AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0180003540.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180003540.01.T02 MTR_1g064060 100.000 341 0 0 1 341 1 341 0.0 710
MsG0180003540.01.T02 MTR_4g461070 96.188 341 13 0 1 341 1 341 0.0 686
MsG0180003540.01.T02 MTR_1g064060 98.997 299 3 0 1 299 1 299 0.0 615
MsG0180003540.01.T02 MTR_3g112040 25.382 327 212 14 12 326 27 333 2.87e-12 67.4
MsG0180003540.01.T02 MTR_3g081400 63.636 44 13 1 263 306 34 74 2.28e-11 59.3
MsG0180003540.01.T01 MTR_1g064060 100.000 341 0 0 1 341 1 341 0.0 710
MsG0180003540.01.T01 MTR_4g461070 96.188 341 13 0 1 341 1 341 0.0 686
MsG0180003540.01.T01 MTR_1g064060 98.997 299 3 0 1 299 1 299 0.0 615
MsG0180003540.01.T01 MTR_3g112040 25.382 327 212 14 12 326 27 333 2.87e-12 67.4
MsG0180003540.01.T01 MTR_3g081400 63.636 44 13 1 263 306 34 74 2.28e-11 59.3
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180003540.01.T02 AT5G03300 83.728 338 55 0 4 341 8 345 0.0 608
MsG0180003540.01.T02 AT3G09820 82.891 339 58 0 3 341 6 344 0.0 598
MsG0180003540.01.T02 AT5G03300 84.247 292 46 0 50 341 1 292 0.0 528
MsG0180003540.01.T02 AT5G03300 84.247 292 46 0 50 341 1 292 0.0 528
MsG0180003540.01.T02 AT3G09820 84.068 295 47 0 47 341 8 302 0.0 526
MsG0180003540.01.T02 AT1G19600 25.904 332 217 14 14 336 23 334 6.93e-13 69.3
MsG0180003540.01.T02 AT4G27600 23.962 313 209 10 7 308 123 417 1.51e-11 65.5
MsG0180003540.01.T01 AT5G03300 83.728 338 55 0 4 341 8 345 0.0 608
MsG0180003540.01.T01 AT3G09820 82.891 339 58 0 3 341 6 344 0.0 598
MsG0180003540.01.T01 AT5G03300 84.247 292 46 0 50 341 1 292 0.0 528
MsG0180003540.01.T01 AT5G03300 84.247 292 46 0 50 341 1 292 0.0 528
MsG0180003540.01.T01 AT3G09820 84.068 295 47 0 47 341 8 302 0.0 526
MsG0180003540.01.T01 AT1G19600 25.904 332 217 14 14 336 23 334 6.93e-13 69.3
MsG0180003540.01.T01 AT4G27600 23.962 313 209 10 7 308 123 417 1.51e-11 65.5

Find 36 sgRNAs with CRISPR-Local

Find 215 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTATTTGAAGTCCTATTATT+TGG 0.128225 1:-63927337 MsG0180003540.01.T01:three_prime_UTR
TTGTCATCTTTAATTTATTT+TGG 0.223522 1:-63927193 MsG0180003540.01.T01:three_prime_UTR
TTTAATAGTTGCACTCTAAC+TGG 0.308482 1:-63927440 MsG0180003540.01.T01:three_prime_UTR
TAATGAAAATCAGGCTTCTC+TGG 0.314330 1:+63927526 None:intergenic
AGTTGCACTCTAACTGGTTT+GGG 0.319640 1:-63927434 MsG0180003540.01.T01:three_prime_UTR
AGATAGGATGCCCAAATAAT+AGG 0.333402 1:+63927326 None:intergenic
TATTTGAAGTCCTATTATTT+GGG 0.348372 1:-63927336 MsG0180003540.01.T01:three_prime_UTR
TGGAGGATTTCTTTCACAAT+TGG 0.357365 1:-63927632 MsG0180003540.01.T01:CDS
TAGTTGCACTCTAACTGGTT+TGG 0.379243 1:-63927435 MsG0180003540.01.T01:three_prime_UTR
GCTCTCACACATTCTTCAAT+AGG 0.380613 1:+63927598 None:intergenic
TTTGTAGGAGATGCCTTTGT+TGG 0.393317 1:-63927652 MsG0180003540.01.T01:intron
AGAATCACTTAATGAAAATC+AGG 0.396079 1:+63927517 None:intergenic
CCCACCCCTTCTTCCTAGCC+GGG 0.396157 1:+63927397 None:intergenic
TAAATGAGAGGTTTATGTTC+TGG 0.397974 1:-63927079 MsG0180003540.01.T01:three_prime_UTR
TTTCATGAGAGTAATCGAAT+TGG 0.401618 1:+63927493 None:intergenic
TCCCACCCCTTCTTCCTAGC+CGG 0.414546 1:+63927396 None:intergenic
GGTAAGTGCAGCCAGACCTT+TGG 0.421838 1:+63927548 None:intergenic
GAGAGGTTTATGTTCTGGTA+TGG 0.427457 1:-63927074 MsG0180003540.01.T01:three_prime_UTR
ACAAACAGCAACAATAAGAT+AGG 0.433012 1:+63927310 None:intergenic
TGTTATTGCACGTTTGTTTG+TGG 0.448590 1:-63927155 MsG0180003540.01.T01:three_prime_UTR
TTCATGAGAGTAATCGAATT+GGG 0.453993 1:+63927494 None:intergenic
GTAGGAGATGCCTTTGTTGG+AGG 0.455368 1:-63927649 MsG0180003540.01.T01:intron
TAAATCCCCGGCTAGGAAGA+AGG 0.467546 1:-63927403 MsG0180003540.01.T01:three_prime_UTR
AATGTTATCATCCAAAGGTC+TGG 0.508644 1:-63927559 MsG0180003540.01.T01:CDS
AGAGGTTTATGTTCTGGTAT+GGG 0.517179 1:-63927073 MsG0180003540.01.T01:three_prime_UTR
AAATCCCCGGCTAGGAAGAA+GGG 0.524488 1:-63927402 MsG0180003540.01.T01:three_prime_UTR
AATGAAAATCAGGCTTCTCT+GGG 0.526760 1:+63927527 None:intergenic
ATTGAAGAATGTGTGAGAGC+TGG 0.541729 1:-63927595 MsG0180003540.01.T01:CDS
TTGAAGAATGTGTGAGAGCT+GGG 0.569874 1:-63927594 MsG0180003540.01.T01:CDS
CCACCCCTTCTTCCTAGCCG+GGG 0.576866 1:+63927398 None:intergenic
CCCCGGCTAGGAAGAAGGGG+TGG 0.577210 1:-63927398 MsG0180003540.01.T01:three_prime_UTR
TGAAAGAAATCCTCCAACAA+AGG 0.578951 1:+63927639 None:intergenic
CAGCAAATGTTATCATCCAA+AGG 0.601761 1:-63927564 MsG0180003540.01.T01:CDS
CCCGGCTAGGAAGAAGGGGT+GGG 0.637180 1:-63927397 MsG0180003540.01.T01:three_prime_UTR
AATCCCCGGCTAGGAAGAAG+GGG 0.654427 1:-63927401 MsG0180003540.01.T01:three_prime_UTR
AAGGGGTGGGAAAGTTGCCG+AGG 0.673131 1:-63927384 MsG0180003540.01.T01:three_prime_UTR

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! CATTTTTTTCAAAATTTTAT+CGG - Chr1:63927909-63927928 MsG0180003540.01.T01:CDS 10.0%
!! TTATTTGAATTTAACAGTTT+AGG + Chr1:63929430-63929449 None:intergenic 15.0%
!!! AATTTTAAAACTGAATTCAA+CGG + Chr1:63928723-63928742 None:intergenic 15.0%
!!! GTAATTTCTTTTATATTAGT+TGG - Chr1:63928476-63928495 MsG0180003540.01.T01:intron 15.0%
!!! TATTTTTATCTCCATTAAAT+GGG - Chr1:63929100-63929119 MsG0180003540.01.T01:intron 15.0%
!!! TTGTCATCTTTAATTTATTT+TGG - Chr1:63930441-63930460 MsG0180003540.01.T01:intron 15.0%
!!! TTGTTGATTTGAATAATAAA+AGG - Chr1:63927316-63927335 MsG0180003540.01.T01:three_prime_UTR 15.0%
!!! TTTATGATTTTTCTTTTTTC+AGG - Chr1:63928409-63928428 MsG0180003540.01.T01:CDS 15.0%
!!! TTTTTAATAAATAGTTGAGA+AGG - Chr1:63929159-63929178 MsG0180003540.01.T01:intron 15.0%
!!! TTTTTTCTTATTGATCTTTA+AGG - Chr1:63929455-63929474 MsG0180003540.01.T01:intron 15.0%
!! ACAATTAGATGCAATAAAAA+AGG + Chr1:63928976-63928995 None:intergenic 20.0%
!! ACTAAATTCATTTAACAACT+AGG - Chr1:63928744-63928763 MsG0180003540.01.T01:intron 20.0%
!! ATCACATCAAATCTTTAATT+AGG + Chr1:63928106-63928125 None:intergenic 20.0%
!! ATTTGATGTGATGTAATAAT+TGG - Chr1:63928114-63928133 MsG0180003540.01.T01:CDS 20.0%
!! TTTATAGATTATTGTGTTTC+CGG - Chr1:63928359-63928378 MsG0180003540.01.T01:intron 20.0%
!!! AAGATAATAAAATTTTGCAC+TGG - Chr1:63928888-63928907 MsG0180003540.01.T01:intron 20.0%
!!! AGATTTTTAGCTGTTTTTAT+CGG - Chr1:63928170-63928189 MsG0180003540.01.T01:intron 20.0%
!!! CTATTTTTATCTCCATTAAA+TGG - Chr1:63929099-63929118 MsG0180003540.01.T01:intron 20.0%
!!! TATTTGAAGTCCTATTATTT+GGG - Chr1:63930298-63930317 MsG0180003540.01.T01:intron 20.0%
!!! TGAGTTTAACTTATTTAGTT+TGG - Chr1:63928049-63928068 MsG0180003540.01.T01:intron 20.0%
!!! TTCTTATTCGAAGTATTAAA+GGG - Chr1:63928863-63928882 MsG0180003540.01.T01:CDS 20.0%
!!! TTCTTTTCTTCTATTTTTGT+AGG - Chr1:63929967-63929986 MsG0180003540.01.T01:intron 20.0%
!!! TTTCTTATTCGAAGTATTAA+AGG - Chr1:63928862-63928881 MsG0180003540.01.T01:CDS 20.0%
!!! TTTGTTGTTGAATAATGTTT+TGG - Chr1:63927519-63927538 MsG0180003540.01.T01:exon 20.0%
!!! TTTTTATTGCATCTAATTGT+AGG - Chr1:63928976-63928995 MsG0180003540.01.T01:intron 20.0%
! AACAGAGATCAAAACAAATA+AGG + Chr1:63927804-63927823 None:intergenic 25.0%
! AATGATAAATTGACCTTGAA+TGG + Chr1:63929924-63929943 None:intergenic 25.0%
! AATTAACTACAAAAAGCAGA+AGG + Chr1:63929849-63929868 None:intergenic 25.0%
! AGAATCACTTAATGAAAATC+AGG + Chr1:63930120-63930139 None:intergenic 25.0%
! ATCAAATACTTCTAGTAGAA+AGG + Chr1:63929301-63929320 None:intergenic 25.0%
! ATGATAAATTGACCTTGAAT+GGG + Chr1:63929923-63929942 None:intergenic 25.0%
! ATTAACTACAAAAAGCAGAA+GGG + Chr1:63929848-63929867 None:intergenic 25.0%
! CACAACTACAAATATAACTT+TGG + Chr1:63929877-63929896 None:intergenic 25.0%
! CTTTAGGTAATAGTATAACA+GGG + Chr1:63929792-63929811 None:intergenic 25.0%
! GAAAAAAAAAATCCCATTCA+AGG - Chr1:63929908-63929927 MsG0180003540.01.T01:intron 25.0%
! GAGACAGAGATTAATATATA+CGG - Chr1:63927667-63927686 MsG0180003540.01.T01:intron 25.0%
! GTATCAACTAATTTCTCTTT+AGG + Chr1:63929808-63929827 None:intergenic 25.0%
! TATACAACAAGATTGAAAGT+AGG - Chr1:63927643-63927662 MsG0180003540.01.T01:CDS 25.0%
! TCAAATACTTCTAGTAGAAA+GGG + Chr1:63929300-63929319 None:intergenic 25.0%
! TCTTTAGGTAATAGTATAAC+AGG + Chr1:63929793-63929812 None:intergenic 25.0%
! TTAACGTTTGTTTGAGTATT+TGG - Chr1:63927711-63927730 MsG0180003540.01.T01:intron 25.0%
! TTATAGATTATTGTGTTTCC+GGG - Chr1:63928360-63928379 MsG0180003540.01.T01:intron 25.0%
! TTCGAATAAGAAAAAATGCA+AGG + Chr1:63928855-63928874 None:intergenic 25.0%
! TTTCATTTAGTAACTCATCT+TGG - Chr1:63929613-63929632 MsG0180003540.01.T01:intron 25.0%
!! AAACCTCTCATTTAGATTTT+TGG + Chr1:63930549-63930568 None:intergenic 25.0%
!! AGATTTTGAATGTGAATGAA+TGG + Chr1:63927137-63927156 None:intergenic 25.0%
!! GTATTTGAAGTCCTATTATT+TGG - Chr1:63930297-63930316 MsG0180003540.01.T01:intron 25.0%
!! TATTTCAATTTTGTCTTCCT+CGG + Chr1:63930270-63930289 None:intergenic 25.0%
!! TCACATGTCTTTTTGTATTT+TGG - Chr1:63930349-63930368 MsG0180003540.01.T01:intron 25.0%
!! TCTCATTTCAGATTCATTTT+CGG - Chr1:63927470-63927489 MsG0180003540.01.T01:three_prime_UTR 25.0%
!! TTAACAGTTTAGGATTCTTA+CGG + Chr1:63929420-63929439 None:intergenic 25.0%
!!! TGGTTTTGAATTTGATTTAC+AGG - Chr1:63927364-63927383 MsG0180003540.01.T01:three_prime_UTR 25.0%
AATGTAAAAGCAAAACTGCA+AGG + Chr1:63929595-63929614 None:intergenic 30.0%
ACAAACAGCAACAATAAGAT+AGG + Chr1:63930327-63930346 None:intergenic 30.0%
ATGTAAAAGCAAAACTGCAA+GGG + Chr1:63929594-63929613 None:intergenic 30.0%
GAGATTAATATATACGGTGT+TGG - Chr1:63927673-63927692 MsG0180003540.01.T01:intron 30.0%
GATGTACTCCACATTATATT+TGG + Chr1:63928298-63928317 None:intergenic 30.0%
GATGTGATGTAATAATTGGT+TGG - Chr1:63928118-63928137 MsG0180003540.01.T01:CDS 30.0%
GCCAGCAATATAGAAATATT+TGG + Chr1:63929185-63929204 None:intergenic 30.0%
TAAAACTGAATTCAACGGTT+CGG + Chr1:63928718-63928737 None:intergenic 30.0%
TAAATGAGAGGTTTATGTTC+TGG - Chr1:63930555-63930574 MsG0180003540.01.T01:five_prime_UTR 30.0%
TCATCAAGATGACTAACATT+TGG - Chr1:63929125-63929144 MsG0180003540.01.T01:intron 30.0%
TCCAAAGCCATTATTATTGA+TGG + Chr1:63927190-63927209 None:intergenic 30.0%
TCCATCAATAATAATGGCTT+TGG - Chr1:63927186-63927205 MsG0180003540.01.T01:three_prime_UTR 30.0%
TTCATGAGAGTAATCGAATT+GGG + Chr1:63930143-63930162 None:intergenic 30.0%
TTCGCATCCATCAATAATAA+TGG - Chr1:63927180-63927199 MsG0180003540.01.T01:three_prime_UTR 30.0%
TTGAATGTGAATGAATGGAA+TGG + Chr1:63927132-63927151 None:intergenic 30.0%
TTTAATAGTTGCACTCTAAC+TGG - Chr1:63930194-63930213 MsG0180003540.01.T01:intron 30.0%
TTTCATGAGAGTAATCGAAT+TGG + Chr1:63930144-63930163 None:intergenic 30.0%
! AACTAATTGAATGGATTCAG+GGG + Chr1:63929224-63929243 None:intergenic 30.0%
! AGAACATTTTCCAAAGTTCA+TGG - Chr1:63929535-63929554 MsG0180003540.01.T01:intron 30.0%
! ATCTTTAAGGATTTGCATTC+TGG - Chr1:63929468-63929487 MsG0180003540.01.T01:intron 30.0%
! CAACTAATTGAATGGATTCA+GGG + Chr1:63929225-63929244 None:intergenic 30.0%
! GAGAAATTAGTTGATACCAA+TGG - Chr1:63929811-63929830 MsG0180003540.01.T01:intron 30.0%
! GCCAAATATTTCTATATTGC+TGG - Chr1:63929181-63929200 MsG0180003540.01.T01:intron 30.0%
! TCTTGATGATTCCCATTTAA+TGG + Chr1:63929114-63929133 None:intergenic 30.0%
! TCTTTAAGGATTTGCATTCT+GGG - Chr1:63929469-63929488 MsG0180003540.01.T01:intron 30.0%
! TGAATCAGATCTCATAGTTT+TGG - Chr1:63927344-63927363 MsG0180003540.01.T01:three_prime_UTR 30.0%
!! AAAAATGTTACTTTCCTTCC+CGG + Chr1:63928381-63928400 None:intergenic 30.0%
!! AGGTATATGGACTATGTTTT+TGG - Chr1:63929496-63929515 MsG0180003540.01.T01:intron 30.0%
!! TCAATAATAATGGCTTTGGA+AGG - Chr1:63927190-63927209 MsG0180003540.01.T01:three_prime_UTR 30.0%
!! TTTGATTTTGCTTCTTACAG+TGG - Chr1:63928539-63928558 MsG0180003540.01.T01:intron 30.0%
!!! CTCTCATTTAGATTTTTGGT+GGG + Chr1:63930545-63930564 None:intergenic 30.0%
!!! TGGGATTTTTTTTAAATCCC+CGG - Chr1:63930219-63930238 MsG0180003540.01.T01:CDS 30.0%
AAGGATTACTGTTATCACAC+AGG - Chr1:63929720-63929739 MsG0180003540.01.T01:intron 35.0%
AATGAAAATCAGGCTTCTCT+GGG + Chr1:63930110-63930129 None:intergenic 35.0%
AATGGCTGCCAAATATAATG+TGG - Chr1:63928287-63928306 MsG0180003540.01.T01:CDS 35.0%
AATGTTATCATCCAAAGGTC+TGG - Chr1:63930075-63930094 MsG0180003540.01.T01:intron 35.0%
AGAGGTTTATGTTCTGGTAT+GGG - Chr1:63930561-63930580 MsG0180003540.01.T01:five_prime_UTR 35.0%
AGATACTGAACAGATCAACA+AGG + Chr1:63929081-63929100 None:intergenic 35.0%
AGATAGGATGCCCAAATAAT+AGG + Chr1:63930311-63930330 None:intergenic 35.0%
AGGATTACTGTTATCACACA+GGG - Chr1:63929721-63929740 MsG0180003540.01.T01:intron 35.0%
ATACTTCTAGTAGAAAGGGA+AGG + Chr1:63929296-63929315 None:intergenic 35.0%
ATGAAATGAGAGAGTGAAGT+AGG + Chr1:63927104-63927123 None:intergenic 35.0%
ATTTATCTGCGAGTTCTTCA+AGG - Chr1:63929374-63929393 MsG0180003540.01.T01:intron 35.0%
CAGCAAATGTTATCATCCAA+AGG - Chr1:63930070-63930089 MsG0180003540.01.T01:intron 35.0%
CCACCAAAAATCTAAATGAG+AGG - Chr1:63930543-63930562 MsG0180003540.01.T01:five_prime_UTR 35.0%
TAATGAAAATCAGGCTTCTC+TGG + Chr1:63930111-63930130 None:intergenic 35.0%
TGAAAGAAATCCTCCAACAA+AGG + Chr1:63929998-63930017 None:intergenic 35.0%
TGCTACTCAGAATTCAATCA+GGG - Chr1:63928432-63928451 MsG0180003540.01.T01:CDS 35.0%
TGGAGGATTTCTTTCACAAT+TGG - Chr1:63930002-63930021 MsG0180003540.01.T01:intron 35.0%
TGTTATTGCACGTTTGTTTG+TGG - Chr1:63930479-63930498 MsG0180003540.01.T01:five_prime_UTR 35.0%
TTCAACGTAATCACAAGTGT+TGG + Chr1:63927885-63927904 None:intergenic 35.0%
TTGAAGAACTCGCAGATAAA+TGG + Chr1:63929375-63929394 None:intergenic 35.0%
TTGTAGTTGTGCTTATGAGT+AGG - Chr1:63929886-63929905 MsG0180003540.01.T01:intron 35.0%
! ACACCTTCGATCAAAAGTAT+TGG + Chr1:63927830-63927849 None:intergenic 35.0%
! ACTAGTTACATTGGTTGCAT+CGG - Chr1:63928583-63928602 MsG0180003540.01.T01:CDS 35.0%
! ATTTGAAGCAACCCGAAAAT+TGG - Chr1:63929045-63929064 MsG0180003540.01.T01:CDS 35.0%
! ATTTTCCAAAGTTCATGGCT+GGG - Chr1:63929540-63929559 MsG0180003540.01.T01:intron 35.0%
! CTTGTGCTAGAATGCTATTT+AGG - Chr1:63927757-63927776 MsG0180003540.01.T01:intron 35.0%
! GCAACTAATTGAATGGATTC+AGG + Chr1:63929226-63929245 None:intergenic 35.0%
! TGTTTGAGTCTTTGAGAGTT+TGG - Chr1:63928073-63928092 MsG0180003540.01.T01:intron 35.0%
! TTTGAAGCAACCCGAAAATT+GGG - Chr1:63929046-63929065 MsG0180003540.01.T01:CDS 35.0%
! TTTTCCATCTCTCAGTTTTC+TGG - Chr1:63928145-63928164 MsG0180003540.01.T01:intron 35.0%
!! CCTCTCATTTAGATTTTTGG+TGG + Chr1:63930546-63930565 None:intergenic 35.0%
!! TCTAACCTTGTTATTTGCTG+CGG + Chr1:63929257-63929276 None:intergenic 35.0%
!!! AATTTTCGGGTTGCTTCAAA+TGG + Chr1:63929046-63929065 None:intergenic 35.0%
!!! CATTTAGATTTTTGGTGGGT+AGG + Chr1:63930541-63930560 None:intergenic 35.0%
!!! TTTTTTTTAAATCCCCGGCT+AGG - Chr1:63930224-63930243 MsG0180003540.01.T01:CDS 35.0%
AAAGATATCCCAGTTGCCAA+AGG - Chr1:63929681-63929700 MsG0180003540.01.T01:intron 40.0%
AAATGAGAGAGTGAAGTAGG+TGG + Chr1:63927101-63927120 None:intergenic 40.0%
ACCAAGAACTCTAAACAAGC+TGG - Chr1:63928631-63928650 MsG0180003540.01.T01:CDS 40.0%
AGATTATTGTGTTTCCGGGA+AGG - Chr1:63928364-63928383 MsG0180003540.01.T01:intron 40.0%
AGTATCTTACTCCCTTGTGA+AGG - Chr1:63929326-63929345 MsG0180003540.01.T01:intron 40.0%
ATCAGCTGCAAATTGCTACA+AGG - Chr1:63929016-63929035 MsG0180003540.01.T01:CDS 40.0%
ATGCTCGGCAACTAATTGAA+TGG + Chr1:63929233-63929252 None:intergenic 40.0%
ATTGAAGAATGTGTGAGAGC+TGG - Chr1:63930039-63930058 MsG0180003540.01.T01:intron 40.0%
CAGACAACCAAAATGAAGCA+CGG + Chr1:63927967-63927986 None:intergenic 40.0%
CATCATGAAGACCTTCACAA+GGG + Chr1:63929340-63929359 None:intergenic 40.0%
CTTTCCAGAAAACTGAGAGA+TGG + Chr1:63928152-63928171 None:intergenic 40.0%
GAAGATGAGAATACACCTAC+AGG - Chr1:63928778-63928797 MsG0180003540.01.T01:intron 40.0%
GAGAGGTTTATGTTCTGGTA+TGG - Chr1:63930560-63930579 MsG0180003540.01.T01:five_prime_UTR 40.0%
GATGCAACCAATGTAACTAG+TGG + Chr1:63928584-63928603 None:intergenic 40.0%
GCAATTTGCAGCTGATAAGT+TGG + Chr1:63929012-63929031 None:intergenic 40.0%
GCTCTCACACATTCTTCAAT+AGG + Chr1:63930039-63930058 None:intergenic 40.0%
TAGTTGCACTCTAACTGGTT+TGG - Chr1:63930199-63930218 MsG0180003540.01.T01:intron 40.0%
TATAATGTGGAGTACATCGC+TGG - Chr1:63928300-63928319 MsG0180003540.01.T01:intron 40.0%
TATGATCCTACGACTGTTTC+AGG - Chr1:63928256-63928275 MsG0180003540.01.T01:CDS 40.0%
TCATCATGAAGACCTTCACA+AGG + Chr1:63929341-63929360 None:intergenic 40.0%
TGCATCGGAAAAGACAAGTT+TGG - Chr1:63928598-63928617 MsG0180003540.01.T01:CDS 40.0%
TTGAAGAATGTGTGAGAGCT+GGG - Chr1:63930040-63930059 MsG0180003540.01.T01:intron 40.0%
TTTGTAGGAGATGCCTTTGT+TGG - Chr1:63929982-63930001 MsG0180003540.01.T01:intron 40.0%
! ACATTTTCAGTCTGGTCAAC+AGG + Chr1:63929646-63929665 None:intergenic 40.0%
! ACCAGCTTGTTTAGAGTTCT+TGG + Chr1:63928635-63928654 None:intergenic 40.0%
! AGTTGCACTCTAACTGGTTT+GGG - Chr1:63930200-63930219 MsG0180003540.01.T01:intron 40.0%
! CATTTTCCAAAGTTCATGGC+TGG - Chr1:63929539-63929558 MsG0180003540.01.T01:intron 40.0%
! CTGTTTCAGGTACGATGAAA+TGG - Chr1:63928269-63928288 MsG0180003540.01.T01:CDS 40.0%
! GCACCAATACTTTTGATCGA+AGG - Chr1:63927824-63927843 MsG0180003540.01.T01:CDS 40.0%
! GGTACCTAATGCCCAATTTT+CGG + Chr1:63929060-63929079 None:intergenic 40.0%
! GTACCTAATGCCCAATTTTC+GGG + Chr1:63929059-63929078 None:intergenic 40.0%
! GTGCTACTCAGAATTCAATC+AGG - Chr1:63928431-63928450 MsG0180003540.01.T01:CDS 40.0%
! GTGTTTGACACGAAAAGTGT+AGG + Chr1:63927863-63927882 None:intergenic 40.0%
! TTAGTTGATACCAATGGAGC+AGG - Chr1:63929817-63929836 MsG0180003540.01.T01:intron 40.0%
!! CATTCTGGGATTCAGGTATA+TGG - Chr1:63929483-63929502 MsG0180003540.01.T01:intron 40.0%
ATCGTACCTGAAACAGTCGT+AGG + Chr1:63928265-63928284 None:intergenic 45.0%
CAAACAGCACAAGTTCCTGT+AGG + Chr1:63928796-63928815 None:intergenic 45.0%
CAACCCGAAAATTGGGCATT+AGG - Chr1:63929053-63929072 MsG0180003540.01.T01:CDS 45.0%
CCATCTTGAGCAACACATAC+GGG + Chr1:63929754-63929773 None:intergenic 45.0%
CCCATCTTGAGCAACACATA+CGG + Chr1:63929755-63929774 None:intergenic 45.0%
CGAGAATGGCATTGTTAAGC+TGG + Chr1:63927398-63927417 None:intergenic 45.0%
CGCAGATAAATGGTGCAGAA+AGG + Chr1:63929365-63929384 None:intergenic 45.0%
GAATTCAATCAGGGTTGCTC+AGG - Chr1:63928441-63928460 MsG0180003540.01.T01:CDS 45.0%
GGATGCGAAAAAACACAGAG+AGG + Chr1:63927169-63927188 None:intergenic 45.0%
GTGCTTATCTTCAGCGAGAA+TGG + Chr1:63927412-63927431 None:intergenic 45.0%
TGACCAGACTGAAAATGTCG+AGG - Chr1:63929648-63929667 MsG0180003540.01.T01:intron 45.0%
TGGATTCAGGGGATACAGTA+AGG + Chr1:63929213-63929232 None:intergenic 45.0%
! CACGAAAAGTGTAGGACATC+AGG + Chr1:63927855-63927874 None:intergenic 45.0%
! CCGTATGTGTTGCTCAAGAT+GGG - Chr1:63929752-63929771 MsG0180003540.01.T01:intron 45.0%
! CGGAAAAGACAAGTTTGGTG+AGG - Chr1:63928603-63928622 MsG0180003540.01.T01:CDS 45.0%
! TCTCCTCGACATTTTCAGTC+TGG + Chr1:63929654-63929673 None:intergenic 45.0%
! TCTGTGTCCGTGCTTCATTT+TGG - Chr1:63927957-63927976 MsG0180003540.01.T01:CDS 45.0%
!! ACTTGTGCTGTTTGTGTGGT+TGG - Chr1:63928802-63928821 MsG0180003540.01.T01:intron 45.0%
!! AGGAACTTGTGCTGTTTGTG+TGG - Chr1:63928798-63928817 MsG0180003540.01.T01:intron 45.0%
!! GAAGGTGTTCTTCTCGGTAT+GGG - Chr1:63927208-63927227 MsG0180003540.01.T01:intron 45.0%
!! GCTTTGGAAGGTGTTCTTCT+CGG - Chr1:63927202-63927221 MsG0180003540.01.T01:intron 45.0%
!! GGATTTGCATTCTGGGATTC+AGG - Chr1:63929476-63929495 MsG0180003540.01.T01:intron 45.0%
!! TTTGTGTGGTTGGTGGTGAA+AGG - Chr1:63928812-63928831 MsG0180003540.01.T01:intron 45.0%
AAATCCCCGGCTAGGAAGAA+GGG - Chr1:63930232-63930251 MsG0180003540.01.T01:CDS 50.0%
AAGGGCAATACCTGCTCCAT+TGG + Chr1:63929830-63929849 None:intergenic 50.0%
AATGTGGAGTACATCGCTGG+AGG - Chr1:63928303-63928322 MsG0180003540.01.T01:intron 50.0%
ACAGCAGAGATGTCGAGAAG+GGG + Chr1:63927238-63927257 None:intergenic 50.0%
ACCAATGTAACTAGTGGCCC+CGG + Chr1:63928578-63928597 None:intergenic 50.0%
CGAGTTCTTCAAGGATCCAC+AGG - Chr1:63929383-63929402 MsG0180003540.01.T01:intron 50.0%
CTCTGATGCCTTTGGCAACT+GGG + Chr1:63929692-63929711 None:intergenic 50.0%
GACAGCAGAGATGTCGAGAA+GGG + Chr1:63927239-63927258 None:intergenic 50.0%
GCATGCCGCAGCAAATAACA+AGG - Chr1:63929249-63929268 MsG0180003540.01.T01:intron 50.0%
GTAGGAGATGCCTTTGTTGG+AGG - Chr1:63929985-63930004 MsG0180003540.01.T01:intron 50.0%
TAAATCCCCGGCTAGGAAGA+AGG - Chr1:63930231-63930250 MsG0180003540.01.T01:CDS 50.0%
TACCTCCCAGCCATGAACTT+TGG + Chr1:63929548-63929567 None:intergenic 50.0%
TTCCAAAGTTCATGGCTGGG+AGG - Chr1:63929543-63929562 MsG0180003540.01.T01:intron 50.0%
! AGTGGATGCTTCAAGTTCCC+GGG - Chr1:63928557-63928576 MsG0180003540.01.T01:intron 50.0%
! CAGTGGATGCTTCAAGTTCC+CGG - Chr1:63928556-63928575 MsG0180003540.01.T01:intron 50.0%
! CCCGTATGTGTTGCTCAAGA+TGG - Chr1:63929751-63929770 MsG0180003540.01.T01:intron 50.0%
! GTTATTTGCTGCGGCATGCT+CGG + Chr1:63929248-63929267 None:intergenic 50.0%
!! AACGGTTCGGTACAGTGTAG+TGG + Chr1:63928705-63928724 None:intergenic 50.0%
!! GGAAGGTGTTCTTCTCGGTA+TGG - Chr1:63927207-63927226 MsG0180003540.01.T01:intron 50.0%
!! GGTTGGTGGTGAAAGGTAAG+AGG - Chr1:63928819-63928838 MsG0180003540.01.T01:intron 50.0%
!! TGTGCTGTTTGTGTGGTTGG+TGG - Chr1:63928805-63928824 MsG0180003540.01.T01:intron 50.0%
AATCCCCGGCTAGGAAGAAG+GGG - Chr1:63930233-63930252 MsG0180003540.01.T01:CDS 55.0%
CCAATGTAACTAGTGGCCCC+GGG + Chr1:63928577-63928596 None:intergenic 55.0%
CCTCTGATGCCTTTGGCAAC+TGG + Chr1:63929693-63929712 None:intergenic 55.0%
CGACAGCAGAGATGTCGAGA+AGG + Chr1:63927240-63927259 None:intergenic 55.0%
GGTAAGTGCAGCCAGACCTT+TGG + Chr1:63930089-63930108 None:intergenic 55.0%
TACGGCAGAGCTTTCTCCTG+TGG + Chr1:63929402-63929421 None:intergenic 55.0%
TTGCGTGCCTCTGATGCCTT+TGG + Chr1:63929700-63929719 None:intergenic 55.0%
! AGGCATCAGAGGCACGCAAA+AGG - Chr1:63929701-63929720 MsG0180003540.01.T01:intron 55.0%
! GTGGATGCTTCAAGTTCCCG+GGG - Chr1:63928558-63928577 MsG0180003540.01.T01:intron 55.0%
!! CCAGTTGCCAAAGGCATCAG+AGG - Chr1:63929690-63929709 MsG0180003540.01.T01:intron 55.0%
AAGGGGTGGGAAAGTTGCCG+AGG - Chr1:63930250-63930269 MsG0180003540.01.T01:CDS 60.0%
CCCGGGGCCACTAGTTACAT+TGG - Chr1:63928574-63928593 MsG0180003540.01.T01:intron 60.0%
TCCCACCCCTTCTTCCTAGC+CGG + Chr1:63930241-63930260 None:intergenic 60.0%
CCACCCCTTCTTCCTAGCCG+GGG + Chr1:63930239-63930258 None:intergenic 65.0%
CCCACCCCTTCTTCCTAGCC+GGG + Chr1:63930240-63930259 None:intergenic 65.0%
CCCGGCTAGGAAGAAGGGGT+GGG - Chr1:63930237-63930256 MsG0180003540.01.T01:CDS 65.0%
CCCCGGCTAGGAAGAAGGGG+TGG - Chr1:63930236-63930255 MsG0180003540.01.T01:CDS 70.0%
Chromosome Type Strat End Strand Name
Chr1 gene 63927046 63930610 63927046 ID=MsG0180003540.01;Name=MsG0180003540.01
Chr1 mRNA 63927046 63930610 63927046 ID=MsG0180003540.01.T01;Parent=MsG0180003540.01;Name=MsG0180003540.01.T01;_AED=0.48;_eAED=0.48;_QI=153|1|1|1|0.91|0.84|13|457|341
Chr1 exon 63927046 63927216 63927046 ID=MsG0180003540.01.T01:exon:4768;Parent=MsG0180003540.01.T01
Chr1 exon 63927239 63927667 63927239 ID=MsG0180003540.01.T01:exon:4767;Parent=MsG0180003540.01.T01
Chr1 five_prime_UTR 63930458 63930610 63930458 ID=MsG0180003540.01.T01:five_prime_utr;Parent=MsG0180003540.01.T01
Chr1 CDS 63930375 63930457 63930375 ID=MsG0180003540.01.T01:cds;Parent=MsG0180003540.01.T01
Chr1 CDS 63930217 63930270 63930217 ID=MsG0180003540.01.T01:cds;Parent=MsG0180003540.01.T01
Chr1 CDS 63929332 63929378 63929332 ID=MsG0180003540.01.T01:cds;Parent=MsG0180003540.01.T01
Chr1 CDS 63929194 63929225 63929194 ID=MsG0180003540.01.T01:cds;Parent=MsG0180003540.01.T01
Chr1 CDS 63928996 63929097 63928996 ID=MsG0180003540.01.T01:cds;Parent=MsG0180003540.01.T01
Chr1 CDS 63928823 63928890 63928823 ID=MsG0180003540.01.T01:cds;Parent=MsG0180003540.01.T01
Chr1 CDS 63928582 63928658 63928582 ID=MsG0180003540.01.T01:cds;Parent=MsG0180003540.01.T01
Chr1 CDS 63928386 63928483 63928386 ID=MsG0180003540.01.T01:cds;Parent=MsG0180003540.01.T01
Chr1 CDS 63928238 63928308 63928238 ID=MsG0180003540.01.T01:cds;Parent=MsG0180003540.01.T01
Chr1 CDS 63928092 63928158 63928092 ID=MsG0180003540.01.T01:cds;Parent=MsG0180003540.01.T01
Chr1 CDS 63927818 63928001 63927818 ID=MsG0180003540.01.T01:cds;Parent=MsG0180003540.01.T01
Chr1 CDS 63927525 63927667 63927525 ID=MsG0180003540.01.T01:cds;Parent=MsG0180003540.01.T01
Chr1 three_prime_UTR 63927239 63927524 63927239 ID=MsG0180003540.01.T01:three_prime_utr;Parent=MsG0180003540.01.T01
Chr1 three_prime_UTR 63927046 63927216 63927046 ID=MsG0180003540.01.T01:three_prime_utr;Parent=MsG0180003540.01.T01
Chr1 mRNA 63927183 63930610 63927183 ID=MsG0180003540.01.T02;Parent=MsG0180003540.01;Name=MsG0180003540.01.T02;_AED=0.48;_eAED=0.48;_QI=153|1|1|1|1|1|12|342|341
Chr1 exon 63927183 63927667 63927183 ID=MsG0180003540.01.T02:exon:4769;Parent=MsG0180003540.01.T02
Chr1 five_prime_UTR 63930458 63930610 63930458 ID=MsG0180003540.01.T02:five_prime_utr;Parent=MsG0180003540.01.T02
Chr1 CDS 63930375 63930457 63930375 ID=MsG0180003540.01.T02:cds;Parent=MsG0180003540.01.T02
Chr1 CDS 63930217 63930270 63930217 ID=MsG0180003540.01.T02:cds;Parent=MsG0180003540.01.T02
Chr1 CDS 63929332 63929378 63929332 ID=MsG0180003540.01.T02:cds;Parent=MsG0180003540.01.T02
Chr1 CDS 63929194 63929225 63929194 ID=MsG0180003540.01.T02:cds;Parent=MsG0180003540.01.T02
Chr1 CDS 63928996 63929097 63928996 ID=MsG0180003540.01.T02:cds;Parent=MsG0180003540.01.T02
Chr1 CDS 63928823 63928890 63928823 ID=MsG0180003540.01.T02:cds;Parent=MsG0180003540.01.T02
Chr1 CDS 63928582 63928658 63928582 ID=MsG0180003540.01.T02:cds;Parent=MsG0180003540.01.T02
Chr1 CDS 63928386 63928483 63928386 ID=MsG0180003540.01.T02:cds;Parent=MsG0180003540.01.T02
Chr1 CDS 63928238 63928308 63928238 ID=MsG0180003540.01.T02:cds;Parent=MsG0180003540.01.T02
Chr1 CDS 63928092 63928158 63928092 ID=MsG0180003540.01.T02:cds;Parent=MsG0180003540.01.T02
Chr1 CDS 63927818 63928001 63927818 ID=MsG0180003540.01.T02:cds;Parent=MsG0180003540.01.T02
Chr1 CDS 63927525 63927667 63927525 ID=MsG0180003540.01.T02:cds;Parent=MsG0180003540.01.T02
Chr1 three_prime_UTR 63927183 63927524 63927183 ID=MsG0180003540.01.T02:three_prime_utr;Parent=MsG0180003540.01.T02
Gene Sequence

>MsG0180003540.01.T02

ATGGCTTTGGAAGGTGTTCTTCTCGGTATGGGTAATCCCCTTCTCGACATCTCTGCTGTCGTTGATGAAGATTTCTTGAAAAAGTTTGATATCCAGCTTAACAATGCCATTCTCGCTGAAGATAAGCACAAATCTATGTACGATGAAATGGCTGCCAAATATAATGTGGAGTACATCGCTGGAGGTGCTACTCAGAATTCAATCAGGGTTGCTCAGTGGATGCTTCAAGTTCCCGGGGCCACTAGTTACATTGGTTGCATCGGAAAAGACAAGTTTGGTGAGGAGATGACCAAGAACTCTAAACAAGCTGGTGTAAATGTTCACTATTATGAAGATGAGAATACACCTACAGGAACTTGTGCTGTTTGTGTGGTTGGTGGTGAAAGGTCACTTATTGCCAACTTATCAGCTGCAAATTGCTACAAGGTCGACCATTTGAAGCAACCCGAAAATTGGGCATTAGTTGAGAAGGCCAAATATTTCTATATTGCTGGCTTTTTCCTTACTGTATCCCCTGAATCCATTCAATTAGTTGCCGAGCATGCCGCAGCAAATAACAAGGTCTTCATGATGAACCTTTCTGCACCATTTATCTGCGAGTTCTTCAAGGATCCACAGGAGAAAGCTCTGCCGTATATGGACTATGTTTTTGGAAATGAGACTGAAGCAAGAACATTTTCCAAAGTTCATGGCTGGGAGACTGAAAATGTCGAGGAGATAGCTTTAAAGATATCCCAGTTGCCAAAGGCATCAGAGGCACGCAAAAGGATTACTGTTATCACACAGGGTGCTGATCCCGTATGTGTTGCTCAAGATGGGAAAGTGACATTGTACCCTGTTATACTATTACCTAAAGAGAAATTAGTTGATACCAATGGAGCAGGAGATGCCTTTGTTGGAGGATTTCTTTCACAATTGGTTCAAGAGAAGCCTATTGAAGAATGTGTGAGAGCTGGGTGTTATGCAGCAAATGTTATCATCCAAAGGTCTGGCTGCACTTACCCAGAGAAGCCTGATTTTCATTAA

>MsG0180003540.01.T01

ATGGCTTTGGAAGGTGTTCTTCTCGGTATGGGTAATCCCCTTCTCGACATCTCTGCTGTCGTTGATGAAGATTTCTTGAAAAAGTTTGATATCCAGCTTAACAATGCCATTCTCGCTGAAGATAAGCACAAATCTATGTACGATGAAATGGCTGCCAAATATAATGTGGAGTACATCGCTGGAGGTGCTACTCAGAATTCAATCAGGGTTGCTCAGTGGATGCTTCAAGTTCCCGGGGCCACTAGTTACATTGGTTGCATCGGAAAAGACAAGTTTGGTGAGGAGATGACCAAGAACTCTAAACAAGCTGGTGTAAATGTTCACTATTATGAAGATGAGAATACACCTACAGGAACTTGTGCTGTTTGTGTGGTTGGTGGTGAAAGGTCACTTATTGCCAACTTATCAGCTGCAAATTGCTACAAGGTCGACCATTTGAAGCAACCCGAAAATTGGGCATTAGTTGAGAAGGCCAAATATTTCTATATTGCTGGCTTTTTCCTTACTGTATCCCCTGAATCCATTCAATTAGTTGCCGAGCATGCCGCAGCAAATAACAAGGTCTTCATGATGAACCTTTCTGCACCATTTATCTGCGAGTTCTTCAAGGATCCACAGGAGAAAGCTCTGCCGTATATGGACTATGTTTTTGGAAATGAGACTGAAGCAAGAACATTTTCCAAAGTTCATGGCTGGGAGACTGAAAATGTCGAGGAGATAGCTTTAAAGATATCCCAGTTGCCAAAGGCATCAGAGGCACGCAAAAGGATTACTGTTATCACACAGGGTGCTGATCCCGTATGTGTTGCTCAAGATGGGAAAGTGACATTGTACCCTGTTATACTATTACCTAAAGAGAAATTAGTTGATACCAATGGAGCAGGAGATGCCTTTGTTGGAGGATTTCTTTCACAATTGGTTCAAGAGAAGCCTATTGAAGAATGTGTGAGAGCTGGGTGTTATGCAGCAAATGTTATCATCCAAAGGTCTGGCTGCACTTACCCAGAGAAGCCTGATTTTCATTAA
Protein sequence

>MsG0180003540.01.T02

MALEGVLLGMGNPLLDISAVVDEDFLKKFDIQLNNAILAEDKHKSMYDEMAAKYNVEYIAGGATQNSIRVAQWMLQVPGATSYIGCIGKDKFGEEMTKNSKQAGVNVHYYEDENTPTGTCAVCVVGGERSLIANLSAANCYKVDHLKQPENWALVEKAKYFYIAGFFLTVSPESIQLVAEHAAANNKVFMMNLSAPFICEFFKDPQEKALPYMDYVFGNETEARTFSKVHGWETENVEEIALKISQLPKASEARKRITVITQGADPVCVAQDGKVTLYPVILLPKEKLVDTNGAGDAFVGGFLSQLVQEKPIEECVRAGCYAANVIIQRSGCTYPEKPDFH*

>MsG0180003540.01.T01

MALEGVLLGMGNPLLDISAVVDEDFLKKFDIQLNNAILAEDKHKSMYDEMAAKYNVEYIAGGATQNSIRVAQWMLQVPGATSYIGCIGKDKFGEEMTKNSKQAGVNVHYYEDENTPTGTCAVCVVGGERSLIANLSAANCYKVDHLKQPENWALVEKAKYFYIAGFFLTVSPESIQLVAEHAAANNKVFMMNLSAPFICEFFKDPQEKALPYMDYVFGNETEARTFSKVHGWETENVEEIALKISQLPKASEARKRITVITQGADPVCVAQDGKVTLYPVILLPKEKLVDTNGAGDAFVGGFLSQLVQEKPIEECVRAGCYAANVIIQRSGCTYPEKPDFH*