AlfalfaGEDB | MsG0180003726.01

M. sativa cultivar ZhongmuNo.1 / MsG0180003726.01

KEGG: K05765
GO: GO:0003779 GO:0005622 GO:0015629 GO:0030042
PFAM: PF00241

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180003726.01.T01	XP_013468343.1	100	138	0	0	40	177	2	139	2.21E-96	285

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180003726.01.T01	Q9ZSK3	84.058	138	22	0	40	177	2	139	4.20E-86	252

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180003726.01.T01	G7ZZK8	100.000	138	0	0	40	177	2	139	1.06e-96	285

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI

Gene1	Gene2	Type
MsG0180003726.01	MsG0780040739.01	PPI
MsG0180003726.01	MsG0180005439.01	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180003726.01.T01	MTR_1g068950	100.000	138	0	0	40	177	2	139	2.68e-100	285
MsG0180003726.01.T01	MTR_2g028670	80.435	138	27	0	40	177	2	139	1.07e-83	243
MsG0180003726.01.T01	MTR_8g088210	78.261	138	30	0	40	177	2	139	2.04e-80	235
MsG0180003726.01.T01	MTR_5g010430	75.714	140	32	1	37	176	1	138	2.65e-78	229
MsG0180003726.01.T01	MTR_8g088210	77.273	132	30	0	46	177	1	132	1.64e-75	222
MsG0180003726.01.T01	MTR_8g098470	73.723	137	34	1	40	176	2	136	2.36e-75	222
MsG0180003726.01.T01	MTR_1g101190	73.913	138	34	1	38	175	461	596	5.80e-69	220
MsG0180003726.01.T01	MTR_7g096890	60.284	141	56	0	37	177	6	146	8.39e-67	201
MsG0180003726.01.T01	MTR_4g073590	60.741	135	53	0	42	176	8	142	8.02e-60	183
MsG0180003726.01.T01	MTR_1g076170	62.319	138	52	0	40	177	10	147	2.72e-59	182
MsG0180003726.01.T01	MTR_4g073590	62.069	87	33	0	42	128	8	94	2.09e-37	125

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180003726.01.T01	AT5G59890	84.058	138	22	0	40	177	2	139	4.28e-87	252
MsG0180003726.01.T01	AT3G46010	84.058	138	22	0	40	177	13	150	1.63e-86	251
MsG0180003726.01.T01	AT3G46010	84.058	138	22	0	40	177	2	139	5.04e-86	249
MsG0180003726.01.T01	AT5G59890	83.333	132	22	0	46	177	1	132	3.53e-82	239
MsG0180003726.01.T01	AT5G59890	83.333	132	22	0	46	177	1	132	3.53e-82	239
MsG0180003726.01.T01	AT5G59880	76.812	138	32	0	40	177	2	139	1.87e-80	235
MsG0180003726.01.T01	AT1G01750	73.611	144	38	0	34	177	1	144	3.66e-80	234
MsG0180003726.01.T01	AT4G00680	73.611	144	37	1	34	177	7	149	7.01e-80	234
MsG0180003726.01.T01	AT4G00680	74.638	138	35	0	40	177	2	139	5.91e-79	231
MsG0180003726.01.T01	AT1G01750	75.362	138	34	0	40	177	2	139	1.79e-78	230
MsG0180003726.01.T01	AT3G46000	78.261	138	28	1	40	177	2	137	2.74e-78	229
MsG0180003726.01.T01	AT3G46000	78.261	138	28	1	40	177	21	156	4.28e-78	229
MsG0180003726.01.T01	AT4G25590	75.912	137	31	1	40	176	2	136	3.75e-75	221
MsG0180003726.01.T01	AT3G46000	77.273	132	28	1	46	177	1	130	1.60e-73	217
MsG0180003726.01.T01	AT5G52360	75.912	137	31	1	40	176	2	136	5.41e-73	216
MsG0180003726.01.T01	AT4G25590	74.809	131	31	1	46	176	1	129	2.44e-70	209
MsG0180003726.01.T01	AT5G59880	65.942	138	32	1	40	177	2	124	2.66e-65	196
MsG0180003726.01.T01	AT2G31200	58.865	141	58	0	37	177	6	146	3.44e-63	192
MsG0180003726.01.T01	AT4G34970	54.745	137	62	0	41	177	5	141	3.57e-56	174
MsG0180003726.01.T01	AT2G16700	55.147	136	61	0	42	177	8	143	6.15e-56	173
MsG0180003726.01.T01	AT2G16700	55.303	132	59	0	46	177	1	132	1.03e-53	167
MsG0180003726.01.T01	AT2G16700	55.303	132	59	0	46	177	1	132	1.03e-53	167
MsG0180003726.01.T01	AT3G45990	52.174	138	55	3	46	177	1	133	6.99e-45	145
MsG0180003726.01.T01	AT2G16700	50.980	102	50	0	42	143	8	109	1.59e-34	118

Find 31 sgRNAs with CRISPR-Local

Find 40 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
CTCCTTACCCTTGAAATATC+AGG	0.195342	1:-67354917	None:intergenic
CAGATGCATCAACAAATATT+TGG	0.287475	1:-67354492	None:intergenic
TACACTAATCCAATGGTAAT+GGG	0.332781	1:+67354406	None:intergenic
CTACACTAATCCAATGGTAA+TGG	0.421646	1:+67354405	None:intergenic
TTTATGCTAGCTCCAAAGAT+AGG	0.430442	1:+67354948	MsG0180003726.01.T01:CDS
AAATCATCATAACCATTTGC+AGG	0.445223	1:-67354676	None:intergenic
GAAGGTGCCCTGATATTTCA+AGG	0.462729	1:+67354909	MsG0180003726.01.T01:intron
TTACAGGCGAATGCAGCATC+AGG	0.463408	1:+67354529	MsG0180003726.01.T01:CDS
AATATTTGGTAATTAACAAA+AGG	0.468681	1:-67354478	None:intergenic
GCTCCAAAGATAGGTTCAAA+AGG	0.469123	1:+67354957	MsG0180003726.01.T01:CDS
TGTGGCGTTGAAAGTGTGTA+TGG	0.483413	1:+67354447	MsG0180003726.01.T01:CDS
GGTTCAAAAGGGAGCTTGAT+GGG	0.491075	1:+67354969	MsG0180003726.01.T01:CDS
ACTAACAACCCCATTACCAT+TGG	0.500190	1:-67354415	None:intergenic
GCCCTGATATTTCAAGGGTA+AGG	0.520257	1:+67354915	MsG0180003726.01.T01:CDS
GCATATCGACACTCGTCAGC+AGG	0.527187	1:-67354712	None:intergenic
GGCGAATGCAGCATCAGGTA+TGG	0.530905	1:+67354534	MsG0180003726.01.T01:CDS
CAAGTTGTTGTTGACAAGGT+TGG	0.532386	1:+67354649	MsG0180003726.01.T01:CDS
CTCCAAAGATAGGTTCAAAA+GGG	0.546134	1:+67354958	MsG0180003726.01.T01:CDS
AGCTACTGATCCTACTGAGA+TGG	0.549628	1:+67355009	MsG0180003726.01.T01:CDS
AGGTTCAAAAGGGAGCTTGA+TGG	0.551429	1:+67354968	MsG0180003726.01.T01:CDS
GTTGTTAGTATGCTTGTTTG+TGG	0.553370	1:+67354429	MsG0180003726.01.T01:CDS
TCCTTACCCTTGAAATATCA+GGG	0.558692	1:-67354916	None:intergenic
AAGGTTGGTGATCCTGCAAA+TGG	0.580318	1:+67354664	MsG0180003726.01.T01:CDS
GAAGCAAGTTGTTGTTGACA+AGG	0.580658	1:+67354645	MsG0180003726.01.T01:CDS
AAGGTGCCCTGATATTTCAA+GGG	0.592018	1:+67354910	MsG0180003726.01.T01:intron
CTTGTAAATGATATACCGAT+AGG	0.592223	1:-67354608	None:intergenic
ACATCAAGATCCATCTCAGT+AGG	0.597944	1:-67355019	None:intergenic
TCAAGGCGAAGAGGACCTAT+CGG	0.606898	1:+67354593	MsG0180003726.01.T01:CDS
ACACTAATCCAATGGTAATG+GGG	0.640777	1:+67354407	None:intergenic
GTATATCATTTACAAGATCG+AGG	0.668947	1:+67354615	MsG0180003726.01.T01:CDS
TCACAAAAGATTAATTGACA+CGG	0.694452	1:-67355051	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TTAAAATCAATCATAAAAAA+TGG	-	Chr1:67354859-67354878	None:intergenic	10.0%
!!!	AATATTTGGTAATTAACAAA+AGG	-	Chr1:67354481-67354500	None:intergenic	15.0%
!	AAGCAACAAAATGATATACT+CGG	-	Chr1:67354883-67354902	None:intergenic	25.0%
!	CAACAGTTAACTTATAGATT+TGG	+	Chr1:67354828-67354847	MsG0180003726.01.T01:intron	25.0%
!!	TGAATGCAAGTTAAAGTTTT+TGG	+	Chr1:67354567-67354586	MsG0180003726.01.T01:CDS	25.0%
!!	TGATGTTTGTGAAATTTTAC+AGG	+	Chr1:67354513-67354532	MsG0180003726.01.T01:CDS	25.0%
!!!	ATTTTGTTGCTTTTGTTTGA+AGG	+	Chr1:67354891-67354910	MsG0180003726.01.T01:intron	25.0%
	AAATCATCATAACCATTTGC+AGG	-	Chr1:67354679-67354698	None:intergenic	30.0%
	AGCAACAAAATGATATACTC+GGG	-	Chr1:67354882-67354901	None:intergenic	30.0%
	CAGATGCATCAACAAATATT+TGG	-	Chr1:67354495-67354514	None:intergenic	30.0%
	CTTGTAAATGATATACCGAT+AGG	-	Chr1:67354611-67354630	None:intergenic	30.0%
	GTATATCATTTACAAGATCG+AGG	+	Chr1:67354615-67354634	MsG0180003726.01.T01:CDS	30.0%
!!	TGAAGAAAATTCTGCTTTTC+TGG	-	Chr1:67354774-67354793	None:intergenic	30.0%
!!!	TGATTTTGATTTTGTCACTG+AGG	+	Chr1:67354741-67354760	MsG0180003726.01.T01:CDS	30.0%
	CTCCAAAGATAGGTTCAAAA+GGG	+	Chr1:67354958-67354977	MsG0180003726.01.T01:CDS	35.0%
	GTTGTTAGTATGCTTGTTTG+TGG	+	Chr1:67354429-67354448	MsG0180003726.01.T01:CDS	35.0%
	TCCTTACCCTTGAAATATCA+GGG	-	Chr1:67354919-67354938	None:intergenic	35.0%
	TTTATGCTAGCTCCAAAGAT+AGG	+	Chr1:67354948-67354967	MsG0180003726.01.T01:CDS	35.0%
!	GCAGAATTTTCTTCATAGCT+TGG	+	Chr1:67354779-67354798	MsG0180003726.01.T01:CDS	35.0%
!!!	GCTTGGTAATGTCAACTTTT+TGG	+	Chr1:67354796-67354815	MsG0180003726.01.T01:intron	35.0%
!!!	GTTAAAGTTTTTGGAGCTCA+AGG	+	Chr1:67354576-67354595	MsG0180003726.01.T01:CDS	35.0%
	ACATCAAGATCCATCTCAGT+AGG	-	Chr1:67355022-67355041	None:intergenic	40.0%
	CTCCTTACCCTTGAAATATC+AGG	-	Chr1:67354920-67354939	None:intergenic	40.0%
	GCTCCAAAGATAGGTTCAAA+AGG	+	Chr1:67354957-67354976	MsG0180003726.01.T01:CDS	40.0%
!	AAGGTGCCCTGATATTTCAA+GGG	+	Chr1:67354910-67354929	MsG0180003726.01.T01:intron	40.0%
!	CAAGTTGTTGTTGACAAGGT+TGG	+	Chr1:67354649-67354668	MsG0180003726.01.T01:CDS	40.0%
!	CTCCCTTTTGAACCTATCTT+TGG	-	Chr1:67354963-67354982	None:intergenic	40.0%
!	GAAGCAAGTTGTTGTTGACA+AGG	+	Chr1:67354645-67354664	MsG0180003726.01.T01:CDS	40.0%
	AGCTACTGATCCTACTGAGA+TGG	+	Chr1:67355009-67355028	MsG0180003726.01.T01:CDS	45.0%
	AGGTTCAAAAGGGAGCTTGA+TGG	+	Chr1:67354968-67354987	MsG0180003726.01.T01:CDS	45.0%
	GCCCTGATATTTCAAGGGTA+AGG	+	Chr1:67354915-67354934	MsG0180003726.01.T01:CDS	45.0%
	GGTTCAAAAGGGAGCTTGAT+GGG	+	Chr1:67354969-67354988	MsG0180003726.01.T01:CDS	45.0%
!	TGTGGCGTTGAAAGTGTGTA+TGG	+	Chr1:67354447-67354466	MsG0180003726.01.T01:CDS	45.0%
!!	AAGGTTGGTGATCCTGCAAA+TGG	+	Chr1:67354664-67354683	MsG0180003726.01.T01:CDS	45.0%
!!	GAAGGTGCCCTGATATTTCA+AGG	+	Chr1:67354909-67354928	MsG0180003726.01.T01:intron	45.0%
	TCAAGGCGAAGAGGACCTAT+CGG	+	Chr1:67354593-67354612	MsG0180003726.01.T01:CDS	50.0%
	TTACAGGCGAATGCAGCATC+AGG	+	Chr1:67354529-67354548	MsG0180003726.01.T01:CDS	50.0%
!!!	TTTTGGAGCTCAAGGCGAAG+AGG	+	Chr1:67354584-67354603	MsG0180003726.01.T01:CDS	50.0%
	GCATATCGACACTCGTCAGC+AGG	-	Chr1:67354715-67354734	None:intergenic	55.0%
	GGCGAATGCAGCATCAGGTA+TGG	+	Chr1:67354534-67354553	MsG0180003726.01.T01:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
Chr1	gene	67354418	67355063	67354418	ID=MsG0180003726.01;Name=MsG0180003726.01
Chr1	mRNA	67354418	67355063	67354418	ID=MsG0180003726.01.T01;Parent=MsG0180003726.01;Name=MsG0180003726.01.T01;_AED=0.36;_eAED=0.50;_QI=0\|0\|0\|1\|1\|1\|2\|0\|177
Chr1	exon	67354418	67354800	67354418	ID=MsG0180003726.01.T01:exon:24042;Parent=MsG0180003726.01.T01
Chr1	exon	67354913	67355063	67354913	ID=MsG0180003726.01.T01:exon:24043;Parent=MsG0180003726.01.T01
Chr1	CDS	67354418	67354800	67354418	ID=MsG0180003726.01.T01:cds;Parent=MsG0180003726.01.T01
Chr1	CDS	67354913	67355063	67354913	ID=MsG0180003726.01.T01:cds;Parent=MsG0180003726.01.T01

Gene Sequence

>MsG0180003726.01.T01

ATGGTAATGGGGTTGTTAGTATGCTTGTTTGTGGCGTTGAAAGTGTGTATGGAGAAATTTCCTTTTGTTAATTACCAAATATTTGTTGATGCATCTGATGTTTGTGAAATTTTACAGGCGAATGCAGCATCAGGTATGGCTGTGAATGATGAATGCAAGTTAAAGTTTTTGGAGCTCAAGGCGAAGAGGACCTATCGGTATATCATTTACAAGATCGAGGAGAAGCAGAAGCAAGTTGTTGTTGACAAGGTTGGTGATCCTGCAAATGGTTATGATGATTTCACTGCTAATCTTCCTGCTGACGAGTGTCGATATGCTGTTTATGATTTTGATTTTGTCACTGAGGAGAATTGCCAGAAAAGCAGAATTTTCTTCATAGCTTGGTGCCCTGATATTTCAAGGGTAAGGAGCAAGATGATTTATGCTAGCTCCAAAGATAGGTTCAAAAGGGAGCTTGATGGGATTCAAGTTGAGTTGCAAGCTACTGATCCTACTGAGATGGATCTTGATGTGTTCAAAAGCCGTGTCAATTAA

Protein sequence

>MsG0180003726.01.T01

MVMGLLVCLFVALKVCMEKFPFVNYQIFVDASDVCEILQANAASGMAVNDECKLKFLELKAKRTYRYIIYKIEEKQKQVVVDKVGDPANGYDDFTANLPADECRYAVYDFDFVTEENCQKSRIFFIAWCPDISRVRSKMIYASSKDRFKRELDGIQVELQATDPTEMDLDVFKSRVN*