AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0180002083.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180002083.01.T01 MTR_1g036490 88.889 144 15 1 1 143 1 144 1.56e-83 253
MsG0180002083.01.T01 MTR_1g036510 92.308 143 11 0 1 143 16 158 8.49e-82 245
MsG0180002083.01.T01 MTR_1g036510 92.308 143 11 0 1 143 16 158 2.37e-81 245
MsG0180002083.01.T01 MTR_1g036510 92.308 143 11 0 1 143 16 158 5.09e-80 244
MsG0180002083.01.T01 MTR_1g036460 84.722 144 21 1 1 143 1 144 1.51e-79 243
MsG0180002083.01.T01 MTR_1g050220 88.800 125 14 0 21 145 5 129 5.86e-73 224
MsG0180002083.01.T01 MTR_7g011900 48.980 147 64 5 3 143 5 146 1.63e-32 121
MsG0180002083.01.T01 MTR_7g012040 49.624 133 61 3 12 143 17 144 2.91e-31 117
MsG0180002083.01.T01 MTR_7g012020 50.000 134 60 4 12 143 17 145 5.36e-30 112
MsG0180002083.01.T01 MTR_7g012020 48.507 134 62 4 12 143 17 145 2.69e-29 111
MsG0180002083.01.T01 MTR_7g012020 50.000 134 60 4 12 143 17 145 5.23e-29 112
MsG0180002083.01.T01 MTR_3g021440 51.181 127 56 3 11 135 16 138 5.53e-29 112
MsG0180002083.01.T01 MTR_2g055940 50.806 124 55 3 22 143 20 139 9.08e-29 111
MsG0180002083.01.T01 MTR_1g096310 45.205 146 67 4 1 143 13 148 1.27e-27 108
MsG0180002083.01.T01 MTR_7g011990 45.185 135 62 4 12 143 18 143 1.93e-25 102
MsG0180002083.01.T01 MTR_7g012070 43.056 144 72 5 12 151 18 155 6.19e-23 95.5
MsG0180002083.01.T01 MTR_3g054290 70.769 65 19 0 159 223 23 87 6.37e-23 89.0
MsG0180002083.01.T01 MTR_3g021430 45.455 132 65 4 15 143 7 134 1.12e-22 94.7
MsG0180002083.01.T01 MTR_4g088140 71.212 66 19 0 158 223 11 76 2.09e-22 87.4
MsG0180002083.01.T01 MTR_7g012090 43.262 141 71 5 16 151 19 155 2.67e-22 94.0
MsG0180002083.01.T01 MTR_3g092900 36.364 143 82 2 4 140 3 142 5.74e-22 92.8
MsG0180002083.01.T01 MTR_0029s0130 68.182 66 16 1 158 223 6 66 1.57e-21 85.5
MsG0180002083.01.T01 MTR_0179s0040 66.154 65 22 0 159 223 143 207 4.70e-18 80.1
MsG0180002083.01.T01 MTR_1g053785 64.615 65 19 1 158 222 21 81 1.89e-17 75.5
MsG0180002083.01.T01 MTR_6g060290 38.732 142 62 5 12 151 18 136 1.11e-16 75.1
MsG0180002083.01.T01 MTR_8g068740 36.975 119 70 2 18 134 29 144 2.09e-16 76.3
MsG0180002083.01.T01 MTR_8g068730 36.975 119 70 2 18 134 29 144 4.58e-16 76.3
MsG0180002083.01.T01 MTR_5g014780 69.231 52 16 0 158 209 49 100 5.78e-16 71.2
MsG0180002083.01.T01 MTR_8g068750 34.000 150 89 4 18 165 29 170 2.26e-15 74.3
MsG0180002083.01.T01 MTR_8g068760 34.351 131 78 4 20 146 18 144 4.08e-15 72.0
MsG0180002083.01.T01 MTR_8g068590 31.746 126 81 2 13 136 3 125 2.35e-14 68.2
MsG0180002083.01.T01 MTR_7g118300 37.037 135 79 4 13 145 24 154 6.60e-14 70.1
MsG0180002083.01.T01 MTR_8g068690 31.788 151 91 5 18 165 28 169 1.04e-13 68.9
MsG0180002083.01.T01 MTR_7g118320 34.375 128 77 3 11 134 5 129 1.13e-13 69.3
MsG0180002083.01.T01 MTR_5g056120 63.636 55 20 0 158 212 6 60 1.24e-13 65.5
MsG0180002083.01.T01 MTR_0591s0010 30.464 151 93 4 18 165 29 170 1.42e-13 69.3
MsG0180002083.01.T01 MTR_8g068690 31.788 151 91 5 18 165 28 169 2.21e-13 68.6
MsG0180002083.01.T01 MTR_4g127850 73.913 46 12 0 158 203 6 51 2.72e-13 63.2
MsG0180002083.01.T01 MTR_8g068870 29.412 153 96 4 13 162 10 153 1.02e-12 66.6
MsG0180002083.01.T01 MTR_5g098170 35.294 119 71 3 13 128 9 124 1.11e-12 66.6
MsG0180002083.01.T01 MTR_8g068570 31.933 119 76 2 18 134 29 144 1.03e-11 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180002083.01.T01 AT5G54160 34.965 143 82 2 4 140 3 140 1.07e-16 78.2
MsG0180002083.01.T01 AT1G33030 35.032 157 87 6 24 177 10 154 3.21e-14 70.1
MsG0180002083.01.T01 AT1G33030 35.032 157 87 6 24 177 10 154 4.33e-14 70.1
MsG0180002083.01.T01 AT1G33030 35.032 157 87 6 24 177 10 154 1.24e-13 69.3
MsG0180002083.01.T01 AT1G77520 40.157 127 61 7 33 151 42 161 2.43e-12 65.9

Find 40 sgRNAs with CRISPR-Local

Find 184 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGATAAAGATGGAGGCTCTT+TGG 0.282636 1:+32409549 MsG0180002083.01.T01:CDS
GCCAATTCAAGGTGTTGATT+TGG 0.318990 1:-32409397 None:intergenic
GATAAAGATGGAGGCTCTTT+GGG 0.323496 1:+32409550 MsG0180002083.01.T01:CDS
CGATTAAATTTCAAATTACT+AGG 0.327343 1:-32409834 None:intergenic
TTCTCTCTTACCATCTTCAT+TGG 0.344842 1:-32409485 None:intergenic
CATGCCAGGGCTTTCGCTCC+TGG 0.367494 1:+32411852 MsG0180002083.01.T01:CDS
TCCAAATCAACACCTTGAAT+TGG 0.367755 1:+32409396 MsG0180002083.01.T01:CDS
ATAGCAGCATTTAGTATTGC+AGG 0.379466 1:-32409286 None:intergenic
AAAGACCCTGGCACAACTCC+TGG 0.437760 1:+32411891 MsG0180002083.01.T01:CDS
ATCGTGGATATCATGATGTT+TGG 0.437952 1:+32409593 MsG0180002083.01.T01:CDS
TTTGCTAGTGATTCTGCTCC+TGG 0.441290 1:-32411810 None:intergenic
GTTTATGCTCTCTCATCAAT+TGG 0.442550 1:+32409511 MsG0180002083.01.T01:CDS
GTTGTGCCAGGGTCTTTGCC+AGG 0.453176 1:-32411885 None:intergenic
TACTTTGCATTGGATAAAGA+TGG 0.457504 1:+32409538 MsG0180002083.01.T01:CDS
CAAGCCTCTCAGCCAATTCA+AGG 0.458166 1:-32409408 None:intergenic
TTGCCAGGAGCGAATTTGCC+AGG 0.459619 1:-32411870 None:intergenic
CGTTTGCCAGGAGCTTTGCC+AGG 0.460797 1:+32411792 MsG0180002083.01.T01:CDS
TCTGCTCCTGGCAAAGCTCC+TGG 0.460974 1:-32411798 None:intergenic
TTGGCAAATGTACGTTTGCC+AGG 0.466939 1:+32411780 MsG0180002083.01.T01:CDS
AATTGGTCAATACTTTGCAT+TGG 0.471367 1:+32409528 MsG0180002083.01.T01:CDS
TCCATTCGTACCAATGAAGA+TGG 0.486141 1:+32409475 MsG0180002083.01.T01:CDS
CCATCGCAAATGAACATGCC+AGG 0.491125 1:+32411838 MsG0180002083.01.T01:CDS
GCTCCTGGCAAATTCGCTCC+TGG 0.494329 1:+32411867 MsG0180002083.01.T01:CDS
ACCATCTTCATTGGTACGAA+TGG 0.499723 1:-32409476 None:intergenic
TGAACGCATGTTACCTGTGT+TGG 0.505564 1:+32409429 MsG0180002083.01.T01:CDS
AAACTGAGTTTGTTTGGTGT+TGG 0.509463 1:-32409194 None:intergenic
CTGGCATGTTCATTTGCGAT+GGG 0.536882 1:-32411837 None:intergenic
CCTGGCATGTTCATTTGCGA+TGG 0.540000 1:-32411838 None:intergenic
TTCGCTCCTGGCAAAGACCC+TGG 0.542210 1:+32411879 MsG0180002083.01.T01:CDS
TGTCAAAATGCAGAAAGCCT+TGG 0.563490 1:+32411761 MsG0180002083.01.T01:CDS
TTTGCCAGGAGCGAAAGCCC+TGG 0.584171 1:-32411856 None:intergenic
TAATTTGAAATTTAATCGAG+AGG 0.592207 1:+32409839 MsG0180002083.01.T01:CDS
AATTTAATCGAGAGGTACCT+AGG 0.592378 1:+32409847 MsG0180002083.01.T01:CDS
TGGCAAACGTACATTTGCCA+AGG 0.601692 1:-32411778 None:intergenic
CATCGCAAATGAACATGCCA+GGG 0.603987 1:+32411839 MsG0180002083.01.T01:CDS
AGAGAGTAACTAGCCAACAC+AGG 0.615481 1:-32409442 None:intergenic
AACACCTTGAATTGGCTGAG+AGG 0.622154 1:+32409404 MsG0180002083.01.T01:CDS
CTCTCAGCATTAATCCATCG+TGG 0.628716 1:+32409577 MsG0180002083.01.T01:CDS
TTTGCATTGGATAAAGATGG+AGG 0.638338 1:+32409541 MsG0180002083.01.T01:CDS
AAACATCATGATATCCACGA+TGG 0.677658 1:-32409591 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATATATATATATATATTACC+AGG - Chr1:32410223-32410242 None:intergenic 10.0%
!! GAATTTAATTAAGAAAATAA+AGG + Chr1:32411244-32411263 MsG0180002083.01.T01:intron 10.0%
!!! AAAATATTTTTCTATACTAA+CGG + Chr1:32411342-32411361 MsG0180002083.01.T01:intron 10.0%
!!! TGACTATTTTTATTTTATTA+TGG + Chr1:32409779-32409798 MsG0180002083.01.T01:intron 10.0%
!!! TTTTATTTGAAAAAAAGTTA+TGG + Chr1:32410930-32410949 MsG0180002083.01.T01:intron 10.0%
!! AGTTTAATTAAGATTCATTT+AGG + Chr1:32411222-32411241 MsG0180002083.01.T01:intron 15.0%
!! ATATTATGAAAATAGAGAAA+TGG - Chr1:32410637-32410656 None:intergenic 15.0%
!! TATGAATGAATTGAATAATT+AGG + Chr1:32410372-32410391 MsG0180002083.01.T01:intron 15.0%
!!! ATTTCTCTATTTTCATAATA+TGG + Chr1:32410636-32410655 MsG0180002083.01.T01:intron 15.0%
!!! GTGTTTGTTTTTTTTTTTTT+TGG - Chr1:32411704-32411723 None:intergenic 15.0%
!!! TTGTTTTTATTTGATTGATT+TGG + Chr1:32411002-32411021 MsG0180002083.01.T01:intron 15.0%
!!! TTTCTATTTTTTTGTTTTGT+TGG + Chr1:32409670-32409689 MsG0180002083.01.T01:intron 15.0%
!!! TTTTCCAAATATTTATAAGT+CGG - Chr1:32409976-32409995 None:intergenic 15.0%
!! AAAAAATCTAAGTGATAGAA+TGG + Chr1:32410957-32410976 MsG0180002083.01.T01:intron 20.0%
!! AAAGAATGGAATATCATTAA+TGG + Chr1:32410403-32410422 MsG0180002083.01.T01:intron 20.0%
!! CGATTAAATTTCAAATTACT+AGG - Chr1:32409837-32409856 None:intergenic 20.0%
!!! AAATTAATGCCTTTTATTGA+AGG - Chr1:32410163-32410182 None:intergenic 20.0%
!!! AGTATTTTCTTAATAAACCT+TGG - Chr1:32411290-32411309 None:intergenic 20.0%
!!! ATGATATTAGTGTGTATTTT+AGG - Chr1:32411537-32411556 None:intergenic 20.0%
!!! ATTATGTTGTTTCTTTTTTC+AGG + Chr1:32409884-32409903 MsG0180002083.01.T01:intron 20.0%
!!! TAATTTGAAATTTAATCGAG+AGG + Chr1:32409839-32409858 MsG0180002083.01.T01:CDS 20.0%
!!! TATATTCTTCTCTTTTATAG+TGG + Chr1:32410330-32410349 MsG0180002083.01.T01:intron 20.0%
!!! TCAAATTTTTAACAAAGCAA+TGG + Chr1:32410012-32410031 MsG0180002083.01.T01:intron 20.0%
!!! TGATTCATGATCTAATATTT+TGG - Chr1:32411632-32411651 None:intergenic 20.0%
!!! TTCTCTCTTAAAAGTATTTT+TGG + Chr1:32411078-32411097 MsG0180002083.01.T01:intron 20.0%
!!! TTTATTCAAACTTTTCTTCA+AGG + Chr1:32409629-32409648 MsG0180002083.01.T01:intron 20.0%
!!! TTTGAAAATGGTTTTAAGAA+GGG + Chr1:32411125-32411144 MsG0180002083.01.T01:intron 20.0%
!!! TTTTATTTGATTGATTTGGA+AGG + Chr1:32411006-32411025 MsG0180002083.01.T01:intron 20.0%
!!! TTTTGAAAATGGTTTTAAGA+AGG + Chr1:32411124-32411143 MsG0180002083.01.T01:intron 20.0%
!!! TTTTTTTTGGTACAAAAAGT+TGG - Chr1:32411691-32411710 None:intergenic 20.0%
! AAAATAAAGGGAAAACACTT+GGG + Chr1:32411257-32411276 MsG0180002083.01.T01:intron 25.0%
! AAAGTACAACCAGTATTTAT+AGG - Chr1:32410582-32410601 None:intergenic 25.0%
! AATGATATTCCATTCTTTCT+AGG - Chr1:32410401-32410420 None:intergenic 25.0%
! ACACTATATAAAGGATTTGA+GGG + Chr1:32410070-32410089 MsG0180002083.01.T01:intron 25.0%
! AGGATTCTTACACTATATAA+AGG + Chr1:32410061-32410080 MsG0180002083.01.T01:intron 25.0%
! AGTTTGATTAGCATGTAATT+AGG + Chr1:32410537-32410556 MsG0180002083.01.T01:intron 25.0%
! ATCCATGAACTTTATGAAAA+CGG - Chr1:32409951-32409970 None:intergenic 25.0%
! CTAGAAATAAAATAGAAGTG+AGG + Chr1:32411168-32411187 MsG0180002083.01.T01:intron 25.0%
! TAAATATAGTGATGTTGTGT+CGG + Chr1:32411410-32411429 MsG0180002083.01.T01:intron 25.0%
! TACACTATATAAAGGATTTG+AGG + Chr1:32410069-32410088 MsG0180002083.01.T01:intron 25.0%
! TCTGAATATCCTTCAATAAA+AGG + Chr1:32410151-32410170 MsG0180002083.01.T01:intron 25.0%
! TTTGCAATTCCTATAAATAC+TGG + Chr1:32410570-32410589 MsG0180002083.01.T01:intron 25.0%
!! AGAATCCTTTTCATTTCTAA+TGG - Chr1:32410049-32410068 None:intergenic 25.0%
!! TATTATGGTGTTAACATTCT+GGG + Chr1:32409794-32409813 MsG0180002083.01.T01:intron 25.0%
!! TTATTATGGTGTTAACATTC+TGG + Chr1:32409793-32409812 MsG0180002083.01.T01:intron 25.0%
!!! ATACTTTTAAGAGAGAACTA+CGG - Chr1:32411075-32411094 None:intergenic 25.0%
!!! ATCACCGACTTATAAATATT+TGG + Chr1:32409969-32409988 MsG0180002083.01.T01:intron 25.0%
!!! CATTGTTACTATGTTTTAGT+AGG + Chr1:32409257-32409276 MsG0180002083.01.T01:CDS 25.0%
!!! CTCTTAAAAGTATTTTTGGT+GGG + Chr1:32411082-32411101 MsG0180002083.01.T01:intron 25.0%
!!! GATGAATTTAAAACCCTTTT+TGG + Chr1:32410808-32410827 MsG0180002083.01.T01:intron 25.0%
!!! TCTCTTAAAAGTATTTTTGG+TGG + Chr1:32411081-32411100 MsG0180002083.01.T01:intron 25.0%
!!! TTGAAAATGGTTTTAAGAAG+GGG + Chr1:32411126-32411145 MsG0180002083.01.T01:intron 25.0%
!!! TTTTATTTCTTGCCTTAGAA+TGG - Chr1:32411487-32411506 None:intergenic 25.0%
AAATCTAAGTGATAGAATGG+AGG + Chr1:32410960-32410979 MsG0180002083.01.T01:intron 30.0%
AATCGTATTGAACAATACCT+AGG - Chr1:32409867-32409886 None:intergenic 30.0%
AATCTAAGTGATAGAATGGA+GGG + Chr1:32410961-32410980 MsG0180002083.01.T01:intron 30.0%
AATTGGTCAATACTTTGCAT+TGG + Chr1:32409528-32409547 MsG0180002083.01.T01:CDS 30.0%
ATTAGGAATCCTAGAAAGAA+TGG + Chr1:32410389-32410408 MsG0180002083.01.T01:intron 30.0%
CACAAGATAAAGTTAGAAAG+AGG - Chr1:32410901-32410920 None:intergenic 30.0%
GAAAATAAAGGGAAAACACT+TGG + Chr1:32411256-32411275 MsG0180002083.01.T01:intron 30.0%
GAATGGAATATCATTAATGG+AGG + Chr1:32410406-32410425 MsG0180002083.01.T01:intron 30.0%
TACTTTGCATTGGATAAAGA+TGG + Chr1:32409538-32409557 MsG0180002083.01.T01:CDS 30.0%
TATATATTACCAGGATGAGT+TGG - Chr1:32410214-32410233 None:intergenic 30.0%
! ATCTTGTAGACCATGATTTT+CGG + Chr1:32410712-32410731 MsG0180002083.01.T01:intron 30.0%
! TGTTAACATTCTGGGTTTTA+AGG + Chr1:32409802-32409821 MsG0180002083.01.T01:intron 30.0%
!! GTTAACATTCTGGGTTTTAA+GGG + Chr1:32409803-32409822 MsG0180002083.01.T01:intron 30.0%
!! TTTAAAACCCTTTTTGGATC+CGG + Chr1:32410814-32410833 MsG0180002083.01.T01:intron 30.0%
!!! AAAAGTATTTTTGGTGGGTT+AGG + Chr1:32411087-32411106 MsG0180002083.01.T01:intron 30.0%
!!! ATTTTATTTCTAGAGCCTTG+AGG - Chr1:32411161-32411180 None:intergenic 30.0%
!!! TAATTTTGATTTACCCCAAG+TGG + Chr1:32410177-32410196 MsG0180002083.01.T01:intron 30.0%
AAACATCATGATATCCACGA+TGG - Chr1:32409594-32409613 None:intergenic 35.0%
AAGTTAGAAAGAGGTTCTCA+CGG - Chr1:32410892-32410911 None:intergenic 35.0%
ATAGCAGCATTTAGTATTGC+AGG - Chr1:32409289-32409308 None:intergenic 35.0%
ATCGTGGATATCATGATGTT+TGG + Chr1:32409593-32409612 MsG0180002083.01.T01:CDS 35.0%
CTGGTCCATTAGAAATGAAA+AGG + Chr1:32410041-32410060 MsG0180002083.01.T01:intron 35.0%
GAAGGTTCAAAACCAATCAA+TGG - Chr1:32410746-32410765 None:intergenic 35.0%
GTTTATGCTCTCTCATCAAT+TGG + Chr1:32409511-32409530 MsG0180002083.01.T01:CDS 35.0%
TAATGGAGGATCAGTATTAG+AGG + Chr1:32410420-32410439 MsG0180002083.01.T01:intron 35.0%
TCCAAATCAACACCTTGAAT+TGG + Chr1:32409396-32409415 MsG0180002083.01.T01:CDS 35.0%
TTCTCTCTTACCATCTTCAT+TGG - Chr1:32409488-32409507 None:intergenic 35.0%
TTTGCATTGGATAAAGATGG+AGG + Chr1:32409541-32409560 MsG0180002083.01.T01:CDS 35.0%
! AAGAAATGTTGCATTTTGCC+AGG + Chr1:32411727-32411746 MsG0180002083.01.T01:intron 35.0%
! AGAAATGTTGCATTTTGCCA+GGG + Chr1:32411728-32411747 MsG0180002083.01.T01:intron 35.0%
! GAAAACGGTCATTGTTGTTA+GGG - Chr1:32409936-32409955 None:intergenic 35.0%
! GACCGTTTTCATAAAGTTCA+TGG + Chr1:32409946-32409965 MsG0180002083.01.T01:intron 35.0%
! TCTTGTAGACCATGATTTTC+GGG + Chr1:32410713-32410732 MsG0180002083.01.T01:intron 35.0%
! TGAAAACGGTCATTGTTGTT+AGG - Chr1:32409937-32409956 None:intergenic 35.0%
! TTAAAACCCTTTTTGGATCC+GGG + Chr1:32410815-32410834 MsG0180002083.01.T01:intron 35.0%
!! AAACTGAGTTTGTTTGGTGT+TGG - Chr1:32409197-32409216 None:intergenic 35.0%
!! AATTTAATCGAGAGGTACCT+AGG + Chr1:32409847-32409866 MsG0180002083.01.T01:CDS 35.0%
!! GTTTTGAACCTTCAATCGTT+TGG + Chr1:32410753-32410772 MsG0180002083.01.T01:intron 35.0%
!!! ATCAACTTTTTCAACCTCAG+GGG - Chr1:32409221-32409240 None:intergenic 35.0%
!!! CATCAACTTTTTCAACCTCA+GGG - Chr1:32409222-32409241 None:intergenic 35.0%
!!! TCATCAACTTTTTCAACCTC+AGG - Chr1:32409223-32409242 None:intergenic 35.0%
AACAAAGCAATGGCTCAATC+TGG + Chr1:32410022-32410041 MsG0180002083.01.T01:intron 40.0%
AAGGGAAAACACTTGGGTTA+CGG + Chr1:32411263-32411282 MsG0180002083.01.T01:intron 40.0%
ACCATCTTCATTGGTACGAA+TGG - Chr1:32409479-32409498 None:intergenic 40.0%
ACTAAGCATGCTAAGAGACT+TGG - Chr1:32410509-32410528 None:intergenic 40.0%
ATGGAGGAGAAAAAAAGCTC+TGG - Chr1:32410665-32410684 None:intergenic 40.0%
ATTTGAGGGAGTCTCAACAT+TGG + Chr1:32410084-32410103 MsG0180002083.01.T01:intron 40.0%
CCTTAGAATGGATCTAGGAA+GGG - Chr1:32411475-32411494 None:intergenic 40.0%
CGAAAAGTCCAAACGATTGA+AGG - Chr1:32410764-32410783 None:intergenic 40.0%
CTGCCTATGTACCCTTAAAA+GGG + Chr1:32411039-32411058 MsG0180002083.01.T01:intron 40.0%
GATAAAGATGGAGGCTCTTT+GGG + Chr1:32409550-32409569 MsG0180002083.01.T01:CDS 40.0%
TCCATTCGTACCAATGAAGA+TGG + Chr1:32409475-32409494 MsG0180002083.01.T01:CDS 40.0%
TCGATCTTTATTCGTGCGAA+GGG + Chr1:32410781-32410800 MsG0180002083.01.T01:intron 40.0%
TCTGCCTATGTACCCTTAAA+AGG + Chr1:32411038-32411057 MsG0180002083.01.T01:intron 40.0%
TCTTGCCTTAGAATGGATCT+AGG - Chr1:32411480-32411499 None:intergenic 40.0%
TGTCAAAATGCAGAAAGCCT+TGG + Chr1:32411761-32411780 MsG0180002083.01.T01:CDS 40.0%
TTCGATCTTTATTCGTGCGA+AGG + Chr1:32410780-32410799 MsG0180002083.01.T01:intron 40.0%
! GAAATGTTGCATTTTGCCAG+GGG + Chr1:32411729-32411748 MsG0180002083.01.T01:intron 40.0%
! GTCTCAACATTGGTTGATGT+AGG + Chr1:32410094-32410113 MsG0180002083.01.T01:intron 40.0%
! TAAAACCCTTTTTGGATCCG+GGG + Chr1:32410816-32410835 MsG0180002083.01.T01:intron 40.0%
! TCAACATTGGTTGATGTAGG+TGG + Chr1:32410097-32410116 MsG0180002083.01.T01:intron 40.0%
! TTTAAGGGTACATAGGCAGA+GGG - Chr1:32411038-32411057 None:intergenic 40.0%
!! AAGGCTTTCTGCATTTTGAC+AGG - Chr1:32411762-32411781 None:intergenic 40.0%
!! AGGCTTTCTGCATTTTGACA+GGG - Chr1:32411761-32411780 None:intergenic 40.0%
!! CTTTTAAGAGAGAACTACGG+CGG - Chr1:32411072-32411091 None:intergenic 40.0%
!! GATCCCTTTTAAGGGTACAT+AGG - Chr1:32411045-32411064 None:intergenic 40.0%
!! GCCAATTCAAGGTGTTGATT+TGG - Chr1:32409400-32409419 None:intergenic 40.0%
!! GCGGTTTTGATCCCTTTTAA+GGG - Chr1:32411053-32411072 None:intergenic 40.0%
!! TTTTAAGGGTACATAGGCAG+AGG - Chr1:32411039-32411058 None:intergenic 40.0%
!!! CTTTTTTTCTCCTCCATCCA+TGG + Chr1:32410668-32410687 MsG0180002083.01.T01:intron 40.0%
!!! GGTTTTAAGAAGGGGATTGA+GGG + Chr1:32411134-32411153 MsG0180002083.01.T01:intron 40.0%
!!! GTTTTAAGAAGGGGATTGAG+GGG + Chr1:32411135-32411154 MsG0180002083.01.T01:intron 40.0%
!!! TCAACTTTTTCAACCTCAGG+GGG - Chr1:32409220-32409239 None:intergenic 40.0%
!!! TGGTTTTAAGAAGGGGATTG+AGG + Chr1:32411133-32411152 MsG0180002083.01.T01:intron 40.0%
AACACCTTGAATTGGCTGAG+AGG + Chr1:32409404-32409423 MsG0180002083.01.T01:CDS 45.0%
AACTAACTTTCCGCCATGGA+TGG - Chr1:32410684-32410703 None:intergenic 45.0%
AGAGAGTAACTAGCCAACAC+AGG - Chr1:32409445-32409464 None:intergenic 45.0%
CATCGCAAATGAACATGCCA+GGG + Chr1:32411839-32411858 MsG0180002083.01.T01:CDS 45.0%
CCCTTCCTAGATCCATTCTA+AGG + Chr1:32411472-32411491 MsG0180002083.01.T01:intron 45.0%
CTCTCAGCATTAATCCATCG+TGG + Chr1:32409577-32409596 MsG0180002083.01.T01:CDS 45.0%
CTGGCATGTTCATTTGCGAT+GGG - Chr1:32411840-32411859 None:intergenic 45.0%
CTGTAACTAACTTTCCGCCA+TGG - Chr1:32410688-32410707 None:intergenic 45.0%
GCCTTAGAATGGATCTAGGA+AGG - Chr1:32411476-32411495 None:intergenic 45.0%
GGATAAAGATGGAGGCTCTT+TGG + Chr1:32409549-32409568 MsG0180002083.01.T01:CDS 45.0%
GGATTGACACCCGAAAATCA+TGG - Chr1:32410725-32410744 None:intergenic 45.0%
TCGGGTGTCAATCCATTGAT+TGG + Chr1:32410731-32410750 MsG0180002083.01.T01:intron 45.0%
TGAACGCATGTTACCTGTGT+TGG + Chr1:32409429-32409448 MsG0180002083.01.T01:CDS 45.0%
TGGCAAACGTACATTTGCCA+AGG - Chr1:32411781-32411800 None:intergenic 45.0%
TGGTGCATCTTGAACCACTT+GGG - Chr1:32410194-32410213 None:intergenic 45.0%
TTGGCAAATGTACGTTTGCC+AGG + Chr1:32411780-32411799 MsG0180002083.01.T01:CDS 45.0%
TTGGTGCATCTTGAACCACT+TGG - Chr1:32410195-32410214 None:intergenic 45.0%
TTTGCTAGTGATTCTGCTCC+TGG - Chr1:32411813-32411832 None:intergenic 45.0%
! AAAACCCTTTTTGGATCCGG+GGG + Chr1:32410817-32410836 MsG0180002083.01.T01:intron 45.0%
! ACATTGGTTGATGTAGGTGG+TGG + Chr1:32410100-32410119 MsG0180002083.01.T01:intron 45.0%
! AGGGGGAAACTGAGTTTGTT+TGG - Chr1:32409203-32409222 None:intergenic 45.0%
! TCTATCAGACGCATTTTGCC+AGG - Chr1:32411912-32411931 None:intergenic 45.0%
! TTTTTCTCCTCCATCCATGG+CGG + Chr1:32410671-32410690 MsG0180002083.01.T01:intron 45.0%
!! CGGAGTACTGTGATCTTTAG+AGG - Chr1:32410872-32410891 None:intergenic 45.0%
!! GGCGGTTTTGATCCCTTTTA+AGG - Chr1:32411054-32411073 None:intergenic 45.0%
!! TTGATGTAGGTGGTGGAGTA+GGG + Chr1:32410107-32410126 MsG0180002083.01.T01:intron 45.0%
!! AATTTAATTAAGAAAATAAA+GGG + Chr1:32411245-32411264 MsG0180002083.01.T01:intron 5.0%
!!! ATTTTAATTATTTTTGAAAA+TGG + Chr1:32411113-32411132 MsG0180002083.01.T01:intron 5.0%
AACACTTGGGTTACGGACCA+AGG + Chr1:32411270-32411289 MsG0180002083.01.T01:intron 50.0%
ACAAACTCAGTTTCCCCCTG+AGG + Chr1:32409204-32409223 MsG0180002083.01.T01:CDS 50.0%
CAAGATGCACCAACTCATCC+TGG + Chr1:32410202-32410221 MsG0180002083.01.T01:intron 50.0%
CAAGCCTCTCAGCCAATTCA+AGG - Chr1:32409411-32409430 None:intergenic 50.0%
CCATCGCAAATGAACATGCC+AGG + Chr1:32411838-32411857 MsG0180002083.01.T01:CDS 50.0%
CCTGGCATGTTCATTTGCGA+TGG - Chr1:32411841-32411860 None:intergenic 50.0%
GGTGCATCTTGAACCACTTG+GGG - Chr1:32410193-32410212 None:intergenic 50.0%
TAACTTTCCGCCATGGATGG+AGG - Chr1:32410681-32410700 None:intergenic 50.0%
! ATTTTGCCAGGAGTTGTGCC+AGG - Chr1:32411900-32411919 None:intergenic 50.0%
! TGTTGTGTCGGTGCTTGTGT+CGG + Chr1:32411422-32411441 MsG0180002083.01.T01:intron 50.0%
! TTTTGCCAGGAGTTGTGCCA+GGG - Chr1:32411899-32411918 None:intergenic 50.0%
!! GGGTTGGTTTCGATAGAGGA+AGG + Chr1:32410837-32410856 MsG0180002083.01.T01:intron 50.0%
!! GTTGATGTAGGTGGTGGAGT+AGG + Chr1:32410106-32410125 MsG0180002083.01.T01:intron 50.0%
AAAGACCCTGGCACAACTCC+TGG + Chr1:32411891-32411910 MsG0180002083.01.T01:CDS 55.0%
ACCAACCCCCGGATCCAAAA+AGG - Chr1:32410825-32410844 None:intergenic 55.0%
CCAACCCCCGGATCCAAAAA+GGG - Chr1:32410824-32410843 None:intergenic 55.0%
CCTCTATCGAAACCAACCCC+CGG - Chr1:32410836-32410855 None:intergenic 55.0%
TTGCCAGGAGCGAATTTGCC+AGG - Chr1:32411873-32411892 None:intergenic 55.0%
AAGGGGATTGAGGGGCCTCA+AGG + Chr1:32411143-32411162 MsG0180002083.01.T01:intron 60.0%
CGTTTGCCAGGAGCTTTGCC+AGG + Chr1:32411792-32411811 MsG0180002083.01.T01:CDS 60.0%
GCTCCTGGCAAATTCGCTCC+TGG + Chr1:32411867-32411886 MsG0180002083.01.T01:CDS 60.0%
GTTGTGCCAGGGTCTTTGCC+AGG - Chr1:32411888-32411907 None:intergenic 60.0%
TCTGCTCCTGGCAAAGCTCC+TGG - Chr1:32411801-32411820 None:intergenic 60.0%
TTCGCTCCTGGCAAAGACCC+TGG + Chr1:32411879-32411898 MsG0180002083.01.T01:CDS 60.0%
TTTGACAGGGCTTCTGCCCC+TGG - Chr1:32411748-32411767 None:intergenic 60.0%
TTTGCCAGGAGCGAAAGCCC+TGG - Chr1:32411859-32411878 None:intergenic 60.0%
! CCCTTTTTGGATCCGGGGGT+TGG + Chr1:32410821-32410840 MsG0180002083.01.T01:intron 60.0%
!! CCGGGGGTTGGTTTCGATAG+AGG + Chr1:32410833-32410852 MsG0180002083.01.T01:intron 60.0%
CATGCCAGGGCTTTCGCTCC+TGG + Chr1:32411852-32411871 MsG0180002083.01.T01:CDS 65.0%
Chromosome Type Strat End Strand Name
Chr1 gene 32409154 32411947 32409154 ID=MsG0180002083.01;Name=MsG0180002083.01
Chr1 mRNA 32409154 32411947 32409154 ID=MsG0180002083.01.T01;Parent=MsG0180002083.01;Name=MsG0180002083.01.T01;_AED=0.43;_eAED=0.48;_QI=33|0|0.33|0.66|1|1|3|0|223
Chr1 exon 32411749 32411947 32411749 ID=MsG0180002083.01.T01:exon:7562;Parent=MsG0180002083.01.T01
Chr1 exon 32409824 32409868 32409824 ID=MsG0180002083.01.T01:exon:7561;Parent=MsG0180002083.01.T01
Chr1 exon 32409154 32409614 32409154 ID=MsG0180002083.01.T01:exon:7560;Parent=MsG0180002083.01.T01
Chr1 five_prime_UTR 32409154 32409186 32409154 ID=MsG0180002083.01.T01:five_prime_utr;Parent=MsG0180002083.01.T01
Chr1 CDS 32409187 32409614 32409187 ID=MsG0180002083.01.T01:cds;Parent=MsG0180002083.01.T01
Chr1 CDS 32409824 32409868 32409824 ID=MsG0180002083.01.T01:cds;Parent=MsG0180002083.01.T01
Chr1 CDS 32411749 32411947 32411749 ID=MsG0180002083.01.T01:cds;Parent=MsG0180002083.01.T01
Gene Sequence

>MsG0180002083.01.T01

ATGAGTTCCAACACCAAACAAACTCAGTTTCCCCCTGAGGTTGAAAAAGTTGATGATGCTTATCTTTCTGCATTGTTACTATGTTTTAGTAGGATTTTTCCTGCAATACTAAATGCTGCTATTGATCTCAATTTATTTGATATCATAGCTAAGTTACAAAATTCAAGTGAACATTCTAGTTTTTCTGCTTCTGAAATTGCTTCTCAGCTTCCAAATCAACACCTTGAATTGGCTGAGAGGCTTGAACGCATGTTACCTGTGTTGGCTAGTTACTCTCTTCTCACTTGTTCCATTCGTACCAATGAAGATGGTAAGAGAGAAAGAGTTTATGCTCTCTCATCAATTGGTCAATACTTTGCATTGGATAAAGATGGAGGCTCTTTGGGTCTACTCTCAGCATTAATCCATCGTGGATATCATGATGTTTGGGAAGAAGATCCTAGTAATTTGAAATTTAATCGAGAGGTACCTAGGGGCAGAAGCCCTGTCAAAATGCAGAAAGCCTTGGCAAATGTACGTTTGCCAGGAGCTTTGCCAGGAGCAGAATCACTAGCAAATATGCCCATCGCAAATGAACATGCCAGGGCTTTCGCTCCTGGCAAATTCGCTCCTGGCAAAGACCCTGGCACAACTCCTGGCAAAATGCGTCTGATAGAACACAGACTTTTCTGA
Protein sequence

>MsG0180002083.01.T01

MSSNTKQTQFPPEVEKVDDAYLSALLLCFSRIFPAILNAAIDLNLFDIIAKLQNSSEHSSFSASEIASQLPNQHLELAERLERMLPVLASYSLLTCSIRTNEDGKRERVYALSSIGQYFALDKDGGSLGLLSALIHRGYHDVWEEDPSNLKFNREVPRGRSPVKMQKALANVRLPGALPGAESLANMPIANEHARAFAPGKFAPGKDPGTTPGKMRLIEHRLF*