 2.60e-19 | 84.3 |
| MsG0180001467.01.T01 | MTR_8g068590 | 33.333 | 126 | 79 | 2 | 5 | 128 | 3 | 125 | 1.19e-18 | 80.5 |
| MsG0180001467.01.T01 | MTR_3g083620 | 38.596 | 114 | 68 | 2 | 135 | 247 | 134 | 246 | 1.49e-18 | 84.3 |
| MsG0180001467.01.T01 | MTR_4g033200 | 26.809 | 235 | 161 | 6 | 21 | 251 | 25 | 252 | 2.02e-14 | 72.4 |
| MsG0180001467.01.T01 | MTR_4g033015 | 26.840 | 231 | 158 | 6 | 21 | 247 | 25 | 248 | 2.66e-14 | 72.0 |
| MsG0180001467.01.T01 | MTR_5g074600 | 25.000 | 252 | 173 | 7 | 6 | 251 | 6 | 247 | 3.57e-14 | 71.6 |
| MsG0180001467.01.T01 | MTR_0794s0020 | 25.561 | 223 | 155 | 6 | 29 | 247 | 33 | 248 | 5.97e-14 | 70.1 |
| MsG0180001467.01.T01 | MTR_4g033150 | 25.561 | 223 | 155 | 6 | 29 | 247 | 33 | 248 | 1.07e-13 | 70.5 |
| MsG0180001467.01.T01 | MTR_4g079160 | 26.190 | 252 | 167 | 6 | 14 | 260 | 13 | 250 | 1.14e-12 | 67.4 |
| MsG0180001467.01.T01 | MTR_1g076940 | 27.523 | 218 | 131 | 8 | 29 | 235 | 33 | 234 | 5.31e-12 | 65.5 |
| MsG0180001467.01.T01 | MTR_7g014570 | 27.935 | 247 | 143 | 8 | 14 | 247 | 14 | 238 | 6.88e-12 | 65.1 |
| MsG0180001467.01.T01 | MTR_4g088200 | 26.549 | 226 | 155 | 5 | 29 | 251 | 33 | 250 | 7.45e-12 | 65.1 |
| MsG0180001467.01.T01 | MTR_7g024500 | 25.342 | 146 | 94 | 2 | 100 | 234 | 81 | 222 | 1.21e-11 | 64.3 |
| MsG0180001467.01.T01 | MTR_4g033170 | 26.068 | 234 | 158 | 6 | 21 | 251 | 25 | 246 | 1.53e-11 | 63.9 |
| MsG0180001467.01.T01 | MTR_4g033085 | 26.068 | 234 | 158 | 6 | 21 | 251 | 25 | 246 | 1.81e-11 | 63.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180001467.01.T01 | AT5G54160 | 43.096 | 239 | 128 | 3 | 10 | 247 | 17 | 248 | 3.51e-63 | 202 |
| MsG0180001467.01.T01 | AT1G77520 | 41.107 | 253 | 134 | 8 | 5 | 247 | 20 | 267 | 1.06e-55 | 183 |
| MsG0180001467.01.T01 | AT1G51990 | 36.820 | 239 | 147 | 3 | 10 | 247 | 13 | 248 | 6.47e-47 | 160 |
| MsG0180001467.01.T01 | AT1G33030 | 39.662 | 237 | 129 | 7 | 16 | 247 | 10 | 237 | 1.24e-46 | 156 |
| MsG0180001467.01.T01 | AT1G33030 | 39.662 | 237 | 129 | 7 | 16 | 247 | 10 | 237 | 1.58e-46 | 157 |
| MsG0180001467.01.T01 | AT1G33030 | 39.662 | 237 | 129 | 7 | 16 | 247 | 10 | 237 | 1.81e-45 | 156 |
| MsG0180001467.01.T01 | AT1G51990 | 35.983 | 239 | 149 | 3 | 10 | 247 | 13 | 248 | 1.03e-44 | 154 |
| MsG0180001467.01.T01 | AT1G63140 | 39.764 | 254 | 132 | 7 | 10 | 251 | 47 | 291 | 1.35e-42 | 147 |
| MsG0180001467.01.T01 | AT1G63140 | 39.764 | 254 | 132 | 7 | 10 | 251 | 27 | 271 | 8.64e-42 | 147 |
| MsG0180001467.01.T01 | AT1G63140 | 39.764 | 254 | 132 | 7 | 10 | 251 | 47 | 291 | 1.09e-41 | 147 |
| MsG0180001467.01.T01 | AT1G77530 | 41.126 | 231 | 123 | 6 | 25 | 247 | 42 | 267 | 2.26e-40 | 143 |
| MsG0180001467.01.T01 | AT1G63140 | 39.344 | 244 | 127 | 7 | 10 | 241 | 47 | 281 | 9.24e-39 | 137 |
| MsG0180001467.01.T01 | AT1G77530 | 40.529 | 227 | 122 | 6 | 25 | 243 | 42 | 263 | 1.76e-38 | 135 |
| MsG0180001467.01.T01 | AT5G53810 | 37.662 | 231 | 130 | 6 | 26 | 247 | 39 | 264 | 9.65e-37 | 134 |
| MsG0180001467.01.T01 | AT1G21130 | 40.083 | 242 | 128 | 9 | 14 | 247 | 27 | 259 | 4.14e-36 | 130 |
| MsG0180001467.01.T01 | AT1G21130 | 40.083 | 242 | 128 | 9 | 14 | 247 | 27 | 259 | 2.49e-35 | 130 |
| MsG0180001467.01.T01 | AT1G21100 | 38.735 | 253 | 138 | 9 | 3 | 247 | 16 | 259 | 2.17e-34 | 127 |
| MsG0180001467.01.T01 | AT1G21110 | 37.945 | 253 | 140 | 9 | 3 | 247 | 16 | 259 | 2.55e-33 | 124 |
| MsG0180001467.01.T01 | AT1G21120 | 37.945 | 253 | 140 | 9 | 3 | 247 | 16 | 259 | 5.56e-33 | 124 |
| MsG0180001467.01.T01 | AT1G21120 | 37.945 | 253 | 140 | 9 | 3 | 247 | 64 | 307 | 3.36e-32 | 122 |
| MsG0180001467.01.T01 | AT3G53140 | 34.241 | 257 | 150 | 9 | 7 | 256 | 7 | 251 | 1.07e-30 | 117 |
| MsG0180001467.01.T01 | AT1G76790 | 35.409 | 257 | 147 | 8 | 1 | 247 | 4 | 251 | 8.70e-30 | 115 |
| MsG0180001467.01.T01 | AT5G37170 | 36.634 | 202 | 90 | 8 | 53 | 247 | 50 | 220 | 3.34e-27 | 107 |
| MsG0180001467.01.T01 | AT5G37170 | 46.465 | 99 | 50 | 2 | 150 | 247 | 24 | 120 | 2.17e-22 | 92.8 |
| MsG0180001467.01.T01 | AT1G62900 | 46.237 | 93 | 50 | 0 | 159 | 251 | 3 | 95 | 9.95e-20 | 84.7 |
| MsG0180001467.01.T01 | AT5G37170 | 54.167 | 72 | 32 | 1 | 177 | 247 | 1 | 72 | 9.29e-19 | 82.0 |
| MsG0180001467.01.T01 | AT4G35150 | 40.625 | 96 | 56 | 1 | 153 | 247 | 108 | 203 | 6.36e-17 | 79.3 |
| MsG0180001467.01.T01 | AT4G35160 | 33.987 | 153 | 93 | 5 | 102 | 247 | 109 | 260 | 8.36e-15 | 73.6 |
Find 59 sgRNAs with CRISPR-Local
Find 135 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACAATCACCTTTCCATATTT+TGG | 0.100611 | 1:+21786165 | None:intergenic |
| TTGAATAAAATTTAATATTT+AGG | 0.118797 | 1:-21785870 | MsG0180001467.01.T01:three_prime_UTR |
| CACAAAGCTTTGCCAAAATA+TGG | 0.182319 | 1:-21786177 | MsG0180001467.01.T01:three_prime_UTR |
| ACTGCAAGCAATATGAAATT+TGG | 0.211032 | 1:+21785997 | None:intergenic |
| ATCACCGGCTTATAAATATT+TGG | 0.220505 | 1:-21787213 | MsG0180001467.01.T01:CDS |
| ATCCATGAACTTTATGAAAA+TGG | 0.232199 | 1:+21787234 | None:intergenic |
| TTGCTTGGGTTTGGGACTTC+TGG | 0.232896 | 1:+21786126 | None:intergenic |
| TCTGAATATCCTTCGATAAA+AGG | 0.235557 | 1:-21787032 | MsG0180001467.01.T01:CDS |
| TCAAGCCTCTCAGCCAATTC+AGG | 0.269845 | 1:+21787756 | None:intergenic |
| ATATGGAAAGGTGATTGTTT+TGG | 0.273476 | 1:-21786160 | MsG0180001467.01.T01:three_prime_UTR |
| TGAAAATGGTCATTGTTGTT+AGG | 0.282307 | 1:+21787248 | None:intergenic |
| GGATAAAGATGGAGGCTCTT+TGG | 0.282636 | 1:-21787616 | MsG0180001467.01.T01:CDS |
| GCCAATTCAGGGTGTTGATT+TGG | 0.300679 | 1:+21787768 | None:intergenic |
| TTTAGCTTGTATGTCACAAT+TGG | 0.318038 | 1:-21786236 | MsG0180001467.01.T01:intron |
| AGGTGTAACTCGTAGCTTAT+TGG | 0.330965 | 1:+21785749 | None:intergenic |
| GATAAAGATGGAGGCTCTTT+GGG | 0.338071 | 1:-21787615 | MsG0180001467.01.T01:CDS |
| AGGATTCTTACACTATATAA+AGG | 0.342110 | 1:-21787122 | MsG0180001467.01.T01:CDS |
| GTCTATGCTCTTTCTCCAAT+TGG | 0.369095 | 1:-21787654 | MsG0180001467.01.T01:CDS |
| GAAAATGGTCATTGTTGTTA+GGG | 0.378056 | 1:+21787249 | None:intergenic |
| ATAGCAGCATTTAGTATTGC+AGG | 0.379466 | 1:+21787879 | None:intergenic |
| TCCAAATCAACACCCTGAAT+TGG | 0.385842 | 1:-21787769 | MsG0180001467.01.T01:CDS |
| AATGCAAAGTATTGACCAAT+TGG | 0.396215 | 1:+21787639 | None:intergenic |
| ATCGTGGATATCATGATGTT+TGG | 0.407390 | 1:-21787572 | MsG0180001467.01.T01:intron |
| TTAGCTTGTATGTCACAATT+GGG | 0.416227 | 1:-21786235 | MsG0180001467.01.T01:intron |
| ACACTATATAAAGGATTTGT+GGG | 0.420495 | 1:-21787113 | MsG0180001467.01.T01:CDS |
| TACACTATATAAAGGATTTG+TGG | 0.423062 | 1:-21787114 | MsG0180001467.01.T01:CDS |
| CTCAGCTATGTCTGGAGTAA+TGG | 0.435264 | 1:-21785969 | MsG0180001467.01.T01:three_prime_UTR |
| AACAAAGCAATGGCTCAATC+TGG | 0.439953 | 1:-21787161 | MsG0180001467.01.T01:CDS |
| CAAAAGTTATGCATGAGCTC+TGG | 0.456555 | 1:-21786025 | MsG0180001467.01.T01:three_prime_UTR |
| TACTTTGCATTGGATAAAGA+TGG | 0.457504 | 1:-21787627 | MsG0180001467.01.T01:CDS |
| ACCATCTTCATTTGTACGAA+TGG | 0.459821 | 1:+21787689 | None:intergenic |
| CTGGTCCATTAGAAATGAAA+AGG | 0.467725 | 1:-21787142 | MsG0180001467.01.T01:CDS |
| AATTGGTCAATACTTTGCAT+TGG | 0.468884 | 1:-21787637 | MsG0180001467.01.T01:CDS |
| CTCACTAACCTTTAGTAAGA+TGG | 0.473210 | 1:+21786744 | None:intergenic |
| GTTGATGTAGGTGGTGGAGT+AGG | 0.476718 | 1:-21787077 | MsG0180001467.01.T01:CDS |
| TTGATGTAGGTGGTGGAGTA+GGG | 0.487539 | 1:-21787076 | MsG0180001467.01.T01:CDS |
| CAAGATGCACCAACTCATCC+AGG | 0.511168 | 1:-21786981 | MsG0180001467.01.T01:intron |
| AGTGATATCTCAGCTATGTC+TGG | 0.511397 | 1:-21785977 | MsG0180001467.01.T01:three_prime_UTR |
| TCATAAAGTTCATGGATCAC+CGG | 0.521104 | 1:-21787228 | MsG0180001467.01.T01:CDS |
| TGGTGATGCCATCTTACTAA+AGG | 0.525689 | 1:-21786752 | MsG0180001467.01.T01:intron |
| CAAGCCTCTCAGCCAATTCA+GGG | 0.537180 | 1:+21787757 | None:intergenic |
| TCCATTCGTACAAATGAAGA+TGG | 0.543565 | 1:-21787690 | MsG0180001467.01.T01:CDS |
| GCAGGGATAGAGCATGTTGA+AGG | 0.553467 | 1:-21786798 | MsG0180001467.01.T01:intron |
| TGAACGTATGTTGACTGTGT+TGG | 0.557787 | 1:-21787736 | MsG0180001467.01.T01:CDS |
| ACACTAGTTGATGTAGGTGG+TGG | 0.561944 | 1:-21787083 | MsG0180001467.01.T01:CDS |
| AGTAAGATGGCATCACCACT+TGG | 0.563966 | 1:+21786757 | None:intergenic |
| TCAAATATTTAACAAAGCAA+TGG | 0.566835 | 1:-21787171 | MsG0180001467.01.T01:CDS |
| TATATATTACCTGGATGAGT+TGG | 0.568273 | 1:+21786972 | None:intergenic |
| TAGCTTGTATGTCACAATTG+GGG | 0.586252 | 1:-21786234 | MsG0180001467.01.T01:three_prime_UTR |
| GGTGCATCTTGAACCACTTG+AGG | 0.590552 | 1:+21786993 | None:intergenic |
| CGATGGAATAATGCTGAGAG+TGG | 0.606484 | 1:+21787591 | None:intergenic |
| AGCTTTGCCAAAATATGGAA+AGG | 0.616745 | 1:-21786172 | MsG0180001467.01.T01:three_prime_UTR |
| GTCTCAACACTAGTTGATGT+AGG | 0.620089 | 1:-21787089 | MsG0180001467.01.T01:CDS |
| AACACCCTGAATTGGCTGAG+AGG | 0.629156 | 1:-21787761 | MsG0180001467.01.T01:CDS |
| TTTGCATTGGATAAAGATGG+AGG | 0.638338 | 1:-21787624 | MsG0180001467.01.T01:CDS |
| CTCTCAGCATTATTCCATCG+TGG | 0.671335 | 1:-21787588 | MsG0180001467.01.T01:CDS |
| TCAACACTAGTTGATGTAGG+TGG | 0.684114 | 1:-21787086 | MsG0180001467.01.T01:CDS |
| AAACATCATGATATCCACGA+TGG | 0.697991 | 1:+21787574 | None:intergenic |
| ATGTTTGAAAGTGTTCCAAG+TGG | 0.736573 | 1:-21786772 | MsG0180001467.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | CATTTTCTAATTATTAATTT+TGG | + | Chr1:21786916-21786935 | None:intergenic | 10.0% |
| !! | CAATAAATTGATCTTTAAAT+TGG | - | Chr1:21787251-21787270 | MsG0180001467.01.T01:CDS | 15.0% |
| !!! | TAACTTTTGAAATTCATTTT+CGG | + | Chr1:21787715-21787734 | None:intergenic | 15.0% |
| !!! | TATTTAAAAACCACATTTTT+TGG | - | Chr1:21787081-21787100 | MsG0180001467.01.T01:CDS | 15.0% |
| !! | AAAATACAATAAATGTGGTT+AGG | + | Chr1:21787484-21787503 | None:intergenic | 20.0% |
| !! | AATTAATAATCCAGTTAATG+TGG | - | Chr1:21787446-21787465 | MsG0180001467.01.T01:intron | 20.0% |
| !! | ACTTATGTATATATATTACC+TGG | + | Chr1:21786791-21786810 | None:intergenic | 20.0% |
| !! | AGAGAAAAATACAATAAATG+TGG | + | Chr1:21787489-21787508 | None:intergenic | 20.0% |
| !! | TCAAATATTTAACAAAGCAA+TGG | - | Chr1:21786580-21786599 | MsG0180001467.01.T01:intron | 20.0% |
| !!! | AAATAAGATTTCTTTTCACA+TGG | + | Chr1:21787066-21787085 | None:intergenic | 20.0% |
| !!! | AATCTCATGTTTTTATACAT+GGG | - | Chr1:21787676-21787695 | MsG0180001467.01.T01:CDS | 20.0% |
| !!! | AATTGGTCTCTTTTTTAAAT+TGG | - | Chr1:21787268-21787287 | MsG0180001467.01.T01:CDS | 20.0% |
| !!! | ATATTCTTCTCTTTTTATAG+TGG | - | Chr1:21786823-21786842 | MsG0180001467.01.T01:intron | 20.0% |
| !!! | ATTAAGTTGTTTCTTTTTTC+AGG | - | Chr1:21786453-21786472 | MsG0180001467.01.T01:intron | 20.0% |
| !!! | CATTGTTATTATGTTTTAGT+AGG | - | Chr1:21785843-21785862 | MsG0180001467.01.T01:three_prime_UTR | 20.0% |
| !!! | GGATTTAGTCAATAATTTTA+AGG | - | Chr1:21787330-21787349 | MsG0180001467.01.T01:intron | 20.0% |
| !!! | TTTATTCAAACTTTTCTTCA+AGG | - | Chr1:21786215-21786234 | MsG0180001467.01.T01:three_prime_UTR | 20.0% |
| !!! | TTTTCCAAATATTTATAAGC+CGG | + | Chr1:21786545-21786564 | None:intergenic | 20.0% |
| !!! | TTTTCTTGTTTTTGTTTTGT+TGG | - | Chr1:21786255-21786274 | MsG0180001467.01.T01:intron | 20.0% |
| !!! | TTTTTTGTCAAATAGTTTAG+TGG | - | Chr1:21787123-21787142 | MsG0180001467.01.T01:CDS | 20.0% |
| ! | AAATAGAGACTAATACTGAT+GGG | - | Chr1:21787414-21787433 | MsG0180001467.01.T01:intron | 25.0% |
| ! | AATCGTATTGAACAATACTT+AGG | + | Chr1:21786436-21786455 | None:intergenic | 25.0% |
| ! | ACACTATATAAAGGATTTGT+GGG | - | Chr1:21786638-21786657 | MsG0180001467.01.T01:intron | 25.0% |
| ! | AGGATTCTTACACTATATAA+AGG | - | Chr1:21786629-21786648 | MsG0180001467.01.T01:intron | 25.0% |
| ! | ATCCATGAACTTTATGAAAA+TGG | + | Chr1:21786520-21786539 | None:intergenic | 25.0% |
| ! | ATTAAGAATCCTAGAAAGAA+TGG | - | Chr1:21786875-21786894 | MsG0180001467.01.T01:intron | 25.0% |
| ! | TAAATAGAGACTAATACTGA+TGG | - | Chr1:21787413-21787432 | MsG0180001467.01.T01:intron | 25.0% |
| ! | TACACTATATAAAGGATTTG+TGG | - | Chr1:21786637-21786656 | MsG0180001467.01.T01:intron | 25.0% |
| ! | TTTACAACTAATTTAGAGAC+CGG | - | Chr1:21787304-21787323 | MsG0180001467.01.T01:intron | 25.0% |
| !! | AAATTAATGCCTTTTATCGA+AGG | + | Chr1:21786731-21786750 | None:intergenic | 25.0% |
| !! | AGAATCCTTTTCATTTCTAA+TGG | + | Chr1:21786617-21786636 | None:intergenic | 25.0% |
| !! | ATATTTTATGCCACATTAAC+TGG | + | Chr1:21787459-21787478 | None:intergenic | 25.0% |
| !! | ATTATTGACTAAATCCATAC+CGG | + | Chr1:21787326-21787345 | None:intergenic | 25.0% |
| !! | TTATTATGGTGTTAACATTC+CGG | - | Chr1:21786362-21786381 | MsG0180001467.01.T01:intron | 25.0% |
| !!! | ATCTCATGTTTTTATACATG+GGG | - | Chr1:21787677-21787696 | MsG0180001467.01.T01:CDS | 25.0% |
| !!! | CAATCTCATGTTTTTATACA+TGG | - | Chr1:21787675-21787694 | MsG0180001467.01.T01:CDS | 25.0% |
| !!! | TAAAAACCACATTTTTTGGT+CGG | - | Chr1:21787085-21787104 | MsG0180001467.01.T01:CDS | 25.0% |
| !!! | TAATTTTGATTTACCTCAAG+TGG | - | Chr1:21786745-21786764 | MsG0180001467.01.T01:intron | 25.0% |
| AATGATACTCCATTCTTTCT+AGG | + | Chr1:21786887-21786906 | None:intergenic | 30.0% | |
| AATTGGTCAATACTTTGCAT+TGG | - | Chr1:21786114-21786133 | MsG0180001467.01.T01:three_prime_UTR | 30.0% | |
| ACTGCAAGCAATATGAAATT+TGG | + | Chr1:21787757-21787776 | None:intergenic | 30.0% | |
| CGATCGAATTTCAAATTACT+AGG | + | Chr1:21786406-21786425 | None:intergenic | 30.0% | |
| TACCAATTGAGTTACACTTA+CGG | - | Chr1:21787226-21787245 | MsG0180001467.01.T01:CDS | 30.0% | |
| TACTTTGCATTGGATAAAGA+TGG | - | Chr1:21786124-21786143 | MsG0180001467.01.T01:three_prime_UTR | 30.0% | |
| TATATATTACCTGGATGAGT+TGG | + | Chr1:21786782-21786801 | None:intergenic | 30.0% | |
| TCTGAATATCCTTCGATAAA+AGG | - | Chr1:21786719-21786738 | MsG0180001467.01.T01:intron | 30.0% | |
| ! | ACAATCACCTTTCCATATTT+TGG | + | Chr1:21787589-21787608 | None:intergenic | 30.0% |
| ! | AGATTACTGAGCATTTTACT+TGG | - | Chr1:21787819-21787838 | MsG0180001467.01.T01:CDS | 30.0% |
| ! | ATATGGAAAGGTGATTGTTT+TGG | - | Chr1:21787591-21787610 | MsG0180001467.01.T01:CDS | 30.0% |
| ! | GAAAATGGTCATTGTTGTTA+GGG | + | Chr1:21786505-21786524 | None:intergenic | 30.0% |
| ! | GACCATTTTCATAAAGTTCA+TGG | - | Chr1:21786515-21786534 | MsG0180001467.01.T01:intron | 30.0% |
| ! | TAATTTGAAATTCGATCGAG+AGG | - | Chr1:21786408-21786427 | MsG0180001467.01.T01:intron | 30.0% |
| ! | TGAAAATGGTCATTGTTGTT+AGG | + | Chr1:21786506-21786525 | None:intergenic | 30.0% |
| !! | AATGCAAAGTATTGACCAAT+TGG | + | Chr1:21786115-21786134 | None:intergenic | 30.0% |
| !! | ATCACCGGCTTATAAATATT+TGG | - | Chr1:21786538-21786557 | MsG0180001467.01.T01:intron | 30.0% |
| !! | TCTCATGTTTTTATACATGG+GGG | - | Chr1:21787678-21787697 | MsG0180001467.01.T01:CDS | 30.0% |
| !! | TTAGCTTGTATGTCACAATT+GGG | - | Chr1:21787516-21787535 | MsG0180001467.01.T01:intron | 30.0% |
| !! | TTTAGCTTGTATGTCACAAT+TGG | - | Chr1:21787515-21787534 | MsG0180001467.01.T01:intron | 30.0% |
| !!! | ATTAGCACTCTAGTTTTCTT+TGG | - | Chr1:21787932-21787951 | MsG0180001467.01.T01:CDS | 30.0% |
| AAACATCATGATATCCACGA+TGG | + | Chr1:21786180-21786199 | None:intergenic | 35.0% | |
| AACTAATTTAGAGACCGGTA+TGG | - | Chr1:21787309-21787328 | MsG0180001467.01.T01:intron | 35.0% | |
| ACCATCTTCATTTGTACGAA+TGG | + | Chr1:21786065-21786084 | None:intergenic | 35.0% | |
| AGATTCACACACATAATGTG+TGG | - | Chr1:21787148-21787167 | MsG0180001467.01.T01:CDS | 35.0% | |
| AGCTTTGCCAAAATATGGAA+AGG | - | Chr1:21787579-21787598 | MsG0180001467.01.T01:CDS | 35.0% | |
| ATAGCAGCATTTAGTATTGC+AGG | + | Chr1:21785875-21785894 | None:intergenic | 35.0% | |
| ATCGTGGATATCATGATGTT+TGG | - | Chr1:21786179-21786198 | MsG0180001467.01.T01:three_prime_UTR | 35.0% | |
| ATTCTACCGACCAAAAAATG+TGG | + | Chr1:21787094-21787113 | None:intergenic | 35.0% | |
| CACAAAGCTTTGCCAAAATA+TGG | - | Chr1:21787574-21787593 | MsG0180001467.01.T01:CDS | 35.0% | |
| CACATTAATCTTTGCTTGCA+GGG | - | Chr1:21786936-21786955 | MsG0180001467.01.T01:intron | 35.0% | |
| CTCACTAACCTTTAGTAAGA+TGG | + | Chr1:21787010-21787029 | None:intergenic | 35.0% | |
| CTGGTCCATTAGAAATGAAA+AGG | - | Chr1:21786609-21786628 | MsG0180001467.01.T01:intron | 35.0% | |
| TACCAAGGATATTATGTGCA+GGG | + | Chr1:21787204-21787223 | None:intergenic | 35.0% | |
| TCATAAAGTTCATGGATCAC+CGG | - | Chr1:21786523-21786542 | MsG0180001467.01.T01:intron | 35.0% | |
| TCCATTCGTACAAATGAAGA+TGG | - | Chr1:21786061-21786080 | MsG0180001467.01.T01:three_prime_UTR | 35.0% | |
| TTCCGTAAGTGTAACTCAAT+TGG | + | Chr1:21787231-21787250 | None:intergenic | 35.0% | |
| TTTGCATTGGATAAAGATGG+AGG | - | Chr1:21786127-21786146 | MsG0180001467.01.T01:three_prime_UTR | 35.0% | |
| ! | AACTCAATTGGTAACTACCA+AGG | + | Chr1:21787219-21787238 | None:intergenic | 35.0% |
| ! | CATGCTTAGAGATTTTGCTT+GGG | + | Chr1:21787642-21787661 | None:intergenic | 35.0% |
| ! | GTTAACATTCCGGCTTTTAA+GGG | - | Chr1:21786372-21786391 | MsG0180001467.01.T01:intron | 35.0% |
| ! | TAGAGATTTTGCTTGGGTTT+GGG | + | Chr1:21787636-21787655 | None:intergenic | 35.0% |
| ! | TAGCTTGTATGTCACAATTG+GGG | - | Chr1:21787517-21787536 | MsG0180001467.01.T01:intron | 35.0% |
| ! | TGTTAACATTCCGGCTTTTA+AGG | - | Chr1:21786371-21786390 | MsG0180001467.01.T01:intron | 35.0% |
| ! | TTAGAGATTTTGCTTGGGTT+TGG | + | Chr1:21787637-21787656 | None:intergenic | 35.0% |
| !! | ATGTTTGAAAGTGTTCCAAG+TGG | - | Chr1:21786979-21786998 | MsG0180001467.01.T01:intron | 35.0% |
| !! | CTCATGTTTTTATACATGGG+GGG | - | Chr1:21787679-21787698 | MsG0180001467.01.T01:CDS | 35.0% |
| !!! | ATCAACTTTTTCAACCTCAG+GGG | + | Chr1:21785807-21785826 | None:intergenic | 35.0% |
| !!! | CATCAACTTTTTCAACCTCA+GGG | + | Chr1:21785808-21785827 | None:intergenic | 35.0% |
| !!! | CATTTTTTGGTCGGTAGAAT+TGG | - | Chr1:21787094-21787113 | MsG0180001467.01.T01:CDS | 35.0% |
| !!! | TCATCAACTTTTTCAACCTC+AGG | + | Chr1:21785809-21785828 | None:intergenic | 35.0% |
| AACAAAGCAATGGCTCAATC+TGG | - | Chr1:21786590-21786609 | MsG0180001467.01.T01:intron | 40.0% | |
| AAGGTTAGTGAGTACATGAG+AGG | - | Chr1:21787018-21787037 | MsG0180001467.01.T01:CDS | 40.0% | |
| AGGTTAGTGAGTACATGAGA+GGG | - | Chr1:21787019-21787038 | MsG0180001467.01.T01:CDS | 40.0% | |
| AGTGATATCTCAGCTATGTC+TGG | - | Chr1:21787774-21787793 | MsG0180001467.01.T01:CDS | 40.0% | |
| CAAAAGTTATGCATGAGCTC+TGG | - | Chr1:21787726-21787745 | MsG0180001467.01.T01:CDS | 40.0% | |
| CTACCAAGGATATTATGTGC+AGG | + | Chr1:21787205-21787224 | None:intergenic | 40.0% | |
| GATAAAGATGGAGGCTCTTT+GGG | - | Chr1:21786136-21786155 | MsG0180001467.01.T01:three_prime_UTR | 40.0% | |
| GATCTTCTTCCCTTAAAAGC+CGG | + | Chr1:21786384-21786403 | None:intergenic | 40.0% | |
| GCACATTAATCTTTGCTTGC+AGG | - | Chr1:21786935-21786954 | MsG0180001467.01.T01:intron | 40.0% | |
| GTCTATGCTCTTTCTCCAAT+TGG | - | Chr1:21786097-21786116 | MsG0180001467.01.T01:three_prime_UTR | 40.0% | |
| TCCAAATCAACACCCTGAAT+TGG | - | Chr1:21785982-21786001 | MsG0180001467.01.T01:three_prime_UTR | 40.0% | |
| ! | AGAGACTAATACTGATGGGT+TGG | - | Chr1:21787418-21787437 | MsG0180001467.01.T01:intron | 40.0% |
| ! | GCATGCTTAGAGATTTTGCT+TGG | + | Chr1:21787643-21787662 | None:intergenic | 40.0% |
| ! | GTCTCAACACTAGTTGATGT+AGG | - | Chr1:21786662-21786681 | MsG0180001467.01.T01:intron | 40.0% |
| ! | TCAACACTAGTTGATGTAGG+TGG | - | Chr1:21786665-21786684 | MsG0180001467.01.T01:intron | 40.0% |
| ! | TGAACGTATGTTGACTGTGT+TGG | - | Chr1:21786015-21786034 | MsG0180001467.01.T01:three_prime_UTR | 40.0% |
| ! | TGGTGATGCCATCTTACTAA+AGG | - | Chr1:21786999-21787018 | MsG0180001467.01.T01:CDS | 40.0% |
| !! | TGTTTTTATACATGGGGGGA+AGG | - | Chr1:21787683-21787702 | MsG0180001467.01.T01:CDS | 40.0% |
| !!! | TCAACTTTTTCAACCTCAGG+GGG | + | Chr1:21785806-21785825 | None:intergenic | 40.0% |
| AAGGATATTATGTGCAGGGC+CGG | + | Chr1:21787200-21787219 | None:intergenic | 45.0% | |
| AGTAAGATGGCATCACCACT+TGG | + | Chr1:21786997-21787016 | None:intergenic | 45.0% | |
| AGTTAAGATGATTCCCCCTG+AGG | - | Chr1:21785790-21785809 | MsG0180001467.01.T01:three_prime_UTR | 45.0% | |
| CGATGGAATAATGCTGAGAG+TGG | + | Chr1:21786163-21786182 | None:intergenic | 45.0% | |
| CTCTCAGCATTATTCCATCG+TGG | - | Chr1:21786163-21786182 | MsG0180001467.01.T01:three_prime_UTR | 45.0% | |
| GGATAAAGATGGAGGCTCTT+TGG | - | Chr1:21786135-21786154 | MsG0180001467.01.T01:three_prime_UTR | 45.0% | |
| ! | ACACTAGTTGATGTAGGTGG+TGG | - | Chr1:21786668-21786687 | MsG0180001467.01.T01:intron | 45.0% |
| ! | CTCAGCTATGTCTGGAGTAA+TGG | - | Chr1:21787782-21787801 | MsG0180001467.01.T01:CDS | 45.0% |
| ! | TGTGTGGAGAAGTGCGAAAT+CGG | - | Chr1:21787164-21787183 | MsG0180001467.01.T01:CDS | 45.0% |
| !! | GCCAATTCAGGGTGTTGATT+TGG | + | Chr1:21785986-21786005 | None:intergenic | 45.0% |
| !! | TTGATGTAGGTGGTGGAGTA+GGG | - | Chr1:21786675-21786694 | MsG0180001467.01.T01:intron | 45.0% |
| !! | TTGAATAAAATTTAATATTT+AGG | - | Chr1:21787881-21787900 | MsG0180001467.01.T01:CDS | 5.0% |
| !!! | TGATTATTTTTATTTTATTA+TGG | - | Chr1:21786348-21786367 | MsG0180001467.01.T01:intron | 5.0% |
| AACACCCTGAATTGGCTGAG+AGG | - | Chr1:21785990-21786009 | MsG0180001467.01.T01:three_prime_UTR | 50.0% | |
| AGGATATTATGTGCAGGGCC+GGG | + | Chr1:21787199-21787218 | None:intergenic | 50.0% | |
| CAAGATGCACCAACTCATCC+AGG | - | Chr1:21786770-21786789 | MsG0180001467.01.T01:CDS | 50.0% | |
| CAAGCCTCTCAGCCAATTCA+GGG | + | Chr1:21785997-21786016 | None:intergenic | 50.0% | |
| CGAAATCGGGGTTTAAACCC+CGG | - | Chr1:21787178-21787197 | MsG0180001467.01.T01:CDS | 50.0% | |
| GCAGGGATAGAGCATGTTGA+AGG | - | Chr1:21786953-21786972 | MsG0180001467.01.T01:intron | 50.0% | |
| GGCCCTGCACATAATATCCT+TGG | - | Chr1:21787199-21787218 | MsG0180001467.01.T01:CDS | 50.0% | |
| GGTGCATCTTGAACCACTTG+AGG | + | Chr1:21786761-21786780 | None:intergenic | 50.0% | |
| TCAAGCCTCTCAGCCAATTC+AGG | + | Chr1:21785998-21786017 | None:intergenic | 50.0% | |
| ! | GTGTGGAGAAGTGCGAAATC+GGG | - | Chr1:21787165-21787184 | MsG0180001467.01.T01:CDS | 50.0% |
| ! | TGTGGAGAAGTGCGAAATCG+GGG | - | Chr1:21787166-21787185 | MsG0180001467.01.T01:CDS | 50.0% |
| ! | TTGCTTGGGTTTGGGACTTC+TGG | + | Chr1:21787628-21787647 | None:intergenic | 50.0% |
| !! | GTTGATGTAGGTGGTGGAGT+AGG | - | Chr1:21786674-21786693 | MsG0180001467.01.T01:intron | 50.0% |
| GGATATTATGTGCAGGGCCG+GGG | + | Chr1:21787198-21787217 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr1 | gene | 21785755 | 21788018 | 21785755 | ID=MsG0180001467.01;Name=MsG0180001467.01 |
| Chr1 | mRNA | 21785755 | 21788018 | 21785755 | ID=MsG0180001467.01.T01;Parent=MsG0180001467.01;Name=MsG0180001467.01.T01;_AED=0.24;_eAED=0.24;_QI=42|1|1|1|0.66|0.5|4|501|260 |
| Chr1 | exon | 21787573 | 21788018 | 21787573 | ID=MsG0180001467.01.T01:exon:13612;Parent=MsG0180001467.01.T01 |
| Chr1 | exon | 21786982 | 21787298 | 21786982 | ID=MsG0180001467.01.T01:exon:13613;Parent=MsG0180001467.01.T01 |
| Chr1 | exon | 21786753 | 21786816 | 21786753 | ID=MsG0180001467.01.T01:exon:13614;Parent=MsG0180001467.01.T01 |
| Chr1 | exon | 21785755 | 21786253 | 21785755 | ID=MsG0180001467.01.T01:exon:13615;Parent=MsG0180001467.01.T01 |
| Chr1 | five_prime_UTR | 21787977 | 21788018 | 21787977 | ID=MsG0180001467.01.T01:five_prime_utr;Parent=MsG0180001467.01.T01 |
| Chr1 | CDS | 21787573 | 21787976 | 21787573 | ID=MsG0180001467.01.T01:cds;Parent=MsG0180001467.01.T01 |
| Chr1 | CDS | 21786982 | 21787298 | 21786982 | ID=MsG0180001467.01.T01:cds;Parent=MsG0180001467.01.T01 |
| Chr1 | CDS | 21786755 | 21786816 | 21786755 | ID=MsG0180001467.01.T01:cds;Parent=MsG0180001467.01.T01 |
| Chr1 | three_prime_UTR | 21786753 | 21786754 | 21786753 | ID=MsG0180001467.01.T01:three_prime_utr;Parent=MsG0180001467.01.T01 |
| Chr1 | three_prime_UTR | 21785755 | 21786253 | 21785755 | ID=MsG0180001467.01.T01:three_prime_utr;Parent=MsG0180001467.01.T01 |
| Gene Sequence |
| Protein sequence |
