Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180003348.01.T01 | XP_013467980.1 | 93.548 | 155 | 10 | 0 | 1 | 155 | 1 | 155 | 3.22E-100 | 299 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180003348.01.T01 | I3T181 | 93.548 | 155 | 10 | 0 | 1 | 155 | 1 | 155 | 1.54e-100 | 299 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180003338.01 | MsG0180003348.01 | 0.897766 | 1.022482e-76 | 1.172728e-72 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180003348.01.T01 | MTR_1g059890 | 93.548 | 155 | 10 | 0 | 1 | 155 | 1 | 155 | 3.90e-104 | 299 |
| MsG0180003348.01.T01 | MTR_7g104725 | 49.133 | 173 | 57 | 8 | 4 | 155 | 2 | 164 | 5.74e-33 | 118 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180003348.01.T01 | AT2G36220 | 43.636 | 165 | 67 | 8 | 1 | 148 | 1 | 156 | 1.34e-23 | 93.6 |
| MsG0180003348.01.T01 | AT3G52710 | 42.690 | 171 | 63 | 11 | 1 | 149 | 1 | 158 | 2.33e-22 | 90.5 |
Find 49 sgRNAs with CRISPR-Local
Find 49 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGAAGCGGAGTTAGGGTTTA+TGG | 0.148476 | 1:-60970945 | None:intergenic |
| TTGGTTCGCAAGCGACTTTC+CGG | 0.240044 | 1:-60970802 | None:intergenic |
| ACTCACCACCGGAATCATTT+TGG | 0.243921 | 1:+60970840 | MsG0180003348.01.T01:CDS |
| TTTGGATTTCTTCAAATTCT+TGG | 0.266541 | 1:-60970984 | None:intergenic |
| TCACCGAAGGTTTCTTGTAT+AGG | 0.328962 | 1:+60971166 | MsG0180003348.01.T01:CDS |
| TGAGAGTTTGAAGCGGAGTT+AGG | 0.333425 | 1:-60970953 | None:intergenic |
| TCTGTCCCACCAGTCGTATT+CGG | 0.335672 | 1:-60970876 | None:intergenic |
| ACCGATGATTGTCGTAGCTT+TGG | 0.337880 | 1:-60971002 | None:intergenic |
| AACGTAGCTTTCTGTGATTT+CGG | 0.349811 | 1:-60971028 | None:intergenic |
| CCGATCGACGCGCTTCGTTT+CGG | 0.362918 | 1:-60971109 | None:intergenic |
| CTACGATATTATGCCACAAC+TGG | 0.399202 | 1:+60970736 | None:intergenic |
| GTCGGCGGCGACGCCGGATA+AGG | 0.399937 | 1:-60970780 | None:intergenic |
| CGCCGGATAAGGCGGAAACT+AGG | 0.401389 | 1:-60970769 | None:intergenic |
| ACCCTAGTTTCCGCCTTATC+CGG | 0.413514 | 1:+60970767 | MsG0180003348.01.T01:CDS |
| TTGGAGAGCCAAAATGATTC+CGG | 0.437316 | 1:-60970848 | None:intergenic |
| TAGGTAGAGAAAGTAAACTT+AGG | 0.454703 | 1:+60971185 | MsG0180003348.01.T01:CDS |
| AGGTAGAGAAAGTAAACTTA+GGG | 0.472710 | 1:+60971186 | MsG0180003348.01.T01:CDS |
| CAAGAAACCTTCGGTGAAGA+AGG | 0.478039 | 1:-60971160 | None:intergenic |
| GAGAGTTTGAAGCGGAGTTA+GGG | 0.479960 | 1:-60970952 | None:intergenic |
| TATCCGGCGTCGCCGCCGAC+CGG | 0.484565 | 1:+60970783 | MsG0180003348.01.T01:CDS |
| TTTCCGGTCGGCGGCGACGC+CGG | 0.485968 | 1:-60970786 | None:intergenic |
| GAGTCTTCCGCTTCATCCGT+TGG | 0.498389 | 1:-60970821 | None:intergenic |
| CCGTCGGAATCATCACAAGC+TGG | 0.517625 | 1:+60971069 | MsG0180003348.01.T01:CDS |
| GCCGGATAAGGCGGAAACTA+GGG | 0.517835 | 1:-60970768 | None:intergenic |
| TGATTCGTTTCGTTCGTAGA+CGG | 0.519502 | 1:-60970903 | None:intergenic |
| TCCAAAGCTACGACAATCAT+CGG | 0.538829 | 1:+60971001 | MsG0180003348.01.T01:CDS |
| CCAACGATGCCGAATACGAC+TGG | 0.551928 | 1:+60970867 | MsG0180003348.01.T01:CDS |
| CTACCTATACAAGAAACCTT+CGG | 0.562410 | 1:-60971169 | None:intergenic |
| TTCGCAAGCGACTTTCCGGT+CGG | 0.571303 | 1:-60970798 | None:intergenic |
| CCGAAACGAAGCGCGTCGAT+CGG | 0.572731 | 1:+60971109 | MsG0180003348.01.T01:CDS |
| CGTCGGAATCATCACAAGCT+GGG | 0.575578 | 1:+60971070 | MsG0180003348.01.T01:CDS |
| CCAGTCGTATTCGGCATCGT+TGG | 0.587629 | 1:-60970867 | None:intergenic |
| ACGATGCCGAATACGACTGG+TGG | 0.592278 | 1:+60970870 | MsG0180003348.01.T01:CDS |
| GGCGGCGACGCCGGATAAGG+CGG | 0.594812 | 1:-60970777 | None:intergenic |
| GCAAGCGACTTTCCGGTCGG+CGG | 0.604992 | 1:-60970795 | None:intergenic |
| CCAGCTTGTGATGATTCCGA+CGG | 0.609518 | 1:-60971069 | None:intergenic |
| AAACGAAGCGCGTCGATCGG+AGG | 0.611700 | 1:+60971112 | MsG0180003348.01.T01:CDS |
| TTGCGAACCAACGGATGAAG+CGG | 0.617781 | 1:+60970814 | MsG0180003348.01.T01:CDS |
| GCTACGTTAGCAGAAGCTAG+AGG | 0.623845 | 1:+60971043 | MsG0180003348.01.T01:CDS |
| GAAAGTCGCTTGCGAACCAA+CGG | 0.631019 | 1:+60970805 | MsG0180003348.01.T01:CDS |
| CGATGCCGAATACGACTGGT+GGG | 0.649006 | 1:+60970871 | MsG0180003348.01.T01:CDS |
| GAATCGTTGAGAGTTTGAAG+CGG | 0.651586 | 1:-60970960 | None:intergenic |
| GGTAGAGAAAGTAAACTTAG+GGG | 0.652293 | 1:+60971187 | MsG0180003348.01.T01:CDS |
| GTAGAGAAAGTAAACTTAGG+GGG | 0.653094 | 1:+60971188 | MsG0180003348.01.T01:CDS |
| CAGAAGCTAGAGGCTGCCGT+CGG | 0.668462 | 1:+60971053 | MsG0180003348.01.T01:CDS |
| GAGAGCCAAAATGATTCCGG+TGG | 0.680383 | 1:-60970845 | None:intergenic |
| TGAAGCGGAAGACTCACCAC+CGG | 0.695803 | 1:+60970829 | MsG0180003348.01.T01:CDS |
| AGGGTTTCGAAATCCAGTTG+TGG | 0.701704 | 1:-60970749 | None:intergenic |
| ATCACAAGCTGGGAAACACG+AGG | 0.718195 | 1:+60971080 | MsG0180003348.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | TTTGGATTTCTTCAAATTCT+TGG | - | Chr1:60970987-60971006 | None:intergenic | 25.0% |
| AGGTAGAGAAAGTAAACTTA+GGG | + | Chr1:60971186-60971205 | MsG0180003348.01.T01:CDS | 30.0% | |
| TAGGTAGAGAAAGTAAACTT+AGG | + | Chr1:60971185-60971204 | MsG0180003348.01.T01:CDS | 30.0% | |
| AACGTAGCTTTCTGTGATTT+CGG | - | Chr1:60971031-60971050 | None:intergenic | 35.0% | |
| CTACCTATACAAGAAACCTT+CGG | - | Chr1:60971172-60971191 | None:intergenic | 35.0% | |
| GGTAGAGAAAGTAAACTTAG+GGG | + | Chr1:60971187-60971206 | MsG0180003348.01.T01:CDS | 35.0% | |
| GTAGAGAAAGTAAACTTAGG+GGG | + | Chr1:60971188-60971207 | MsG0180003348.01.T01:CDS | 35.0% | |
| GAATCGTTGAGAGTTTGAAG+CGG | - | Chr1:60970963-60970982 | None:intergenic | 40.0% | |
| TCACCGAAGGTTTCTTGTAT+AGG | + | Chr1:60971166-60971185 | MsG0180003348.01.T01:CDS | 40.0% | |
| TCCAAAGCTACGACAATCAT+CGG | + | Chr1:60971001-60971020 | MsG0180003348.01.T01:CDS | 40.0% | |
| TGATTCGTTTCGTTCGTAGA+CGG | - | Chr1:60970906-60970925 | None:intergenic | 40.0% | |
| TTGGAGAGCCAAAATGATTC+CGG | - | Chr1:60970851-60970870 | None:intergenic | 40.0% | |
| ! | TTTTGAACCTTCTTCACCGA+AGG | + | Chr1:60971153-60971172 | MsG0180003348.01.T01:CDS | 40.0% |
| ACCGATGATTGTCGTAGCTT+TGG | - | Chr1:60971005-60971024 | None:intergenic | 45.0% | |
| AGGGTTTCGAAATCCAGTTG+TGG | - | Chr1:60970752-60970771 | None:intergenic | 45.0% | |
| CAAGAAACCTTCGGTGAAGA+AGG | - | Chr1:60971163-60971182 | None:intergenic | 45.0% | |
| TGAAGCGGAGTTAGGGTTTA+TGG | - | Chr1:60970948-60970967 | None:intergenic | 45.0% | |
| ! | ACTCACCACCGGAATCATTT+TGG | + | Chr1:60970840-60970859 | MsG0180003348.01.T01:CDS | 45.0% |
| ! | GAGAGTTTGAAGCGGAGTTA+GGG | - | Chr1:60970955-60970974 | None:intergenic | 45.0% |
| ! | TGAGAGTTTGAAGCGGAGTT+AGG | - | Chr1:60970956-60970975 | None:intergenic | 45.0% |
| ACCCTAGTTTCCGCCTTATC+CGG | + | Chr1:60970767-60970786 | MsG0180003348.01.T01:CDS | 50.0% | |
| ATCACAAGCTGGGAAACACG+AGG | + | Chr1:60971080-60971099 | MsG0180003348.01.T01:CDS | 50.0% | |
| CCAGCTTGTGATGATTCCGA+CGG | - | Chr1:60971072-60971091 | None:intergenic | 50.0% | |
| CGTCGGAATCATCACAAGCT+GGG | + | Chr1:60971070-60971089 | MsG0180003348.01.T01:CDS | 50.0% | |
| GAAAGTCGCTTGCGAACCAA+CGG | + | Chr1:60970805-60970824 | MsG0180003348.01.T01:CDS | 50.0% | |
| GAGAGCCAAAATGATTCCGG+TGG | - | Chr1:60970848-60970867 | None:intergenic | 50.0% | |
| GCTACGTTAGCAGAAGCTAG+AGG | + | Chr1:60971043-60971062 | MsG0180003348.01.T01:CDS | 50.0% | |
| TCTGTCCCACCAGTCGTATT+CGG | - | Chr1:60970879-60970898 | None:intergenic | 50.0% | |
| TTGCGAACCAACGGATGAAG+CGG | + | Chr1:60970814-60970833 | MsG0180003348.01.T01:CDS | 50.0% | |
| ! | TTGGTTCGCAAGCGACTTTC+CGG | - | Chr1:60970805-60970824 | None:intergenic | 50.0% |
| ACGATGCCGAATACGACTGG+TGG | + | Chr1:60970870-60970889 | MsG0180003348.01.T01:CDS | 55.0% | |
| CCAACGATGCCGAATACGAC+TGG | + | Chr1:60970867-60970886 | MsG0180003348.01.T01:CDS | 55.0% | |
| CCAGTCGTATTCGGCATCGT+TGG | - | Chr1:60970870-60970889 | None:intergenic | 55.0% | |
| CCGTCGGAATCATCACAAGC+TGG | + | Chr1:60971069-60971088 | MsG0180003348.01.T01:CDS | 55.0% | |
| CGATGCCGAATACGACTGGT+GGG | + | Chr1:60970871-60970890 | MsG0180003348.01.T01:CDS | 55.0% | |
| GAGTCTTCCGCTTCATCCGT+TGG | - | Chr1:60970824-60970843 | None:intergenic | 55.0% | |
| GCCGGATAAGGCGGAAACTA+GGG | - | Chr1:60970771-60970790 | None:intergenic | 55.0% | |
| TGAAGCGGAAGACTCACCAC+CGG | + | Chr1:60970829-60970848 | MsG0180003348.01.T01:CDS | 55.0% | |
| TTCGCAAGCGACTTTCCGGT+CGG | - | Chr1:60970801-60970820 | None:intergenic | 55.0% | |
| CAGAAGCTAGAGGCTGCCGT+CGG | + | Chr1:60971053-60971072 | MsG0180003348.01.T01:CDS | 60.0% | |
| CCGATCGACGCGCTTCGTTT+CGG | - | Chr1:60971112-60971131 | None:intergenic | 60.0% | |
| CGCCGGATAAGGCGGAAACT+AGG | - | Chr1:60970772-60970791 | None:intergenic | 60.0% | |
| ! | AAACGAAGCGCGTCGATCGG+AGG | + | Chr1:60971112-60971131 | MsG0180003348.01.T01:CDS | 60.0% |
| ! | CCGAAACGAAGCGCGTCGAT+CGG | + | Chr1:60971109-60971128 | MsG0180003348.01.T01:CDS | 60.0% |
| GCAAGCGACTTTCCGGTCGG+CGG | - | Chr1:60970798-60970817 | None:intergenic | 65.0% | |
| GGCGGCGACGCCGGATAAGG+CGG | - | Chr1:60970780-60970799 | None:intergenic | 75.0% | |
| GTCGGCGGCGACGCCGGATA+AGG | - | Chr1:60970783-60970802 | None:intergenic | 75.0% | |
| TATCCGGCGTCGCCGCCGAC+CGG | + | Chr1:60970783-60970802 | MsG0180003348.01.T01:CDS | 75.0% | |
| TTTCCGGTCGGCGGCGACGC+CGG | - | Chr1:60970789-60970808 | None:intergenic | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr1 | gene | 60970746 | 60971225 | 60970746 | ID=MsG0180003348.01;Name=MsG0180003348.01 |
| Chr1 | mRNA | 60970746 | 60971225 | 60970746 | ID=MsG0180003348.01.T01;Parent=MsG0180003348.01;Name=MsG0180003348.01.T01;_AED=0.02;_eAED=0.02;_QI=0|-1|0|1|-1|1|1|0|159 |
| Chr1 | exon | 60970746 | 60971225 | 60970746 | ID=MsG0180003348.01.T01:exon:23261;Parent=MsG0180003348.01.T01 |
| Chr1 | CDS | 60970746 | 60971225 | 60970746 | ID=MsG0180003348.01.T01:cds;Parent=MsG0180003348.01.T01 |
| Gene Sequence |
| Protein sequence |
