AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0180003819.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180003819.01.T01 MTR_7g097030 95.122 41 2 0 12 52 143 183 1.47e-19 80.5
MsG0180003819.01.T01 MTR_1g069825 95.122 41 2 0 12 52 131 171 1.69e-19 80.9
MsG0180003819.01.T01 MTR_1g442860 87.805 41 5 0 12 52 144 184 1.66e-17 75.5
MsG0180003819.01.T01 MTR_1g075990 87.805 41 5 0 12 52 132 172 2.06e-17 74.7
MsG0180003819.01.T01 MTR_4g094428 64.516 62 16 1 12 73 125 180 2.52e-16 72.4
MsG0180003819.01.T01 MTR_5g072510 80.488 41 8 0 12 52 116 156 4.36e-16 71.6
MsG0180003819.01.T01 MTR_1g080210 73.171 41 11 0 12 52 119 159 5.77e-16 70.5
MsG0180003819.01.T01 MTR_3g031830 75.610 41 10 0 12 52 121 161 4.53e-15 68.9
MsG0180003819.01.T01 MTR_2g092950 70.732 41 12 0 12 52 102 142 4.56e-14 65.5
MsG0180003819.01.T01 MTR_2g076400 70.732 41 12 0 12 52 112 152 1.27e-13 64.3
MsG0180003819.01.T01 MTR_2g016110 68.293 41 13 0 12 52 114 154 3.73e-13 63.2
MsG0180003819.01.T01 MTR_4g079930 63.415 41 15 0 12 52 108 148 1.23e-12 61.6
MsG0180003819.01.T01 MTR_8g086350 68.293 41 13 0 12 52 110 150 2.55e-12 61.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180003819.01.T01 AT2G31160 92.683 41 3 0 12 52 133 173 2.38e-19 80.1
MsG0180003819.01.T01 AT3G23290 87.805 41 5 0 12 52 127 167 1.45e-17 75.1
MsG0180003819.01.T01 AT5G28490 78.049 41 9 0 12 52 104 144 1.31e-15 69.7
MsG0180003819.01.T01 AT3G04510 70.732 41 12 0 12 52 112 152 1.12e-14 67.8
MsG0180003819.01.T01 AT1G07090 73.171 41 11 0 12 52 110 150 1.37e-14 67.4
MsG0180003819.01.T01 AT2G42610 70.732 41 12 0 12 52 104 144 7.47e-14 65.1
MsG0180003819.01.T01 AT2G42610 70.732 41 12 0 12 52 104 144 7.47e-14 65.1
MsG0180003819.01.T01 AT1G78815 68.293 41 13 0 12 52 119 159 4.42e-12 60.8
MsG0180003819.01.T01 AT5G58500 69.048 42 12 1 12 52 98 139 2.70e-11 58.5

Find 44 sgRNAs with CRISPR-Local

Find 274 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCACCAAATGGATTTGTTTC+TGG 0.131233 1:+68715596 None:intergenic
TGTTCCAGTCACGGCGTTTC+TGG 0.158859 1:+68716325 None:intergenic
ATGCACTGATAGGTCGTTTA+AGG 0.159661 1:-68715646 MsG0180003819.01.T01:intron
CGCCGTGACTGGAACACTTT+TGG 0.268565 1:-68716318 MsG0180003819.01.T01:CDS
TCCTGTATCCCACTTGTTGC+CGG 0.342567 1:+68711981 None:intergenic
ATAACTAATACCTCTTGCTT+TGG 0.355096 1:+68715529 None:intergenic
CGTTCTGGTCAAGCTGCTTT+CGG 0.361286 1:+68712017 None:intergenic
TGCGGTTGACGGCGTAAGTT+TGG 0.365674 1:-68711909 MsG0180003819.01.T01:CDS
ACCAAGTATCCACCGAATCC+TGG 0.409753 1:+68711941 None:intergenic
CCCAGGATTCGGTGGATACT+TGG 0.409836 1:-68711942 MsG0180003819.01.T01:CDS
AGAACGGCGGGTTTCGCCTC+CGG 0.425664 1:-68712000 MsG0180003819.01.T01:CDS
TGCACTGATAGGTCGTTTAA+GGG 0.427076 1:-68715645 MsG0180003819.01.T01:intron
GGAGGCGAAACCCGCCGTTC+TGG 0.435283 1:+68712002 None:intergenic
TCCGGCAACAAGTGGGATAC+AGG 0.440987 1:-68711982 MsG0180003819.01.T01:CDS
ATACTTGGTGGCCGGCTTTG+CGG 0.444919 1:-68711927 MsG0180003819.01.T01:CDS
GTGGCCGGCTTTGCGGTTGA+CGG 0.446750 1:-68711920 MsG0180003819.01.T01:CDS
AAAACCAGAAACGCCGTGAC+TGG 0.451024 1:-68716329 MsG0180003819.01.T01:CDS
TGCTTTCGGTAACATGTTTG+TGG 0.464872 1:+68712031 None:intergenic
CACGACCAGTACCCAGGATT+CGG 0.466558 1:-68711953 MsG0180003819.01.T01:CDS
CTAACAGCTCTAGCACCAAA+TGG 0.473078 1:+68715584 None:intergenic
AAGCCAGAAACAAATCCATT+TGG 0.482999 1:-68715599 MsG0180003819.01.T01:CDS
TTCGGTGGATACTTGGTGGC+CGG 0.483659 1:-68711935 MsG0180003819.01.T01:CDS
GGTTGACGGCGTAAGTTTGG+CGG 0.509800 1:-68711906 MsG0180003819.01.T01:CDS
ATCCACCGAATCCTGGGTAC+TGG 0.512169 1:+68711948 None:intergenic
TACGCCGTCAACCGCAAAGC+CGG 0.514220 1:+68711916 None:intergenic
GTTTCGCCTCCGGCAACAAG+TGG 0.519406 1:-68711990 MsG0180003819.01.T01:CDS
CCAAGTATCCACCGAATCCT+GGG 0.535749 1:+68711942 None:intergenic
AGCAGCTTGACCAGAACGGC+GGG 0.536308 1:-68712012 MsG0180003819.01.T01:CDS
TTAGGCCAGATTCGGGTAGG+AGG 0.549511 1:-68712066 MsG0180003819.01.T01:intron
GTAACAAAGTTGACAATGTA+CGG 0.550655 1:-68711863 MsG0180003819.01.T01:CDS
GGCCAAAAGTGTTCCAGTCA+CGG 0.557625 1:+68716316 None:intergenic
GACCAGTACCCAGGATTCGG+TGG 0.580346 1:-68711950 MsG0180003819.01.T01:CDS
TGTTAGACTTTACCTTCGTG+AGG 0.586964 1:-68715567 MsG0180003819.01.T01:CDS
TAGGCCAGATTCGGGTAGGA+GGG 0.595882 1:-68712065 MsG0180003819.01.T01:intron
CCATTAGGCCAGATTCGGGT+AGG 0.603102 1:-68712069 MsG0180003819.01.T01:intron
GATCTTCAATCCAAAGCAAG+AGG 0.603866 1:-68715539 MsG0180003819.01.T01:intron
AGACAGCACGACCAGTACCC+AGG 0.606222 1:-68711959 MsG0180003819.01.T01:CDS
AGGATTCGGTGGATACTTGG+TGG 0.606918 1:-68711939 MsG0180003819.01.T01:CDS
CGAAAGCAGCTTGACCAGAA+CGG 0.607365 1:-68712016 MsG0180003819.01.T01:CDS
TTTCGGTAACATGTTTGTGG+TGG 0.611947 1:+68712034 None:intergenic
TTTCGCCTCCGGCAACAAGT+GGG 0.630108 1:-68711989 MsG0180003819.01.T01:CDS
TGTATCCCACTTGTTGCCGG+AGG 0.649239 1:+68711984 None:intergenic
AAGCAGCTTGACCAGAACGG+CGG 0.670993 1:-68712013 MsG0180003819.01.T01:CDS
GAAGATCACGAACCTCACGA+AGG 0.739048 1:+68715555 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATATATATAAATTTATAA+TGG + Chr1:68713876-68713895 None:intergenic 0.0%
!! AAAAAAAATATCGAAAAAAT+TGG + Chr1:68712207-68712226 None:intergenic 10.0%
!! ATAATGAAACATTTAATTAA+TGG - Chr1:68713059-68713078 MsG0180003819.01.T01:intron 10.0%
!! ATTTATGGATAAATATTTAT+AGG - Chr1:68713420-68713439 MsG0180003819.01.T01:intron 10.0%
!! TTAAATTGATTAATTATTGT+GGG - Chr1:68713598-68713617 MsG0180003819.01.T01:intron 10.0%
!!! AAAAATAGTCATTTTAAATA+GGG - Chr1:68715044-68715063 MsG0180003819.01.T01:intron 10.0%
!!! AAAATTTTCAGAAAATAAAT+AGG - Chr1:68713299-68713318 MsG0180003819.01.T01:intron 10.0%
!!! ATTTTTTTATAAACAAGTTT+TGG - Chr1:68712005-68712024 MsG0180003819.01.T01:CDS 10.0%
!! CACAATAATTAATCAATTTA+AGG + Chr1:68713599-68713618 None:intergenic 15.0%
!! CTTAAATTGATTAATTATTG+TGG - Chr1:68713597-68713616 MsG0180003819.01.T01:intron 15.0%
!! TAAATTGATTAATTATTGTG+GGG - Chr1:68713599-68713618 MsG0180003819.01.T01:intron 15.0%
!!! AATCAAATTTCCTTTTTTAA+TGG + Chr1:68714805-68714824 None:intergenic 15.0%
!!! ATACTTATTTTTTTTTAGAC+AGG + Chr1:68715244-68715263 None:intergenic 15.0%
!!! CAAAAATAGTCATTTTAAAT+AGG - Chr1:68715043-68715062 MsG0180003819.01.T01:intron 15.0%
!!! TATTTTACTAAAAATGCTAA+TGG - Chr1:68713891-68713910 MsG0180003819.01.T01:intron 15.0%
!!! TCTTCTTTGTTTATTTTTTA+TGG - Chr1:68713273-68713292 MsG0180003819.01.T01:intron 15.0%
!!! TTTTTAAAAGTACTTTAAGA+TGG - Chr1:68713637-68713656 MsG0180003819.01.T01:intron 15.0%
!! AAAAATAAACAAAGAAGATG+AGG + Chr1:68713271-68713290 None:intergenic 20.0%
!! AAATGGATATTTGTCTAATA+TGG + Chr1:68713945-68713964 None:intergenic 20.0%
!! ATCATTTCTCTAATTGATTA+TGG - Chr1:68715723-68715742 MsG0180003819.01.T01:intron 20.0%
!! ATTGACAAGTATATATGTAA+TGG + Chr1:68714545-68714564 None:intergenic 20.0%
!! GTGAATATATATTGAATGAA+TGG - Chr1:68714710-68714729 MsG0180003819.01.T01:intron 20.0%
!! TAAAAATAAACTTTAGTGAC+AGG + Chr1:68714506-68714525 None:intergenic 20.0%
!! TAATAAATTAGCAACTTACA+AGG + Chr1:68714165-68714184 None:intergenic 20.0%
!! TACATATTCATTCAACAAAT+GGG - Chr1:68712257-68712276 MsG0180003819.01.T01:intron 20.0%
!! TTAGGTATATTAATTGATTG+AGG - Chr1:68715531-68715550 MsG0180003819.01.T01:intron 20.0%
!! TTCAATATATATTCACTAGT+AGG + Chr1:68714706-68714725 None:intergenic 20.0%
!!! AGTCATTTTTAATCTAATAG+TGG - Chr1:68715015-68715034 MsG0180003819.01.T01:intron 20.0%
!!! ATAAAGATAGTTTTTTTCCA+TGG + Chr1:68712848-68712867 None:intergenic 20.0%
!!! ATCCATTTTTATCTTCATTT+GGG - Chr1:68713957-68713976 MsG0180003819.01.T01:intron 20.0%
!!! ATGTACTTGTTAGAAATTAA+AGG + Chr1:68712242-68712261 None:intergenic 20.0%
!!! ATTGTTTGAACTTAATTTGA+AGG - Chr1:68714044-68714063 MsG0180003819.01.T01:intron 20.0%
!!! CCTTTTATTAAAGAATGATA+GGG - Chr1:68714263-68714282 MsG0180003819.01.T01:intron 20.0%
!!! CTTTAAATCTATTTTTTGCA+GGG + Chr1:68715940-68715959 None:intergenic 20.0%
!!! TAAATTTTGTGAAAGATTTC+TGG - Chr1:68712953-68712972 MsG0180003819.01.T01:intron 20.0%
!!! TATCCATTTTTATCTTCATT+TGG - Chr1:68713956-68713975 MsG0180003819.01.T01:intron 20.0%
!!! TCATTATTTTCTCTTGAATA+TGG + Chr1:68712333-68712352 None:intergenic 20.0%
!!! TCCTTTTATTAAAGAATGAT+AGG - Chr1:68714262-68714281 MsG0180003819.01.T01:intron 20.0%
!!! TTAATTTTTTTTCAAGTGCA+AGG + Chr1:68715827-68715846 None:intergenic 20.0%
!!! TTTTCAATAATTCACGAAAT+TGG + Chr1:68715073-68715092 None:intergenic 20.0%
!!! TTTTCCCTAACTAATTATTA+GGG - Chr1:68712133-68712152 MsG0180003819.01.T01:intron 20.0%
!!! TTTTTCCCTAACTAATTATT+AGG - Chr1:68712132-68712151 MsG0180003819.01.T01:intron 20.0%
! AAAATTGAGAATTCAAGTGA+AGG + Chr1:68713994-68714013 None:intergenic 25.0%
! AAGCTAATAACAGATATTGA+AGG + Chr1:68713508-68713527 None:intergenic 25.0%
! AGAGTTATCTAATATGAACT+AGG - Chr1:68713693-68713712 MsG0180003819.01.T01:intron 25.0%
! AGTTAACTAATTGATGTACA+TGG - Chr1:68713135-68713154 MsG0180003819.01.T01:intron 25.0%
! CAAAAAATTGTTACCAGATT+AGG + Chr1:68712366-68712385 None:intergenic 25.0%
! CAAATTAATAGAGTTACAAC+AGG + Chr1:68715368-68715387 None:intergenic 25.0%
! CATACCCTAATAATTAGTTA+GGG + Chr1:68712140-68712159 None:intergenic 25.0%
! CCCTATCATTCTTTAATAAA+AGG + Chr1:68714266-68714285 None:intergenic 25.0%
! CGAAATAAAGGAAAAACTAA+TGG - Chr1:68714459-68714478 MsG0180003819.01.T01:intron 25.0%
! CTAATAGACTAGTAAATTGA+AGG + Chr1:68715118-68715137 None:intergenic 25.0%
! GTACATATTCATTCAACAAA+TGG - Chr1:68712256-68712275 MsG0180003819.01.T01:intron 25.0%
! TAACTATTACTTGAAAGTCT+TGG + Chr1:68714612-68714631 None:intergenic 25.0%
! TATACCTAAATGATTAGAGA+AGG + Chr1:68715520-68715539 None:intergenic 25.0%
! TATTCCTTCTCTAATCATTT+AGG - Chr1:68715513-68715532 MsG0180003819.01.T01:intron 25.0%
! TCACAAAAGTTGTTTAAAAG+TGG - Chr1:68715690-68715709 MsG0180003819.01.T01:intron 25.0%
! TCATACCCTAATAATTAGTT+AGG + Chr1:68712141-68712160 None:intergenic 25.0%
! TTAAACGTTGAATTAACAGA+AGG + Chr1:68715857-68715876 None:intergenic 25.0%
! TTAGTTATGAGAAGAAGAAA+AGG - Chr1:68712665-68712684 MsG0180003819.01.T01:intron 25.0%
!! ATCTTTAAACCTTTTGAATG+AGG - Chr1:68713918-68713937 MsG0180003819.01.T01:intron 25.0%
!! GATTTGAAATGTCTCTAATA+TGG + Chr1:68714676-68714695 None:intergenic 25.0%
!! TATTAATTTGACCAACTAGA+AGG - Chr1:68715378-68715397 MsG0180003819.01.T01:intron 25.0%
!! TGAATTTTGTATATGTGAGA+CGG + Chr1:68714746-68714765 None:intergenic 25.0%
!! TTATCGTTTTCAATTCAGTT+AGG - Chr1:68714641-68714660 MsG0180003819.01.T01:intron 25.0%
!!! ACTCATTTCATTGTTTTTGT+TGG + Chr1:68711968-68711987 None:intergenic 25.0%
!!! AGTTGATTTGCCATTAAAAA+AGG - Chr1:68714792-68714811 MsG0180003819.01.T01:intron 25.0%
!!! ATATCTGTTATTAGCTTTTG+AGG - Chr1:68713511-68713530 MsG0180003819.01.T01:intron 25.0%
!!! ATTGTTTTTGTTGGTATGTA+CGG + Chr1:68711959-68711978 None:intergenic 25.0%
!!! CACTTTTTTTTGTTTGGATT+AGG - Chr1:68715800-68715819 MsG0180003819.01.T01:intron 25.0%
!!! CTAATCTGGTAACAATTTTT+TGG - Chr1:68712364-68712383 MsG0180003819.01.T01:intron 25.0%
!!! GCTTTAAATCTATTTTTTGC+AGG + Chr1:68715941-68715960 None:intergenic 25.0%
!!! GTTTTGGTGAATCAATATTT+TGG - Chr1:68712021-68712040 MsG0180003819.01.T01:CDS 25.0%
!!! TATCTGTTATTAGCTTTTGA+GGG - Chr1:68713512-68713531 MsG0180003819.01.T01:intron 25.0%
!!! TATTTGTTTTCACTCATTCA+CGG - Chr1:68714880-68714899 MsG0180003819.01.T01:intron 25.0%
!!! TCTGGTTAATATTGATTTTG+CGG - Chr1:68716085-68716104 MsG0180003819.01.T01:intron 25.0%
!!! TCTTCTATCATATGTTGTTT+TGG - Chr1:68713376-68713395 MsG0180003819.01.T01:intron 25.0%
!!! TTGGGTTTTGTGATTAATTT+AGG - Chr1:68715296-68715315 MsG0180003819.01.T01:intron 25.0%
!!! TTTTTTACCAAAAAGAGTAG+AGG - Chr1:68713777-68713796 MsG0180003819.01.T01:intron 25.0%
AAATTGTTACCAGATTAGGT+TGG + Chr1:68712362-68712381 None:intergenic 30.0%
AATCACAAAACCCAAAAGTA+GGG + Chr1:68715291-68715310 None:intergenic 30.0%
ACATACACAAATTGATGAAG+AGG + Chr1:68714914-68714933 None:intergenic 30.0%
ATAACTAATACCTCTTGCTT+TGG + Chr1:68712653-68712672 None:intergenic 30.0%
ATAGAGAGAGAAAAATGAAG+AGG - Chr1:68715628-68715647 MsG0180003819.01.T01:CDS 30.0%
CATACACAAATTGATGAAGA+GGG + Chr1:68714913-68714932 None:intergenic 30.0%
CCAATAGAAGAGAGAAAAAA+AGG + Chr1:68715599-68715618 None:intergenic 30.0%
GAAGAGAAATGACGAAATAA+AGG - Chr1:68714447-68714466 MsG0180003819.01.T01:intron 30.0%
GACTGATACATAATAAGTGA+GGG - Chr1:68712390-68712409 MsG0180003819.01.T01:intron 30.0%
GTAACAAAGTTGACAATGTA+CGG - Chr1:68716316-68716335 MsG0180003819.01.T01:CDS 30.0%
GTCTTCTGATTAAGCTTAAA+GGG + Chr1:68714289-68714308 None:intergenic 30.0%
TAATATGGACCTCATTCAAA+AGG + Chr1:68713930-68713949 None:intergenic 30.0%
TAATCACAAAACCCAAAAGT+AGG + Chr1:68715292-68715311 None:intergenic 30.0%
TAGAGAGAGAAAAATGAAGA+GGG - Chr1:68715629-68715648 MsG0180003819.01.T01:CDS 30.0%
TCAAATTGACCAAAGGTAAA+CGG + Chr1:68714367-68714386 None:intergenic 30.0%
TCACTCAAAGGAAATAAAGA+AGG + Chr1:68715448-68715467 None:intergenic 30.0%
TCATCAATCAAATTGACCAA+AGG + Chr1:68714374-68714393 None:intergenic 30.0%
TCTTCTAATGAACTAAGCTA+AGG - Chr1:68713336-68713355 MsG0180003819.01.T01:intron 30.0%
TGAAATGTCTCTAATATGGA+TGG + Chr1:68714672-68714691 None:intergenic 30.0%
TGTCTTCTGATTAAGCTTAA+AGG + Chr1:68714290-68714309 None:intergenic 30.0%
TTCAAACAATTGCTCAAGAT+AGG + Chr1:68714034-68714053 None:intergenic 30.0%
TTCAAGTGAAGGAAATAAGA+TGG + Chr1:68713983-68714002 None:intergenic 30.0%
TTCATAAAACTTAGGGATTG+CGG + Chr1:68715339-68715358 None:intergenic 30.0%
TTGTTAAACAATTCATCGAG+CGG - Chr1:68715213-68715232 MsG0180003819.01.T01:intron 30.0%
! ATCCAAACAAAAAAAAGTGC+AGG + Chr1:68715799-68715818 None:intergenic 30.0%
! CTTAATTTGAAGGTATAGCT+AGG - Chr1:68714054-68714073 MsG0180003819.01.T01:intron 30.0%
! TCCAAACAAAAAAAAGTGCA+GGG + Chr1:68715798-68715817 None:intergenic 30.0%
!! TTGGTGAATCAATATTTTGG+TGG - Chr1:68712024-68712043 MsG0180003819.01.T01:CDS 30.0%
!!! CCTTTTTTTCTCTCTTCTAT+TGG - Chr1:68715596-68715615 MsG0180003819.01.T01:CDS 30.0%
!!! TCTGGTAACAATTTTTTGGT+TGG - Chr1:68712368-68712387 MsG0180003819.01.T01:intron 30.0%
AAAGAACAAGAGATGTTTCC+AGG + Chr1:68712086-68712105 None:intergenic 35.0%
AAAGGCAATTGCAATTGCAT+TGG + Chr1:68712751-68712770 None:intergenic 35.0%
AAGCCAGAAACAAATCCATT+TGG - Chr1:68712580-68712599 MsG0180003819.01.T01:intron 35.0%
AAGTACTAGTAGTAGCTAGT+AGG - Chr1:68712906-68712925 MsG0180003819.01.T01:intron 35.0%
ACTAGAAGGAAATGCACAAT+AGG - Chr1:68715392-68715411 MsG0180003819.01.T01:intron 35.0%
AGCAGTAACTCTTCAATTGA+TGG + Chr1:68714342-68714361 None:intergenic 35.0%
ATGTCCATTAAGACAAGCTT+GGG - Chr1:68712501-68712520 MsG0180003819.01.T01:intron 35.0%
ATTGTGCATTTCCTTCTAGT+TGG + Chr1:68715392-68715411 None:intergenic 35.0%
CAAAAAGTGATGAAACTGCA+GGG + Chr1:68715767-68715786 None:intergenic 35.0%
CATACAAAAGATCGATCTAG+TGG - Chr1:68712281-68712300 MsG0180003819.01.T01:intron 35.0%
CTAATTGATGTACATGGAGA+AGG - Chr1:68713141-68713160 MsG0180003819.01.T01:intron 35.0%
CTAGAAGGAAATGCACAATA+GGG - Chr1:68715393-68715412 MsG0180003819.01.T01:intron 35.0%
GCAGTAACTCTTCAATTGAT+GGG + Chr1:68714341-68714360 None:intergenic 35.0%
GCGTCTCTTCATAAAACTTA+GGG + Chr1:68715346-68715365 None:intergenic 35.0%
GGACTGATACATAATAAGTG+AGG - Chr1:68712389-68712408 MsG0180003819.01.T01:intron 35.0%
GGAGATGAATTAGCTAGAAT+CGG + Chr1:68714581-68714600 None:intergenic 35.0%
GGCCCAAATGAAGATAAAAA+TGG + Chr1:68713962-68713981 None:intergenic 35.0%
GTTCAGTTCGTTAGCTAATA+AGG + Chr1:68713543-68713562 None:intergenic 35.0%
TAGTAGTAGCTAGTAGGTTT+AGG - Chr1:68712912-68712931 MsG0180003819.01.T01:intron 35.0%
TAGTTGAAGTAGCAGAAAAG+TGG - Chr1:68713097-68713116 MsG0180003819.01.T01:intron 35.0%
TGATACATAATAAGTGAGGG+AGG - Chr1:68712393-68712412 MsG0180003819.01.T01:intron 35.0%
TGATTGACTGACATGTTGTA+CGG + Chr1:68714089-68714108 None:intergenic 35.0%
TGTCTCCTTCAAATGTGATA+GGG - Chr1:68714238-68714257 MsG0180003819.01.T01:intron 35.0%
TTCCTTTGAGTGATTCTAGT+GGG - Chr1:68715455-68715474 MsG0180003819.01.T01:intron 35.0%
TTGAAAGTCTTGGTAAACCA+AGG + Chr1:68714602-68714621 None:intergenic 35.0%
TTGTCTCCTTCAAATGTGAT+AGG - Chr1:68714237-68714256 MsG0180003819.01.T01:intron 35.0%
TTTCCTTTGAGTGATTCTAG+TGG - Chr1:68715454-68715473 MsG0180003819.01.T01:intron 35.0%
! ATGGTGATGATGAAGATGAA+AGG + Chr1:68712769-68712788 None:intergenic 35.0%
! TGAACTTTTGTCTTGCCAAA+TGG + Chr1:68712427-68712446 None:intergenic 35.0%
! TTCTAGCTAATTCATCTCCT+TGG - Chr1:68714582-68714601 MsG0180003819.01.T01:intron 35.0%
! TTTTCACTCATTCACGGAAT+TGG - Chr1:68714886-68714905 MsG0180003819.01.T01:intron 35.0%
!! AGTAGCTTTTGTTTCATCAG+TGG + Chr1:68713177-68713196 None:intergenic 35.0%
!! ATTGGGAAACTCTAAAACTG+CGG - Chr1:68716029-68716048 MsG0180003819.01.T01:intron 35.0%
!! GTAGCTTTTGTTTCATCAGT+GGG + Chr1:68713176-68713195 None:intergenic 35.0%
!!! TCCCTGCACTTTTTTTTGTT+TGG - Chr1:68715794-68715813 MsG0180003819.01.T01:intron 35.0%
!!! TTGAGGGAGTTTTAAGTTGT+TGG + Chr1:68713454-68713473 None:intergenic 35.0%
AAAACCCAAAAGTAGGGAAG+GGG + Chr1:68715285-68715304 None:intergenic 40.0%
AAACAGTACGTTGTTGTCTC+CGG + Chr1:68716066-68716085 None:intergenic 40.0%
AAGAGATGTTTCCAGGTTTC+AGG + Chr1:68712079-68712098 None:intergenic 40.0%
ACAAAACCCAAAAGTAGGGA+AGG + Chr1:68715287-68715306 None:intergenic 40.0%
ACTGACATGTTGTACGGAAT+TGG + Chr1:68714083-68714102 None:intergenic 40.0%
AGACAACAACGTACTGTTTC+TGG - Chr1:68716067-68716086 MsG0180003819.01.T01:intron 40.0%
AGAGATTGAATCCTGAAACC+TGG - Chr1:68712065-68712084 MsG0180003819.01.T01:intron 40.0%
AGATCCTCTATGTCTCAAAG+TGG + Chr1:68715494-68715513 None:intergenic 40.0%
ATTGTGGGGTGCATCTAATA+TGG - Chr1:68713613-68713632 MsG0180003819.01.T01:intron 40.0%
CAAAACCCAAAAGTAGGGAA+GGG + Chr1:68715286-68715305 None:intergenic 40.0%
CATGTCCATTAAGACAAGCT+TGG - Chr1:68712500-68712519 MsG0180003819.01.T01:intron 40.0%
CCAAAAAGTGATGAAACTGC+AGG + Chr1:68715768-68715787 None:intergenic 40.0%
CCACGTGGCATGAAATAATT+GGG + Chr1:68715915-68715934 None:intergenic 40.0%
CCAGGTGCAACTCAATAAAA+TGG - Chr1:68712807-68712826 MsG0180003819.01.T01:intron 40.0%
CCATAGAATGGACAAATCGA+GGG + Chr1:68712454-68712473 None:intergenic 40.0%
GATCTTCAATCCAAAGCAAG+AGG - Chr1:68712640-68712659 MsG0180003819.01.T01:intron 40.0%
GGCGTCTCTTCATAAAACTT+AGG + Chr1:68715347-68715366 None:intergenic 40.0%
GGCTATTCTCTCATATTCCA+TGG - Chr1:68712828-68712847 MsG0180003819.01.T01:intron 40.0%
GTCTCCTTCAAATGTGATAG+GGG - Chr1:68714239-68714258 MsG0180003819.01.T01:intron 40.0%
TCCATAGAATGGACAAATCG+AGG + Chr1:68712455-68712474 None:intergenic 40.0%
TCCTTTGAGTGATTCTAGTG+GGG - Chr1:68715456-68715475 MsG0180003819.01.T01:intron 40.0%
TGATTCTGACCAACCTAATC+TGG - Chr1:68712350-68712369 MsG0180003819.01.T01:intron 40.0%
TGCTTTCGGTAACATGTTTG+TGG + Chr1:68716151-68716170 None:intergenic 40.0%
TGTCCATTAAGACAAGCTTG+GGG - Chr1:68712502-68712521 MsG0180003819.01.T01:intron 40.0%
TGTTAGACTTTACCTTCGTG+AGG - Chr1:68712612-68712631 MsG0180003819.01.T01:intron 40.0%
TTGAAGTAGCAGAAAAGTGG+TGG - Chr1:68713100-68713119 MsG0180003819.01.T01:intron 40.0%
TTTCGGTAACATGTTTGTGG+TGG + Chr1:68716148-68716167 None:intergenic 40.0%
! ATGCACTGATAGGTCGTTTA+AGG - Chr1:68712533-68712552 MsG0180003819.01.T01:intron 40.0%
! CAAGCTTGTCTTAATGGACA+TGG + Chr1:68712502-68712521 None:intergenic 40.0%
! GACATGAGACGAGTTTCTTT+TGG - Chr1:68714306-68714325 MsG0180003819.01.T01:intron 40.0%
! GCACCAAATGGATTTGTTTC+TGG + Chr1:68712586-68712605 None:intergenic 40.0%
! TAGCAGACCTCTACTCTTTT+TGG + Chr1:68713787-68713806 None:intergenic 40.0%
! TGCACTGATAGGTCGTTTAA+GGG - Chr1:68712534-68712553 MsG0180003819.01.T01:intron 40.0%
! TGTCTTAATGGACATGGACA+TGG + Chr1:68712496-68712515 None:intergenic 40.0%
!! CAGCTTTTGAGGAAAATGGA+GGG - Chr1:68712557-68712576 MsG0180003819.01.T01:intron 40.0%
!! CCATTTTATTGAGTTGCACC+TGG + Chr1:68712810-68712829 None:intergenic 40.0%
!! CCTGCAGTTTCATCACTTTT+TGG - Chr1:68715765-68715784 MsG0180003819.01.T01:intron 40.0%
!! TAATAAGTGAGGGAGGTACT+TGG - Chr1:68712400-68712419 MsG0180003819.01.T01:intron 40.0%
!! TTTTATTGAGTTGCACCTGG+AGG + Chr1:68712807-68712826 None:intergenic 40.0%
!!! ATTGATTTTGCGGTGCCATT+AGG - Chr1:68716095-68716114 MsG0180003819.01.T01:intron 40.0%
AAGTAGCAGAAAAGTGGTGG+TGG - Chr1:68713103-68713122 MsG0180003819.01.T01:intron 45.0%
AGGACCCCTATCACATTTGA+AGG + Chr1:68714246-68714265 None:intergenic 45.0%
CATGAAATAATTGGGCCACG+TGG + Chr1:68715907-68715926 None:intergenic 45.0%
CCCAATTATTTCATGCCACG+TGG - Chr1:68715912-68715931 MsG0180003819.01.T01:intron 45.0%
CCTTTGAGTGATTCTAGTGG+GGG - Chr1:68715457-68715476 MsG0180003819.01.T01:intron 45.0%
CTACCCCAAGCTTGTCTTAA+TGG + Chr1:68712508-68712527 None:intergenic 45.0%
GCCACGTGGCATGAAATAAT+TGG + Chr1:68715916-68715935 None:intergenic 45.0%
GGGTTTGGATGTCCATAGAA+TGG + Chr1:68712466-68712485 None:intergenic 45.0%
TACTGCTGACCGTTTACCTT+TGG - Chr1:68714355-68714374 MsG0180003819.01.T01:intron 45.0%
! AAAACTAACAGAGTGCCACG+TGG - Chr1:68715889-68715908 MsG0180003819.01.T01:intron 45.0%
! AGCTGTTGTGGTTGTTGTTG+TGG + Chr1:68712700-68712719 None:intergenic 45.0%
! CCCTCGATTTGTCCATTCTA+TGG - Chr1:68712451-68712470 MsG0180003819.01.T01:intron 45.0%
! CTAACAGCTCTAGCACCAAA+TGG + Chr1:68712598-68712617 None:intergenic 45.0%
! GGTAGTCTTGATGCACTGAT+AGG - Chr1:68712523-68712542 MsG0180003819.01.T01:intron 45.0%
! TCGTTTAAGGGCAGCTTTTG+AGG - Chr1:68712546-68712565 MsG0180003819.01.T01:intron 45.0%
! TGTTGTGGTTGTTGTTGTGG+TGG + Chr1:68712697-68712716 None:intergenic 45.0%
!! AGGGCAGCTTTTGAGGAAAA+TGG - Chr1:68712553-68712572 MsG0180003819.01.T01:intron 45.0%
!! ATCACCCCTTCCCTACTTTT+GGG - Chr1:68715278-68715297 MsG0180003819.01.T01:intron 45.0%
!! GCAGCTTTTGAGGAAAATGG+AGG - Chr1:68712556-68712575 MsG0180003819.01.T01:intron 45.0%
!! GTAAGTTTGGCGGCTGTTTT+TGG - Chr1:68716283-68716302 MsG0180003819.01.T01:intron 45.0%
!! GTGACCACTTTGAGACATAG+AGG - Chr1:68715487-68715506 MsG0180003819.01.T01:intron 45.0%
!! AATAAAAAAAAAAAATATTG+AGG + Chr1:68713471-68713490 None:intergenic 5.0%
!! AATTAATTAAGTATAATAAT+TGG - Chr1:68716011-68716030 MsG0180003819.01.T01:intron 5.0%
!! ATAAAAAAAAAAAATATTGA+GGG + Chr1:68713470-68713489 None:intergenic 5.0%
!! ATTAATTAAGTATAATAATT+GGG - Chr1:68716012-68716031 MsG0180003819.01.T01:intron 5.0%
!!! AAAAAAAATAAGTATAATTT+TGG - Chr1:68715249-68715268 MsG0180003819.01.T01:intron 5.0%
!!! AAGTTTTAATAAAATATTTT+TGG - Chr1:68713743-68713762 MsG0180003819.01.T01:intron 5.0%
!!! ATGATTTTTTTATTTATTTA+TGG - Chr1:68713405-68713424 MsG0180003819.01.T01:intron 5.0%
AAAACCAGAAACGCCGTGAC+TGG - Chr1:68711850-68711869 MsG0180003819.01.T01:CDS 50.0%
AATGGACATGGACATGGTGC+TGG + Chr1:68712490-68712509 None:intergenic 50.0%
ACCAAGTATCCACCGAATCC+TGG + Chr1:68716241-68716260 None:intergenic 50.0%
AGGGCACACGATAGATAGCT+AGG - Chr1:68715412-68715431 MsG0180003819.01.T01:intron 50.0%
CATCTAGAGAGGGAAAGAGG+AGG + Chr1:68711909-68711928 None:intergenic 50.0%
CCAAGTATCCACCGAATCCT+GGG + Chr1:68716240-68716259 None:intergenic 50.0%
CCCCCACTAGAATCACTCAA+AGG + Chr1:68715460-68715479 None:intergenic 50.0%
CGAAAGCAGCTTGACCAGAA+CGG - Chr1:68716163-68716182 MsG0180003819.01.T01:intron 50.0%
CTGCATCTAGAGAGGGAAAG+AGG + Chr1:68711912-68711931 None:intergenic 50.0%
CTTTCCCTCTCTAGATGCAG+CGG - Chr1:68711912-68711931 MsG0180003819.01.T01:CDS 50.0%
GAAGATCACGAACCTCACGA+AGG + Chr1:68712627-68712646 None:intergenic 50.0%
GACGGTGATTCTTGAGGTAC+TGG + Chr1:68711887-68711906 None:intergenic 50.0%
GAGGTGGAAGCTGATGTTGA+TGG + Chr1:68712788-68712807 None:intergenic 50.0%
GGGCACACGATAGATAGCTA+GGG - Chr1:68715413-68715432 MsG0180003819.01.T01:intron 50.0%
TAGAGAGGGAAAGAGGAGGA+CGG + Chr1:68711905-68711924 None:intergenic 50.0%
TGCTGAAGCTGAAGCTGTTG+TGG + Chr1:68712712-68712731 None:intergenic 50.0%
! CGTTCTGGTCAAGCTGCTTT+CGG + Chr1:68716165-68716184 None:intergenic 50.0%
! GATCACCCCTTCCCTACTTT+TGG - Chr1:68715277-68715296 MsG0180003819.01.T01:intron 50.0%
! TATTGAGTTGCACCTGGAGG+TGG + Chr1:68712804-68712823 None:intergenic 50.0%
! TCCTGTATCCCACTTGTTGC+CGG + Chr1:68716201-68716220 None:intergenic 50.0%
!! AGGATTCGGTGGATACTTGG+TGG - Chr1:68716240-68716259 MsG0180003819.01.T01:intron 50.0%
!! AGGGAGGTACTTGGACCATT+TGG - Chr1:68712409-68712428 MsG0180003819.01.T01:intron 50.0%
!! GGCCAAAAGTGTTCCAGTCA+CGG + Chr1:68711866-68711885 None:intergenic 50.0%
!!! ATTTTTTGCAGGGTGCCACG+TGG + Chr1:68715930-68715949 None:intergenic 50.0%
!!! GCGGCTGTTTTTGGAGATCT+TGG - Chr1:68716292-68716311 MsG0180003819.01.T01:intron 50.0%
AACTGCGGATGCAGCAATGC+CGG - Chr1:68716044-68716063 MsG0180003819.01.T01:intron 55.0%
AAGCAGCTTGACCAGAACGG+CGG - Chr1:68716166-68716185 MsG0180003819.01.T01:intron 55.0%
AGCACCGCTGCATCTAGAGA+GGG + Chr1:68711919-68711938 None:intergenic 55.0%
ATCCACCGAATCCTGGGTAC+TGG + Chr1:68716234-68716253 None:intergenic 55.0%
CAACATCAGCTTCCACCTCC+AGG - Chr1:68712789-68712808 MsG0180003819.01.T01:intron 55.0%
CACGACCAGTACCCAGGATT+CGG - Chr1:68716226-68716245 MsG0180003819.01.T01:intron 55.0%
CCATTAGGCCAGATTCGGGT+AGG - Chr1:68716110-68716129 MsG0180003819.01.T01:intron 55.0%
CCTACCCGAATCTGGCCTAA+TGG + Chr1:68716113-68716132 None:intergenic 55.0%
GAGGAGGACGGTGATTCTTG+AGG + Chr1:68711893-68711912 None:intergenic 55.0%
GGTTGACGGCGTAAGTTTGG+CGG - Chr1:68716273-68716292 MsG0180003819.01.T01:intron 55.0%
TAGGCCAGATTCGGGTAGGA+GGG - Chr1:68716114-68716133 MsG0180003819.01.T01:intron 55.0%
TCCGGCAACAAGTGGGATAC+AGG - Chr1:68716197-68716216 MsG0180003819.01.T01:intron 55.0%
TGATCCCTCCTACCCGAATC+TGG + Chr1:68716121-68716140 None:intergenic 55.0%
TGCGGTTGACGGCGTAAGTT+TGG - Chr1:68716270-68716289 MsG0180003819.01.T01:intron 55.0%
TGTTCCAGTCACGGCGTTTC+TGG + Chr1:68711857-68711876 None:intergenic 55.0%
TTAGGCCAGATTCGGGTAGG+AGG - Chr1:68716113-68716132 MsG0180003819.01.T01:intron 55.0%
TTTCGCCTCCGGCAACAAGT+GGG - Chr1:68716190-68716209 MsG0180003819.01.T01:intron 55.0%
! ATACTTGGTGGCCGGCTTTG+CGG - Chr1:68716252-68716271 MsG0180003819.01.T01:intron 55.0%
! CGCCGTGACTGGAACACTTT+TGG - Chr1:68711861-68711880 MsG0180003819.01.T01:CDS 55.0%
! GACATGGACATGGTGCTGGA+GGG + Chr1:68712486-68712505 None:intergenic 55.0%
! TGTATCCCACTTGTTGCCGG+AGG + Chr1:68716198-68716217 None:intergenic 55.0%
! TTCGGTGGATACTTGGTGGC+CGG - Chr1:68716244-68716263 MsG0180003819.01.T01:intron 55.0%
!! CCCAGGATTCGGTGGATACT+TGG - Chr1:68716237-68716256 MsG0180003819.01.T01:intron 55.0%
!! CGGTGCCATTAGGCCAGATT+CGG - Chr1:68716105-68716124 MsG0180003819.01.T01:intron 55.0%
!! GGTGCCATTAGGCCAGATTC+GGG - Chr1:68716106-68716125 MsG0180003819.01.T01:intron 55.0%
AGACAGCACGACCAGTACCC+AGG - Chr1:68716220-68716239 MsG0180003819.01.T01:intron 60.0%
AGCAGCTTGACCAGAACGGC+GGG - Chr1:68716167-68716186 MsG0180003819.01.T01:intron 60.0%
GACCAGTACCCAGGATTCGG+TGG - Chr1:68716229-68716248 MsG0180003819.01.T01:intron 60.0%
GTTTCGCCTCCGGCAACAAG+TGG - Chr1:68716189-68716208 MsG0180003819.01.T01:intron 60.0%
TACGCCGTCAACCGCAAAGC+CGG + Chr1:68716266-68716285 None:intergenic 60.0%
! GAGCACCGCTGCATCTAGAG+AGG + Chr1:68711920-68711939 None:intergenic 60.0%
! GGACATGGACATGGTGCTGG+AGG + Chr1:68712487-68712506 None:intergenic 60.0%
! GGACATGGTGCTGGAGGGTT+TGG + Chr1:68712481-68712500 None:intergenic 60.0%
! AGAACGGCGGGTTTCGCCTC+CGG - Chr1:68716179-68716198 MsG0180003819.01.T01:intron 65.0%
! GTGGCCGGCTTTGCGGTTGA+CGG - Chr1:68716259-68716278 MsG0180003819.01.T01:intron 65.0%
GGAGGCGAAACCCGCCGTTC+TGG + Chr1:68716180-68716199 None:intergenic 70.0%
Chromosome Type Strat End Strand Name
Chr1 gene 68711847 68716354 68711847 ID=MsG0180003819.01;Name=MsG0180003819.01
Chr1 mRNA 68711847 68716354 68711847 ID=MsG0180003819.01.T01;Parent=MsG0180003819.01;Name=MsG0180003819.01.T01;_AED=0.73;_eAED=0.80;_QI=0|0|0|0.66|1|1|3|0|129
Chr1 exon 68716315 68716354 68716315 ID=MsG0180003819.01.T01:exon:24331;Parent=MsG0180003819.01.T01
Chr1 exon 68715540 68715656 68715540 ID=MsG0180003819.01.T01:exon:24330;Parent=MsG0180003819.01.T01
Chr1 exon 68711847 68712079 68711847 ID=MsG0180003819.01.T01:exon:24329;Parent=MsG0180003819.01.T01
Chr1 CDS 68716315 68716354 68716315 ID=MsG0180003819.01.T01:cds;Parent=MsG0180003819.01.T01
Chr1 CDS 68715540 68715656 68715540 ID=MsG0180003819.01.T01:cds;Parent=MsG0180003819.01.T01
Chr1 CDS 68711847 68712079 68711847 ID=MsG0180003819.01.T01:cds;Parent=MsG0180003819.01.T01
Gene Sequence

>MsG0180003819.01.T01

ATGAAAACCAGAAACGCCGTGACTGGAACACTTTTGGCCAGTCGTTTAAGGGCAGCTTTTGAGGAAAATGGAGGGAAGCCAGAAACAAATCCATTTGGTGCTAGAGCTGTTAGACTTTACCTTCGTGAGGTTCGTGATCTTCAATCCAAAGCAAGAGATTCGGGTAGGAGGGATCATGCGCCACCACAAACATGTTACCGAAAGCAGCTTGACCAGAACGGCGGGTTTCGCCTCCGGCAACAAGTGGGATACAGGAGACAGCACGACCAGTACCCAGGATTCGGTGGATACTTGGTGGCCGGCTTTGCGGTTGACGGCGTAAGTTTGGCGGCTGTTTTTGGAGATCTTGGAGTAACAAAGTTGACAATGTACGGATCGAAGCATGCATGA
Protein sequence

>MsG0180003819.01.T01

MKTRNAVTGTLLASRLRAAFEENGGKPETNPFGARAVRLYLREVRDLQSKARDSGRRDHAPPQTCYRKQLDQNGGFRLRQQVGYRRQHDQYPGFGGYLVAGFAVDGVSLAAVFGDLGVTKLTMYGSKHA*