AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0180002180.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180002180.01.T01 MTR_1g049100 90.404 198 16 2 1 196 1 197 1.33e-110 314
MsG0180002180.01.T01 MTR_5g036480 67.932 237 35 4 1 196 1 237 3.11e-90 264
MsG0180002180.01.T01 MTR_4g106590 59.669 181 66 3 3 179 21 198 1.20e-62 193
MsG0180002180.01.T01 MTR_3g078613 61.006 159 56 2 7 162 22 177 1.33e-60 187
MsG0180002180.01.T01 MTR_3g078613 60.625 160 56 3 7 162 22 178 8.93e-59 183
MsG0180002180.01.T01 MTR_7g490310 51.813 193 78 3 7 184 8 200 2.28e-56 178
MsG0180002180.01.T01 MTR_8g038620 59.155 142 53 2 7 143 8 149 8.91e-51 162
MsG0180002180.01.T01 MTR_3g093860 58.696 138 50 3 4 138 14 147 1.29e-49 158
MsG0180002180.01.T01 MTR_3g015490 52.903 155 61 3 7 152 3 154 1.09e-45 149
MsG0180002180.01.T01 MTR_3g088630 50.676 148 57 3 6 138 20 166 1.09e-42 143
MsG0180002180.01.T01 MTR_6g007460 41.622 185 52 4 7 138 10 191 2.83e-35 123
MsG0180002180.01.T01 MTR_4g061360 35.075 134 75 2 8 141 89 210 2.24e-17 79.7
MsG0180002180.01.T01 MTR_3g092780 29.932 147 88 2 8 151 56 190 5.72e-17 78.6
MsG0180002180.01.T01 MTR_3g092780 29.932 147 88 2 8 151 56 190 6.70e-17 78.6
MsG0180002180.01.T01 MTR_3g092780 29.730 148 88 2 8 151 56 191 1.11e-16 77.8
MsG0180002180.01.T01 MTR_3g092780 29.730 148 88 2 8 151 56 191 1.17e-16 77.8
MsG0180002180.01.T01 MTR_7g118260 31.757 148 87 3 14 161 22 155 6.54e-16 75.5
MsG0180002180.01.T01 MTR_4g051330 52.239 67 29 1 89 152 77 143 6.76e-15 69.3
MsG0180002180.01.T01 MTR_7g118260 32.308 130 74 3 14 143 22 137 2.13e-14 71.2
MsG0180002180.01.T01 MTR_8g024260 30.882 136 82 1 8 143 36 159 5.27e-14 70.1
MsG0180002180.01.T01 MTR_1g067110 28.788 132 82 1 10 141 87 206 2.42e-13 68.2
MsG0180002180.01.T01 MTR_1g067110 28.788 132 82 1 10 141 87 206 2.92e-13 67.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180002180.01.T01 AT1G59940 59.901 202 60 5 4 188 29 226 7.38e-73 220
MsG0180002180.01.T01 AT1G59940 59.901 202 60 5 4 188 121 318 1.85e-71 219
MsG0180002180.01.T01 AT1G10470 55.752 226 57 6 6 192 18 239 5.01e-70 213
MsG0180002180.01.T01 AT1G10470 55.752 226 57 6 6 192 32 253 1.11e-69 213
MsG0180002180.01.T01 AT3G48100 65.563 151 50 1 8 158 25 173 1.15e-63 195
MsG0180002180.01.T01 AT5G62920 72.656 128 32 1 8 135 25 149 4.41e-61 188
MsG0180002180.01.T01 AT1G74890 71.318 129 34 1 7 135 17 142 3.67e-59 184
MsG0180002180.01.T01 AT1G19050 65.441 136 44 1 7 142 23 155 2.50e-58 182
MsG0180002180.01.T01 AT2G40670 59.259 135 55 0 4 138 25 159 1.76e-54 171
MsG0180002180.01.T01 AT3G57040 58.993 139 50 2 7 138 8 146 2.65e-53 170
MsG0180002180.01.T01 AT3G57040 58.993 139 50 2 7 138 8 146 2.65e-53 170
MsG0180002180.01.T01 AT3G56380 61.194 134 48 2 6 138 18 148 2.67e-53 167
MsG0180002180.01.T01 AT2G41310 59.441 143 52 2 1 138 3 144 6.57e-53 169
MsG0180002180.01.T01 AT2G40670 58.824 136 55 1 4 138 25 160 9.09e-53 167
MsG0180002180.01.T01 AT3G56380 60.741 135 48 3 6 138 18 149 1.77e-51 163
MsG0180002180.01.T01 AT1G10470 56.044 91 30 6 70 155 17 102 2.02e-23 92.4
MsG0180002180.01.T01 AT2G46790 31.343 134 80 2 8 141 37 158 2.46e-15 73.9
MsG0180002180.01.T01 AT5G24470 30.216 139 83 3 8 146 52 176 2.54e-15 73.9
MsG0180002180.01.T01 AT2G46790 31.343 134 80 2 8 141 37 158 3.36e-15 73.6
MsG0180002180.01.T01 AT5G60100 34.848 132 74 2 10 141 66 185 2.14e-14 70.9
MsG0180002180.01.T01 AT5G60100 34.848 132 74 2 10 141 66 185 2.14e-14 70.9
MsG0180002180.01.T01 AT5G60100 34.848 132 74 2 10 141 66 185 2.14e-14 70.9
MsG0180002180.01.T01 AT5G60100 34.848 132 74 2 10 141 66 185 3.27e-14 70.5
MsG0180002180.01.T01 AT5G60100 34.848 132 74 2 10 141 66 185 3.42e-14 70.5
MsG0180002180.01.T01 AT5G60100 34.848 132 74 2 10 141 66 185 3.42e-14 70.5
MsG0180002180.01.T01 AT5G60100 34.848 132 74 2 10 141 66 185 3.42e-14 70.5
MsG0180002180.01.T01 AT2G07440 60.000 50 20 0 89 138 9 58 2.13e-13 64.7
MsG0180002180.01.T01 AT5G02810 28.358 134 84 1 8 141 78 199 1.09e-12 66.2

Find 38 sgRNAs with CRISPR-Local

Find 61 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GAGTGATTACATGCTTCTTC+TGG 0.290924 1:+34335185 None:intergenic
ATTGAAAATATTCAGATGTT+TGG 0.307010 1:-34335321 MsG0180002180.01.T01:intron
AACTCATAACCAGTCATTTC+TGG 0.328147 1:+34336057 None:intergenic
TTTGTTGCCTTAGCCTTTGA+AGG 0.366124 1:-34336109 MsG0180002180.01.T01:intron
CTCAAAATTTCTGCTTGTAA+AGG 0.386939 1:-34336480 MsG0180002180.01.T01:intron
TTCGGAAGAGGTTCATGTAT+TGG 0.417794 1:-34336547 MsG0180002180.01.T01:CDS
AGAAGTGATGGAGACAAATT+CGG 0.432688 1:-34336565 None:intergenic
TTAGTTACTGCTGTGGATAG+TGG 0.438893 1:-34336288 MsG0180002180.01.T01:intron
TTCACATCACATAACTTCAC+TGG 0.475573 1:+34335269 None:intergenic
ATAACAGTACCTGTCTATGC+GGG 0.487586 1:+34335849 None:intergenic
TCAGAAGACATAATCACTAC+TGG 0.489512 1:+34335883 None:intergenic
ATGTGAAGGTGATGATGAAA+TGG 0.496627 1:+34335148 None:intergenic
ATATTCTGCCCCGCATAGAC+AGG 0.510615 1:-34335858 MsG0180002180.01.T01:intron
ATATTCAGATGTTTGGAAGA+AGG 0.511460 1:-34335314 MsG0180002180.01.T01:intron
TATTGCATGCCAGAAATGAC+TGG 0.520879 1:-34336066 MsG0180002180.01.T01:CDS
TTGATCGGAAAGTTATTGAA+AGG 0.524082 1:-34336506 MsG0180002180.01.T01:CDS
ATAACAAGATCCACCTTCAA+AGG 0.539532 1:+34336096 None:intergenic
GTTGCCTTAGCCTTTGAAGG+TGG 0.539999 1:-34336106 MsG0180002180.01.T01:intron
TGCTTGATTCTCCAATCAGA+CGG 0.554606 1:-34335097 MsG0180002180.01.T01:CDS
TACATGACAACAAGGGAGGT+TGG 0.562595 1:-34335233 MsG0180002180.01.T01:CDS
AGCACACTGGATGCAGACAA+TGG 0.577593 1:+34335116 None:intergenic
GATGGAGACAAATTCGGAAG+AGG 0.584900 1:-34336559 MsG0180002180.01.T01:CDS
ACAACAAGGGAGGTTGGAGT+AGG 0.598300 1:-34335227 MsG0180002180.01.T01:CDS
ATGTTTGGAAGAAGGTGCAA+AGG 0.598645 1:-34335306 MsG0180002180.01.T01:CDS
AGAGAGAAGTCTGAATCTGA+TGG 0.604808 1:-34336228 MsG0180002180.01.T01:CDS
GTGAACTTTAGTTACTGCTG+TGG 0.606977 1:-34336295 MsG0180002180.01.T01:intron
TTGATGATAGTCTCATTGAT+CGG 0.612021 1:-34336521 MsG0180002180.01.T01:CDS
TGAAGGGTTACATGACAACA+AGG 0.633631 1:-34335241 MsG0180002180.01.T01:CDS
GATTGGAGAATCAAGCACAC+TGG 0.650465 1:+34335103 None:intergenic
ATGTGATGTGAAACGACTGA+AGG 0.663812 1:-34335258 MsG0180002180.01.T01:CDS
TGTGATGTGAAACGACTGAA+GGG 0.670059 1:-34335257 MsG0180002180.01.T01:CDS
TATAACAGTACCTGTCTATG+CGG 0.670864 1:+34335848 None:intergenic
AATGGAGATGATGAATGTGA+AGG 0.695750 1:+34335134 None:intergenic
TAACAGTACCTGTCTATGCG+GGG 0.697429 1:+34335850 None:intergenic
GGTGATGATGAAATGGACGG+TGG 0.704627 1:+34335155 None:intergenic
GGGTTACATGACAACAAGGG+AGG 0.715675 1:-34335237 MsG0180002180.01.T01:CDS
GAAGGTGATGATGAAATGGA+CGG 0.719690 1:+34335152 None:intergenic
GAAGGGTTACATGACAACAA+GGG 0.758159 1:-34335240 MsG0180002180.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TATAGTAATGATTTTTTCAA+TGG + Chr1:34335638-34335657 None:intergenic 15.0%
!! ATTGAAAATATTCAGATGTT+TGG - Chr1:34336310-34336329 MsG0180002180.01.T01:intron 20.0%
!!! ACGAAATAGATGTTTTTAAA+GGG - Chr1:34335949-34335968 MsG0180002180.01.T01:intron 20.0%
!!! ATTTAACTGTCTTTTTTCTT+TGG + Chr1:34336105-34336124 None:intergenic 20.0%
!!! TACGAAATAGATGTTTTTAA+AGG - Chr1:34335948-34335967 MsG0180002180.01.T01:intron 20.0%
! TGAGTTGCTCAAAAAAATTA+AGG - Chr1:34335591-34335610 MsG0180002180.01.T01:intron 25.0%
!! GTCATTAAAAAGTATAACCA+TGG + Chr1:34336017-34336036 None:intergenic 25.0%
!! TTATTGTTATTAGTTACCAC+TGG - Chr1:34336248-34336267 MsG0180002180.01.T01:CDS 25.0%
!! TTTTCAATTGTAACAAACCA+TGG - Chr1:34335997-34336016 MsG0180002180.01.T01:intron 25.0%
!!! CGAAATAGATGTTTTTAAAG+GGG - Chr1:34335950-34335969 MsG0180002180.01.T01:intron 25.0%
!!! GTGTTGATGATTTTTGTTTT+TGG - Chr1:34335314-34335333 MsG0180002180.01.T01:intron 25.0%
ATATTCAGATGTTTGGAAGA+AGG - Chr1:34336317-34336336 MsG0180002180.01.T01:intron 30.0%
CCTGCTTCAAAATGAAATTT+CGG + Chr1:34335705-34335724 None:intergenic 30.0%
CTCAAAATTTCTGCTTGTAA+AGG - Chr1:34335151-34335170 MsG0180002180.01.T01:CDS 30.0%
TTGATCGGAAAGTTATTGAA+AGG - Chr1:34335125-34335144 MsG0180002180.01.T01:CDS 30.0%
! ATCATTTTAAACCGTCTGAT+TGG + Chr1:34336548-34336567 None:intergenic 30.0%
! TTGATGATAGTCTCATTGAT+CGG - Chr1:34335110-34335129 MsG0180002180.01.T01:CDS 30.0%
AACTCATAACCAGTCATTTC+TGG + Chr1:34335577-34335596 None:intergenic 35.0%
AACTTCATCACAATTTCCAG+TGG + Chr1:34336267-34336286 None:intergenic 35.0%
AATGGAGATGATGAATGTGA+AGG + Chr1:34336500-34336519 None:intergenic 35.0%
ATAACAAGATCCACCTTCAA+AGG + Chr1:34335538-34335557 None:intergenic 35.0%
TATAACAGTACCTGTCTATG+CGG + Chr1:34335786-34335805 None:intergenic 35.0%
TCAGAAGACATAATCACTAC+TGG + Chr1:34335751-34335770 None:intergenic 35.0%
TTCACATCACATAACTTCAC+TGG + Chr1:34336365-34336384 None:intergenic 35.0%
! ATGTGAAGGTGATGATGAAA+TGG + Chr1:34336486-34336505 None:intergenic 35.0%
! TCTGATGGTTCTATTGTATG+TGG - Chr1:34335418-34335437 MsG0180002180.01.T01:intron 35.0%
!!! AATCAGAGCACTGCAATTTT+TGG - Chr1:34335366-34335385 MsG0180002180.01.T01:intron 35.0%
!!! ATCAGAGCACTGCAATTTTT+GGG - Chr1:34335367-34335386 MsG0180002180.01.T01:intron 35.0%
!!! CCGAAATTTCATTTTGAAGC+AGG - Chr1:34335702-34335721 MsG0180002180.01.T01:intron 35.0%
AGAGAGAAGTCTGAATCTGA+TGG - Chr1:34335403-34335422 MsG0180002180.01.T01:intron 40.0%
ATAACAGTACCTGTCTATGC+GGG + Chr1:34335785-34335804 None:intergenic 40.0%
ATGTGATGTGAAACGACTGA+AGG - Chr1:34336373-34336392 MsG0180002180.01.T01:intron 40.0%
GAAGGGTTACATGACAACAA+GGG - Chr1:34336391-34336410 MsG0180002180.01.T01:intron 40.0%
GAGTGATTACATGCTTCTTC+TGG + Chr1:34336449-34336468 None:intergenic 40.0%
GTAAAGGTGAGTTTGTGATC+TGG - Chr1:34335167-34335186 MsG0180002180.01.T01:CDS 40.0%
GTGAACTTTAGTTACTGCTG+TGG - Chr1:34335336-34335355 MsG0180002180.01.T01:intron 40.0%
TATTGCATGCCAGAAATGAC+TGG - Chr1:34335565-34335584 MsG0180002180.01.T01:intron 40.0%
TGAAGGGTTACATGACAACA+AGG - Chr1:34336390-34336409 MsG0180002180.01.T01:intron 40.0%
TGCTTGATTCTCCAATCAGA+CGG - Chr1:34336534-34336553 MsG0180002180.01.T01:CDS 40.0%
TGTGATGTGAAACGACTGAA+GGG - Chr1:34336374-34336393 MsG0180002180.01.T01:intron 40.0%
TTAGTTACTGCTGTGGATAG+TGG - Chr1:34335343-34335362 MsG0180002180.01.T01:intron 40.0%
TTCGGAAGAGGTTCATGTAT+TGG - Chr1:34335084-34335103 MsG0180002180.01.T01:CDS 40.0%
! ATGTTTGGAAGAAGGTGCAA+AGG - Chr1:34336325-34336344 MsG0180002180.01.T01:intron 40.0%
! GAAGGTGATGATGAAATGGA+CGG + Chr1:34336482-34336501 None:intergenic 40.0%
! GCAGGAATCGTCAACTTTTA+AGG - Chr1:34335720-34335739 MsG0180002180.01.T01:intron 40.0%
! TTTGTTGCCTTAGCCTTTGA+AGG - Chr1:34335522-34335541 MsG0180002180.01.T01:intron 40.0%
!!! TCAGAGCACTGCAATTTTTG+GGG - Chr1:34335368-34335387 MsG0180002180.01.T01:intron 40.0%
AGATCCACCTTCAAAGGCTA+AGG + Chr1:34335532-34335551 None:intergenic 45.0%
GATTGGAGAATCAAGCACAC+TGG + Chr1:34336531-34336550 None:intergenic 45.0%
TAACAGTACCTGTCTATGCG+GGG + Chr1:34335784-34335803 None:intergenic 45.0%
TACATGACAACAAGGGAGGT+TGG - Chr1:34336398-34336417 MsG0180002180.01.T01:intron 45.0%
!!! AGCACTGCAATTTTTGGGGT+TGG - Chr1:34335372-34335391 MsG0180002180.01.T01:intron 45.0%
!! TAATTTATACTTATATATAA+TGG + Chr1:34336139-34336158 None:intergenic 5.0%
!!! ATTTTTTTTAAACTTTTTTA+AGG - Chr1:34335805-34335824 MsG0180002180.01.T01:intron 5.0%
!!! TTTTTTTTAAACTTTTTTAA+GGG - Chr1:34335806-34335825 MsG0180002180.01.T01:intron 5.0%
ACAACAAGGGAGGTTGGAGT+AGG - Chr1:34336404-34336423 MsG0180002180.01.T01:intron 50.0%
AGCACACTGGATGCAGACAA+TGG + Chr1:34336518-34336537 None:intergenic 50.0%
ATATTCTGCCCCGCATAGAC+AGG - Chr1:34335773-34335792 MsG0180002180.01.T01:intron 50.0%
GGGTTACATGACAACAAGGG+AGG - Chr1:34336394-34336413 MsG0180002180.01.T01:intron 50.0%
! GGTGATGATGAAATGGACGG+TGG + Chr1:34336479-34336498 None:intergenic 50.0%
! GTTGCCTTAGCCTTTGAAGG+TGG - Chr1:34335525-34335544 MsG0180002180.01.T01:intron 50.0%
Chromosome Type Strat End Strand Name
Chr1 gene 34335073 34336580 34335073 ID=MsG0180002180.01;Name=MsG0180002180.01
Chr1 mRNA 34335073 34336580 34335073 ID=MsG0180002180.01.T01;Parent=MsG0180002180.01;Name=MsG0180002180.01.T01;_AED=0.44;_eAED=0.44;_QI=0|0|0|1|1|1|5|0|196
Chr1 exon 34336481 34336580 34336481 ID=MsG0180002180.01.T01:exon:6888;Parent=MsG0180002180.01.T01
Chr1 exon 34336221 34336306 34336221 ID=MsG0180002180.01.T01:exon:6889;Parent=MsG0180002180.01.T01
Chr1 exon 34336041 34336118 34336041 ID=MsG0180002180.01.T01:exon:6890;Parent=MsG0180002180.01.T01
Chr1 exon 34335859 34335929 34335859 ID=MsG0180002180.01.T01:exon:6891;Parent=MsG0180002180.01.T01
Chr1 exon 34335073 34335328 34335073 ID=MsG0180002180.01.T01:exon:6892;Parent=MsG0180002180.01.T01
Chr1 CDS 34336481 34336580 34336481 ID=MsG0180002180.01.T01:cds;Parent=MsG0180002180.01.T01
Chr1 CDS 34336221 34336306 34336221 ID=MsG0180002180.01.T01:cds;Parent=MsG0180002180.01.T01
Chr1 CDS 34336041 34336118 34336041 ID=MsG0180002180.01.T01:cds;Parent=MsG0180002180.01.T01
Chr1 CDS 34335859 34335929 34335859 ID=MsG0180002180.01.T01:cds;Parent=MsG0180002180.01.T01
Chr1 CDS 34335073 34335328 34335073 ID=MsG0180002180.01.T01:cds;Parent=MsG0180002180.01.T01
Gene Sequence

>MsG0180002180.01.T01

ATGGAGACAAATTCGGAAGAGGTTCATGTATTGGCTGTTGATGATAGTCTCATTGATCGGAAAGTTATTGAAAGGTTGCTCAAAATTTCTGCTTGTAAAGTTACTGCTGTGGATAGTGGAATCAGAGCACTGCAATTTTTGGGGTTGGATGAGCAGAGAGAGAAGTCTGAATCTGATGGTTCTATTCCTTTGAAGGTGGATCTTGTTATTACAGATTATTGCATGCCAGAAATGACTGGTTATGAGTTGCTCAAAAAAATTAAGGAATCGTCAACTTTTAAGGAAATTCCAGTAGTGATTATGTCTTCTGAAAATATTCTGCCCCGCATAGACAGATGTTTGGAAGAAGGTGCAAAGGATTTTATAATAAAGCCAGTGAAGTTATGTGATGTGAAACGACTGAAGGGTTACATGACAACAAGGGAGGTTGGAGTAGGAATCAACAACAAACAGAAGCCAGAAGAAGCATGTAATCACTCATCATCACCACCGTCCATTTCATCATCACCTTCACATTCATCATCTCCATTGTCTGCATCCAGTGTGCTTGATTCTCCAATCAGACGGTTTAAAATGATTGACACTGAATGA

Protein sequence

>MsG0180002180.01.T01

METNSEEVHVLAVDDSLIDRKVIERLLKISACKVTAVDSGIRALQFLGLDEQREKSESDGSIPLKVDLVITDYCMPEMTGYELLKKIKESSTFKEIPVVIMSSENILPRIDRCLEEGAKDFIIKPVKLCDVKRLKGYMTTREVGVGINNKQKPEEACNHSSSPPSISSSPSHSSSPLSASSVLDSPIRRFKMIDTE*