AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0180003315.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180003315.01.T01 MTR_1g058380 92.484 306 11 1 1 306 1 294 0.0 582
MsG0180003315.01.T01 MTR_2g022560 57.255 255 93 3 53 306 95 334 9.34e-101 303
MsG0180003315.01.T01 MTR_7g094750 53.755 253 101 3 55 306 91 328 4.32e-98 292
MsG0180003315.01.T01 MTR_7g094750 53.333 255 103 3 53 306 89 328 1.56e-96 292
MsG0180003315.01.T01 MTR_7g109550 44.488 254 121 5 55 306 137 372 3.70e-71 228
MsG0180003315.01.T01 MTR_3g090120 40.392 255 138 3 53 305 127 369 1.04e-66 216
MsG0180003315.01.T01 MTR_4g126950 41.313 259 133 5 50 306 104 345 1.13e-65 213
MsG0180003315.01.T01 MTR_5g033800 38.039 255 140 4 53 306 143 380 3.42e-60 197
MsG0180003315.01.T01 MTR_8g093530 35.762 302 174 6 5 305 44 326 5.50e-60 197
MsG0180003315.01.T01 MTR_5g033800 38.039 255 140 4 53 306 143 380 1.04e-58 196
MsG0180003315.01.T01 MTR_5g059400 38.340 253 139 4 55 306 175 411 1.32e-58 196
MsG0180003315.01.T01 MTR_1g116130 38.340 253 139 4 55 306 175 411 1.32e-58 196
MsG0180003315.01.T01 MTR_7g093450 38.889 252 137 4 55 305 248 483 9.75e-57 193
MsG0180003315.01.T01 MTR_0136s0010 39.286 252 136 4 55 305 170 405 2.65e-56 190
MsG0180003315.01.T01 MTR_4g121560 34.296 277 161 4 37 306 90 352 1.93e-55 186
MsG0180003315.01.T01 MTR_8g069790 37.255 255 140 4 53 305 176 412 1.85e-54 185
MsG0180003315.01.T01 MTR_1g050448 33.981 309 171 8 1 307 1 278 4.73e-54 181
MsG0180003315.01.T01 MTR_2g022580 60.800 125 49 0 117 241 2 126 3.19e-51 165
MsG0180003315.01.T01 MTR_1g112210 34.902 255 139 5 53 307 57 284 1.10e-48 167
MsG0180003315.01.T01 MTR_5g071350 35.433 254 137 6 54 307 57 283 3.11e-48 166
MsG0180003315.01.T01 MTR_5g083260 34.848 264 137 6 53 308 81 317 2.50e-47 164
MsG0180003315.01.T01 MTR_2g015720 33.591 259 143 6 47 305 34 263 3.25e-47 162
MsG0180003315.01.T01 MTR_2g091175 35.968 253 132 6 55 306 50 273 3.55e-47 162
MsG0180003315.01.T01 MTR_1g112210 33.725 255 129 5 53 307 57 271 1.93e-46 160
MsG0180003315.01.T01 MTR_3g070900 36.047 258 144 5 50 306 101 338 3.70e-46 162
MsG0180003315.01.T01 MTR_3g464570 35.458 251 135 6 54 304 42 265 1.09e-45 159
MsG0180003315.01.T01 MTR_4g016940 33.333 264 141 6 53 308 84 320 1.53e-44 157
MsG0180003315.01.T01 MTR_8g463790 34.902 255 137 8 53 304 69 297 1.79e-44 157
MsG0180003315.01.T01 MTR_4g079700 33.456 272 151 7 36 306 28 270 3.86e-44 154
MsG0180003315.01.T01 MTR_3g071650 34.510 255 146 4 55 308 89 323 4.21e-44 156
MsG0180003315.01.T01 MTR_2g073500 34.766 256 139 7 53 304 27 258 7.58e-44 153
MsG0180003315.01.T01 MTR_2g073510 34.902 255 135 6 53 305 31 256 7.78e-43 150
MsG0180003315.01.T01 MTR_4g088030 32.000 275 154 8 31 304 12 254 8.02e-43 150
MsG0180003315.01.T01 MTR_4g087730 32.090 268 150 8 40 306 32 268 1.25e-42 150
MsG0180003315.01.T01 MTR_3g049330 35.156 256 128 8 54 304 30 252 1.28e-41 147
MsG0180003315.01.T01 MTR_5g034810 33.591 259 140 8 47 304 27 254 1.43e-41 147
MsG0180003315.01.T01 MTR_5g082880 32.567 261 152 6 50 306 160 400 2.74e-40 147
MsG0180003315.01.T01 MTR_7g068920 32.157 255 152 5 53 306 78 312 1.10e-39 144
MsG0180003315.01.T01 MTR_1g111010 32.411 253 142 7 53 304 30 254 1.14e-39 142
MsG0180003315.01.T01 MTR_7g070060 32.331 266 156 6 44 306 53 297 2.10e-39 140
MsG0180003315.01.T01 MTR_4g014440 31.679 262 153 8 49 306 56 295 2.12e-39 143
MsG0180003315.01.T01 MTR_3g021060 33.858 254 138 8 53 305 44 268 3.20e-39 141
MsG0180003315.01.T01 MTR_4g014430 30.798 263 156 7 44 304 97 335 4.86e-39 142
MsG0180003315.01.T01 MTR_7g070060 31.698 265 159 5 44 306 53 297 5.19e-38 139
MsG0180003315.01.T01 MTR_3g035830 31.890 254 143 7 53 305 29 253 1.26e-36 134
MsG0180003315.01.T01 MTR_3g021090 32.016 253 142 8 53 304 29 252 6.05e-35 131
MsG0180003315.01.T01 MTR_5g035090 34.071 226 132 9 55 279 83 292 1.01e-28 114
MsG0180003315.01.T01 MTR_3g464570 33.880 183 96 5 122 304 4 161 2.94e-25 102
MsG0180003315.01.T01 MTR_8g027900 29.804 255 156 9 55 306 211 445 3.65e-25 105
MsG0180003315.01.T01 MTR_5g035080 29.658 263 165 9 45 305 65 309 8.74e-25 103
MsG0180003315.01.T01 MTR_6g016880 28.309 272 151 9 49 306 72 313 1.92e-24 102
MsG0180003315.01.T01 MTR_7g019120 27.018 285 163 10 35 306 62 314 9.76e-24 100
MsG0180003315.01.T01 MTR_7g033230 30.855 269 158 12 45 306 253 500 1.41e-23 101
MsG0180003315.01.T01 MTR_5g035150 28.516 256 164 8 53 306 83 321 2.61e-23 99.8
MsG0180003315.01.T01 MTR_7g033230 31.086 267 156 12 47 306 325 570 2.93e-23 100
MsG0180003315.01.T01 MTR_3g109840 26.885 305 197 11 5 306 25 306 5.48e-22 95.9
MsG0180003315.01.T01 MTR_2g016060 26.877 253 160 7 60 306 60 293 1.03e-21 94.7
MsG0180003315.01.T01 MTR_6g016875 29.412 272 143 10 53 306 62 302 1.24e-21 94.7
MsG0180003315.01.T01 MTR_8g093870 28.179 291 170 13 29 305 114 379 3.82e-21 93.6
MsG0180003315.01.T01 MTR_8g104970 29.213 267 161 11 47 306 111 356 3.84e-21 93.6
MsG0180003315.01.T01 MTR_4g125060 28.352 261 167 9 53 305 130 378 7.77e-21 92.8
MsG0180003315.01.T01 MTR_4g125060 28.352 261 167 9 53 305 198 446 1.72e-20 92.0
MsG0180003315.01.T01 MTR_4g125060 28.352 261 167 9 53 305 169 417 2.45e-20 91.7
MsG0180003315.01.T01 MTR_6g061710 27.985 268 167 8 43 306 155 400 4.15e-20 90.9
MsG0180003315.01.T01 MTR_7g019140 40.000 115 60 3 35 147 62 169 5.04e-20 85.9
MsG0180003315.01.T01 MTR_4g092830 29.876 241 145 7 55 281 48 278 5.12e-20 90.1
MsG0180003315.01.T01 MTR_1g076690 28.929 280 150 12 43 306 210 456 9.59e-20 89.7
MsG0180003315.01.T01 MTR_6g016240 31.606 193 111 9 53 233 69 252 4.28e-19 87.4
MsG0180003315.01.T01 MTR_7g070060 27.136 199 126 3 109 306 15 195 3.39e-18 83.6
MsG0180003315.01.T01 MTR_5g035120 39.604 101 57 2 53 152 73 170 4.76e-17 77.8
MsG0180003315.01.T01 MTR_6g007777 24.830 294 151 13 39 306 105 354 9.59e-17 80.9
MsG0180003315.01.T01 MTR_5g035070 29.515 227 145 7 54 279 86 298 2.46e-16 79.3
MsG0180003315.01.T01 MTR_8g099300 25.098 255 169 9 55 306 74 309 1.16e-15 77.4
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180003315.01.T01 AT3G11570 55.449 312 114 5 1 306 27 319 2.73e-116 342
MsG0180003315.01.T01 AT5G06230 60.078 258 87 2 53 306 60 305 1.94e-112 328
MsG0180003315.01.T01 AT5G06230 57.194 278 98 3 33 306 4 264 2.28e-111 327
MsG0180003315.01.T01 AT5G06230 57.194 278 98 3 33 306 45 305 4.50e-111 328
MsG0180003315.01.T01 AT5G19160 50.391 256 111 3 54 308 98 338 5.36e-95 285
MsG0180003315.01.T01 AT5G19160 50.394 254 110 3 54 306 98 336 1.32e-92 283
MsG0180003315.01.T01 AT3G06080 51.562 256 108 3 54 308 102 342 3.62e-91 275
MsG0180003315.01.T01 AT3G06080 51.575 254 107 3 54 306 102 340 1.39e-89 275
MsG0180003315.01.T01 AT3G62390 44.186 258 126 5 52 308 135 375 2.72e-72 229
MsG0180003315.01.T01 AT3G62390 44.314 255 124 5 52 305 135 372 3.02e-70 225
MsG0180003315.01.T01 AT5G20590 42.188 256 132 4 52 306 138 378 1.65e-68 218
MsG0180003315.01.T01 AT5G20590 42.188 256 132 4 52 306 138 378 4.69e-67 218
MsG0180003315.01.T01 AT1G48880 40.873 252 133 3 55 305 115 351 7.50e-64 208
MsG0180003315.01.T01 AT3G12060 40.000 255 136 4 52 305 193 431 9.72e-60 200
MsG0180003315.01.T01 AT1G60790 38.760 258 137 5 53 306 188 428 2.89e-59 198
MsG0180003315.01.T01 AT5G06700 38.182 275 145 5 36 305 236 490 8.77e-58 196
MsG0180003315.01.T01 AT3G55990 37.548 261 142 5 49 308 134 374 3.09e-50 173
MsG0180003315.01.T01 AT2G30010 36.426 291 152 8 23 305 17 282 3.59e-50 171
MsG0180003315.01.T01 AT2G30010 36.426 291 152 8 23 305 60 325 6.32e-50 171
MsG0180003315.01.T01 AT2G40150 37.132 272 150 5 36 306 52 303 4.90e-49 169
MsG0180003315.01.T01 AT3G54260 35.484 248 133 4 55 302 51 271 7.34e-48 164
MsG0180003315.01.T01 AT5G49340 31.618 272 159 7 43 307 88 339 7.52e-48 166
MsG0180003315.01.T01 AT2G40160 34.307 274 156 10 38 306 58 312 1.21e-47 162
MsG0180003315.01.T01 AT2G40160 34.307 274 156 10 38 306 58 312 3.15e-46 161
MsG0180003315.01.T01 AT2G40160 34.307 274 156 10 38 306 58 312 4.84e-46 161
MsG0180003315.01.T01 AT2G42570 32.721 272 151 7 37 305 27 269 4.96e-45 157
MsG0180003315.01.T01 AT3G11030 33.725 255 148 4 53 306 104 338 5.13e-45 159
MsG0180003315.01.T01 AT1G73140 35.273 275 145 9 35 304 47 293 1.68e-44 157
MsG0180003315.01.T01 AT5G58600 34.524 252 137 7 54 304 65 289 1.03e-43 151
MsG0180003315.01.T01 AT5G58600 34.387 253 138 7 53 304 64 289 1.42e-42 151
MsG0180003315.01.T01 AT2G40320 32.197 264 144 7 53 308 79 315 1.53e-42 152
MsG0180003315.01.T01 AT2G30900 33.217 286 144 10 32 306 19 268 8.41e-42 148
MsG0180003315.01.T01 AT2G34070 32.677 254 143 7 53 305 63 289 3.59e-41 147
MsG0180003315.01.T01 AT5G01360 30.588 255 156 4 53 306 34 268 4.22e-41 147
MsG0180003315.01.T01 AT1G78710 33.068 251 138 7 55 304 38 259 4.61e-41 146
MsG0180003315.01.T01 AT1G29050 31.418 261 150 7 46 305 52 284 8.15e-41 146
MsG0180003315.01.T01 AT5G01360 30.588 255 156 4 53 306 90 324 8.25e-41 147
MsG0180003315.01.T01 AT2G38320 34.252 254 145 7 55 306 59 292 1.05e-40 146
MsG0180003315.01.T01 AT2G30900 33.704 270 133 9 39 298 27 260 1.41e-40 143
MsG0180003315.01.T01 AT2G31110 31.746 252 142 7 55 305 45 267 1.29e-39 142
MsG0180003315.01.T01 AT2G31110 30.894 246 140 7 55 299 45 261 4.94e-37 133
MsG0180003315.01.T01 AT3G14850 33.463 257 141 8 53 308 32 259 9.40e-36 132
MsG0180003315.01.T01 AT5G01620 28.968 252 158 4 53 304 108 338 2.00e-35 133
MsG0180003315.01.T01 AT5G01620 28.968 252 158 4 53 304 108 338 2.00e-35 133
MsG0180003315.01.T01 AT5G01620 28.968 252 158 4 53 304 116 346 2.71e-35 133
MsG0180003315.01.T01 AT2G38320 34.081 223 128 5 85 306 5 209 1.39e-34 128
MsG0180003315.01.T01 AT1G78710 32.338 201 118 6 55 254 31 214 2.22e-29 114
MsG0180003315.01.T01 AT1G70230 31.225 253 150 9 55 306 79 308 1.18e-27 111
MsG0180003315.01.T01 AT4G01080 27.597 308 190 11 2 306 43 320 1.23e-25 106
MsG0180003315.01.T01 AT5G15890 28.986 276 154 9 41 306 173 416 1.51e-25 106
MsG0180003315.01.T01 AT5G64470 28.846 260 155 8 55 305 55 293 3.14e-25 103
MsG0180003315.01.T01 AT5G64470 28.846 260 155 8 55 305 55 293 3.97e-25 103
MsG0180003315.01.T01 AT5G64470 28.846 260 155 8 55 305 55 293 1.27e-24 103
MsG0180003315.01.T01 AT2G37720 31.655 278 163 12 41 306 128 390 1.37e-24 103
MsG0180003315.01.T01 AT5G64470 28.846 260 155 8 55 305 55 293 1.38e-24 102
MsG0180003315.01.T01 AT1G01430 30.196 255 154 10 55 306 99 332 3.34e-24 102
MsG0180003315.01.T01 AT2G14530 28.358 268 161 10 51 306 57 305 2.05e-23 99.8
MsG0180003315.01.T01 AT5G64020 31.461 267 151 11 54 306 56 304 2.17e-22 96.7
MsG0180003315.01.T01 AT5G51640 47.826 92 44 2 55 145 138 226 9.90e-21 92.8
MsG0180003315.01.T01 AT2G34070 30.726 179 100 5 127 305 1 155 1.99e-20 89.0
MsG0180003315.01.T01 AT4G25360 41.284 109 59 3 39 145 158 263 2.17e-20 91.7
MsG0180003315.01.T01 AT4G25360 41.284 109 59 3 39 145 158 263 2.17e-20 91.7
MsG0180003315.01.T01 AT4G11090 28.352 261 164 10 50 306 74 315 2.26e-20 91.3
MsG0180003315.01.T01 AT4G23790 28.852 305 186 13 12 306 33 316 9.51e-20 89.4
MsG0180003315.01.T01 AT5G20680 31.222 221 137 9 26 237 184 398 9.53e-20 89.7
MsG0180003315.01.T01 AT5G20680 31.222 221 137 9 26 237 184 398 9.53e-20 89.7
MsG0180003315.01.T01 AT5G20680 31.222 221 137 9 26 237 184 398 9.53e-20 89.7
MsG0180003315.01.T01 AT5G20680 31.222 221 137 9 26 237 184 398 9.53e-20 89.7
MsG0180003315.01.T01 AT5G20680 31.222 221 137 9 26 237 90 304 1.02e-19 89.4
MsG0180003315.01.T01 AT5G20680 31.222 221 137 9 26 237 166 380 1.07e-19 89.7
MsG0180003315.01.T01 AT5G15900 25.573 262 165 11 53 306 84 323 4.05e-18 84.7
MsG0180003315.01.T01 AT5G15900 25.573 262 165 11 53 306 65 304 4.22e-18 84.7
MsG0180003315.01.T01 AT3G02440 24.830 294 183 10 28 305 97 368 3.29e-17 82.4
MsG0180003315.01.T01 AT3G02440 24.746 295 184 10 28 306 89 361 3.38e-17 82.0
MsG0180003315.01.T01 AT3G28150 44.086 93 48 2 55 146 70 159 4.55e-16 78.6
MsG0180003315.01.T01 AT3G14850 30.220 182 101 6 127 308 1 156 2.09e-14 72.0
MsG0180003315.01.T01 AT2G31110 30.827 133 73 4 173 305 6 119 4.30e-11 62.0

Find 60 sgRNAs with CRISPR-Local

Find 138 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTATCCCTTGTGTTTGCTTA+TGG 0.207550 1:-60489872 None:intergenic
TACATTGGTATTCTAATAAC+TGG 0.269659 1:+60490022 MsG0180003315.01.T01:CDS
TATCCCTTGTGTTTGCTTAT+GGG 0.281303 1:-60489871 None:intergenic
GCACCTTTCTTATCTATTAT+AGG 0.282563 1:+60489943 MsG0180003315.01.T01:CDS
CATTTACCTTCCCACCTTCC+TGG 0.294788 1:-60490344 MsG0180003316.01.T01:three_prime_UTR
TTTCCAAAGGTCAATGGGTT+TGG 0.302027 1:+60488331 MsG0180003315.01.T01:CDS
ACATGACTGTAGAATATTAT+AGG 0.311467 1:+60489920 MsG0180003315.01.T01:CDS
CCTTTCTTGATCCCGGATTC+CGG 0.316435 1:+60488397 MsG0180003315.01.T01:CDS
ACGACGAAGGTTATCGTAAA+TGG 0.317486 1:+60488439 MsG0180003315.01.T01:CDS
TTCCAAAGGTCAATGGGTTT+GGG 0.343647 1:+60488332 MsG0180003315.01.T01:CDS
GGATTCCGGTGCCGGCAAAA+TGG 0.347438 1:+60488411 MsG0180003315.01.T01:CDS
CTTGGAAGGTCACAATCATT+AGG 0.374851 1:-60488471 None:intergenic
GCTATGCCCCTGTTCATTAC+AGG 0.375757 1:+60490469 MsG0180003315.01.T01:CDS
TTGCAGGGGTATCTATTTCC+AGG 0.376397 1:+60490326 MsG0180003315.01.T01:CDS
GTCCCAAACCCATTGACCTT+TGG 0.383439 1:-60488334 None:intergenic
TATACTTGCCTGTAATGAAC+AGG 0.389013 1:-60490477 MsG0180003316.01.T01:intron
TTGATCCCGGATTCCGGTGC+CGG 0.395661 1:+60488403 MsG0180003315.01.T01:CDS
CATGACTGTAGAATATTATA+GGG 0.397772 1:+60489921 MsG0180003315.01.T01:CDS
AATGGACGCATTGTGTTTGC+AGG 0.402906 1:+60489754 MsG0180003315.01.T01:CDS
TACTTTGCATGTTGTCAAAA+GGG 0.411641 1:+60489809 MsG0180003315.01.T01:CDS
TTACTTTGCATGTTGTCAAA+AGG 0.415390 1:+60489808 MsG0180003315.01.T01:CDS
CCTTACAGACATGGAAATCA+TGG 0.428443 1:+60490400 MsG0180003315.01.T01:CDS
AATTGTCCCTTTCTTGATCC+CGG 0.459896 1:+60488390 MsG0180003315.01.T01:CDS
CCATGATTTCCATGTCTGTA+AGG 0.482202 1:-60490400 MsG0180003316.01.T01:three_prime_UTR
ATACTTAGTGATGAAATTTG+AGG 0.487775 1:+60489891 MsG0180003315.01.T01:CDS
CCGGAATCCGGGATCAAGAA+AGG 0.488839 1:-60488397 None:intergenic
AAGAAGACGAAACTCCTCAT+AGG 0.494604 1:-60490441 MsG0180003316.01.T01:three_prime_UTR
CTAATGATTGTGACCTTCCA+AGG 0.517319 1:+60488472 MsG0180003315.01.T01:CDS
TTCAGTGGGCAGAAACCAAT+GGG 0.529626 1:+60489780 MsG0180003315.01.T01:CDS
TGCAAAGTAATGACTCCCAT+TGG 0.533961 1:-60489795 None:intergenic
GGTATCTATTTCCAGGAAGG+TGG 0.535569 1:+60490333 MsG0180003315.01.T01:CDS
ATTCAGTGGGCAGAAACCAA+TGG 0.546878 1:+60489779 MsG0180003315.01.T01:CDS
CGGAATCCGGGATCAAGAAA+GGG 0.564222 1:-60488396 None:intergenic
ACTTTGCATGTTGTCAAAAG+GGG 0.566215 1:+60489810 MsG0180003315.01.T01:CDS
GTATCTATTTCCAGGAAGGT+GGG 0.572325 1:+60490334 MsG0180003315.01.T01:CDS
GTCCATTGCGACTCCTCTCT+AGG 0.578072 1:-60489738 None:intergenic
TGACCTATAATAGATAAGAA+AGG 0.579713 1:-60489946 None:intergenic
TCCGGTGCCGGCAAAATGGA+AGG 0.580221 1:+60488415 MsG0180003315.01.T01:CDS
TTGGTATTCTAATAACTGGA+AGG 0.580759 1:+60490026 MsG0180003315.01.T01:CDS
CTCCTAGAGAGGAGTCGCAA+TGG 0.581234 1:+60489736 MsG0180003315.01.T01:CDS
TGAGTAATCTAAATCCTATG+AGG 0.582191 1:+60490427 MsG0180003315.01.T01:CDS
ACTCCTCTCTAGGAGGTCAC+TGG 0.585040 1:-60489728 None:intergenic
GTGTTTGCAGGTGATTCAGT+GGG 0.590354 1:+60489766 MsG0180003315.01.T01:CDS
AAGGTTATCGTAAATGGAGA+TGG 0.591107 1:+60488445 MsG0180003315.01.T01:CDS
TGTGTTTGCAGGTGATTCAG+TGG 0.591622 1:+60489765 MsG0180003315.01.T01:CDS
TTAGGAGATCCTTACAGACA+TGG 0.594474 1:+60490391 MsG0180003315.01.T01:CDS
TGCAAGTAACATACCTTGGA+AGG 0.594886 1:-60488485 None:intergenic
CTATTTCCAGGAAGGTGGGA+AGG 0.609686 1:+60490338 MsG0180003315.01.T01:CDS
TCAGACTTGATGAGTTACAT+TGG 0.612151 1:+60490007 MsG0180003315.01.T01:CDS
TGGTATTCTAATAACTGGAA+GGG 0.614654 1:+60490027 MsG0180003315.01.T01:CDS
AGGGGTATCTATTTCCAGGA+AGG 0.619562 1:+60490330 MsG0180003315.01.T01:CDS
CTTACAGACATGGAAATCAT+GGG 0.626328 1:+60490401 MsG0180003315.01.T01:CDS
ATGCCAGTGACCTCCTAGAG+AGG 0.628848 1:+60489725 MsG0180003315.01.T01:CDS
AAGTCCCATAAGCAAACACA+AGG 0.634961 1:+60489867 MsG0180003315.01.T01:CDS
ATACTTGCCTGTAATGAACA+GGG 0.637425 1:-60490476 MsG0180003316.01.T01:intron
CAAAATGGAAGGAACGACGA+AGG 0.641951 1:+60488426 MsG0180003315.01.T01:CDS
AGAAATAACACATCTAAACA+AGG 0.666678 1:+60490294 MsG0180003315.01.T01:intron
CATTGCGACTCCTCTCTAGG+AGG 0.680185 1:-60489735 None:intergenic
TACTTGCCTGTAATGAACAG+GGG 0.685669 1:-60490475 MsG0180003316.01.T01:intron
AGTCCCATAAGCAAACACAA+GGG 0.771567 1:+60489868 MsG0180003315.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TCAAAAATATATGAAGTTAA+TGG + Chr1:60489844-60489863 MsG0180003315.01.T01:CDS 15.0%
!! TTTATTATGGAAAAATGTTA+AGG + Chr1:60488885-60488904 MsG0180003315.01.T01:intron 15.0%
!!! ATAATAGTCTTTGTTTATTA+TGG + Chr1:60488872-60488891 MsG0180003315.01.T01:intron 15.0%
!!! TATTAAGATTTGATTTGAAA+TGG - Chr1:60489019-60489038 None:intergenic 15.0%
!! AAAACTAGTAACAAAACAAA+TGG - Chr1:60488608-60488627 None:intergenic 20.0%
!! TAAGGACTTAAATTGTTTAT+TGG + Chr1:60488903-60488922 MsG0180003315.01.T01:intron 20.0%
!! TAATTGGAAATAGTCAATAA+TGG + Chr1:60488716-60488735 MsG0180003315.01.T01:intron 20.0%
!! TTATAGAAACAAAATACTAG+TGG + Chr1:60488768-60488787 MsG0180003315.01.T01:intron 20.0%
!!! AATGTTCTTGTTTTCAATAA+TGG + Chr1:60490054-60490073 MsG0180003315.01.T01:CDS 20.0%
!!! GATGTGTTATTTCTATAAAA+AGG - Chr1:60490288-60490307 None:intergenic 20.0%
!!! GTATTTTGTTTCTATAATCA+AGG - Chr1:60488765-60488784 None:intergenic 20.0%
!!! GTTTGTTTGAAATTATTTAG+AGG + Chr1:60489339-60489358 MsG0180003315.01.T01:intron 20.0%
!!! TAACTTCATATATTTTTGAG+AGG - Chr1:60489843-60489862 None:intergenic 20.0%
!!! TTTGTTTTCTAAGAAATGAA+AGG - Chr1:60490171-60490190 None:intergenic 20.0%
! AATTTATCTCAAGTTACTCT+CGG - Chr1:60489146-60489165 None:intergenic 25.0%
! ACATGACTGTAGAATATTAT+AGG + Chr1:60489920-60489939 MsG0180003315.01.T01:CDS 25.0%
! AGAAATAACACATCTAAACA+AGG + Chr1:60490294-60490313 MsG0180003315.01.T01:intron 25.0%
! ATACATGCAATGAAATAGAA+AGG - Chr1:60488546-60488565 None:intergenic 25.0%
! ATACTTAGTGATGAAATTTG+AGG + Chr1:60489891-60489910 MsG0180003315.01.T01:CDS 25.0%
! CATGACTGTAGAATATTATA+GGG + Chr1:60489921-60489940 MsG0180003315.01.T01:CDS 25.0%
! CGAAGGTTTAATATGTAAAA+TGG + Chr1:60488830-60488849 MsG0180003315.01.T01:intron 25.0%
! GGGTTAGAAACAAAATATAT+TGG + Chr1:60488958-60488977 MsG0180003315.01.T01:intron 25.0%
! TACATGCAATGAAATAGAAA+GGG - Chr1:60488545-60488564 None:intergenic 25.0%
! TACATTGGTATTCTAATAAC+TGG + Chr1:60490022-60490041 MsG0180003315.01.T01:CDS 25.0%
! TGACCTATAATAGATAAGAA+AGG - Chr1:60489949-60489968 None:intergenic 25.0%
!! ACTCAATTCAAACTTTTGAT+TGG + Chr1:60489535-60489554 MsG0180003315.01.T01:intron 25.0%
!! AGTGATTTTCGTGAATTATT+GGG - Chr1:60488291-60488310 None:intergenic 25.0%
!! TAGTGATTTTCGTGAATTAT+TGG - Chr1:60488292-60488311 None:intergenic 25.0%
!! TCAAAACGAAAAAGAGTTTT+TGG - Chr1:60488249-60488268 None:intergenic 25.0%
!! TTGTTTTCAATAATGGTCAT+TGG + Chr1:60490061-60490080 MsG0180003315.01.T01:CDS 25.0%
!!! TACTTTGAGATCATTTCATT+TGG + Chr1:60490135-60490154 MsG0180003315.01.T01:intron 25.0%
ACACACTCTCTATTACTATT+TGG - Chr1:60489439-60489458 None:intergenic 30.0%
AGAGTATGACACTAGTTAAA+AGG - Chr1:60489637-60489656 None:intergenic 30.0%
ATCCGTTCAAGAAAATTTGA+CGG + Chr1:60489109-60489128 MsG0180003315.01.T01:intron 30.0%
GAAAAATCACATGCTTCAAT+TGG - Chr1:60488315-60488334 None:intergenic 30.0%
GCACCTTTCTTATCTATTAT+AGG + Chr1:60489943-60489962 MsG0180003315.01.T01:CDS 30.0%
TACTTTGCATGTTGTCAAAA+GGG + Chr1:60489809-60489828 MsG0180003315.01.T01:CDS 30.0%
TGAGTAATCTAAATCCTATG+AGG + Chr1:60490427-60490446 MsG0180003315.01.T01:CDS 30.0%
TGGTATTCTAATAACTGGAA+GGG + Chr1:60490027-60490046 MsG0180003315.01.T01:CDS 30.0%
TTACTTTGCATGTTGTCAAA+AGG + Chr1:60489808-60489827 MsG0180003315.01.T01:CDS 30.0%
TTGGTATTCTAATAACTGGA+AGG + Chr1:60490026-60490045 MsG0180003315.01.T01:CDS 30.0%
! ATCCGTCAAATTTTCTTGAA+CGG - Chr1:60489114-60489133 None:intergenic 30.0%
! TGAGCTTCTAAACAATTTTG+AGG - Chr1:60490209-60490228 None:intergenic 30.0%
! TGATTTTTCCAAAGGTCAAT+GGG + Chr1:60488326-60488345 MsG0180003315.01.T01:CDS 30.0%
! TTTCTAAGAAATGAAAGGCA+AGG - Chr1:60490166-60490185 None:intergenic 30.0%
! TTTTCAATAATGGTCATTGG+TGG + Chr1:60490064-60490083 MsG0180003315.01.T01:CDS 30.0%
! TTTTTCTGTCCTCTCTTTTT+TGG + Chr1:60488683-60488702 MsG0180003315.01.T01:intron 30.0%
!! AGAGTTTTTGGATCAAAGTT+AGG - Chr1:60488237-60488256 None:intergenic 30.0%
!! CAATTCAAACTTTTGATTGG+TGG + Chr1:60489538-60489557 MsG0180003315.01.T01:intron 30.0%
!! GAGTTTTTGGATCAAAGTTA+GGG - Chr1:60488236-60488255 None:intergenic 30.0%
!! TTTTGGAGAAGTAGGTTAAT+TGG + Chr1:60488700-60488719 MsG0180003315.01.T01:intron 30.0%
!!! TCTTATTTTCCTTTCCAGAT+GGG + Chr1:60488800-60488819 MsG0180003315.01.T01:intron 30.0%
AAATGTTACACTATGTGTCG+TGG - Chr1:60489284-60489303 None:intergenic 35.0%
AACATGCAAGTAACATACCT+TGG - Chr1:60488492-60488511 None:intergenic 35.0%
AAGGTTATCGTAAATGGAGA+TGG + Chr1:60488445-60488464 MsG0180003315.01.T01:CDS 35.0%
ACTTTGCATGTTGTCAAAAG+GGG + Chr1:60489810-60489829 MsG0180003315.01.T01:CDS 35.0%
ATACTTGCCTGTAATGAACA+GGG - Chr1:60490479-60490498 MsG0180003316.01.T01:intron 35.0%
CAATCAAGGTAAAAGTGAGA+TGG - Chr1:60489058-60489077 None:intergenic 35.0%
CATGGAAATAGGAGAATAAG+AGG + Chr1:60488739-60488758 MsG0180003315.01.T01:intron 35.0%
CTTACAGACATGGAAATCAT+GGG + Chr1:60490401-60490420 MsG0180003315.01.T01:CDS 35.0%
GGAAATAGTCAATAATGGCA+TGG + Chr1:60488721-60488740 MsG0180003315.01.T01:intron 35.0%
GTCAATAATGGCATGGAAAT+AGG + Chr1:60488728-60488747 MsG0180003315.01.T01:intron 35.0%
TATACTTGCCTGTAATGAAC+AGG - Chr1:60490480-60490499 MsG0180003316.01.T01:intron 35.0%
TATCCCTTGTGTTTGCTTAT+GGG - Chr1:60489874-60489893 None:intergenic 35.0%
TCAGACTTGATGAGTTACAT+TGG + Chr1:60490007-60490026 MsG0180003315.01.T01:CDS 35.0%
TCTAACCCATGTATGTAGTT+GGG - Chr1:60488946-60488965 None:intergenic 35.0%
TGTGTCGTGGCTATAAATTT+CGG - Chr1:60489271-60489290 None:intergenic 35.0%
TTCTAACCCATGTATGTAGT+TGG - Chr1:60488947-60488966 None:intergenic 35.0%
! AACAAGGTCTATCATTTTGC+AGG + Chr1:60490310-60490329 MsG0180003315.01.T01:CDS 35.0%
! ACAAGGTCTATCATTTTGCA+GGG + Chr1:60490311-60490330 MsG0180003315.01.T01:CDS 35.0%
! ATGTCACTAGATGAGTTTTG+TGG - Chr1:60489976-60489995 None:intergenic 35.0%
! GAAGCATGTGATTTTTCCAA+AGG + Chr1:60488318-60488337 MsG0180003315.01.T01:CDS 35.0%
! GTGATTTTTCCAAAGGTCAA+TGG + Chr1:60488325-60488344 MsG0180003315.01.T01:CDS 35.0%
! TGACAGTGAAAGAAGCTTTT+AGG + Chr1:60490373-60490392 MsG0180003315.01.T01:CDS 35.0%
! TTTTTGGATCAAAGTTAGGG+AGG - Chr1:60488233-60488252 None:intergenic 35.0%
!! CAAAATACTAGTGGACTACT+AGG + Chr1:60488777-60488796 MsG0180003315.01.T01:intron 35.0%
!! CTTATTTTCCTTTCCAGATG+GGG + Chr1:60488801-60488820 MsG0180003315.01.T01:intron 35.0%
!!! GTCTTATTTTCCTTTCCAGA+TGG + Chr1:60488799-60488818 MsG0180003315.01.T01:intron 35.0%
AAGAAGACGAAACTCCTCAT+AGG - Chr1:60490444-60490463 MsG0180003316.01.T01:three_prime_UTR 40.0%
AAGTCCCATAAGCAAACACA+AGG + Chr1:60489867-60489886 MsG0180003315.01.T01:CDS 40.0%
AATTGTCCCTTTCTTGATCC+CGG + Chr1:60488390-60488409 MsG0180003315.01.T01:CDS 40.0%
ACCTCTTATCGCAACAAACA+TGG + Chr1:60488651-60488670 MsG0180003315.01.T01:intron 40.0%
ACGACGAAGGTTATCGTAAA+TGG + Chr1:60488439-60488458 MsG0180003315.01.T01:CDS 40.0%
AGTCCCATAAGCAAACACAA+GGG + Chr1:60489868-60489887 MsG0180003315.01.T01:CDS 40.0%
ATGTAGTTGGGCACATACAT+GGG - Chr1:60488934-60488953 None:intergenic 40.0%
ATGTGCCCAACTACATACAT+GGG + Chr1:60488938-60488957 MsG0180003315.01.T01:intron 40.0%
CCATGATTTCCATGTCTGTA+AGG - Chr1:60490403-60490422 MsG0180003316.01.T01:three_prime_UTR 40.0%
CCTACTTCTCCAAAAAAGAG+AGG - Chr1:60488695-60488714 None:intergenic 40.0%
CCTTACAGACATGGAAATCA+TGG + Chr1:60490400-60490419 MsG0180003315.01.T01:CDS 40.0%
CTAATGATTGTGACCTTCCA+AGG + Chr1:60488472-60488491 MsG0180003315.01.T01:CDS 40.0%
CTTGGAAGGTCACAATCATT+AGG - Chr1:60488474-60488493 None:intergenic 40.0%
GCATGCAACTATTGCAATCA+AGG - Chr1:60489072-60489091 None:intergenic 40.0%
GTATCCCTTGTGTTTGCTTA+TGG - Chr1:60489875-60489894 None:intergenic 40.0%
GTATCTATTTCCAGGAAGGT+GGG + Chr1:60490334-60490353 MsG0180003315.01.T01:CDS 40.0%
TACTTGCCTGTAATGAACAG+GGG - Chr1:60490478-60490497 MsG0180003316.01.T01:intron 40.0%
TATGTAGTTGGGCACATACA+TGG - Chr1:60488935-60488954 None:intergenic 40.0%
TATGTGCCCAACTACATACA+TGG + Chr1:60488937-60488956 MsG0180003315.01.T01:intron 40.0%
TCATTGCATGTATGCATGCA+TGG + Chr1:60488553-60488572 MsG0180003315.01.T01:intron 40.0%
TGCAAAGTAATGACTCCCAT+TGG - Chr1:60489798-60489817 None:intergenic 40.0%
TGCAAGTAACATACCTTGGA+AGG - Chr1:60488488-60488507 None:intergenic 40.0%
TTAGGAGATCCTTACAGACA+TGG + Chr1:60490391-60490410 MsG0180003315.01.T01:CDS 40.0%
! CAAGGTCTATCATTTTGCAG+GGG + Chr1:60490312-60490331 MsG0180003315.01.T01:CDS 40.0%
! TTCCAAAGGTCAATGGGTTT+GGG + Chr1:60488332-60488351 MsG0180003315.01.T01:CDS 40.0%
! TTTCCAAAGGTCAATGGGTT+TGG + Chr1:60488331-60488350 MsG0180003315.01.T01:CDS 40.0%
!! TCACTAGATGAGTTTTGTGG+TGG - Chr1:60489973-60489992 None:intergenic 40.0%
!!! CCTCTCTTTTTTGGAGAAGT+AGG + Chr1:60488692-60488711 MsG0180003315.01.T01:intron 40.0%
AATGGACGCATTGTGTTTGC+AGG + Chr1:60489754-60489773 MsG0180003315.01.T01:CDS 45.0%
ATTCAGTGGGCAGAAACCAA+TGG + Chr1:60489779-60489798 MsG0180003315.01.T01:CDS 45.0%
CAAAATGGAAGGAACGACGA+AGG + Chr1:60488426-60488445 MsG0180003315.01.T01:CDS 45.0%
GGTATCTATTTCCAGGAAGG+TGG + Chr1:60490333-60490352 MsG0180003315.01.T01:CDS 45.0%
GTGTTTGCAGGTGATTCAGT+GGG + Chr1:60489766-60489785 MsG0180003315.01.T01:CDS 45.0%
TGTGTTTGCAGGTGATTCAG+TGG + Chr1:60489765-60489784 MsG0180003315.01.T01:CDS 45.0%
TTCAGTGGGCAGAAACCAAT+GGG + Chr1:60489780-60489799 MsG0180003315.01.T01:CDS 45.0%
! GCCATGTTTGTTGCGATAAG+AGG - Chr1:60488655-60488674 None:intergenic 45.0%
!! AGGGGTATCTATTTCCAGGA+AGG + Chr1:60490330-60490349 MsG0180003315.01.T01:CDS 45.0%
!! TTGCAGGGGTATCTATTTCC+AGG + Chr1:60490326-60490345 MsG0180003315.01.T01:CDS 45.0%
ACCTTCGAAAACACCCCATC+TGG - Chr1:60488817-60488836 None:intergenic 50.0%
CATTTACCTTCCCACCTTCC+TGG - Chr1:60490347-60490366 MsG0180003316.01.T01:three_prime_UTR 50.0%
CCTTTCTTGATCCCGGATTC+CGG + Chr1:60488397-60488416 MsG0180003315.01.T01:CDS 50.0%
CGAAAACACCCCATCTGGAA+AGG - Chr1:60488812-60488831 None:intergenic 50.0%
CGGAATCCGGGATCAAGAAA+GGG - Chr1:60488399-60488418 None:intergenic 50.0%
CTATTTCCAGGAAGGTGGGA+AGG + Chr1:60490338-60490357 MsG0180003315.01.T01:CDS 50.0%
GCTATGCCCCTGTTCATTAC+AGG + Chr1:60490469-60490488 MsG0180003315.01.T01:CDS 50.0%
GTCCCAAACCCATTGACCTT+TGG - Chr1:60488337-60488356 None:intergenic 50.0%
! CGTCGTTCCTTCCATTTTGC+CGG - Chr1:60488425-60488444 None:intergenic 50.0%
!! TCCAGATGGGGTGTTTTCGA+AGG + Chr1:60488813-60488832 MsG0180003315.01.T01:intron 50.0%
ATGCCAGTGACCTCCTAGAG+AGG + Chr1:60489725-60489744 MsG0180003315.01.T01:CDS 55.0%
CATTGCGACTCCTCTCTAGG+AGG - Chr1:60489738-60489757 None:intergenic 55.0%
CCGGAATCCGGGATCAAGAA+AGG - Chr1:60488400-60488419 None:intergenic 55.0%
CTCCTAGAGAGGAGTCGCAA+TGG + Chr1:60489736-60489755 MsG0180003315.01.T01:CDS 55.0%
GTCCATTGCGACTCCTCTCT+AGG - Chr1:60489741-60489760 None:intergenic 55.0%
! ACTCCTCTCTAGGAGGTCAC+TGG - Chr1:60489731-60489750 None:intergenic 55.0%
! TCCTTCCATTTTGCCGGCAC+CGG - Chr1:60488419-60488438 None:intergenic 55.0%
!! ATTTTGCCGGCACCGGAATC+CGG - Chr1:60488412-60488431 None:intergenic 55.0%
TTGATCCCGGATTCCGGTGC+CGG + Chr1:60488403-60488422 MsG0180003315.01.T01:CDS 60.0%
!! GGATTCCGGTGCCGGCAAAA+TGG + Chr1:60488411-60488430 MsG0180003315.01.T01:CDS 60.0%
!! TCCGGTGCCGGCAAAATGGA+AGG + Chr1:60488415-60488434 MsG0180003315.01.T01:CDS 60.0%
!! TTTTGCCGGCACCGGAATCC+GGG - Chr1:60488411-60488430 None:intergenic 60.0%
Chromosome Type Strat End Strand Name
Chr1 gene 60488162 60490503 60488162 ID=MsG0180003315.01;Name=MsG0180003315.01
Chr1 mRNA 60488162 60490503 60488162 ID=MsG0180003315.01.T01;Parent=MsG0180003315.01;Name=MsG0180003315.01.T01;_AED=0.26;_eAED=0.26;_QI=0|0|0|1|1|1|3|0|309
Chr1 exon 60488162 60488493 60488162 ID=MsG0180003315.01.T01:exon:49338;Parent=MsG0180003315.01.T01
Chr1 exon 60489720 60490109 60489720 ID=MsG0180003315.01.T01:exon:49339;Parent=MsG0180003315.01.T01
Chr1 exon 60490296 60490503 60490296 ID=MsG0180003315.01.T01:exon:49340;Parent=MsG0180003315.01.T01
Chr1 CDS 60488162 60488493 60488162 ID=MsG0180003315.01.T01:cds;Parent=MsG0180003315.01.T01
Chr1 CDS 60489720 60490109 60489720 ID=MsG0180003315.01.T01:cds;Parent=MsG0180003315.01.T01
Chr1 CDS 60490296 60490503 60490296 ID=MsG0180003315.01.T01:cds;Parent=MsG0180003315.01.T01
Gene Sequence

>MsG0180003315.01.T01

ATGAATAGAGAATTAGCATATGCACTCTCTTTTCTTTTTGTCTTACTATCTTCTCTATTCTATTTCAACCTCCCTAACTTTGATCCAAAAACTCTTTTTCGTTTTGATTTCTTGTCACGTACAGCTCCCAATAATTCACGAAAATCACTACCAATTGAAGCATGTGATTTTTCCAAAGGTCAATGGGTTTGGGACGAAACTTATTATTCACGTAAGTTGTATGATGAAAATTGTCCCTTTCTTGATCCCGGATTCCGGTGCCGGCAAAATGGAAGGAACGACGAAGGTTATCGTAAATGGAGATGGCAACCTAATGATTGTGACCTTCCAAGATTCAATGCCAGTGACCTCCTAGAGAGGAGTCGCAATGGACGCATTGTGTTTGCAGGTGATTCAGTGGGCAGAAACCAATGGGAGTCATTACTTTGCATGTTGTCAAAAGGGGTCTCTAACCTCTCAAAAATATATGAAGTTAATGGAAGTCCCATAAGCAAACACAAGGGATACTTAGTGATGAAATTTGAGGATTACAACATGACTGTAGAATATTATAGGGCACCTTTCTTATCTATTATAGGTCATCCACCACAAAACTCATCTAGTGACATTAAAATGACTATCAGACTTGATGAGTTACATTGGTATTCTAATAACTGGAAGGGAGCAAATGTTCTTGTTTTCAATAATGGTCATTGGTGGAACTTAGACAAAACTATCAAGTCAAATAACACATCTAAACAAGGTCTATCATTTTGCAGGGGTATCTATTTCCAGGAAGGTGGGAAGGTAAATGTGACTATGACAGTGAAAGAAGCTTTTAGGAGATCCTTACAGACATGGAAATCATGGGCATTGAGTAATCTAAATCCTATGAGGAGTTTCGTCTTCTTTCGTAGCTATGCCCCTGTTCATTACAGGCAAGTATATTAA
Protein sequence

>MsG0180003315.01.T01

MNRELAYALSFLFVLLSSLFYFNLPNFDPKTLFRFDFLSRTAPNNSRKSLPIEACDFSKGQWVWDETYYSRKLYDENCPFLDPGFRCRQNGRNDEGYRKWRWQPNDCDLPRFNASDLLERSRNGRIVFAGDSVGRNQWESLLCMLSKGVSNLSKIYEVNGSPISKHKGYLVMKFEDYNMTVEYYRAPFLSIIGHPPQNSSSDIKMTIRLDELHWYSNNWKGANVLVFNNGHWWNLDKTIKSNNTSKQGLSFCRGIYFQEGGKVNVTMTVKEAFRRSLQTWKSWALSNLNPMRSFVFFRSYAPVHYRQVY*