Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180001758.01.T01 | KEH16002.1 | 78.182 | 55 | 12 | 0 | 36 | 90 | 14 | 68 | 1.52E-21 | 90.5 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180001758.01.T01 | A0A072TF53 | 78.182 | 55 | 12 | 0 | 36 | 90 | 14 | 68 | 7.25e-22 | 90.5 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180001758.01 | MsG0580024908.01 | 0.839942 | 1.171233e-57 | 1.642944e-54 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180001758.01.T01 | MTR_0389s0030 | 78.182 | 55 | 12 | 0 | 36 | 90 | 14 | 68 | 1.84e-25 | 90.5 |
| MsG0180001758.01.T01 | MTR_5g029155 | 70.909 | 55 | 16 | 0 | 33 | 87 | 15 | 69 | 4.82e-22 | 82.4 |
| MsG0180001758.01.T01 | MTR_0083s0050 | 73.585 | 53 | 12 | 1 | 36 | 86 | 1 | 53 | 2.85e-21 | 80.1 |
| MsG0180001758.01.T01 | MTR_3g048420 | 65.306 | 49 | 17 | 0 | 1 | 49 | 1 | 49 | 5.76e-17 | 70.1 |
| MsG0180001758.01.T01 | MTR_3g090210 | 66.667 | 45 | 15 | 0 | 42 | 86 | 60 | 104 | 5.30e-15 | 65.9 |
| MsG0180001758.01.T01 | MTR_8g461950 | 45.455 | 77 | 40 | 1 | 4 | 78 | 13 | 89 | 5.00e-12 | 57.0 |
| MsG0180001758.01.T01 | MTR_5g053355 | 96.154 | 26 | 1 | 0 | 1 | 26 | 1 | 26 | 8.50e-12 | 57.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 18 sgRNAs with CRISPR-Local
Find 23 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CAATGAGATTTCTCGCCAAC+TGG | 0.291788 | 1:+26463297 | MsG0180001758.01.T01:CDS |
| CCTGTGTCCGAGGCTGAAAA+AGG | 0.322209 | 1:+26463257 | MsG0180001758.01.T01:CDS |
| TGTACTCTCTCTCGACTTTG+AGG | 0.423140 | 1:+26463232 | MsG0180001758.01.T01:CDS |
| TACAGGGTTCAAGTGTTCAA+TGG | 0.443078 | 1:-26463213 | None:intergenic |
| ACAGGGTTCAAGTGTTCAAT+GGG | 0.460097 | 1:-26463212 | None:intergenic |
| CTTGATATTTGCAGCTTCGA+TGG | 0.502344 | 1:-26463330 | None:intergenic |
| CAATATCCTTGCAAGGCAGA+TGG | 0.537688 | 1:-26463405 | None:intergenic |
| GGACTTTCATACACAAAGAC+TGG | 0.547857 | 1:+26463370 | MsG0180001758.01.T01:CDS |
| CAAGAAAGATCGTTCTGAAG+AGG | 0.553652 | 1:+26463349 | MsG0180001758.01.T01:CDS |
| TCAAAGTCGAGAGAGAGTAC+AGG | 0.562304 | 1:-26463230 | None:intergenic |
| ATGCTTCCATCTGCCTTGCA+AGG | 0.565588 | 1:+26463399 | MsG0180001758.01.T01:CDS |
| CTTTGAGGTCCCTGTGTCCG+AGG | 0.568246 | 1:+26463247 | MsG0180001758.01.T01:CDS |
| CAAAGTCGAGAGAGAGTACA+GGG | 0.580265 | 1:-26463229 | None:intergenic |
| AATGAGATTTCTCGCCAACT+GGG | 0.588102 | 1:+26463298 | MsG0180001758.01.T01:CDS |
| CAGTTGGCGAGAAATCTCAT+TGG | 0.597895 | 1:-26463296 | None:intergenic |
| TAAAGTACAATATCCTTGCA+AGG | 0.598425 | 1:-26463412 | None:intergenic |
| GATGGCTAAGATGCCCCAGT+TGG | 0.644539 | 1:-26463312 | None:intergenic |
| ATGAGATTTCTCGCCAACTG+GGG | 0.651552 | 1:+26463299 | MsG0180001758.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| TAAAGTACAATATCCTTGCA+AGG | - | Chr1:26463415-26463434 | None:intergenic | 30.0% | |
| AATGAGATTTCTCGCCAACT+GGG | + | Chr1:26463298-26463317 | MsG0180001758.01.T01:CDS | 40.0% | |
| ACAGGGTTCAAGTGTTCAAT+GGG | - | Chr1:26463215-26463234 | None:intergenic | 40.0% | |
| CTTGATATTTGCAGCTTCGA+TGG | - | Chr1:26463333-26463352 | None:intergenic | 40.0% | |
| GGACTTTCATACACAAAGAC+TGG | + | Chr1:26463370-26463389 | MsG0180001758.01.T01:CDS | 40.0% | |
| TACAGGGTTCAAGTGTTCAA+TGG | - | Chr1:26463216-26463235 | None:intergenic | 40.0% | |
| !! | CAAGAAAGATCGTTCTGAAG+AGG | + | Chr1:26463349-26463368 | MsG0180001758.01.T01:CDS | 40.0% |
| ATGAGATTTCTCGCCAACTG+GGG | + | Chr1:26463299-26463318 | MsG0180001758.01.T01:CDS | 45.0% | |
| CAATATCCTTGCAAGGCAGA+TGG | - | Chr1:26463408-26463427 | None:intergenic | 45.0% | |
| CAATGAGATTTCTCGCCAAC+TGG | + | Chr1:26463297-26463316 | MsG0180001758.01.T01:CDS | 45.0% | |
| CAGTTGGCGAGAAATCTCAT+TGG | - | Chr1:26463299-26463318 | None:intergenic | 45.0% | |
| TGTACTCTCTCTCGACTTTG+AGG | + | Chr1:26463232-26463251 | MsG0180001758.01.T01:CDS | 45.0% | |
| ! | CAAAGTCGAGAGAGAGTACA+GGG | - | Chr1:26463232-26463251 | None:intergenic | 45.0% |
| ! | TCAAAGTCGAGAGAGAGTAC+AGG | - | Chr1:26463233-26463252 | None:intergenic | 45.0% |
| ATGCTTCCATCTGCCTTGCA+AGG | + | Chr1:26463399-26463418 | MsG0180001758.01.T01:CDS | 50.0% | |
| CTGTGTCCGAGGCTGAAAAA+GGG | + | Chr1:26463258-26463277 | MsG0180001758.01.T01:CDS | 50.0% | |
| TGTGTCCGAGGCTGAAAAAG+GGG | + | Chr1:26463259-26463278 | MsG0180001758.01.T01:CDS | 50.0% | |
| !!! | CATCACCCCTTTTTCAGCCT+CGG | - | Chr1:26463267-26463286 | None:intergenic | 50.0% |
| !!! | CTTTTTCAGCCTCGGACACA+GGG | - | Chr1:26463259-26463278 | None:intergenic | 50.0% |
| CCTGTGTCCGAGGCTGAAAA+AGG | + | Chr1:26463257-26463276 | MsG0180001758.01.T01:CDS | 55.0% | |
| GATGGCTAAGATGCCCCAGT+TGG | - | Chr1:26463315-26463334 | None:intergenic | 55.0% | |
| !!! | CCTTTTTCAGCCTCGGACAC+AGG | - | Chr1:26463260-26463279 | None:intergenic | 55.0% |
| !! | CTTTGAGGTCCCTGTGTCCG+AGG | + | Chr1:26463247-26463266 | MsG0180001758.01.T01:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr1 | gene | 26463176 | 26463448 | 26463176 | ID=MsG0180001758.01;Name=MsG0180001758.01 |
| Chr1 | mRNA | 26463176 | 26463448 | 26463176 | ID=MsG0180001758.01.T01;Parent=MsG0180001758.01;Name=MsG0180001758.01.T01;_AED=0.46;_eAED=0.46;_QI=0|-1|0|1|-1|1|1|0|90 |
| Chr1 | exon | 26463176 | 26463448 | 26463176 | ID=MsG0180001758.01.T01:exon:18538;Parent=MsG0180001758.01.T01 |
| Chr1 | CDS | 26463176 | 26463448 | 26463176 | ID=MsG0180001758.01.T01:cds;Parent=MsG0180001758.01.T01 |
| Gene Sequence |
| Protein sequence |
