Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180002982.01.T01 | KEH41709.1 | 74.468 | 188 | 2 | 1 | 1 | 188 | 149 | 290 | 6.15E-83 | 257 |
| MsG0180002982.01.T02 | KEH41709.1 | 74.468 | 188 | 2 | 1 | 1 | 188 | 149 | 290 | 6.15E-83 | 257 |
| MsG0180002982.01.T03 | GAU37525.1 | 92.754 | 138 | 8 | 2 | 6 | 142 | 4 | 140 | 5.84E-85 | 260 |
| MsG0180002982.01.T04 | GAU37525.1 | 92.754 | 138 | 8 | 2 | 6 | 142 | 4 | 140 | 5.84E-85 | 260 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180002982.01.T01 | Q8S9M1 | 49.468 | 188 | 48 | 2 | 1 | 188 | 159 | 299 | 2.11E-51 | 170 |
| MsG0180002982.01.T02 | Q8S9M1 | 49.468 | 188 | 48 | 2 | 1 | 188 | 159 | 299 | 2.11E-51 | 170 |
| MsG0180002982.01.T03 | Q8S9M1 | 70.4 | 125 | 31 | 3 | 23 | 142 | 24 | 147 | 1.45E-53 | 173 |
| MsG0180002982.01.T04 | Q8S9M1 | 70.4 | 125 | 31 | 3 | 23 | 142 | 24 | 147 | 1.45E-53 | 173 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180002982.01.T01 | A0A072VI54 | 74.468 | 188 | 2 | 1 | 1 | 188 | 149 | 290 | 2.94e-83 | 257 |
| MsG0180002982.01.T02 | A0A072VI54 | 74.468 | 188 | 2 | 1 | 1 | 188 | 149 | 290 | 2.94e-83 | 257 |
| MsG0180002982.01.T03 | A0A2Z6NNT4 | 92.754 | 138 | 8 | 2 | 6 | 142 | 4 | 140 | 2.79e-85 | 260 |
| MsG0180002982.01.T04 | A0A2Z6NNT4 | 92.754 | 138 | 8 | 2 | 6 | 142 | 4 | 140 | 2.79e-85 | 260 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080047831.01 | MsG0180002982.01 | -0.809905 | 1.501646e-50 | 8.926316e-48 |
| MsG0080047910.01 | MsG0180002982.01 | 0.847880 | 8.740070e-60 | 1.575496e-56 |
| MsG0080047945.01 | MsG0180002982.01 | 0.805722 | 1.163993e-49 | 6.203614e-47 |
| MsG0080047955.01 | MsG0180002982.01 | 0.801654 | 8.130403e-49 | 3.903920e-46 |
| MsG0080047956.01 | MsG0180002982.01 | 0.817808 | 2.720752e-52 | 2.001699e-49 |
| MsG0080047989.01 | MsG0180002982.01 | 0.829558 | 4.814073e-55 | 4.944028e-52 |
| MsG0080048059.01 | MsG0180002982.01 | 0.885012 | 1.173418e-71 | 7.921685e-68 |
| MsG0080048068.01 | MsG0180002982.01 | 0.807614 | 4.638954e-50 | 2.595524e-47 |
| MsG0080048202.01 | MsG0180002982.01 | -0.813888 | 2.038091e-51 | 1.347308e-48 |
| MsG0080048256.01 | MsG0180002982.01 | -0.869806 | 2.348944e-66 | 8.930926e-63 |
PPI
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180002982.01.T01 | MTR_1g054070 | 74.468 | 188 | 2 | 1 | 1 | 188 | 149 | 290 | 7.44e-87 | 257 |
| MsG0180002982.01.T01 | MTR_1g054070 | 74.468 | 188 | 2 | 1 | 1 | 188 | 156 | 297 | 9.21e-87 | 257 |
| MsG0180002982.01.T02 | MTR_1g054070 | 74.468 | 188 | 2 | 1 | 1 | 188 | 149 | 290 | 7.44e-87 | 257 |
| MsG0180002982.01.T02 | MTR_1g054070 | 74.468 | 188 | 2 | 1 | 1 | 188 | 156 | 297 | 9.21e-87 | 257 |
| MsG0180002982.01.T03 | MTR_1g054070 | 96.970 | 132 | 4 | 0 | 11 | 142 | 13 | 144 | 8.34e-87 | 255 |
| MsG0180002982.01.T03 | MTR_1g054070 | 91.667 | 132 | 4 | 1 | 11 | 142 | 13 | 137 | 1.67e-78 | 234 |
| MsG0180002982.01.T04 | MTR_1g054070 | 96.970 | 132 | 4 | 0 | 11 | 142 | 13 | 144 | 8.34e-87 | 255 |
| MsG0180002982.01.T04 | MTR_1g054070 | 91.667 | 132 | 4 | 1 | 11 | 142 | 13 | 137 | 1.67e-78 | 234 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180002982.01.T01 | AT2G42130 | 49.468 | 188 | 48 | 2 | 1 | 188 | 64 | 204 | 3.45e-53 | 169 |
| MsG0180002982.01.T01 | AT2G42130 | 49.468 | 188 | 48 | 2 | 1 | 188 | 131 | 271 | 1.20e-52 | 169 |
| MsG0180002982.01.T01 | AT2G42130 | 49.468 | 188 | 48 | 2 | 1 | 188 | 159 | 299 | 2.15e-52 | 170 |
| MsG0180002982.01.T01 | AT3G58010 | 48.851 | 174 | 43 | 1 | 1 | 174 | 164 | 291 | 3.88e-51 | 167 |
| MsG0180002982.01.T01 | AT3G58010 | 48.851 | 174 | 43 | 1 | 1 | 174 | 164 | 291 | 3.88e-51 | 167 |
| MsG0180002982.01.T01 | AT2G42130 | 49.682 | 157 | 33 | 1 | 1 | 157 | 159 | 269 | 1.90e-42 | 143 |
| MsG0180002982.01.T01 | AT2G42130 | 67.708 | 96 | 31 | 0 | 1 | 96 | 159 | 254 | 6.85e-40 | 137 |
| MsG0180002982.01.T02 | AT2G42130 | 49.468 | 188 | 48 | 2 | 1 | 188 | 64 | 204 | 3.45e-53 | 169 |
| MsG0180002982.01.T02 | AT2G42130 | 49.468 | 188 | 48 | 2 | 1 | 188 | 131 | 271 | 1.20e-52 | 169 |
| MsG0180002982.01.T02 | AT2G42130 | 49.468 | 188 | 48 | 2 | 1 | 188 | 159 | 299 | 2.15e-52 | 170 |
Find 0 sgRNAs with CRISPR-Local
Find 277 sgRNAs with CRISPR-GE
CRISPR-Local
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AACAATCCGAAGAAAAACTT+CGG | + | Chr1:54298447-54298466 | None:intergenic | 30.0% | |
| AAGCAAATAATGAGAAAAGG+AGG | - | Chr1:54297168-54297187 | MsG0180002982.01.T01:intron | 30.0% | |
| AATAACAGTTGCAAGTAATG+AGG | - | Chr1:54297780-54297799 | MsG0180002982.01.T01:intron | 30.0% | |
| ACATTCAACTCACTTTCAAT+CGG | - | Chr1:54297632-54297651 | MsG0180002982.01.T01:intron | 30.0% | |
| AGCATGTATAAGAGAATTGA+GGG | + | Chr1:54300548-54300567 | None:intergenic | 30.0% | |
| AGGCAAAGAATCAGTTATAA+AGG | + | Chr1:54297463-54297482 | None:intergenic | 30.0% | |
| ATGTGAGAATGATAGAATTG+AGG | + | Chr1:54300339-54300358 | None:intergenic | 30.0% | |
| CAAAATGGATGTCTTTATCA+AGG | - | Chr1:54298572-54298591 | MsG0180002982.01.T01:CDS | 30.0% | |
| CAACAACTTCAAAATATGGA+AGG | - | Chr1:54300228-54300247 | MsG0180002982.01.T01:intron | 30.0% | |
| CAATTGTAGAAGCAAAATGA+CGG | + | Chr1:54297547-54297566 | None:intergenic | 30.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr1 | gene | 54296749 | 54301072 | 54296749 | ID=MsG0180002982.01;Name=MsG0180002982.01 |
| Chr1 | mRNA | 54296749 | 54301070 | 54296749 | ID=MsG0180002982.01.T01;Parent=MsG0180002982.01;Name=MsG0180002982.01.T01;_AED=0.38;_eAED=0.42;_QI=535|0.85|0.87|1|0.28|0.37|8|240|188 |
| Chr1 | five_prime_UTR | 54301015 | 54301070 | 54301015 | ID=MsG0180002982.01.T01:five_prime_utr;Parent=MsG0180002982.01.T01 |
| Chr1 | five_prime_UTR | 54300745 | 54300984 | 54300745 | ID=MsG0180002982.01.T01:five_prime_utr;Parent=MsG0180002982.01.T01 |
| Chr1 | five_prime_UTR | 54299574 | 54299695 | 54299574 | ID=MsG0180002982.01.T01:five_prime_utr;Parent=MsG0180002982.01.T01 |
| Chr1 | five_prime_UTR | 54299412 | 54299495 | 54299412 | ID=MsG0180002982.01.T01:five_prime_utr;Parent=MsG0180002982.01.T01 |
| Chr1 | five_prime_UTR | 54299246 | 54299278 | 54299246 | ID=MsG0180002982.01.T01:five_prime_utr;Parent=MsG0180002982.01.T01 |
| Chr1 | CDS | 54299183 | 54299245 | 54299183 | ID=MsG0180002982.01.T01:cds;Parent=MsG0180002982.01.T01 |
| Chr1 | CDS | 54298485 | 54298839 | 54298485 | ID=MsG0180002982.01.T01:cds;Parent=MsG0180002982.01.T01 |
| Chr1 | CDS | 54297971 | 54298023 | 54297971 | ID=MsG0180002982.01.T01:cds;Parent=MsG0180002982.01.T01 |
| Chr1 | CDS | 54296989 | 54297084 | 54296989 | ID=MsG0180002982.01.T01:cds;Parent=MsG0180002982.01.T01 |
| Chr1 | three_prime_UTR | 54296749 | 54296988 | 54296749 | ID=MsG0180002982.01.T01:three_prime_utr;Parent=MsG0180002982.01.T01 |
| Chr1 | mRNA | 54296749 | 54301072 | 54296749 | ID=MsG0180002982.01.T02;Parent=MsG0180002982.01;Name=MsG0180002982.01.T02;_AED=0.38;_eAED=0.41;_QI=567|0.83|0.85|1|0.33|0.57|7|240|188 |
| Chr1 | five_prime_UTR | 54300745 | 54301072 | 54300745 | ID=MsG0180002982.01.T02:five_prime_utr;Parent=MsG0180002982.01.T02 |
| Chr1 | five_prime_UTR | 54299574 | 54299695 | 54299574 | ID=MsG0180002982.01.T02:five_prime_utr;Parent=MsG0180002982.01.T02 |
| Chr1 | five_prime_UTR | 54299412 | 54299495 | 54299412 | ID=MsG0180002982.01.T02:five_prime_utr;Parent=MsG0180002982.01.T02 |
| Chr1 | five_prime_UTR | 54299246 | 54299278 | 54299246 | ID=MsG0180002982.01.T02:five_prime_utr;Parent=MsG0180002982.01.T02 |
| Chr1 | CDS | 54299183 | 54299245 | 54299183 | ID=MsG0180002982.01.T02:cds;Parent=MsG0180002982.01.T02 |
| Chr1 | CDS | 54298485 | 54298839 | 54298485 | ID=MsG0180002982.01.T02:cds;Parent=MsG0180002982.01.T02 |
| Chr1 | CDS | 54297971 | 54298023 | 54297971 | ID=MsG0180002982.01.T02:cds;Parent=MsG0180002982.01.T02 |
| Chr1 | CDS | 54296989 | 54297084 | 54296989 | ID=MsG0180002982.01.T02:cds;Parent=MsG0180002982.01.T02 |
| Chr1 | three_prime_UTR | 54296749 | 54296988 | 54296749 | ID=MsG0180002982.01.T02:three_prime_utr;Parent=MsG0180002982.01.T02 |
| Chr1 | mRNA | 54299408 | 54301070 | 54299408 | ID=MsG0180002982.01.T03;Parent=MsG0180002982.01;Name=MsG0180002982.01.T03;_AED=0.46;_eAED=0.46;_QI=77|1|0.75|1|0.66|0.5|4|0|142 |
| Chr1 | five_prime_UTR | 54301015 | 54301070 | 54301015 | ID=MsG0180002982.01.T03:five_prime_utr;Parent=MsG0180002982.01.T03 |
| Chr1 | five_prime_UTR | 54300964 | 54300984 | 54300964 | ID=MsG0180002982.01.T03:five_prime_utr;Parent=MsG0180002982.01.T03 |
| Chr1 | CDS | 54300745 | 54300963 | 54300745 | ID=MsG0180002982.01.T03:cds;Parent=MsG0180002982.01.T03 |
| Chr1 | CDS | 54299574 | 54299695 | 54299574 | ID=MsG0180002982.01.T03:cds;Parent=MsG0180002982.01.T03 |
| Chr1 | CDS | 54299408 | 54299495 | 54299408 | ID=MsG0180002982.01.T03:cds;Parent=MsG0180002982.01.T03 |
| Chr1 | mRNA | 54299408 | 54301072 | 54299408 | ID=MsG0180002982.01.T04;Parent=MsG0180002982.01;Name=MsG0180002982.01.T04;_AED=0.45;_eAED=0.45;_QI=109|1|0.66|1|1|1|3|0|142 |
| Chr1 | five_prime_UTR | 54300964 | 54301072 | 54300964 | ID=MsG0180002982.01.T04:five_prime_utr;Parent=MsG0180002982.01.T04 |
| Chr1 | CDS | 54300745 | 54300963 | 54300745 | ID=MsG0180002982.01.T04:cds;Parent=MsG0180002982.01.T04 |
| Chr1 | CDS | 54299574 | 54299695 | 54299574 | ID=MsG0180002982.01.T04:cds;Parent=MsG0180002982.01.T04 |
| Chr1 | CDS | 54299408 | 54299495 | 54299408 | ID=MsG0180002982.01.T04:cds;Parent=MsG0180002982.01.T04 |
| Gene Sequence |
>MsG0180002982.01.T01 ATGGATGTCTTTATCAAGGATGGAAATGCAAAGATTACCGCAAACACATTATTTTTAAACTCGATAGAAAGCAGAATTATTCTCTCAACCAAGTTAACTGTGGAGGGGCCACTTAGAATGAAGGAGGAGTATGTGGAAGGGATTATTGTGTCACCAACAGTCTTGGAAGACAGAGTACCAGATCAGCTTAAAGGTGCACTTGGGCAGGCAGTTAATGTTTTACAACAGCTTCCCGTACCTTTACGTGATGCTCTCGCCGATGGGCTAAAAGTTCCACTAAGTAAGCTACCACTGATTTCTATATTCACCTCTGAAAATGTTCTCTGGCTTAACTGTTCTGGAAGAAATATAAAATCTGTTAAGTATTGGTTTCACTATGTGCTGCTAATATTAGGGCCTGTTTATTCTGTTGTTTCGTGTGGAAGCTTTCAAAGACTCTTCATGATCTCTTATCTCGATGAGGAGATACTTATAATAAGGAACACAGCTGGGATACCAGAAGTTTTAACGAGACTCGATGCATCGCCGTCATCCCTGGGAGAATCAAGTCCAGATTATGAAAGCTAG >MsG0180002982.01.T03 ATGGCTGTTTCTGTATCTATTTCTGCTTCTCATCCTTGTATTTCAACAATCACAAATTGTTCAATCCCTTCATCTTCTTCATGGAAAGTTTCAACTTCACGCATCACGTGGCAGAAGCGTGGAATTTGCAGAGCTATGGTTCAAGTTCAAACTGGTGCACCTGCTGCTTATGCTAAGGAAATGGAACGCCTTTCTGCTAAAGAATCGCTTCTTCTTGCTTTTAAGGATGCAGGTGGTTTTGAGAGCTTAGTCTCTGGGAAGACAACTGAATATCAAAGAATTGATGTGAATGAAAGGATAACGGGTCTTGAGCGGCTCAATCCAACACCTCGGCCCACGACGTCACCCTTTCTAGAAGGTAGATGGAACTTTGAGTGGTTTGGTCCCGGCTCTCCAGGGTTGTTTGCGGCTAGGATAATATTTGAGTGA >MsG0180002982.01.T02 ATGGATGTCTTTATCAAGGATGGAAATGCAAAGATTACCGCAAACACATTATTTTTAAACTCGATAGAAAGCAGAATTATTCTCTCAACCAAGTTAACTGTGGAGGGGCCACTTAGAATGAAGGAGGAGTATGTGGAAGGGATTATTGTGTCACCAACAGTCTTGGAAGACAGAGTACCAGATCAGCTTAAAGGTGCACTTGGGCAGGCAGTTAATGTTTTACAACAGCTTCCCGTACCTTTACGTGATGCTCTCGCCGATGGGCTAAAAGTTCCACTAAGTAAGCTACCACTGATTTCTATATTCACCTCTGAAAATGTTCTCTGGCTTAACTGTTCTGGAAGAAATATAAAATCTGTTAAGTATTGGTTTCACTATGTGCTGCTAATATTAGGGCCTGTTTATTCTGTTGTTTCGTGTGGAAGCTTTCAAAGACTCTTCATGATCTCTTATCTCGATGAGGAGATACTTATAATAAGGAACACAGCTGGGATACCAGAAGTTTTAACGAGACTCGATGCATCGCCGTCATCCCTGGGAGAATCAAGTCCAGATTATGAAAGCTAG >MsG0180002982.01.T04 ATGGCTGTTTCTGTATCTATTTCTGCTTCTCATCCTTGTATTTCAACAATCACAAATTGTTCAATCCCTTCATCTTCTTCATGGAAAGTTTCAACTTCACGCATCACGTGGCAGAAGCGTGGAATTTGCAGAGCTATGGTTCAAGTTCAAACTGGTGCACCTGCTGCTTATGCTAAGGAAATGGAACGCCTTTCTGCTAAAGAATCGCTTCTTCTTGCTTTTAAGGATGCAGGTGGTTTTGAGAGCTTAGTCTCTGGGAAGACAACTGAATATCAAAGAATTGATGTGAATGAAAGGATAACGGGTCTTGAGCGGCTCAATCCAACACCTCGGCCCACGACGTCACCCTTTCTAGAAGGTAGATGGAACTTTGAGTGGTTTGGTCCCGGCTCTCCAGGGTTGTTTGCGGCTAGGATAATATTTGAGTGA |
| Protein sequence |
>MsG0180002982.01.T01 MDVFIKDGNAKITANTLFLNSIESRIILSTKLTVEGPLRMKEEYVEGIIVSPTVLEDRVPDQLKGALGQAVNVLQQLPVPLRDALADGLKVPLSKLPLISIFTSENVLWLNCSGRNIKSVKYWFHYVLLILGPVYSVVSCGSFQRLFMISYLDEEILIIRNTAGIPEVLTRLDASPSSLGESSPDYES* >MsG0180002982.01.T03 MAVSVSISASHPCISTITNCSIPSSSSWKVSTSRITWQKRGICRAMVQVQTGAPAAYAKEMERLSAKESLLLAFKDAGGFESLVSGKTTEYQRIDVNERITGLERLNPTPRPTTSPFLEGRWNFEWFGPGSPGLFAARIIFE* >MsG0180002982.01.T02 MDVFIKDGNAKITANTLFLNSIESRIILSTKLTVEGPLRMKEEYVEGIIVSPTVLEDRVPDQLKGALGQAVNVLQQLPVPLRDALADGLKVPLSKLPLISIFTSENVLWLNCSGRNIKSVKYWFHYVLLILGPVYSVVSCGSFQRLFMISYLDEEILIIRNTAGIPEVLTRLDASPSSLGESSPDYES* >MsG0180002982.01.T04 MAVSVSISASHPCISTITNCSIPSSSSWKVSTSRITWQKRGICRAMVQVQTGAPAAYAKEMERLSAKESLLLAFKDAGGFESLVSGKTTEYQRIDVNERITGLERLNPTPRPTTSPFLEGRWNFEWFGPGSPGLFAARIIFE* |
