AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0180003251.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180003251.01.T01 MTR_1g056890 99.310 145 1 0 1 145 1 145 1.05e-104 295
MsG0180003251.01.T01 MTR_6g090500 44.295 149 68 4 1 145 1 138 1.15e-33 115
MsG0180003251.01.T01 MTR_4g133090 44.295 149 68 4 1 145 1 138 1.15e-33 115
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180003251.01.T01 AT3G59600 61.702 141 53 1 5 145 7 146 3.40e-64 193
MsG0180003251.01.T01 AT1G54250 61.702 141 53 1 5 145 7 146 1.70e-63 191

Find 52 sgRNAs with CRISPR-Local

Find 258 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AAATTTCTGTCCCATCTTTA+AGG 0.163307 1:+59127120 None:intergenic
GATGATTTAAAACAAATATT+TGG 0.195820 1:-59124965 MsG0180003251.01.T01:three_prime_UTR
GGATTATAGTAGCCAGTATC+TGG 0.234479 1:+59127058 None:intergenic
CACTTCTTGAAGATATCTTT+AGG 0.284321 1:-59127361 MsG0180003251.01.T01:CDS
AGGGAGTTGAATATTTCTTT+TGG 0.310558 1:-59125180 MsG0180003251.01.T01:CDS
ACTTCTTGAAGATATCTTTA+GGG 0.337546 1:-59127360 MsG0180003251.01.T01:CDS
CCTGATGGCACCCCAGATAC+TGG 0.345604 1:-59127070 MsG0180003251.01.T01:CDS
CAGCATGACTATCTAGTTTG+TGG 0.355058 1:-59124869 MsG0180003251.01.T01:three_prime_UTR
AATTTCTGTCCCATCTTTAA+GGG 0.357752 1:+59127121 None:intergenic
GAGGAGGAACGGTGGTGGAT+AGG 0.368114 1:+59129024 None:intergenic
CACCGTTCCTCCTCTTCTCT+TGG 0.372762 1:-59129015 MsG0180003251.01.T01:intron
TCCCACCTCTCACTGATTCC+CGG 0.373185 1:-59129057 MsG0180003251.01.T01:five_prime_UTR
CCAGTATCTGGGGTGCCATC+AGG 0.417512 1:+59127070 None:intergenic
GTGGATAGGAGGAGCTGCGC+CGG 0.433526 1:+59129038 None:intergenic
AACTATGAATATGTCATGTA+TGG 0.442578 1:-59126387 MsG0180003251.01.T01:CDS
AAACAAGCTTTCTTCACTCT+TGG 0.445244 1:+59125054 None:intergenic
TACAGAGTAACAGAGGGTTC+AGG 0.455659 1:-59126357 MsG0180003251.01.T01:CDS
GATAGTCATGCTGCAATCCT+TGG 0.470526 1:+59124879 None:intergenic
GGTAATCATGAAGTAACTTC+CGG 0.471518 1:-59124944 MsG0180003251.01.T01:three_prime_UTR
TTGTTTCTAACATCAATGTA+TGG 0.476810 1:-59125037 MsG0180003251.01.T01:three_prime_UTR
TGAGCTATATCCCTTAAAGA+TGG 0.478515 1:-59127131 MsG0180003251.01.T01:CDS
GATTATAGTAGCCAGTATCT+GGG 0.489343 1:+59127059 None:intergenic
GAGGAACGGTGGTGGATAGG+AGG 0.498730 1:+59129027 None:intergenic
TGACTATCTAGTTTGTGGAT+TGG 0.499748 1:-59124864 MsG0180003251.01.T01:three_prime_UTR
TACCGTTTGTAATTCTAATG+TGG 0.528373 1:-59125004 MsG0180003251.01.T01:three_prime_UTR
AGATACTGGCTACTATAATC+CGG 0.532782 1:-59127056 MsG0180003251.01.T01:intron
TGGATAGGAGGAGCTGCGCC+GGG 0.537171 1:+59129039 None:intergenic
GAGAAGAGGAGGAACGGTGG+TGG 0.539747 1:+59129019 None:intergenic
CGCCGGGAATCAGTGAGAGG+TGG 0.541797 1:+59129055 None:intergenic
TGGGCTTCTGATGATGCTGA+AGG 0.547894 1:-59125157 MsG0180003251.01.T01:CDS
ATCCACATTAGAATTACAAA+CGG 0.550991 1:+59125002 None:intergenic
GAGCTATATCCCTTAAAGAT+GGG 0.563262 1:-59127130 MsG0180003251.01.T01:CDS
AGTGAGCTGAACTGCTACAA+TGG 0.571239 1:-59127390 MsG0180003251.01.T01:exon
AATCCTTGGTCTGATATCAC+AGG 0.579037 1:+59124893 None:intergenic
AGAGTAACAGAGGGTTCAGG+AGG 0.579301 1:-59126354 MsG0180003251.01.T01:CDS
ACAAGTTTGAGCTTGATCAA+AGG 0.582436 1:-59125116 MsG0180003251.01.T01:CDS
GCCGGGAATCAGTGAGAGGT+GGG 0.592745 1:+59129056 None:intergenic
TGACTCACCAAGAGAAGAGG+AGG 0.598727 1:+59129008 None:intergenic
GTGTGACTCACCAAGAGAAG+AGG 0.599795 1:+59129005 None:intergenic
GGGCTTCTGATGATGCTGAA+GGG 0.605774 1:-59125156 MsG0180003251.01.T01:CDS
AAAGGTTGTATCTTCTGATG+AGG 0.609382 1:-59125098 MsG0180003251.01.T01:CDS
CACCAAGAGAAGAGGAGGAA+CGG 0.616450 1:+59129013 None:intergenic
GTTCCAACACTCAATCCTGA+TGG 0.623194 1:-59127085 MsG0180003251.01.T01:CDS
CTGCGCCGGGAATCAGTGAG+AGG 0.629639 1:+59129052 None:intergenic
GTGCCATCAGGATTGAGTGT+TGG 0.642346 1:+59127082 None:intergenic
GAGCTGAACTGCTACAATGG+TGG 0.645893 1:-59127387 MsG0180003251.01.T01:exon
GAGTAACAGAGGGTTCAGGA+GGG 0.649006 1:-59126353 MsG0180003251.01.T01:CDS
AAGCTTTACAGAGTAACAGA+GGG 0.656174 1:-59126363 MsG0180003251.01.T01:CDS
AAAGCTTTACAGAGTAACAG+AGG 0.672431 1:-59126364 MsG0180003251.01.T01:CDS
ATTATAGTAGCCAGTATCTG+GGG 0.679143 1:+59127060 None:intergenic
CAAGAGAAGAGGAGGAACGG+TGG 0.695512 1:+59129016 None:intergenic
ATTCCTGTGATATCAGACCA+AGG 0.711879 1:-59124896 MsG0180003251.01.T01:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATGTTTAATTACCAAAAT+GGG - Chr1:59127835-59127854 MsG0180003251.01.T01:intron 15.0%
!! AAGATAAAATAAAGTTGAAT+TGG - Chr1:59125491-59125510 MsG0180003251.01.T01:intron 15.0%
!! AATTTCATAAGAAATAGAAA+CGG - Chr1:59128208-59128227 MsG0180003251.01.T01:intron 15.0%
!! CTATATAATAATCACTTAAT+AGG - Chr1:59127169-59127188 MsG0180003251.01.T01:CDS 15.0%
!! TAAATGTTTAATTACCAAAA+TGG - Chr1:59127834-59127853 MsG0180003251.01.T01:intron 15.0%
!!! AAATATTGTTTCTTGATTTT+AGG - Chr1:59125365-59125384 MsG0180003251.01.T01:intron 15.0%
!!! AGCATTTCTTAATATTTTTA+GGG + Chr1:59125984-59126003 None:intergenic 15.0%
!!! ATTTGAAATTTTGAGAAAAT+TGG + Chr1:59125913-59125932 None:intergenic 15.0%
!!! CACTTTTTTTTAAATATCAT+AGG + Chr1:59128081-59128100 None:intergenic 15.0%
!!! GATGATTTAAAACAAATATT+TGG - Chr1:59128965-59128984 MsG0180003251.01.T01:intron 15.0%
!!! TGTTCTTAATATATTACTTT+TGG - Chr1:59128726-59128745 MsG0180003251.01.T01:intron 15.0%
!!! TTTTATCTATAAGATATGTT+AGG + Chr1:59125734-59125753 None:intergenic 15.0%
!! AAATAAGTTCAAATATCTTG+AGG + Chr1:59127023-59127042 None:intergenic 20.0%
!! AATAAGTTCAAAATAGCTTA+TGG + Chr1:59127057-59127076 None:intergenic 20.0%
!! AGTACTATAATGTTAATCAA+TGG - Chr1:59128588-59128607 MsG0180003251.01.T01:intron 20.0%
!! GAAACTTAAAAATAAGGTAA+AGG + Chr1:59126520-59126539 None:intergenic 20.0%
!! TCTTAAAAAAAAACTCTAAC+CGG + Chr1:59128635-59128654 None:intergenic 20.0%
!!! ACATTGTTTCTGTAAAAAAA+AGG - Chr1:59127991-59128010 MsG0180003251.01.T01:intron 20.0%
!!! ACATTTGTTAATTGAGATTT+TGG - Chr1:59125254-59125273 MsG0180003251.01.T01:intron 20.0%
!!! AGTATGTATGTATTTGTTTT+AGG - Chr1:59127878-59127897 MsG0180003251.01.T01:intron 20.0%
!!! ATTTGTTTTAAATCATCATC+AGG + Chr1:59128962-59128981 None:intergenic 20.0%
!!! CAATTTTATTTTGCATTACT+TGG + Chr1:59127403-59127422 None:intergenic 20.0%
!!! CAGCATTTCTTAATATTTTT+AGG + Chr1:59125985-59126004 None:intergenic 20.0%
!!! CTTTTATTCAATTTAAGTGT+TGG - Chr1:59126030-59126049 MsG0180003251.01.T01:intron 20.0%
!!! GCTAATTTTTTTATTTTCCA+GGG - Chr1:59127498-59127517 MsG0180003251.01.T01:intron 20.0%
!!! TAATATTTTTAGGGTATGTT+TGG + Chr1:59125975-59125994 None:intergenic 20.0%
!!! TATTTTTCGATTCTTTTAGT+GGG - Chr1:59126651-59126670 MsG0180003251.01.T01:intron 20.0%
!!! TGCTAATTTTTTTATTTTCC+AGG - Chr1:59127497-59127516 MsG0180003251.01.T01:intron 20.0%
!!! TTAATATATTACTTTTGGCA+GGG - Chr1:59128731-59128750 MsG0180003251.01.T01:intron 20.0%
!!! TTATTTTTCGATTCTTTTAG+TGG - Chr1:59126650-59126669 MsG0180003251.01.T01:intron 20.0%
!!! TTTTATCAATTCATGTTGTT+TGG - Chr1:59127788-59127807 MsG0180003251.01.T01:intron 20.0%
! AACTATGAATATGTCATGTA+TGG - Chr1:59127543-59127562 MsG0180003251.01.T01:intron 25.0%
! AATATTAAGAAATGCTGTCT+TGG - Chr1:59125988-59126007 MsG0180003251.01.T01:intron 25.0%
! AATCAAATAACCCAATTCAA+CGG - Chr1:59125752-59125771 MsG0180003251.01.T01:intron 25.0%
! ACGATTAATGAATGTAATAC+CGG + Chr1:59126896-59126915 None:intergenic 25.0%
! ACTTAAAAATAAGGTAAAGG+TGG + Chr1:59126517-59126536 None:intergenic 25.0%
! ACTTCTTGAAGATATCTTTA+GGG - Chr1:59126570-59126589 MsG0180003251.01.T01:intron 25.0%
! AGTAGAGAATGAAATAAAGT+AGG + Chr1:59126316-59126335 None:intergenic 25.0%
! ATAAGATATGTTAGGCTTTA+CGG + Chr1:59125726-59125745 None:intergenic 25.0%
! ATCCACATTAGAATTACAAA+CGG + Chr1:59128931-59128950 None:intergenic 25.0%
! CTCACTGAAACTTAAAAATA+AGG + Chr1:59126526-59126545 None:intergenic 25.0%
! CTTAAAAAAAAACTCTAACC+GGG + Chr1:59128634-59128653 None:intergenic 25.0%
! GACAAAAAGTTATTCGAAAA+AGG - Chr1:59126613-59126632 MsG0180003251.01.T01:intron 25.0%
! GACTAAATTGAGAAATCAAA+TGG - Chr1:59128278-59128297 MsG0180003251.01.T01:intron 25.0%
! GTAGAGAATGAAATAAAGTA+GGG + Chr1:59126315-59126334 None:intergenic 25.0%
! TAAACCAAAGACAAATCAAA+AGG + Chr1:59126487-59126506 None:intergenic 25.0%
! TAACCAAATTTAGTACAGAT+AGG + Chr1:59127337-59127356 None:intergenic 25.0%
! TATAAAACAAACTGTTACTG+CGG - Chr1:59128117-59128136 MsG0180003251.01.T01:intron 25.0%
!! AATATCATAGGTTTTTCTAG+AGG + Chr1:59128069-59128088 None:intergenic 25.0%
!! AGTTGAATATTTCTTTTGGT+GGG - Chr1:59128754-59128773 MsG0180003251.01.T01:intron 25.0%
!! TCTAGAGGTTTTTATATCAA+AGG + Chr1:59128054-59128073 None:intergenic 25.0%
!! TGACAGAATTGATTTATGTT+TGG - Chr1:59127744-59127763 MsG0180003251.01.T01:intron 25.0%
!! TTTTTGTAGATAATTTCTGC+CGG + Chr1:59129008-59129027 None:intergenic 25.0%
!!! AAAGATAAAAGTGTTTAGGT+TGG + Chr1:59128185-59128204 None:intergenic 25.0%
!!! ATTTTGGTGCTTTTGTTTAT+TGG - Chr1:59125270-59125289 MsG0180003251.01.T01:intron 25.0%
!!! CAAAGAAAATTGATTGGTTT+GGG - Chr1:59126227-59126246 MsG0180003251.01.T01:intron 25.0%
!!! CGGTTTTAGTGTTAAAAATA+CGG - Chr1:59126184-59126203 MsG0180003251.01.T01:intron 25.0%
!!! CTTAATATATTACTTTTGGC+AGG - Chr1:59128730-59128749 MsG0180003251.01.T01:intron 25.0%
!!! GGTTTTAGTGTTAAAAATAC+GGG - Chr1:59126185-59126204 MsG0180003251.01.T01:intron 25.0%
!!! TTGCAAAGATAAAAGTGTTT+AGG + Chr1:59128189-59128208 None:intergenic 25.0%
!!! TTGTTTCTAACATCAATGTA+TGG - Chr1:59128893-59128912 MsG0180003251.01.T01:intron 25.0%
!!! TTTTGGTGCTTTTGTTTATT+GGG - Chr1:59125271-59125290 MsG0180003251.01.T01:intron 25.0%
!!! TTTTTATTTTCCAGGGTAAT+CGG - Chr1:59127505-59127524 MsG0180003251.01.T01:intron 25.0%
AAATTTCTGTCCCATCTTTA+AGG + Chr1:59126813-59126832 None:intergenic 30.0%
AATTTCTGTCCCATCTTTAA+GGG + Chr1:59126812-59126831 None:intergenic 30.0%
AGGAAAAAGGGTTGAAATAT+TGG + Chr1:59124962-59124981 None:intergenic 30.0%
AGGGATTTAGTAATTTACCA+AGG + Chr1:59126296-59126315 None:intergenic 30.0%
ATTACTTGGTTAAGAGCAAT+AGG + Chr1:59127389-59127408 None:intergenic 30.0%
CACAAAACTTAGTATGTGTT+TGG - Chr1:59125632-59125651 MsG0180003251.01.T01:intron 30.0%
CACTTCTTGAAGATATCTTT+AGG - Chr1:59126569-59126588 MsG0180003251.01.T01:intron 30.0%
CATAAGTTGTTTATCCAAAC+AGG + Chr1:59125392-59125411 None:intergenic 30.0%
CCAAAAACAAAATACAACCT+TGG - Chr1:59128024-59128043 MsG0180003251.01.T01:intron 30.0%
GGAAAAAGGGTTGAAATATT+GGG + Chr1:59124961-59124980 None:intergenic 30.0%
GGGATTTAGTAATTTACCAA+GGG + Chr1:59126295-59126314 None:intergenic 30.0%
TACCGTTTGTAATTCTAATG+TGG - Chr1:59128926-59128945 MsG0180003251.01.T01:intron 30.0%
TGAGTTTGACAGAATTGATT+TGG - Chr1:59127708-59127727 MsG0180003251.01.T01:intron 30.0%
TTACTGCGGAAAAAATGAAT+GGG - Chr1:59128131-59128150 MsG0180003251.01.T01:intron 30.0%
TTAGTGTTAAAAATACGGGT+TGG - Chr1:59126189-59126208 MsG0180003251.01.T01:intron 30.0%
TTTATATCAAAGGTAAGCCA+AGG + Chr1:59128044-59128063 None:intergenic 30.0%
! AGGGAGTTGAATATTTCTTT+TGG - Chr1:59128750-59128769 MsG0180003251.01.T01:intron 30.0%
! ATCAATTCATGTTGTTTGGA+TGG - Chr1:59127792-59127811 MsG0180003251.01.T01:intron 30.0%
! CCAAACCAATCAATTTTCTT+TGG + Chr1:59126229-59126248 None:intergenic 30.0%
! CCAAAGAAAATTGATTGGTT+TGG - Chr1:59126226-59126245 MsG0180003251.01.T01:intron 30.0%
! CCAATCAATTTTCTTTGGTT+CGG + Chr1:59126224-59126243 None:intergenic 30.0%
! CGAATAACTTTTTGTCATCT+GGG + Chr1:59126609-59126628 None:intergenic 30.0%
! CTCAAATCGATTCATATGAA+AGG + Chr1:59128305-59128324 None:intergenic 30.0%
! GAGTTGAATATTTCTTTTGG+TGG - Chr1:59128753-59128772 MsG0180003251.01.T01:intron 30.0%
! GATGGCATCTTTTCTTTTTA+TGG - Chr1:59127436-59127455 MsG0180003251.01.T01:intron 30.0%
! TCGAATAACTTTTTGTCATC+TGG + Chr1:59126610-59126629 None:intergenic 30.0%
! TTTTGCATATAGTGTGTGTT+TGG - Chr1:59127667-59127686 MsG0180003251.01.T01:intron 30.0%
!! AAATTGATTGGTTTGGGTAT+TGG - Chr1:59126233-59126252 MsG0180003251.01.T01:intron 30.0%
!! AACACATACTAAGTTTTGTG+AGG + Chr1:59125632-59125651 None:intergenic 30.0%
!! AATTGATTGGTTTGGGTATT+GGG - Chr1:59126234-59126253 MsG0180003251.01.T01:intron 30.0%
!! CTTCAACTGGAATTGATTTT+GGG - Chr1:59128350-59128369 MsG0180003251.01.T01:intron 30.0%
!! GATCTTTTGATTGTGATACA+AGG - Chr1:59128677-59128696 MsG0180003251.01.T01:intron 30.0%
!! GTTGTACTATGATTGATTTG+TGG - Chr1:59128699-59128718 MsG0180003251.01.T01:intron 30.0%
!! TTCAACTGGAATTGATTTTG+GGG - Chr1:59128351-59128370 MsG0180003251.01.T01:intron 30.0%
!!! AATTTTCTTTGGTTCGGTTT+GGG + Chr1:59126218-59126237 None:intergenic 30.0%
!!! CCAAGGTTGTATTTTGTTTT+TGG + Chr1:59128027-59128046 None:intergenic 30.0%
!!! GTTTTGGCTATATGTGAAAA+AGG - Chr1:59125698-59125717 MsG0180003251.01.T01:intron 30.0%
!!! GTTTTTGGAACAATGTCAAA+AGG - Chr1:59127277-59127296 MsG0180003251.01.T01:intron 30.0%
AAACAAGCTTTCTTCACTCT+TGG + Chr1:59128879-59128898 None:intergenic 35.0%
AAAGCTTTACAGAGTAACAG+AGG - Chr1:59127566-59127585 MsG0180003251.01.T01:intron 35.0%
AAAGGTTGTATCTTCTGATG+AGG - Chr1:59128832-59128851 MsG0180003251.01.T01:intron 35.0%
AAGCTTTACAGAGTAACAGA+GGG - Chr1:59127567-59127586 MsG0180003251.01.T01:intron 35.0%
ACAAGTTTGAGCTTGATCAA+AGG - Chr1:59128814-59128833 MsG0180003251.01.T01:intron 35.0%
ATTATAGTAGCCAGTATCTG+GGG + Chr1:59126873-59126892 None:intergenic 35.0%
CACTTGAAAACAACATGCAT+CGG + Chr1:59128527-59128546 None:intergenic 35.0%
CATTAATCGTTGCATGACTT+GGG - Chr1:59126906-59126925 MsG0180003251.01.T01:intron 35.0%
CCGAACCAAAGAAAATTGAT+TGG - Chr1:59126221-59126240 MsG0180003251.01.T01:intron 35.0%
GAATACAGTGATGATTATCC+AGG - Chr1:59127303-59127322 MsG0180003251.01.T01:intron 35.0%
GAGCCTATCTGTACTAAATT+TGG - Chr1:59127331-59127350 MsG0180003251.01.T01:CDS 35.0%
GAGCTATATCCCTTAAAGAT+GGG - Chr1:59126800-59126819 MsG0180003251.01.T01:intron 35.0%
GAGTATCAGTTTACAGAGAA+AGG - Chr1:59127631-59127650 MsG0180003251.01.T01:intron 35.0%
GATTATAGTAGCCAGTATCT+GGG + Chr1:59126874-59126893 None:intergenic 35.0%
GGTAATCATGAAGTAACTTC+CGG - Chr1:59128986-59129005 MsG0180003251.01.T01:intron 35.0%
GTTACTGCGGAAAAAATGAA+TGG - Chr1:59128130-59128149 MsG0180003251.01.T01:intron 35.0%
TCATTAATCGTTGCATGACT+TGG - Chr1:59126905-59126924 MsG0180003251.01.T01:intron 35.0%
TGAGCTATATCCCTTAAAGA+TGG - Chr1:59126799-59126818 MsG0180003251.01.T01:intron 35.0%
TGTAAACTGATACTCAAAGC+AGG + Chr1:59127626-59127645 None:intergenic 35.0%
TTAGGGTATGTTTGGAAGTT+GGG + Chr1:59125967-59125986 None:intergenic 35.0%
TTCACATATAGCCAAAACCA+CGG + Chr1:59125696-59125715 None:intergenic 35.0%
TTGGAACAATGTCAAAAGGA+GGG - Chr1:59127281-59127300 MsG0180003251.01.T01:intron 35.0%
TTTGTAGCGAAGCTACAAAT+AGG + Chr1:59125661-59125680 None:intergenic 35.0%
! ACAGAAATTTTCTCTTGCAC+TGG - Chr1:59126823-59126842 MsG0180003251.01.T01:intron 35.0%
! AGATACTGGCTACTATAATC+CGG - Chr1:59126874-59126893 MsG0180003251.01.T01:intron 35.0%
! ATGAACATTTGAGCCCATTT+TGG + Chr1:59127851-59127870 None:intergenic 35.0%
! GTCATTTAGAAGCTAGTTTC+TGG - Chr1:59128501-59128520 MsG0180003251.01.T01:intron 35.0%
! TATGTTTGGAAGACAGGTTT+TGG - Chr1:59125838-59125857 MsG0180003251.01.T01:intron 35.0%
! TATGTTTGGAAGTTGGGTTT+TGG + Chr1:59125961-59125980 None:intergenic 35.0%
! TCCTTTGAAGAAAACAACCA+TGG + Chr1:59128260-59128279 None:intergenic 35.0%
! TGACTATCTAGTTTGTGGAT+TGG - Chr1:59129066-59129085 MsG0180003251.01.T01:five_prime_UTR 35.0%
! TTTAGGGTATGTTTGGAAGT+TGG + Chr1:59125968-59125987 None:intergenic 35.0%
! TTTGGAACAATGTCAAAAGG+AGG - Chr1:59127280-59127299 MsG0180003251.01.T01:intron 35.0%
!! ACCATGGTTGTTTTCTTCAA+AGG - Chr1:59128256-59128275 MsG0180003251.01.T01:intron 35.0%
!! CTTATTTTCATGAGCTCTCT+AGG - Chr1:59127071-59127090 MsG0180003251.01.T01:CDS 35.0%
!! GCTTCAACTGGAATTGATTT+TGG - Chr1:59128349-59128368 MsG0180003251.01.T01:intron 35.0%
!! TAAGTTTTGTGAGGCTGTTT+GGG + Chr1:59125623-59125642 None:intergenic 35.0%
!! TAATTTACCAAGGGTGTTCA+CGG + Chr1:59126286-59126305 None:intergenic 35.0%
!! TTGGAGAAGATAAGAGTACT+GGG + Chr1:59128166-59128185 None:intergenic 35.0%
!!! ACGACCTTTTGATTTGTCTT+TGG - Chr1:59126480-59126499 MsG0180003251.01.T01:intron 35.0%
!!! CAATTTTCTTTGGTTCGGTT+TGG + Chr1:59126219-59126238 None:intergenic 35.0%
!!! GTTTTTCTGAAACAACTTCC+CGG - Chr1:59128613-59128632 MsG0180003251.01.T01:intron 35.0%
!!! TCTTGATTTTAGGACCTGTT+TGG - Chr1:59125375-59125394 MsG0180003251.01.T01:intron 35.0%
AAGAAATGCTGTCTTGGTTG+AGG - Chr1:59125994-59126013 MsG0180003251.01.T01:intron 40.0%
AATCCTTGGTCTGATATCAC+AGG + Chr1:59129040-59129059 None:intergenic 40.0%
AATTGCTACTACTGCACAGA+TGG - Chr1:59127418-59127437 MsG0180003251.01.T01:intron 40.0%
AGAAATGCTGTCTTGGTTGA+GGG - Chr1:59125995-59126014 MsG0180003251.01.T01:intron 40.0%
AGAACTCTTCAGGGTATGTT+TGG - Chr1:59125824-59125843 MsG0180003251.01.T01:intron 40.0%
AGATCGCTTTCAACTCTAAC+TGG + Chr1:59128662-59128681 None:intergenic 40.0%
ATCACGATGAGAACTCTTCA+GGG - Chr1:59125815-59125834 MsG0180003251.01.T01:intron 40.0%
ATGAAGTGCGTAGAGGAAAA+AGG + Chr1:59124975-59124994 None:intergenic 40.0%
ATTCCTGTGATATCAGACCA+AGG - Chr1:59129034-59129053 MsG0180003251.01.T01:five_prime_UTR 40.0%
CAAACACAAGCCGTTGAATT+GGG + Chr1:59125765-59125784 None:intergenic 40.0%
CGATTGGGAAATTATGACCA+TGG - Chr1:59128240-59128259 MsG0180003251.01.T01:intron 40.0%
CGTGGGTCAAAATGATGTTT+GGG + Chr1:59128403-59128422 None:intergenic 40.0%
GAGAAAAATGAAGTGCGTAG+AGG + Chr1:59124982-59125001 None:intergenic 40.0%
GATCGCTTTCAACTCTAACT+GGG + Chr1:59128661-59128680 None:intergenic 40.0%
GGATTATAGTAGCCAGTATC+TGG + Chr1:59126875-59126894 None:intergenic 40.0%
TAATTTCCCAATCGTGGCAA+CGG + Chr1:59128234-59128253 None:intergenic 40.0%
TCAATACCTCATGCTTCTGT+TGG - Chr1:59125792-59125811 MsG0180003251.01.T01:intron 40.0%
TCCTCATGTAGAGAAGAGAA+GGG - Chr1:59126337-59126356 MsG0180003251.01.T01:intron 40.0%
TCGTGGGTCAAAATGATGTT+TGG + Chr1:59128404-59128423 None:intergenic 40.0%
TGAAGTGCGTAGAGGAAAAA+GGG + Chr1:59124974-59124993 None:intergenic 40.0%
TGCTTGTGATCAGTATGTAG+AGG + Chr1:59126378-59126397 None:intergenic 40.0%
TGGTCATAATTTCCCAATCG+TGG + Chr1:59128240-59128259 None:intergenic 40.0%
TGTGATCAGTATGTAGAGGT+TGG + Chr1:59126374-59126393 None:intergenic 40.0%
TGTTCATGAGCTAGTTGGTT+CGG - Chr1:59126164-59126183 MsG0180003251.01.T01:intron 40.0%
TTTCAGAACCGAAGTTACTG+AGG + Chr1:59126405-59126424 None:intergenic 40.0%
! AGGGTTGAAATATTGGGTTG+AGG + Chr1:59124955-59124974 None:intergenic 40.0%
! CAGCATGACTATCTAGTTTG+TGG - Chr1:59129061-59129080 MsG0180003251.01.T01:five_prime_UTR 40.0%
! GTTGGAGAAGATAAGAGTAC+TGG + Chr1:59128167-59128186 None:intergenic 40.0%
!! AATTTACCAAGGGTGTTCAC+GGG + Chr1:59126285-59126304 None:intergenic 40.0%
!! ATAGTGTGTGTTTGGTTCTG+CGG - Chr1:59127675-59127694 MsG0180003251.01.T01:intron 40.0%
!! ATTGGTTTGGGTATTGGGTT+TGG - Chr1:59126239-59126258 MsG0180003251.01.T01:intron 40.0%
!! CTAAGTTTTGTGAGGCTGTT+TGG + Chr1:59125624-59125643 None:intergenic 40.0%
!! GCTGTTTTCATAAGCTGTCT+TGG - Chr1:59125531-59125550 MsG0180003251.01.T01:intron 40.0%
!! GTAGTTTTGCACTGCTTCTA+GGG - Chr1:59125148-59125167 MsG0180003251.01.T01:CDS 40.0%
!!! AAGACAGGTTTTGGAGGAAA+AGG - Chr1:59125847-59125866 MsG0180003251.01.T01:intron 40.0%
!!! AGACAGGTTTTGGAGGAAAA+GGG - Chr1:59125848-59125867 MsG0180003251.01.T01:intron 40.0%
!!! CGATGCATGTTGTTTTCAAG+TGG - Chr1:59128525-59128544 MsG0180003251.01.T01:intron 40.0%
AAACGTGTTCGCATGGAACT+AGG + Chr1:59127261-59127280 None:intergenic 45.0%
AACAAGACTATGCCCCTGTT+TGG + Chr1:59128476-59128495 None:intergenic 45.0%
AACGTGTTCGCATGGAACTA+GGG + Chr1:59127260-59127279 None:intergenic 45.0%
ACAAACGTGAATTAGTGCCG+TGG - Chr1:59125676-59125695 MsG0180003251.01.T01:intron 45.0%
ACCCTTCTCTTCTCTACATG+AGG + Chr1:59126341-59126360 None:intergenic 45.0%
ACGATTTCTCGTACGACAAG+TGG + Chr1:59125130-59125149 None:intergenic 45.0%
AGTGAGCTGAACTGCTACAA+TGG - Chr1:59126540-59126559 MsG0180003251.01.T01:intron 45.0%
CAACCTGTTCATGAGCTAGT+TGG - Chr1:59126159-59126178 MsG0180003251.01.T01:intron 45.0%
CAACTAGCTCATGAACAGGT+TGG + Chr1:59126161-59126180 None:intergenic 45.0%
CCAAACACAAGCCGTTGAAT+TGG + Chr1:59125766-59125785 None:intergenic 45.0%
CCAATTCAACGGCTTGTGTT+TGG - Chr1:59125763-59125782 MsG0180003251.01.T01:intron 45.0%
CTCCTCATGTAGAGAAGAGA+AGG - Chr1:59126336-59126355 MsG0180003251.01.T01:intron 45.0%
GAACCAACTAGCTCATGAAC+AGG + Chr1:59126165-59126184 None:intergenic 45.0%
GATAGTCATGCTGCAATCCT+TGG + Chr1:59129054-59129073 None:intergenic 45.0%
GATCACGATGAGAACTCTTC+AGG - Chr1:59125814-59125833 MsG0180003251.01.T01:intron 45.0%
GTAGAACGCAGAACCAAACA+GGG - Chr1:59128460-59128479 MsG0180003251.01.T01:intron 45.0%
GTTCCAAAAACGTGTTCGCA+TGG + Chr1:59127268-59127287 None:intergenic 45.0%
GTTCCAACACTCAATCCTGA+TGG - Chr1:59126845-59126864 MsG0180003251.01.T01:intron 45.0%
TACAGAGTAACAGAGGGTTC+AGG - Chr1:59127573-59127592 MsG0180003251.01.T01:intron 45.0%
TACAGATAGGCTCAAACACC+TGG + Chr1:59127324-59127343 None:intergenic 45.0%
TAGAACGCAGAACCAAACAG+GGG - Chr1:59128461-59128480 MsG0180003251.01.T01:intron 45.0%
TCAGGGTATGTTTGGAAGAC+AGG - Chr1:59125832-59125851 MsG0180003251.01.T01:intron 45.0%
TGTAGAACGCAGAACCAAAC+AGG - Chr1:59128459-59128478 MsG0180003251.01.T01:intron 45.0%
TTTGAGTAGACGCAGAAGCT+AGG - Chr1:59128319-59128338 MsG0180003251.01.T01:intron 45.0%
! CAATTGATTGCCGATTACCC+TGG + Chr1:59127518-59127537 None:intergenic 45.0%
! CTAGGAAAACTGGCTTCAAC+TGG - Chr1:59128337-59128356 MsG0180003251.01.T01:intron 45.0%
! GTGAATTAGTGCCGTGGTTT+TGG - Chr1:59125682-59125701 MsG0180003251.01.T01:intron 45.0%
! GTTCCATGCGAACACGTTTT+TGG - Chr1:59127262-59127281 MsG0180003251.01.T01:intron 45.0%
!! AGGGGCATAGTCTTGTTGAT+TGG - Chr1:59128479-59128498 MsG0180003251.01.T01:intron 45.0%
!! CGTAGTTTTGCACTGCTTCT+AGG - Chr1:59125147-59125166 MsG0180003251.01.T01:CDS 45.0%
!! GTTTGGAAGACAGGTTTTGG+AGG - Chr1:59125841-59125860 MsG0180003251.01.T01:intron 45.0%
!! GTTTGGAAGTTGGGTTTTGG+AGG + Chr1:59125958-59125977 None:intergenic 45.0%
!! TATAGTAATTAAAAATTAAT+CGG - Chr1:59127951-59127970 MsG0180003251.01.T01:intron 5.0%
!!! TTTTTTGTAAATTTTTTAAT+TGG - Chr1:59125204-59125223 MsG0180003251.01.T01:intron 5.0%
AGAGTAACAGAGGGTTCAGG+AGG - Chr1:59127576-59127595 MsG0180003251.01.T01:intron 50.0%
AGCACGCACCTCAGTAACTT+CGG - Chr1:59126394-59126413 MsG0180003251.01.T01:CDS 50.0%
CACCAAGAGAAGAGGAGGAA+CGG + Chr1:59124920-59124939 None:intergenic 50.0%
GACGCAGAAGCTAGGAAAAC+TGG - Chr1:59128327-59128346 MsG0180003251.01.T01:intron 50.0%
GAGCTGAACTGCTACAATGG+TGG - Chr1:59126543-59126562 MsG0180003251.01.T01:intron 50.0%
GAGTAACAGAGGGTTCAGGA+GGG - Chr1:59127577-59127596 MsG0180003251.01.T01:intron 50.0%
GTGCCATCAGGATTGAGTGT+TGG + Chr1:59126851-59126870 None:intergenic 50.0%
GTGTGACTCACCAAGAGAAG+AGG + Chr1:59124928-59124947 None:intergenic 50.0%
TGACTCACCAAGAGAAGAGG+AGG + Chr1:59124925-59124944 None:intergenic 50.0%
! AGCTCATGAACAGGTTGGAC+AGG + Chr1:59126156-59126175 None:intergenic 50.0%
! CGTGATCCAACAGAAGCATG+AGG + Chr1:59125801-59125820 None:intergenic 50.0%
! GCTCATGAACAGGTTGGACA+GGG + Chr1:59126155-59126174 None:intergenic 50.0%
! GGGCTTCTGATGATGCTGAA+GGG - Chr1:59128774-59128793 MsG0180003251.01.T01:intron 50.0%
! TGCATGACTTGGGATCTCTC+TGG - Chr1:59126916-59126935 MsG0180003251.01.T01:intron 50.0%
! TGGGCTTCTGATGATGCTGA+AGG - Chr1:59128773-59128792 MsG0180003251.01.T01:intron 50.0%
AAACGGCCGTTGCCACGATT+GGG - Chr1:59128225-59128244 MsG0180003251.01.T01:intron 55.0%
ACCACGCAAACGTGCGTTTG+AGG - Chr1:59128435-59128454 MsG0180003251.01.T01:intron 55.0%
CAAGAGAAGAGGAGGAACGG+TGG + Chr1:59124917-59124936 None:intergenic 55.0%
CACCGTTCCTCCTCTTCTCT+TGG - Chr1:59124915-59124934 MsG0180003251.01.T01:three_prime_UTR 55.0%
CACGTTTGCGTGGTCAAACG+CGG + Chr1:59128429-59128448 None:intergenic 55.0%
CGCATGGAACTAGGGACTGA+GGG + Chr1:59127252-59127271 None:intergenic 55.0%
GTGGTCAAACGCGGTTTCGT+GGG + Chr1:59128420-59128439 None:intergenic 55.0%
TCGCATGGAACTAGGGACTG+AGG + Chr1:59127253-59127272 None:intergenic 55.0%
!! ACCAAGGGTGTTCACGGGTT+GGG + Chr1:59126280-59126299 None:intergenic 55.0%
!! GTGTGTGTTTGGTTCTGCGG+TGG - Chr1:59127678-59127697 MsG0180003251.01.T01:intron 55.0%
!! TACCAAGGGTGTTCACGGGT+TGG + Chr1:59126281-59126300 None:intergenic 55.0%
!! TCCCACCTCTCACTGATTCC+CGG - Chr1:59124873-59124892 MsG0180003251.01.T01:three_prime_UTR 55.0%
ACCCAACCCGTGAACACCCT+TGG - Chr1:59126276-59126295 MsG0180003251.01.T01:intron 60.0%
CCTGATGGCACCCCAGATAC+TGG - Chr1:59126860-59126879 MsG0180003251.01.T01:intron 60.0%
CGTGGTCAAACGCGGTTTCG+TGG + Chr1:59128421-59128440 None:intergenic 60.0%
GAAACGGCCGTTGCCACGAT+TGG - Chr1:59128224-59128243 MsG0180003251.01.T01:intron 60.0%
GAGAAGAGGAGGAACGGTGG+TGG + Chr1:59124914-59124933 None:intergenic 60.0%
GCCGGGAATCAGTGAGAGGT+GGG + Chr1:59124877-59124896 None:intergenic 60.0%
GCCTCAAACGCACGTTTGCG+TGG + Chr1:59128439-59128458 None:intergenic 60.0%
! CCAGTATCTGGGGTGCCATC+AGG + Chr1:59126863-59126882 None:intergenic 60.0%
!! AGGGTGTTCACGGGTTGGGT+TGG + Chr1:59126276-59126295 None:intergenic 60.0%
!! GAGGAACGGTGGTGGATAGG+AGG + Chr1:59124906-59124925 None:intergenic 60.0%
!! GAGGAGGAACGGTGGTGGAT+AGG + Chr1:59124909-59124928 None:intergenic 60.0%
!! GGGTGTTCACGGGTTGGGTT+GGG + Chr1:59126275-59126294 None:intergenic 60.0%
CGCCGGGAATCAGTGAGAGG+TGG + Chr1:59124878-59124897 None:intergenic 65.0%
CTGCGCCGGGAATCAGTGAG+AGG + Chr1:59124881-59124900 None:intergenic 65.0%
GTGGATAGGAGGAGCTGCGC+CGG + Chr1:59124895-59124914 None:intergenic 65.0%
TGGATAGGAGGAGCTGCGCC+GGG + Chr1:59124894-59124913 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
Chr1 gene 59124832 59129120 59124832 ID=MsG0180003251.01;Name=MsG0180003251.01
Chr1 mRNA 59124832 59129120 59124832 ID=MsG0180003251.01.T01;Parent=MsG0180003251.01;Name=MsG0180003251.01.T01;_AED=0.48;_eAED=0.48;_QI=122|1|1|1|0.75|0.6|5|257|145
Chr1 exon 59124832 59125200 59124832 ID=MsG0180003251.01.T01:exon:23105;Parent=MsG0180003251.01.T01
Chr1 exon 59126342 59126433 59126342 ID=MsG0180003251.01.T01:exon:23104;Parent=MsG0180003251.01.T01
Chr1 exon 59127057 59127214 59127057 ID=MsG0180003251.01.T01:exon:23103;Parent=MsG0180003251.01.T01
Chr1 exon 59127318 59127410 59127318 ID=MsG0180003251.01.T01:exon:23102;Parent=MsG0180003251.01.T01
Chr1 exon 59129016 59129120 59129016 ID=MsG0180003251.01.T01:exon:23101;Parent=MsG0180003251.01.T01
Chr1 five_prime_UTR 59129016 59129120 59129016 ID=MsG0180003251.01.T01:five_prime_utr;Parent=MsG0180003251.01.T01
Chr1 five_prime_UTR 59127394 59127410 59127394 ID=MsG0180003251.01.T01:five_prime_utr;Parent=MsG0180003251.01.T01
Chr1 CDS 59127318 59127393 59127318 ID=MsG0180003251.01.T01:cds;Parent=MsG0180003251.01.T01
Chr1 CDS 59127057 59127214 59127057 ID=MsG0180003251.01.T01:cds;Parent=MsG0180003251.01.T01
Chr1 CDS 59126342 59126433 59126342 ID=MsG0180003251.01.T01:cds;Parent=MsG0180003251.01.T01
Chr1 CDS 59125089 59125200 59125089 ID=MsG0180003251.01.T01:cds;Parent=MsG0180003251.01.T01
Chr1 three_prime_UTR 59124832 59125088 59124832 ID=MsG0180003251.01.T01:three_prime_utr;Parent=MsG0180003251.01.T01
Gene Sequence

>MsG0180003251.01.T01

ATGGTGGAGTCACTTCTTGAAGATATCTTTAGGGTTGAGAAAGTCAACCCAGATGACAAAAAGTTATTCGAAAAAGTGACACGCATTGAAGCAAGAAGTGAAAAGTTTGACATGTTTATGCAACTAGACATAAATTCTGAGCTATATCCCTTAAAGATGGGACAGAAATTTTCTCTTGCACTGGTTCCAACACTCAATCCTGATGGCACCCCAGATACTGGCTACTATAATCCGGGTAATCGGCAATCAATTGCTGATAACTATGAATATGTCATGTATGGAAAGCTTTACAGAGTAACAGAGGGTTCAGGAGGGCGTGAAAAAGCGGAGTTGAATATTTCTTTTGGTGGGCTTCTGATGATGCTGAAGGGAGATCACTCTCATTTCAACAAGTTTGAGCTTGATCAAAGGTTGTATCTTCTGATGAGGAAAGTCTGA
Protein sequence

>MsG0180003251.01.T01

MVESLLEDIFRVEKVNPDDKKLFEKVTRIEARSEKFDMFMQLDINSELYPLKMGQKFSLALVPTLNPDGTPDTGYYNPGNRQSIADNYEYVMYGKLYRVTEGSGGREKAELNISFGGLLMMLKGDHSHFNKFELDQRLYLLMRKV*