AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0180004355.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180004355.01.T02 MTR_1g077570 95.858 169 7 0 1 169 1 169 1.14e-113 320
MsG0180004355.01.T01 MTR_1g077570 95.858 169 7 0 1 169 1 169 1.14e-113 320
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180004355.01.T02 AT3G05070 57.669 163 43 4 1 162 1 138 1.82e-51 161
MsG0180004355.01.T01 AT3G05070 57.669 163 43 4 1 162 1 138 1.82e-51 161

Find 52 sgRNAs with CRISPR-Local

Find 169 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGTTCTTCGTCTTGATTAT+TGG 0.250780 1:-76819576 None:intergenic
GATGATCAACAAGAAGAAAA+AGG 0.261290 1:+76819632 MsG0180004355.01.T01:CDS
TGTTCTTCGTCTTGATTATT+GGG 0.276709 1:-76819575 None:intergenic
CAGCTTAGCGCATGTAAAAT+TGG 0.287549 1:+76822112 MsG0180004355.01.T01:three_prime_UTR
ACTTGTTTGATTTGAAGTTC+TGG 0.291491 1:+76822332 MsG0180004355.01.T01:three_prime_UTR
TTCGTCTTGATTATTGGGTT+CGG 0.316915 1:-76819570 None:intergenic
ACAAGTTACAACCTAATCTT+TGG 0.319987 1:-76822034 None:intergenic
AAAGAAGATTAAATTGAAAA+TGG 0.322479 1:-76822487 None:intergenic
TTGATTATTGGGTTCGGGTT+CGG 0.333205 1:-76819564 None:intergenic
TCGTCTTGATTATTGGGTTC+GGG 0.338669 1:-76819569 None:intergenic
GTTTGAACAAAAGAGGGGTT+AGG 0.377809 1:+76819383 None:intergenic
TTGCTCAATTGATTCGTCTT+CGG 0.383425 1:-76819477 None:intergenic
GGGTTCGGGTTCGGAGGAAT+TGG 0.388909 1:-76819555 None:intergenic
TTGAATTTGAGAAGAAAAGA+TGG 0.402428 1:+76819451 MsG0180004355.01.T01:exon
GAAGAAGTGGGTGGTGGTTG+TGG 0.407660 1:-76819599 None:intergenic
CAGAAAGACATCAACGCCTT+GGG 0.422509 1:-76822400 None:intergenic
TAGAATGTTTCCTCTAAAAC+AGG 0.432976 1:-76822228 None:intergenic
GCTCTATCCGATTGAATTCA+AGG 0.439363 1:+76822431 MsG0180004355.01.T01:three_prime_UTR
TTGTTGATCATCATCGTCTT+CGG 0.439513 1:-76819621 None:intergenic
TTTATTTGAGCAATCAATCA+TGG 0.445245 1:+76822517 MsG0180004355.01.T02:three_prime_UTR
ACAAGTAATATCTTTACATA+TGG 0.461634 1:-76822315 None:intergenic
TTTGAACAAAAGAGGGGTTA+GGG 0.472252 1:+76819384 None:intergenic
CGATTGAGTTTGAACAAAAG+AGG 0.475067 1:+76819376 None:intergenic
AGCGCATGTAAAATTGGAAT+CGG 0.482329 1:+76822118 MsG0180004355.01.T01:three_prime_UTR
TCAAGACCCTTGAATTCAAT+CGG 0.492932 1:-76822438 None:intergenic
TACAAACGGCGCCAAAGATT+AGG 0.500555 1:+76822023 MsG0180004355.01.T01:CDS
AGCTAGGAGTCTTTCACGGC+GGG 0.515574 1:-76819510 None:intergenic
GGATCTAACACACCCGTGCT+TGG 0.520176 1:-76822207 None:intergenic
TCTTTCACGGCGGGAAGCGA+CGG 0.522634 1:-76819501 None:intergenic
TGAATTTGAGAAGAAAAGAT+GGG 0.525345 1:+76819452 MsG0180004355.01.T01:exon
ACAGAAAGACATCAACGCCT+TGG 0.529536 1:-76822401 None:intergenic
GTTGAGCGGCGCGAAGAGCT+AGG 0.534647 1:-76819526 None:intergenic
GATTGAGTTTGAACAAAAGA+GGG 0.537468 1:+76819377 None:intergenic
TAAGCTGCTTGAATACGATA+AGG 0.540629 1:-76822096 None:intergenic
ATCATCGTCTTCGGAAGAAG+TGG 0.558826 1:-76819612 None:intergenic
TCTTGCAAAACTGCAGCAAA+TGG 0.563759 1:+76822151 MsG0180004355.01.T01:intron
CCGTGCTTGGCAAATAAAAG+AGG 0.571190 1:-76822194 None:intergenic
AAGATTAAATTGAAAATGGT+AGG 0.572470 1:-76822483 None:intergenic
ATTATTGGGTTCGGGTTCGG+AGG 0.580289 1:-76819561 None:intergenic
GAAGAGCTAGGAGTCTTTCA+CGG 0.584484 1:-76819514 None:intergenic
TCTTCGGAAGAAGTGGGTGG+TGG 0.588852 1:-76819605 None:intergenic
CTCTATCCGATTGAATTCAA+GGG 0.589025 1:+76822432 MsG0180004355.01.T01:three_prime_UTR
GAAGCAAAATCAGTTAGCTG+AGG 0.593540 1:+76821993 MsG0180004355.01.T01:CDS
CGCATGTTTAAGCTTCCCCA+AGG 0.604712 1:+76822384 MsG0180004355.01.T01:three_prime_UTR
GCTGAGGGAGATGATACAAA+CGG 0.608436 1:+76822009 MsG0180004355.01.T01:CDS
GGAATTGGATAATTGTTGAG+CGG 0.629730 1:-76819540 None:intergenic
AAGCAAAATCAGTTAGCTGA+GGG 0.635647 1:+76821994 MsG0180004355.01.T01:CDS
TCATCGTCTTCGGAAGAAGT+GGG 0.638207 1:-76819611 None:intergenic
ATTGAGTTTGAACAAAAGAG+GGG 0.656540 1:+76819378 None:intergenic
AGAAAGACATCAACGCCTTG+GGG 0.660882 1:-76822399 None:intergenic
GAGCTAGGAGTCTTTCACGG+CGG 0.702871 1:-76819511 None:intergenic
TCGTCTTCGGAAGAAGTGGG+TGG 0.708061 1:-76819608 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CTATAATAAAAGATAAAATA+TGG - Chr1:76820462-76820481 None:intergenic 10.0%
!! AAAGAAGATTAAATTGAAAA+TGG - Chr1:76822490-76822509 None:intergenic 15.0%
!! CTAATAGTACTAAAAAATTA+CGG - Chr1:76821837-76821856 None:intergenic 15.0%
!! GTCATATTTATAATTTATGA+TGG + Chr1:76821385-76821404 MsG0180004355.01.T01:intron 15.0%
!!! GATTCTAAAATAGAAATAAT+TGG + Chr1:76821622-76821641 MsG0180004355.01.T01:intron 15.0%
!!! TTTCTTATTTTCTTACTATT+TGG + Chr1:76820932-76820951 MsG0180004355.01.T01:intron 15.0%
!!! TTTTTTGAATTGAATTGAAT+TGG + Chr1:76819702-76819721 MsG0180004355.01.T01:intron 15.0%
!!! TTTTTTTAATTTTTCAACAG+TGG + Chr1:76821963-76821982 MsG0180004355.01.T01:intron 15.0%
!! AAATACTCATAAAATTGCTT+GGG - Chr1:76820664-76820683 None:intergenic 20.0%
!! AAGATTAAATTGAAAATGGT+AGG - Chr1:76822486-76822505 None:intergenic 20.0%
!! ACAAGTAATATCTTTACATA+TGG - Chr1:76822318-76822337 None:intergenic 20.0%
!! AGAAACATTATTGTAAATGT+AGG - Chr1:76820917-76820936 None:intergenic 20.0%
!! ATAAGCTATAAGATCAAAAA+AGG + Chr1:76820394-76820413 MsG0180004355.01.T01:intron 20.0%
!! CCTAATAAAAAGTAGTTAAA+AGG - Chr1:76820749-76820768 None:intergenic 20.0%
!! TAAATACTCATAAAATTGCT+TGG - Chr1:76820665-76820684 None:intergenic 20.0%
!! TAATTGCTTATGTATATTTC+TGG + Chr1:76819953-76819972 MsG0180004355.01.T01:intron 20.0%
!! TGACTAATGAATTAGTATTA+GGG + Chr1:76821564-76821583 MsG0180004355.01.T01:intron 20.0%
!! TTTGCTTAACTAATAATATC+TGG + Chr1:76821508-76821527 MsG0180004355.01.T01:intron 20.0%
!!! ATTGTTTTTGTATAAGCTAT+AGG - Chr1:76820435-76820454 None:intergenic 20.0%
!!! CCTTTTAACTACTTTTTATT+AGG + Chr1:76820746-76820765 MsG0180004355.01.T01:intron 20.0%
!!! CGTAATTTTTTAGTACTATT+AGG + Chr1:76821835-76821854 MsG0180004355.01.T01:intron 20.0%
!!! GGTGTTTTTATAAGTTATTA+TGG + Chr1:76820575-76820594 MsG0180004355.01.T01:intron 20.0%
!!! TTGTTTTTGTATAAGCTATA+GGG - Chr1:76820434-76820453 None:intergenic 20.0%
!!! TTTTTCAATTAAGAATCCAT+GGG + Chr1:76820235-76820254 MsG0180004355.01.T01:intron 20.0%
!!! TTTTTTCAATTAAGAATCCA+TGG + Chr1:76820234-76820253 MsG0180004355.01.T01:intron 20.0%
! AAAAGTAGTTAAAAGGTAAG+AGG - Chr1:76820742-76820761 None:intergenic 25.0%
! AAACAGAGTTAGAACAAAAT+TGG + Chr1:76821414-76821433 MsG0180004355.01.T01:intron 25.0%
! AGCATTTCAAAAGCAAAAAA+GGG + Chr1:76822250-76822269 MsG0180004355.01.T01:three_prime_UTR 25.0%
! ATATTCAAGAGAAAGAAAGT+AGG + Chr1:76821021-76821040 MsG0180004355.01.T01:intron 25.0%
! ATGATCAACAAGAAGAAAAA+GGG + Chr1:76819633-76819652 MsG0180004355.01.T01:CDS 25.0%
! ATTAAGCTGTTTATACAAAC+AGG + Chr1:76820521-76820540 MsG0180004355.01.T01:intron 25.0%
! CTGACTAATGAATTAGTATT+AGG + Chr1:76821563-76821582 MsG0180004355.01.T01:intron 25.0%
! GAAATTGGCAATAATACTTA+GGG - Chr1:76821744-76821763 None:intergenic 25.0%
! TAACTTATAAAAACACCTTC+CGG - Chr1:76820572-76820591 None:intergenic 25.0%
! TACTTAAGTTAATCGAAACA+AGG - Chr1:76819917-76819936 None:intergenic 25.0%
! TAGCATTTCAAAAGCAAAAA+AGG + Chr1:76822249-76822268 MsG0180004355.01.T01:three_prime_UTR 25.0%
! TAGCTTATGGAAATAGATTA+TGG + Chr1:76820047-76820066 MsG0180004355.01.T01:intron 25.0%
! TATGGATAGTCTATAGAAAT+AGG + Chr1:76820593-76820612 MsG0180004355.01.T01:intron 25.0%
! TATTCAAGAGAAAGAAAGTA+GGG + Chr1:76821022-76821041 MsG0180004355.01.T01:intron 25.0%
! TGAAATTGGCAATAATACTT+AGG - Chr1:76821745-76821764 None:intergenic 25.0%
! TGAATTTGAGAAGAAAAGAT+GGG + Chr1:76819452-76819471 MsG0180004355.01.T01:exon 25.0%
! TGTAATCATAGAATCAGAAA+TGG - Chr1:76821199-76821218 None:intergenic 25.0%
! TTGAATTTGAGAAGAAAAGA+TGG + Chr1:76819451-76819470 MsG0180004355.01.T01:exon 25.0%
! TTTATTTGAGCAATCAATCA+TGG + Chr1:76822517-76822536 MsG0180004355.01.T02:three_prime_UTR 25.0%
!! CTTATCATTAATTTTACCCA+TGG - Chr1:76820254-76820273 None:intergenic 25.0%
!! TTTGACACATTTTCAATGAA+TGG + Chr1:76820964-76820983 MsG0180004355.01.T01:intron 25.0%
!! TTTTTGCAATCAGTATTTCA+TGG - Chr1:76821228-76821247 None:intergenic 25.0%
!!! AAATAGATTATGGCTTTGTT+TGG + Chr1:76820057-76820076 MsG0180004355.01.T01:intron 25.0%
!!! TCAAATTGTTTTATACAAGC+CGG + Chr1:76820550-76820569 MsG0180004355.01.T01:intron 25.0%
ACAAGTTACAACCTAATCTT+TGG - Chr1:76822037-76822056 None:intergenic 30.0%
AGAAACAGGATCTTCAAATT+TGG - Chr1:76819842-76819861 None:intergenic 30.0%
AGAACTTAAGCTGTTATGAA+CGG + Chr1:76821111-76821130 MsG0180004355.01.T01:intron 30.0%
AGTACATACTCAGAAATCTA+TGG - Chr1:76821327-76821346 None:intergenic 30.0%
CAAGACAAAATTTCTTCTGT+AGG + Chr1:76821704-76821723 MsG0180004355.01.T01:intron 30.0%
GAAACAGGATCTTCAAATTT+GGG - Chr1:76819841-76819860 None:intergenic 30.0%
GATGATCAACAAGAAGAAAA+AGG + Chr1:76819632-76819651 MsG0180004355.01.T01:CDS 30.0%
GCATTTCAAAAGCAAAAAAG+GGG + Chr1:76822251-76822270 MsG0180004355.01.T01:three_prime_UTR 30.0%
GCTAGTTGATTGAATTTGTA+GGG + Chr1:76820137-76820156 MsG0180004355.01.T01:intron 30.0%
GTACATACTCAGAAATCTAT+GGG - Chr1:76821326-76821345 None:intergenic 30.0%
TATACAAGCAGTTAATGACA+TGG - Chr1:76820635-76820654 None:intergenic 30.0%
TGTTCTTCGTCTTGATTATT+GGG - Chr1:76819578-76819597 None:intergenic 30.0%
TTGATTGAATTTGTAGGGTT+TGG + Chr1:76820142-76820161 MsG0180004355.01.T01:intron 30.0%
TTGTTCTTCGTCTTGATTAT+TGG - Chr1:76819579-76819598 None:intergenic 30.0%
TTTGACTAAAAAGACCAGTT+TGG - Chr1:76820360-76820379 None:intergenic 30.0%
TTTGTTCTAACTCTGTTTCA+TGG - Chr1:76821412-76821431 None:intergenic 30.0%
! ATTTTCAATGAATGGTGAGT+TGG + Chr1:76820972-76820991 MsG0180004355.01.T01:intron 30.0%
! GAACTTGTGAGATTTGAAAT+TGG - Chr1:76821759-76821778 None:intergenic 30.0%
! GTAGGGTTTGGTAAAATTAA+CGG + Chr1:76820154-76820173 MsG0180004355.01.T01:intron 30.0%
! TTGAGTGATTTGAAATCTGT+CGG + Chr1:76821269-76821288 MsG0180004355.01.T01:intron 30.0%
! TTGATTTTGTTCCATGAATG+TGG + Chr1:76821918-76821937 MsG0180004355.01.T01:intron 30.0%
! TTTTCAATGAATGGTGAGTT+GGG + Chr1:76820973-76820992 MsG0180004355.01.T01:intron 30.0%
!! ACTTGTTTGATTTGAAGTTC+TGG + Chr1:76822332-76822351 MsG0180004355.01.T01:three_prime_UTR 30.0%
!! TAGAATGTTTCCTCTAAAAC+AGG - Chr1:76822231-76822250 None:intergenic 30.0%
!!! TTTTGTTGTTGTAGTGAAGA+GGG + Chr1:76819730-76819749 MsG0180004355.01.T01:intron 30.0%
!!! TTTTTGTTGTTGTAGTGAAG+AGG + Chr1:76819729-76819748 MsG0180004355.01.T01:intron 30.0%
AAAACTGTTGGATGTGGATT+AGG + Chr1:76821137-76821156 MsG0180004355.01.T01:intron 35.0%
AAGCAAAATCAGTTAGCTGA+GGG + Chr1:76821994-76822013 MsG0180004355.01.T01:CDS 35.0%
AATCCTTAACAAGTGCTCTA+AGG - Chr1:76821606-76821625 None:intergenic 35.0%
AGAGTTGAACTAACCTCTTT+AGG - Chr1:76819886-76819905 None:intergenic 35.0%
AGCGCATGTAAAATTGGAAT+CGG + Chr1:76822118-76822137 MsG0180004355.01.T01:three_prime_UTR 35.0%
ATGATAAGCATCTTCAGGAA+GGG + Chr1:76819794-76819813 MsG0180004355.01.T01:CDS 35.0%
ATTCAGAGTAAGGTCATGTT+TGG - Chr1:76820717-76820736 None:intergenic 35.0%
CTAGATAGAATAACTTCACC+TGG + Chr1:76821074-76821093 MsG0180004355.01.T01:intron 35.0%
CTATGAAAAACTATAGCCAG+CGG - Chr1:76821790-76821809 None:intergenic 35.0%
CTCTATCCGATTGAATTCAA+GGG + Chr1:76822432-76822451 MsG0180004355.01.T01:three_prime_UTR 35.0%
GGAATTGGATAATTGTTGAG+CGG - Chr1:76819543-76819562 None:intergenic 35.0%
TAAGCTGCTTGAATACGATA+AGG - Chr1:76822099-76822118 None:intergenic 35.0%
TATAAGCTATAGGGTCTGTT+TGG - Chr1:76820425-76820444 None:intergenic 35.0%
TATGAACGGATCAAAACTGT+TGG + Chr1:76821125-76821144 MsG0180004355.01.T01:intron 35.0%
TCAAGACCCTTGAATTCAAT+CGG - Chr1:76822441-76822460 None:intergenic 35.0%
TTCGTCTTGATTATTGGGTT+CGG - Chr1:76819573-76819592 None:intergenic 35.0%
TTGGGAGCAATATTGAGAAA+CGG - Chr1:76820774-76820793 None:intergenic 35.0%
TTGTTGATCATCATCGTCTT+CGG - Chr1:76819624-76819643 None:intergenic 35.0%
! GGACTTTGGAGACATTATAA+TGG + Chr1:76821939-76821958 MsG0180004355.01.T01:intron 35.0%
! GGCTAGTTGATTGAATTTGT+AGG + Chr1:76820136-76820155 MsG0180004355.01.T01:intron 35.0%
! TATCCTTAGAGCACTTGTTA+AGG + Chr1:76821600-76821619 MsG0180004355.01.T01:intron 35.0%
!! ACAAGTTCTTTTATTTCCGC+TGG + Chr1:76821771-76821790 MsG0180004355.01.T01:intron 35.0%
!! ATTGTTTTATACAAGCCGGA+AGG + Chr1:76820554-76820573 MsG0180004355.01.T01:intron 35.0%
!! TGTGTTAGATCCTGTTTTAG+AGG + Chr1:76822218-76822237 MsG0180004355.01.T01:three_prime_UTR 35.0%
!! TTGCTCAATTGATTCGTCTT+CGG - Chr1:76819480-76819499 None:intergenic 35.0%
!!! GCAAAAACACTGATTTTCTC+TGG + Chr1:76821241-76821260 MsG0180004355.01.T01:intron 35.0%
AAGCTCTCGAAGATAGCTTA+TGG + Chr1:76820034-76820053 MsG0180004355.01.T01:intron 40.0%
AAGGCTCTCTATCAACTCAT+GGG + Chr1:76820861-76820880 MsG0180004355.01.T01:CDS 40.0%
ATAAGCAGCTGTGACTGTTT+GGG + Chr1:76819977-76819996 MsG0180004355.01.T01:intron 40.0%
ATCAGCTCATGAGAAAGTGA+TGG + Chr1:76820331-76820350 MsG0180004355.01.T01:intron 40.0%
ATGACAGTGACAATGACTTG+AGG - Chr1:76821353-76821372 None:intergenic 40.0%
CAGCTTAGCGCATGTAAAAT+TGG + Chr1:76822112-76822131 MsG0180004355.01.T01:three_prime_UTR 40.0%
CATGATAAGCATCTTCAGGA+AGG + Chr1:76819793-76819812 MsG0180004355.01.T01:CDS 40.0%
CTCATAAAATTGCTTGGGAG+AGG - Chr1:76820659-76820678 None:intergenic 40.0%
GAAAGTGATGGTTACCAAAC+TGG + Chr1:76820343-76820362 MsG0180004355.01.T01:intron 40.0%
GAAGCAAAATCAGTTAGCTG+AGG + Chr1:76821993-76822012 MsG0180004355.01.T01:CDS 40.0%
GCTCTATCCGATTGAATTCA+AGG + Chr1:76822431-76822450 MsG0180004355.01.T01:three_prime_UTR 40.0%
GTAAGAGGCAATTCAGAGTA+AGG - Chr1:76820727-76820746 None:intergenic 40.0%
GTCTGAGGAAAAACCTAAAG+AGG + Chr1:76819870-76819889 MsG0180004355.01.T01:CDS 40.0%
TCAAATTTGGGAAGAACAGC+AGG - Chr1:76819829-76819848 None:intergenic 40.0%
TCAAGAGAAAGAAAGTAGGG+AGG + Chr1:76821025-76821044 MsG0180004355.01.T01:intron 40.0%
TCCTCATGATAAGCATCTTC+AGG + Chr1:76819789-76819808 MsG0180004355.01.T01:CDS 40.0%
TCCTGAAGATGCTTATCATG+AGG - Chr1:76819793-76819812 None:intergenic 40.0%
TCGTCTTGATTATTGGGTTC+GGG - Chr1:76819572-76819591 None:intergenic 40.0%
TCTTGCAAAACTGCAGCAAA+TGG + Chr1:76822151-76822170 MsG0180004355.01.T01:intron 40.0%
TGAAAGAACCTGCAAAGACA+TGG - Chr1:76821813-76821832 None:intergenic 40.0%
TGACAGTGACAATGACTTGA+GGG - Chr1:76821352-76821371 None:intergenic 40.0%
TGTTCCATGAATGTGGACTT+TGG + Chr1:76821925-76821944 MsG0180004355.01.T01:intron 40.0%
TTCTAAACTACAGCGAATCC+AGG - Chr1:76821095-76821114 None:intergenic 40.0%
TTTCATAGCCATGTCTTTGC+AGG + Chr1:76821802-76821821 MsG0180004355.01.T01:intron 40.0%
! ATGAATGGTGAGTTGGGAAA+TGG + Chr1:76820979-76820998 MsG0180004355.01.T01:intron 40.0%
! CAAGGGTCTTGAGTATTTTC+TGG + Chr1:76822449-76822468 MsG0180004355.01.T01:three_prime_UTR 40.0%
! GTTGATAAGCTAGCTTACAG+TGG + Chr1:76820103-76820122 MsG0180004355.01.T01:intron 40.0%
! TGAATGGTGAGTTGGGAAAT+GGG + Chr1:76820980-76820999 MsG0180004355.01.T01:intron 40.0%
! TTAGGTTTTTCCTCAGACTC+AGG - Chr1:76819868-76819887 None:intergenic 40.0%
! TTGATTATTGGGTTCGGGTT+CGG - Chr1:76819567-76819586 None:intergenic 40.0%
!! CCTCTTTTATTTGCCAAGCA+CGG + Chr1:76822194-76822213 MsG0180004355.01.T01:three_prime_UTR 40.0%
!! CTCTTTTATTTGCCAAGCAC+GGG + Chr1:76822195-76822214 MsG0180004355.01.T01:three_prime_UTR 40.0%
ACAGAAAGACATCAACGCCT+TGG - Chr1:76822404-76822423 None:intergenic 45.0%
AGAAAGACATCAACGCCTTG+GGG - Chr1:76822402-76822421 None:intergenic 45.0%
ATCATCGTCTTCGGAAGAAG+TGG - Chr1:76819615-76819634 None:intergenic 45.0%
CAGAAAGACATCAACGCCTT+GGG - Chr1:76822403-76822422 None:intergenic 45.0%
CATAAGCAGCTGTGACTGTT+TGG + Chr1:76819976-76819995 MsG0180004355.01.T01:intron 45.0%
CCGTGCTTGGCAAATAAAAG+AGG - Chr1:76822197-76822216 None:intergenic 45.0%
CGGATCAAAACTGTTGGATG+TGG + Chr1:76821131-76821150 MsG0180004355.01.T01:intron 45.0%
GAAGAGCTAGGAGTCTTTCA+CGG - Chr1:76819517-76819536 None:intergenic 45.0%
GAAGGCTCTCTATCAACTCA+TGG + Chr1:76820860-76820879 MsG0180004355.01.T01:CDS 45.0%
GCTGAGGGAGATGATACAAA+CGG + Chr1:76822009-76822028 MsG0180004355.01.T01:CDS 45.0%
GCTTACAGTGGATAGCGAAT+TGG + Chr1:76820115-76820134 MsG0180004355.01.T01:intron 45.0%
GTCTCCAAAGTCCACATTCA+TGG - Chr1:76821932-76821951 None:intergenic 45.0%
TACAAACGGCGCCAAAGATT+AGG + Chr1:76822023-76822042 MsG0180004355.01.T01:CDS 45.0%
TCAGACTCAGGAACAGAAAC+AGG - Chr1:76819856-76819875 None:intergenic 45.0%
TCATCGTCTTCGGAAGAAGT+GGG - Chr1:76819614-76819633 None:intergenic 45.0%
TTGTTGTAGTGAAGAGGGAG+AGG + Chr1:76819735-76819754 MsG0180004355.01.T01:intron 45.0%
! TGTTTCTGTTCCTGAGTCTG+AGG + Chr1:76819855-76819874 MsG0180004355.01.T01:CDS 45.0%
AGGTCCCAGTTGGGTTTCTT+GGG - Chr1:76820792-76820811 None:intergenic 50.0%
CGCATGTTTAAGCTTCCCCA+AGG + Chr1:76822384-76822403 MsG0180004355.01.T01:three_prime_UTR 50.0%
GCTTGAGAAGCGAACTCAGA+AGG + Chr1:76820842-76820861 MsG0180004355.01.T01:CDS 50.0%
TCTTCTGCACATCTCTTCGC+AGG - Chr1:76820812-76820831 None:intergenic 50.0%
TGCTCCCAAGAAACCCAACT+GGG + Chr1:76820785-76820804 MsG0180004355.01.T01:CDS 50.0%
TTGCTCCCAAGAAACCCAAC+TGG + Chr1:76820784-76820803 MsG0180004355.01.T01:CDS 50.0%
! ATTATTGGGTTCGGGTTCGG+AGG - Chr1:76819564-76819583 None:intergenic 50.0%
AGCTAGGAGTCTTTCACGGC+GGG - Chr1:76819513-76819532 None:intergenic 55.0%
ATCTCTTCGCAGGTCCCAGT+TGG - Chr1:76820802-76820821 None:intergenic 55.0%
CAGGTCCCAGTTGGGTTTCT+TGG - Chr1:76820793-76820812 None:intergenic 55.0%
GAGCTAGGAGTCTTTCACGG+CGG - Chr1:76819514-76819533 None:intergenic 55.0%
GGATCTAACACACCCGTGCT+TGG - Chr1:76822210-76822229 None:intergenic 55.0%
TCGTCTTCGGAAGAAGTGGG+TGG - Chr1:76819611-76819630 None:intergenic 55.0%
TCTCTTCGCAGGTCCCAGTT+GGG - Chr1:76820801-76820820 None:intergenic 55.0%
TCTTCGGAAGAAGTGGGTGG+TGG - Chr1:76819608-76819627 None:intergenic 55.0%
! GAAGAAGTGGGTGGTGGTTG+TGG - Chr1:76819602-76819621 None:intergenic 55.0%
TCTTTCACGGCGGGAAGCGA+CGG - Chr1:76819504-76819523 None:intergenic 60.0%
! GGGTTCGGGTTCGGAGGAAT+TGG - Chr1:76819558-76819577 None:intergenic 60.0%
GTTGAGCGGCGCGAAGAGCT+AGG - Chr1:76819529-76819548 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
Chr1 gene 76819387 76822545 76819387 ID=MsG0180004355.01;Name=MsG0180004355.01
Chr1 mRNA 76819387 76822491 76819387 ID=MsG0180004355.01.T01;Parent=MsG0180004355.01;Name=MsG0180004355.01.T01;_AED=0.43;_eAED=0.43;_QI=83|1|1|1|0.75|0.6|5|418|169
Chr1 exon 76819387 76819654 76819387 ID=MsG0180004355.01.T01:exon:12665;Parent=MsG0180004355.01.T01
Chr1 exon 76821983 76822137 76821983 ID=MsG0180004355.01.T01:exon:12668;Parent=MsG0180004355.01.T01
Chr1 exon 76822167 76822491 76822167 ID=MsG0180004355.01.T01:exon:12669;Parent=MsG0180004355.01.T01
Chr1 five_prime_UTR 76819387 76819469 76819387 ID=MsG0180004355.01.T01:five_prime_utr;Parent=MsG0180004355.01.T01
Chr1 CDS 76819470 76819654 76819470 ID=MsG0180004355.01.T01:cds;Parent=MsG0180004355.01.T01
Chr1 CDS 76819744 76819891 76819744 ID=MsG0180004355.01.T01:cds;Parent=MsG0180004355.01.T01
Chr1 CDS 76820768 76820882 76820768 ID=MsG0180004355.01.T01:cds;Parent=MsG0180004355.01.T01
Chr1 CDS 76821983 76822044 76821983 ID=MsG0180004355.01.T01:cds;Parent=MsG0180004355.01.T01
Chr1 three_prime_UTR 76822045 76822137 76822045 ID=MsG0180004355.01.T01:three_prime_utr;Parent=MsG0180004355.01.T01
Chr1 three_prime_UTR 76822167 76822491 76822167 ID=MsG0180004355.01.T01:three_prime_utr;Parent=MsG0180004355.01.T01
Chr1 mRNA 76819388 76822545 76819388 ID=MsG0180004355.01.T02;Parent=MsG0180004355.01;Name=MsG0180004355.01.T02;_AED=0.43;_eAED=0.43;_QI=82|1|1|1|1|1|4|501|169
Chr1 exon 76819388 76819654 76819388 ID=MsG0180004355.01.T02:exon:12670;Parent=MsG0180004355.01.T02
Chr1 exon 76821983 76822545 76821983 ID=MsG0180004355.01.T02:exon:12671;Parent=MsG0180004355.01.T02
Chr1 five_prime_UTR 76819388 76819469 76819388 ID=MsG0180004355.01.T02:five_prime_utr;Parent=MsG0180004355.01.T02
Chr1 CDS 76819470 76819654 76819470 ID=MsG0180004355.01.T02:cds;Parent=MsG0180004355.01.T02
Chr1 CDS 76819744 76819891 76819744 ID=MsG0180004355.01.T02:cds;Parent=MsG0180004355.01.T02
Chr1 CDS 76820768 76820882 76820768 ID=MsG0180004355.01.T02:cds;Parent=MsG0180004355.01.T02
Chr1 CDS 76821983 76822044 76821983 ID=MsG0180004355.01.T02:cds;Parent=MsG0180004355.01.T02
Chr1 three_prime_UTR 76822045 76822545 76822045 ID=MsG0180004355.01.T02:three_prime_utr;Parent=MsG0180004355.01.T02
Gene Sequence

>MsG0180004355.01.T02

ATGGGTGCCGAAGACGAATCAATTGAGCAAGCCGTCGCTTCCCGCCGTGAAAGACTCCTAGCTCTTCGCGCCGCTCAACAATTATCCAATTCCTCCGAACCCGAACCCAATAATCAAGACGAAGAACAACCACAACCACCACCCACTTCTTCCGAAGACGATGATGATCAACAAGAAGAAAAAGGTGAAGAGGGAGAGGAAAAGCTGAGTATGAAGTTTCGTAATTACGTTCCTCATGATAAGCATCTTCAGGAAGGGAAGCTTGCACCTGCTGTTCTTCCCAAATTTGAAGATCCTGTTTCTGTTCCTGAGTCTGAGGAAAAACCTAAAGAGGATCCGTTTCTCAATATTGCTCCCAAGAAACCCAACTGGGACCTGCGAAGAGATGTGCAGAAGAAGCTTGATAAGCTTGAGAAGCGAACTCAGAAGGCTCTCTATCAACTCATGGTGGAACAAGAGAAGCAAAATCAGTTAGCTGAGGGAGATGATACAAACGGCGCCAAAGATTAG

>MsG0180004355.01.T01

ATGGGTGCCGAAGACGAATCAATTGAGCAAGCCGTCGCTTCCCGCCGTGAAAGACTCCTAGCTCTTCGCGCCGCTCAACAATTATCCAATTCCTCCGAACCCGAACCCAATAATCAAGACGAAGAACAACCACAACCACCACCCACTTCTTCCGAAGACGATGATGATCAACAAGAAGAAAAAGGTGAAGAGGGAGAGGAAAAGCTGAGTATGAAGTTTCGTAATTACGTTCCTCATGATAAGCATCTTCAGGAAGGGAAGCTTGCACCTGCTGTTCTTCCCAAATTTGAAGATCCTGTTTCTGTTCCTGAGTCTGAGGAAAAACCTAAAGAGGATCCGTTTCTCAATATTGCTCCCAAGAAACCCAACTGGGACCTGCGAAGAGATGTGCAGAAGAAGCTTGATAAGCTTGAGAAGCGAACTCAGAAGGCTCTCTATCAACTCATGGTGGAACAAGAGAAGCAAAATCAGTTAGCTGAGGGAGATGATACAAACGGCGCCAAAGATTAG
Protein sequence

>MsG0180004355.01.T02

MGAEDESIEQAVASRRERLLALRAAQQLSNSSEPEPNNQDEEQPQPPPTSSEDDDDQQEEKGEEGEEKLSMKFRNYVPHDKHLQEGKLAPAVLPKFEDPVSVPESEEKPKEDPFLNIAPKKPNWDLRRDVQKKLDKLEKRTQKALYQLMVEQEKQNQLAEGDDTNGAKD*

>MsG0180004355.01.T01

MGAEDESIEQAVASRRERLLALRAAQQLSNSSEPEPNNQDEEQPQPPPTSSEDDDDQQEEKGEEGEEKLSMKFRNYVPHDKHLQEGKLAPAVLPKFEDPVSVPESEEKPKEDPFLNIAPKKPNWDLRRDVQKKLDKLEKRTQKALYQLMVEQEKQNQLAEGDDTNGAKD*