AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0180004228.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180004228.01.T01 MTR_1g077570 65.625 192 11 2 1 169 1 160 9.30e-70 209
MsG0180004228.01.T02 MTR_1g077570 65.625 192 11 2 1 169 1 160 9.30e-70 209
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180004228.01.T01 AT3G05070 44.444 171 58 3 1 169 1 136 1.18e-32 114
MsG0180004228.01.T02 AT3G05070 44.444 171 58 3 1 169 1 136 1.18e-32 114

Find 49 sgRNAs with CRISPR-Local

Find 163 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGTTCTTCGTCTTGATTAT+TGG 0.250780 1:-75131904 None:intergenic
TGTTCTTCGTCTTGATTATT+GGG 0.276709 1:-75131903 None:intergenic
ACTTGTTTGATTTGAAGTTC+TGG 0.291491 1:+75134640 MsG0180004228.01.T01:three_prime_UTR
TGAAACTCCTTATCGTATTC+AGG 0.296236 1:+75134400 MsG0180004228.01.T01:three_prime_UTR
TTCGTCTTGATTATTGGGTT+CGG 0.316915 1:-75131898 None:intergenic
ACAAGTTACAACCTAATCTT+TGG 0.319987 1:-75134345 None:intergenic
AAAGAAGATTAAATTGAAAA+TGG 0.322479 1:-75134794 None:intergenic
CAGCTTAGCGCATGTAAAAT+TGG 0.330220 1:+75134423 MsG0180004228.01.T01:three_prime_UTR
TTGATTATTGGGTTCGGGTT+CGG 0.333205 1:-75131892 None:intergenic
TCGTCTTGATTATTGGGTTC+GGG 0.338669 1:-75131897 None:intergenic
GAGTTTGAACAAAAGAGGTT+AGG 0.373720 1:+75131713 None:intergenic
GGGTTCGGGTTCGGAGGAAT+TGG 0.388909 1:-75131883 None:intergenic
TTGCTCAATTGATTCGTCTT+GGG 0.400292 1:-75131805 None:intergenic
CTTGCTCAATTGATTCGTCT+TGG 0.402837 1:-75131806 None:intergenic
GAAGAAGTGGGTGGTGGTTG+TGG 0.407660 1:-75131927 None:intergenic
GCTAGAATTTCCTCTAAAAC+AGG 0.416554 1:-75134539 None:intergenic
TGATGATCAACAAGAAGAAA+AGG 0.429236 1:+75131959 MsG0180004228.01.T01:CDS
GCTCTATCCGATTGAATTCA+AGG 0.439363 1:+75134738 MsG0180004228.01.T01:three_prime_UTR
TTGTTGATCATCATCGTCTT+CGG 0.439513 1:-75131949 None:intergenic
AGTTTGAACAAAAGAGGTTA+GGG 0.443788 1:+75131714 None:intergenic
CGATTGAGTTTGAACAAAAG+AGG 0.460595 1:+75131708 None:intergenic
ACAAGTAATATCTTTACATA+TGG 0.461634 1:-75134623 None:intergenic
ATTTATTGAGCAATCAATCA+TGG 0.477559 1:+75134823 MsG0180004228.01.T02:three_prime_UTR
AGCGCATGTAAAATTGGAAT+CGG 0.482329 1:+75134429 MsG0180004228.01.T01:three_prime_UTR
TCAAGACCCTTGAATTCAAT+CGG 0.492932 1:-75134745 None:intergenic
TACAAACGGCGCCAAAGATT+AGG 0.500555 1:+75134334 MsG0180004228.01.T01:CDS
CAGAAAGACATCAACGCCTT+GGG 0.504121 1:-75134707 None:intergenic
AGCTAGGAGTCTTTCACGGC+GGG 0.515574 1:-75131838 None:intergenic
GGATCTAACACACCCGTGCT+TGG 0.520176 1:-75134518 None:intergenic
TCTTTCACGGCGGGAAGCGA+CGG 0.522634 1:-75131829 None:intergenic
GATGATCAACAAGAAGAAAA+GGG 0.526460 1:+75131960 MsG0180004228.01.T01:CDS
ACAGAAAGACATCAACGCCT+TGG 0.529536 1:-75134708 None:intergenic
GTTGAGCGGCGCGAAGAGCT+AGG 0.534647 1:-75131854 None:intergenic
TAAGCTGCCTGAATACGATA+AGG 0.540629 1:-75134407 None:intergenic
ATCATCGTCTTCGGAAGAAG+TGG 0.558826 1:-75131940 None:intergenic
TCTTGCAAAACTGCAGCAAA+TGG 0.563759 1:+75134462 MsG0180004228.01.T01:intron
CCGTGCTTGGCAAATAAAAG+AGG 0.571190 1:-75134505 None:intergenic
AAGATTAAATTGAAAATGGT+AGG 0.572470 1:-75134790 None:intergenic
AGCTGAGGAGATGATACAAA+CGG 0.575262 1:+75134320 MsG0180004228.01.T01:CDS
ATTATTGGGTTCGGGTTCGG+AGG 0.580289 1:-75131889 None:intergenic
GAAGAGCTAGGAGTCTTTCA+CGG 0.584484 1:-75131842 None:intergenic
TCTTCGGAAGAAGTGGGTGG+TGG 0.588852 1:-75131933 None:intergenic
CTCTATCCGATTGAATTCAA+GGG 0.589025 1:+75134739 MsG0180004228.01.T01:three_prime_UTR
GAAGCAAAATCAGTTAGCTG+AGG 0.616037 1:+75134305 MsG0180004228.01.T01:CDS
ACGCATGTTTAAGCTTCCCA+AGG 0.626440 1:+75134691 MsG0180004228.01.T01:three_prime_UTR
GGAATTGGATAATTGTTGAG+CGG 0.629730 1:-75131868 None:intergenic
TCATCGTCTTCGGAAGAAGT+GGG 0.638207 1:-75131939 None:intergenic
GAGCTAGGAGTCTTTCACGG+CGG 0.702871 1:-75131839 None:intergenic
TCGTCTTCGGAAGAAGTGGG+TGG 0.708061 1:-75131936 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CTATAATAAAAGATAAAATA+TGG - Chr1:75132781-75132800 None:intergenic 10.0%
!! AAAGAAGATTAAATTGAAAA+TGG - Chr1:75134797-75134816 None:intergenic 15.0%
!! CTAATAGTACTAAAAAATTA+CGG - Chr1:75134150-75134169 None:intergenic 15.0%
!! TTGTCATATTTATAATTTGA+TGG + Chr1:75133698-75133717 MsG0180004228.01.T01:intron 15.0%
!!! TTTCTTATTTTCTTACTATT+TGG + Chr1:75133251-75133270 MsG0180004228.01.T01:intron 15.0%
!!! TTTTTTAAATTTTTCAACAG+TGG + Chr1:75134275-75134294 MsG0180004228.01.T01:intron 15.0%
!!! TTTTTTGAATTGAATTGAAT+TGG + Chr1:75132028-75132047 MsG0180004228.01.T01:intron 15.0%
!! AAATACTCATAAAATTGCTT+GGG - Chr1:75132983-75133002 None:intergenic 20.0%
!! AAGATTAAATTGAAAATGGT+AGG - Chr1:75134793-75134812 None:intergenic 20.0%
!! ACAAGTAATATCTTTACATA+TGG - Chr1:75134626-75134645 None:intergenic 20.0%
!! AGAAACATTATTGTAAATGT+AGG - Chr1:75133236-75133255 None:intergenic 20.0%
!! ATAAGCTATAAGATCAAAAA+AGG + Chr1:75132714-75132733 MsG0180004228.01.T01:intron 20.0%
!! CCTAATAAAAAGTAGTTAAA+AGG - Chr1:75133068-75133087 None:intergenic 20.0%
!! TAAATACTCATAAAATTGCT+TGG - Chr1:75132984-75133003 None:intergenic 20.0%
!! TAATTGCTTATGTATATTTC+TGG + Chr1:75132277-75132296 MsG0180004228.01.T01:intron 20.0%
!! TGACTAATGAATTAGTATTA+GGG + Chr1:75133877-75133896 MsG0180004228.01.T01:intron 20.0%
!! TTGAATTTGAGAAAAAAGAT+GGG + Chr1:75131780-75131799 MsG0180004228.01.T01:exon 20.0%
!! TTTGCTTAACTAATAATATC+TGG + Chr1:75133821-75133840 MsG0180004228.01.T01:intron 20.0%
!!! AATTGTTTTGTATAAGCTAT+AGG - Chr1:75132755-75132774 None:intergenic 20.0%
!!! ATTGTTTTGTATAAGCTATA+GGG - Chr1:75132754-75132773 None:intergenic 20.0%
!!! CCTTTTAACTACTTTTTATT+AGG + Chr1:75133065-75133084 MsG0180004228.01.T01:intron 20.0%
!!! CGTAATTTTTTAGTACTATT+AGG + Chr1:75134148-75134167 MsG0180004228.01.T01:intron 20.0%
!!! GGTGTTTTTATAAGTTATTA+TGG + Chr1:75132894-75132913 MsG0180004228.01.T01:intron 20.0%
!!! TTTTTCAATTAAGAATCCAT+GGG + Chr1:75132559-75132578 MsG0180004228.01.T01:intron 20.0%
!!! TTTTTTCAATTAAGAATCCA+TGG + Chr1:75132558-75132577 MsG0180004228.01.T01:intron 20.0%
! AAAAGTAGTTAAAAGGTAAG+AGG - Chr1:75133061-75133080 None:intergenic 25.0%
! AAACAGAGTTAGAACAAAAT+TGG + Chr1:75133727-75133746 MsG0180004228.01.T01:intron 25.0%
! AGCATTTCAAAAGCAAAAAA+GGG + Chr1:75134559-75134578 MsG0180004228.01.T01:three_prime_UTR 25.0%
! ATATTCAAGAGAAAGAAAGT+AGG + Chr1:75133339-75133358 MsG0180004228.01.T01:intron 25.0%
! ATTAAGCTGTTTATACAAAC+AGG + Chr1:75132840-75132859 MsG0180004228.01.T01:intron 25.0%
! ATTTATTGAGCAATCAATCA+TGG + Chr1:75134823-75134842 MsG0180004228.01.T02:three_prime_UTR 25.0%
! ATTTGACTAAAAAGACAGTT+TGG - Chr1:75132681-75132700 None:intergenic 25.0%
! CTGACTAATGAATTAGTATT+AGG + Chr1:75133876-75133895 MsG0180004228.01.T01:intron 25.0%
! GAAATTGGCAATAATACTTA+GGG - Chr1:75134057-75134076 None:intergenic 25.0%
! GTTGAATTTGAGAAAAAAGA+TGG + Chr1:75131779-75131798 MsG0180004228.01.T01:exon 25.0%
! TAACTTATAAAAACACCTTC+CGG - Chr1:75132891-75132910 None:intergenic 25.0%
! TACTTAAGTTAATCGAAACA+AGG - Chr1:75132241-75132260 None:intergenic 25.0%
! TAGCATTTCAAAAGCAAAAA+AGG + Chr1:75134558-75134577 MsG0180004228.01.T01:three_prime_UTR 25.0%
! TAGCTTATGGAAATAGATTA+TGG + Chr1:75132372-75132391 MsG0180004228.01.T01:CDS 25.0%
! TATGGATAGTCTATAGAAAT+AGG + Chr1:75132912-75132931 MsG0180004228.01.T01:intron 25.0%
! TATTCAAGAGAAAGAAAGTA+GGG + Chr1:75133340-75133359 MsG0180004228.01.T01:intron 25.0%
! TGAAATTGGCAATAATACTT+AGG - Chr1:75134058-75134077 None:intergenic 25.0%
! TGTAATCATAGAATCAGAAA+TGG - Chr1:75133514-75133533 None:intergenic 25.0%
!! CTTATCATTAATTTTACCCA+TGG - Chr1:75132578-75132597 None:intergenic 25.0%
!! TGTAGGTTTGGTAAAATTAA+CGG + Chr1:75132478-75132497 MsG0180004228.01.T01:intron 25.0%
!! TTTGACACATTTTCAATGAA+TGG + Chr1:75133283-75133302 MsG0180004228.01.T01:intron 25.0%
!! TTTTTGCAATCAGTATTTCA+TGG - Chr1:75133543-75133562 None:intergenic 25.0%
!!! AAATAGATTATGGCTTTGTT+TGG + Chr1:75132382-75132401 MsG0180004228.01.T01:CDS 25.0%
!!! TCAAATTGTTTTATACAAGC+CGG + Chr1:75132869-75132888 MsG0180004228.01.T01:intron 25.0%
ACAAGTTACAACCTAATCTT+TGG - Chr1:75134348-75134367 None:intergenic 30.0%
AGAAACAGGATCTTCAAATT+TGG - Chr1:75132167-75132186 None:intergenic 30.0%
AGAACTTAAGCTGTAATGAA+CGG + Chr1:75133427-75133446 MsG0180004228.01.T01:intron 30.0%
AGTACATACTCAGAAATCTA+TGG - Chr1:75133642-75133661 None:intergenic 30.0%
CAAGACAAAATTTCTTCTGT+AGG + Chr1:75134017-75134036 MsG0180004228.01.T01:intron 30.0%
GAAACAGGATCTTCAAATTT+GGG - Chr1:75132166-75132185 None:intergenic 30.0%
GATGATCAACAAGAAGAAAA+GGG + Chr1:75131960-75131979 MsG0180004228.01.T01:CDS 30.0%
GTACATACTCAGAAATCTAT+GGG - Chr1:75133641-75133660 None:intergenic 30.0%
GTTGATTGAATTTGTAGGTT+TGG + Chr1:75132466-75132485 MsG0180004228.01.T01:intron 30.0%
TATACAAGCAGTTAATGACA+TGG - Chr1:75132954-75132973 None:intergenic 30.0%
TGATGATCAACAAGAAGAAA+AGG + Chr1:75131959-75131978 MsG0180004228.01.T01:CDS 30.0%
TGTTCTTCGTCTTGATTATT+GGG - Chr1:75131906-75131925 None:intergenic 30.0%
TTGTTCTTCGTCTTGATTAT+TGG - Chr1:75131907-75131926 None:intergenic 30.0%
TTTGTTCTAACTCTGTTTCA+TGG - Chr1:75133725-75133744 None:intergenic 30.0%
! ATTTTCAATGAATGGTGAGT+TGG + Chr1:75133291-75133310 MsG0180004228.01.T01:intron 30.0%
! GAACTTGTGAGATTTGAAAT+TGG - Chr1:75134072-75134091 None:intergenic 30.0%
! TTGAGTGATTTGAAATCTGT+CGG + Chr1:75133584-75133603 MsG0180004228.01.T01:intron 30.0%
! TTGATTTTGTTCCATGAATG+TGG + Chr1:75134230-75134249 MsG0180004228.01.T01:intron 30.0%
! TTTTCAATGAATGGTGAGTT+GGG + Chr1:75133292-75133311 MsG0180004228.01.T01:intron 30.0%
!! ACTTGTTTGATTTGAAGTTC+TGG + Chr1:75134640-75134659 MsG0180004228.01.T01:three_prime_UTR 30.0%
!! TTTTTGTTGTGTAGTGAAGA+GGG + Chr1:75132056-75132075 MsG0180004228.01.T01:intron 30.0%
!!! TTTTTTGTTGTGTAGTGAAG+AGG + Chr1:75132055-75132074 MsG0180004228.01.T01:intron 30.0%
AATCCTTAACAAGTGCTCTA+AGG - Chr1:75133919-75133938 None:intergenic 35.0%
AATGAACGGATCAAAACTGT+TGG + Chr1:75133441-75133460 MsG0180004228.01.T01:intron 35.0%
AGAGTTGAACTAACCTCTTT+AGG - Chr1:75132210-75132229 None:intergenic 35.0%
AGCGCATGTAAAATTGGAAT+CGG + Chr1:75134429-75134448 MsG0180004228.01.T01:three_prime_UTR 35.0%
CAAAACTGTTGGATGTGATT+AGG + Chr1:75133452-75133471 MsG0180004228.01.T01:intron 35.0%
CTAGATAGAATAACTTCACC+TGG + Chr1:75133390-75133409 MsG0180004228.01.T01:intron 35.0%
CTATGAAAAACTATAGCCAG+CGG - Chr1:75134103-75134122 None:intergenic 35.0%
CTCTATCCGATTGAATTCAA+GGG + Chr1:75134739-75134758 MsG0180004228.01.T01:three_prime_UTR 35.0%
GGAATTGGATAATTGTTGAG+CGG - Chr1:75131871-75131890 None:intergenic 35.0%
TATAAGCTATAGGGTCTGTT+TGG - Chr1:75132745-75132764 None:intergenic 35.0%
TCAAGACCCTTGAATTCAAT+CGG - Chr1:75134748-75134767 None:intergenic 35.0%
TGAAACTCCTTATCGTATTC+AGG + Chr1:75134400-75134419 MsG0180004228.01.T01:three_prime_UTR 35.0%
TTCGTCTTGATTATTGGGTT+CGG - Chr1:75131901-75131920 None:intergenic 35.0%
TTGGGAGCAATATTGAGAAA+CGG - Chr1:75133093-75133112 None:intergenic 35.0%
TTGTTGATCATCATCGTCTT+CGG - Chr1:75131952-75131971 None:intergenic 35.0%
! GGACTTTGGAGACATTATAA+TGG + Chr1:75134251-75134270 MsG0180004228.01.T01:intron 35.0%
! GGCTAGTTGATTGAATTTGT+AGG + Chr1:75132461-75132480 MsG0180004228.01.T01:intron 35.0%
! TATCCTTAGAGCACTTGTTA+AGG + Chr1:75133913-75133932 MsG0180004228.01.T01:intron 35.0%
!! ACAAGTTCTTTTATTTCCGC+TGG + Chr1:75134084-75134103 MsG0180004228.01.T01:intron 35.0%
!! ATTGTTTTATACAAGCCGGA+AGG + Chr1:75132873-75132892 MsG0180004228.01.T01:intron 35.0%
!! GCTAGAATTTCCTCTAAAAC+AGG - Chr1:75134542-75134561 None:intergenic 35.0%
!! TGTGTTAGATCCTGTTTTAG+AGG + Chr1:75134529-75134548 MsG0180004228.01.T01:three_prime_UTR 35.0%
!! TTGCTCAATTGATTCGTCTT+GGG - Chr1:75131808-75131827 None:intergenic 35.0%
!!! GCAAAAACACTGATTTTCTC+TGG + Chr1:75133556-75133575 MsG0180004228.01.T01:intron 35.0%
AAGCTCTCGAAGATAGCTTA+TGG + Chr1:75132359-75132378 MsG0180004228.01.T01:CDS 40.0%
AAGGCTCTCTATCAACTCAT+GGG + Chr1:75133180-75133199 MsG0180004228.01.T01:CDS 40.0%
ACCTGAGGAAAAACCTAAAG+AGG + Chr1:75132194-75132213 MsG0180004228.01.T01:CDS 40.0%
AGCTGAGGAGATGATACAAA+CGG + Chr1:75134320-75134339 MsG0180004228.01.T01:CDS 40.0%
ATCAGCTCATGAGAAAGTGA+TGG + Chr1:75132652-75132671 MsG0180004228.01.T01:intron 40.0%
ATGACAGTGACAATGACTTG+AGG - Chr1:75133668-75133687 None:intergenic 40.0%
ATTCAGAGTAAGGTCGTGTT+TGG - Chr1:75133036-75133055 None:intergenic 40.0%
CAGCTTAGCGCATGTAAAAT+TGG + Chr1:75134423-75134442 MsG0180004228.01.T01:three_prime_UTR 40.0%
CATGATAAGCATCTTCAGGA+AGG + Chr1:75132119-75132138 MsG0180004228.01.T01:intron 40.0%
CTCATAAAATTGCTTGGGAG+AGG - Chr1:75132978-75132997 None:intergenic 40.0%
GAAGCAAAATCAGTTAGCTG+AGG + Chr1:75134305-75134324 MsG0180004228.01.T01:CDS 40.0%
GCTCTATCCGATTGAATTCA+AGG + Chr1:75134738-75134757 MsG0180004228.01.T01:three_prime_UTR 40.0%
GTAAGAGGCAATTCAGAGTA+AGG - Chr1:75133046-75133065 None:intergenic 40.0%
TAAGCTGCCTGAATACGATA+AGG - Chr1:75134410-75134429 None:intergenic 40.0%
TCAAATTTGGGAAGAACAGC+AGG - Chr1:75132154-75132173 None:intergenic 40.0%
TCAAGAGAAAGAAAGTAGGG+AGG + Chr1:75133343-75133362 MsG0180004228.01.T01:intron 40.0%
TCCTCATGATAAGCATCTTC+AGG + Chr1:75132115-75132134 MsG0180004228.01.T01:intron 40.0%
TCCTGAAGATGCTTATCATG+AGG - Chr1:75132119-75132138 None:intergenic 40.0%
TCGTCTTGATTATTGGGTTC+GGG - Chr1:75131900-75131919 None:intergenic 40.0%
TCTTGCAAAACTGCAGCAAA+TGG + Chr1:75134462-75134481 MsG0180004228.01.T01:intron 40.0%
TGAAAGAACCTGCAAAGACA+TGG - Chr1:75134126-75134145 None:intergenic 40.0%
TGACAGTGACAATGACTTGA+GGG - Chr1:75133667-75133686 None:intergenic 40.0%
TGTTCCATGAATGTGGACTT+TGG + Chr1:75134237-75134256 MsG0180004228.01.T01:intron 40.0%
TTCTAAACTACAGCGAATCC+AGG - Chr1:75133411-75133430 None:intergenic 40.0%
TTTCATAGCCATGTCTTTGC+AGG + Chr1:75134115-75134134 MsG0180004228.01.T01:intron 40.0%
! ATAAGCAGCTGTGACTGTTT+TGG + Chr1:75132301-75132320 MsG0180004228.01.T01:intron 40.0%
! ATGAATGGTGAGTTGGGAAA+TGG + Chr1:75133298-75133317 MsG0180004228.01.T01:intron 40.0%
! CAAGGGTCTTGAGTATTTTC+TGG + Chr1:75134756-75134775 MsG0180004228.01.T01:three_prime_UTR 40.0%
! GTTGATAAGCTAGCTTACAG+TGG + Chr1:75132428-75132447 MsG0180004228.01.T01:intron 40.0%
! TGAATGGTGAGTTGGGAAAT+GGG + Chr1:75133299-75133318 MsG0180004228.01.T01:intron 40.0%
! TTGATTATTGGGTTCGGGTT+CGG - Chr1:75131895-75131914 None:intergenic 40.0%
!! ACCTCTTTAGGTTTTTCCTC+AGG - Chr1:75132198-75132217 None:intergenic 40.0%
!! CCTCTTTTATTTGCCAAGCA+CGG + Chr1:75134505-75134524 MsG0180004228.01.T01:three_prime_UTR 40.0%
!! CTCTTTTATTTGCCAAGCAC+GGG + Chr1:75134506-75134525 MsG0180004228.01.T01:three_prime_UTR 40.0%
!! CTTGCTCAATTGATTCGTCT+TGG - Chr1:75131809-75131828 None:intergenic 40.0%
!! TTTAGGTTTTTCCTCAGGTC+AGG - Chr1:75132193-75132212 None:intergenic 40.0%
ACAGAAAGACATCAACGCCT+TGG - Chr1:75134711-75134730 None:intergenic 45.0%
ACGCATGTTTAAGCTTCCCA+AGG + Chr1:75134691-75134710 MsG0180004228.01.T01:three_prime_UTR 45.0%
ATCATCGTCTTCGGAAGAAG+TGG - Chr1:75131943-75131962 None:intergenic 45.0%
CAGAAAGACATCAACGCCTT+GGG - Chr1:75134710-75134729 None:intergenic 45.0%
CCGTGCTTGGCAAATAAAAG+AGG - Chr1:75134508-75134527 None:intergenic 45.0%
GAAGAGCTAGGAGTCTTTCA+CGG - Chr1:75131845-75131864 None:intergenic 45.0%
GAAGGCTCTCTATCAACTCA+TGG + Chr1:75133179-75133198 MsG0180004228.01.T01:CDS 45.0%
GCTTACAGTGGATAGCGAAT+TGG + Chr1:75132440-75132459 MsG0180004228.01.T01:intron 45.0%
GTCTCCAAAGTCCACATTCA+TGG - Chr1:75134244-75134263 None:intergenic 45.0%
TACAAACGGCGCCAAAGATT+AGG + Chr1:75134334-75134353 MsG0180004228.01.T01:CDS 45.0%
TCATCGTCTTCGGAAGAAGT+GGG - Chr1:75131942-75131961 None:intergenic 45.0%
AGGTCCCAGTTGGGTTTCTT+GGG - Chr1:75133111-75133130 None:intergenic 50.0%
CTCAGGTCAGGAACAGAAAC+AGG - Chr1:75132181-75132200 None:intergenic 50.0%
GCTTGAGAAGCGAACTCAGA+AGG + Chr1:75133161-75133180 MsG0180004228.01.T01:CDS 50.0%
GTTGTGTAGTGAAGAGGGAG+AGG + Chr1:75132061-75132080 MsG0180004228.01.T01:intron 50.0%
TCTTCTGCACATCTCTTCGC+AGG - Chr1:75133131-75133150 None:intergenic 50.0%
TGCTCCCAAGAAACCCAACT+GGG + Chr1:75133104-75133123 MsG0180004228.01.T01:CDS 50.0%
TTGCTCCCAAGAAACCCAAC+TGG + Chr1:75133103-75133122 MsG0180004228.01.T01:CDS 50.0%
! ATTATTGGGTTCGGGTTCGG+AGG - Chr1:75131892-75131911 None:intergenic 50.0%
!! CTGTTTCTGTTCCTGACCTG+AGG + Chr1:75132179-75132198 MsG0180004228.01.T01:CDS 50.0%
AGCTAGGAGTCTTTCACGGC+GGG - Chr1:75131841-75131860 None:intergenic 55.0%
ATCTCTTCGCAGGTCCCAGT+TGG - Chr1:75133121-75133140 None:intergenic 55.0%
CAGGTCCCAGTTGGGTTTCT+TGG - Chr1:75133112-75133131 None:intergenic 55.0%
GAGCTAGGAGTCTTTCACGG+CGG - Chr1:75131842-75131861 None:intergenic 55.0%
GGATCTAACACACCCGTGCT+TGG - Chr1:75134521-75134540 None:intergenic 55.0%
TCGTCTTCGGAAGAAGTGGG+TGG - Chr1:75131939-75131958 None:intergenic 55.0%
TCTCTTCGCAGGTCCCAGTT+GGG - Chr1:75133120-75133139 None:intergenic 55.0%
TCTTCGGAAGAAGTGGGTGG+TGG - Chr1:75131936-75131955 None:intergenic 55.0%
! GAAGAAGTGGGTGGTGGTTG+TGG - Chr1:75131930-75131949 None:intergenic 55.0%
TCTTTCACGGCGGGAAGCGA+CGG - Chr1:75131832-75131851 None:intergenic 60.0%
! GGGTTCGGGTTCGGAGGAAT+TGG - Chr1:75131886-75131905 None:intergenic 60.0%
GTTGAGCGGCGCGAAGAGCT+AGG - Chr1:75131857-75131876 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
Chr1 gene 75131719 75134851 75131719 ID=MsG0180004228.01;Name=MsG0180004228.01
Chr1 mRNA 75131719 75134798 75131719 ID=MsG0180004228.01.T01;Parent=MsG0180004228.01;Name=MsG0180004228.01.T01;_AED=0.49;_eAED=0.58;_QI=79|0.4|0.66|1|0.8|0.66|6|407|180
Chr1 exon 75131719 75131981 75131719 ID=MsG0180004228.01.T01:exon:4683;Parent=MsG0180004228.01.T01
Chr1 exon 75134295 75134448 75134295 ID=MsG0180004228.01.T01:exon:4687;Parent=MsG0180004228.01.T01
Chr1 exon 75134478 75134798 75134478 ID=MsG0180004228.01.T01:exon:4688;Parent=MsG0180004228.01.T01
Chr1 five_prime_UTR 75131719 75131797 75131719 ID=MsG0180004228.01.T01:five_prime_utr;Parent=MsG0180004228.01.T01
Chr1 CDS 75131798 75131981 75131798 ID=MsG0180004228.01.T01:cds;Parent=MsG0180004228.01.T01
Chr1 CDS 75132137 75132235 75132137 ID=MsG0180004228.01.T01:cds;Parent=MsG0180004228.01.T01
Chr1 CDS 75132327 75132403 75132327 ID=MsG0180004228.01.T01:cds;Parent=MsG0180004228.01.T01
Chr1 CDS 75133087 75133201 75133087 ID=MsG0180004228.01.T01:cds;Parent=MsG0180004228.01.T01
Chr1 CDS 75134295 75134362 75134295 ID=MsG0180004228.01.T01:cds;Parent=MsG0180004228.01.T01
Chr1 three_prime_UTR 75134363 75134448 75134363 ID=MsG0180004228.01.T01:three_prime_utr;Parent=MsG0180004228.01.T01
Chr1 three_prime_UTR 75134478 75134798 75134478 ID=MsG0180004228.01.T01:three_prime_utr;Parent=MsG0180004228.01.T01
Chr1 mRNA 75131720 75134851 75131720 ID=MsG0180004228.01.T02;Parent=MsG0180004228.01;Name=MsG0180004228.01.T02;_AED=0.49;_eAED=0.57;_QI=78|0.25|0.6|1|1|1|5|489|180
Chr1 exon 75131720 75131981 75131720 ID=MsG0180004228.01.T02:exon:4689;Parent=MsG0180004228.01.T02
Chr1 exon 75134295 75134851 75134295 ID=MsG0180004228.01.T02:exon:4690;Parent=MsG0180004228.01.T02
Chr1 five_prime_UTR 75131720 75131797 75131720 ID=MsG0180004228.01.T02:five_prime_utr;Parent=MsG0180004228.01.T02
Chr1 CDS 75131798 75131981 75131798 ID=MsG0180004228.01.T02:cds;Parent=MsG0180004228.01.T02
Chr1 CDS 75132137 75132235 75132137 ID=MsG0180004228.01.T02:cds;Parent=MsG0180004228.01.T02
Chr1 CDS 75132327 75132403 75132327 ID=MsG0180004228.01.T02:cds;Parent=MsG0180004228.01.T02
Chr1 CDS 75133087 75133201 75133087 ID=MsG0180004228.01.T02:cds;Parent=MsG0180004228.01.T02
Chr1 CDS 75134295 75134362 75134295 ID=MsG0180004228.01.T02:cds;Parent=MsG0180004228.01.T02
Chr1 three_prime_UTR 75134363 75134851 75134363 ID=MsG0180004228.01.T02:three_prime_utr;Parent=MsG0180004228.01.T02
Gene Sequence

>MsG0180004228.01.T01

ATGGGTACCCAAGACGAATCAATTGAGCAAGCCGTCGCTTCCCGCCGTGAAAGACTCCTAGCTCTTCGCGCCGCTCAACAATTATCCAATTCCTCCGAACCCGAACCCAATAATCAAGACGAAGAACAACCACAACCACCACCCACTTCTTCCGAAGACGATGATGATCAACAAGAAGAAAAGGGAAGGAAGCTTGCACCTGCTGTTCTTCCCAAATTTGAAGATCCTGTTTCTGTTCCTGACCTGAGGAAAAACCTAAAGAGGTTAGTTCAACTCTCTCTTAATCTTATGAAAACAGCTTGTGACTTGTTCATAAAGCTCTCGAAGATAGCTTATGGAAATAGATTATGGCTTTGTTTGGATCCGTTTCTCAATATTGCTCCCAAGAAACCCAACTGGGACCTGCGAAGAGATGTGCAGAAGAAGCTTGATAAGCTTGAGAAGCGAACTCAGAAGGCTCTCTATCAACTCATGGTGGAACAAGAGAAGCAAAATCAGTTAGCTGAGGAGATGATACAAACGGCGCCAAAGATTAGGTTGTAA

>MsG0180004228.01.T02

ATGGGTACCCAAGACGAATCAATTGAGCAAGCCGTCGCTTCCCGCCGTGAAAGACTCCTAGCTCTTCGCGCCGCTCAACAATTATCCAATTCCTCCGAACCCGAACCCAATAATCAAGACGAAGAACAACCACAACCACCACCCACTTCTTCCGAAGACGATGATGATCAACAAGAAGAAAAGGGAAGGAAGCTTGCACCTGCTGTTCTTCCCAAATTTGAAGATCCTGTTTCTGTTCCTGACCTGAGGAAAAACCTAAAGAGGTTAGTTCAACTCTCTCTTAATCTTATGAAAACAGCTTGTGACTTGTTCATAAAGCTCTCGAAGATAGCTTATGGAAATAGATTATGGCTTTGTTTGGATCCGTTTCTCAATATTGCTCCCAAGAAACCCAACTGGGACCTGCGAAGAGATGTGCAGAAGAAGCTTGATAAGCTTGAGAAGCGAACTCAGAAGGCTCTCTATCAACTCATGGTGGAACAAGAGAAGCAAAATCAGTTAGCTGAGGAGATGATACAAACGGCGCCAAAGATTAGGTTGTAA
Protein sequence

>MsG0180004228.01.T01

MGTQDESIEQAVASRRERLLALRAAQQLSNSSEPEPNNQDEEQPQPPPTSSEDDDDQQEEKGRKLAPAVLPKFEDPVSVPDLRKNLKRLVQLSLNLMKTACDLFIKLSKIAYGNRLWLCLDPFLNIAPKKPNWDLRRDVQKKLDKLEKRTQKALYQLMVEQEKQNQLAEEMIQTAPKIRL*

>MsG0180004228.01.T02

MGTQDESIEQAVASRRERLLALRAAQQLSNSSEPEPNNQDEEQPQPPPTSSEDDDDQQEEKGRKLAPAVLPKFEDPVSVPDLRKNLKRLVQLSLNLMKTACDLFIKLSKIAYGNRLWLCLDPFLNIAPKKPNWDLRRDVQKKLDKLEKRTQKALYQLMVEQEKQNQLAEEMIQTAPKIRL*