Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180003802.01.T01 | XP_013468456.1 | 90.789 | 456 | 32 | 2 | 1 | 456 | 1 | 446 | 0 | 754 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180003802.01.T01 | A0A072VM44 | 90.789 | 456 | 32 | 2 | 1 | 456 | 1 | 446 | 0.0 | 754 |
Co-expression Network
PPI
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180003802.01.T01 | MTR_1g069695 | 90.789 | 456 | 32 | 2 | 1 | 456 | 1 | 446 | 0.0 | 754 |
| MsG0180003802.01.T01 | MTR_1g069675 | 68.421 | 133 | 35 | 2 | 106 | 238 | 2 | 127 | 9.49e-55 | 179 |
| MsG0180003802.01.T01 | MTR_2g103940 | 32.921 | 404 | 207 | 13 | 25 | 398 | 59 | 428 | 1.11e-48 | 174 |
| MsG0180003802.01.T01 | MTR_2g019170 | 33.161 | 386 | 242 | 7 | 26 | 404 | 22 | 398 | 2.87e-47 | 169 |
| MsG0180003802.01.T01 | MTR_1g069665 | 50.820 | 183 | 82 | 4 | 7 | 181 | 3 | 185 | 1.98e-46 | 159 |
| MsG0180003802.01.T01 | MTR_1g069680 | 52.439 | 164 | 67 | 4 | 7 | 162 | 3 | 163 | 7.64e-43 | 150 |
| MsG0180003802.01.T01 | MTR_1g093900 | 33.073 | 384 | 235 | 11 | 29 | 398 | 10 | 385 | 2.80e-39 | 148 |
| MsG0180003802.01.T01 | MTR_8g076360 | 40.833 | 240 | 138 | 3 | 160 | 398 | 127 | 363 | 1.67e-31 | 124 |
| MsG0180003802.01.T01 | MTR_8g076360 | 32.130 | 277 | 154 | 5 | 127 | 402 | 71 | 314 | 8.97e-19 | 88.2 |
| MsG0180003802.01.T01 | MTR_8g047220 | 40.271 | 221 | 130 | 2 | 161 | 381 | 153 | 371 | 3.43e-31 | 127 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180003802.01.T01 | AT4G28560 | 56.658 | 383 | 156 | 5 | 31 | 403 | 34 | 416 | 1.20e-147 | 429 |
| MsG0180003802.01.T01 | AT1G13230 | 34.737 | 380 | 230 | 11 | 28 | 398 | 31 | 401 | 3.56e-51 | 179 |
| MsG0180003802.01.T01 | AT3G25670 | 32.454 | 379 | 240 | 9 | 28 | 398 | 27 | 397 | 3.24e-49 | 175 |
| MsG0180003802.01.T01 | AT1G68780 | 31.881 | 436 | 270 | 16 | 1 | 420 | 1 | 425 | 4.11e-46 | 166 |
| MsG0180003802.01.T01 | AT1G80080 | 31.010 | 416 | 224 | 13 | 26 | 412 | 46 | 427 | 7.03e-44 | 161 |
| MsG0180003802.01.T01 | AT2G42800 | 31.726 | 394 | 249 | 10 | 16 | 399 | 14 | 397 | 2.50e-42 | 156 |
| MsG0180003802.01.T01 | AT4G36180 | 38.710 | 217 | 129 | 3 | 164 | 379 | 327 | 540 | 3.27e-30 | 124 |
| MsG0180003802.01.T01 | AT4G36180 | 36.641 | 262 | 156 | 5 | 145 | 405 | 381 | 633 | 1.55e-27 | 117 |
| MsG0180003802.01.T01 | AT4G36180 | 33.948 | 271 | 146 | 7 | 165 | 406 | 472 | 738 | 1.75e-26 | 113 |
| MsG0180003802.01.T01 | AT4G36180 | 26.027 | 438 | 202 | 12 | 45 | 403 | 45 | 439 | 3.16e-18 | 88.2 |
Find 99 sgRNAs with CRISPR-Local
Find 135 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAATAAACTTCTTTCAATCT+GGG | 0.418400 | 1:+68460491 | None:intergenic |
| AAGCTTGATTTAAGCTACAA+TGG | 0.498895 | 1:-68460626 | MsG0180003802.01.T01:CDS |
| ACAAGCTCTTCAAGAGACGA+AGG | 0.623128 | 1:+68460932 | None:intergenic |
| AGAACAACAATTTGAGTGGT+AGG | 0.531797 | 1:-68460247 | MsG0180003802.01.T01:CDS |
| AGACTTGTCTTGATCGGAAA+TGG | 0.314047 | 1:-68460842 | MsG0180003802.01.T01:CDS |
| AGAGTGGCGTTAGAGGAACA+AGG | 0.744585 | 1:+68461058 | None:intergenic |
| AGCCCAAATTCCTTAGGAAC+AGG | 0.434530 | 1:+68460299 | None:intergenic |
| AGCTTGATTTAAGCTACAAT+GGG | 0.478764 | 1:-68460625 | MsG0180003802.01.T01:CDS |
| AGGAAATGTAGGGCTGTGTT+TGG | 0.396082 | 1:-68460183 | MsG0180003802.01.T01:CDS |
| AGGTGGGGTTGGTGTTTCAT+CGG | 0.542503 | 1:+68461078 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AAGCTTGATTTAAGCTACAA+TGG | - | Chr1:68460742-68460761 | MsG0180003802.01.T01:CDS | 30.0% | |
| AGCTTGATTTAAGCTACAAT+GGG | - | Chr1:68460743-68460762 | MsG0180003802.01.T01:CDS | 30.0% | |
| AGTTGGACAAAAGTTAAAGT+TGG | - | Chr1:68461161-68461180 | MsG0180003802.01.T01:CDS | 30.0% | |
| CGGTAAAATTCCAGAAATAT+GGG | - | Chr1:68460918-68460937 | MsG0180003802.01.T01:CDS | 30.0% | |
| CTAAGTCAGAATGAGTTTAA+TGG | - | Chr1:68460679-68460698 | MsG0180003802.01.T01:CDS | 30.0% | |
| GAATTTCTCCTAAGAACAAA+GGG | + | Chr1:68460858-68460877 | None:intergenic | 30.0% | |
| TCTGAAAATGAAGGTAGTTT+GGG | - | Chr1:68461228-68461247 | MsG0180003802.01.T01:CDS | 30.0% | |
| TTAAGTGGGAATTTACTTTC+CGG | - | Chr1:68460898-68460917 | MsG0180003802.01.T01:CDS | 30.0% | |
| AGAACAACAATTTGAGTGGT+AGG | - | Chr1:68461121-68461140 | MsG0180003802.01.T01:CDS | 35.0% | |
| ATCACTTTCTCAACTAGAGT+TGG | - | Chr1:68461144-68461163 | MsG0180003802.01.T01:CDS | 35.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr1 | gene | 68460010 | 68461380 | 68460010 | ID=MsG0180003802.01;Name=MsG0180003802.01 |
| Chr1 | mRNA | 68460010 | 68461380 | 68460010 | ID=MsG0180003802.01.T01;Parent=MsG0180003802.01;Name=MsG0180003802.01.T01;_AED=0.42;_eAED=0.42;_QI=0|-1|0|1|-1|1|1|0|456 |
| Chr1 | exon | 68460010 | 68461380 | 68460010 | ID=MsG0180003802.01.T01:exon:51887;Parent=MsG0180003802.01.T01 |
| Chr1 | CDS | 68460010 | 68461380 | 68460010 | ID=MsG0180003802.01.T01:cds;Parent=MsG0180003802.01.T01 |
| Chr1 | mRNA | 68460747 | 68460845 | 68460747 | ID=MsG0180003802.01.T02;Parent=MsG0180003802.01;Name=MsG0180003802.01.T02;_AED=0.49;_eAED=1.00;_QI=0|-1|0|1|-1|0|1|0|32 |
| Chr1 | exon | 68460747 | 68460845 | 68460747 | ID=MsG0180003802.01.T02:exon:51888;Parent=MsG0180003802.01.T02 |
| Chr1 | CDS | 68460747 | 68460845 | 68460747 | ID=MsG0180003802.01.T02:cds;Parent=MsG0180003802.01.T02 |
| Gene Sequence |
>MsG0180003802.01.T02 ATGGGTTCCACGCTGAACTTCCTTCGCAGATTGGTGATTTTTGTGACTTGGAAGAGCTTACTCTTTCCAAAAACAATCTCTCCGGTGAGGTTCCGGTGA >MsG0180003802.01.T01 ATGGCTCTATTTTCAACTTCAACAACACAAACACTATTATTATGCTTATACTCATTCATCTTCTTGTTCTCACTTTCACTCTCTCAAGACCAACCTCTCATTGACCCAAAAGAACAAACAGCACTCTACAATGTTCTCACTTCCCTCAACCCAACCATACCATGGCAAACACTCTTCCCCGATGATCTCTGTCTCTCCGCCCCACACGGCGTCGTTTGCGACTATGACCAAAACCAACCACAAAATGCACACATTGTTGAACTCAACTTCGGCTACGTTTCCGATGAAACACCAACCCCACCTTGTTCCTCTAACGCCACTCTCAACCCGCTTCTCTTCACCTCTTTCACCTACCTCCGAAAACTCTTCTTCTACAAATGCTTCAACAACACACAAAACATCATTAACATCTCTCTTCCTTCTTTCCCTTCGTCTCTTGAAGAGCTTGTGTTCATCGACAACCCTTCTATTGTTTCACCTCTCCAACCTTTTCTCACCAATCTTACCTCTCTCAGAAGACTTGTCTTGATCGGAAATGGGTTCCACGCTGAACTTCCTTCGCAGATTGGTGATTTTTGTGACTTGGAAGAGCTTACTCTTTCCAAAAACAATCTCTCCGGTGAGGTTCCGGTGAGTTTGGGAAAACTGAAGAAGCTGAAGATTCTTGATCTAAGTCAGAATGAGTTTAATGGGTGTGTTCCAGAACAGGTTGGGAATTTAACTTCTCTTTTGAAGCTTGATTTAAGCTACAATGGGTTTAGCTGTAAAATCCCAGAGAGTTTTAGCAACTTGAAAAAAATGGAATTTTTGGATTTAAGTTTCAACCTTTTTGGTAATTTTGGTGTCCCTTTGTTCTTAGGAGAAATTCCCAGATTGAAAGAAGTTTATTTAAGTGGGAATTTACTTTCCGGTAAAATTCCAGAAATATGGGAAAATCTTGGTGCTATTGAAAAAATTGGATTTTCTAAAATGGGGTTGGTTGGTAAAATTCCAGTGTCAATGGGGGTTTATTTGAAGAATTTATCTTATCTTGGGCTTGATAACAATAAGCTTGATGGGCCTGTTCCTAAGGAATTTGGGCTTTTGGAGTTTGCTAATGAGATCAATTTGGAGAACAACAATTTGAGTGGTAGGATCACTTTCTCAACTAGAGTTGGACAAAAGTTAAAGTTGGCAGGAAATGTAGGGCTGTGTTTGGGAAACAATAATAGTAGCTGTTCTGAAAATGAAGGTAGTTTGGGTCAACTTAATCCCTGCAAAATAACAGATGTTGTTTCTGATAGTGAAGTCCTTTTCAATGGGGCTTCTTTGCTGCTTTTTGATCCTATTAAGCTGGTTTTGGTCATGTTGTTTGTTTTCATGGGATTTTGA |
| Protein sequence |
>MsG0180003802.01.T02 MGSTLNFLRRLVIFVTWKSLLFPKTISPVRFR* >MsG0180003802.01.T01 MALFSTSTTQTLLLCLYSFIFLFSLSLSQDQPLIDPKEQTALYNVLTSLNPTIPWQTLFPDDLCLSAPHGVVCDYDQNQPQNAHIVELNFGYVSDETPTPPCSSNATLNPLLFTSFTYLRKLFFYKCFNNTQNIINISLPSFPSSLEELVFIDNPSIVSPLQPFLTNLTSLRRLVLIGNGFHAELPSQIGDFCDLEELTLSKNNLSGEVPVSLGKLKKLKILDLSQNEFNGCVPEQVGNLTSLLKLDLSYNGFSCKIPESFSNLKKMEFLDLSFNLFGNFGVPLFLGEIPRLKEVYLSGNLLSGKIPEIWENLGAIEKIGFSKMGLVGKIPVSMGVYLKNLSYLGLDNNKLDGPVPKEFGLLEFANEINLENNNLSGRITFSTRVGQKLKLAGNVGLCLGNNNSSCSENEGSLGQLNPCKITDVVSDSEVLFNGASLLLFDPIKLVLVMLFVFMGF* |
