AlfalfaGEDB | MsG0480021150.01

M. sativa cultivar ZhongmuNo.1 / MsG0480021150.01

KEGG: K00847
GO: GO:0016301 GO:0016773
PFAM: PF00294

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480021150.01.T01	XP_013456308.1	95.627	343	3	1	1	343	1	331	0	653

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480021150.01.T01	Q42896	83.133	332	44	1	10	341	6	325	0	559

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080048773.01	MsG0480021150.01	-0.810648	1.038124e-50	6.292994e-48
MsG0080049040.01	MsG0480021150.01	-0.820066	8.344901e-53	6.535791e-50
MsG0080049068.01	MsG0480021150.01	-0.810838	9.444062e-51	5.754152e-48
MsG0180001270.01	MsG0480021150.01	-0.801460	8.911204e-49	4.258017e-46
MsG0180001880.01	MsG0480021150.01	-0.802222	6.213832e-49	3.027098e-46
MsG0180003353.01	MsG0480021150.01	-0.817710	2.864010e-52	2.101330e-49
MsG0180003821.01	MsG0480021150.01	-0.852528	4.352510e-61	9.120421e-58
MsG0180005418.01	MsG0480021150.01	-0.812498	4.112711e-51	2.619149e-48
MsG0180005807.01	MsG0480021150.01	-0.826360	2.831245e-54	2.649379e-51
MsG0180006104.01	MsG0480021150.01	-0.814939	1.193158e-51	8.114623e-49
MsG0280010564.01	MsG0480021150.01	-0.815074	1.113713e-51	7.602128e-49
MsG0280010926.01	MsG0480021150.01	-0.815664	8.233226e-52	5.711769e-49
MsG0280011026.01	MsG0480021150.01	-0.822594	2.178882e-53	1.832073e-50
MsG0280011073.01	MsG0480021150.01	-0.818755	1.660763e-52	1.254086e-49
MsG0380012860.01	MsG0480021150.01	-0.802444	5.594532e-49	2.740910e-46
MsG0380014584.01	MsG0480021150.01	-0.822925	1.824426e-53	1.548350e-50
MsG0480018848.01	MsG0480021150.01	-0.817973	2.498053e-52	1.845985e-49
MsG0480018911.01	MsG0480021150.01	-0.805771	1.136555e-49	6.064924e-47
MsG0480019007.01	MsG0480021150.01	-0.843154	1.668727e-58	2.587214e-55
MsG0480020408.01	MsG0480021150.01	-0.825253	5.185330e-54	4.701571e-51
MsG0480020690.01	MsG0480021150.01	-0.826812	2.209467e-54	2.094345e-51
MsG0480020783.01	MsG0480021150.01	-0.807273	5.478698e-50	3.038567e-47
MsG0480021150.01	MsG0480021739.01	-0.800295	1.540811e-48	7.148768e-46
MsG0480021150.01	MsG0480022166.01	-0.832283	1.032567e-55	1.148688e-52
MsG0480021150.01	MsG0480022239.01	-0.811261	7.648722e-51	4.712480e-48
MsG0480021150.01	MsG0480022401.01	-0.862845	3.796785e-64	1.127828e-60
MsG0480021150.01	MsG0480022537.01	-0.811268	7.622690e-51	4.697224e-48
MsG0480021150.01	MsG0480022921.01	-0.824492	7.838142e-54	6.953399e-51
MsG0480021150.01	MsG0480023367.01	-0.808731	2.681911e-50	1.545132e-47
MsG0480021150.01	MsG0480023867.01	-0.810597	1.064771e-50	6.445673e-48
MsG0480021150.01	MsG0580024679.01	-0.801711	7.913822e-49	3.805479e-46
MsG0480021150.01	MsG0580024873.01	-0.836450	9.291064e-57	1.171342e-53
MsG0480021150.01	MsG0580028024.01	-0.825362	4.885549e-54	4.443323e-51
MsG0480021150.01	MsG0580028111.01	-0.802915	4.472184e-49	2.217514e-46
MsG0480021150.01	MsG0580029529.01	-0.820645	6.146936e-53	4.891372e-50
MsG0480021150.01	MsG0580029912.01	-0.810465	1.137240e-50	6.860028e-48
MsG0480021150.01	MsG0680031115.01	-0.863627	2.174636e-64	6.636280e-61
MsG0480021150.01	MsG0680031469.01	-0.842981	1.854682e-58	2.860068e-55
MsG0480021150.01	MsG0680033377.01	-0.804063	2.585844e-49	1.320559e-46
MsG0480021150.01	MsG0680034064.01	-0.848271	6.818529e-60	1.244533e-56
MsG0480021150.01	MsG0680035307.01	-0.843724	1.175067e-58	1.855108e-55
MsG0480021150.01	MsG0780036263.01	-0.818835	1.592715e-52	1.205398e-49
MsG0480021150.01	MsG0780037399.01	-0.834192	3.454887e-56	4.069372e-53
MsG0480021150.01	MsG0780038784.01	-0.803266	3.783492e-49	1.892895e-46
MsG0480021150.01	MsG0780039975.01	-0.809688	1.671841e-50	9.881629e-48
MsG0480021150.01	MsG0780040284.01	-0.850751	1.387050e-60	2.744937e-57
MsG0480021150.01	MsG0780040825.01	0.806122	9.589914e-50	5.163431e-47
MsG0480021150.01	MsG0780041091.01	-0.835748	1.400779e-56	1.728968e-53
MsG0480021150.01	MsG0780041482.01	-0.815369	9.578815e-52	6.591621e-49
MsG0480021150.01	MsG0780041729.01	0.817872	2.631603e-52	1.939437e-49
MsG0480021150.01	MsG0880042695.01	-0.809449	1.881720e-50	1.105119e-47
MsG0480021150.01	MsG0880044392.01	-0.812789	3.552751e-51	2.279713e-48
MsG0480021150.01	MsG0880044589.01	0.820498	6.643524e-53	5.265396e-50
MsG0480021150.01	MsG0880044884.01	-0.816597	5.095187e-52	3.626056e-49
MsG0480021150.01	MsG0880045044.01	-0.801117	1.047642e-48	4.962927e-46
MsG0480021150.01	MsG0880046723.01	-0.814325	1.631462e-51	1.091369e-48
MsG0480021150.01	MsG0880046765.01	-0.801517	8.673914e-49	4.150670e-46
MsG0480021150.01	MsG0880046867.01	-0.800030	1.744912e-48	8.040990e-46
MsG0480021150.01	MsG0880047422.01	0.811187	7.935492e-51	4.879673e-48
MsG0480021150.01	MsG0880047656.01	-0.842188	3.011095e-58	4.529348e-55
MsG0480021150.01	MsG0880047703.01	-0.802066	6.690565e-49	3.246495e-46
MsG0380015603.01	MsG0480021150.01	-0.821546	3.812372e-53	3.111022e-50
MsG0380016194.01	MsG0480021150.01	-0.848042	7.883390e-60	1.428195e-56
MsG0380016284.01	MsG0480021150.01	-0.835609	1.518607e-56	1.866510e-53
MsG0380017087.01	MsG0480021150.01	-0.816308	5.912705e-52	4.175040e-49
MsG0380017728.01	MsG0480021150.01	-0.854593	1.109973e-61	2.491828e-58
MsG0280007062.01	MsG0480021150.01	-0.801066	1.072786e-48	5.075619e-46
MsG0280008929.01	MsG0480021150.01	-0.809094	2.242371e-50	1.304538e-47
MsG0280009685.01	MsG0480021150.01	-0.848653	5.342586e-60	9.873019e-57

PPI

Gene1	Gene2	Type
MsG0580028982.01	MsG0480021150.01	PPI
MsG0180004312.01	MsG0480021150.01	PPI
MsG0480021150.01	MsG0480020848.01	PPI
MsG0280009908.01	MsG0480021150.01	PPI
MsG0680033932.01	MsG0480021150.01	PPI
MsG0580026320.01	MsG0480021150.01	PPI
MsG0180003540.01	MsG0480021150.01	PPI
MsG0480021150.01	MsG0480023996.01	PPI
MsG0180003540.01	MsG0480021150.01	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480021150.01.T01	MTR_4g067310	95.627	343	3	1	1	343	1	331	0.0	653
MsG0480021150.01.T01	MTR_2g098950	85.924	341	35	2	1	341	1	328	0.0	586
MsG0480021150.01.T01	MTR_1g076850	65.031	326	102	1	15	340	8	321	1.64e-153	434
MsG0480021150.01.T01	MTR_1g101950	62.952	332	109	2	13	342	21	340	1.90e-145	414
MsG0480021150.01.T01	MTR_5g079460	64.653	331	104	2	13	342	65	383	1.38e-144	414
MsG0480021150.01.T01	MTR_6g089480	60.494	324	114	2	22	343	1	312	5.53e-140	399
MsG0480021150.01.T01	MTR_6g089480	61.348	282	95	2	22	301	1	270	8.10e-122	352
MsG0480021150.01.T01	MTR_6g089480	60.915	284	97	2	22	303	1	272	9.02e-122	352
MsG0480021150.01.T01	MTR_7g075860	55.152	330	132	4	15	340	41	358	2.30e-120	351
MsG0480021150.01.T01	MTR_1g069745	50.327	306	126	4	36	339	4	285	1.25e-97	291
MsG0480021150.01.T01	MTR_1g057810	48.780	328	146	5	14	338	252	560	1.88e-95	294
MsG0480021150.01.T01	MTR_1g069490	48.193	332	132	5	13	342	108	401	2.24e-93	284
MsG0480021150.01.T01	MTR_1g105150	45.732	328	142	6	14	338	252	546	2.77e-83	263
MsG0480021150.01.T01	MTR_1g054730	43.662	284	119	2	55	338	144	386	2.81e-67	216
MsG0480021150.01.T01	MTR_4g019165	30.667	375	209	5	8	339	86	452	1.77e-47	167
MsG0480021150.01.T01	MTR_5g044990	32.484	314	183	6	41	336	250	552	1.41e-46	166
MsG0480021150.01.T01	MTR_0046s0180	49.558	113	45	1	226	338	8	108	1.71e-29	109
MsG0480021150.01.T01	MTR_2g072830	27.961	304	175	10	46	340	89	357	6.68e-17	81.3

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0480021150.01.T01	AT2G31390	80.409	342	49	2	1	342	1	324	0.0	552
MsG0480021150.01.T01	AT1G06030	81.402	328	49	1	14	341	10	325	0.0	551
MsG0480021150.01.T01	AT3G59480	82.263	327	46	1	15	341	10	324	0.0	550
MsG0480021150.01.T01	AT1G06020	80.183	328	53	1	14	341	9	324	0.0	540
MsG0480021150.01.T01	AT4G10260	66.972	327	96	1	15	341	6	320	1.68e-154	437
MsG0480021150.01.T01	AT1G66430	62.952	332	110	2	13	343	63	382	1.12e-149	427
MsG0480021150.01.T01	AT5G51830	63.720	328	105	2	15	340	23	338	1.66e-145	414
MsG0480021150.01.T01	AT5G51830	63.720	328	105	2	15	340	23	338	1.66e-145	414
MsG0480021150.01.T01	AT1G50390	60.656	183	31	2	160	342	1	142	8.40e-69	212
MsG0480021150.01.T01	AT3G54090	30.952	378	209	6	8	342	96	464	7.64e-49	171
MsG0480021150.01.T01	AT1G69200	30.068	296	181	4	41	319	246	532	3.65e-38	144
MsG0480021150.01.T01	AT1G17160	29.126	309	169	14	46	341	105	376	4.22e-17	82.0

Find 120 sgRNAs with CRISPR-Local

Find 257 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
ACAAGAAGTAACTTCAAATT+AGG	0.127503	4:-54393964	None:intergenic
GAACTACTGGTTACAAAATT+TGG	0.157679	4:-54395321	None:intergenic
GGTAACATCATATATGATTT+TGG	0.182404	4:+54395277	MsG0480021150.01.T01:three_prime_UTR
CAGGCAAAAGTCTTCCATTA+TGG	0.241889	4:+54393688	MsG0480021150.01.T01:intron
CTTGTAACTCTTGGTGAAAA+TGG	0.245978	4:+54393982	MsG0480021150.01.T01:CDS
GGATTGAATTACGCGATTAA+TGG	0.257475	4:-54391792	None:intergenic
GTAAGCAAATTCTCAGTATA+TGG	0.266378	4:+54393845	MsG0480021150.01.T01:CDS
CTAAGCACTCACTTCAAGTA+TGG	0.282419	4:-54394863	None:intergenic
AGCAATTACTACTACCAAAA+AGG	0.287374	4:+54395043	MsG0480021150.01.T01:CDS
AGCGTTGGAGGCCATTGGTT+TGG	0.288462	4:-54391953	None:intergenic
CGGAGCCGGTGGCGGGGAGG+AGG	0.303525	4:-54391978	None:intergenic
AGTTCAAGATTGAGATCTTC+AGG	0.313198	4:-54392345	None:intergenic
GATTGAATTACGCGATTAAT+GGG	0.319921	4:-54391791	None:intergenic
AAGAGTGATTATAAAGGATT+AGG	0.321324	4:-54391813	None:intergenic
GCGTTGGAGGCCATTGGTTT+GGG	0.333327	4:-54391952	None:intergenic
CGTAGTGAGAAGGAAGAATA+TGG	0.334379	4:-54391891	None:intergenic
GGGGAGGAGGGAAGCGTTGG+AGG	0.336425	4:-54391965	None:intergenic
ATTTGGAAGAAAACATTGTT+TGG	0.348265	4:-54395304	None:intergenic
AACAGGATGAAGCAAGGTTA+AGG	0.370203	4:+54394991	MsG0480021150.01.T01:intron
TCAAATCTTTATTGAACTAC+TGG	0.374237	4:-54395334	None:intergenic
TCCTTCGTTGGAGCTCTGTT+GGG	0.375710	4:+54394820	MsG0480021150.01.T01:CDS
TGTCCTTAGCCTGATCAAAA+AGG	0.382490	4:+54395097	MsG0480021150.01.T01:CDS
CTCCTCCCCGCCACCGGCTC+CGG	0.383160	4:+54391979	MsG0480021150.01.T01:CDS
CAAGAAGTAACTTCAAATTA+GGG	0.389192	4:-54393963	None:intergenic
TTCCTTCGTTGGAGCTCTGT+TGG	0.395933	4:+54394819	MsG0480021150.01.T01:CDS
TCAAACTTATCGATCCATAA+TGG	0.398109	4:-54393702	None:intergenic
CAAACCTCCGGCTACCTTTG+TGG	0.410022	4:+54393803	MsG0480021150.01.T01:CDS
TATACTGAGAATTTGCTTAC+GGG	0.413418	4:-54393842	None:intergenic
TAGGAAAAGAGTGATTATAA+AGG	0.418420	4:-54391819	None:intergenic
CACAAAGGTAGCCGGAGGTT+TGG	0.427528	4:-54393802	None:intergenic
GTCTCCCTTGCTGAAGCTCC+CGG	0.431068	4:+54392057	MsG0480021150.01.T01:CDS
GGGAGACACCAGAGGTTGTT+GGG	0.432776	4:-54392041	None:intergenic
TAGGTGGAGTTTATTTATAT+AGG	0.433041	4:-54391838	None:intergenic
TCAGCTTCTGTTGGAAGAGC+TGG	0.436019	4:-54395074	None:intergenic
AAGTTACTTCTTGTAACTCT+TGG	0.438912	4:+54393973	MsG0480021150.01.T01:CDS
ATATACTGAGAATTTGCTTA+CGG	0.441354	4:-54393843	None:intergenic
GACTTCGTCCCAACAACCTC+TGG	0.441931	4:+54392033	MsG0480021150.01.T01:CDS
AATTCTGATTTGTCCAGAAA+AGG	0.443374	4:-54394738	None:intergenic
GACGTTAGCTGGTGCACCAC+CGG	0.450576	4:-54392094	None:intergenic
ACAGGATGAAGCAAGGTTAA+GGG	0.455931	4:+54394992	MsG0480021150.01.T01:intron
GTGGAGCTGGAATTTCTAAC+TGG	0.457777	4:+54393898	MsG0480021150.01.T01:CDS
ACAGCGATCGCGACGTTAGC+TGG	0.464087	4:-54392105	None:intergenic
ATGAGACCGGAGCCGGTGGC+GGG	0.469238	4:-54391985	None:intergenic
TCCGGTCTCATCGTCAGCTT+CGG	0.470421	4:+54391997	MsG0480021150.01.T01:CDS
ATTTGCTTACGGGCTTCCTC+TGG	0.470871	4:-54393832	None:intergenic
GGTGCTGGTGATTCCTTCGT+TGG	0.473983	4:+54394808	MsG0480021150.01.T01:CDS
AATTCGGTCACATGCTCGCC+GGG	0.475756	4:+54392178	MsG0480021150.01.T01:CDS
TAAGCAAATTCTCAGTATAT+GGG	0.478304	4:+54393846	MsG0480021150.01.T01:CDS
GTTCTTCTATCAATTTAAGT+TGG	0.480048	4:+54395201	MsG0480021150.01.T01:three_prime_UTR
GTCAACACTGTTGATACAAC+TGG	0.481252	4:+54394787	MsG0480021150.01.T01:CDS
CAAGGTCAGTGATGTGGAGC+TGG	0.482074	4:+54393885	MsG0480021150.01.T01:CDS
GGCGGGGAGGAGGGAAGCGT+TGG	0.487671	4:-54391968	None:intergenic
TCTGCCTTCGTCGGAAAACT+CGG	0.489892	4:+54392147	MsG0480021150.01.T01:CDS
AGGGAAGCGTTGGAGGCCAT+TGG	0.492348	4:-54391958	None:intergenic
TGGACAAATCAGAATTTCAA+AGG	0.493890	4:+54394745	MsG0480021150.01.T01:CDS
AGGGAGACACCAGAGGTTGT+TGG	0.499418	4:-54392042	None:intergenic
GGAGCCGGTGGCGGGGAGGA+GGG	0.504246	4:-54391977	None:intergenic
CTTAAATTTGCCAATGCATG+TGG	0.505670	4:+54395020	MsG0480021150.01.T01:CDS
ACGTTAGCTGGTGCACCACC+GGG	0.506368	4:-54392093	None:intergenic
CTAAGGACATCAGCTTCTGT+TGG	0.510882	4:-54395083	None:intergenic
GAAGGAAGAATATGGATATG+AGG	0.514039	4:-54391883	None:intergenic
GCTTCCCTCCTCCCCGCCAC+CGG	0.518084	4:+54391973	MsG0480021150.01.T01:CDS
AAACTCGGTGATGATGAATT+CGG	0.518336	4:+54392162	MsG0480021150.01.T01:CDS
ACTTCAAATTAGGGTGCCAC+AGG	0.521764	4:-54393954	None:intergenic
TACAAGAGAGTGTGATTAGG+TGG	0.524345	4:-54391854	None:intergenic
TGGATCGATAAGTTTGATTG+TGG	0.524813	4:+54393708	MsG0480021150.01.T01:CDS
GAAAACAACGTGAGAGGTGA+TGG	0.526062	4:+54392210	MsG0480021150.01.T01:CDS
CCCGGTTTCCTCAAAGCCCC+CGG	0.527234	4:+54392075	MsG0480021150.01.T01:CDS
ATGGAAGTTGCTAAGGAGGC+AGG	0.531001	4:+54393760	MsG0480021150.01.T01:CDS
TGAGACCGGAGCCGGTGGCG+GGG	0.532555	4:-54391984	None:intergenic
GAATTCGGTCACATGCTCGC+CGG	0.537803	4:+54392177	MsG0480021150.01.T01:CDS
TTACGGGCTTCCTCTGGTGA+AGG	0.541696	4:-54393826	None:intergenic
ACTGTTGATACAACTGGTGC+TGG	0.543600	4:+54394793	MsG0480021150.01.T01:CDS
AGTAATTGCTCCACATGCAT+TGG	0.546129	4:-54395030	None:intergenic
CAAAGGCGCTCGTACTGCAC+TGG	0.546743	4:+54392245	MsG0480021150.01.T01:CDS
GAAGGCAATGGAAGTTGCTA+AGG	0.548461	4:+54393753	MsG0480021150.01.T01:CDS
GCCGAAGCTGACGATGAGAC+CGG	0.549381	4:-54391998	None:intergenic
GATGAGACCGGAGCCGGTGG+CGG	0.549666	4:-54391986	None:intergenic
ATATACAAGAGAGTGTGATT+AGG	0.550330	4:-54391857	None:intergenic
GTGAAGGCCACAAAGGTAGC+CGG	0.553387	4:-54393810	None:intergenic
TTCAGATGTGCTGATCTGCA+TGG	0.553886	4:-54393733	None:intergenic
TTAAATAACAGGATGAAGCA+AGG	0.555208	4:+54394985	MsG0480021150.01.T01:intron
ACCTTTGTGGCCTTCACCAG+AGG	0.557240	4:+54393816	MsG0480021150.01.T01:CDS
GATTTGAGTCCACATTGTCT+CGG	0.557259	4:+54395355	MsG0480021150.01.T01:three_prime_UTR
GCGATCGCTGTTTCACGACT+CGG	0.559904	4:+54392117	MsG0480021150.01.T01:CDS
GGCAATGGAAGTTGCTAAGG+AGG	0.567696	4:+54393756	MsG0480021150.01.T01:CDS
TCTCTTATGATCCAAACCTC+CGG	0.571114	4:+54393791	MsG0480021150.01.T01:CDS
ATTTCCTTTCTGCATATCAA+CGG	0.572308	4:+54395256	MsG0480021150.01.T01:three_prime_UTR
ATGCAGATCAGCACATCTGA+AGG	0.574430	4:+54393735	MsG0480021150.01.T01:CDS
GCTGACGATGAGACCGGAGC+CGG	0.576648	4:-54391992	None:intergenic
GATGGTATCAACTTTGACAA+AGG	0.579902	4:+54392228	MsG0480021150.01.T01:CDS
TTGTATCAACAGTGTTGACA+TGG	0.579990	4:-54394783	None:intergenic
ATTTGAATAACGTAGTGAGA+AGG	0.580855	4:-54391901	None:intergenic
GACCGGAGCCGGTGGCGGGG+AGG	0.584412	4:-54391981	None:intergenic
TCCTCTGGTGAAGGCCACAA+AGG	0.588528	4:-54393817	None:intergenic
GACGATGAGACCGGAGCCGG+TGG	0.591172	4:-54391989	None:intergenic
ATCAGCACATCTGAAGGCAA+TGG	0.591539	4:+54393741	MsG0480021150.01.T01:CDS
GCCCAACAGAGCTCCAACGA+AGG	0.601582	4:-54394821	None:intergenic
TTGAAAGAAAACAACGTGAG+AGG	0.604412	4:+54392204	MsG0480021150.01.T01:CDS
TCTCATCAAGGTCAGTGATG+TGG	0.606659	4:+54393879	MsG0480021150.01.T01:CDS
GTTACCGTTGATATGCAGAA+AGG	0.607874	4:-54395260	None:intergenic
CATGAACTCACGCTCTCCAT+CGG	0.608461	4:-54392286	None:intergenic
TTTGTTACTCTACGCGCCGA+TGG	0.610292	4:+54392270	MsG0480021150.01.T01:CDS
GGCGGTAAATCTGCCTTCGT+CGG	0.612156	4:+54392138	MsG0480021150.01.T01:CDS
TTCAGCAAGGGAGACACCAG+AGG	0.627638	4:-54392049	None:intergenic
CTCACAACATCCCAAACCAA+TGG	0.632258	4:+54391942	MsG0480021150.01.T01:exon
ATCAGTTGGCCGAGACAATG+TGG	0.632522	4:-54395364	None:intergenic
GGACAAGGCTGATCTCATCA+AGG	0.638589	4:+54393867	MsG0480021150.01.T01:CDS
GGAAACCGGGAGCTTCAGCA+AGG	0.638648	4:-54392062	None:intergenic
ATGCTTCTGCTCTCTCCCTG+TGG	0.639152	4:+54393938	MsG0480021150.01.T01:CDS
GAAACCGGGAGCTTCAGCAA+GGG	0.654816	4:-54392061	None:intergenic
GTTAGCTGGTGCACCACCGG+GGG	0.660853	4:-54392091	None:intergenic
GTAAGAAGCATATCAGCACT+CGG	0.662701	4:-54392321	None:intergenic
AATTCTCAGTATATGGGACA+AGG	0.663851	4:+54393852	MsG0480021150.01.T01:CDS
ATCGCTGTTTCACGACTCGG+CGG	0.669298	4:+54392120	MsG0480021150.01.T01:CDS
CGTTAGCTGGTGCACCACCG+GGG	0.671336	4:-54392092	None:intergenic
CTTCAAATTAGGGTGCCACA+GGG	0.672272	4:-54393953	None:intergenic
AATTGATAGAAGAACTCCAG+CGG	0.685971	4:-54395193	None:intergenic
AAGGCCACAAAGGTAGCCGG+AGG	0.689884	4:-54393807	None:intergenic
TGGTTCCAGATATTATACAA+AGG	0.703810	4:+54394002	MsG0480021150.01.T01:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	TTATATTTTACGTTATATAT+TGG	-	Chr4:54392552-54392571	None:intergenic	10.0%
!!	AATAAGTAAAATTGAAGATT+AGG	-	Chr4:54394035-54394054	None:intergenic	15.0%
!!	AGAATAAAAAAAAAAAGGTT+AGG	-	Chr4:54393320-54393339	None:intergenic	15.0%
!!	ATATACTATTATGACTTAAT+TGG	+	Chr4:54393027-54393046	MsG0480021150.01.T01:intron	15.0%
!!!	AGTTGATTTTATTAGTTTTA+GGG	+	Chr4:54393079-54393098	MsG0480021150.01.T01:intron	15.0%
!!!	TAGTTGATTTTATTAGTTTT+AGG	+	Chr4:54393078-54393097	MsG0480021150.01.T01:intron	15.0%
!!!	TATTTTTCGTTTTAAATAAC+AGG	+	Chr4:54394974-54394993	MsG0480021150.01.T01:intron	15.0%
!!!	TTAATTTCTTTGTTTTTCAA+AGG	-	Chr4:54393203-54393222	None:intergenic	15.0%
!!	TATCCATACTTGTATTATAT+TGG	+	Chr4:54394903-54394922	MsG0480021150.01.T01:intron	20.0%
!!	TTTATTTATACGATCAGAAA+AGG	+	Chr4:54392605-54392624	MsG0480021150.01.T01:intron	20.0%
!!!	ATTATGAAACATGATTTTTC+AGG	+	Chr4:54393669-54393688	MsG0480021150.01.T01:intron	20.0%
!!!	TCATAATTCAATTACTCTTT+TGG	-	Chr4:54393656-54393675	None:intergenic	20.0%
!!!	TTTTACCTTTGTATAATATC+TGG	-	Chr4:54394010-54394029	None:intergenic	20.0%
!!!	TTTTTGATCTGATTTTTTTG+TGG	+	Chr4:54392422-54392441	MsG0480021150.01.T01:intron	20.0%
!	AACTATAGTGAATCAAAAAC+AGG	-	Chr4:54392928-54392947	None:intergenic	25.0%
!	AAGAGTGATTATAAAGGATT+AGG	-	Chr4:54391816-54391835	None:intergenic	25.0%
!	ACAAGAAGTAACTTCAAATT+AGG	-	Chr4:54393967-54393986	None:intergenic	25.0%
!	ACACCAATATAATACAAGTA+TGG	-	Chr4:54394909-54394928	None:intergenic	25.0%
!	ATCAGAAAAGGTGATATATA+TGG	+	Chr4:54392617-54392636	MsG0480021150.01.T01:intron	25.0%
!	ATTTGGAAGAAAACATTGTT+TGG	-	Chr4:54395307-54395326	None:intergenic	25.0%
!	CAAGAAGTAACTTCAAATTA+GGG	-	Chr4:54393966-54393985	None:intergenic	25.0%
!	GGTTCAAATTCAATTGAAAA+AGG	+	Chr4:54394334-54394353	MsG0480021150.01.T01:intron	25.0%
!	GTTCTTCTATCAATTTAAGT+TGG	+	Chr4:54395201-54395220	MsG0480021150.01.T01:three_prime_UTR	25.0%
!	TAAGCAAATTCTCAGTATAT+GGG	+	Chr4:54393846-54393865	MsG0480021150.01.T01:CDS	25.0%
!	TAGGAAAAGAGTGATTATAA+AGG	-	Chr4:54391822-54391841	None:intergenic	25.0%
!	TAGGTGGAGTTTATTTATAT+AGG	-	Chr4:54391841-54391860	None:intergenic	25.0%
!	TCAAATCTTTATTGAACTAC+TGG	-	Chr4:54395337-54395356	None:intergenic	25.0%
!	TCAGAAAAGGTGATATATAT+GGG	+	Chr4:54392618-54392637	MsG0480021150.01.T01:intron	25.0%
!	TCCATTCAATAACTTAACTA+GGG	+	Chr4:54394449-54394468	MsG0480021150.01.T01:intron	25.0%
!	TCTAACGAATCAAAATACAA+AGG	-	Chr4:54392887-54392906	None:intergenic	25.0%
!	TTAAGAATTCGAATACCAAA+CGG	+	Chr4:54393458-54393477	MsG0480021150.01.T01:intron	25.0%
!	TTCCATTCAATAACTTAACT+AGG	+	Chr4:54394448-54394467	MsG0480021150.01.T01:intron	25.0%
!	TTGTTTACAGTTGAAAAGTA+AGG	-	Chr4:54394398-54394417	None:intergenic	25.0%
!!	AACATGCAATATTGTTATTG+AGG	-	Chr4:54394663-54394682	None:intergenic	25.0%
!!	AATCAAAATCTGCATTGTTT+TGG	+	Chr4:54393557-54393576	MsG0480021150.01.T01:intron	25.0%
!!	AGTTTATAGAGTGTTAATTG+TGG	-	Chr4:54393178-54393197	None:intergenic	25.0%
!!	ATATACTGAGAATTTGCTTA+CGG	-	Chr4:54393846-54393865	None:intergenic	25.0%
!!	GGTAAATGTGTGTATAATTT+TGG	+	Chr4:54393048-54393067	MsG0480021150.01.T01:intron	25.0%
!!	GGTAACATCATATATGATTT+TGG	+	Chr4:54395277-54395296	MsG0480021150.01.T01:three_prime_UTR	25.0%
!!!	GTTTTAGAAAGTATGTTAAG+TGG	+	Chr4:54394680-54394699	MsG0480021150.01.T01:intron	25.0%
!!!	TAAGTTGGTTTTTATCTCTT+TGG	+	Chr4:54395216-54395235	MsG0480021150.01.T01:three_prime_UTR	25.0%
!!!	TTTTCTAAAATATATGCACG+TGG	-	Chr4:54393236-54393255	None:intergenic	25.0%
	AAGTTACTTCTTGTAACTCT+TGG	+	Chr4:54393973-54393992	MsG0480021150.01.T01:CDS	30.0%
	AGAAAAGGTGATATATATGG+GGG	+	Chr4:54392620-54392639	MsG0480021150.01.T01:intron	30.0%
	AGCAATTACTACTACCAAAA+AGG	+	Chr4:54395043-54395062	MsG0480021150.01.T01:CDS	30.0%
	ATGTGTGCAAGTAGTAAAAT+TGG	+	Chr4:54393401-54393420	MsG0480021150.01.T01:intron	30.0%
	ATTTCCTTTCTGCATATCAA+CGG	+	Chr4:54395256-54395275	MsG0480021150.01.T01:three_prime_UTR	30.0%
	ATTTGAATAACGTAGTGAGA+AGG	-	Chr4:54391904-54391923	None:intergenic	30.0%
	CAGAAAAGGTGATATATATG+GGG	+	Chr4:54392619-54392638	MsG0480021150.01.T01:intron	30.0%
	CATTCAATAACTTAACTAGG+GGG	+	Chr4:54394451-54394470	MsG0480021150.01.T01:intron	30.0%
	CCATTCAATAACTTAACTAG+GGG	+	Chr4:54394450-54394469	MsG0480021150.01.T01:intron	30.0%
	GATTGAATTACGCGATTAAT+GGG	-	Chr4:54391794-54391813	None:intergenic	30.0%
	GCAATTACTACTACCAAAAA+GGG	+	Chr4:54395044-54395063	MsG0480021150.01.T01:CDS	30.0%
	GGTAGAAAATTGATCAAGAT+TGG	-	Chr4:54393588-54393607	None:intergenic	30.0%
	GTAAGCAAATTCTCAGTATA+TGG	+	Chr4:54393845-54393864	MsG0480021150.01.T01:CDS	30.0%
	TATCACACTATGTTAGAAGT+GGG	-	Chr4:54392741-54392760	None:intergenic	30.0%
	TATCCAAACAAGATCATCAT+TGG	-	Chr4:54394139-54394158	None:intergenic	30.0%
	TCAAACTTATCGATCCATAA+TGG	-	Chr4:54393705-54393724	None:intergenic	30.0%
	TGGACAAATCAGAATTTCAA+AGG	+	Chr4:54394745-54394764	MsG0480021150.01.T01:CDS	30.0%
	TGGTTCCAGATATTATACAA+AGG	+	Chr4:54394002-54394021	MsG0480021150.01.T01:CDS	30.0%
	TTAAAAACCGAGCTTGAATT+TGG	-	Chr4:54393526-54393545	None:intergenic	30.0%
	TTAAATAACAGGATGAAGCA+AGG	+	Chr4:54394985-54395004	MsG0480021150.01.T01:intron	30.0%
	TTACAGTTGAAAAGTAAGGT+GGG	-	Chr4:54394394-54394413	None:intergenic	30.0%
	TTATCGTGACGTTTATTGAT+TGG	+	Chr4:54392816-54392835	MsG0480021150.01.T01:intron	30.0%
	TTGAATTTGAACCTAACTAC+TGG	-	Chr4:54394327-54394346	None:intergenic	30.0%
	TTGGATCATCTGATCTTAAT+TGG	-	Chr4:54393507-54393526	None:intergenic	30.0%
	TTGTAAGTCTGTTTATGACA+TGG	-	Chr4:54394295-54394314	None:intergenic	30.0%
	TTTACAGTTGAAAAGTAAGG+TGG	-	Chr4:54394395-54394414	None:intergenic	30.0%
	TTTCCAATGATGATCTTGTT+TGG	+	Chr4:54394133-54394152	MsG0480021150.01.T01:intron	30.0%
!	AATTCTGATTTGTCCAGAAA+AGG	-	Chr4:54394741-54394760	None:intergenic	30.0%
!	ATATACAAGAGAGTGTGATT+AGG	-	Chr4:54391860-54391879	None:intergenic	30.0%
!	ATTTCTATGCCTTTTTGATC+AGG	-	Chr4:54395109-54395128	None:intergenic	30.0%
!	GAACTACTGGTTACAAAATT+TGG	-	Chr4:54395324-54395343	None:intergenic	30.0%
!	TATACTGAGAATTTGCTTAC+GGG	-	Chr4:54393845-54393864	None:intergenic	30.0%
!	TCTTTTGGCATGAAAAAAAC+TGG	-	Chr4:54393641-54393660	None:intergenic	30.0%
!	TGACAATTTTTCTCCTTTTC+TGG	+	Chr4:54394725-54394744	MsG0480021150.01.T01:CDS	30.0%
	AAATGCTTATGCCAGTAGTT+AGG	+	Chr4:54394313-54394332	MsG0480021150.01.T01:intron	35.0%
	AATATATGCACGTGGTTTCT+AGG	-	Chr4:54393228-54393247	None:intergenic	35.0%
	AATTCTCAGTATATGGGACA+AGG	+	Chr4:54393852-54393871	MsG0480021150.01.T01:CDS	35.0%
	AATTGATAGAAGAACTCCAG+CGG	-	Chr4:54395196-54395215	None:intergenic	35.0%
	ACTACAATCTAAATGCAGAG+AGG	-	Chr4:54394615-54394634	None:intergenic	35.0%
	AGATGATCCAAATTCAAGCT+CGG	+	Chr4:54393516-54393535	MsG0480021150.01.T01:intron	35.0%
	AGTACTACAATACCCTATGT+AGG	-	Chr4:54394105-54394124	None:intergenic	35.0%
	AGTTCAAGATTGAGATCTTC+AGG	-	Chr4:54392348-54392367	None:intergenic	35.0%
	CCCCTAGTTAAGTTATTGAA+TGG	-	Chr4:54394453-54394472	None:intergenic	35.0%
	CTACAATCTAAATGCAGAGA+GGG	-	Chr4:54394614-54394633	None:intergenic	35.0%
	CTTAAATTTGCCAATGCATG+TGG	+	Chr4:54395020-54395039	MsG0480021150.01.T01:CDS	35.0%
	CTTGTAACTCTTGGTGAAAA+TGG	+	Chr4:54393982-54394001	MsG0480021150.01.T01:CDS	35.0%
	GAAAAGGTGATATATATGGG+GGG	+	Chr4:54392621-54392640	MsG0480021150.01.T01:intron	35.0%
	GAAGGAAGAATATGGATATG+AGG	-	Chr4:54391886-54391905	None:intergenic	35.0%
	GATGGTATCAACTTTGACAA+AGG	+	Chr4:54392228-54392247	MsG0480021150.01.T01:CDS	35.0%
	GGATTGAATTACGCGATTAA+TGG	-	Chr4:54391795-54391814	None:intergenic	35.0%
	GTATCACACTATGTTAGAAG+TGG	-	Chr4:54392742-54392761	None:intergenic	35.0%
	TAACATAGTGTGATACACTG+TGG	+	Chr4:54392746-54392765	MsG0480021150.01.T01:intron	35.0%
	TGGATCGATAAGTTTGATTG+TGG	+	Chr4:54393708-54393727	MsG0480021150.01.T01:CDS	35.0%
	TTATGGTTAATCCCACACAT+TGG	+	Chr4:54393341-54393360	MsG0480021150.01.T01:intron	35.0%
	TTGAAAGAAAACAACGTGAG+AGG	+	Chr4:54392204-54392223	MsG0480021150.01.T01:CDS	35.0%
	TTTCACTCTTAGCCTACATA+GGG	+	Chr4:54394090-54394109	MsG0480021150.01.T01:intron	35.0%
!	AAACTCGGTGATGATGAATT+CGG	+	Chr4:54392162-54392181	MsG0480021150.01.T01:CDS	35.0%
!	GCAAGTAGTAAAATTGGTCT+AGG	+	Chr4:54393407-54393426	MsG0480021150.01.T01:intron	35.0%
!	TAAAATTGGTCTAGGTCACT+AGG	+	Chr4:54393415-54393434	MsG0480021150.01.T01:intron	35.0%
!!	CATAGGGTATTGTAGTACTT+TGG	+	Chr4:54394106-54394125	MsG0480021150.01.T01:intron	35.0%
!!	GTTGTTTTCTTTCAAGATCC+CGG	-	Chr4:54392199-54392218	None:intergenic	35.0%
!!	TTGTATCAACAGTGTTGACA+TGG	-	Chr4:54394786-54394805	None:intergenic	35.0%
!!!	TACGCTTTTTTTGGTGAAGT+AGG	+	Chr4:54392701-54392720	MsG0480021150.01.T01:intron	35.0%
	AAACCGCGCATGATATAGTA+GGG	-	Chr4:54393272-54393291	None:intergenic	40.0%
	AACAGGATGAAGCAAGGTTA+AGG	+	Chr4:54394991-54395010	MsG0480021150.01.T01:intron	40.0%
	AACTCCAGCGGAAAAATGTA+AGG	-	Chr4:54395184-54395203	None:intergenic	40.0%
	AATAACTTAACTAGGGGGAG+GGG	+	Chr4:54394456-54394475	MsG0480021150.01.T01:intron	40.0%
	ACAGGATGAAGCAAGGTTAA+GGG	+	Chr4:54394992-54395011	MsG0480021150.01.T01:intron	40.0%
	ACTCCAGCGGAAAAATGTAA+GGG	-	Chr4:54395183-54395202	None:intergenic	40.0%
	AGTAATTGCTCCACATGCAT+TGG	-	Chr4:54395033-54395052	None:intergenic	40.0%
	ATACATTCACACCTGTGCTA+TGG	+	Chr4:54394532-54394551	MsG0480021150.01.T01:intron	40.0%
	CAATAACTTAACTAGGGGGA+GGG	+	Chr4:54394455-54394474	MsG0480021150.01.T01:intron	40.0%
	CAGGCAAAAGTCTTCCATTA+TGG	+	Chr4:54393688-54393707	MsG0480021150.01.T01:intron	40.0%
	CCAGCAAGCAATAGAGAAAA+AGG	-	Chr4:54394058-54394077	None:intergenic	40.0%
	CGTAGTGAGAAGGAAGAATA+TGG	-	Chr4:54391894-54391913	None:intergenic	40.0%
	CTAAGCACTCACTTCAAGTA+TGG	-	Chr4:54394866-54394885	None:intergenic	40.0%
	CTTGCACACATTTGATGTGT+CGG	-	Chr4:54393392-54393411	None:intergenic	40.0%
	CTTTCACTCTTAGCCTACAT+AGG	+	Chr4:54394089-54394108	MsG0480021150.01.T01:intron	40.0%
	GAATTCGAATACCAAACGGT+TGG	+	Chr4:54393462-54393481	MsG0480021150.01.T01:intron	40.0%
	GATGAGAGAGTGATGGTTTA+AGG	+	Chr4:54393109-54393128	MsG0480021150.01.T01:intron	40.0%
	GATTTGAGTCCACATTGTCT+CGG	+	Chr4:54395355-54395374	MsG0480021150.01.T01:three_prime_UTR	40.0%
	GCATTATCGCATGCAAATGA+AGG	+	Chr4:54393151-54393170	MsG0480021150.01.T01:intron	40.0%
	GTCAACACTGTTGATACAAC+TGG	+	Chr4:54394787-54394806	MsG0480021150.01.T01:CDS	40.0%
	GTTACCGTTGATATGCAGAA+AGG	-	Chr4:54395263-54395282	None:intergenic	40.0%
	TAAACCGCGCATGATATAGT+AGG	-	Chr4:54393273-54393292	None:intergenic	40.0%
	TCAATAACTTAACTAGGGGG+AGG	+	Chr4:54394454-54394473	MsG0480021150.01.T01:intron	40.0%
	TCAATTCGATGAGAGAGTGA+TGG	+	Chr4:54393102-54393121	MsG0480021150.01.T01:intron	40.0%
	TCTCTTATGATCCAAACCTC+CGG	+	Chr4:54393791-54393810	MsG0480021150.01.T01:CDS	40.0%
	TGTCCTTAGCCTGATCAAAA+AGG	+	Chr4:54395097-54395116	MsG0480021150.01.T01:CDS	40.0%
	TTGCACACATTTGATGTGTC+GGG	-	Chr4:54393391-54393410	None:intergenic	40.0%
!	AGTCAGATTTTCCATGTGGT+GGG	+	Chr4:54392504-54392523	MsG0480021150.01.T01:intron	40.0%
!	ATGCCTTTTTGATCAGGCTA+AGG	-	Chr4:54395103-54395122	None:intergenic	40.0%
!	CCTTTTTCTCTATTGCTTGC+TGG	+	Chr4:54394055-54394074	MsG0480021150.01.T01:intron	40.0%
!	CTTAATTGGATCAAAAGCGC+TGG	-	Chr4:54393493-54393512	None:intergenic	40.0%
!	GAGGGGCTTACTATATTTTG+TGG	+	Chr4:54394473-54394492	MsG0480021150.01.T01:intron	40.0%
!	GTAAGAAGCATATCAGCACT+CGG	-	Chr4:54392324-54392343	None:intergenic	40.0%
!	GTACTGTTGTGTTGCTATCA+TGG	+	Chr4:54392846-54392865	MsG0480021150.01.T01:intron	40.0%
!	GTGACGTTTATTGATTGGAG+TGG	+	Chr4:54392821-54392840	MsG0480021150.01.T01:intron	40.0%
!	TACAAGAGAGTGTGATTAGG+TGG	-	Chr4:54391857-54391876	None:intergenic	40.0%
!!	TTTTTGGTGAAGTAGGCAAG+TGG	+	Chr4:54392708-54392727	MsG0480021150.01.T01:intron	40.0%
!!!	TGGCTAGCTTACGCTTTTTT+TGG	+	Chr4:54392692-54392711	MsG0480021150.01.T01:intron	40.0%
	ACTGTTGATACAACTGGTGC+TGG	+	Chr4:54394793-54394812	MsG0480021150.01.T01:CDS	45.0%
	ATCAGCACATCTGAAGGCAA+TGG	+	Chr4:54393741-54393760	MsG0480021150.01.T01:CDS	45.0%
	ATGCAGATCAGCACATCTGA+AGG	+	Chr4:54393735-54393754	MsG0480021150.01.T01:CDS	45.0%
	CAATCTAAATGCAGAGAGGG+AGG	-	Chr4:54394611-54394630	None:intergenic	45.0%
	CTAAGGACATCAGCTTCTGT+TGG	-	Chr4:54395086-54395105	None:intergenic	45.0%
	CTCACAACATCCCAAACCAA+TGG	+	Chr4:54391942-54391961	MsG0480021150.01.T01:exon	45.0%
	GAAAACAACGTGAGAGGTGA+TGG	+	Chr4:54392210-54392229	MsG0480021150.01.T01:CDS	45.0%
	GAAGGCAATGGAAGTTGCTA+AGG	+	Chr4:54393753-54393772	MsG0480021150.01.T01:CDS	45.0%
	GGTCTATCTAGATCCACCTA+GGG	-	Chr4:54392531-54392550	None:intergenic	45.0%
	GTGAAGTAGGCAAGTGGTTT+GGG	+	Chr4:54392714-54392733	MsG0480021150.01.T01:intron	45.0%
	GTGGAGCTGGAATTTCTAAC+TGG	+	Chr4:54393898-54393917	MsG0480021150.01.T01:CDS	45.0%
	GTTTCACGCGCGTGTTTAAT+TGG	+	Chr4:54392472-54392491	MsG0480021150.01.T01:intron	45.0%
	TAGCCCTACTATATCATGCG+CGG	+	Chr4:54393266-54393285	MsG0480021150.01.T01:intron	45.0%
	TCTCATCAAGGTCAGTGATG+TGG	+	Chr4:54393879-54393898	MsG0480021150.01.T01:CDS	45.0%
	TGGTCTATCTAGATCCACCT+AGG	-	Chr4:54392532-54392551	None:intergenic	45.0%
	TGGTTAATCCCACACATTGG+AGG	+	Chr4:54393344-54393363	MsG0480021150.01.T01:intron	45.0%
!	AATTGGTCTAGGTCACTAGG+TGG	+	Chr4:54393418-54393437	MsG0480021150.01.T01:intron	45.0%
!	ACTTCAAATTAGGGTGCCAC+AGG	-	Chr4:54393957-54393976	None:intergenic	45.0%
!	AGCTGGAATTGCTCCCTTTT+TGG	-	Chr4:54395060-54395079	None:intergenic	45.0%
!	ATTGGTCTAGGTCACTAGGT+GGG	+	Chr4:54393419-54393438	MsG0480021150.01.T01:intron	45.0%
!	CTTCAAATTAGGGTGCCACA+GGG	-	Chr4:54393956-54393975	None:intergenic	45.0%
!	GAGTCAGATTTTCCATGTGG+TGG	+	Chr4:54392503-54392522	MsG0480021150.01.T01:intron	45.0%
!	GCTGAGTCAGATTTTCCATG+TGG	+	Chr4:54392500-54392519	MsG0480021150.01.T01:intron	45.0%
!	TCACCCTTACATTTTTCCGC+TGG	+	Chr4:54395177-54395196	MsG0480021150.01.T01:three_prime_UTR	45.0%
!	TCACGTGTTTTCCTTGCTCA+GGG	+	Chr4:54392666-54392685	MsG0480021150.01.T01:intron	45.0%
!	TTCAGATGTGCTGATCTGCA+TGG	-	Chr4:54393736-54393755	None:intergenic	45.0%
!!	TGGCTTTAGTGGACTTCTCA+TGG	+	Chr4:54394552-54394571	MsG0480021150.01.T01:intron	45.0%
!!	ATAAAAGAATAAAAAAAAAA+AGG	-	Chr4:54393325-54393344	None:intergenic	5.0%
!!	TATATTTATTTATCTAAAAA+AGG	-	Chr4:54392778-54392797	None:intergenic	5.0%
!!!	TTTTTTTTTTTATTCTTTTA+TGG	+	Chr4:54393324-54393343	MsG0480021150.01.T01:intron	5.0%
	ATGGAAGTTGCTAAGGAGGC+AGG	+	Chr4:54393760-54393779	MsG0480021150.01.T01:CDS	50.0%
	ATTTGCTTACGGGCTTCCTC+TGG	-	Chr4:54393835-54393854	None:intergenic	50.0%
	CATGAACTCACGCTCTCCAT+CGG	-	Chr4:54392289-54392308	None:intergenic	50.0%
	CCCACACATTGGAGGAATTG+CGG	+	Chr4:54393352-54393371	MsG0480021150.01.T01:intron	50.0%
	CCGCAATTCCTCCAATGTGT+GGG	-	Chr4:54393355-54393374	None:intergenic	50.0%
	CTCACACGCGCATACAAAAC+AGG	-	Chr4:54393609-54393628	None:intergenic	50.0%
	GGACAAGGCTGATCTCATCA+AGG	+	Chr4:54393867-54393886	MsG0480021150.01.T01:CDS	50.0%
	GGCAATGGAAGTTGCTAAGG+AGG	+	Chr4:54393756-54393775	MsG0480021150.01.T01:CDS	50.0%
	GGTGAAGTAGGCAAGTGGTT+TGG	+	Chr4:54392713-54392732	MsG0480021150.01.T01:intron	50.0%
	GTCCACTAAAGCCATAGCAC+AGG	-	Chr4:54394546-54394565	None:intergenic	50.0%
	TCAGCTTCTGTTGGAAGAGC+TGG	-	Chr4:54395077-54395096	None:intergenic	50.0%
	TCCGCAATTCCTCCAATGTG+TGG	-	Chr4:54393356-54393375	None:intergenic	50.0%
	TCCTTCGTTGGAGCTCTGTT+GGG	+	Chr4:54394820-54394839	MsG0480021150.01.T01:CDS	50.0%
	TCTGCCTTCGTCGGAAAACT+CGG	+	Chr4:54392147-54392166	MsG0480021150.01.T01:CDS	50.0%
	TTCCTTCGTTGGAGCTCTGT+TGG	+	Chr4:54394819-54394838	MsG0480021150.01.T01:CDS	50.0%
	TTGTATGCGCGTGTGAGTGA+GGG	+	Chr4:54393612-54393631	MsG0480021150.01.T01:intron	50.0%
	TTTGTATGCGCGTGTGAGTG+AGG	+	Chr4:54393611-54393630	MsG0480021150.01.T01:intron	50.0%
	TTTGTTACTCTACGCGCCGA+TGG	+	Chr4:54392270-54392289	MsG0480021150.01.T01:CDS	50.0%
!	ATCACCGAGTTTTCCGACGA+AGG	-	Chr4:54392154-54392173	None:intergenic	50.0%
!	GTCACGTGTTTTCCTTGCTC+AGG	+	Chr4:54392665-54392684	MsG0480021150.01.T01:intron	50.0%
!!	CACCTGTGCTATGGCTTTAG+TGG	+	Chr4:54394541-54394560	MsG0480021150.01.T01:intron	50.0%
	AATTCGGTCACATGCTCGCC+GGG	+	Chr4:54392178-54392197	MsG0480021150.01.T01:CDS	55.0%
	ACCTTTGTGGCCTTCACCAG+AGG	+	Chr4:54393816-54393835	MsG0480021150.01.T01:CDS	55.0%
	AGGGAGACACCAGAGGTTGT+TGG	-	Chr4:54392045-54392064	None:intergenic	55.0%
	ATGCTTCTGCTCTCTCCCTG+TGG	+	Chr4:54393938-54393957	MsG0480021150.01.T01:CDS	55.0%
	CAAACCTCCGGCTACCTTTG+TGG	+	Chr4:54393803-54393822	MsG0480021150.01.T01:CDS	55.0%
	CAAGGTCAGTGATGTGGAGC+TGG	+	Chr4:54393885-54393904	MsG0480021150.01.T01:CDS	55.0%
	CACAAAGGTAGCCGGAGGTT+TGG	-	Chr4:54393805-54393824	None:intergenic	55.0%
	CGCTGGAATCACCAACCGTT+TGG	-	Chr4:54393476-54393495	None:intergenic	55.0%
	CTAGGTCACTAGGTGGGTAG+TGG	+	Chr4:54393425-54393444	MsG0480021150.01.T01:intron	55.0%
	GAAACCGGGAGCTTCAGCAA+GGG	-	Chr4:54392064-54392083	None:intergenic	55.0%
	GAATTCGGTCACATGCTCGC+CGG	+	Chr4:54392177-54392196	MsG0480021150.01.T01:CDS	55.0%
	GACTTCGTCCCAACAACCTC+TGG	+	Chr4:54392033-54392052	MsG0480021150.01.T01:CDS	55.0%
	GGCGGTAAATCTGCCTTCGT+CGG	+	Chr4:54392138-54392157	MsG0480021150.01.T01:CDS	55.0%
	GGGAGACACCAGAGGTTGTT+GGG	-	Chr4:54392044-54392063	None:intergenic	55.0%
	GGGGTGTGTTTGTCACGTGA+GGG	+	Chr4:54392640-54392659	MsG0480021150.01.T01:intron	55.0%
	GTGAAGGCCACAAAGGTAGC+CGG	-	Chr4:54393813-54393832	None:intergenic	55.0%
	TCCGGTCTCATCGTCAGCTT+CGG	+	Chr4:54391997-54392016	MsG0480021150.01.T01:CDS	55.0%
	TCCTCTGGTGAAGGCCACAA+AGG	-	Chr4:54393820-54393839	None:intergenic	55.0%
	TTACGGGCTTCCTCTGGTGA+AGG	-	Chr4:54393829-54393848	None:intergenic	55.0%
	TTCAGCAAGGGAGACACCAG+AGG	-	Chr4:54392052-54392071	None:intergenic	55.0%
!	AGCGTTGGAGGCCATTGGTT+TGG	-	Chr4:54391956-54391975	None:intergenic	55.0%
!	ATCGCTGTTTCACGACTCGG+CGG	+	Chr4:54392120-54392139	MsG0480021150.01.T01:CDS	55.0%
!	GCGATCGCTGTTTCACGACT+CGG	+	Chr4:54392117-54392136	MsG0480021150.01.T01:CDS	55.0%
!	GTTTTCCTTGCTCAGGGCTG+TGG	+	Chr4:54392672-54392691	MsG0480021150.01.T01:intron	55.0%
!!	GCGTTGGAGGCCATTGGTTT+GGG	-	Chr4:54391955-54391974	None:intergenic	55.0%
!!	GGTGCTGGTGATTCCTTCGT+TGG	+	Chr4:54394808-54394827	MsG0480021150.01.T01:CDS	55.0%
!!	TTTCCATGTGGTGGGTCCCT+AGG	+	Chr4:54392512-54392531	MsG0480021150.01.T01:intron	55.0%
	AAGGCCACAAAGGTAGCCGG+AGG	-	Chr4:54393810-54393829	None:intergenic	60.0%
	ACAGCGATCGCGACGTTAGC+TGG	-	Chr4:54392108-54392127	None:intergenic	60.0%
	ACGTTAGCTGGTGCACCACC+GGG	-	Chr4:54392096-54392115	None:intergenic	60.0%
	GACGTTAGCTGGTGCACCAC+CGG	-	Chr4:54392097-54392116	None:intergenic	60.0%
	GCCCAACAGAGCTCCAACGA+AGG	-	Chr4:54394824-54394843	None:intergenic	60.0%
	GCCGAAGCTGACGATGAGAC+CGG	-	Chr4:54392001-54392020	None:intergenic	60.0%
	GGAAACCGGGAGCTTCAGCA+AGG	-	Chr4:54392065-54392084	None:intergenic	60.0%
	GTCTCCCTTGCTGAAGCTCC+CGG	+	Chr4:54392057-54392076	MsG0480021150.01.T01:CDS	60.0%
!	CACTAGGTGGGTAGTGGTGG+TGG	+	Chr4:54393431-54393450	MsG0480021150.01.T01:intron	60.0%
!	GGGGGTGTGTTTGTCACGTG+AGG	+	Chr4:54392639-54392658	MsG0480021150.01.T01:intron	60.0%
!	GGTCACTAGGTGGGTAGTGG+TGG	+	Chr4:54393428-54393447	MsG0480021150.01.T01:intron	60.0%
!!	ACCGGGGGCTTTGAGGAAAC+CGG	-	Chr4:54392079-54392098	None:intergenic	60.0%
!!	AGGGAAGCGTTGGAGGCCAT+TGG	-	Chr4:54391961-54391980	None:intergenic	60.0%
!!	CAAAGGCGCTCGTACTGCAC+TGG	+	Chr4:54392245-54392264	MsG0480021150.01.T01:CDS	60.0%
!!	TAGGTGGGTAGTGGTGGTGG+TGG	+	Chr4:54393434-54393453	MsG0480021150.01.T01:intron	60.0%
	CCACCTAGGGACCCACCACA+TGG	-	Chr4:54392518-54392537	None:intergenic	65.0%
	CCCGGTTTCCTCAAAGCCCC+CGG	+	Chr4:54392075-54392094	MsG0480021150.01.T01:CDS	65.0%
	CGTTAGCTGGTGCACCACCG+GGG	-	Chr4:54392095-54392114	None:intergenic	65.0%
	GCTAGCCACAGCCCTGAGCA+AGG	-	Chr4:54392680-54392699	None:intergenic	65.0%
	GCTGACGATGAGACCGGAGC+CGG	-	Chr4:54391995-54392014	None:intergenic	65.0%
	GGTTTCCTCAAAGCCCCCGG+TGG	+	Chr4:54392078-54392097	MsG0480021150.01.T01:CDS	65.0%
	GTTAGCTGGTGCACCACCGG+GGG	-	Chr4:54392094-54392113	None:intergenic	65.0%
!!	CCATGTGGTGGGTCCCTAGG+TGG	+	Chr4:54392515-54392534	MsG0480021150.01.T01:intron	65.0%
!!	CCGGGGGCTTTGAGGAAACC+GGG	-	Chr4:54392078-54392097	None:intergenic	65.0%
	ATGAGACCGGAGCCGGTGGC+GGG	-	Chr4:54391988-54392007	None:intergenic	70.0%
	GACGATGAGACCGGAGCCGG+TGG	-	Chr4:54391992-54392011	None:intergenic	70.0%
	GATGAGACCGGAGCCGGTGG+CGG	-	Chr4:54391989-54392008	None:intergenic	70.0%
	GTGCACCACCGGGGGCTTTG+AGG	-	Chr4:54392086-54392105	None:intergenic	70.0%
!!	GGGGAGGAGGGAAGCGTTGG+AGG	-	Chr4:54391968-54391987	None:intergenic	70.0%
	GCTTCCCTCCTCCCCGCCAC+CGG	+	Chr4:54391973-54391992	MsG0480021150.01.T01:CDS	75.0%
	TGAGACCGGAGCCGGTGGCG+GGG	-	Chr4:54391987-54392006	None:intergenic	75.0%
!	GGCGGGGAGGAGGGAAGCGT+TGG	-	Chr4:54391971-54391990	None:intergenic	75.0%
!!!	CTCCTCCCCGCCACCGGCTC+CGG	+	Chr4:54391979-54391998	MsG0480021150.01.T01:CDS	80.0%
!!!	GGAGCCGGTGGCGGGGAGGA+GGG	-	Chr4:54391980-54391999	None:intergenic	80.0%
!!!	CGGAGCCGGTGGCGGGGAGG+AGG	-	Chr4:54391981-54392000	None:intergenic	85.0%
!!!	GACCGGAGCCGGTGGCGGGG+AGG	-	Chr4:54391984-54392003	None:intergenic	85.0%

Chromosome	Type	Strat	End	Strand	Name
Chr4	gene	54391756	54395378	54391756	ID=MsG0480021150.01;Name=MsG0480021150.01
Chr4	mRNA	54391756	54395378	54391756	ID=MsG0480021150.01.T01;Parent=MsG0480021150.01;Name=MsG0480021150.01.T01;_AED=0.30;_eAED=0.31;_QI=205\|0.66\|0.75\|1\|1\|1\|4\|254\|343
Chr4	exon	54391756	54392377	54391756	ID=MsG0480021150.01.T01:exon:20181;Parent=MsG0480021150.01.T01
Chr4	exon	54393691	54394023	54393691	ID=MsG0480021150.01.T01:exon:20182;Parent=MsG0480021150.01.T01
Chr4	exon	54394721	54394873	54394721	ID=MsG0480021150.01.T01:exon:20183;Parent=MsG0480021150.01.T01
Chr4	exon	54394996	54395378	54394996	ID=MsG0480021150.01.T01:exon:20184;Parent=MsG0480021150.01.T01
Chr4	five_prime_UTR	54391756	54391960	54391756	ID=MsG0480021150.01.T01:five_prime_utr;Parent=MsG0480021150.01.T01
Chr4	CDS	54391961	54392377	54391961	ID=MsG0480021150.01.T01:cds;Parent=MsG0480021150.01.T01
Chr4	CDS	54393691	54394023	54393691	ID=MsG0480021150.01.T01:cds;Parent=MsG0480021150.01.T01
Chr4	CDS	54394721	54394873	54394721	ID=MsG0480021150.01.T01:cds;Parent=MsG0480021150.01.T01
Chr4	CDS	54394996	54395124	54394996	ID=MsG0480021150.01.T01:cds;Parent=MsG0480021150.01.T01
Chr4	three_prime_UTR	54395125	54395378	54395125	ID=MsG0480021150.01.T01:three_prime_utr;Parent=MsG0480021150.01.T01

Gene Sequence

>MsG0480021150.01.T01

ATGGCCTCCAACGCTTCCCTCCTCCCCGCCACCGGCTCCGGTCTCATCGTCAGCTTCGGCGAGATGCTCATTGACTTCGTCCCAACAACCTCTGGTGTCTCCCTTGCTGAAGCTCCCGGTTTCCTCAAAGCCCCCGGTGGTGCACCAGCTAACGTCGCGATCGCTGTTTCACGACTCGGCGGTAAATCTGCCTTCGTCGGAAAACTCGGTGATGATGAATTCGGTCACATGCTCGCCGGGATCTTGAAAGAAAACAACGTGAGAGGTGATGGTATCAACTTTGACAAAGGCGCTCGTACTGCACTGGCGTTTGTTACTCTACGCGCCGATGGAGAGCGTGAGTTCATGTTTTACAGAAATCCGAGTGCTGATATGCTTCTTACTCCTGAAGATCTCAATCTTGAACTCATCAGATCTGCAAAAGTCTTCCATTATGGATCGATAAGTTTGATTGTGGAGCCATGCAGATCAGCACATCTGAAGGCAATGGAAGTTGCTAAGGAGGCAGGTTGCTTACTCTCTTATGATCCAAACCTCCGGCTACCTTTGTGGCCTTCACCAGAGGAAGCCCGTAAGCAAATTCTCAGTATATGGGACAAGGCTGATCTCATCAAGGTCAGTGATGTGGAGCTGGAATTTCTAACTGGAAGTGATAAAATTGATGATGCTTCTGCTCTCTCCCTGTGGCACCCTAATTTGAAGTTACTTCTTGTAACTCTTGGTGAAAATGGTTCCAGATATTATACAAAGTTTGTGACAATTTTTCTCCTTTTCTGGACAAATCAGAATTTCAAAGGATCAGTAGATGCTTTCCATGTCAACACTGTTGATACAACTGGTGCTGGTGATTCCTTCGTTGGAGCTCTGTTGGGCAAGATTGTAGATGATCAATCCATACTTGAAGATGAAGCAAGGTTAAGGGAAGTACTTAAATTTGCCAATGCATGTGGAGCAATTACTACTACCAAAAAGGGAGCAATTCCAGCTCTTCCAACAGAAGCTGATGTCCTTAGCCTGATCAAAAAGGCATAG

Protein sequence

>MsG0480021150.01.T01

MASNASLLPATGSGLIVSFGEMLIDFVPTTSGVSLAEAPGFLKAPGGAPANVAIAVSRLGGKSAFVGKLGDDEFGHMLAGILKENNVRGDGINFDKGARTALAFVTLRADGEREFMFYRNPSADMLLTPEDLNLELIRSAKVFHYGSISLIVEPCRSAHLKAMEVAKEAGCLLSYDPNLRLPLWPSPEEARKQILSIWDKADLIKVSDVELEFLTGSDKIDDASALSLWHPNLKLLLVTLGENGSRYYTKFVTIFLLFWTNQNFKGSVDAFHVNTVDTTGAGDSFVGALLGKIVDDQSILEDEARLREVLKFANACGAITTTKKGAIPALPTEADVLSLIKKA*