Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180003528.01.T01 | XP_013468168.1 | 75 | 96 | 4 | 1 | 1 | 96 | 1 | 76 | 1.55E-38 | 134 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180003528.01.T01 | Q2HIM5 | 55.208 | 96 | 23 | 1 | 1 | 96 | 1 | 76 | 2.19E-26 | 96.7 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180003528.01.T01 | I3SW37 | 75.000 | 96 | 4 | 1 | 1 | 96 | 1 | 76 | 7.39e-39 | 134 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180003528.01 | MsG0780040721.01 | 0.812278 | 4.595386e-51 | 2.909403e-48 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180003528.01.T01 | MTR_1g063370 | 75.000 | 96 | 4 | 1 | 1 | 96 | 1 | 76 | 1.87e-42 | 134 |
| MsG0180003528.01.T01 | MTR_7g101880 | 55.914 | 93 | 21 | 1 | 4 | 96 | 5 | 77 | 3.36e-27 | 95.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180003528.01.T01 | AT5G03345 | 55.208 | 96 | 23 | 1 | 1 | 96 | 1 | 76 | 2.23e-27 | 96.7 |
| MsG0180003528.01.T01 | AT5G03345 | 56.250 | 96 | 22 | 2 | 1 | 96 | 1 | 76 | 4.43e-26 | 93.2 |
Find 25 sgRNAs with CRISPR-Local
Find 40 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATGCTTGATTATATGAAAAT+TGG | 0.174922 | 1:-63680504 | MsG0180003528.01.T01:CDS |
| TACCTGTTCTCATCAGAATC+TGG | 0.307650 | 1:+63680256 | None:intergenic |
| TGGGCTGCTTTGACTGTGCC+AGG | 0.322696 | 1:-63680298 | MsG0180003528.01.T01:CDS |
| ATGGGTTTAGGTTTCACTAT+TGG | 0.328659 | 1:-63680652 | MsG0180003528.01.T01:CDS |
| TAATTGCAATTAGCAAGTTT+TGG | 0.344456 | 1:-63680770 | None:intergenic |
| ATTATTATTATTACATTGAA+TGG | 0.349745 | 1:+63680588 | None:intergenic |
| GCACCGAAAACAATGGGTTT+AGG | 0.363317 | 1:-63680664 | MsG0180003528.01.T01:exon |
| GTTGTGATTGAACTTACTTT+AGG | 0.370573 | 1:-63680337 | MsG0180003528.01.T01:CDS |
| TATGAAAATTGGGTTTGATT+TGG | 0.388932 | 1:-63680493 | MsG0180003528.01.T01:CDS |
| TGCTTGATTATATGAAAATT+GGG | 0.405422 | 1:-63680503 | MsG0180003528.01.T01:CDS |
| TCCCAGATTCTGATGAGAAC+AGG | 0.458040 | 1:-63680258 | MsG0180003528.01.T01:CDS |
| GGTTTGATTTGGTTGCAGAT+AGG | 0.470613 | 1:-63680482 | MsG0180003528.01.T01:CDS |
| ACCTGTTCTCATCAGAATCT+GGG | 0.480405 | 1:+63680257 | None:intergenic |
| GGTTTCACTATTGGATTCAT+CGG | 0.491819 | 1:-63680643 | MsG0180003528.01.T01:CDS |
| AAGATCGCACCGAAAACAAT+GGG | 0.531401 | 1:-63680670 | MsG0180003528.01.T01:exon |
| GGGCTGCTTTGACTGTGCCA+GGG | 0.544376 | 1:-63680297 | MsG0180003528.01.T01:CDS |
| TGAATAAGTAATTAGCTCCA+AGG | 0.554658 | 1:+63680742 | None:intergenic |
| GGTTTCAGAAGGACAAAGGT+CGG | 0.569182 | 1:+63680697 | None:intergenic |
| AAAGATCGCACCGAAAACAA+TGG | 0.580112 | 1:-63680671 | MsG0180003528.01.T01:exon |
| GAATAAGTAATTAGCTCCAA+GGG | 0.589648 | 1:+63680743 | None:intergenic |
| GGAGATTTCGATGAGGAACA+CGG | 0.603640 | 1:+63680718 | None:intergenic |
| GACTTTGTTGAAGATTACAG+AGG | 0.686086 | 1:-63680460 | MsG0180003528.01.T01:CDS |
| AATAAGTAATTAGCTCCAAG+GGG | 0.709038 | 1:+63680744 | None:intergenic |
| TGTTCTCATCAGAATCTGGG+AGG | 0.722575 | 1:+63680260 | None:intergenic |
| AAAGGTCGGAGATTTCGATG+AGG | 0.729037 | 1:+63680711 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATTATTATTATTACATTGAA+TGG | + | Chr1:63680426-63680445 | None:intergenic | 10.0% |
| !!! | GAATTTATTTTACAGTAAAA+AGG | - | Chr1:63680472-63680491 | MsG0180003528.01.T01:CDS | 15.0% |
| !! | ATGCTTGATTATATGAAAAT+TGG | - | Chr1:63680507-63680526 | MsG0180003528.01.T01:CDS | 20.0% |
| !! | TATTTGTGTTTGATGTTTAT+TGG | - | Chr1:63680619-63680638 | MsG0180003528.01.T01:CDS | 20.0% |
| !! | TGCTTGATTATATGAAAATT+GGG | - | Chr1:63680508-63680527 | MsG0180003528.01.T01:CDS | 20.0% |
| ! | ATAAAAACACTCACAATCAA+AGG | + | Chr1:63680594-63680613 | None:intergenic | 25.0% |
| ! | TATGAAAATTGGGTTTGATT+TGG | - | Chr1:63680518-63680537 | MsG0180003528.01.T01:CDS | 25.0% |
| !! | TTTTACAGTAAAAAGGTTCA+TGG | - | Chr1:63680479-63680498 | MsG0180003528.01.T01:CDS | 25.0% |
| AATAAGTAATTAGCTCCAAG+GGG | + | Chr1:63680270-63680289 | None:intergenic | 30.0% | |
| GAATAAGTAATTAGCTCCAA+GGG | + | Chr1:63680271-63680290 | None:intergenic | 30.0% | |
| GTTGTGATTGAACTTACTTT+AGG | - | Chr1:63680674-63680693 | MsG0180003528.01.T01:exon | 30.0% | |
| TGAATAAGTAATTAGCTCCA+AGG | + | Chr1:63680272-63680291 | None:intergenic | 30.0% | |
| ! | AAACCTAAACCCATTGTTTT+CGG | + | Chr1:63680353-63680372 | None:intergenic | 30.0% |
| ! | ATTGGAATTTTCTGAGTGTT+GGG | - | Chr1:63680637-63680656 | MsG0180003528.01.T01:CDS | 30.0% |
| ! | TATTGGAATTTTCTGAGTGT+TGG | - | Chr1:63680636-63680655 | MsG0180003528.01.T01:CDS | 30.0% |
| !! | AGGATTATTGTTTTGTGCTT+GGG | - | Chr1:63680694-63680713 | MsG0180003528.01.T01:five_prime_UTR | 30.0% |
| !! | TAGGATTATTGTTTTGTGCT+TGG | - | Chr1:63680693-63680712 | MsG0180003528.01.T01:five_prime_UTR | 30.0% |
| GACTTTGTTGAAGATTACAG+AGG | - | Chr1:63680551-63680570 | MsG0180003528.01.T01:intron | 35.0% | |
| GGTTTCACTATTGGATTCAT+CGG | - | Chr1:63680368-63680387 | MsG0180003528.01.T01:intron | 35.0% | |
| ! | ATGGGTTTAGGTTTCACTAT+TGG | - | Chr1:63680359-63680378 | MsG0180003528.01.T01:intron | 35.0% |
| ! | ATTACAGAGGAAGAGTTTTC+TGG | - | Chr1:63680564-63680583 | MsG0180003528.01.T01:intron | 35.0% |
| ! | TTACAGAGGAAGAGTTTTCT+GGG | - | Chr1:63680565-63680584 | MsG0180003528.01.T01:intron | 35.0% |
| !!! | CGATCTTTTTTGGTTTCAGA+AGG | + | Chr1:63680328-63680347 | None:intergenic | 35.0% |
| !!! | GTGTTGGGTTTTGATTATGT+AGG | - | Chr1:63680652-63680671 | MsG0180003528.01.T01:CDS | 35.0% |
| !!! | TTTTGGTTTCAGAAGGACAA+AGG | + | Chr1:63680321-63680340 | None:intergenic | 35.0% |
| AAAGATCGCACCGAAAACAA+TGG | - | Chr1:63680340-63680359 | MsG0180003528.01.T01:CDS | 40.0% | |
| AAGATCGCACCGAAAACAAT+GGG | - | Chr1:63680341-63680360 | MsG0180003528.01.T01:intron | 40.0% | |
| ACCTGTTCTCATCAGAATCT+GGG | + | Chr1:63680757-63680776 | None:intergenic | 40.0% | |
| AGGATACACAAAAACTTCCC+TGG | + | Chr1:63680734-63680753 | None:intergenic | 40.0% | |
| TACCTGTTCTCATCAGAATC+TGG | + | Chr1:63680758-63680777 | None:intergenic | 40.0% | |
| !! | GGTTTGATTTGGTTGCAGAT+AGG | - | Chr1:63680529-63680548 | MsG0180003528.01.T01:intron | 40.0% |
| !! | GTTTTCGGTGCGATCTTTTT+TGG | + | Chr1:63680338-63680357 | None:intergenic | 40.0% |
| AAAGGTCGGAGATTTCGATG+AGG | + | Chr1:63680303-63680322 | None:intergenic | 45.0% | |
| GGAGATTTCGATGAGGAACA+CGG | + | Chr1:63680296-63680315 | None:intergenic | 45.0% | |
| GGTTTCAGAAGGACAAAGGT+CGG | + | Chr1:63680317-63680336 | None:intergenic | 45.0% | |
| TCCCAGATTCTGATGAGAAC+AGG | - | Chr1:63680753-63680772 | MsG0180003528.01.T01:five_prime_UTR | 45.0% | |
| TGTTCTCATCAGAATCTGGG+AGG | + | Chr1:63680754-63680773 | None:intergenic | 45.0% | |
| ! | GCACCGAAAACAATGGGTTT+AGG | - | Chr1:63680347-63680366 | MsG0180003528.01.T01:intron | 45.0% |
| ! | GGGCTGCTTTGACTGTGCCA+GGG | - | Chr1:63680714-63680733 | MsG0180003528.01.T01:five_prime_UTR | 60.0% |
| ! | TGGGCTGCTTTGACTGTGCC+AGG | - | Chr1:63680713-63680732 | MsG0180003528.01.T01:five_prime_UTR | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr1 | gene | 63680255 | 63680778 | 63680255 | ID=MsG0180003528.01;Name=MsG0180003528.01 |
| Chr1 | mRNA | 63680255 | 63680778 | 63680255 | ID=MsG0180003528.01.T01;Parent=MsG0180003528.01;Name=MsG0180003528.01.T01;_AED=0.50;_eAED=0.55;_QI=104|0.5|0.33|1|0.5|0.33|3|0|96 |
| Chr1 | exon | 63680255 | 63680359 | 63680255 | ID=MsG0180003528.01.T01:exon:50203;Parent=MsG0180003528.01.T01 |
| Chr1 | exon | 63680434 | 63680546 | 63680434 | ID=MsG0180003528.01.T01:exon:50202;Parent=MsG0180003528.01.T01 |
| Chr1 | exon | 63680602 | 63680778 | 63680602 | ID=MsG0180003528.01.T01:exon:50201;Parent=MsG0180003528.01.T01 |
| Chr1 | five_prime_UTR | 63680675 | 63680778 | 63680675 | ID=MsG0180003528.01.T01:five_prime_utr;Parent=MsG0180003528.01.T01 |
| Chr1 | CDS | 63680602 | 63680674 | 63680602 | ID=MsG0180003528.01.T01:cds;Parent=MsG0180003528.01.T01 |
| Chr1 | CDS | 63680434 | 63680546 | 63680434 | ID=MsG0180003528.01.T01:cds;Parent=MsG0180003528.01.T01 |
| Chr1 | CDS | 63680255 | 63680359 | 63680255 | ID=MsG0180003528.01.T01:cds;Parent=MsG0180003528.01.T01 |
| Gene Sequence |
| Protein sequence |
