Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180001400.01.T01 | XP_013466818.2 | 89.16 | 369 | 12 | 2 | 1 | 341 | 16 | 384 | 0 | 658 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180001400.01.T01 | P93324 | 46.237 | 372 | 157 | 8 | 1 | 341 | 13 | 372 | 1.06E-102 | 309 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180001400.01.T01 | A0A396JUB6 | 89.160 | 369 | 12 | 2 | 1 | 341 | 16 | 384 | 0.0 | 658 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180001393.01 | MsG0180001400.01 | 0.842417 | 2.618777e-58 | 3.968517e-55 |
| MsG0180001400.01 | MsG0180001403.01 | 0.848017 | 8.009996e-60 | 1.450007e-56 |
| MsG0180001400.01 | MsG0180001420.01 | 0.802957 | 4.384839e-49 | 2.176436e-46 |
| MsG0180001400.01 | MsG0180001467.01 | 0.829307 | 5.538327e-55 | 5.647734e-52 |
| MsG0180001400.01 | MsG0180002083.01 | 0.801181 | 1.016404e-48 | 4.822637e-46 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180001400.01.T01 | MTR_1g036510 | 88.889 | 369 | 13 | 2 | 1 | 341 | 16 | 384 | 0.0 | 656 |
| MsG0180001400.01.T01 | MTR_1g036490 | 74.324 | 370 | 66 | 3 | 1 | 341 | 1 | 370 | 0.0 | 550 |
| MsG0180001400.01.T01 | MTR_1g036510 | 96.310 | 271 | 10 | 0 | 1 | 271 | 16 | 286 | 0.0 | 534 |
| MsG0180001400.01.T01 | MTR_1g036460 | 71.892 | 370 | 75 | 3 | 1 | 341 | 1 | 370 | 0.0 | 534 |
| MsG0180001400.01.T01 | MTR_1g036510 | 96.386 | 249 | 9 | 0 | 1 | 249 | 16 | 264 | 1.10e-177 | 493 |
| MsG0180001400.01.T01 | MTR_1g050220 | 76.636 | 321 | 19 | 4 | 21 | 323 | 5 | 287 | 3.90e-167 | 468 |
| MsG0180001400.01.T01 | MTR_7g011900 | 48.066 | 362 | 151 | 8 | 10 | 341 | 16 | 370 | 5.21e-104 | 310 |
| MsG0180001400.01.T01 | MTR_3g021440 | 46.667 | 360 | 158 | 6 | 11 | 341 | 16 | 370 | 7.37e-104 | 310 |
| MsG0180001400.01.T01 | MTR_1g096310 | 45.699 | 372 | 159 | 8 | 1 | 341 | 13 | 372 | 9.17e-103 | 307 |
| MsG0180001400.01.T01 | MTR_7g012040 | 46.240 | 359 | 157 | 7 | 12 | 341 | 17 | 368 | 7.34e-100 | 299 |
| MsG0180001400.01.T01 | MTR_7g012070 | 47.514 | 362 | 154 | 8 | 12 | 341 | 18 | 375 | 1.61e-99 | 298 |
| MsG0180001400.01.T01 | MTR_7g012020 | 46.111 | 360 | 157 | 8 | 12 | 341 | 17 | 369 | 1.87e-99 | 298 |
| MsG0180001400.01.T01 | MTR_2g055940 | 47.429 | 350 | 148 | 7 | 22 | 341 | 20 | 363 | 1.06e-98 | 296 |
| MsG0180001400.01.T01 | MTR_7g012090 | 47.632 | 359 | 153 | 8 | 16 | 341 | 19 | 375 | 1.17e-97 | 294 |
| MsG0180001400.01.T01 | MTR_7g011990 | 45.706 | 361 | 154 | 8 | 12 | 341 | 18 | 367 | 1.59e-96 | 291 |
| MsG0180001400.01.T01 | MTR_3g021430 | 44.413 | 358 | 163 | 7 | 14 | 341 | 6 | 357 | 4.58e-93 | 281 |
| MsG0180001400.01.T01 | MTR_7g012020 | 53.409 | 264 | 115 | 5 | 12 | 273 | 17 | 274 | 3.10e-89 | 270 |
| MsG0180001400.01.T01 | MTR_3g092900 | 37.772 | 368 | 192 | 6 | 4 | 341 | 3 | 363 | 1.10e-84 | 260 |
| MsG0180001400.01.T01 | MTR_7g012020 | 51.240 | 242 | 110 | 5 | 12 | 251 | 17 | 252 | 1.87e-76 | 235 |
| MsG0180001400.01.T01 | MTR_7g118300 | 40.884 | 362 | 171 | 9 | 13 | 341 | 24 | 375 | 2.41e-75 | 237 |
| MsG0180001400.01.T01 | MTR_5g098170 | 36.464 | 362 | 190 | 8 | 13 | 341 | 9 | 363 | 5.08e-72 | 228 |
| MsG0180001400.01.T01 | MTR_8g068690 | 36.517 | 356 | 187 | 7 | 18 | 341 | 28 | 376 | 4.30e-69 | 221 |
| MsG0180001400.01.T01 | MTR_7g118320 | 36.712 | 365 | 190 | 8 | 11 | 341 | 5 | 362 | 4.15e-68 | 218 |
| MsG0180001400.01.T01 | MTR_0591s0010 | 37.079 | 356 | 185 | 7 | 18 | 341 | 29 | 377 | 5.58e-68 | 218 |
| MsG0180001400.01.T01 | MTR_8g068870 | 35.457 | 361 | 194 | 7 | 13 | 341 | 10 | 363 | 4.66e-66 | 213 |
| MsG0180001400.01.T01 | MTR_8g068750 | 35.393 | 356 | 191 | 7 | 18 | 341 | 29 | 377 | 2.87e-65 | 211 |
| MsG0180001400.01.T01 | MTR_8g068690 | 39.615 | 260 | 149 | 4 | 18 | 274 | 28 | 282 | 8.43e-62 | 199 |
| MsG0180001400.01.T01 | MTR_6g078850 | 35.854 | 357 | 188 | 8 | 18 | 340 | 33 | 382 | 6.63e-58 | 195 |
| MsG0180001400.01.T01 | MTR_8g024160 | 32.172 | 373 | 169 | 8 | 2 | 341 | 7 | 328 | 8.49e-53 | 177 |
| MsG0180001400.01.T01 | MTR_8g068570 | 34.268 | 321 | 149 | 6 | 18 | 307 | 29 | 318 | 1.57e-51 | 174 |
| MsG0180001400.01.T01 | MTR_8g068600 | 40.260 | 231 | 130 | 4 | 43 | 270 | 28 | 253 | 2.22e-51 | 172 |
| MsG0180001400.01.T01 | MTR_8g068730 | 32.584 | 356 | 169 | 8 | 18 | 341 | 29 | 345 | 1.18e-50 | 172 |
| MsG0180001400.01.T01 | MTR_3g096050 | 32.500 | 360 | 201 | 8 | 14 | 341 | 9 | 358 | 5.46e-48 | 165 |
| MsG0180001400.01.T01 | MTR_2g072790 | 31.680 | 363 | 199 | 9 | 14 | 341 | 6 | 354 | 9.03e-45 | 157 |
| MsG0180001400.01.T01 | MTR_8g068740 | 35.496 | 262 | 129 | 5 | 18 | 276 | 29 | 253 | 3.87e-43 | 150 |
| MsG0180001400.01.T01 | MTR_3g096050 | 36.782 | 261 | 153 | 7 | 14 | 271 | 9 | 260 | 2.17e-42 | 148 |
| MsG0180001400.01.T01 | MTR_3g096070 | 30.641 | 359 | 186 | 10 | 14 | 341 | 9 | 335 | 2.36e-35 | 131 |
| MsG0180001400.01.T01 | MTR_0017s0100 | 29.762 | 336 | 192 | 10 | 37 | 340 | 44 | 367 | 4.38e-35 | 131 |
| MsG0180001400.01.T01 | MTR_0079s0070 | 29.762 | 336 | 192 | 10 | 37 | 340 | 10 | 333 | 2.24e-34 | 129 |
| MsG0180001400.01.T01 | MTR_4g038440 | 29.762 | 336 | 192 | 10 | 37 | 340 | 44 | 367 | 4.54e-34 | 129 |
| MsG0180001400.01.T01 | MTR_8g068620 | 50.459 | 109 | 54 | 0 | 162 | 270 | 2 | 110 | 3.90e-33 | 119 |
| MsG0180001400.01.T01 | MTR_1g037040 | 54.237 | 118 | 18 | 2 | 218 | 334 | 2 | 84 | 3.07e-30 | 110 |
| MsG0180001400.01.T01 | MTR_4g038440 | 31.513 | 238 | 150 | 6 | 37 | 271 | 44 | 271 | 9.97e-28 | 109 |
| MsG0180001400.01.T01 | MTR_6g060290 | 39.306 | 173 | 83 | 5 | 12 | 182 | 18 | 170 | 1.00e-27 | 107 |
| MsG0180001400.01.T01 | MTR_3g083620 | 31.905 | 210 | 110 | 5 | 143 | 320 | 134 | 342 | 5.34e-26 | 107 |
| MsG0180001400.01.T01 | MTR_7g014570 | 26.479 | 355 | 198 | 9 | 27 | 340 | 19 | 351 | 4.74e-24 | 101 |
| MsG0180001400.01.T01 | MTR_4g079160 | 27.273 | 341 | 201 | 7 | 22 | 326 | 13 | 342 | 1.81e-23 | 99.8 |
| MsG0180001400.01.T01 | MTR_4g088200 | 27.193 | 342 | 197 | 10 | 37 | 340 | 33 | 360 | 2.38e-23 | 99.4 |
| MsG0180001400.01.T01 | MTR_4g033150 | 29.098 | 244 | 162 | 7 | 37 | 276 | 33 | 269 | 5.86e-23 | 98.6 |
| MsG0180001400.01.T01 | MTR_7g024500 | 23.599 | 339 | 191 | 6 | 29 | 325 | 24 | 336 | 6.15e-23 | 98.2 |
| MsG0180001400.01.T01 | MTR_5g074600 | 24.932 | 365 | 224 | 10 | 14 | 340 | 6 | 358 | 7.22e-23 | 98.2 |
| MsG0180001400.01.T01 | MTR_4g033015 | 29.365 | 252 | 167 | 6 | 29 | 276 | 25 | 269 | 9.79e-23 | 97.8 |
| MsG0180001400.01.T01 | MTR_1g076940 | 25.926 | 324 | 196 | 7 | 37 | 325 | 33 | 347 | 1.94e-22 | 97.1 |
| MsG0180001400.01.T01 | MTR_4g033200 | 28.571 | 252 | 169 | 6 | 29 | 276 | 25 | 269 | 1.91e-21 | 94.4 |
| MsG0180001400.01.T01 | MTR_7g024520 | 23.599 | 339 | 191 | 6 | 29 | 325 | 24 | 336 | 3.45e-21 | 93.2 |
| MsG0180001400.01.T01 | MTR_7g014510 | 23.496 | 349 | 197 | 6 | 37 | 340 | 33 | 356 | 5.02e-20 | 90.1 |
| MsG0180001400.01.T01 | MTR_5g074680 | 25.723 | 346 | 211 | 9 | 29 | 340 | 26 | 359 | 1.09e-19 | 89.4 |
| MsG0180001400.01.T01 | MTR_4g088345 | 24.620 | 329 | 195 | 8 | 34 | 325 | 30 | 342 | 1.30e-18 | 85.9 |
| MsG0180001400.01.T01 | MTR_5g074580 | 24.865 | 370 | 224 | 10 | 13 | 340 | 2 | 359 | 1.49e-18 | 85.9 |
| MsG0180001400.01.T01 | MTR_4g033170 | 27.490 | 251 | 167 | 6 | 29 | 276 | 25 | 263 | 2.15e-18 | 85.5 |
| MsG0180001400.01.T01 | MTR_4g033085 | 27.490 | 251 | 167 | 6 | 29 | 276 | 25 | 263 | 2.68e-18 | 85.1 |
| MsG0180001400.01.T01 | MTR_1g037140 | 66.667 | 60 | 19 | 1 | 270 | 328 | 53 | 112 | 6.45e-18 | 79.0 |
| MsG0180001400.01.T01 | MTR_5g074540 | 28.163 | 245 | 136 | 5 | 129 | 340 | 103 | 340 | 6.77e-18 | 84.0 |
| MsG0180001400.01.T01 | MTR_4g088340 | 25.000 | 336 | 195 | 10 | 29 | 325 | 2 | 319 | 8.93e-18 | 83.2 |
| MsG0180001400.01.T01 | MTR_8g068760 | 36.154 | 130 | 75 | 4 | 20 | 145 | 18 | 143 | 1.31e-17 | 81.3 |
| MsG0180001400.01.T01 | MTR_8g068590 | 33.333 | 126 | 79 | 2 | 13 | 136 | 3 | 125 | 1.96e-17 | 78.6 |
| MsG0180001400.01.T01 | MTR_1g076930 | 25.681 | 257 | 170 | 9 | 29 | 279 | 2 | 243 | 1.58e-16 | 78.6 |
| MsG0180001400.01.T01 | MTR_4g088190 | 26.667 | 345 | 198 | 9 | 37 | 340 | 33 | 363 | 2.78e-16 | 79.3 |
| MsG0180001400.01.T01 | MTR_6g060280 | 61.818 | 55 | 16 | 1 | 222 | 271 | 1 | 55 | 3.99e-15 | 70.1 |
| MsG0180001400.01.T01 | MTR_5g074610 | 28.994 | 169 | 87 | 3 | 203 | 340 | 159 | 325 | 1.33e-14 | 73.9 |
| MsG0180001400.01.T01 | MTR_0794s0020 | 25.893 | 224 | 155 | 7 | 37 | 256 | 33 | 249 | 1.10e-13 | 70.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180001400.01.T01 | AT5G54160 | 39.831 | 354 | 176 | 5 | 4 | 327 | 3 | 349 | 3.98e-83 | 256 |
| MsG0180001400.01.T01 | AT1G77520 | 37.752 | 347 | 171 | 11 | 33 | 341 | 42 | 381 | 1.93e-70 | 224 |
| MsG0180001400.01.T01 | AT1G51990 | 34.731 | 334 | 184 | 6 | 18 | 320 | 13 | 343 | 1.78e-60 | 198 |
| MsG0180001400.01.T01 | AT1G51990 | 34.132 | 334 | 186 | 6 | 18 | 320 | 13 | 343 | 2.74e-58 | 192 |
| MsG0180001400.01.T01 | AT1G33030 | 34.561 | 353 | 184 | 11 | 24 | 341 | 10 | 350 | 4.81e-56 | 186 |
| MsG0180001400.01.T01 | AT1G77530 | 37.464 | 347 | 172 | 10 | 33 | 341 | 42 | 381 | 2.87e-55 | 185 |
| MsG0180001400.01.T01 | AT1G63140 | 35.519 | 366 | 183 | 11 | 18 | 341 | 27 | 381 | 1.36e-54 | 183 |
| MsG0180001400.01.T01 | AT1G63140 | 35.519 | 366 | 183 | 11 | 18 | 341 | 47 | 401 | 2.04e-54 | 183 |
| MsG0180001400.01.T01 | AT1G33030 | 39.130 | 253 | 140 | 7 | 24 | 271 | 10 | 253 | 1.71e-49 | 166 |
| MsG0180001400.01.T01 | AT1G33030 | 39.130 | 253 | 140 | 7 | 24 | 271 | 10 | 253 | 2.01e-49 | 167 |
| MsG0180001400.01.T01 | AT5G53810 | 35.159 | 347 | 179 | 12 | 34 | 341 | 39 | 378 | 1.93e-48 | 167 |
| MsG0180001400.01.T01 | AT1G63140 | 39.850 | 266 | 139 | 7 | 18 | 271 | 47 | 303 | 2.18e-48 | 165 |
| MsG0180001400.01.T01 | AT1G21130 | 34.037 | 379 | 198 | 14 | 1 | 341 | 9 | 373 | 4.41e-46 | 161 |
| MsG0180001400.01.T01 | AT1G21100 | 33.773 | 379 | 199 | 14 | 1 | 341 | 9 | 373 | 8.29e-45 | 157 |
| MsG0180001400.01.T01 | AT1G21110 | 33.421 | 380 | 199 | 14 | 1 | 341 | 9 | 373 | 8.62e-44 | 155 |
| MsG0180001400.01.T01 | AT1G76790 | 35.159 | 347 | 176 | 12 | 22 | 328 | 17 | 354 | 1.26e-42 | 151 |
| MsG0180001400.01.T01 | AT1G21120 | 33.158 | 380 | 200 | 14 | 1 | 341 | 9 | 373 | 1.49e-42 | 151 |
| MsG0180001400.01.T01 | AT1G21130 | 38.596 | 285 | 155 | 10 | 1 | 277 | 9 | 281 | 2.40e-42 | 149 |
| MsG0180001400.01.T01 | AT3G53140 | 32.565 | 347 | 188 | 11 | 15 | 324 | 7 | 344 | 9.17e-42 | 149 |
| MsG0180001400.01.T01 | AT1G21120 | 33.158 | 380 | 200 | 14 | 1 | 341 | 57 | 421 | 1.15e-41 | 150 |
| MsG0180001400.01.T01 | AT1G77530 | 40.444 | 225 | 121 | 6 | 33 | 249 | 42 | 261 | 8.34e-37 | 134 |
| MsG0180001400.01.T01 | AT1G63140 | 37.450 | 251 | 136 | 7 | 18 | 256 | 47 | 288 | 1.07e-36 | 134 |
| MsG0180001400.01.T01 | AT5G37170 | 33.019 | 318 | 143 | 11 | 61 | 341 | 50 | 334 | 1.57e-36 | 134 |
| MsG0180001400.01.T01 | AT1G62900 | 36.585 | 205 | 98 | 4 | 167 | 341 | 3 | 205 | 2.13e-34 | 125 |
| MsG0180001400.01.T01 | AT5G37170 | 36.279 | 215 | 102 | 6 | 158 | 341 | 24 | 234 | 7.50e-33 | 122 |
| MsG0180001400.01.T01 | AT5G37170 | 37.766 | 188 | 84 | 5 | 185 | 341 | 1 | 186 | 6.74e-30 | 113 |
| MsG0180001400.01.T01 | AT4G35150 | 30.542 | 203 | 103 | 5 | 161 | 325 | 108 | 310 | 4.87e-27 | 108 |
| MsG0180001400.01.T01 | AT4G35160 | 27.547 | 265 | 140 | 10 | 123 | 341 | 123 | 381 | 3.01e-21 | 94.0 |
Find 60 sgRNAs with CRISPR-Local
Find 93 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACTGCAAGCAATATGAAATT+TGG | 0.211032 | 1:+20576559 | None:intergenic |
| ATCCATGAACTTTATGAAAA+TGG | 0.232199 | 1:+20577139 | None:intergenic |
| TTGCTTGGGTTTGGGACTTC+TGG | 0.232896 | 1:+20576686 | None:intergenic |
| ATCACCGGCTTATAAATATT+TGG | 0.239790 | 1:-20577118 | MsG0180001400.01.T01:CDS |
| TCTGAATATCCTTCAATAAA+AGG | 0.244422 | 1:-20576937 | MsG0180001400.01.T01:CDS |
| CTTACTAAAGGTGATTGTTT+TGG | 0.246682 | 1:-20576720 | MsG0180001400.01.T01:CDS |
| TCAAGCCTCTCAGCCAATTC+AGG | 0.269845 | 1:+20577690 | None:intergenic |
| TGAAAATGGTCATTGTTGTT+AGG | 0.282307 | 1:+20577153 | None:intergenic |
| GGATAAAGATGGAGGCTCTT+TGG | 0.282636 | 1:-20577550 | MsG0180001400.01.T01:CDS |
| GCCAATTCAGGGTGTTGATT+TGG | 0.300679 | 1:+20577702 | None:intergenic |
| TTCTTTCTTACCATCTTCAT+TGG | 0.332460 | 1:+20577614 | None:intergenic |
| GATAAAGATGGAGGCTCTTT+GGG | 0.338071 | 1:-20577549 | MsG0180001400.01.T01:CDS |
| AGGATTCTTACACTATATAA+AGG | 0.342110 | 1:-20577027 | MsG0180001400.01.T01:CDS |
| GAAAATGGTCATTGTTGTTA+GGG | 0.378056 | 1:+20577154 | None:intergenic |
| ATAGCAGCATTTAGTATTGC+AGG | 0.379466 | 1:+20577813 | None:intergenic |
| TCCAAATCAACACCCTGAAT+TGG | 0.385842 | 1:-20577703 | MsG0180001400.01.T01:CDS |
| TACACTATATAAAGGATTTG+AGG | 0.415995 | 1:-20577019 | MsG0180001400.01.T01:CDS |
| GTTTATGCCCTCTCATCAAT+TGG | 0.429611 | 1:-20577588 | MsG0180001400.01.T01:CDS |
| CAAAACTTATGTATGAGCTC+TGG | 0.430401 | 1:-20576587 | MsG0180001400.01.T01:CDS |
| CTCAGCTATGTCTGGAGTAA+TGG | 0.435264 | 1:-20576531 | MsG0180001400.01.T01:CDS |
| ATCGTGGATATCATGATGTT+TGG | 0.437952 | 1:-20577506 | MsG0180001400.01.T01:intron |
| TTCTCCAAATATTTATAAGC+CGG | 0.439608 | 1:+20577114 | None:intergenic |
| AACAAAGCAATGGCTCAATC+TGG | 0.439953 | 1:-20577066 | MsG0180001400.01.T01:CDS |
| GTTGATGTGGGTGGTGAAGT+AGG | 0.440684 | 1:-20576982 | MsG0180001400.01.T01:CDS |
| AACAATCACCTTTAGTAAGA+TGG | 0.452675 | 1:+20576724 | None:intergenic |
| TACTTTGCATTGGATAAAGA+TGG | 0.457504 | 1:-20577561 | MsG0180001400.01.T01:CDS |
| ACACTATATAAAGGATTTGA+GGG | 0.464404 | 1:-20577018 | MsG0180001400.01.T01:CDS |
| CTGGTCCATTAGAAATGAAA+AGG | 0.467725 | 1:-20577047 | MsG0180001400.01.T01:CDS |
| AATTGGTCAATACTTTGCAT+TGG | 0.471367 | 1:-20577571 | MsG0180001400.01.T01:CDS |
| TTGATGTGGGTGGTGAAGTA+GGG | 0.487145 | 1:-20576981 | MsG0180001400.01.T01:CDS |
| GTCTCAACACTGGTTGATGT+GGG | 0.498413 | 1:-20576994 | MsG0180001400.01.T01:CDS |
| ACCATCTTCATTGGTACGAA+TGG | 0.502294 | 1:+20577623 | None:intergenic |
| TGGTGCATCTTGAACCACTT+GGG | 0.504233 | 1:+20576897 | None:intergenic |
| AAACTGAGTTTGTTTGGTGT+TGG | 0.509463 | 1:+20577905 | None:intergenic |
| CAAGATGCACCAACTCATCC+AGG | 0.511168 | 1:-20576886 | MsG0180001400.01.T01:intron |
| AGTGATATCTCAGCTATGTC+TGG | 0.511397 | 1:-20576539 | MsG0180001400.01.T01:CDS |
| TCATAAAGTTCATGGATCAC+CGG | 0.521104 | 1:-20577133 | MsG0180001400.01.T01:CDS |
| CAAGCCTCTCAGCCAATTCA+GGG | 0.537180 | 1:+20577691 | None:intergenic |
| TCCATTCGTACCAATGAAGA+TGG | 0.540221 | 1:-20577624 | MsG0180001400.01.T01:CDS |
| TTGGTGCATCTTGAACCACT+TGG | 0.550736 | 1:+20576896 | None:intergenic |
| GCAGGGATAGAGCATGTTGA+AGG | 0.553467 | 1:-20576779 | MsG0180001400.01.T01:intron |
| TGGTGATGCCATCTTACTAA+AGG | 0.555978 | 1:-20576732 | MsG0180001400.01.T01:CDS |
| GTATTGACCAATTGATGAGA+GGG | 0.556808 | 1:+20577581 | None:intergenic |
| TGAACGTATGTTGACTGTGT+TGG | 0.557787 | 1:-20577670 | MsG0180001400.01.T01:CDS |
| AGTAAGATGGCATCACCACT+TGG | 0.563966 | 1:+20576737 | None:intergenic |
| TCAAATATTTAACAAAGCAA+TGG | 0.566835 | 1:-20577076 | MsG0180001400.01.T01:CDS |
| TATATATTACCTGGATGAGT+TGG | 0.568273 | 1:+20576877 | None:intergenic |
| AGTCTCAACACTGGTTGATG+TGG | 0.571744 | 1:-20576995 | MsG0180001400.01.T01:CDS |
| CGATGGATTAATGCTGAGAG+TGG | 0.600634 | 1:+20577525 | None:intergenic |
| GGTGCATCTTGAACCACTTG+GGG | 0.609131 | 1:+20576898 | None:intergenic |
| AACACCCTGAATTGGCTGAG+AGG | 0.629156 | 1:-20577695 | MsG0180001400.01.T01:CDS |
| TTTGCATTGGATAAAGATGG+AGG | 0.638338 | 1:-20577558 | MsG0180001400.01.T01:CDS |
| AGTATTGACCAATTGATGAG+AGG | 0.639341 | 1:+20577580 | None:intergenic |
| CTCTCAGCATTAATCCATCG+TGG | 0.651626 | 1:-20577522 | MsG0180001400.01.T01:CDS |
| AACATAATAACAACGCGGAG+AGG | 0.666267 | 1:+20577850 | None:intergenic |
| ATTTGAGGGAGTCTCAACAC+TGG | 0.676304 | 1:-20577004 | MsG0180001400.01.T01:CDS |
| AAACATCATGATATCCACGA+TGG | 0.677658 | 1:+20577508 | None:intergenic |
| TCAACACTGGTTGATGTGGG+TGG | 0.685572 | 1:-20576991 | MsG0180001400.01.T01:CDS |
| ATGTTTGAAAGTGTTCCAAG+TGG | 0.742592 | 1:-20576752 | MsG0180001400.01.T01:CDS |
| ACTAAAACATAATAACAACG+CGG | 0.824675 | 1:+20577845 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATTTTCTTTTTTTGTTTTGT+TGG | - | Chr1:20576999-20577018 | MsG0180001400.01.T01:CDS | 15.0% |
| !!! | TAAGTTTTGAAATTCATTTT+CGG | + | Chr1:20577831-20577850 | None:intergenic | 15.0% |
| !!! | TGACTATTTTTACTTTATTA+TGG | - | Chr1:20577121-20577140 | MsG0180001400.01.T01:CDS | 15.0% |
| !! | ACTTATGTATATATATTACC+TGG | + | Chr1:20577564-20577583 | None:intergenic | 20.0% |
| !! | TCAAATATTTAACAAAGCAA+TGG | - | Chr1:20577353-20577372 | MsG0180001400.01.T01:intron | 20.0% |
| !!! | AAATTAATGCCTTTTATTGA+AGG | + | Chr1:20577504-20577523 | None:intergenic | 20.0% |
| !!! | ATTATGTTGTTTCTTTTTTC+AGG | - | Chr1:20577226-20577245 | MsG0180001400.01.T01:intron | 20.0% |
| ! | ACACTATATAAAGGATTTGA+GGG | - | Chr1:20577411-20577430 | MsG0180001400.01.T01:intron | 25.0% |
| ! | ACTAAAACATAATAACAACG+CGG | + | Chr1:20576587-20576606 | None:intergenic | 25.0% |
| ! | AGGATTCTTACACTATATAA+AGG | - | Chr1:20577402-20577421 | MsG0180001400.01.T01:intron | 25.0% |
| ! | ATCCATGAACTTTATGAAAA+TGG | + | Chr1:20577293-20577312 | None:intergenic | 25.0% |
| ! | CACCATGTATTAAAAACATT+TGG | + | Chr1:20577796-20577815 | None:intergenic | 25.0% |
| ! | TACACTATATAAAGGATTTG+AGG | - | Chr1:20577410-20577429 | MsG0180001400.01.T01:intron | 25.0% |
| ! | TCTGAATATCCTTCAATAAA+AGG | - | Chr1:20577492-20577511 | MsG0180001400.01.T01:intron | 25.0% |
| ! | TTCTCCAAATATTTATAAGC+CGG | + | Chr1:20577318-20577337 | None:intergenic | 25.0% |
| !! | AGAATCCTTTTCATTTCTAA+TGG | + | Chr1:20577390-20577409 | None:intergenic | 25.0% |
| !! | CGATCGAATTTAAAATTACT+AGG | + | Chr1:20577179-20577198 | None:intergenic | 25.0% |
| !! | TAATTTTAAATTCGATCGCT+GGG | - | Chr1:20577181-20577200 | MsG0180001400.01.T01:CDS | 25.0% |
| !! | TTATTATGGTGTTAACATTC+CGG | - | Chr1:20577135-20577154 | MsG0180001400.01.T01:CDS | 25.0% |
| !! | TTATTCAAACTGTTTCTTCA+AGG | - | Chr1:20576960-20576979 | MsG0180001400.01.T01:CDS | 25.0% |
| !!! | CAAATGTTTTTAATACATGG+TGG | - | Chr1:20577794-20577813 | MsG0180001400.01.T01:CDS | 25.0% |
| !!! | GACCAAATGTTTTTAATACA+TGG | - | Chr1:20577791-20577810 | MsG0180001400.01.T01:CDS | 25.0% |
| AACAATCACCTTTAGTAAGA+TGG | + | Chr1:20577708-20577727 | None:intergenic | 30.0% | |
| AATTGGTCAATACTTTGCAT+TGG | - | Chr1:20576858-20576877 | MsG0180001400.01.T01:intron | 30.0% | |
| ACTGCAAGCAATATGAAATT+TGG | + | Chr1:20577873-20577892 | None:intergenic | 30.0% | |
| TACTTTGCATTGGATAAAGA+TGG | - | Chr1:20576868-20576887 | MsG0180001400.01.T01:intron | 30.0% | |
| TATATATTACCTGGATGAGT+TGG | + | Chr1:20577555-20577574 | None:intergenic | 30.0% | |
| TTCTTTCTTACCATCTTCAT+TGG | + | Chr1:20576818-20576837 | None:intergenic | 30.0% | |
| ! | CTTACTAAAGGTGATTGTTT+TGG | - | Chr1:20577709-20577728 | MsG0180001400.01.T01:CDS | 30.0% |
| ! | GAAAATGGTCATTGTTGTTA+GGG | + | Chr1:20577278-20577297 | None:intergenic | 30.0% |
| ! | GACCATTTTCATAAAGTTCA+TGG | - | Chr1:20577288-20577307 | MsG0180001400.01.T01:intron | 30.0% |
| ! | GTAATTTTAAATTCGATCGC+TGG | - | Chr1:20577180-20577199 | MsG0180001400.01.T01:CDS | 30.0% |
| ! | TGAAAATGGTCATTGTTGTT+AGG | + | Chr1:20577279-20577298 | None:intergenic | 30.0% |
| !! | AATCGTTTTGAGCAATACTT+AGG | + | Chr1:20577209-20577228 | None:intergenic | 30.0% |
| !! | ATCACCGGCTTATAAATATT+TGG | - | Chr1:20577311-20577330 | MsG0180001400.01.T01:intron | 30.0% |
| !! | GTTTTTAATACATGGTGGAA+AGG | - | Chr1:20577799-20577818 | MsG0180001400.01.T01:CDS | 30.0% |
| !!! | TAATTTTGATTTACCCCAAG+TGG | - | Chr1:20577518-20577537 | MsG0180001400.01.T01:CDS | 30.0% |
| AAACATCATGATATCCACGA+TGG | + | Chr1:20576924-20576943 | None:intergenic | 35.0% | |
| ATAGCAGCATTTAGTATTGC+AGG | + | Chr1:20576619-20576638 | None:intergenic | 35.0% | |
| ATCGTGGATATCATGATGTT+TGG | - | Chr1:20576923-20576942 | MsG0180001400.01.T01:CDS | 35.0% | |
| CAAAACTTATGTATGAGCTC+TGG | - | Chr1:20577842-20577861 | MsG0180001400.01.T01:CDS | 35.0% | |
| CTGGTCCATTAGAAATGAAA+AGG | - | Chr1:20577382-20577401 | MsG0180001400.01.T01:intron | 35.0% | |
| TCATAAAGTTCATGGATCAC+CGG | - | Chr1:20577296-20577315 | MsG0180001400.01.T01:intron | 35.0% | |
| TTTGCATTGGATAAAGATGG+AGG | - | Chr1:20576871-20576890 | MsG0180001400.01.T01:intron | 35.0% | |
| ! | CATGCTTAGAGATTTTGCTT+GGG | + | Chr1:20577760-20577779 | None:intergenic | 35.0% |
| ! | GTATTGACCAATTGATGAGA+GGG | + | Chr1:20576851-20576870 | None:intergenic | 35.0% |
| ! | GTTAACATTCCGGCTTTTAA+CGG | - | Chr1:20577145-20577164 | MsG0180001400.01.T01:CDS | 35.0% |
| ! | TAGAGATTTTGCTTGGGTTT+GGG | + | Chr1:20577754-20577773 | None:intergenic | 35.0% |
| ! | TTAGAGATTTTGCTTGGGTT+TGG | + | Chr1:20577755-20577774 | None:intergenic | 35.0% |
| !! | AAACTGAGTTTGTTTGGTGT+TGG | + | Chr1:20576527-20576546 | None:intergenic | 35.0% |
| !! | AGTATTGACCAATTGATGAG+AGG | + | Chr1:20576852-20576871 | None:intergenic | 35.0% |
| !! | ATGTTTGAAAGTGTTCCAAG+TGG | - | Chr1:20577677-20577696 | MsG0180001400.01.T01:CDS | 35.0% |
| !!! | ATCAACTTTTTCAACCTCAG+GGG | + | Chr1:20576551-20576570 | None:intergenic | 35.0% |
| !!! | CATCAACTTTTTCAACCTCA+GGG | + | Chr1:20576552-20576571 | None:intergenic | 35.0% |
| !!! | TCATCAACTTTTTCAACCTC+AGG | + | Chr1:20576553-20576572 | None:intergenic | 35.0% |
| AACAAAGCAATGGCTCAATC+TGG | - | Chr1:20577363-20577382 | MsG0180001400.01.T01:intron | 40.0% | |
| AACATAATAACAACGCGGAG+AGG | + | Chr1:20576582-20576601 | None:intergenic | 40.0% | |
| ACCATCTTCATTGGTACGAA+TGG | + | Chr1:20576809-20576828 | None:intergenic | 40.0% | |
| AGTGATATCTCAGCTATGTC+TGG | - | Chr1:20577890-20577909 | MsG0180001400.01.T01:CDS | 40.0% | |
| CACGATAATCTTTGCTTGCA+GGG | - | Chr1:20577633-20577652 | MsG0180001400.01.T01:CDS | 40.0% | |
| GATAAAGATGGAGGCTCTTT+GGG | - | Chr1:20576880-20576899 | MsG0180001400.01.T01:intron | 40.0% | |
| GATCTTCTTCCGTTAAAAGC+CGG | + | Chr1:20577157-20577176 | None:intergenic | 40.0% | |
| GTTTATGCCCTCTCATCAAT+TGG | - | Chr1:20576841-20576860 | MsG0180001400.01.T01:intron | 40.0% | |
| TCCAAATCAACACCCTGAAT+TGG | - | Chr1:20576726-20576745 | MsG0180001400.01.T01:CDS | 40.0% | |
| TCCATTCGTACCAATGAAGA+TGG | - | Chr1:20576805-20576824 | MsG0180001400.01.T01:intron | 40.0% | |
| ! | GCATGCTTAGAGATTTTGCT+TGG | + | Chr1:20577761-20577780 | None:intergenic | 40.0% |
| ! | TGAACGTATGTTGACTGTGT+TGG | - | Chr1:20576759-20576778 | MsG0180001400.01.T01:CDS | 40.0% |
| ! | TGGTGATGCCATCTTACTAA+AGG | - | Chr1:20577697-20577716 | MsG0180001400.01.T01:CDS | 40.0% |
| !!! | TCAACTTTTTCAACCTCAGG+GGG | + | Chr1:20576550-20576569 | None:intergenic | 40.0% |
| AGTAAGATGGCATCACCACT+TGG | + | Chr1:20577695-20577714 | None:intergenic | 45.0% | |
| ATTTGAGGGAGTCTCAACAC+TGG | - | Chr1:20577425-20577444 | MsG0180001400.01.T01:intron | 45.0% | |
| CTCTCAGCATTAATCCATCG+TGG | - | Chr1:20576907-20576926 | MsG0180001400.01.T01:CDS | 45.0% | |
| GCACGATAATCTTTGCTTGC+AGG | - | Chr1:20577632-20577651 | MsG0180001400.01.T01:CDS | 45.0% | |
| GGATAAAGATGGAGGCTCTT+TGG | - | Chr1:20576879-20576898 | MsG0180001400.01.T01:intron | 45.0% | |
| TGGTGCATCTTGAACCACTT+GGG | + | Chr1:20577535-20577554 | None:intergenic | 45.0% | |
| TTGATGTGGGTGGTGAAGTA+GGG | - | Chr1:20577448-20577467 | MsG0180001400.01.T01:intron | 45.0% | |
| TTGGTGCATCTTGAACCACT+TGG | + | Chr1:20577536-20577555 | None:intergenic | 45.0% | |
| ! | AGGGGGAAACTGAGTTTGTT+TGG | + | Chr1:20576533-20576552 | None:intergenic | 45.0% |
| ! | AGTCTCAACACTGGTTGATG+TGG | - | Chr1:20577434-20577453 | MsG0180001400.01.T01:intron | 45.0% |
| ! | CTCAGCTATGTCTGGAGTAA+TGG | - | Chr1:20577898-20577917 | MsG0180001400.01.T01:CDS | 45.0% |
| ! | GTCTCAACACTGGTTGATGT+GGG | - | Chr1:20577435-20577454 | MsG0180001400.01.T01:intron | 45.0% |
| !! | CGATGGATTAATGCTGAGAG+TGG | + | Chr1:20576907-20576926 | None:intergenic | 45.0% |
| !! | GCCAATTCAGGGTGTTGATT+TGG | + | Chr1:20576730-20576749 | None:intergenic | 45.0% |
| AACACCCTGAATTGGCTGAG+AGG | - | Chr1:20576734-20576753 | MsG0180001400.01.T01:CDS | 50.0% | |
| ACAAACTCAGTTTCCCCCTG+AGG | - | Chr1:20576534-20576553 | MsG0180001400.01.T01:CDS | 50.0% | |
| CAAGATGCACCAACTCATCC+AGG | - | Chr1:20577543-20577562 | MsG0180001400.01.T01:CDS | 50.0% | |
| CAAGCCTCTCAGCCAATTCA+GGG | + | Chr1:20576741-20576760 | None:intergenic | 50.0% | |
| GCAGGGATAGAGCATGTTGA+AGG | - | Chr1:20577650-20577669 | MsG0180001400.01.T01:CDS | 50.0% | |
| GGTGCATCTTGAACCACTTG+GGG | + | Chr1:20577534-20577553 | None:intergenic | 50.0% | |
| GTTGATGTGGGTGGTGAAGT+AGG | - | Chr1:20577447-20577466 | MsG0180001400.01.T01:intron | 50.0% | |
| TCAAGCCTCTCAGCCAATTC+AGG | + | Chr1:20576742-20576761 | None:intergenic | 50.0% | |
| ! | TCAACACTGGTTGATGTGGG+TGG | - | Chr1:20577438-20577457 | MsG0180001400.01.T01:intron | 50.0% |
| ! | TTGCTTGGGTTTGGGACTTC+TGG | + | Chr1:20577746-20577765 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr1 | gene | 20576517 | 20577934 | 20576517 | ID=MsG0180001400.01;Name=MsG0180001400.01 |
| Chr1 | mRNA | 20576517 | 20577934 | 20576517 | ID=MsG0180001400.01.T01;Parent=MsG0180001400.01;Name=MsG0180001400.01.T01;_AED=0.50;_eAED=0.50;_QI=0|0|0|1|1|1|3|0|341 |
| Chr1 | exon | 20577507 | 20577934 | 20577507 | ID=MsG0180001400.01.T01:exon:26222;Parent=MsG0180001400.01.T01 |
| Chr1 | exon | 20576887 | 20577203 | 20576887 | ID=MsG0180001400.01.T01:exon:26221;Parent=MsG0180001400.01.T01 |
| Chr1 | exon | 20576517 | 20576797 | 20576517 | ID=MsG0180001400.01.T01:exon:26220;Parent=MsG0180001400.01.T01 |
| Chr1 | CDS | 20577507 | 20577934 | 20577507 | ID=MsG0180001400.01.T01:cds;Parent=MsG0180001400.01.T01 |
| Chr1 | CDS | 20576887 | 20577203 | 20576887 | ID=MsG0180001400.01.T01:cds;Parent=MsG0180001400.01.T01 |
| Chr1 | CDS | 20576517 | 20576797 | 20576517 | ID=MsG0180001400.01.T01:cds;Parent=MsG0180001400.01.T01 |
| Gene Sequence |
| Protein sequence |
