Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280007414.01.T01 | APM87895.1 | 96.721 | 61 | 2 | 0 | 1 | 61 | 64 | 124 | 2.99E-35 | 125 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280007414.01.T01 | P46608 | 89.706 | 68 | 7 | 0 | 1 | 68 | 252 | 319 | 6.76E-29 | 108 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280007414.01.T01 | A0A6A3C7A6 | 96.825 | 63 | 2 | 0 | 2 | 64 | 253 | 315 | 6.28e-36 | 134 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048357.01 | MsG0280007414.01 | 0.865280 | 6.620014e-65 | 2.141825e-61 |
| MsG0180000353.01 | MsG0280007414.01 | 0.808037 | 3.770018e-50 | 2.132909e-47 |
| MsG0180000373.01 | MsG0280007414.01 | 0.813121 | 3.004871e-51 | 1.945281e-48 |
| MsG0180000632.01 | MsG0280007414.01 | 0.848097 | 7.614230e-60 | 1.381830e-56 |
| MsG0180001200.01 | MsG0280007414.01 | 0.825293 | 5.073463e-54 | 4.605405e-51 |
| MsG0180001771.01 | MsG0280007414.01 | 0.848262 | 6.856679e-60 | 1.251103e-56 |
| MsG0180002101.01 | MsG0280007414.01 | 0.806911 | 6.537147e-50 | 3.591500e-47 |
| MsG0180003361.01 | MsG0280007414.01 | 0.898282 | 6.178109e-77 | 7.251966e-73 |
| MsG0180003819.01 | MsG0280007414.01 | 0.804520 | 2.077114e-49 | 1.073169e-46 |
| MsG0180003920.01 | MsG0280007414.01 | 0.800444 | 1.437106e-48 | 6.692594e-46 |
| MsG0180004143.01 | MsG0280007414.01 | 0.846830 | 1.697664e-59 | 2.959202e-56 |
| MsG0180004707.01 | MsG0280007414.01 | 0.829774 | 4.263603e-55 | 4.407368e-52 |
| MsG0180005987.01 | MsG0280007414.01 | 0.803049 | 4.197130e-49 | 2.088114e-46 |
| MsG0280007252.01 | MsG0280007414.01 | 0.810695 | 1.014454e-50 | 6.157173e-48 |
| MsG0280007305.01 | MsG0280007414.01 | 0.891518 | 3.692178e-74 | 3.248029e-70 |
| MsG0280007414.01 | MsG0280007458.01 | 0.804355 | 2.247632e-49 | 1.156440e-46 |
| MsG0280007414.01 | MsG0280007547.01 | 0.804957 | 1.683400e-49 | 8.797199e-47 |
| MsG0280007414.01 | MsG0280008362.01 | 0.804730 | 1.877478e-49 | 9.754207e-47 |
| MsG0280007414.01 | MsG0280010489.01 | 0.811304 | 7.485095e-51 | 4.616755e-48 |
| MsG0280007414.01 | MsG0280010551.01 | 0.804195 | 2.427093e-49 | 1.243596e-46 |
| MsG0280007414.01 | MsG0280011014.01 | 0.815048 | 1.128752e-51 | 7.699357e-49 |
| MsG0280007414.01 | MsG0280011124.01 | 0.803063 | 4.169153e-49 | 2.074906e-46 |
| MsG0280007414.01 | MsG0280011258.01 | 0.831563 | 1.555078e-55 | 1.693911e-52 |
| MsG0280007414.01 | MsG0280011282.01 | 0.810098 | 1.364669e-50 | 8.153132e-48 |
| MsG0280007414.01 | MsG0380011496.01 | 0.828060 | 1.108739e-54 | 1.090021e-51 |
| MsG0280007414.01 | MsG0380011501.01 | 0.829053 | 6.382463e-55 | 6.458152e-52 |
| MsG0280007414.01 | MsG0380016465.01 | 0.846543 | 2.033435e-59 | 3.511969e-56 |
| MsG0280007414.01 | MsG0380016610.01 | 0.810465 | 1.137342e-50 | 6.860608e-48 |
| MsG0280007414.01 | MsG0380017823.01 | 0.801704 | 7.942494e-49 | 3.818494e-46 |
| MsG0280007414.01 | MsG0380017869.01 | 0.880702 | 4.413865e-70 | 2.514111e-66 |
| MsG0280007414.01 | MsG0480018909.01 | 0.884827 | 1.374737e-71 | 9.206998e-68 |
| MsG0280007414.01 | MsG0480019279.01 | 0.803888 | 2.812022e-49 | 1.429621e-46 |
| MsG0280007414.01 | MsG0480019922.01 | 0.830668 | 2.579118e-55 | 2.735805e-52 |
| MsG0280007414.01 | MsG0480020495.01 | 0.810770 | 9.769781e-51 | 5.941655e-48 |
| MsG0280007414.01 | MsG0480020765.01 | 0.808702 | 2.719867e-50 | 1.565858e-47 |
| MsG0280007414.01 | MsG0580025740.01 | 0.865837 | 4.420157e-65 | 1.458647e-61 |
| MsG0280007414.01 | MsG0580025821.01 | 0.816351 | 5.782953e-52 | 4.088150e-49 |
| MsG0280007414.01 | MsG0580026610.01 | 0.853047 | 3.093694e-61 | 6.596819e-58 |
| MsG0280007414.01 | MsG0580027650.01 | 0.803597 | 3.231302e-49 | 1.630516e-46 |
| MsG0280007414.01 | MsG0580027925.01 | 0.811428 | 7.034263e-51 | 4.353283e-48 |
| MsG0280007414.01 | MsG0580028436.01 | 0.801603 | 8.331091e-49 | 3.994964e-46 |
| MsG0280007414.01 | MsG0580029405.01 | 0.829123 | 6.139837e-55 | 6.226020e-52 |
| MsG0280007414.01 | MsG0580029417.01 | 0.897831 | 9.595787e-77 | 1.103486e-72 |
| MsG0280007414.01 | MsG0580029418.01 | 0.908557 | 1.501165e-81 | 2.799509e-77 |
| MsG0280007414.01 | MsG0580029441.01 | 0.800116 | 1.676189e-48 | 7.741093e-46 |
| MsG0280007414.01 | MsG0680031342.01 | 0.827283 | 1.704033e-54 | 1.637753e-51 |
| MsG0280007414.01 | MsG0680031428.01 | 0.809507 | 1.828785e-50 | 1.075693e-47 |
| MsG0280007414.01 | MsG0680031707.01 | 0.875910 | 2.122372e-68 | 1.008963e-64 |
| MsG0280007414.01 | MsG0680035658.01 | 0.859441 | 4.127322e-63 | 1.090195e-59 |
| MsG0280007414.01 | MsG0780036147.01 | 0.818163 | 2.262353e-52 | 1.680634e-49 |
| MsG0280007414.01 | MsG0780036148.01 | 0.806528 | 7.873776e-50 | 4.283467e-47 |
| MsG0280007414.01 | MsG0780038424.01 | 0.809146 | 2.185972e-50 | 1.273504e-47 |
| MsG0280007414.01 | MsG0780038688.01 | 0.801695 | 7.973329e-49 | 3.832454e-46 |
| MsG0280007414.01 | MsG0780040432.01 | 0.823134 | 1.631471e-53 | 1.392806e-50 |
| MsG0280007414.01 | MsG0780040846.01 | 0.832546 | 8.886540e-56 | 9.964956e-53 |
| MsG0280007414.01 | MsG0780040966.01 | 0.843615 | 1.256350e-58 | 1.976858e-55 |
| MsG0280007414.01 | MsG0780041205.01 | 0.810068 | 1.385396e-50 | 8.270428e-48 |
| MsG0280007414.01 | MsG0780041535.01 | 0.817877 | 2.624725e-52 | 1.934637e-49 |
| MsG0280007414.01 | MsG0780041676.01 | 0.823760 | 1.164308e-53 | 1.011798e-50 |
| MsG0280007414.01 | MsG0880041858.01 | 0.835747 | 1.401301e-56 | 1.729584e-53 |
| MsG0280007414.01 | MsG0880041860.01 | 0.811257 | 7.663222e-51 | 4.721024e-48 |
| MsG0280007414.01 | MsG0880042442.01 | 0.846365 | 2.273901e-59 | 3.904460e-56 |
| MsG0280007414.01 | MsG0880042734.01 | 0.815097 | 1.100788e-51 | 7.518302e-49 |
| MsG0280007414.01 | MsG0880042961.01 | 0.836424 | 9.434028e-57 | 1.188425e-53 |
| MsG0280007414.01 | MsG0880045585.01 | 0.826583 | 2.504837e-54 | 2.358658e-51 |
| MsG0280007414.01 | MsG0880045586.01 | 0.817734 | 2.827596e-52 | 2.076071e-49 |
| MsG0280007414.01 | MsG0880046580.01 | 0.831320 | 1.784599e-55 | 1.929807e-52 |
| MsG0280007414.01 | MsG0880046636.01 | 0.836247 | 1.046475e-56 | 1.311453e-53 |
| MsG0280007414.01 | MsG0880046942.01 | 0.803058 | 4.178842e-49 | 2.079481e-46 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280007414.01.T01 | MTR_2g024390 | 92.647 | 68 | 5 | 0 | 1 | 68 | 247 | 314 | 7.89e-31 | 110 |
| MsG0280007414.01.T01 | MTR_1g017080 | 73.134 | 67 | 18 | 0 | 2 | 68 | 253 | 319 | 5.52e-21 | 84.3 |
| MsG0280007414.01.T01 | MTR_5g085860 | 84.746 | 59 | 9 | 0 | 5 | 63 | 177 | 235 | 3.39e-19 | 78.6 |
| MsG0280007414.01.T01 | MTR_5g033720 | 60.938 | 64 | 25 | 0 | 5 | 68 | 208 | 271 | 7.37e-16 | 69.7 |
| MsG0280007414.01.T01 | MTR_1g084060 | 62.295 | 61 | 23 | 0 | 8 | 68 | 162 | 222 | 4.52e-15 | 67.0 |
| MsG0280007414.01.T01 | MTR_1g084060 | 62.295 | 61 | 23 | 0 | 8 | 68 | 162 | 222 | 6.23e-15 | 67.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280007414.01.T01 | AT1G62360 | 95.082 | 61 | 3 | 0 | 1 | 61 | 253 | 313 | 1.10e-28 | 105 |
| MsG0280007414.01.T01 | AT4G08150 | 73.134 | 67 | 18 | 0 | 2 | 68 | 271 | 337 | 9.05e-21 | 84.0 |
| MsG0280007414.01.T01 | AT1G23380 | 63.158 | 57 | 21 | 0 | 5 | 61 | 219 | 275 | 8.53e-17 | 72.4 |
| MsG0280007414.01.T01 | AT1G70510 | 63.158 | 57 | 21 | 0 | 5 | 61 | 207 | 263 | 1.50e-16 | 71.6 |
| MsG0280007414.01.T01 | AT1G70510 | 63.158 | 57 | 21 | 0 | 5 | 61 | 203 | 259 | 1.63e-16 | 71.6 |
| MsG0280007414.01.T01 | AT1G23380 | 63.158 | 57 | 21 | 0 | 5 | 61 | 221 | 277 | 2.55e-16 | 71.2 |
Find 16 sgRNAs with CRISPR-Local
Find 18 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGCAGAAGACAGGGAATTAA+AGG | 0.275275 | 2:-15069546 | MsG0280007414.01.T01:CDS |
| AATTGTTGCCTTGCCTCTTT+TGG | 0.282916 | 2:+15069443 | None:intergenic |
| CAAGGCAACAATTGCTTGAA+TGG | 0.428670 | 2:-15069433 | MsG0280007414.01.T01:CDS |
| AATTCCCTGTCTTCTGCTTG+AGG | 0.446914 | 2:+15069551 | None:intergenic |
| AGCAAGAGTTTATGAAGAAA+AGG | 0.455849 | 2:-15069481 | MsG0280007414.01.T01:CDS |
| AGAAAATACAGTGGATACTT+AGG | 0.456169 | 2:-15069512 | MsG0280007414.01.T01:CDS |
| TTTAAAGTGGTGCTTACAGA+TGG | 0.482934 | 2:+15069383 | None:intergenic |
| TAGATCCTCAAGCAGAAGAC+AGG | 0.488640 | 2:-15069556 | MsG0280007414.01.T01:CDS |
| GCAGAAGACAGGGAATTAAA+GGG | 0.524288 | 2:-15069545 | MsG0280007414.01.T01:CDS |
| AAAAGGAAAGCTTCCAAAAG+AGG | 0.530019 | 2:-15069456 | MsG0280007414.01.T01:CDS |
| TTAAAGTGGTGCTTACAGAT+GGG | 0.585949 | 2:+15069384 | None:intergenic |
| GGCAACAATTGCTTGAATGG+TGG | 0.608396 | 2:-15069430 | MsG0280007414.01.T01:CDS |
| GAAAGCTTCCAAAAGAGGCA+AGG | 0.624760 | 2:-15069451 | MsG0280007414.01.T01:CDS |
| GTGGTGCTTACAGATGGGTA+AGG | 0.637343 | 2:+15069389 | None:intergenic |
| AGATCCTCAAGCAGAAGACA+GGG | 0.640900 | 2:-15069555 | MsG0280007414.01.T01:CDS |
| GGTGGAGCAGACATTACAAG+TGG | 0.680780 | 2:-15069412 | MsG0280007414.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTTATGAAGAAAAGGAAAAA+AGG | - | Chr2:15069463-15069482 | MsG0280007414.01.T01:CDS | 20.0% |
| AGAAAATACAGTGGATACTT+AGG | - | Chr2:15069424-15069443 | MsG0280007414.01.T01:CDS | 30.0% | |
| AGCAAGAGTTTATGAAGAAA+AGG | - | Chr2:15069455-15069474 | MsG0280007414.01.T01:CDS | 30.0% | |
| AAAAGGAAAGCTTCCAAAAG+AGG | - | Chr2:15069480-15069499 | MsG0280007414.01.T01:CDS | 35.0% | |
| TTAAAGTGGTGCTTACAGAT+GGG | + | Chr2:15069555-15069574 | None:intergenic | 35.0% | |
| ! | TTTAAAGTGGTGCTTACAGA+TGG | + | Chr2:15069556-15069575 | None:intergenic | 35.0% |
| !! | CAGCTTTTGAGAAAATACAG+TGG | - | Chr2:15069415-15069434 | MsG0280007414.01.T01:CDS | 35.0% |
| AGCAGAAGACAGGGAATTAA+AGG | - | Chr2:15069390-15069409 | MsG0280007414.01.T01:CDS | 40.0% | |
| CAAGGCAACAATTGCTTGAA+TGG | - | Chr2:15069503-15069522 | MsG0280007414.01.T01:CDS | 40.0% | |
| GCAGAAGACAGGGAATTAAA+GGG | - | Chr2:15069391-15069410 | MsG0280007414.01.T01:CDS | 40.0% | |
| ! | AATTGTTGCCTTGCCTCTTT+TGG | + | Chr2:15069496-15069515 | None:intergenic | 40.0% |
| AATTCCCTGTCTTCTGCTTG+AGG | + | Chr2:15069388-15069407 | None:intergenic | 45.0% | |
| AGATCCTCAAGCAGAAGACA+GGG | - | Chr2:15069381-15069400 | MsG0280007414.01.T01:CDS | 45.0% | |
| GAAAGCTTCCAAAAGAGGCA+AGG | - | Chr2:15069485-15069504 | MsG0280007414.01.T01:CDS | 45.0% | |
| GGCAACAATTGCTTGAATGG+TGG | - | Chr2:15069506-15069525 | MsG0280007414.01.T01:CDS | 45.0% | |
| TAGATCCTCAAGCAGAAGAC+AGG | - | Chr2:15069380-15069399 | MsG0280007414.01.T01:CDS | 45.0% | |
| GGTGGAGCAGACATTACAAG+TGG | - | Chr2:15069524-15069543 | MsG0280007414.01.T01:CDS | 50.0% | |
| GTGGTGCTTACAGATGGGTA+AGG | + | Chr2:15069550-15069569 | None:intergenic | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr2 | gene | 15069376 | 15069582 | 15069376 | ID=MsG0280007414.01;Name=MsG0280007414.01 |
| Chr2 | mRNA | 15069376 | 15069582 | 15069376 | ID=MsG0280007414.01.T01;Parent=MsG0280007414.01;Name=MsG0280007414.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|0|1|0|68 |
| Chr2 | exon | 15069376 | 15069582 | 15069376 | ID=MsG0280007414.01.T01:exon:40496;Parent=MsG0280007414.01.T01 |
| Chr2 | CDS | 15069376 | 15069582 | 15069376 | ID=MsG0280007414.01.T01:cds;Parent=MsG0280007414.01.T01 |
| Gene Sequence |
| Protein sequence |
