Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180002312.01.T01 | RHN76652.1 | 83.478 | 230 | 7 | 1 | 1 | 199 | 66 | 295 | 7.67E-131 | 382 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180002312.01.T01 | A0A072VCE3 | 83.478 | 230 | 7 | 1 | 1 | 199 | 141 | 370 | 2.55e-130 | 383 |
| MsG0180002312.01.T01 | A0A396JH80 | 83.478 | 230 | 7 | 1 | 1 | 199 | 66 | 295 | 3.66e-131 | 382 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180002312.01 | MsG0180002315.01 | 0.805297 | 1.428693e-49 | 7.531258e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180002312.01.T01 | MTR_1g006695 | 83.478 | 230 | 7 | 1 | 1 | 199 | 141 | 370 | 6.47e-134 | 383 |
| MsG0180002312.01.T01 | MTR_3g109650 | 59.375 | 224 | 56 | 4 | 1 | 192 | 57 | 277 | 1.72e-83 | 255 |
| MsG0180002312.01.T01 | MTR_3g109650 | 59.375 | 224 | 56 | 4 | 1 | 192 | 155 | 375 | 4.06e-82 | 254 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180002312.01.T01 | AT5G11580 | 46.696 | 227 | 83 | 5 | 1 | 192 | 2 | 225 | 1.09e-58 | 189 |
| MsG0180002312.01.T01 | AT5G11580 | 46.460 | 226 | 85 | 4 | 1 | 192 | 153 | 376 | 3.06e-57 | 189 |
| MsG0180002312.01.T01 | AT5G63860 | 29.375 | 160 | 82 | 6 | 33 | 171 | 57 | 206 | 6.29e-11 | 61.2 |
Find 49 sgRNAs with CRISPR-Local
Find 63 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACATTTGGTCTTGGTGAAAA+TGG | 0.266531 | 1:+36566787 | MsG0180002312.01.T01:CDS |
| GGCAATTCTTGAACAAGTTC+AGG | 0.304428 | 1:-36566847 | None:intergenic |
| CTTTGTTAACTCATAGAAAA+AGG | 0.333392 | 1:+36566731 | MsG0180002312.01.T01:CDS |
| ATTTCACTCCAACACCAGTA+TGG | 0.359909 | 1:+36566460 | MsG0180002312.01.T01:CDS |
| TTCTAAAACTTAGCCTCATA+AGG | 0.388438 | 1:-36567042 | None:intergenic |
| TGGGGAATGGAGAAGGGTCT+TGG | 0.396465 | 1:+36566949 | MsG0180002312.01.T01:CDS |
| CTTGTGTTGTTTCGTCGGAC+GGG | 0.396749 | 1:+36566914 | MsG0180002312.01.T01:CDS |
| AAAGAAGGTATATTCTCAAT+AGG | 0.401673 | 1:+36566432 | MsG0180002312.01.T01:CDS |
| TATCCGGATGCGCGAGTTGT+TGG | 0.412533 | 1:+36566976 | MsG0180002312.01.T01:CDS |
| GAAAGTGTGTGTTGGACATT+TGG | 0.413664 | 1:+36566772 | MsG0180002312.01.T01:CDS |
| TATTGTAAGCAATCCTTATG+AGG | 0.417836 | 1:+36567029 | MsG0180002312.01.T01:CDS |
| TCACCAACAACTCGCGCATC+CGG | 0.421376 | 1:-36566979 | None:intergenic |
| GGACGGGGATGTTTGGTCGT+GGG | 0.423753 | 1:+36566930 | MsG0180002312.01.T01:CDS |
| TTTCGTCGGACGGGGATGTT+TGG | 0.425452 | 1:+36566923 | MsG0180002312.01.T01:CDS |
| TCACTATCTATTGACAATCT+TGG | 0.426654 | 1:+36566372 | MsG0180002312.01.T01:CDS |
| AATTTGATCAAGATTCTCAT+TGG | 0.433626 | 1:+36566671 | MsG0180002312.01.T01:intron |
| GGATGTTTGGTCGTGGGGAA+TGG | 0.440538 | 1:+36566936 | MsG0180002312.01.T01:CDS |
| GAAACAAGATTTACAAATTG+TGG | 0.452095 | 1:-36566868 | None:intergenic |
| GAAGGGTCTTGGTTTGTATC+CGG | 0.461196 | 1:+36566960 | MsG0180002312.01.T01:CDS |
| TTGACAATCTTGGTGCCCTA+TGG | 0.467260 | 1:+36566382 | MsG0180002312.01.T01:CDS |
| GTGTGTTGGACATTTGGTCT+TGG | 0.480962 | 1:+36566778 | MsG0180002312.01.T01:CDS |
| TTGTGTTGTTTCGTCGGACG+GGG | 0.482207 | 1:+36566915 | MsG0180002312.01.T01:CDS |
| TCATACTTGTGTTGTTTCGT+CGG | 0.493278 | 1:+36566909 | MsG0180002312.01.T01:CDS |
| ACTTGTGTTGTTTCGTCGGA+CGG | 0.501899 | 1:+36566913 | MsG0180002312.01.T01:CDS |
| ATCTTGGTGCCCTATGGATG+TGG | 0.506487 | 1:+36566388 | MsG0180002312.01.T01:CDS |
| TTGGTCGTGGGGAATGGAGA+AGG | 0.511138 | 1:+36566942 | MsG0180002312.01.T01:CDS |
| ATTATAGCACTAGTTAGTGT+TGG | 0.512070 | 1:+36566531 | MsG0180002312.01.T01:CDS |
| ATACCTTCTTTGCTTTGATG+TGG | 0.514646 | 1:-36566420 | None:intergenic |
| CGGACGGGGATGTTTGGTCG+TGG | 0.518856 | 1:+36566929 | MsG0180002312.01.T01:CDS |
| GACAGTTTCCCCACATCCAT+AGG | 0.520889 | 1:-36566398 | None:intergenic |
| TGGAAATCCCATACTGGTGT+TGG | 0.534551 | 1:-36566468 | None:intergenic |
| TTTCACTCCAACACCAGTAT+GGG | 0.536712 | 1:+36566461 | MsG0180002312.01.T01:CDS |
| TGTCCACATCAAAGCAAAGA+AGG | 0.542595 | 1:+36566417 | MsG0180002312.01.T01:CDS |
| GACGGGGATGTTTGGTCGTG+GGG | 0.558285 | 1:+36566931 | MsG0180002312.01.T01:CDS |
| CTTGGTGAAAATGGACAACT+TGG | 0.566883 | 1:+36566796 | MsG0180002312.01.T01:CDS |
| AATCTTGTTTCTGTTAACTG+TGG | 0.570236 | 1:+36566880 | MsG0180002312.01.T01:CDS |
| ATACTGGTGTTGGAGTGAAA+TGG | 0.571831 | 1:-36566458 | None:intergenic |
| TGACCATGGAAATCCCATAC+TGG | 0.572116 | 1:-36566474 | None:intergenic |
| TGGTCGTGGGGAATGGAGAA+GGG | 0.577448 | 1:+36566943 | MsG0180002312.01.T01:CDS |
| TAAGGATTGCTTACAATAAG+CGG | 0.586907 | 1:-36567024 | None:intergenic |
| GAAAATGGACAACTTGGTCA+TGG | 0.608046 | 1:+36566802 | MsG0180002312.01.T01:CDS |
| TCTTGGTGCCCTATGGATGT+GGG | 0.615493 | 1:+36566389 | MsG0180002312.01.T01:CDS |
| ATACATTAGAAAGTGTGTGT+TGG | 0.618605 | 1:+36566764 | MsG0180002312.01.T01:CDS |
| ACAGTTTCCCCACATCCATA+GGG | 0.620374 | 1:-36566397 | None:intergenic |
| ACACCAGTATGGGATTTCCA+TGG | 0.640967 | 1:+36566471 | MsG0180002312.01.T01:CDS |
| ACAGTAGTAAAAGTTGCATG+TGG | 0.647702 | 1:+36566498 | MsG0180002312.01.T01:CDS |
| AGTGTTGGAGAATCATACAA+TGG | 0.686513 | 1:+36566546 | MsG0180002312.01.T01:CDS |
| CTTGGTGCCCTATGGATGTG+GGG | 0.689350 | 1:+36566390 | MsG0180002312.01.T01:CDS |
| TCAATTTATGAAATATCATG+TGG | 0.707994 | 1:+36566694 | MsG0180002312.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TCAATTTATGAAATATCATG+TGG | + | Chr1:36566694-36566713 | MsG0180002312.01.T01:CDS | 20.0% |
| !!! | ATCAAATTTTTTACCATTTC+TGG | - | Chr1:36566659-36566678 | None:intergenic | 20.0% |
| ! | AATTTGATCAAGATTCTCAT+TGG | + | Chr1:36566671-36566690 | MsG0180002312.01.T01:intron | 25.0% |
| ! | CTTTGTTAACTCATAGAAAA+AGG | + | Chr1:36566731-36566750 | MsG0180002312.01.T01:CDS | 25.0% |
| ! | GAAACAAGATTTACAAATTG+TGG | - | Chr1:36566871-36566890 | None:intergenic | 25.0% |
| !! | AAAGAAGGTATATTCTCAAT+AGG | + | Chr1:36566432-36566451 | MsG0180002312.01.T01:CDS | 25.0% |
| !!! | TTTTTTACCATTTCTGGATT+TGG | - | Chr1:36566653-36566672 | None:intergenic | 25.0% |
| AACAACAATCATGGTCAATT+AGG | + | Chr1:36566605-36566624 | MsG0180002312.01.T01:intron | 30.0% | |
| ATTATAGCACTAGTTAGTGT+TGG | + | Chr1:36566531-36566550 | MsG0180002312.01.T01:CDS | 30.0% | |
| TAAGGATTGCTTACAATAAG+CGG | - | Chr1:36567027-36567046 | None:intergenic | 30.0% | |
| TATTGTAAGCAATCCTTATG+AGG | + | Chr1:36567029-36567048 | MsG0180002312.01.T01:CDS | 30.0% | |
| TCACTATCTATTGACAATCT+TGG | + | Chr1:36566372-36566391 | MsG0180002312.01.T01:CDS | 30.0% | |
| ! | ATACATTAGAAAGTGTGTGT+TGG | + | Chr1:36566764-36566783 | MsG0180002312.01.T01:CDS | 30.0% |
| ! | ATGATCTCATTTGTTACTCT+TGG | + | Chr1:36566577-36566596 | MsG0180002312.01.T01:intron | 30.0% |
| ! | TGATCTCATTTGTTACTCTT+GGG | + | Chr1:36566578-36566597 | MsG0180002312.01.T01:intron | 30.0% |
| !! | AATCTTGTTTCTGTTAACTG+TGG | + | Chr1:36566880-36566899 | MsG0180002312.01.T01:CDS | 30.0% |
| AATCATGGTCAATTAGGCTT+AGG | + | Chr1:36566611-36566630 | MsG0180002312.01.T01:intron | 35.0% | |
| ACAGTAGTAAAAGTTGCATG+TGG | + | Chr1:36566498-36566517 | MsG0180002312.01.T01:CDS | 35.0% | |
| ATACCTTCTTTGCTTTGATG+TGG | - | Chr1:36566423-36566442 | None:intergenic | 35.0% | |
| ATCATGGTCAATTAGGCTTA+GGG | + | Chr1:36566612-36566631 | MsG0180002312.01.T01:intron | 35.0% | |
| ! | AGTGTTGGAGAATCATACAA+TGG | + | Chr1:36566546-36566565 | MsG0180002312.01.T01:CDS | 35.0% |
| ! | ATCTCATTTGTTACTCTTGG+GGG | + | Chr1:36566580-36566599 | MsG0180002312.01.T01:intron | 35.0% |
| ! | GATCTCATTTGTTACTCTTG+GGG | + | Chr1:36566579-36566598 | MsG0180002312.01.T01:intron | 35.0% |
| !! | ACATTTGGTCTTGGTGAAAA+TGG | + | Chr1:36566787-36566806 | MsG0180002312.01.T01:CDS | 35.0% |
| !! | TCATACTTGTGTTGTTTCGT+CGG | + | Chr1:36566909-36566928 | MsG0180002312.01.T01:CDS | 35.0% |
| ATTTCACTCCAACACCAGTA+TGG | + | Chr1:36566460-36566479 | MsG0180002312.01.T01:CDS | 40.0% | |
| CGATAGACCAAATCCAGAAA+TGG | + | Chr1:36566643-36566662 | MsG0180002312.01.T01:intron | 40.0% | |
| CTTGGTGAAAATGGACAACT+TGG | + | Chr1:36566796-36566815 | MsG0180002312.01.T01:CDS | 40.0% | |
| GAAAATGGACAACTTGGTCA+TGG | + | Chr1:36566802-36566821 | MsG0180002312.01.T01:CDS | 40.0% | |
| GGCAATTCTTGAACAAGTTC+AGG | - | Chr1:36566850-36566869 | None:intergenic | 40.0% | |
| TCATGGTCAATTAGGCTTAG+GGG | + | Chr1:36566613-36566632 | MsG0180002312.01.T01:intron | 40.0% | |
| TGTCCACATCAAAGCAAAGA+AGG | + | Chr1:36566417-36566436 | MsG0180002312.01.T01:CDS | 40.0% | |
| TTTCACTCCAACACCAGTAT+GGG | + | Chr1:36566461-36566480 | MsG0180002312.01.T01:CDS | 40.0% | |
| ! | ATACTGGTGTTGGAGTGAAA+TGG | - | Chr1:36566461-36566480 | None:intergenic | 40.0% |
| ! | CTTTTACTACTGTGTGACCA+TGG | - | Chr1:36566491-36566510 | None:intergenic | 40.0% |
| ! | GAAAGTGTGTGTTGGACATT+TGG | + | Chr1:36566772-36566791 | MsG0180002312.01.T01:CDS | 40.0% |
| ! | TCTCATTTGTTACTCTTGGG+GGG | + | Chr1:36566581-36566600 | MsG0180002312.01.T01:intron | 40.0% |
| ACACCAGTATGGGATTTCCA+TGG | + | Chr1:36566471-36566490 | MsG0180002312.01.T01:CDS | 45.0% | |
| ACAGTTTCCCCACATCCATA+GGG | - | Chr1:36566400-36566419 | None:intergenic | 45.0% | |
| GTGTGTTGGACATTTGGTCT+TGG | + | Chr1:36566778-36566797 | MsG0180002312.01.T01:CDS | 45.0% | |
| TCAATTAGGCTTAGGGGACA+CGG | + | Chr1:36566619-36566638 | MsG0180002312.01.T01:intron | 45.0% | |
| TGACCATGGAAATCCCATAC+TGG | - | Chr1:36566477-36566496 | None:intergenic | 45.0% | |
| TGGAAATCCCATACTGGTGT+TGG | - | Chr1:36566471-36566490 | None:intergenic | 45.0% | |
| TTGGGGGGTAACAACAATCA+TGG | + | Chr1:36566596-36566615 | MsG0180002312.01.T01:intron | 45.0% | |
| !! | ACTTGTGTTGTTTCGTCGGA+CGG | + | Chr1:36566913-36566932 | MsG0180002312.01.T01:CDS | 45.0% |
| !! | GAAGGGTCTTGGTTTGTATC+CGG | + | Chr1:36566960-36566979 | MsG0180002312.01.T01:CDS | 45.0% |
| !! | TTGACAATCTTGGTGCCCTA+TGG | + | Chr1:36566382-36566401 | MsG0180002312.01.T01:CDS | 45.0% |
| GACAGTTTCCCCACATCCAT+AGG | - | Chr1:36566401-36566420 | None:intergenic | 50.0% | |
| !! | ATCTTGGTGCCCTATGGATG+TGG | + | Chr1:36566388-36566407 | MsG0180002312.01.T01:CDS | 50.0% |
| !! | CTTGTGTTGTTTCGTCGGAC+GGG | + | Chr1:36566914-36566933 | MsG0180002312.01.T01:CDS | 50.0% |
| !! | TCTTGGTGCCCTATGGATGT+GGG | + | Chr1:36566389-36566408 | MsG0180002312.01.T01:CDS | 50.0% |
| !! | TTGTGTTGTTTCGTCGGACG+GGG | + | Chr1:36566915-36566934 | MsG0180002312.01.T01:CDS | 50.0% |
| GGATGTTTGGTCGTGGGGAA+TGG | + | Chr1:36566936-36566955 | MsG0180002312.01.T01:CDS | 55.0% | |
| TATCCGGATGCGCGAGTTGT+TGG | + | Chr1:36566976-36566995 | MsG0180002312.01.T01:CDS | 55.0% | |
| TCACCAACAACTCGCGCATC+CGG | - | Chr1:36566982-36567001 | None:intergenic | 55.0% | |
| TGGGGAATGGAGAAGGGTCT+TGG | + | Chr1:36566949-36566968 | MsG0180002312.01.T01:CDS | 55.0% | |
| TGGTCGTGGGGAATGGAGAA+GGG | + | Chr1:36566943-36566962 | MsG0180002312.01.T01:CDS | 55.0% | |
| TTGGTCGTGGGGAATGGAGA+AGG | + | Chr1:36566942-36566961 | MsG0180002312.01.T01:CDS | 55.0% | |
| TTTCGTCGGACGGGGATGTT+TGG | + | Chr1:36566923-36566942 | MsG0180002312.01.T01:CDS | 55.0% | |
| !! | CTTGGTGCCCTATGGATGTG+GGG | + | Chr1:36566390-36566409 | MsG0180002312.01.T01:CDS | 55.0% |
| GACGGGGATGTTTGGTCGTG+GGG | + | Chr1:36566931-36566950 | MsG0180002312.01.T01:CDS | 60.0% | |
| GGACGGGGATGTTTGGTCGT+GGG | + | Chr1:36566930-36566949 | MsG0180002312.01.T01:CDS | 60.0% | |
| CGGACGGGGATGTTTGGTCG+TGG | + | Chr1:36566929-36566948 | MsG0180002312.01.T01:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr1 | gene | 36566369 | 36567062 | 36566369 | ID=MsG0180002312.01;Name=MsG0180002312.01 |
| Chr1 | mRNA | 36566369 | 36567062 | 36566369 | ID=MsG0180002312.01.T01;Parent=MsG0180002312.01;Name=MsG0180002312.01.T01;_AED=0.35;_eAED=0.35;_QI=0|0|0|1|1|1|2|0|199 |
| Chr1 | exon | 36566369 | 36566588 | 36566369 | ID=MsG0180002312.01.T01:exon:113;Parent=MsG0180002312.01.T01 |
| Chr1 | exon | 36566683 | 36567062 | 36566683 | ID=MsG0180002312.01.T01:exon:112;Parent=MsG0180002312.01.T01 |
| Chr1 | CDS | 36566369 | 36566588 | 36566369 | ID=MsG0180002312.01.T01:cds;Parent=MsG0180002312.01.T01 |
| Chr1 | CDS | 36566683 | 36567062 | 36566683 | ID=MsG0180002312.01.T01:cds;Parent=MsG0180002312.01.T01 |
| Gene Sequence |
| Protein sequence |
