AlfalfaGEDB | MsG0180001539.01

M. sativa cultivar ZhongmuNo.1 / MsG0180001539.01

KEGG: K09843
GO: GO:0005506 GO:0016705 GO:0020037 GO:0055114
PFAM: PF00067

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180001539.01.T01	XP_013466883.1	95.429	350	16	0	1	350	1	350	0	648
MsG0180001539.01.T02	XP_013466883.1	95.376	346	16	0	1	346	1	346	0	640
MsG0180001539.01.T03	XP_013466883.1	95.283	318	15	0	1	318	1	318	0	592
MsG0180001539.01.T04	XP_013466883.1	90.763	249	18	1	1	249	1	244	5.67E-155	449

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180001539.01.T01	O81077	63.043	322	116	3	31	350	36	356	7.50E-138	403
MsG0180001539.01.T02	O81077	62.579	318	116	3	31	346	36	352	6.39E-135	395
MsG0180001539.01.T03	O81077	62.759	290	105	3	31	318	36	324	2.26E-120	360
MsG0180001539.01.T04	O81077	62.745	204	74	2	32	233	37	240	1.86E-88	273

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180001539.01.T01	A0A072VFZ6	95.429	350	16	0	1	350	1	350	0.0	648
MsG0180001539.01.T02	A0A072VFZ6	95.376	346	16	0	1	346	1	346	0.0	640
MsG0180001539.01.T03	A0A072VFZ6	95.283	318	15	0	1	318	1	318	0.0	592
MsG0180001539.01.T04	A0A072VFZ6	90.763	249	18	1	1	249	1	244	2.71e-155	449

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0180001539.01	MsG0280010523.01	0.812186	4.811347e-51	3.038495e-48
MsG0180001539.01	MsG0480020433.01	0.815637	8.347896e-52	5.787310e-49
MsG0180001539.01	MsG0680031996.01	0.802420	5.657834e-49	2.770366e-46
MsG0180001539.01	MsG0780040417.01	0.821251	4.458406e-53	3.608236e-50
MsG0180001539.01	MsG0880044626.01	0.821165	4.667413e-53	3.768523e-50
MsG0180001539.01	MsG0880045423.01	0.800310	1.530250e-48	7.102557e-46

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180001539.01.T01	MTR_1g037580	95.429	350	16	0	1	350	1	350	0.0	648
MsG0180001539.01.T01	MTR_5g025610	50.877	342	154	8	11	350	8	337	3.67e-111	330
MsG0180001539.01.T01	MTR_4g086130	52.000	325	152	3	29	350	19	342	8.16e-111	331
MsG0180001539.01.T01	MTR_5g025610	50.877	342	154	8	11	350	8	337	9.07e-111	330
MsG0180001539.01.T01	MTR_5g025610	50.877	342	154	8	11	350	8	337	5.39e-110	329
MsG0180001539.01.T01	MTR_4g086040	49.709	344	160	4	19	350	3	345	1.35e-106	320
MsG0180001539.01.T01	MTR_1g019410	48.116	345	171	5	12	350	1	343	1.79e-105	318
MsG0180001539.01.T01	MTR_8g072260	50.968	310	142	5	44	350	37	339	8.59e-105	315
MsG0180001539.01.T01	MTR_8g072260	50.968	310	142	5	44	350	37	339	1.06e-104	315
MsG0180001539.01.T01	MTR_8g072260	51.133	309	143	4	44	350	37	339	3.83e-104	314

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180001539.01.T01	AT2G29090	63.043	322	116	3	31	350	36	356	7.65e-139	403
MsG0180001539.01.T01	AT2G29090	63.043	322	116	3	31	350	36	356	7.65e-139	403
MsG0180001539.01.T01	AT2G29090	63.066	287	103	3	31	315	36	321	5.50e-123	358
MsG0180001539.01.T01	AT5G45340	51.170	342	158	3	12	350	1	336	2.47e-120	355
MsG0180001539.01.T01	AT4G19230	52.047	342	155	5	12	350	1	336	4.49e-120	355
MsG0180001539.01.T01	AT4G19230	52.047	342	155	5	12	350	1	336	1.15e-119	354
MsG0180001539.01.T01	AT5G45340	51.170	342	158	3	12	350	1	336	1.37e-119	353
MsG0180001539.01.T01	AT3G19270	53.254	338	153	3	14	350	7	340	3.22e-119	352
MsG0180001539.01.T01	AT3G19270	53.254	338	153	3	14	350	7	340	1.78e-118	351
MsG0180001539.01.T01	AT2G29090	61.069	262	99	3	91	350	1	261	8.22e-101	303

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Find 89 sgRNAs with CRISPR-Local

Find 510 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAACTTGCTAGGGAATGTCG+TGG	0.691578	1:+22938934	MsG0180001539.01.T01:CDS
AAAGGGTGAGATGGTGAAGG+AGG	0.557982	1:-22940769	None:intergenic
AAATAAGAAGCAAACTTGCT+AGG	0.490793	1:+22938923	MsG0180001539.01.T01:CDS
AAGAGAATTGTATCATTGCT+TGG	0.456903	1:+22936461	MsG0180001539.01.T01:CDS
AATAAGAAGCAAACTTGCTA+GGG	0.509966	1:+22938924	MsG0180001539.01.T01:CDS
AATATTGATGAAGGCAAGGA+GGG	0.522420	1:+22937005	MsG0180001539.01.T01:intron
AATGAGAGCAGGAATTATGG+TGG	0.662652	1:+22937066	MsG0180001539.01.T01:CDS
AATGGAGATGGAATTCAATA+TGG	0.368593	1:+22944712	MsG0180001539.01.T01:three_prime_UTR
AATGGAGTCCACTCTTGTCC+TGG	0.549474	1:+22940718	MsG0180001539.01.T01:three_prime_UTR
AATTATGGTGGTGGGTTGTT+AGG	0.307932	1:+22937078	MsG0180001539.01.T01:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAAAAGATAGTGCACTTTCA+AGG	+	Chr1:22938486-22938505	MsG0180001539.01.T01:intron	30.0%
AAAACAATGGAGAGAGAAAA+AGG	+	Chr1:22937804-22937823	MsG0180001539.01.T01:intron	30.0%
AAAATTGGCAAGGATGATTA+AGG	-	Chr1:22944398-22944417	None:intergenic	30.0%
AAACATTGAATCACCTATCT+AGG	-	Chr1:22940443-22940462	None:intergenic	30.0%
AAAGTTTGTCTTTGTCTCTA+TGG	-	Chr1:22935573-22935592	None:intergenic	30.0%
AAATTGGCAAGGATGATTAA+GGG	-	Chr1:22944397-22944416	None:intergenic	30.0%
AACATTGAATCACCTATCTA+GGG	-	Chr1:22940442-22940461	None:intergenic	30.0%
AAGAAAATAGTGTGTAGAGT+CGG	+	Chr1:22941806-22941825	MsG0180001539.01.T01:intron	30.0%
AAGAGAATTGTATCATTGCT+TGG	+	Chr1:22936461-22936480	MsG0180001539.01.T01:CDS	30.0%
AAGAGGAGCAATATAATGAA+AGG	-	Chr1:22935514-22935533	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr1	gene	22935431	22944946	22935431	ID=MsG0180001539.01;Name=MsG0180001539.01
Chr1	mRNA	22935431	22944946	22935431	ID=MsG0180001539.01.T01;Parent=MsG0180001539.01;Name=MsG0180001539.01.T01;_AED=0.44;_eAED=0.44;_QI=32\|1\|1\|1\|0\|0\|8\|536\|350
Chr1	exon	22938910	22938999	22938910	ID=MsG0180001539.01.T01:exon:18071;Parent=MsG0180001539.01.T01
Chr1	exon	22940027	22940212	22940027	ID=MsG0180001539.01.T01:exon:18072;Parent=MsG0180001539.01.T01
Chr1	exon	22940682	22940798	22940682	ID=MsG0180001539.01.T01:exon:18073;Parent=MsG0180001539.01.T01
Chr1	exon	22944696	22944946	22944696	ID=MsG0180001539.01.T01:exon:18074;Parent=MsG0180001539.01.T01
Chr1	five_prime_UTR	22935431	22935462	22935431	ID=MsG0180001539.01.T01:five_prime_utr;Parent=MsG0180001539.01.T01
Chr1	CDS	22935463	22935686	22935463	ID=MsG0180001539.01.T01:cds;Parent=MsG0180001539.01.T01
Chr1	CDS	22935966	22936284	22935966	ID=MsG0180001539.01.T01:cds;Parent=MsG0180001539.01.T01
Chr1	CDS	22936396	22936545	22936396	ID=MsG0180001539.01.T01:cds;Parent=MsG0180001539.01.T01
Chr1	CDS	22937018	22937269	22937018	ID=MsG0180001539.01.T01:cds;Parent=MsG0180001539.01.T01
Chr1	CDS	22938910	22938999	22938910	ID=MsG0180001539.01.T01:cds;Parent=MsG0180001539.01.T01
Chr1	CDS	22940027	22940044	22940027	ID=MsG0180001539.01.T01:cds;Parent=MsG0180001539.01.T01
Chr1	three_prime_UTR	22940045	22940212	22940045	ID=MsG0180001539.01.T01:three_prime_utr;Parent=MsG0180001539.01.T01
Chr1	three_prime_UTR	22940682	22940798	22940682	ID=MsG0180001539.01.T01:three_prime_utr;Parent=MsG0180001539.01.T01
Chr1	three_prime_UTR	22944696	22944946	22944696	ID=MsG0180001539.01.T01:three_prime_utr;Parent=MsG0180001539.01.T01
Chr1	mRNA	22935431	22939008	22935431	ID=MsG0180001539.01.T02;Parent=MsG0180001539.01;Name=MsG0180001539.01.T02;_AED=0.46;_eAED=0.46;_QI=32\|1\|0.8\|1\|0\|0\|5\|0\|347
Chr1	exon	22938910	22939008	22938910	ID=MsG0180001539.01.T02:exon:18075;Parent=MsG0180001539.01.T02
Chr1	five_prime_UTR	22935431	22935462	22935431	ID=MsG0180001539.01.T02:five_prime_utr;Parent=MsG0180001539.01.T02
Chr1	CDS	22935463	22935686	22935463	ID=MsG0180001539.01.T02:cds;Parent=MsG0180001539.01.T02
Chr1	CDS	22935966	22936284	22935966	ID=MsG0180001539.01.T02:cds;Parent=MsG0180001539.01.T02
Chr1	CDS	22936396	22936545	22936396	ID=MsG0180001539.01.T02:cds;Parent=MsG0180001539.01.T02
Chr1	CDS	22937018	22937269	22937018	ID=MsG0180001539.01.T02:cds;Parent=MsG0180001539.01.T02
Chr1	CDS	22938910	22939008	22938910	ID=MsG0180001539.01.T02:cds;Parent=MsG0180001539.01.T02
Chr1	mRNA	22935431	22937509	22935431	ID=MsG0180001539.01.T03;Parent=MsG0180001539.01;Name=MsG0180001539.01.T03;_AED=0.46;_eAED=0.50;_QI=32\|1\|0.75\|1\|0\|0\|4\|0\|394
Chr1	exon	22937018	22937509	22937018	ID=MsG0180001539.01.T03:exon:18076;Parent=MsG0180001539.01.T03
Chr1	five_prime_UTR	22935431	22935462	22935431	ID=MsG0180001539.01.T03:five_prime_utr;Parent=MsG0180001539.01.T03
Chr1	CDS	22935463	22935686	22935463	ID=MsG0180001539.01.T03:cds;Parent=MsG0180001539.01.T03
Chr1	CDS	22935966	22936284	22935966	ID=MsG0180001539.01.T03:cds;Parent=MsG0180001539.01.T03
Chr1	CDS	22936396	22936545	22936396	ID=MsG0180001539.01.T03:cds;Parent=MsG0180001539.01.T03
Chr1	CDS	22937018	22937509	22937018	ID=MsG0180001539.01.T03:cds;Parent=MsG0180001539.01.T03
Chr1	mRNA	22935431	22936605	22935431	ID=MsG0180001539.01.T04;Parent=MsG0180001539.01;Name=MsG0180001539.01.T04;_AED=0.47;_eAED=0.48;_QI=32\|1\|0.66\|1\|0\|0\|3\|0\|250
Chr1	exon	22936396	22936605	22936396	ID=MsG0180001539.01.T04:exon:18077;Parent=MsG0180001539.01.T04
Chr1	five_prime_UTR	22935431	22935462	22935431	ID=MsG0180001539.01.T04:five_prime_utr;Parent=MsG0180001539.01.T04
Chr1	CDS	22935463	22935686	22935463	ID=MsG0180001539.01.T04:cds;Parent=MsG0180001539.01.T04
Chr1	CDS	22935966	22936284	22935966	ID=MsG0180001539.01.T04:cds;Parent=MsG0180001539.01.T04
Chr1	CDS	22936396	22936605	22936396	ID=MsG0180001539.01.T04:cds;Parent=MsG0180001539.01.T04
Chr1	mRNA	22940092	22940196	22940092	ID=MsG0180001539.01.T05;Parent=MsG0180001539.01;Name=MsG0180001539.01.T05;_AED=0.50;_eAED=0.50;_QI=0\|-1\|0\|1\|-1\|0\|1\|0\|34
Chr1	exon	22940092	22940196	22940092	ID=MsG0180001539.01.T05:exon:18078;Parent=MsG0180001539.01.T05
Chr1	CDS	22940092	22940196	22940092	ID=MsG0180001539.01.T05:cds;Parent=MsG0180001539.01.T05

Gene Sequence

>MsG0180001539.01.T05

ATGTGGAGCTACAAGGTTACTCCATTCCAAAAGGTTGGAAAGTCCTTCCACTCTTTAGAAGTATTCATCATTCACCTCATTTCTTCCTCAACCACACAAGTTTGA

>MsG0180001539.01.T03

ATGCAACTCTTCACATCACTTACCGATTCTTGTTTAGATTTGTCTCTCCCTTTCATTATATTGCTCCTCTTTTTTACTATCCTTCCCTTCCTTCAATGGTGGAAACACCATAGAGACAAAGACAAACTTTTACCCCCTGGTTCAATGGGTTGGCCTTACTTTGGAGAGACCCTCAAACTCTACACTCAAAATCCAAATTCCTTCTTCTCCACAAGGCAGAAAAGGTATGGAGATATATTCAAGACACACATACTAGGCTGTCCTTGTGTGATGATATCAAGTCCTGAGGCAGCTAGAGCAGTGCTTGTGACACAAGCACATCTTTTTAAACCAACATACCCTCCAAGCAAAGAGAAGATGGTAGGACCAGAAGCTTTGTTCTTTCAACAAGGTTCTTATCACTCCATGCTCAAGAAGCTAGTTCAAGCCTCTTTTTTACCTTCCACACTTAAAAACTCTGTCTCTCATGTGGACCAAATTGTACAAAAATTGGTTCCAACTTGGGCTAATAGAACCATCAACACCTTGCAAGAGATGAAAAAGTATGCTTTTGAAGTGGCTGCAAATTCAGCTTTTGGTGAAATAAAAGAGATGGAAATGGAAGAAATAAGAGAATTGTATCATTGCTTGGAGAAGGGCTACAATTCTTATCCTTTGAAGCTCCATGGAACTTCTTATTGGAAGGCATTGAAGGCAAGGAGGGTTTTGAATGAGAGTATAAGGAGGATAATAGAAAGAAGAAATGAGAGCAGGAATTATGGTGGTGGGTTGTTAGGAATTCTGTTGAGAGGCAAAGGTGATGAGAAGATGTACCAACTAACTGATTCTCAGGTGGCTGATAATATCATTGGTGTCATCTTTGCTGCACATGATACCACAGCTAGTGTTCTAACATGGGTTCTAAAGTACTTGCATGACAATGTCAATCTCTTGGAAACTGTCACAGTAATGCAACATCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTTGCATATTTTGA

>MsG0180001539.01.T01

ATGCAACTCTTCACATCACTTACCGATTCTTGTTTAGATTTGTCTCTCCCTTTCATTATATTGCTCCTCTTTTTTACTATCCTTCCCTTCCTTCAATGGTGGAAACACCATAGAGACAAAGACAAACTTTTACCCCCTGGTTCAATGGGTTGGCCTTACTTTGGAGAGACCCTCAAACTCTACACTCAAAATCCAAATTCCTTCTTCTCCACAAGGCAGAAAAGGTATGGAGATATATTCAAGACACACATACTAGGCTGTCCTTGTGTGATGATATCAAGTCCTGAGGCAGCTAGAGCAGTGCTTGTGACACAAGCACATCTTTTTAAACCAACATACCCTCCAAGCAAAGAGAAGATGGTAGGACCAGAAGCTTTGTTCTTTCAACAAGGTTCTTATCACTCCATGCTCAAGAAGCTAGTTCAAGCCTCTTTTTTACCTTCCACACTTAAAAACTCTGTCTCTCATGTGGACCAAATTGTACAAAAATTGGTTCCAACTTGGGCTAATAGAACCATCAACACCTTGCAAGAGATGAAAAAGTATGCTTTTGAAGTGGCTGCAAATTCAGCTTTTGGTGAAATAAAAGAGATGGAAATGGAAGAAATAAGAGAATTGTATCATTGCTTGGAGAAGGGCTACAATTCTTATCCTTTGAAGCTCCATGGAACTTCTTATTGGAAGGCATTGAAGGCAAGGAGGGTTTTGAATGAGAGTATAAGGAGGATAATAGAAAGAAGAAATGAGAGCAGGAATTATGGTGGTGGGTTGTTAGGAATTCTGTTGAGAGGCAAAGGTGATGAGAAGATGTACCAACTAACTGATTCTCAGGTGGCTGATAATATCATTGGTGTCATCTTTGCTGCACATGATACCACAGCTAGTGTTCTAACATGGGTTCTAAAGTACTTGCATGACAATGTCAATCTCTTGGAAACTGTCACAAAAGAACAAGAAGAAATAAGAAGCAAACTTGCTAGGGAATGTCGTGGACTTTCATGGGAGGATACCAGGCAGATGCCCTTCACAAGCAGGGTAATCCAAGAGACATAG

>MsG0180001539.01.T02

ATGCAACTCTTCACATCACTTACCGATTCTTGTTTAGATTTGTCTCTCCCTTTCATTATATTGCTCCTCTTTTTTACTATCCTTCCCTTCCTTCAATGGTGGAAACACCATAGAGACAAAGACAAACTTTTACCCCCTGGTTCAATGGGTTGGCCTTACTTTGGAGAGACCCTCAAACTCTACACTCAAAATCCAAATTCCTTCTTCTCCACAAGGCAGAAAAGGTATGGAGATATATTCAAGACACACATACTAGGCTGTCCTTGTGTGATGATATCAAGTCCTGAGGCAGCTAGAGCAGTGCTTGTGACACAAGCACATCTTTTTAAACCAACATACCCTCCAAGCAAAGAGAAGATGGTAGGACCAGAAGCTTTGTTCTTTCAACAAGGTTCTTATCACTCCATGCTCAAGAAGCTAGTTCAAGCCTCTTTTTTACCTTCCACACTTAAAAACTCTGTCTCTCATGTGGACCAAATTGTACAAAAATTGGTTCCAACTTGGGCTAATAGAACCATCAACACCTTGCAAGAGATGAAAAAGTATGCTTTTGAAGTGGCTGCAAATTCAGCTTTTGGTGAAATAAAAGAGATGGAAATGGAAGAAATAAGAGAATTGTATCATTGCTTGGAGAAGGGCTACAATTCTTATCCTTTGAAGCTCCATGGAACTTCTTATTGGAAGGCATTGAAGGCAAGGAGGGTTTTGAATGAGAGTATAAGGAGGATAATAGAAAGAAGAAATGAGAGCAGGAATTATGGTGGTGGGTTGTTAGGAATTCTGTTGAGAGGCAAAGGTGATGAGAAGATGTACCAACTAACTGATTCTCAGGTGGCTGATAATATCATTGGTGTCATCTTTGCTGCACATGATACCACAGCTAGTGTTCTAACATGGGTTCTAAAGTACTTGCATGACAATGTCAATCTCTTGGAAACTGTCACAAAAGAACAAGAAGAAATAAGAAGCAAACTTGCTAGGGAATGTCGTGGACTTTCATGGGAGGATACCAGGCAGATGCCCTTCACAAGCAGGGTAAAATAA

>MsG0180001539.01.T04

Protein sequence

>MsG0180001539.01.T05

MWSYKVTPFQKVGKSFHSLEVFIIHLISSSTTQV*

>MsG0180001539.01.T03

MQLFTSLTDSCLDLSLPFIILLLFFTILPFLQWWKHHRDKDKLLPPGSMGWPYFGETLKLYTQNPNSFFSTRQKRYGDIFKTHILGCPCVMISSPEAARAVLVTQAHLFKPTYPPSKEKMVGPEALFFQQGSYHSMLKKLVQASFLPSTLKNSVSHVDQIVQKLVPTWANRTINTLQEMKKYAFEVAANSAFGEIKEMEMEEIRELYHCLEKGYNSYPLKLHGTSYWKALKARRVLNESIRRIIERRNESRNYGGGLLGILLRGKGDEKMYQLTDSQVADNIIGVIFAAHDTTASVLTWVLKYLHDNVNLLETVTVMQHLSLSLSLSLSLSLSLSLSLSLSLSLSLSLSLSLSLSLSLSLSLSLSLSLSLSLSLSLSLSLSLSLSLSLSLSCIF*

>MsG0180001539.01.T01

>MsG0180001539.01.T02

>MsG0180001539.01.T04