AlfalfaGEDB | MsG0180001807.01

M. sativa cultivar ZhongmuNo.1 / MsG0180001807.01

KEGG: -
GO: -
PFAM: PF04535

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180001807.01.T01	XP_003590058.1	99.342	152	1	0	1	152	1	152	8.00E-103	301
MsG0180001807.01.T02	XP_003590058.1	92.105	152	1	1	1	141	1	152	3.11E-91	271
MsG0180001807.01.T03	XP_024637098.1	99.265	136	1	0	1	136	1	136	1.09E-90	269
MsG0180001807.01.T04	XP_024637098.1	97.059	136	0	1	1	132	1	136	1.90E-85	255
MsG0180001807.01.T05	XP_003590058.1	99.029	103	1	0	1	103	50	152	3.47E-65	203
MsG0180001807.01.T06	XP_024637098.1	100	41	0	0	1	41	96	136	1.91E-20	87.4

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180001807.01.T01	Q8L7R5	62.914	151	56	0	1	151	1	151	5.55E-66	200
MsG0180001807.01.T02	Q8L7R5	56.291	151	55	1	1	140	1	151	8.16E-55	172
MsG0180001807.01.T03	Q8L7R5	54.305	151	53	1	1	135	1	151	2.00E-51	163
MsG0180001807.01.T04	Q8L7R5	52.318	151	52	1	1	131	1	151	1.57E-47	153
MsG0180001807.01.T05	Q8L7R5	67.647	102	33	0	1	102	50	151	1.28E-45	147

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180001807.01.T01	G7I5E9	99.342	152	1	0	1	152	1	152	3.82e-103	301
MsG0180001807.01.T02	G7I5E9	92.105	152	1	1	1	141	1	152	1.49e-91	271
MsG0180001807.01.T03	G7I5E9	88.816	152	1	1	1	136	1	152	1.32e-86	258
MsG0180001807.01.T04	G7I5E9	86.842	152	0	1	1	132	1	152	4.99e-83	249
MsG0180001807.01.T05	G7I5E9	99.029	103	1	0	1	103	50	152	1.66e-65	203
MsG0180001807.01.T06	G7I5E9	100.000	41	0	0	1	41	112	152	1.42e-20	87.0

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TFs/TRs:

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Gene ID	Type	Classification
MsG0180001807.01.T05	TF	C3H

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Protein Kinases:

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Gene ID	Type	Classification
No data available in table

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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
No data available in table

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PPI

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Gene1	Gene2	Type
MsG0180001807.01	MsG0580024876.01	PPI
MsG0180001807.01	MsG0580024876.01	PPI
MsG0180001807.01	MsG0580024876.01	PPI
MsG0580024876.01	MsG0180001807.01	PPI

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180001807.01.T01	MTR_1g043450	99.342	152	1	0	1	152	1	152	9.69e-107	301
MsG0180001807.01.T01	MTR_1g073950	70.395	152	45	0	1	152	1	152	1.28e-77	227
MsG0180001807.01.T01	MTR_1g074040	71.560	109	31	0	44	152	43	151	9.44e-56	172
MsG0180001807.01.T01	MTR_4g021340	51.634	153	72	1	1	151	2	154	1.11e-49	157
MsG0180001807.01.T01	MTR_4g058950	42.384	151	86	1	1	151	35	184	2.57e-38	129
MsG0180001807.01.T01	MTR_4g068780	37.086	151	95	0	1	151	15	165	1.46e-21	85.5
MsG0180001807.01.T01	MTR_8g078830	35.570	149	94	2	1	148	1	148	1.39e-19	80.1
MsG0180001807.01.T01	MTR_5g018680	35.526	152	96	2	1	151	1	151	7.45e-18	75.5
MsG0180001807.01.T01	MTR_4g113590	64.706	51	18	0	45	95	4	54	2.03e-17	72.0
MsG0180001807.01.T02	MTR_1g043450	92.105	152	1	1	1	141	1	152	3.77e-95	271

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180001807.01.T01	AT3G23200	62.914	151	56	0	1	151	1	151	5.66e-67	200
MsG0180001807.01.T01	AT3G53850	50.980	153	73	1	1	151	1	153	1.90e-50	159
MsG0180001807.01.T01	AT3G53850	50.980	153	73	1	1	151	1	153	1.90e-50	159
MsG0180001807.01.T01	AT3G23200	67.290	107	35	0	45	151	18	124	4.56e-48	152
MsG0180001807.01.T01	AT5G02060	49.669	151	74	1	1	149	1	151	1.98e-41	135
MsG0180001807.01.T01	AT3G53850	54.054	111	49	1	43	151	30	140	1.36e-36	123
MsG0180001807.01.T01	AT2G28370	43.709	151	84	1	1	151	29	178	1.59e-33	116
MsG0180001807.01.T01	AT2G37200	43.333	150	83	2	1	150	31	178	2.28e-32	113
MsG0180001807.01.T01	AT2G37200	43.333	150	83	2	1	150	60	207	5.46e-32	113
MsG0180001807.01.T01	AT4G37235	34.375	128	77	3	9	134	9	131	7.50e-19	78.2

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Find 17 sgRNAs with CRISPR-Local

Find 148 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AATTGAGATCCAGCTCATGA+AGG	0.614269	1:+27406467	None:intergenic
ACAAAGAGGCTAATCAATAC+AGG	0.449483	1:+27407045	None:intergenic
AGGCAAAAGCAACAGAAATC+TGG	0.443728	1:+27406487	None:intergenic
ATGCTTTGGTTAATAGCAGC+AGG	0.466883	1:-27406426	MsG0180001807.01.T04:CDS
CAAAGAGGCTAATCAATACA+GGG	0.608265	1:+27407046	None:intergenic
CAATGGCTTTACAGATCATT+TGG	0.334026	1:-27407128	MsG0180001807.01.T04:CDS
CAGTTTCTTGATAGCTTCAA+TGG	0.369991	1:-27407145	MsG0180001807.01.T04:CDS
CCCAATCTCCAACAACAAAG+AGG	0.575927	1:+27407031	None:intergenic
CCTCTTTGTTGTTGGAGATT+GGG	0.313786	1:-27407031	MsG0180001807.01.T04:intron
CTATTAACCAAAGCATTATT+AGG	0.170267	1:+27406433	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
ACTTAAGTGATGAATGCAAT+TGG	-	Chr1:27407473-27407492	MsG0180001807.01.T04:intron	30.0%
AGGTAAATGAGATATGAAGT+GGG	+	Chr1:27406568-27406587	None:intergenic	30.0%
ATATGCTTTGGTGAAAAAGA+AGG	-	Chr1:27408180-27408199	MsG0180001807.01.T04:intron	30.0%
ATCCAAACAAGACTTAAGTT+TGG	-	Chr1:27408612-27408631	MsG0180001807.01.T04:intron	30.0%
CTAACTTGAATCATGAATGA+TGG	+	Chr1:27406824-27406843	None:intergenic	30.0%
CTTAAGTGATGAATGCAATT+GGG	-	Chr1:27407474-27407493	MsG0180001807.01.T04:intron	30.0%
GACGTGTAATAAAAGAAACT+GGG	+	Chr1:27406527-27406546	None:intergenic	30.0%
GATGTGGTAAATCTTAATAC+TGG	-	Chr1:27408074-27408093	MsG0180001807.01.T04:intron	30.0%
GATTAATGATTCATTTGGAC+CGG	+	Chr1:27407607-27407626	None:intergenic	30.0%
GATTACACATCAAATGACAA+CGG	-	Chr1:27407170-27407189	MsG0180001807.01.T04:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr1	gene	27406422	27408859	27406422	ID=MsG0180001807.01;Name=MsG0180001807.01
Chr1	mRNA	27406422	27408859	27406422	ID=MsG0180001807.01.T01;Parent=MsG0180001807.01;Name=MsG0180001807.01.T01;_AED=0.50;_eAED=0.50;_QI=0\|0\|0\|1\|0\|0\|3\|0\|152
Chr1	exon	27407032	27407164	27407032	ID=MsG0180001807.01.T01:exon:34789;Parent=MsG0180001807.01.T01
Chr1	CDS	27408729	27408859	27408729	ID=MsG0180001807.01.T01:cds;Parent=MsG0180001807.01.T01
Chr1	CDS	27407032	27407164	27407032	ID=MsG0180001807.01.T01:cds;Parent=MsG0180001807.01.T01
Chr1	CDS	27406422	27406616	27406422	ID=MsG0180001807.01.T01:cds;Parent=MsG0180001807.01.T01
Chr1	mRNA	27406422	27408859	27406422	ID=MsG0180001807.01.T02;Parent=MsG0180001807.01;Name=MsG0180001807.01.T02;_AED=0.50;_eAED=0.50;_QI=0\|0\|0\|1\|0\|0\|3\|0\|141
Chr1	exon	27407065	27407164	27407065	ID=MsG0180001807.01.T02:exon:34791;Parent=MsG0180001807.01.T02
Chr1	CDS	27408729	27408859	27408729	ID=MsG0180001807.01.T02:cds;Parent=MsG0180001807.01.T02
Chr1	CDS	27407065	27407164	27407065	ID=MsG0180001807.01.T02:cds;Parent=MsG0180001807.01.T02
Chr1	CDS	27406422	27406616	27406422	ID=MsG0180001807.01.T02:cds;Parent=MsG0180001807.01.T02
Chr1	mRNA	27406422	27408859	27406422	ID=MsG0180001807.01.T03;Parent=MsG0180001807.01;Name=MsG0180001807.01.T03;_AED=0.50;_eAED=0.50;_QI=0\|0\|0\|1\|0\|0\|3\|0\|136
Chr1	exon	27407080	27407164	27407080	ID=MsG0180001807.01.T03:exon:34792;Parent=MsG0180001807.01.T03
Chr1	CDS	27408729	27408859	27408729	ID=MsG0180001807.01.T03:cds;Parent=MsG0180001807.01.T03
Chr1	CDS	27407080	27407164	27407080	ID=MsG0180001807.01.T03:cds;Parent=MsG0180001807.01.T03
Chr1	CDS	27406422	27406616	27406422	ID=MsG0180001807.01.T03:cds;Parent=MsG0180001807.01.T03
Chr1	mRNA	27406422	27408859	27406422	ID=MsG0180001807.01.T04;Parent=MsG0180001807.01;Name=MsG0180001807.01.T04;_AED=0.50;_eAED=0.50;_QI=0\|0\|0\|1\|0\|0\|3\|0\|132
Chr1	exon	27407092	27407164	27407092	ID=MsG0180001807.01.T04:exon:34793;Parent=MsG0180001807.01.T04
Chr1	CDS	27408729	27408859	27408729	ID=MsG0180001807.01.T04:cds;Parent=MsG0180001807.01.T04
Chr1	CDS	27407092	27407164	27407092	ID=MsG0180001807.01.T04:cds;Parent=MsG0180001807.01.T04
Chr1	CDS	27406422	27406616	27406422	ID=MsG0180001807.01.T04:cds;Parent=MsG0180001807.01.T04
Chr1	mRNA	27406422	27407148	27406422	ID=MsG0180001807.01.T05;Parent=MsG0180001807.01;Name=MsG0180001807.01.T05;_AED=0.50;_eAED=0.50;_QI=0\|0\|0\|1\|0\|0\|2\|0\|103
Chr1	exon	27407032	27407148	27407032	ID=MsG0180001807.01.T05:exon:34794;Parent=MsG0180001807.01.T05
Chr1	CDS	27407032	27407148	27407032	ID=MsG0180001807.01.T05:cds;Parent=MsG0180001807.01.T05
Chr1	CDS	27406422	27406616	27406422	ID=MsG0180001807.01.T05:cds;Parent=MsG0180001807.01.T05
Chr1	mRNA	27406422	27406547	27406422	ID=MsG0180001807.01.T06;Parent=MsG0180001807.01;Name=MsG0180001807.01.T06;_AED=0.50;_eAED=0.50;_QI=0\|-1\|0\|1\|-1\|0\|1\|0\|41
Chr1	exon	27406422	27406547	27406422	ID=MsG0180001807.01.T06:exon:34795;Parent=MsG0180001807.01.T06
Chr1	CDS	27406422	27406547	27406422	ID=MsG0180001807.01.T06:cds;Parent=MsG0180001807.01.T06

Gene Sequence

>MsG0180001807.01.T01

ATGAAGAATTTCTCTGGCACACCTGGTACTGTTCTTGGTTTGATTCTCAGAATGTCACAGTTCATTTTTGCTACTGGTTCAATTGCTTCTATTGCTACCACACCCAGTTTCTTTTATTACACGTCATTTTGTTTCTTGATAGCTTCAATGGCTTTACAGATCATTTGGAGTTTTGTGCTTGCTTTGTTGGATGCATATGCTTTGGTGAAAAAGAAGGTTCTGCTTAACCCTGTATTGATTAGCCTCTTTGTTGTTGGAGATTGGGTAACAGCAACAATATCTCTAGCTGCAGCTTCTGCCTCAGCTGGACTTACAGTTTTGTACTTCCATGATATGGGACATTGCCATTTTGGAGAGGAGTGTCAAAAGTACCAGATTTCTGTTGCTTTTGCCTTCATGAGCTGGATCTCAATTTCAATATCATCCCTAATAATGCTTTGGTTAATAGCAGCAGGTTAG

>MsG0180001807.01.T03

ATGAAGAATTTCTCTGGCACACCTGGTACTGTTCTTGGTTTGATTCTCAGAATGTCACAGTTCATTTTTGCTACTGGTTCAATTGCTTCTATTGCTACCACACCCAGTTTCTTTTATTACACGTCATTTTGTTTCTTGATAGCTTCAATGGCTTTACAGATCATTTGGAGTTTTGTGCTTGCTTTGTTGGATGCATATGCTTTGGTGAAAAAGAAGGTAACAGCAACAATATCTCTAGCTGCAGCTTCTGCCTCAGCTGGACTTACAGTTTTGTACTTCCATGATATGGGACATTGCCATTTTGGAGAGGAGTGTCAAAAGTACCAGATTTCTGTTGCTTTTGCCTTCATGAGCTGGATCTCAATTTCAATATCATCCCTAATAATGCTTTGGTTAATAGCAGCAGGTTAG

>MsG0180001807.01.T02

ATGAAGAATTTCTCTGGCACACCTGGTACTGTTCTTGGTTTGATTCTCAGAATGTCACAGTTCATTTTTGCTACTGGTTCAATTGCTTCTATTGCTACCACACCCAGTTTCTTTTATTACACGTCATTTTGTTTCTTGATAGCTTCAATGGCTTTACAGATCATTTGGAGTTTTGTGCTTGCTTTGTTGGATGCATATGCTTTGGTGAAAAAGAAGGTTCTGCTTAACCCTGTAACAGCAACAATATCTCTAGCTGCAGCTTCTGCCTCAGCTGGACTTACAGTTTTGTACTTCCATGATATGGGACATTGCCATTTTGGAGAGGAGTGTCAAAAGTACCAGATTTCTGTTGCTTTTGCCTTCATGAGCTGGATCTCAATTTCAATATCATCCCTAATAATGCTTTGGTTAATAGCAGCAGGTTAG

>MsG0180001807.01.T05

ATGGCTTTACAGATCATTTGGAGTTTTGTGCTTGCTTTGTTGGATGCATATGCTTTGGTGAAAAAGAAGGTTCTGCTTAACCCTGTATTGATTAGCCTCTTTGTTGTTGGAGATTGGGTAACAGCAACAATATCTCTAGCTGCAGCTTCTGCCTCAGCTGGACTTACAGTTTTGTACTTCCATGATATGGGACATTGCCATTTTGGAGAGGAGTGTCAAAAGTACCAGATTTCTGTTGCTTTTGCCTTCATGAGCTGGATCTCAATTTCAATATCATCCCTAATAATGCTTTGGTTAATAGCAGCAGGTTAG

>MsG0180001807.01.T06

ATGGGACATTGCCATTTTGGAGAGGAGTGTCAAAAGTACCAGATTTCTGTTGCTTTTGCCTTCATGAGCTGGATCTCAATTTCAATATCATCCCTAATAATGCTTTGGTTAATAGCAGCAGGTTAG

>MsG0180001807.01.T04

ATGAAGAATTTCTCTGGCACACCTGGTACTGTTCTTGGTTTGATTCTCAGAATGTCACAGTTCATTTTTGCTACTGGTTCAATTGCTTCTATTGCTACCACACCCAGTTTCTTTTATTACACGTCATTTTGTTTCTTGATAGCTTCAATGGCTTTACAGATCATTTGGAGTTTTGTGCTTGCTTTGTTGGATGCATATGCTTTGGTAACAGCAACAATATCTCTAGCTGCAGCTTCTGCCTCAGCTGGACTTACAGTTTTGTACTTCCATGATATGGGACATTGCCATTTTGGAGAGGAGTGTCAAAAGTACCAGATTTCTGTTGCTTTTGCCTTCATGAGCTGGATCTCAATTTCAATATCATCCCTAATAATGCTTTGGTTAATAGCAGCAGGTTAG

Protein sequence

>MsG0180001807.01.T01

MKNFSGTPGTVLGLILRMSQFIFATGSIASIATTPSFFYYTSFCFLIASMALQIIWSFVLALLDAYALVKKKVLLNPVLISLFVVGDWVTATISLAAASASAGLTVLYFHDMGHCHFGEECQKYQISVAFAFMSWISISISSLIMLWLIAAG*

>MsG0180001807.01.T03

MKNFSGTPGTVLGLILRMSQFIFATGSIASIATTPSFFYYTSFCFLIASMALQIIWSFVLALLDAYALVKKKVTATISLAAASASAGLTVLYFHDMGHCHFGEECQKYQISVAFAFMSWISISISSLIMLWLIAAG*

>MsG0180001807.01.T02

MKNFSGTPGTVLGLILRMSQFIFATGSIASIATTPSFFYYTSFCFLIASMALQIIWSFVLALLDAYALVKKKVLLNPVTATISLAAASASAGLTVLYFHDMGHCHFGEECQKYQISVAFAFMSWISISISSLIMLWLIAAG*

>MsG0180001807.01.T05

MALQIIWSFVLALLDAYALVKKKVLLNPVLISLFVVGDWVTATISLAAASASAGLTVLYFHDMGHCHFGEECQKYQISVAFAFMSWISISISSLIMLWLIAAG*

>MsG0180001807.01.T06

MGHCHFGEECQKYQISVAFAFMSWISISISSLIMLWLIAAG*

>MsG0180001807.01.T04

MKNFSGTPGTVLGLILRMSQFIFATGSIASIATTPSFFYYTSFCFLIASMALQIIWSFVLALLDAYALVTATISLAAASASAGLTVLYFHDMGHCHFGEECQKYQISVAFAFMSWISISISSLIMLWLIAAG*