Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680030351.01.T01 | XP_013450761.1 | 92.903 | 155 | 3 | 2 | 1 | 147 | 165 | 319 | 3.14E-59 | 202 |
| MsG0680030351.01.T01 | XP_013450761.1 | 95.556 | 45 | 2 | 0 | 213 | 257 | 1 | 45 | 1.35E-18 | 95.5 |
| MsG0680030351.01.T02 | XP_013450761.1 | 92.903 | 155 | 3 | 2 | 1 | 147 | 165 | 319 | 3.14E-59 | 202 |
| MsG0680030351.01.T02 | XP_013450761.1 | 95.556 | 45 | 2 | 0 | 213 | 257 | 1 | 45 | 1.35E-18 | 95.5 |
| MsG0680030351.01.T03 | XP_013450761.1 | 81.065 | 169 | 7 | 2 | 1 | 167 | 1 | 146 | 3.44E-85 | 265 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680030351.01.T01 | A0A072U4W5 | 92.903 | 155 | 3 | 2 | 1 | 147 | 165 | 319 | 1.50e-59 | 202 |
| MsG0680030351.01.T02 | A0A072U4W5 | 92.903 | 155 | 3 | 2 | 1 | 147 | 165 | 319 | 1.50e-59 | 202 |
| MsG0680030351.01.T03 | A0A072U4W5 | 81.065 | 169 | 7 | 2 | 1 | 167 | 1 | 146 | 1.64e-85 | 265 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080047825.01 | MsG0680030351.01 | 0.809488 | 1.845754e-50 | 1.085118e-47 |
| MsG0080047959.01 | MsG0680030351.01 | 0.805122 | 1.554940e-49 | 8.159949e-47 |
| MsG0080048178.01 | MsG0680030351.01 | 0.809102 | 2.233152e-50 | 1.299486e-47 |
| MsG0080048322.01 | MsG0680030351.01 | 0.803484 | 3.410836e-49 | 1.716064e-46 |
| MsG0080048765.01 | MsG0680030351.01 | 0.801250 | 9.837870e-49 | 4.676065e-46 |
| MsG0080048785.01 | MsG0680030351.01 | 0.823987 | 1.030032e-53 | 9.007435e-51 |
| MsG0080048830.01 | MsG0680030351.01 | 0.839716 | 1.341240e-57 | 1.868015e-54 |
| MsG0080048856.01 | MsG0680030351.01 | 0.800903 | 1.158623e-48 | 5.459156e-46 |
| MsG0080048964.01 | MsG0680030351.01 | 0.819170 | 1.336924e-52 | 1.021129e-49 |
| MsG0080048968.01 | MsG0680030351.01 | 0.808735 | 2.676135e-50 | 1.541958e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|---|---|
| MsG0680030351.01 | MsG0180002532.01 | PPI |
| MsG0680030351.01 | MsG0180002180.01 | PPI |
| MsG0680030351.01 | MsG0580025893.01 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680030351.01.T01 | MTR_6g004810 | 92.903 | 155 | 3 | 2 | 1 | 147 | 165 | 319 | 3.80e-63 | 202 |
| MsG0680030351.01.T01 | MTR_6g004810 | 95.556 | 45 | 2 | 0 | 213 | 257 | 1 | 45 | 1.63e-22 | 95.5 |
| MsG0680030351.01.T01 | MTR_3g082650 | 52.475 | 101 | 43 | 3 | 1 | 96 | 138 | 238 | 1.39e-17 | 81.3 |
| MsG0680030351.01.T01 | MTR_5g097580 | 40.351 | 114 | 47 | 4 | 1 | 94 | 138 | 250 | 2.81e-11 | 63.5 |
| MsG0680030351.01.T01 | MTR_5g097580 | 40.351 | 114 | 47 | 4 | 1 | 94 | 138 | 250 | 2.82e-11 | 63.5 |
| MsG0680030351.01.T02 | MTR_6g004810 | 92.903 | 155 | 3 | 2 | 1 | 147 | 165 | 319 | 3.80e-63 | 202 |
| MsG0680030351.01.T02 | MTR_6g004810 | 95.556 | 45 | 2 | 0 | 213 | 257 | 1 | 45 | 1.63e-22 | 95.5 |
| MsG0680030351.01.T02 | MTR_3g082650 | 52.475 | 101 | 43 | 3 | 1 | 96 | 138 | 238 | 1.39e-17 | 81.3 |
| MsG0680030351.01.T02 | MTR_5g097580 | 40.351 | 114 | 47 | 4 | 1 | 94 | 138 | 250 | 2.81e-11 | 63.5 |
| MsG0680030351.01.T02 | MTR_5g097580 | 40.351 | 114 | 47 | 4 | 1 | 94 | 138 | 250 | 2.82e-11 | 63.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0680030351.01.T01 | AT5G40490 | 67.164 | 67 | 22 | 0 | 1 | 67 | 145 | 211 | 7.18e-23 | 96.7 |
| MsG0680030351.01.T01 | AT5G40490 | 67.164 | 67 | 22 | 0 | 1 | 67 | 162 | 228 | 1.04e-22 | 96.7 |
| MsG0680030351.01.T01 | AT3G13224 | 67.164 | 67 | 21 | 1 | 1 | 66 | 141 | 207 | 4.33e-20 | 88.6 |
| MsG0680030351.01.T01 | AT3G13224 | 67.164 | 67 | 21 | 1 | 1 | 66 | 132 | 198 | 5.91e-20 | 88.2 |
| MsG0680030351.01.T01 | AT3G13224 | 68.182 | 44 | 14 | 0 | 1 | 44 | 141 | 184 | 4.65e-13 | 67.0 |
| MsG0680030351.01.T01 | AT1G17640 | 48.148 | 81 | 34 | 1 | 1 | 81 | 189 | 261 | 2.91e-12 | 66.2 |
| MsG0680030351.01.T01 | AT1G17640 | 48.148 | 81 | 34 | 1 | 1 | 81 | 189 | 261 | 2.91e-12 | 66.2 |
| MsG0680030351.01.T01 | AT1G17640 | 48.148 | 81 | 34 | 1 | 1 | 81 | 189 | 261 | 2.91e-12 | 66.2 |
| MsG0680030351.01.T02 | AT5G40490 | 67.164 | 67 | 22 | 0 | 1 | 67 | 145 | 211 | 7.18e-23 | 96.7 |
| MsG0680030351.01.T02 | AT5G40490 | 67.164 | 67 | 22 | 0 | 1 | 67 | 162 | 228 | 1.04e-22 | 96.7 |
Find 124 sgRNAs with CRISPR-Local
Find 777 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AACAGCTTCCTCAGTGTCAA+AGG | 0.539442 | 6:+970957 | None:intergenic |
| AAGGGGAGCTGTTGGCTCTA+AGG | 0.366354 | 6:-971649 | MsG0680030351.01.T02:five_prime_UTR |
| AATGACTCAAGGTCTTCATA+CGG | 0.631517 | 6:-969842 | MsG0680030351.01.T02:CDS |
| ACAAAGAAAATATTTGTAGG+TGG | 0.622507 | 6:-971618 | MsG0680030351.01.T02:five_prime_UTR |
| ACCAAAGTGCTTGATAAATT+GGG | 0.384066 | 6:+971958 | None:intergenic |
| ACTAATCGTTCCCGTGGCTT+TGG | 0.284542 | 6:-970988 | MsG0680030351.01.T02:CDS |
| ACTATACCAAGGGGAGCTGT+TGG | 0.556055 | 6:-971657 | MsG0680030351.01.T02:five_prime_UTR |
| ACTGGACAGCCTCGTGGATT+TGG | 0.238397 | 6:-971890 | MsG0680030351.01.T02:five_prime_UTR |
| AGAGAAGGATCTCCTCTGTA+GGG | 0.575562 | 6:+969665 | None:intergenic |
| AGATTAAGCGGACTATACCA+AGG | 0.658644 | 6:-971668 | MsG0680030351.01.T02:five_prime_UTR |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAAATAATGATTCGTCCTGT+TGG | + | Chr6:964639-964658 | None:intergenic | 30.0% | |
| AAACAACATCAACAAAAGCA+AGG | + | Chr6:962705-962724 | None:intergenic | 30.0% | |
| AAATAATGATTCGTCCTGTT+GGG | + | Chr6:964638-964657 | None:intergenic | 30.0% | |
| AAATATGCTGAAGCTACTAT+AGG | - | Chr6:963512-963531 | MsG0680030351.01.T03:intron | 30.0% | |
| AAATTGGGCAAAAAAAAAGG+GGG | + | Chr6:964806-964825 | None:intergenic | 30.0% | |
| AACTCTTCCATAAGTTTAAG+GGG | - | Chr6:963187-963206 | MsG0680030351.01.T03:intron | 30.0% | |
| AAGAAATTGAACACAACCTA+TGG | - | Chr6:966324-966343 | MsG0680030351.01.T02:intron | 30.0% | |
| AAGCAAAAACAAGGTTCAAA+AGG | - | Chr6:965437-965456 | MsG0680030351.01.T02:intron | 30.0% | |
| AATAGATAAAGCCCAAGAAA+CGG | - | Chr6:969500-969519 | MsG0680030351.01.T02:intron | 30.0% | |
| AATCATCATTGGGGTATAAA+AGG | - | Chr6:964569-964588 | MsG0680030351.01.T02:intron | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr6 | gene | 960651 | 973176 | 960651 | ID=MsG0680030351.01;Name=MsG0680030351.01 |
| Chr6 | mRNA | 963971 | 973176 | 963971 | ID=MsG0680030351.01.T01;Parent=MsG0680030351.01;Name=MsG0680030351.01.T01;_AED=0.49;_eAED=0.49;_QI=619|0.62|0.55|1|0.62|0.55|9|0|257 |
| Chr6 | exon | 970909 | 971078 | 970909 | ID=MsG0680030351.01.T01:exon:2337;Parent=MsG0680030351.01.T01 |
| Chr6 | exon | 971595 | 971694 | 971595 | ID=MsG0680030351.01.T01:exon:2336;Parent=MsG0680030351.01.T01 |
| Chr6 | five_prime_UTR | 972915 | 973176 | 972915 | ID=MsG0680030351.01.T01:five_prime_utr;Parent=MsG0680030351.01.T01 |
| Chr6 | five_prime_UTR | 972125 | 972165 | 972125 | ID=MsG0680030351.01.T01:five_prime_utr;Parent=MsG0680030351.01.T01 |
| Chr6 | five_prime_UTR | 971818 | 971980 | 971818 | ID=MsG0680030351.01.T01:five_prime_utr;Parent=MsG0680030351.01.T01 |
| Chr6 | five_prime_UTR | 971595 | 971694 | 971595 | ID=MsG0680030351.01.T01:five_prime_utr;Parent=MsG0680030351.01.T01 |
| Chr6 | five_prime_UTR | 971026 | 971078 | 971026 | ID=MsG0680030351.01.T01:five_prime_utr;Parent=MsG0680030351.01.T01 |
| Chr6 | CDS | 970909 | 971025 | 970909 | ID=MsG0680030351.01.T01:cds;Parent=MsG0680030351.01.T01 |
| Chr6 | CDS | 969555 | 969927 | 969555 | ID=MsG0680030351.01.T01:cds;Parent=MsG0680030351.01.T01 |
| Chr6 | CDS | 968354 | 968410 | 968354 | ID=MsG0680030351.01.T01:cds;Parent=MsG0680030351.01.T01 |
| Chr6 | CDS | 966243 | 966322 | 966243 | ID=MsG0680030351.01.T01:cds;Parent=MsG0680030351.01.T01 |
| Chr6 | CDS | 963971 | 964117 | 963971 | ID=MsG0680030351.01.T01:cds;Parent=MsG0680030351.01.T01 |
| Chr6 | mRNA | 963971 | 973176 | 963971 | ID=MsG0680030351.01.T02;Parent=MsG0680030351.01;Name=MsG0680030351.01.T02;_AED=0.49;_eAED=0.49;_QI=1135|0.57|0.5|1|0.71|0.62|8|0|257 |
| Chr6 | five_prime_UTR | 972915 | 973176 | 972915 | ID=MsG0680030351.01.T02:five_prime_utr;Parent=MsG0680030351.01.T02 |
| Chr6 | five_prime_UTR | 972125 | 972165 | 972125 | ID=MsG0680030351.01.T02:five_prime_utr;Parent=MsG0680030351.01.T02 |
| Chr6 | five_prime_UTR | 971818 | 971980 | 971818 | ID=MsG0680030351.01.T02:five_prime_utr;Parent=MsG0680030351.01.T02 |
| Chr6 | five_prime_UTR | 971026 | 971694 | 971026 | ID=MsG0680030351.01.T02:five_prime_utr;Parent=MsG0680030351.01.T02 |
| Chr6 | CDS | 970909 | 971025 | 970909 | ID=MsG0680030351.01.T02:cds;Parent=MsG0680030351.01.T02 |
| Chr6 | CDS | 969555 | 969927 | 969555 | ID=MsG0680030351.01.T02:cds;Parent=MsG0680030351.01.T02 |
| Chr6 | CDS | 968354 | 968410 | 968354 | ID=MsG0680030351.01.T02:cds;Parent=MsG0680030351.01.T02 |
| Chr6 | CDS | 966243 | 966322 | 966243 | ID=MsG0680030351.01.T02:cds;Parent=MsG0680030351.01.T02 |
| Chr6 | CDS | 963971 | 964117 | 963971 | ID=MsG0680030351.01.T02:cds;Parent=MsG0680030351.01.T02 |
| Chr6 | mRNA | 960651 | 973176 | 960651 | ID=MsG0680030351.01.T03;Parent=MsG0680030351.01;Name=MsG0680030351.01.T03;_AED=0.49;_eAED=0.51;_QI=128|0.8|0.83|1|0.6|0.33|6|1161|172 |
| Chr6 | exon | 960651 | 960748 | 960651 | ID=MsG0680030351.01.T03:exon:2345;Parent=MsG0680030351.01.T03 |
| Chr6 | exon | 969534 | 969927 | 969534 | ID=MsG0680030351.01.T03:exon:2344;Parent=MsG0680030351.01.T03 |
| Chr6 | exon | 971754 | 971980 | 971754 | ID=MsG0680030351.01.T03:exon:2343;Parent=MsG0680030351.01.T03 |
| Chr6 | five_prime_UTR | 973049 | 973176 | 973049 | ID=MsG0680030351.01.T03:five_prime_utr;Parent=MsG0680030351.01.T03 |
| Chr6 | CDS | 972915 | 973048 | 972915 | ID=MsG0680030351.01.T03:cds;Parent=MsG0680030351.01.T03 |
| Chr6 | CDS | 972125 | 972165 | 972125 | ID=MsG0680030351.01.T03:cds;Parent=MsG0680030351.01.T03 |
| Chr6 | CDS | 971754 | 971980 | 971754 | ID=MsG0680030351.01.T03:cds;Parent=MsG0680030351.01.T03 |
| Chr6 | CDS | 971578 | 971694 | 971578 | ID=MsG0680030351.01.T03:cds;Parent=MsG0680030351.01.T03 |
| Chr6 | three_prime_UTR | 970909 | 971577 | 970909 | ID=MsG0680030351.01.T03:three_prime_utr;Parent=MsG0680030351.01.T03 |
| Chr6 | three_prime_UTR | 969534 | 969927 | 969534 | ID=MsG0680030351.01.T03:three_prime_utr;Parent=MsG0680030351.01.T03 |
| Chr6 | three_prime_UTR | 960651 | 960748 | 960651 | ID=MsG0680030351.01.T03:three_prime_utr;Parent=MsG0680030351.01.T03 |
| Gene Sequence |
>MsG0680030351.01.T01 ATGCGTGACCACTCTACTAATCGTTCCCGTGGCTTTGGGTTTGTCACCTTTGACACTGAGGAAGCTGTTGATGATCTTCTATCTATGGGGAACAAGATTGAGTTTGCTGGCACTCAGGTGGAAATCAAGAAGGCTGAACCAAAGAAGGCAAATGCACCACCTCCATCATCCAAGCGCTACAATGACTCAAGGTCTTCATACGGCAGTGGTGGGTATGGAGATGCTTATGATGGATTTGGTGGTGTTGGGGGTGGTTACAGCAGGTCTGGCAGTGCTTATGGTGGTAGGGGTGGTCCGTATGGTGGCTATGGAAGTGAGTTTGCTGGGTCTGGTGGATATGCTGGAGCAATGGGGGGACCCTACAGAGGAGATCCTTCTCTAGCATATGCAGGTCGTTATGGCGGAAGCTATGATCCTAGTGGGTATGGTGGACTTGGTGAGAGTTATGGGGCATATGGTGGTGCCGGTGCAGGTGCTGCTGCTGGCGGAGGTGTCGATTCTGAAAATATAAAGCAAAAACAAGGTTCAAAAGGTTGTGTATGTGCAGATGTCAACAAAAAAGCAAATGAAACAGATGTTAGAAAGGCAAGAAAAGCTGAACTCCAAAGAGCTGAAGCAACTGGGTTAAATCAATCAATGGAATCACCCAAAAACGATCACATCATTGGAACCGGAGGTGAACAAAACGACGTCGGTAGAAACCATGAACACGAACATGAACAACAACATCATAACTCTCAATCCCTTACCGGTGATGGTGCTAGTCCTGGGTAA >MsG0680030351.01.T03 ATGGAATCACCCAAAAACGATCACATCATTGGAACCGGAGGTGAACAAAACGACGTCGGTAGAAACCATGAACACGAACATGAACAACAACATCATAACTCTCAATCCCTTACCGGTGATGGTGCTAGTCCTGGAAAGATATTTATAGGAGGTTTAGCGAGAGAAACAACCATTGCCCAATTTATCAAGCACTTTGGTAAATATGGTGAGATTACCGATTCCGTCATAATGAAGGACAGGAAAACTGGACAGCCTCGTGGATTTGGGTTTATTACTTACGCTGATCCATCTGTGGTTGATAAAGTCATCGAGACTCTCATATCATCAACAGCAAACAAGTATGACTGTTTTTTTCTACCATGTCTTTGCATAGGATTTTACTCGTTTTGTTTTGATTATATAGTTGAGATTAAGCGGACTATACCAAGGGGAGCTGTTGGCTCTAAGGATTTTAGGACAAAGAAAATATTTGTAGGTGGTATTCCTTCAAATGTGACTGAAGGTAGGTTATCTGTTTAG >MsG0680030351.01.T02 ATGCGTGACCACTCTACTAATCGTTCCCGTGGCTTTGGGTTTGTCACCTTTGACACTGAGGAAGCTGTTGATGATCTTCTATCTATGGGGAACAAGATTGAGTTTGCTGGCACTCAGGTGGAAATCAAGAAGGCTGAACCAAAGAAGGCAAATGCACCACCTCCATCATCCAAGCGCTACAATGACTCAAGGTCTTCATACGGCAGTGGTGGGTATGGAGATGCTTATGATGGATTTGGTGGTGTTGGGGGTGGTTACAGCAGGTCTGGCAGTGCTTATGGTGGTAGGGGTGGTCCGTATGGTGGCTATGGAAGTGAGTTTGCTGGGTCTGGTGGATATGCTGGAGCAATGGGGGGACCCTACAGAGGAGATCCTTCTCTAGCATATGCAGGTCGTTATGGCGGAAGCTATGATCCTAGTGGGTATGGTGGACTTGGTGAGAGTTATGGGGCATATGGTGGTGCCGGTGCAGGTGCTGCTGCTGGCGGAGGTGTCGATTCTGAAAATATAAAGCAAAAACAAGGTTCAAAAGGTTGTGTATGTGCAGATGTCAACAAAAAAGCAAATGAAACAGATGTTAGAAAGGCAAGAAAAGCTGAACTCCAAAGAGCTGAAGCAACTGGGTTAAATCAATCAATGGAATCACCCAAAAACGATCACATCATTGGAACCGGAGGTGAACAAAACGACGTCGGTAGAAACCATGAACACGAACATGAACAACAACATCATAACTCTCAATCCCTTACCGGTGATGGTGCTAGTCCTGGGTAA |
| Protein sequence |
>MsG0680030351.01.T01 MRDHSTNRSRGFGFVTFDTEEAVDDLLSMGNKIEFAGTQVEIKKAEPKKANAPPPSSKRYNDSRSSYGSGGYGDAYDGFGGVGGGYSRSGSAYGGRGGPYGGYGSEFAGSGGYAGAMGGPYRGDPSLAYAGRYGGSYDPSGYGGLGESYGAYGGAGAGAAAGGGVDSENIKQKQGSKGCVCADVNKKANETDVRKARKAELQRAEATGLNQSMESPKNDHIIGTGGEQNDVGRNHEHEHEQQHHNSQSLTGDGASPG* >MsG0680030351.01.T03 MESPKNDHIIGTGGEQNDVGRNHEHEHEQQHHNSQSLTGDGASPGKIFIGGLARETTIAQFIKHFGKYGEITDSVIMKDRKTGQPRGFGFITYADPSVVDKVIETLISSTANKYDCFFLPCLCIGFYSFCFDYIVEIKRTIPRGAVGSKDFRTKKIFVGGIPSNVTEGRLSV* >MsG0680030351.01.T02 MRDHSTNRSRGFGFVTFDTEEAVDDLLSMGNKIEFAGTQVEIKKAEPKKANAPPPSSKRYNDSRSSYGSGGYGDAYDGFGGVGGGYSRSGSAYGGRGGPYGGYGSEFAGSGGYAGAMGGPYRGDPSLAYAGRYGGSYDPSGYGGLGESYGAYGGAGAGAAAGGGVDSENIKQKQGSKGCVCADVNKKANETDVRKARKAELQRAEATGLNQSMESPKNDHIIGTGGEQNDVGRNHEHEHEQQHHNSQSLTGDGASPG* |
