AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0180003095.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180003095.01.T01 MTR_1g054675 95.055 182 8 1 1 182 1 181 2.47e-84 250
MsG0180003095.01.T01 MTR_3g083550 59.574 235 75 5 1 218 1 232 6.48e-63 197
MsG0180003095.01.T01 MTR_5g079870 64.045 89 32 0 45 133 48 136 7.88e-37 130
MsG0180003095.01.T01 MTR_4g118630 64.835 91 32 0 42 132 46 136 4.11e-35 127
MsG0180003095.01.T01 MTR_5g079870 38.723 235 86 6 45 221 48 282 1.47e-29 112
MsG0180003095.01.T01 MTR_5g070450 42.857 77 44 0 45 121 17 93 1.22e-12 66.2
MsG0180003095.01.T01 MTR_4g094235 41.558 77 45 0 45 121 17 93 1.31e-12 66.2
MsG0180003095.01.T01 MTR_4g094235 41.558 77 45 0 45 121 17 93 1.32e-12 66.2
MsG0180003095.01.T01 MTR_8g031560 44.706 85 44 1 26 107 178 262 2.17e-12 65.5
MsG0180003095.01.T01 MTR_5g044970 38.947 95 53 1 44 138 10 99 8.52e-12 62.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180003095.01.T01 AT3G13570 60.088 228 74 6 1 220 1 219 1.35e-66 207
MsG0180003095.01.T01 AT1G55310 63.393 224 64 6 1 216 1 214 1.22e-64 200
MsG0180003095.01.T01 AT1G55310 63.393 224 64 6 1 216 1 214 1.22e-64 200
MsG0180003095.01.T01 AT1G55310 67.000 200 50 5 1 192 1 192 1.39e-63 200
MsG0180003095.01.T01 AT1G55310 63.208 212 50 6 1 192 1 204 4.01e-60 191
MsG0180003095.01.T01 AT5G18810 57.692 104 42 1 43 144 46 149 1.63e-34 124
MsG0180003095.01.T01 AT5G18810 57.692 104 42 1 43 144 46 149 1.67e-34 124
MsG0180003095.01.T01 AT3G55460 60.215 93 37 0 46 138 49 141 5.08e-34 123
MsG0180003095.01.T01 AT1G55310 66.055 109 28 3 93 192 1 109 7.15e-24 95.5
MsG0180003095.01.T01 AT1G55310 66.055 109 28 3 93 192 1 109 7.15e-24 95.5
MsG0180003095.01.T01 AT1G55310 59.259 108 36 2 93 192 1 108 2.19e-18 80.9
MsG0180003095.01.T01 AT5G64200 44.156 77 43 0 45 121 17 93 1.30e-13 69.3
MsG0180003095.01.T01 AT5G64200 44.156 77 43 0 45 121 17 93 1.30e-13 69.3
MsG0180003095.01.T01 AT3G08000 42.857 77 44 0 46 122 43 119 3.70e-12 62.4
MsG0180003095.01.T01 AT3G10400 35.000 100 62 1 44 140 57 156 1.55e-11 62.8
MsG0180003095.01.T01 AT1G13690 37.895 95 54 1 44 138 13 102 9.35e-11 59.3

Find 78 sgRNAs with CRISPR-Local

Find 173 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CGAATCTCGGTTGCAGAATT+TGG 0.097656 1:+56473747 None:intergenic
CTGCCTCGTGATTATTATAC+TGG 0.247635 1:-56472623 MsG0180003095.01.T01:CDS
TCCAGGGAGCCAAGAGGTTT+TGG 0.247755 1:-56471915 MsG0180003095.01.T01:intron
CTGGAATATCGCCTTCTTCT+TGG 0.287567 1:+56471510 None:intergenic
CATGATCCATTTGGTCAGTT+TGG 0.300586 1:-56472665 MsG0180003095.01.T01:CDS
CTCCTATACCTTTCGTCTCT+TGG 0.302892 1:+56471379 None:intergenic
TGCAATCTTTGGCCAGGTTT+CGG 0.322049 1:+56473171 None:intergenic
AATCCATTACCTGCAATCTT+TGG 0.324397 1:+56473160 None:intergenic
GCTATAGAGGTGGCCGTGAT+AGG 0.332278 1:-56473217 MsG0180003095.01.T01:CDS
AATGGATCATGCAGATCTTC+TGG 0.338139 1:+56472677 None:intergenic
GATGCATAAGGCGATCTATA+AGG 0.339853 1:+56471325 None:intergenic
ATGGATGGTCAAGTTCTTCT+TGG 0.359588 1:-56471843 MsG0180003095.01.T01:CDS
CAAGAAGAAGGCGATATTCC+AGG 0.385912 1:-56471509 MsG0180003095.01.T01:intron
CTGTAACTCCTACCTCGATC+AGG 0.399237 1:+56471295 None:intergenic
ATGCATAAGGCGATCTATAA+GGG 0.402143 1:+56471326 None:intergenic
AGGAGCATAAGCCGCAGTCC+AGG 0.435288 1:-56471271 MsG0180003095.01.T01:CDS
CTTGGTGAAGGTGTATAGTC+TGG 0.438795 1:+56471528 None:intergenic
GAAACCTGGCCAAAGATTGC+AGG 0.442156 1:-56473169 MsG0180003095.01.T01:intron
GAAGATCTGCATGATCCATT+TGG 0.449391 1:-56472674 MsG0180003095.01.T01:CDS
CACCAAGAGACGAAAGGTAT+AGG 0.455985 1:-56471381 MsG0180003095.01.T01:CDS
TTCGCATCTGCAGCATCAGC+TGG 0.460121 1:+56471873 None:intergenic
CGTTTCGCAATTTCTGAACC+AGG 0.462288 1:-56473726 MsG0180003095.01.T01:intron
ACAGGACCAAACTGACCAAA+TGG 0.467960 1:+56472659 None:intergenic
CCTCCACCGTAACGCCTTGG+AGG 0.475266 1:+56473281 None:intergenic
CCATACCGGCCTCTCGGACT+CGG 0.481752 1:+56473245 None:intergenic
CTTCCTCCACCGTAACGCCT+TGG 0.483196 1:+56473278 None:intergenic
CCTTCACCTCCAAGGCGTTA+CGG 0.489909 1:-56473287 MsG0180003095.01.T01:CDS
TAGTCTTCTCGTCCGAAACC+TGG 0.495916 1:-56473183 MsG0180003095.01.T01:CDS
TGGTCAGTTTGGTCCTGTCA+AGG 0.499375 1:-56472654 MsG0180003095.01.T01:CDS
ATTACCTGCAATCTTTGGCC+AGG 0.501139 1:+56473165 None:intergenic
CCTCCAAGGCGTTACGGTGG+AGG 0.503424 1:-56473281 MsG0180003095.01.T01:CDS
TGAACAAACCCAAAACCTCT+TGG 0.503504 1:+56471906 None:intergenic
CCGAGTCCGAGAGGCCGGTA+TGG 0.507169 1:-56473245 MsG0180003095.01.T01:CDS
TCTCTTGCTCTCATTTCTTG+AGG 0.507948 1:+56471777 None:intergenic
TCAGGGCTTCTTGATGCATA+AGG 0.514015 1:+56471313 None:intergenic
AGAAATTGCGAAACGAATCT+CGG 0.514950 1:+56473734 None:intergenic
CGGTATGGAGGACGCTATAG+AGG 0.516782 1:-56473230 MsG0180003095.01.T01:CDS
CTATAGAGGTGGCCGTGATA+GGG 0.518683 1:-56473216 MsG0180003095.01.T01:CDS
GGAGCATAAGCCGCAGTCCA+GGG 0.527357 1:-56471270 MsG0180003095.01.T01:intron
GAAATGAGAGCAAGAGAACG+TGG 0.534870 1:-56471771 MsG0180003095.01.T01:CDS
CGTCCTCCATACCGGCCTCT+CGG 0.540189 1:+56473239 None:intergenic
GGTGTAGGTCATATGATCAT+AGG 0.540322 1:-56471608 MsG0180003095.01.T01:intron
GCAGTCCGAGTCCGAGAGGC+CGG 0.541406 1:-56473250 MsG0180003095.01.T01:CDS
TATCGCCTTCTTCTTGGTGA+AGG 0.551340 1:+56471516 None:intergenic
ATACACCTTCACCAAGAAGA+AGG 0.558214 1:-56471521 MsG0180003095.01.T01:CDS
GGCAGGTAAACATCCTTGAC+AGG 0.559386 1:+56472641 None:intergenic
CTCTATAGCGTCCTCCATAC+CGG 0.562836 1:+56473231 None:intergenic
CAGTATAATAATCACGAGGC+AGG 0.564750 1:+56472624 None:intergenic
TTTCGGACGAGAAGACTAGT+AGG 0.567398 1:+56473188 None:intergenic
CCGTAACGCCTTGGAGGTGA+AGG 0.570827 1:+56473287 None:intergenic
TGTAACTCCTACCTCGATCA+GGG 0.584139 1:+56471296 None:intergenic
GTGTAACGAGATCAAGATGA+GGG 0.587935 1:-56473330 MsG0180003095.01.T01:exon
AATAAAAGAACACAGTCAAG+AGG 0.594339 1:+56471063 None:intergenic
ATCGAGGTAGGAGTTACAGC+AGG 0.595558 1:-56471291 MsG0180003095.01.T01:CDS
CAAGGCGTTACGGTGGAGGA+AGG 0.596531 1:-56473277 MsG0180003095.01.T01:CDS
CAAGAAGCCCTGATCGAGGT+AGG 0.602469 1:-56471303 MsG0180003095.01.T01:CDS
AGTCCGAGAGGCCGGTATGG+AGG 0.609006 1:-56473242 MsG0180003095.01.T01:CDS
TGCCTCGTGATTATTATACT+GGG 0.615072 1:-56472622 MsG0180003095.01.T01:intron
GATGCGAAATATCACATGGA+TGG 0.617413 1:-56471858 MsG0180003095.01.T01:CDS
GTTACAGTCCTTCACCTCCA+AGG 0.628562 1:-56473295 MsG0180003095.01.T01:CDS
TAGTAGGCAAATCCCTATCA+CGG 0.635131 1:+56473204 None:intergenic
AGACGCAGTCCGAGTCCGAG+AGG 0.645705 1:-56473254 MsG0180003095.01.T01:CDS
ACCTAGAGTACCCTGGACTG+CGG 0.646462 1:+56471260 None:intergenic
TCTTGGTCGTGAGTTAACTG+TGG 0.656644 1:-56471826 MsG0180003095.01.T01:CDS
TGCATAAGGCGATCTATAAG+GGG 0.657366 1:+56471327 None:intergenic
TCACCTCCAAGGCGTTACGG+TGG 0.658178 1:-56473284 MsG0180003095.01.T01:CDS
CAATCTCACCAAGAGACGAA+AGG 0.661487 1:-56471387 MsG0180003095.01.T01:CDS
AAATGAGAGCAAGAGAACGT+GGG 0.674191 1:-56471770 MsG0180003095.01.T01:CDS
GAATAAGTTCGTGCATAGCG+TGG 0.675689 1:+56471558 None:intergenic
GGTGTAACGAGATCAAGATG+AGG 0.676629 1:-56473331 MsG0180003095.01.T01:exon
TGAGAGCAAGAGAACGTGGG+AGG 0.684897 1:-56471767 MsG0180003095.01.T01:intron
GCATCAAGAAGCCCTGATCG+AGG 0.697503 1:-56471307 MsG0180003095.01.T01:CDS
CACCCAGTATAATAATCACG+AGG 0.699045 1:+56472620 None:intergenic
TATGGAGGACGCTATAGAGG+TGG 0.702704 1:-56473227 MsG0180003095.01.T01:CDS
GGTGAACGAGAACGACGACG+TGG 0.702986 1:+56471579 None:intergenic
TTATGTTCCAGGGAGCCAAG+AGG 0.716843 1:-56471921 MsG0180003095.01.T01:intron
TGTAACGAGATCAAGATGAG+GGG 0.731365 1:-56473329 MsG0180003095.01.T01:exon
TGCAGATGCGAAATATCACA+TGG 0.740544 1:-56471862 MsG0180003095.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATAGTTTTATCAATTAATTA+AGG - Chr1:56471436-56471455 MsG0180003095.01.T01:intron 10.0%
!! AATCAGATGAATATAAAAAT+CGG + Chr1:56471143-56471162 None:intergenic 15.0%
!! TACTATATTAATACAATTTC+AGG + Chr1:56471932-56471951 None:intergenic 15.0%
!! TATGCTATTATTAAAATGTT+AGG + Chr1:56472028-56472047 None:intergenic 15.0%
!!! TTTTTTGTAAAATTTTATGG+TGG - Chr1:56472397-56472416 MsG0180003095.01.T01:intron 15.0%
!! AACACAAATCATAACATTAA+TGG + Chr1:56471275-56471294 None:intergenic 20.0%
!! AATAGCATAAACTAAATTGA+AGG - Chr1:56472039-56472058 MsG0180003095.01.T01:intron 20.0%
!! AGATGATATGTTAATTTATC+TGG - Chr1:56473680-56473699 MsG0180003095.01.T01:intron 20.0%
!! ATATAGTAAATAGTCCTTTA+AGG - Chr1:56471944-56471963 MsG0180003095.01.T01:intron 20.0%
!! ATCAAAGATAATATAAGTGT+TGG - Chr1:56473118-56473137 MsG0180003095.01.T01:intron 20.0%
!! ATTTATAACCCAATCATTTA+TGG - Chr1:56472464-56472483 MsG0180003095.01.T01:intron 20.0%
!! CAGAAATAAAATGAAAACTA+CGG + Chr1:56473633-56473652 None:intergenic 20.0%
!! TATAGTAAATAGTCCTTTAA+GGG - Chr1:56471945-56471964 MsG0180003095.01.T01:intron 20.0%
!! TGAATTTGATGAATTAAAGT+TGG - Chr1:56471372-56471391 MsG0180003095.01.T01:CDS 20.0%
!! TTTATAACCCAATCATTTAT+GGG - Chr1:56472465-56472484 MsG0180003095.01.T01:intron 20.0%
!!! AGATTTTCATCTCAATATTT+TGG - Chr1:56472700-56472719 MsG0180003095.01.T01:intron 20.0%
!!! TAAGAGATTTTTTTTGTTGT+GGG - Chr1:56471463-56471482 MsG0180003095.01.T01:intron 20.0%
!!! TTAAGAGATTTTTTTTGTTG+TGG - Chr1:56471462-56471481 MsG0180003095.01.T01:intron 20.0%
! GACAGATTTACATATACTTT+AGG + Chr1:56471992-56472011 None:intergenic 25.0%
! GTGTTTATTATTGAATTCTG+TGG - Chr1:56472747-56472766 MsG0180003095.01.T01:intron 25.0%
!!! ACTGTTTATTGATAATGAAG+TGG + Chr1:56472443-56472462 None:intergenic 25.0%
!!! GGTTTTTCAAATGTTATACT+TGG - Chr1:56472121-56472140 MsG0180003095.01.T01:intron 25.0%
AATAAAAGAACACAGTCAAG+AGG + Chr1:56473769-56473788 None:intergenic 30.0%
ACCAAATTCCCATAAATGAT+TGG + Chr1:56472476-56472495 None:intergenic 30.0%
AGAAACAAAATAGGCTATGA+AGG + Chr1:56472269-56472288 None:intergenic 30.0%
CATTTAGATCTTATGTTCCA+GGG - Chr1:56472898-56472917 MsG0180003095.01.T01:intron 30.0%
CCAAATTCCCATAAATGATT+GGG + Chr1:56472475-56472494 None:intergenic 30.0%
GAGATTGACCTGAATTATAA+TGG + Chr1:56473429-56473448 None:intergenic 30.0%
TAGGACTAGATTAACAGATT+TGG + Chr1:56471973-56471992 None:intergenic 30.0%
TGACATTTACTAGACTGATT+TGG - Chr1:56472100-56472119 MsG0180003095.01.T01:intron 30.0%
TGTGTTTAATGTTTATGTCC+TGG - Chr1:56472360-56472379 MsG0180003095.01.T01:intron 30.0%
TGTTGTGGGATGATATATAT+AGG - Chr1:56471477-56471496 MsG0180003095.01.T01:intron 30.0%
TTGAATAATCCATGCATGTT+TGG - Chr1:56471774-56471793 MsG0180003095.01.T01:CDS 30.0%
! ACTTGCAAGATTGGTTAATA+TGG - Chr1:56473598-56473617 MsG0180003095.01.T01:intron 30.0%
! CTTGCAAGATTGGTTAATAT+GGG - Chr1:56473599-56473618 MsG0180003095.01.T01:intron 30.0%
! GGATTTCAGAATTTTGTAGT+TGG - Chr1:56471687-56471706 MsG0180003095.01.T01:intron 30.0%
! GTGACTTAAGTCTTTATTTC+TGG - Chr1:56473362-56473381 MsG0180003095.01.T01:intron 30.0%
! TAAGTTTTCCAGAATCATAG+TGG + Chr1:56471858-56471877 None:intergenic 30.0%
!! AAATGTTATACTTGGTTTGC+AGG - Chr1:56472129-56472148 MsG0180003095.01.T01:intron 30.0%
!! ATTGATAATGAAGTGGCAAT+AGG + Chr1:56472436-56472455 None:intergenic 30.0%
!!! TCAATATTTTGGCTTCTACA+AGG - Chr1:56472711-56472730 MsG0180003095.01.T01:intron 30.0%
!!! TTTTGGGTTTGTTCAATTTG+TGG - Chr1:56472931-56472950 MsG0180003095.01.T01:intron 30.0%
AATCCATTACCTGCAATCTT+TGG + Chr1:56471672-56471691 None:intergenic 35.0%
ACACCAGAGATAGATTGAAT+AGG + Chr1:56473206-56473225 None:intergenic 35.0%
AGAAATTGCGAAACGAATCT+CGG + Chr1:56471098-56471117 None:intergenic 35.0%
AGTAGTAAGAGTTGAAGTTC+AGG - Chr1:56472603-56472622 MsG0180003095.01.T01:intron 35.0%
AGTTAATCCAGTAACAAACC+TGG + Chr1:56473341-56473360 None:intergenic 35.0%
ATAATGAAGTGGCAATAGGA+GGG + Chr1:56472432-56472451 None:intergenic 35.0%
ATCTAAATGCATCAAGTGAC+AGG + Chr1:56472887-56472906 None:intergenic 35.0%
ATGCATAAGGCGATCTATAA+GGG + Chr1:56473506-56473525 None:intergenic 35.0%
ATTCAATCTATCTCTGGTGT+AGG - Chr1:56473206-56473225 MsG0180003095.01.T01:CDS 35.0%
CCATGGTAAGAAACAAATAC+AGG + Chr1:56473400-56473419 None:intergenic 35.0%
CCCAATCATTTATGGGAATT+TGG - Chr1:56472472-56472491 MsG0180003095.01.T01:intron 35.0%
CTTCAACTCTTACTACTACT+TGG + Chr1:56472600-56472619 None:intergenic 35.0%
GCATTTAGATCTTATGTTCC+AGG - Chr1:56472897-56472916 MsG0180003095.01.T01:intron 35.0%
GGCATCTCAAGAAACAAAAT+AGG + Chr1:56472278-56472297 None:intergenic 35.0%
GTAGTAAGAGTTGAAGTTCA+GGG - Chr1:56472604-56472623 MsG0180003095.01.T01:intron 35.0%
TAAACAGCAAGAGTCAATTC+AGG + Chr1:56472299-56472318 None:intergenic 35.0%
TCTACAAGGTTAGAATTGTG+TGG - Chr1:56472725-56472744 MsG0180003095.01.T01:intron 35.0%
TCTCCTATTCAATCTATCTC+TGG - Chr1:56473200-56473219 MsG0180003095.01.T01:CDS 35.0%
TGCCTCGTGATTATTATACT+GGG - Chr1:56472207-56472226 MsG0180003095.01.T01:intron 35.0%
! AACAGATTTGGTACCCTTAA+AGG + Chr1:56471961-56471980 None:intergenic 35.0%
! AATTATAATGGAACAGGCCA+TGG + Chr1:56473417-56473436 None:intergenic 35.0%
! CCTGTATTTGTTTCTTACCA+TGG - Chr1:56473397-56473416 MsG0180003095.01.T01:intron 35.0%
! CTGGGTGAGTATATTTTCAT+TGG - Chr1:56472225-56472244 MsG0180003095.01.T01:intron 35.0%
! GACCTGAATTATAATGGAAC+AGG + Chr1:56473423-56473442 None:intergenic 35.0%
! TCGGAATTGAGAATTTTACC+TGG + Chr1:56471124-56471143 None:intergenic 35.0%
!! AAATCAGCGTTGATTCTGTA+AGG - Chr1:56471324-56471343 MsG0180003095.01.T01:CDS 35.0%
!! AGAGGCATTCTGATTAAAAC+AGG + Chr1:56473751-56473770 None:intergenic 35.0%
!! GGTGATTTAACTTGCAAGAT+TGG - Chr1:56473589-56473608 MsG0180003095.01.T01:intron 35.0%
AAAAAAATCCCAAGTCGTCC+AGG + Chr1:56472381-56472400 None:intergenic 40.0%
AAATGAGAGCAAGAGAACGT+GGG - Chr1:56473059-56473078 MsG0180003095.01.T01:intron 40.0%
AACGTGGGAGGTAAGAATAT+AGG - Chr1:56473074-56473093 MsG0180003095.01.T01:intron 40.0%
AATGGATCATGCAGATCTTC+TGG + Chr1:56472155-56472174 None:intergenic 40.0%
ACAATTCTGCCAAACATGCA+TGG + Chr1:56471786-56471805 None:intergenic 40.0%
ACAATTTCAGGACTCAATCG+AGG + Chr1:56471920-56471939 None:intergenic 40.0%
ATACACCTTCACCAAGAAGA+AGG - Chr1:56473308-56473327 MsG0180003095.01.T01:CDS 40.0%
ATGGATGGTCAAGTTCTTCT+TGG - Chr1:56472986-56473005 MsG0180003095.01.T01:intron 40.0%
CAAAAATATGATCGCGTCTC+CGG + Chr1:56472800-56472819 None:intergenic 40.0%
CAACTCTTACTACTACTTGG+TGG + Chr1:56472597-56472616 None:intergenic 40.0%
CACCCAGTATAATAATCACG+AGG + Chr1:56472212-56472231 None:intergenic 40.0%
CAGTATAATAATCACGAGGC+AGG + Chr1:56472208-56472227 None:intergenic 40.0%
CATGATCCATTTGGTCAGTT+TGG - Chr1:56472164-56472183 MsG0180003095.01.T01:intron 40.0%
CTGCCTCGTGATTATTATAC+TGG - Chr1:56472206-56472225 MsG0180003095.01.T01:intron 40.0%
GAAGATCTGCATGATCCATT+TGG - Chr1:56472155-56472174 MsG0180003095.01.T01:intron 40.0%
GATAATGAAGTGGCAATAGG+AGG + Chr1:56472433-56472452 None:intergenic 40.0%
GATGCATAAGGCGATCTATA+AGG + Chr1:56473507-56473526 None:intergenic 40.0%
GATGCGAAATATCACATGGA+TGG - Chr1:56472971-56472990 MsG0180003095.01.T01:intron 40.0%
GGCCTGTTCCATTATAATTC+AGG - Chr1:56473418-56473437 MsG0180003095.01.T01:intron 40.0%
GGTGTAGGTCATATGATCAT+AGG - Chr1:56473221-56473240 MsG0180003095.01.T01:CDS 40.0%
GTGTAACGAGATCAAGATGA+GGG - Chr1:56471499-56471518 MsG0180003095.01.T01:intron 40.0%
GTGTAGCTACAGCAAAATCA+TGG - Chr1:56471822-56471841 MsG0180003095.01.T01:CDS 40.0%
TAATGAAGTGGCAATAGGAG+GGG + Chr1:56472431-56472450 None:intergenic 40.0%
TAGCTACAGCAAAATCATGG+TGG - Chr1:56471825-56471844 MsG0180003095.01.T01:CDS 40.0%
TAGTAGGCAAATCCCTATCA+CGG + Chr1:56471628-56471647 None:intergenic 40.0%
TCTCTTGCTCTCATTTCTTG+AGG + Chr1:56473055-56473074 None:intergenic 40.0%
TGAACAAACCCAAAACCTCT+TGG + Chr1:56472926-56472945 None:intergenic 40.0%
TGAGTTCAAACTGAACCTTC+CGG + Chr1:56472643-56472662 None:intergenic 40.0%
TGCAGATGCGAAATATCACA+TGG - Chr1:56472967-56472986 MsG0180003095.01.T01:intron 40.0%
TGCATAAGGCGATCTATAAG+GGG + Chr1:56473505-56473524 None:intergenic 40.0%
TGTAACGAGATCAAGATGAG+GGG - Chr1:56471500-56471519 MsG0180003095.01.T01:intron 40.0%
TTTAGTGACTAGCGCATGTA+TGG + Chr1:56471887-56471906 None:intergenic 40.0%
! TTAAATCACCTAGAGTACCC+TGG + Chr1:56473579-56473598 None:intergenic 40.0%
! TTTCCAGAATCATAGTGGAG+CGG + Chr1:56471853-56471872 None:intergenic 40.0%
!! CGATATTCCAGGTTTGTTAC+TGG - Chr1:56473331-56473350 MsG0180003095.01.T01:exon 40.0%
ACAGGACCAAACTGACCAAA+TGG + Chr1:56472173-56472192 None:intergenic 45.0%
ACCTGCATACTGAAGTAGTG+TGG + Chr1:56472842-56472861 None:intergenic 45.0%
ATTACCTGCAATCTTTGGCC+AGG + Chr1:56471667-56471686 None:intergenic 45.0%
CAAGAAGAAGGCGATATTCC+AGG - Chr1:56473320-56473339 MsG0180003095.01.T01:exon 45.0%
CAATCTCACCAAGAGACGAA+AGG - Chr1:56473442-56473461 MsG0180003095.01.T01:intron 45.0%
CCTGCATACTGAAGTAGTGT+GGG + Chr1:56472841-56472860 None:intergenic 45.0%
CGAATCTCGGTTGCAGAATT+TGG + Chr1:56471085-56471104 None:intergenic 45.0%
CGTTTCGCAATTTCTGAACC+AGG - Chr1:56471103-56471122 MsG0180003095.01.T01:intron 45.0%
CTCCTATACCTTTCGTCTCT+TGG + Chr1:56473453-56473472 None:intergenic 45.0%
CTGGAATATCGCCTTCTTCT+TGG + Chr1:56473322-56473341 None:intergenic 45.0%
GAAATGAGAGCAAGAGAACG+TGG - Chr1:56473058-56473077 MsG0180003095.01.T01:intron 45.0%
GAATAAGTTCGTGCATAGCG+TGG + Chr1:56473274-56473293 None:intergenic 45.0%
GGTGTAACGAGATCAAGATG+AGG - Chr1:56471498-56471517 MsG0180003095.01.T01:intron 45.0%
GTTTATGTCCTGGACGACTT+GGG - Chr1:56472370-56472389 MsG0180003095.01.T01:intron 45.0%
TATCGCCTTCTTCTTGGTGA+AGG + Chr1:56473316-56473335 None:intergenic 45.0%
TCAGGGCTTCTTGATGCATA+AGG + Chr1:56473519-56473538 None:intergenic 45.0%
TCAGGGTGATGTTAGTGTTC+CGG - Chr1:56472621-56472640 MsG0180003095.01.T01:intron 45.0%
TCTTGGTCGTGAGTTAACTG+TGG - Chr1:56473003-56473022 MsG0180003095.01.T01:intron 45.0%
TGCAATCTTTGGCCAGGTTT+CGG + Chr1:56471661-56471680 None:intergenic 45.0%
TGGCCAAAGATTGCAGGTAA+TGG - Chr1:56471666-56471685 MsG0180003095.01.T01:intron 45.0%
TGTAACTCCTACCTCGATCA+GGG + Chr1:56473536-56473555 None:intergenic 45.0%
TGTTTATGTCCTGGACGACT+TGG - Chr1:56472369-56472388 MsG0180003095.01.T01:intron 45.0%
TTTCGGACGAGAAGACTAGT+AGG + Chr1:56471644-56471663 None:intergenic 45.0%
! CACCAAGAGACGAAAGGTAT+AGG - Chr1:56473448-56473467 MsG0180003095.01.T01:intron 45.0%
! CTTGGTGAAGGTGTATAGTC+TGG + Chr1:56473304-56473323 None:intergenic 45.0%
! GAGTGTGACTTGTGATTGTC+CGG - Chr1:56472778-56472797 MsG0180003095.01.T01:intron 45.0%
!!! TTTTTTTTTGTAAAATTTTA+TGG - Chr1:56472394-56472413 MsG0180003095.01.T01:intron 5.0%
ATCGAGGTAGGAGTTACAGC+AGG - Chr1:56473538-56473557 MsG0180003095.01.T01:intron 50.0%
CCCACACTACTTCAGTATGC+AGG - Chr1:56472838-56472857 MsG0180003095.01.T01:intron 50.0%
CTATAGAGGTGGCCGTGATA+GGG - Chr1:56471613-56471632 MsG0180003095.01.T01:intron 50.0%
CTCTATAGCGTCCTCCATAC+CGG + Chr1:56471601-56471620 None:intergenic 50.0%
CTGTAACTCCTACCTCGATC+AGG + Chr1:56473537-56473556 None:intergenic 50.0%
GAAACCTGGCCAAAGATTGC+AGG - Chr1:56471660-56471679 MsG0180003095.01.T01:intron 50.0%
GGCAGGTAAACATCCTTGAC+AGG + Chr1:56472191-56472210 None:intergenic 50.0%
GGTGATGTTAGTGTTCCGGA+AGG - Chr1:56472625-56472644 MsG0180003095.01.T01:CDS 50.0%
GTTACAGTCCTTCACCTCCA+AGG - Chr1:56471534-56471553 MsG0180003095.01.T01:CDS 50.0%
TAGTCTTCTCGTCCGAAACC+TGG - Chr1:56471646-56471665 MsG0180003095.01.T01:intron 50.0%
TATGGAGGACGCTATAGAGG+TGG - Chr1:56471602-56471621 MsG0180003095.01.T01:CDS 50.0%
TGGTCAGTTTGGTCCTGTCA+AGG - Chr1:56472175-56472194 MsG0180003095.01.T01:intron 50.0%
TTATGTTCCAGGGAGCCAAG+AGG - Chr1:56472908-56472927 MsG0180003095.01.T01:intron 50.0%
CAAGAAGCCCTGATCGAGGT+AGG - Chr1:56473526-56473545 MsG0180003095.01.T01:intron 55.0%
CGGTATGGAGGACGCTATAG+AGG - Chr1:56471599-56471618 MsG0180003095.01.T01:CDS 55.0%
GCATCAAGAAGCCCTGATCG+AGG - Chr1:56473522-56473541 MsG0180003095.01.T01:intron 55.0%
GCTATAGAGGTGGCCGTGAT+AGG - Chr1:56471612-56471631 MsG0180003095.01.T01:intron 55.0%
GCTCCGCTCCACTATGATTC+TGG - Chr1:56471847-56471866 MsG0180003095.01.T01:CDS 55.0%
TGAGAGCAAGAGAACGTGGG+AGG - Chr1:56473062-56473081 MsG0180003095.01.T01:intron 55.0%
TTCGCATCTGCAGCATCAGC+TGG + Chr1:56472959-56472978 None:intergenic 55.0%
! ACCTAGAGTACCCTGGACTG+CGG + Chr1:56473572-56473591 None:intergenic 55.0%
! CCAGGGAGCCAAGAGGTTTT+GGG - Chr1:56472915-56472934 MsG0180003095.01.T01:intron 55.0%
! CCTTCACCTCCAAGGCGTTA+CGG - Chr1:56471542-56471561 MsG0180003095.01.T01:CDS 55.0%
! TCCAGGGAGCCAAGAGGTTT+TGG - Chr1:56472914-56472933 MsG0180003095.01.T01:intron 55.0%
CCCAAAACCTCTTGGCTCCC+TGG + Chr1:56472918-56472937 None:intergenic 60.0%
CCGTAACGCCTTGGAGGTGA+AGG + Chr1:56471545-56471564 None:intergenic 60.0%
CTTCCTCCACCGTAACGCCT+TGG + Chr1:56471554-56471573 None:intergenic 60.0%
GGAGCATAAGCCGCAGTCCA+GGG - Chr1:56473559-56473578 MsG0180003095.01.T01:intron 60.0%
GGTGAACGAGAACGACGACG+TGG + Chr1:56473253-56473272 None:intergenic 60.0%
! AGGAGCATAAGCCGCAGTCC+AGG - Chr1:56473558-56473577 MsG0180003095.01.T01:intron 60.0%
! CAAGGCGTTACGGTGGAGGA+AGG - Chr1:56471552-56471571 MsG0180003095.01.T01:CDS 60.0%
! TCACCTCCAAGGCGTTACGG+TGG - Chr1:56471545-56471564 MsG0180003095.01.T01:CDS 60.0%
AGACGCAGTCCGAGTCCGAG+AGG - Chr1:56471575-56471594 MsG0180003095.01.T01:CDS 65.0%
AGTCCGAGAGGCCGGTATGG+AGG - Chr1:56471587-56471606 MsG0180003095.01.T01:CDS 65.0%
CCATACCGGCCTCTCGGACT+CGG + Chr1:56471587-56471606 None:intergenic 65.0%
CCTCCACCGTAACGCCTTGG+AGG + Chr1:56471551-56471570 None:intergenic 65.0%
CGTCCTCCATACCGGCCTCT+CGG + Chr1:56471593-56471612 None:intergenic 65.0%
! CCTCCAAGGCGTTACGGTGG+AGG - Chr1:56471548-56471567 MsG0180003095.01.T01:CDS 65.0%
! GCCGCAGTCCAGGGTACTCT+AGG - Chr1:56473568-56473587 MsG0180003095.01.T01:intron 65.0%
CCGAGTCCGAGAGGCCGGTA+TGG - Chr1:56471584-56471603 MsG0180003095.01.T01:CDS 70.0%
GCAGTCCGAGTCCGAGAGGC+CGG - Chr1:56471579-56471598 MsG0180003095.01.T01:CDS 70.0%
Chromosome Type Strat End Strand Name
Chr1 gene 56471035 56473816 56471035 ID=MsG0180003095.01;Name=MsG0180003095.01
Chr1 mRNA 56471035 56473816 56471035 ID=MsG0180003095.01.T01;Parent=MsG0180003095.01;Name=MsG0180003095.01.T01;_AED=0.39;_eAED=0.39;_QI=106|0.83|0.71|1|0.83|0.71|7|0|239
Chr1 exon 56471035 56471089 56471035 ID=MsG0180003095.01.T01:exon:39452;Parent=MsG0180003095.01.T01
Chr1 exon 56471271 56471411 56471271 ID=MsG0180003095.01.T01:exon:39451;Parent=MsG0180003095.01.T01
Chr1 exon 56471510 56471623 56471510 ID=MsG0180003095.01.T01:exon:39450;Parent=MsG0180003095.01.T01
Chr1 exon 56471768 56471932 56471768 ID=MsG0180003095.01.T01:exon:39449;Parent=MsG0180003095.01.T01
Chr1 exon 56472623 56472700 56472623 ID=MsG0180003095.01.T01:exon:39448;Parent=MsG0180003095.01.T01
Chr1 exon 56473170 56473352 56473170 ID=MsG0180003095.01.T01:exon:39447;Parent=MsG0180003095.01.T01
Chr1 exon 56473727 56473816 56473727 ID=MsG0180003095.01.T01:exon:39446;Parent=MsG0180003095.01.T01
Chr1 five_prime_UTR 56473727 56473816 56473727 ID=MsG0180003095.01.T01:five_prime_utr;Parent=MsG0180003095.01.T01
Chr1 five_prime_UTR 56473337 56473352 56473337 ID=MsG0180003095.01.T01:five_prime_utr;Parent=MsG0180003095.01.T01
Chr1 CDS 56473170 56473336 56473170 ID=MsG0180003095.01.T01:cds;Parent=MsG0180003095.01.T01
Chr1 CDS 56472623 56472700 56472623 ID=MsG0180003095.01.T01:cds;Parent=MsG0180003095.01.T01
Chr1 CDS 56471768 56471932 56471768 ID=MsG0180003095.01.T01:cds;Parent=MsG0180003095.01.T01
Chr1 CDS 56471510 56471623 56471510 ID=MsG0180003095.01.T01:cds;Parent=MsG0180003095.01.T01
Chr1 CDS 56471271 56471411 56471271 ID=MsG0180003095.01.T01:cds;Parent=MsG0180003095.01.T01
Chr1 CDS 56471035 56471089 56471035 ID=MsG0180003095.01.T01:cds;Parent=MsG0180003095.01.T01
Gene Sequence

>MsG0180003095.01.T01

ATGAGGGGAAGAAGTTACAGTTACAGTCCTTCACCTCCAAGGCGTTACGGTGGAGGAAGGAGACGCAGTCCGAGTCCGAGAGGCCGGTATGGAGGACGCTATAGAGGTGGCCGTGATAGGGATTTGCCTACTAGTCTTCTCGTCCGAAACCTGGCCAAAGATTGCAGGCCAGAAGATCTGCATGATCCATTTGGTCAGTTTGGTCCTGTCAAGGATGTTTACCTGCCTCGTGATTATTATACTGGGGAGCCAAGAGGTTTTGGGTTTGTTCAATTTGTGGATCCAGCTGATGCTGCAGATGCGAAATATCACATGGATGGTCAAGTTCTTCTTGGTCGTGAGTTAACTGTGGTGTTTGCTGAAGAAAACAGAAAGAAACCTCAAGAAATGAGAGCAAGAGAACGTGGGAGGTCATATGATCATAGGCGATCTCCACGTCGTCGTTCTCGTTCACCACGCTATGCACGAACTTATTCTCATAGTCCAGACTATACACCTTCACCAAGAAGAAGGCGATATTCCAGGTCAATCTCACCAAGAGACGAAAGGTATAGGAGACGTTCATACTCTAGATCTACTTATAGATCCCCTTATAGATCGCCTTATGCATCAAGAAGCCCTGATCGAGGTAGGAGTTACAGCAGGAGCATAAGCCGCAGTCCAGGAATGCCTCTTGACTGTGTTCTTTTATTTGTGCTTAAGAATTTCATCGATGCTTAG
Protein sequence

>MsG0180003095.01.T01

MRGRSYSYSPSPPRRYGGGRRRSPSPRGRYGGRYRGGRDRDLPTSLLVRNLAKDCRPEDLHDPFGQFGPVKDVYLPRDYYTGEPRGFGFVQFVDPADAADAKYHMDGQVLLGRELTVVFAEENRKKPQEMRARERGRSYDHRRSPRRRSRSPRYARTYSHSPDYTPSPRRRRYSRSISPRDERYRRRSYSRSTYRSPYRSPYASRSPDRGRSYSRSISRSPGMPLDCVLLFVLKNFIDA*