AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0180003395.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180003395.01.T01 MTR_1g060860 73.397 312 66 2 1 296 1 311 1.67e-165 462
MsG0180003395.01.T01 MTR_1g060850 92.778 180 13 0 1 180 1 180 9.95e-126 356
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180003395.01.T01 AT3G09970 58.958 307 109 2 4 294 2 307 2.78e-135 385
MsG0180003395.01.T01 AT3G09960 56.536 306 115 3 4 292 3 307 1.26e-125 361
MsG0180003395.01.T01 AT3G09970 56.522 253 93 2 4 240 2 253 4.30e-105 307

Find 81 sgRNAs with CRISPR-Local

Find 237 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TAACAATTCTGAAAGCATTT+TGG 0.175375 1:+61632527 MsG0180003395.01.T01:intron
CGCAGGGTGGCGGGAGGAGG+TGG 0.275927 1:-61630194 None:intergenic
GAGAGAGAAGGGTGGTTTAC+TGG 0.276231 1:+61630264 MsG0180003395.01.T01:CDS
ACAGCCATTATCATCTTCTT+AGG 0.288773 1:+61630042 MsG0180003395.01.T01:CDS
ATTCGTGATACAGATCTATT+AGG 0.313950 1:+61632980 MsG0180003395.01.T01:CDS
AACTCCATGTTGAAGGCTTA+AGG 0.360440 1:+61632885 MsG0180003395.01.T01:CDS
GGAAGAATAATTGCAGCTAT+TGG 0.364132 1:-61632944 None:intergenic
AGCTTGAACCATAGGACTTC+GGG 0.368046 1:-61632686 None:intergenic
CGTCGCAGGGTGGCGGGAGG+AGG 0.368315 1:-61630197 None:intergenic
TGTATCACGAATAATCTTCA+TGG 0.369384 1:-61632969 None:intergenic
AAGTTGATTGCTGTCCATGC+TGG 0.382258 1:+61632607 MsG0180003395.01.T01:CDS
AGGGTGGCGGGAGGAGGTGG+AGG 0.385820 1:-61630191 None:intergenic
TAAGTGGTAGAAAGAATGTA+TGG 0.406045 1:+61632710 MsG0180003395.01.T01:CDS
CGTTCTTGTATGCTTTGGTT+TGG 0.416734 1:-61629885 None:intergenic
CTCCTCCCGCCACCCTGCGA+CGG 0.424125 1:+61630198 MsG0180003395.01.T01:CDS
CTATAAGAAAATCAATTACT+TGG 0.429125 1:-61630095 None:intergenic
GATTCTGACATTGTTGTTGT+TGG 0.432094 1:-61629910 None:intergenic
AGTTCAACCATCATTGTTAG+TGG 0.435708 1:+61632851 MsG0180003395.01.T01:CDS
ATCACCTAAGAAGATGATAA+TGG 0.445960 1:-61630046 None:intergenic
AGAAAATTCTGACCCGTCGC+AGG 0.447690 1:-61630211 None:intergenic
AGCATACGACCCTTGCTTCA+TGG 0.447806 1:-61629933 None:intergenic
GACTAAATTTAATGTTGCTA+AGG 0.450224 1:+61630341 MsG0180003395.01.T01:CDS
AGCATTGTGAAGAGAGAGAA+GGG 0.452098 1:+61630253 MsG0180003395.01.T01:CDS
GAGGTGGAGGAAGGCGGCGA+AGG 0.455694 1:-61630178 None:intergenic
CGGGAGGAGGTGGAGGAAGG+CGG 0.456011 1:-61630184 None:intergenic
TCTGACCCGTCGCAGGGTGG+CGG 0.457196 1:-61630204 None:intergenic
CGAAGTCATGGTTTCCAGCT+AGG 0.462451 1:-61630155 None:intergenic
TGGTTCGTTCTTGTATGCTT+TGG 0.467489 1:-61629890 None:intergenic
TCCTCCCGCCACCCTGCGAC+GGG 0.467836 1:+61630199 MsG0180003395.01.T01:CDS
AAGAAAATCAATTACTTGGC+GGG 0.469881 1:-61630091 None:intergenic
CATGCAAAACTCCATGTTGA+AGG 0.471442 1:+61632878 MsG0180003395.01.T01:CDS
GCATACGACCCTTGCTTCAT+GGG 0.476270 1:-61629932 None:intergenic
GTCTTTATAAACATTGATGA+TGG 0.479812 1:+61632571 MsG0180003395.01.T01:CDS
CTGACCCGTCGCAGGGTGGC+GGG 0.487506 1:-61630203 None:intergenic
TCGAGATACCCGAAGTCCTA+TGG 0.490954 1:+61632678 MsG0180003395.01.T01:CDS
CAGTCCTACGGAGTTTCTCA+TGG 0.499977 1:+61630408 MsG0180003395.01.T01:CDS
ACTAAATTTAATGTTGCTAA+GGG 0.501234 1:+61630342 MsG0180003395.01.T01:CDS
AAGCATTGTGAAGAGAGAGA+AGG 0.507879 1:+61630252 MsG0180003395.01.T01:CDS
CCACTTAGAGCTTGAACCAT+AGG 0.508217 1:-61632694 None:intergenic
TAAGAAAATCAATTACTTGG+CGG 0.510428 1:-61630092 None:intergenic
TTAGGTGATTACTGTGACAG+AGG 0.510526 1:+61630060 MsG0180003395.01.T01:CDS
GAGCTTGAACCATAGGACTT+CGG 0.510904 1:-61632687 None:intergenic
TGGCGGGAGGAGGTGGAGGA+AGG 0.517276 1:-61630187 None:intergenic
CAAGGTTCTGTAAATGATGC+TGG 0.521943 1:+61630375 MsG0180003395.01.T01:CDS
AGTTGATTGCTGTCCATGCT+GGG 0.538411 1:+61632608 MsG0180003395.01.T01:CDS
TTAAGGTTGATAATTGATGA+AGG 0.545370 1:+61632902 MsG0180003395.01.T01:CDS
GCAAGGGTCGTATGCTGCAT+CGG 0.547959 1:+61629940 MsG0180003395.01.T01:CDS
GCTAAGGGTCGTGTGTATCA+AGG 0.548845 1:+61630357 MsG0180003395.01.T01:CDS
GTCCATGCTGGGTTGGAGAA+AGG 0.556867 1:+61632619 MsG0180003395.01.T01:CDS
TGCCTTTCTCCAACCCAGCA+TGG 0.558579 1:-61632621 None:intergenic
AACTCCGTAGGACTGAAAAG+TGG 0.560340 1:-61630400 None:intergenic
GGAGTTTCTCATGGTTCCGC+CGG 0.570210 1:+61630417 MsG0180003395.01.T01:CDS
GAAGGGTGGTTTACTGGTGA+CGG 0.570581 1:+61630270 MsG0180003395.01.T01:CDS
ACCCGTCGCAGGGTGGCGGG+AGG 0.572448 1:-61630200 None:intergenic
TGTCAGAATCCCATGAAGCA+AGG 0.572451 1:+61629923 MsG0180003395.01.T01:CDS
AGGCGGCGAAGGCGAAGTCA+TGG 0.581598 1:-61630167 None:intergenic
TTGATAATTGATGAAGGTGG+TGG 0.585017 1:+61632908 MsG0180003395.01.T01:CDS
TGCTGCATCGGTGACATCCA+CGG 0.585229 1:+61629952 MsG0180003395.01.T01:CDS
GATTGCTGTCCATGCTGGGT+TGG 0.586278 1:+61632612 MsG0180003395.01.T01:CDS
TCAATTACTTGGCGGGTGTG+AGG 0.589572 1:-61630084 None:intergenic
AGATGCATGTGCAAGGCAGA+AGG 0.592829 1:+61630301 MsG0180003395.01.T01:CDS
GAAAATTCTGACCCGTCGCA+GGG 0.595212 1:-61630210 None:intergenic
AAGTGGTAGAAAGAATGTAT+GGG 0.600330 1:+61632711 MsG0180003395.01.T01:CDS
GTCAGAATCCCATGAAGCAA+GGG 0.600383 1:+61629924 MsG0180003395.01.T01:CDS
TGCATGTGCAAGGCAGAAGG+TGG 0.605884 1:+61630304 MsG0180003395.01.T01:CDS
ACTCCGTAGGACTGAAAAGT+GGG 0.612295 1:-61630399 None:intergenic
TCACGAATAATCTTCATGGA+AGG 0.612634 1:-61632965 None:intergenic
TAGGTGATTACTGTGACAGA+GGG 0.614783 1:+61630061 MsG0180003395.01.T01:CDS
TGTTTATAAAGACATCATCC+TGG 0.618065 1:-61632561 None:intergenic
TATGAGAAGATGCATGTGCA+AGG 0.618350 1:+61630294 MsG0180003395.01.T01:CDS
GTGCAAGGCAGAAGGTGGAG+TGG 0.622956 1:+61630309 MsG0180003395.01.T01:CDS
CCTATGGTTCAAGCTCTAAG+TGG 0.624904 1:+61632694 MsG0180003395.01.T01:CDS
TCAACCTTAAGCCTTCAACA+TGG 0.637711 1:-61632889 None:intergenic
ATGGTGACCACTAACAATGA+TGG 0.654637 1:-61632858 None:intergenic
ATTGTGAAGAGAGAGAAGGG+TGG 0.669873 1:+61630256 MsG0180003395.01.T01:CDS
AATTCTGACCCGTCGCAGGG+TGG 0.692479 1:-61630207 None:intergenic
GAATGTATGGGATATTCCAG+AGG 0.693556 1:+61632723 MsG0180003395.01.T01:CDS
ATGGTCAAATCTCGAAAACA+CGG 0.705782 1:+61629999 MsG0180003395.01.T01:CDS
TGAGCTTGTCAATGAAACCG+TGG 0.713510 1:-61629969 None:intergenic
AGGTTGATAATTGATGAAGG+TGG 0.714328 1:+61632905 MsG0180003395.01.T01:CDS
GGAACCATGAGAAACTCCGT+AGG 0.731973 1:-61630412 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTTTTATATATCATGTTAA+AGG - Chr1:61630749-61630768 None:intergenic 10.0%
!! TATAATCAAAAGATAGAATA+TGG - Chr1:61632243-61632262 None:intergenic 15.0%
!!! ATTTAGATTTTAGATACAAT+TGG - Chr1:61631780-61631799 None:intergenic 15.0%
!! AATAAAACTTTCAAAAACCA+AGG - Chr1:61630519-61630538 None:intergenic 20.0%
!! AATAATTTCGATGCATTATT+TGG - Chr1:61630707-61630726 None:intergenic 20.0%
!! AATCTAAATAACCAAACAAA+AGG + Chr1:61631791-61631810 MsG0180003395.01.T01:intron 20.0%
!! ACTAAATTTAATGTTGCTAA+GGG + Chr1:61630342-61630361 MsG0180003395.01.T01:CDS 20.0%
!! ATTGTTAATTTAATCGACAA+TGG - Chr1:61632514-61632533 None:intergenic 20.0%
!! CTATAAGAAAATCAATTACT+TGG - Chr1:61630098-61630117 None:intergenic 20.0%
!! CTTAAGAAATATGATAGAAT+AGG + Chr1:61631190-61631209 MsG0180003395.01.T01:intron 20.0%
!! GTTAAAGGAAAATACTTATA+TGG - Chr1:61630734-61630753 None:intergenic 20.0%
!! TAACAAATTTCATTATGCTT+GGG + Chr1:61630573-61630592 MsG0180003395.01.T01:intron 20.0%
!! TATCAAACATATACTTAAGA+CGG - Chr1:61630464-61630483 None:intergenic 20.0%
!!! TAAGAAAACTGTTATTTCTT+TGG - Chr1:61631174-61631193 None:intergenic 20.0%
! AAATCCATATGCATTGTATT+TGG - Chr1:61632303-61632322 None:intergenic 25.0%
! AATCCATATGCATTGTATTT+GGG - Chr1:61632302-61632321 None:intergenic 25.0%
! ACATATTGCAGATAAATCAA+CGG - Chr1:61631691-61631710 None:intergenic 25.0%
! AGATAAATTAGTACTCTCTA+AGG + Chr1:61632333-61632352 MsG0180003395.01.T01:intron 25.0%
! ATATGAGTATAGAAACAAAG+AGG - Chr1:61632465-61632484 None:intergenic 25.0%
! CATAATTATACGAATTACCT+TGG + Chr1:61630499-61630518 MsG0180003395.01.T01:intron 25.0%
! CATACAAAAAATCATTGTGA+GGG + Chr1:61631540-61631559 MsG0180003395.01.T01:intron 25.0%
! CTAACAAATTTCATTATGCT+TGG + Chr1:61630572-61630591 MsG0180003395.01.T01:intron 25.0%
! GACTAAATTTAATGTTGCTA+AGG + Chr1:61630341-61630360 MsG0180003395.01.T01:CDS 25.0%
! GAGTACTAATTTATCTATTG+CGG - Chr1:61632329-61632348 None:intergenic 25.0%
! GTCTTTATAAACATTGATGA+TGG + Chr1:61632571-61632590 MsG0180003395.01.T01:CDS 25.0%
! TAAGAAAATCAATTACTTGG+CGG - Chr1:61630095-61630114 None:intergenic 25.0%
! TAAGAAATTTCTTGCGAATT+TGG + Chr1:61632403-61632422 MsG0180003395.01.T01:intron 25.0%
! TATATAATGAGAAGCAATCT+TGG - Chr1:61630843-61630862 None:intergenic 25.0%
! TTCTTCAAATTCGAATAGAA+TGG + Chr1:61632798-61632817 MsG0180003395.01.T01:intron 25.0%
!! ATTTTGGATTATGTTTTACC+AGG + Chr1:61632543-61632562 MsG0180003395.01.T01:CDS 25.0%
!!! AGCTTTAAATTATGTGTGAA+TGG + Chr1:61632015-61632034 MsG0180003395.01.T01:intron 25.0%
!!! AGGAAAACATGTTTTTGATT+CGG - Chr1:61630138-61630157 None:intergenic 25.0%
!!! GGAAGATTAAGTGTTTTATT+TGG - Chr1:61630933-61630952 None:intergenic 25.0%
!!! TAACAATTCTGAAAGCATTT+TGG + Chr1:61632527-61632546 MsG0180003395.01.T01:intron 25.0%
!!! TAAGTGTTTTATTTGGTTAG+TGG - Chr1:61630926-61630945 None:intergenic 25.0%
!!! TTAAGGTTGATAATTGATGA+AGG + Chr1:61632902-61632921 MsG0180003395.01.T01:CDS 25.0%
!!! TTACAAAAGAGAGTTTTAAG+AGG + Chr1:61630657-61630676 MsG0180003395.01.T01:intron 25.0%
AAAAAATCATTGTGAGGGTT+AGG + Chr1:61631545-61631564 MsG0180003395.01.T01:intron 30.0%
AAAAACTGCTCAAGAACTTA+AGG + Chr1:61631260-61631279 MsG0180003395.01.T01:intron 30.0%
AAAAATCATTGTGAGGGTTA+GGG + Chr1:61631546-61631565 MsG0180003395.01.T01:intron 30.0%
AAAACTGCTCAAGAACTTAA+GGG + Chr1:61631261-61631280 MsG0180003395.01.T01:intron 30.0%
AAGAAAATCAATTACTTGGC+GGG - Chr1:61630094-61630113 None:intergenic 30.0%
AAGTGGTAGAAAGAATGTAT+GGG + Chr1:61632711-61632730 MsG0180003395.01.T01:CDS 30.0%
AATTCAAACCTCACAAAATC+TGG + Chr1:61630862-61630881 MsG0180003395.01.T01:intron 30.0%
AATTTCTTGCGAATTTGGTT+TGG + Chr1:61632408-61632427 MsG0180003395.01.T01:intron 30.0%
ACACAATTGTCTTATGGAAT+AGG + Chr1:61631099-61631118 MsG0180003395.01.T01:intron 30.0%
ACCACTACAATCTCAATATT+AGG + Chr1:61630899-61630918 MsG0180003395.01.T01:intron 30.0%
AGAAATTTCTTATGCTCATC+AGG - Chr1:61632395-61632414 None:intergenic 30.0%
ATCACCTAAGAAGATGATAA+TGG - Chr1:61630049-61630068 None:intergenic 30.0%
ATTCCATAAGACAATTGTGT+GGG - Chr1:61631099-61631118 None:intergenic 30.0%
ATTCGTGATACAGATCTATT+AGG + Chr1:61632980-61632999 MsG0180003395.01.T01:CDS 30.0%
CATCTAATTTCACTATGTAC+TGG + Chr1:61631882-61631901 MsG0180003395.01.T01:intron 30.0%
CGGAAGAGTAAAATAAATCT+TGG - Chr1:61631671-61631690 None:intergenic 30.0%
CTTCCTTACTTTAGTGAAAA+TGG + Chr1:61630948-61630967 MsG0180003395.01.T01:intron 30.0%
GAATGAATGCTTGACAATAT+AGG + Chr1:61631016-61631035 MsG0180003395.01.T01:intron 30.0%
GAATGGCATAGTTGAATTAT+AGG + Chr1:61632032-61632051 MsG0180003395.01.T01:intron 30.0%
GCATACAAAAAATCATTGTG+AGG + Chr1:61631539-61631558 MsG0180003395.01.T01:intron 30.0%
TAACCCAAATACAATGCATA+TGG + Chr1:61632296-61632315 MsG0180003395.01.T01:intron 30.0%
TAAGTGGTAGAAAGAATGTA+TGG + Chr1:61632710-61632729 MsG0180003395.01.T01:CDS 30.0%
TAGATGACAGATAACCATAA+GGG - Chr1:61631868-61631887 None:intergenic 30.0%
TATTCCATAAGACAATTGTG+TGG - Chr1:61631100-61631119 None:intergenic 30.0%
TCCTAATATTGAGATTGTAG+TGG - Chr1:61630903-61630922 None:intergenic 30.0%
TGTATCACGAATAATCTTCA+TGG - Chr1:61632972-61632991 None:intergenic 30.0%
TGTTTATAAAGACATCATCC+TGG - Chr1:61632564-61632583 None:intergenic 30.0%
TTAGATGACAGATAACCATA+AGG - Chr1:61631869-61631888 None:intergenic 30.0%
TTAGTGAAAATGGCTTCTAA+TGG + Chr1:61630958-61630977 MsG0180003395.01.T01:intron 30.0%
! ATGCATTGTATTTGGGTTAA+TGG - Chr1:61632295-61632314 None:intergenic 30.0%
! ATGGCTAATTGTTTTCTTGT+AGG + Chr1:61632817-61632836 MsG0180003395.01.T01:intron 30.0%
! ATTTCTTGCGAATTTGGTTT+GGG + Chr1:61632409-61632428 MsG0180003395.01.T01:intron 30.0%
! CTGTTTTCGTAAGTTATCAT+AGG - Chr1:61632194-61632213 None:intergenic 30.0%
! TGAATAGAGTATTCTCTCTT+AGG - Chr1:61631241-61631260 None:intergenic 30.0%
! TGCATTGTATTTGGGTTAAT+GGG - Chr1:61632294-61632313 None:intergenic 30.0%
!! AAGCCATTTTCACTAAAGTA+AGG - Chr1:61630954-61630973 None:intergenic 30.0%
!! AGACTAGGTTGATTAATTCT+AGG - Chr1:61631382-61631401 None:intergenic 30.0%
!! ATAACCAAACAAAAGGTGTT+AGG + Chr1:61631798-61631817 MsG0180003395.01.T01:intron 30.0%
!! CATATTTCTGATAGTTTTGC+TGG + Chr1:61633013-61633032 MsG0180003395.01.T01:three_prime_UTR 30.0%
!! GACTAGGTTGATTAATTCTA+GGG - Chr1:61631381-61631400 None:intergenic 30.0%
!! GGAAAACATGTTTTTGATTC+GGG - Chr1:61630137-61630156 None:intergenic 30.0%
!!! CACAATGATTTTTTGTATGC+AGG - Chr1:61631539-61631558 None:intergenic 30.0%
AAATTCTACTAGTGCAGCTT+TGG - Chr1:61631147-61631166 None:intergenic 35.0%
AACGAATCCAATCCTTTGTT+TGG - Chr1:61631727-61631746 None:intergenic 35.0%
ACAGCCATTATCATCTTCTT+AGG + Chr1:61630042-61630061 MsG0180003395.01.T01:CDS 35.0%
AGATGACAGATAACCATAAG+GGG - Chr1:61631867-61631886 None:intergenic 35.0%
AGTTCAACCATCATTGTTAG+TGG + Chr1:61632851-61632870 MsG0180003395.01.T01:CDS 35.0%
ATACCCACACAATTGTCTTA+TGG + Chr1:61631093-61631112 MsG0180003395.01.T01:intron 35.0%
ATGCATAAGAAGCCAAACAA+AGG + Chr1:61631712-61631731 MsG0180003395.01.T01:intron 35.0%
ATGGATGCTTGATTCATTCA+CGG + Chr1:61631619-61631638 MsG0180003395.01.T01:intron 35.0%
ATGGTCAAATCTCGAAAACA+CGG + Chr1:61629999-61630018 MsG0180003395.01.T01:CDS 35.0%
CAATTCATTGCATGCAGATT+TGG + Chr1:61632358-61632377 MsG0180003395.01.T01:intron 35.0%
CAGGTTACTTAGAAATGAAG+AGG - Chr1:61631520-61631539 None:intergenic 35.0%
GGAAGAATAATTGCAGCTAT+TGG - Chr1:61632947-61632966 None:intergenic 35.0%
GTTACTTAGAAATGAAGAGG+CGG - Chr1:61631517-61631536 None:intergenic 35.0%
TAAGAAGCCAAACAAAGGAT+TGG + Chr1:61631717-61631736 MsG0180003395.01.T01:intron 35.0%
TACTTAAGACGGCTATATGT+CGG - Chr1:61630453-61630472 None:intergenic 35.0%
TCACGAATAATCTTCATGGA+AGG - Chr1:61632968-61632987 None:intergenic 35.0%
TTGACAAGCTCAAAAACCTA+TGG + Chr1:61629980-61629999 MsG0180003395.01.T01:CDS 35.0%
! AGAATAGGATTTTCTCCTCA+AGG + Chr1:61631205-61631224 MsG0180003395.01.T01:intron 35.0%
! ATTGCATGCAGATTTGGTTA+AGG + Chr1:61632364-61632383 MsG0180003395.01.T01:intron 35.0%
! CAAAAACATGTTTTCCTAGC+TGG + Chr1:61630141-61630160 MsG0180003395.01.T01:CDS 35.0%
! GAATAGGATTTTCTCCTCAA+GGG + Chr1:61631206-61631225 MsG0180003395.01.T01:intron 35.0%
! GATTCTGACATTGTTGTTGT+TGG - Chr1:61629913-61629932 None:intergenic 35.0%
! TAGAGTATTCTCTCTTAGGA+AGG - Chr1:61631237-61631256 None:intergenic 35.0%
! TTGATAATTGATGAAGGTGG+TGG + Chr1:61632908-61632927 MsG0180003395.01.T01:CDS 35.0%
!! AGGTTGATAATTGATGAAGG+TGG + Chr1:61632905-61632924 MsG0180003395.01.T01:CDS 35.0%
!! ATGGCTGTTTTGAATTGTGA+TGG - Chr1:61630030-61630049 None:intergenic 35.0%
!! TGTTTTCGAGATTTGACCAT+AGG - Chr1:61629999-61630018 None:intergenic 35.0%
!!! ATGTTTTTGATTCGGGTAAC+GGG - Chr1:61630130-61630149 None:intergenic 35.0%
!!! CATGTTTTTGATTCGGGTAA+CGG - Chr1:61630131-61630150 None:intergenic 35.0%
AACTCCATGTTGAAGGCTTA+AGG + Chr1:61632885-61632904 MsG0180003395.01.T01:CDS 40.0%
ACAAAGGATTGGATTCGTTC+TGG + Chr1:61631728-61631747 MsG0180003395.01.T01:intron 40.0%
ACTTAAGACGGCTATATGTC+GGG - Chr1:61630452-61630471 None:intergenic 40.0%
AGCATTGTGAAGAGAGAGAA+GGG + Chr1:61630253-61630272 MsG0180003395.01.T01:CDS 40.0%
ATGAGAAGCAATCTTGGTCA+TGG - Chr1:61630837-61630856 None:intergenic 40.0%
ATGGTGACCACTAACAATGA+TGG - Chr1:61632861-61632880 None:intergenic 40.0%
CAAAAGGACTACAAGAGCAA+AGG + Chr1:61631466-61631485 MsG0180003395.01.T01:intron 40.0%
CAAGGTTCTGTAAATGATGC+TGG + Chr1:61630375-61630394 MsG0180003395.01.T01:CDS 40.0%
CATGCAAAACTCCATGTTGA+AGG + Chr1:61632878-61632897 MsG0180003395.01.T01:CDS 40.0%
CGTTCTTGTATGCTTTGGTT+TGG - Chr1:61629888-61629907 None:intergenic 40.0%
GAATGTATGGGATATTCCAG+AGG + Chr1:61632723-61632742 MsG0180003395.01.T01:CDS 40.0%
GTGTCTCAGATGGTCTAAAT+AGG - Chr1:61631069-61631088 None:intergenic 40.0%
TAGGTGATTACTGTGACAGA+GGG + Chr1:61630061-61630080 MsG0180003395.01.T01:CDS 40.0%
TATGAGAAGATGCATGTGCA+AGG + Chr1:61630294-61630313 MsG0180003395.01.T01:CDS 40.0%
TCAACCTTAAGCCTTCAACA+TGG - Chr1:61632892-61632911 None:intergenic 40.0%
TGGTTCGTTCTTGTATGCTT+TGG - Chr1:61629893-61629912 None:intergenic 40.0%
TGTGCAAAGACTCAAGTTCA+CGG + Chr1:61631836-61631855 MsG0180003395.01.T01:intron 40.0%
TTAGGTGATTACTGTGACAG+AGG + Chr1:61630060-61630079 MsG0180003395.01.T01:CDS 40.0%
TTGACCTTAGCTCAAACTCA+CGG - Chr1:61631417-61631436 None:intergenic 40.0%
! AAGCATTGTGAAGAGAGAGA+AGG + Chr1:61630252-61630271 MsG0180003395.01.T01:CDS 40.0%
! AGTGCCTAACACCTTTTGTT+TGG - Chr1:61631805-61631824 None:intergenic 40.0%
! CAGCTTTGGCAAGACTAAAA+AGG - Chr1:61631133-61631152 None:intergenic 40.0%
! CATTCACGGTTTTGCAATGA+GGG + Chr1:61631633-61631652 MsG0180003395.01.T01:intron 40.0%
! CTACTTGACCAGATTTTGTG+AGG - Chr1:61630873-61630892 None:intergenic 40.0%
! CTTCAACATGGAGTTTTGCA+TGG - Chr1:61632880-61632899 None:intergenic 40.0%
! GTTTTGCAATGAGGGCAATA+AGG + Chr1:61631641-61631660 MsG0180003395.01.T01:intron 40.0%
! TCATTCACGGTTTTGCAATG+AGG + Chr1:61631632-61631651 MsG0180003395.01.T01:intron 40.0%
! TTGCTCTTGTAGTCCTTTTG+TGG - Chr1:61631466-61631485 None:intergenic 40.0%
!! GGGTATAAAAGTGTCTCAGA+TGG - Chr1:61631079-61631098 None:intergenic 40.0%
!! TTTGGTTTGGGTTCATGAAG+AGG + Chr1:61632421-61632440 MsG0180003395.01.T01:intron 40.0%
AACTCCGTAGGACTGAAAAG+TGG - Chr1:61630403-61630422 None:intergenic 45.0%
AAGAAGCAAGCTATCACCTC+TGG - Chr1:61632742-61632761 None:intergenic 45.0%
AAGTCTCCCGGAGGATATTT+CGG - Chr1:61631302-61631321 None:intergenic 45.0%
ACACATACCATTGCCAACAC+AGG - Chr1:61631991-61632010 None:intergenic 45.0%
ACTCCGTAGGACTGAAAAGT+GGG - Chr1:61630402-61630421 None:intergenic 45.0%
AGAGATTGCCCTCAGGAAAA+GGG - Chr1:61631593-61631612 None:intergenic 45.0%
AGCAATCTTGGTCATGGTCA+TGG - Chr1:61630831-61630850 None:intergenic 45.0%
AGCTCAAACTCACGGATACA+TGG - Chr1:61631409-61631428 None:intergenic 45.0%
AGCTTGAACCATAGGACTTC+GGG - Chr1:61632689-61632708 None:intergenic 45.0%
AGTTGATTGCTGTCCATGCT+GGG + Chr1:61632608-61632627 MsG0180003395.01.T01:CDS 45.0%
ATATGTCGGGGGATACTTAC+CGG - Chr1:61630439-61630458 None:intergenic 45.0%
ATTGTGAAGAGAGAGAAGGG+TGG + Chr1:61630256-61630275 MsG0180003395.01.T01:CDS 45.0%
CCACTTAGAGCTTGAACCAT+AGG - Chr1:61632697-61632716 None:intergenic 45.0%
CTTAAGACGGCTATATGTCG+GGG - Chr1:61630451-61630470 None:intergenic 45.0%
CTTATCCCCGAAATATCCTC+CGG + Chr1:61631292-61631311 MsG0180003395.01.T01:intron 45.0%
GAGCTTGAACCATAGGACTT+CGG - Chr1:61632690-61632709 None:intergenic 45.0%
GAGGGCAATCTCTCAAAACA+TGG + Chr1:61631600-61631619 MsG0180003395.01.T01:intron 45.0%
GTATCCGTGAGTTTGAGCTA+AGG + Chr1:61631410-61631429 MsG0180003395.01.T01:intron 45.0%
GTCAGAATCCCATGAAGCAA+GGG + Chr1:61629924-61629943 MsG0180003395.01.T01:CDS 45.0%
TATGCAGCTTCTACCTGTGT+TGG + Chr1:61631975-61631994 MsG0180003395.01.T01:intron 45.0%
TGAGCTTGTCAATGAAACCG+TGG - Chr1:61629972-61629991 None:intergenic 45.0%
TGTCAGAATCCCATGAAGCA+AGG + Chr1:61629923-61629942 MsG0180003395.01.T01:CDS 45.0%
TTAAGACGGCTATATGTCGG+GGG - Chr1:61630450-61630469 None:intergenic 45.0%
TTAGGAAGGAGAAACCCTTG+AGG - Chr1:61631223-61631242 None:intergenic 45.0%
TTATCCCCGAAATATCCTCC+GGG + Chr1:61631293-61631312 MsG0180003395.01.T01:intron 45.0%
! AAGTTGATTGCTGTCCATGC+TGG + Chr1:61632607-61632626 MsG0180003395.01.T01:CDS 45.0%
! ACGGGTCAGAATTTTCTGAG+GGG + Chr1:61630217-61630236 MsG0180003395.01.T01:CDS 45.0%
! GACGGGTCAGAATTTTCTGA+GGG + Chr1:61630216-61630235 MsG0180003395.01.T01:CDS 45.0%
!! AGAATTTTCTGAGGGGTGGA+TGG + Chr1:61630224-61630243 MsG0180003395.01.T01:CDS 45.0%
!! CCTATGGTTCAAGCTCTAAG+TGG + Chr1:61632694-61632713 MsG0180003395.01.T01:CDS 45.0%
!! TCCTTTTGTGGCTGAGTTGA+GGG - Chr1:61631454-61631473 None:intergenic 45.0%
!!! TGTTTTGAGAGATTGCCCTC+AGG - Chr1:61631600-61631619 None:intergenic 45.0%
!!! TTTTGATTCGGGTAACGGGT+TGG - Chr1:61630126-61630145 None:intergenic 45.0%
AGAAAATTCTGACCCGTCGC+AGG - Chr1:61630214-61630233 None:intergenic 50.0%
AGATGCATGTGCAAGGCAGA+AGG + Chr1:61630301-61630320 MsG0180003395.01.T01:CDS 50.0%
AGCATACGACCCTTGCTTCA+TGG - Chr1:61629936-61629955 None:intergenic 50.0%
AGTCTCCCGGAGGATATTTC+GGG - Chr1:61631301-61631320 None:intergenic 50.0%
CAGTCCTACGGAGTTTCTCA+TGG + Chr1:61630408-61630427 MsG0180003395.01.T01:CDS 50.0%
CGAAGTCATGGTTTCCAGCT+AGG - Chr1:61630158-61630177 None:intergenic 50.0%
CGGATACATGGCAGTAGACT+AGG - Chr1:61631397-61631416 None:intergenic 50.0%
GAAAATTCTGACCCGTCGCA+GGG - Chr1:61630213-61630232 None:intergenic 50.0%
GAAGAGGCGGTCTTGATATG+TGG - Chr1:61631504-61631523 None:intergenic 50.0%
GAAGGGTGGTTTACTGGTGA+CGG + Chr1:61630270-61630289 MsG0180003395.01.T01:CDS 50.0%
GAGAGAGAAGGGTGGTTTAC+TGG + Chr1:61630264-61630283 MsG0180003395.01.T01:CDS 50.0%
GAGAGATTGCCCTCAGGAAA+AGG - Chr1:61631594-61631613 None:intergenic 50.0%
GCATACGACCCTTGCTTCAT+GGG - Chr1:61629935-61629954 None:intergenic 50.0%
GCTAAGGGTCGTGTGTATCA+AGG + Chr1:61630357-61630376 MsG0180003395.01.T01:CDS 50.0%
GGAACCATGAGAAACTCCGT+AGG - Chr1:61630415-61630434 None:intergenic 50.0%
GTTCACGGTATAGCCCCTTA+TGG + Chr1:61631851-61631870 MsG0180003395.01.T01:intron 50.0%
TCGAGATACCCGAAGTCCTA+TGG + Chr1:61632678-61632697 MsG0180003395.01.T01:CDS 50.0%
! CGACGGGTCAGAATTTTCTG+AGG + Chr1:61630215-61630234 MsG0180003395.01.T01:CDS 50.0%
! GCTTCTACCTGTGTTGGCAA+TGG + Chr1:61631981-61632000 MsG0180003395.01.T01:intron 50.0%
! GGTTTGGGTTCATGAAGAGG+TGG + Chr1:61632424-61632443 MsG0180003395.01.T01:intron 50.0%
! GTCCTTTTGTGGCTGAGTTG+AGG - Chr1:61631455-61631474 None:intergenic 50.0%
! TCAATTACTTGGCGGGTGTG+AGG - Chr1:61630087-61630106 None:intergenic 50.0%
!! CCTTTTGTGGCTGAGTTGAG+GGG - Chr1:61631453-61631472 None:intergenic 50.0%
!! GGTCAGAATTTTCTGAGGGG+TGG + Chr1:61630220-61630239 MsG0180003395.01.T01:CDS 50.0%
!! GGTCCCACTTTTCAGTCCTA+CGG + Chr1:61630396-61630415 MsG0180003395.01.T01:CDS 50.0%
!!! GAGTTGAGGGGAGTTTTGGA+AGG - Chr1:61631441-61631460 None:intergenic 50.0%
CCCCTCAACTCAGCCACAAA+AGG + Chr1:61631450-61631469 MsG0180003395.01.T01:intron 55.0%
GATTGCTGTCCATGCTGGGT+TGG + Chr1:61632612-61632631 MsG0180003395.01.T01:CDS 55.0%
GCAAGGGTCGTATGCTGCAT+CGG + Chr1:61629940-61629959 MsG0180003395.01.T01:CDS 55.0%
GCACACAGGAGAAAGTCTCC+CGG - Chr1:61631314-61631333 None:intergenic 55.0%
GGAGTTTCTCATGGTTCCGC+CGG + Chr1:61630417-61630436 MsG0180003395.01.T01:CDS 55.0%
GTCTCCCGGAGGATATTTCG+GGG - Chr1:61631300-61631319 None:intergenic 55.0%
TGCATGTGCAAGGCAGAAGG+TGG + Chr1:61630304-61630323 MsG0180003395.01.T01:CDS 55.0%
TGCCTTTCTCCAACCCAGCA+TGG - Chr1:61632624-61632643 None:intergenic 55.0%
! GCCACACACCCTTTTCCTGA+GGG + Chr1:61631582-61631601 MsG0180003395.01.T01:intron 55.0%
! GTCCATGCTGGGTTGGAGAA+AGG + Chr1:61632619-61632638 MsG0180003395.01.T01:CDS 55.0%
! TGCTGCATCGGTGACATCCA+CGG + Chr1:61629952-61629971 MsG0180003395.01.T01:CDS 55.0%
!! GGCTGAGTTGAGGGGAGTTT+TGG - Chr1:61631445-61631464 None:intergenic 55.0%
AATTCTGACCCGTCGCAGGG+TGG - Chr1:61630210-61630229 None:intergenic 60.0%
CACAGGAGAAAGTCTCCCGG+AGG - Chr1:61631311-61631330 None:intergenic 60.0%
GGGTTAGGGTGAACAAGCCG+CGG + Chr1:61631560-61631579 MsG0180003395.01.T01:intron 60.0%
GTGCAAGGCAGAAGGTGGAG+TGG + Chr1:61630309-61630328 MsG0180003395.01.T01:CDS 60.0%
TGTCGGGGGATACTTACCGG+CGG - Chr1:61630436-61630455 None:intergenic 60.0%
! AGGAAAAGGGTGTGTGGCCG+CGG - Chr1:61631580-61631599 None:intergenic 60.0%
! GCCCTCAGGAAAAGGGTGTG+TGG - Chr1:61631586-61631605 None:intergenic 60.0%
! GGCCACACACCCTTTTCCTG+AGG + Chr1:61631581-61631600 MsG0180003395.01.T01:intron 60.0%
AGGCGGCGAAGGCGAAGTCA+TGG - Chr1:61630170-61630189 None:intergenic 65.0%
ATATGCCGCGATCCGCGCGA+GGG - Chr1:61631340-61631359 None:intergenic 65.0%
GCGCGGATCGCGGCATATAC+AGG + Chr1:61631342-61631361 MsG0180003395.01.T01:intron 65.0%
TATATGCCGCGATCCGCGCG+AGG - Chr1:61631341-61631360 None:intergenic 65.0%
CGGGAGGAGGTGGAGGAAGG+CGG - Chr1:61630187-61630206 None:intergenic 70.0%
GAGGTGGAGGAAGGCGGCGA+AGG - Chr1:61630181-61630200 None:intergenic 70.0%
TCTGACCCGTCGCAGGGTGG+CGG - Chr1:61630207-61630226 None:intergenic 70.0%
TGGCGGGAGGAGGTGGAGGA+AGG - Chr1:61630190-61630209 None:intergenic 70.0%
CCTGTGTGCCCACCCTCGCG+CGG + Chr1:61631325-61631344 MsG0180003395.01.T01:intron 75.0%
CTCCTCCCGCCACCCTGCGA+CGG + Chr1:61630198-61630217 MsG0180003395.01.T01:CDS 75.0%
CTGACCCGTCGCAGGGTGGC+GGG - Chr1:61630206-61630225 None:intergenic 75.0%
TCCTCCCGCCACCCTGCGAC+GGG + Chr1:61630199-61630218 MsG0180003395.01.T01:CDS 75.0%
!! AGGGTGGCGGGAGGAGGTGG+AGG - Chr1:61630194-61630213 None:intergenic 75.0%
! CCGCGCGAGGGTGGGCACAC+AGG - Chr1:61631328-61631347 None:intergenic 80.0%
! GCCCACCCTCGCGCGGATCG+CGG + Chr1:61631332-61631351 MsG0180003395.01.T01:intron 80.0%
! GCCGCGATCCGCGCGAGGGT+GGG - Chr1:61631336-61631355 None:intergenic 80.0%
! TGCCGCGATCCGCGCGAGGG+TGG - Chr1:61631337-61631356 None:intergenic 80.0%
!!! ACCCGTCGCAGGGTGGCGGG+AGG - Chr1:61630203-61630222 None:intergenic 80.0%
!!! CGCAGGGTGGCGGGAGGAGG+TGG - Chr1:61630197-61630216 None:intergenic 80.0%
!!! CGTCGCAGGGTGGCGGGAGG+AGG - Chr1:61630200-61630219 None:intergenic 80.0%
Chromosome Type Strat End Strand Name
Chr1 gene 61629881 61633059 61629881 ID=MsG0180003395.01;Name=MsG0180003395.01
Chr1 mRNA 61629881 61633059 61629881 ID=MsG0180003395.01.T01;Parent=MsG0180003395.01;Name=MsG0180003395.01.T01;_AED=0.42;_eAED=0.42;_QI=41|0.5|0.66|1|0.5|0.33|3|50|296
Chr1 exon 61629881 61630438 61629881 ID=MsG0180003395.01.T01:exon:49654;Parent=MsG0180003395.01.T01
Chr1 exon 61632542 61632744 61632542 ID=MsG0180003395.01.T01:exon:49655;Parent=MsG0180003395.01.T01
Chr1 exon 61632839 61633059 61632839 ID=MsG0180003395.01.T01:exon:49656;Parent=MsG0180003395.01.T01
Chr1 five_prime_UTR 61629881 61629921 61629881 ID=MsG0180003395.01.T01:five_prime_utr;Parent=MsG0180003395.01.T01
Chr1 CDS 61629922 61630438 61629922 ID=MsG0180003395.01.T01:cds;Parent=MsG0180003395.01.T01
Chr1 CDS 61632542 61632744 61632542 ID=MsG0180003395.01.T01:cds;Parent=MsG0180003395.01.T01
Chr1 CDS 61632839 61633009 61632839 ID=MsG0180003395.01.T01:cds;Parent=MsG0180003395.01.T01
Chr1 three_prime_UTR 61633010 61633059 61633010 ID=MsG0180003395.01.T01:three_prime_utr;Parent=MsG0180003395.01.T01
Gene Sequence

>MsG0180003395.01.T01

ATGTCAGAATCCCATGAAGCAAGGGTCGTATGCTGCATCGGTGACATCCACGGTTTCATTGACAAGCTCAAAAACCTATGGTCAAATCTCGAAAACACGGTCGAACCATCACAATTCAAAACAGCCATTATCATCTTCTTAGGTGATTACTGTGACAGAGGGCCTCACACCCGCCAAGTAATTGATTTTCTTATAGCATTACCAACCCGTTACCCGAATCAAAAACATGTTTTCCTAGCTGGAAACCATGACTTCGCCTTCGCCGCCTTCCTCCACCTCCTCCCGCCACCCTGCGACGGGTCAGAATTTTCTGAGGGGTGGATGGAGTTTAAGCATTGTGAAGAGAGAGAAGGGTGGTTTACTGGTGACGGATATGAGAAGATGCATGTGCAAGGCAGAAGGTGGAGTGGAAGTATAAAGACTAAATTTAATGTTGCTAAGGGTCGTGTGTATCAAGGTTCTGTAAATGATGCTGGTCCCACTTTTCAGTCCTACGGAGTTTCTCATGGTTCCGCCGCATTTTGGATTATGTTTTACCAGGATGATGTCTTTATAAACATTGATGATGGAGTTAAGTGCTACAAGTTGATTGCTGTCCATGCTGGGTTGGAGAAAGGCATAGATGTGAAAGAACAGATGAAAAATTTGAAAGCTCGAGATACCCGAAGTCCTATGGTTCAAGCTCTAAGTGGTAGAAAGAATGTATGGGATATTCCAGAGGAACTAAGTGCAAGTTCAACCATCATTGTTAGTGGTCACCATGCAAAACTCCATGTTGAAGGCTTAAGGTTGATAATTGATGAAGGTGGTGGACACAAAGATAAACCAATAGCTGCAATTATTCTTCCTTCCATGAAGATTATTCGTGATACAGATCTATTAGGAAAATAG
Protein sequence

>MsG0180003395.01.T01

MSESHEARVVCCIGDIHGFIDKLKNLWSNLENTVEPSQFKTAIIIFLGDYCDRGPHTRQVIDFLIALPTRYPNQKHVFLAGNHDFAFAAFLHLLPPPCDGSEFSEGWMEFKHCEEREGWFTGDGYEKMHVQGRRWSGSIKTKFNVAKGRVYQGSVNDAGPTFQSYGVSHGSAAFWIMFYQDDVFINIDDGVKCYKLIAVHAGLEKGIDVKEQMKNLKARDTRSPMVQALSGRKNVWDIPEELSASSTIIVSGHHAKLHVEGLRLIIDEGGGHKDKPIAAIILPSMKIIRDTDLLGK*