AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0180004439.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180004439.01.T01 MTR_1g079830 88.969 417 17 4 1 411 1 394 0.0 721
MsG0180004439.01.T01 MTR_1g079830 88.489 417 17 4 1 411 1 392 0.0 717
MsG0180004439.01.T01 MTR_1g079830 87.195 328 18 3 1 327 1 305 0.0 545
MsG0180004439.01.T01 MTR_1g079830 86.585 328 18 3 1 327 1 303 0.0 540
MsG0180004439.01.T01 MTR_1g079830 83.412 211 10 2 1 210 1 187 2.89e-115 336
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180004439.01.T01 AT3G04560 57.576 429 152 8 1 411 1 417 4.55e-147 424

Find 105 sgRNAs with CRISPR-Local

Find 200 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GATTCTTCTGCTTCTTATTA+AGG 0.223415 1:-77988257 None:intergenic
CGGCATTATTATCCTCATTT+GGG 0.253426 1:-77989380 None:intergenic
GGACTGGATTCTCTGATTCC+TGG 0.261438 1:+77989423 MsG0180004439.01.T01:CDS
TATGCAGTTATTGGTTCTAA+TGG 0.267191 1:+77987782 MsG0180004439.01.T01:intron
ACGGCATTATTATCCTCATT+TGG 0.276176 1:-77989381 None:intergenic
CAACATTGCAGCTTCTAATA+TGG 0.277849 1:-77988044 None:intergenic
AAGCAAGGAAAGTCGGTTAA+TGG 0.301650 1:+77989819 MsG0180004439.01.T01:CDS
CTAAAATATCAATTACTGTT+TGG 0.342131 1:-77989698 None:intergenic
CTAGTGTTGTAACTTATAAT+AGG 0.357215 1:+77990024 MsG0180004439.01.T01:three_prime_UTR
TCACCTTTCTTTGTGGATCC+AGG 0.371596 1:-77989441 None:intergenic
AAAATTGTGGGCTTCCATAC+TGG 0.377294 1:+77989779 MsG0180004439.01.T01:CDS
CGATTCTTCGATTGGGAAAG+AGG 0.389945 1:-77987417 None:intergenic
ACGAGTTTGCTGCTCCAGTA+TGG 0.395558 1:-77989793 None:intergenic
TTGGTAGAAACAGAAAGAAT+TGG 0.406484 1:-77987524 None:intergenic
CGGCATTCTGTTGGAGGTAG+TGG 0.408608 1:+77989906 MsG0180004439.01.T01:CDS
TCATCCTCCTCTGTCGCAAC+AGG 0.412976 1:-77989291 None:intergenic
TCGATGGCAGACGATTTGTT+TGG 0.421289 1:+77987455 MsG0180004439.01.T01:exon
ATCAGAGAATCCAGTCCAAA+TGG 0.431462 1:-77989417 None:intergenic
GAACTCGTCGATTCTTCGAT+TGG 0.432896 1:-77987425 None:intergenic
AACTCGTCGATTCTTCGATT+GGG 0.434507 1:-77987424 None:intergenic
CAATCTCAAGACAAGTTGTC+AGG 0.435206 1:-77989554 None:intergenic
GAGTAGGGTTAGGTCGTTTG+AGG 0.438043 1:-77987601 None:intergenic
TCTTAAAGATAGCACAGTTA+TGG 0.442311 1:+77989332 MsG0180004439.01.T01:CDS
TTGTCGGATTGAGTAGGGTT+AGG 0.447838 1:-77987611 None:intergenic
GTGGAGGAAGCTGACCCATT+TGG 0.448693 1:+77989402 MsG0180004439.01.T01:CDS
ATGTAGTTCTCGAAAGCAGC+TGG 0.452032 1:+77989240 MsG0180004439.01.T01:intron
GCCATTCAGCTGATTGAGGC+AGG 0.452162 1:+77987986 MsG0180004439.01.T01:CDS
AGATTATCATTCTCTGTTCA+CGG 0.452726 1:+77988102 MsG0180004439.01.T01:CDS
CGCTTCAGAAGCATCGGTCG+AGG 0.458200 1:-77987888 None:intergenic
ATATACAGATGATAGCTTTG+TGG 0.459875 1:+77988323 MsG0180004439.01.T01:CDS
GCAGCCTTGCTGAATTTCGC+AGG 0.462881 1:-77987956 None:intergenic
ATTAGCATTCGGCATTCTGT+TGG 0.467746 1:+77989897 MsG0180004439.01.T01:CDS
ATGGCAGACGATTTGTTTGG+AGG 0.477778 1:+77987458 MsG0180004439.01.T01:CDS
CGAAGGATCTGTACAAGATA+CGG 0.493375 1:-77988071 None:intergenic
TCCTGCCTCAATCAGCTGAA+TGG 0.494036 1:-77987987 None:intergenic
GTAAGTACCTTGGGAGTTGT+CGG 0.494167 1:-77987627 None:intergenic
GGGAGTTGTCGGATTGAGTA+GGG 0.497052 1:-77987616 None:intergenic
TGGGAGTTGTCGGATTGAGT+AGG 0.497207 1:-77987617 None:intergenic
TAAGAAGCAGAAGAATCAAC+TGG 0.502834 1:+77988263 MsG0180004439.01.T01:CDS
AGTAGGGTTAGGTCGTTTGA+GGG 0.510076 1:-77987600 None:intergenic
TAACTAAAATTAGCAAAGGA+AGG 0.511034 1:-77990117 None:intergenic
GGAAGCTGACCCATTTGGAC+TGG 0.513395 1:+77989407 MsG0180004439.01.T01:CDS
AGCATTCGGCATTCTGTTGG+AGG 0.514164 1:+77989900 MsG0180004439.01.T01:CDS
CGTATCTTGTACAGATCCTT+CGG 0.519093 1:+77988072 MsG0180004439.01.T01:CDS
CTACTGAGAAGATTAGCATT+CGG 0.519500 1:+77989886 MsG0180004439.01.T01:CDS
ATTCCTGGATCCACAAAGAA+AGG 0.523269 1:+77989438 MsG0180004439.01.T01:CDS
CTGTTGGAGGTAGTGGAGAC+CGG 0.524856 1:+77989913 MsG0180004439.01.T01:CDS
GAAAGCAGCTGGGCAAATCA+AGG 0.524891 1:+77989251 MsG0180004439.01.T01:CDS
GCTTGCCATTCAGCTGATTG+AGG 0.529459 1:+77987982 MsG0180004439.01.T01:CDS
GGATGCTGTAAGAAAATTGT+GGG 0.532721 1:+77989767 MsG0180004439.01.T01:CDS
ATGACGCAGCTATGAAGATA+AGG 0.534216 1:+77989481 MsG0180004439.01.T01:CDS
GAAGATAAGGAAGGAAGAAG+AGG 0.541986 1:+77989494 MsG0180004439.01.T01:CDS
CAGCCTTGCTGAATTTCGCA+GGG 0.547478 1:-77987955 None:intergenic
TCAGAGAATCCAGTCCAAAT+GGG 0.549690 1:-77989416 None:intergenic
AACTAAAATTAGCAAAGGAA+GGG 0.553978 1:-77990116 None:intergenic
AGCACAGTTATGGTAGATGA+AGG 0.558950 1:+77989342 MsG0180004439.01.T01:CDS
CGGCGTGCCACACAATGGCT+TGG 0.561588 1:+77989933 MsG0180004439.01.T01:CDS
GAGGCAGGAAGTGTTAAGTC+CGG 0.567581 1:+77988001 MsG0180004439.01.T01:CDS
ACTAAAATTAGCAAAGGAAG+GGG 0.567928 1:-77990115 None:intergenic
ACAGAAAGAATTGGAAGTTG+TGG 0.569240 1:-77987515 None:intergenic
GGCATTATTATCCTCATTTG+GGG 0.570758 1:-77989379 None:intergenic
CAGCAAACTCGTAGGAAGCA+AGG 0.571924 1:+77989804 MsG0180004439.01.T01:CDS
TAACCTTCCTGTTGCGACAG+AGG 0.572042 1:+77989284 MsG0180004439.01.T01:CDS
TGATAATCTGCTCGTACCGA+AGG 0.581963 1:-77988088 None:intergenic
GAGAATGGGTTTAGGTGCGG+AGG 0.587818 1:-77987570 None:intergenic
CAAGCCATTGTGTGGCACGC+CGG 0.591409 1:-77989932 None:intergenic
AACTTGCGCTTCAGAAGCAT+CGG 0.591974 1:-77987894 None:intergenic
TTGTGGCTGTGATTGAGTGA+TGG 0.596382 1:-77987498 None:intergenic
CAAGATTTACAGCTCATCAG+AGG 0.602405 1:+77989745 MsG0180004439.01.T01:CDS
GAAGGATCTGTACAAGATAC+GGG 0.603336 1:-77988070 None:intergenic
TGCGGAGGGAACTGAAGAAG+AGG 0.605505 1:-77987555 None:intergenic
CTTGTAACTAAAATTAGCAA+AGG 0.607904 1:-77990121 None:intergenic
CTTGTCTTGAGATTGCTGCC+CGG 0.607956 1:+77989562 MsG0180004439.01.T01:CDS
AGAATGGGTTTAGGTGCGGA+GGG 0.610754 1:-77987569 None:intergenic
TCGTAGGAAGCAAGGAAAGT+CGG 0.611363 1:+77989812 MsG0180004439.01.T01:CDS
AAGATTTACAGCTCATCAGA+GGG 0.612456 1:+77989746 MsG0180004439.01.T01:CDS
AATCTCAAGACAAGTTGTCA+GGG 0.613948 1:-77989553 None:intergenic
TCTTTCTGTTTCTACCAACA+CGG 0.616146 1:+77987529 MsG0180004439.01.T01:CDS
GGGATGCTGTAAGAAAATTG+TGG 0.616736 1:+77989766 MsG0180004439.01.T01:CDS
AACTTAACCAAGCCATTGTG+TGG 0.617182 1:-77989940 None:intergenic
TGTGATTGAGTGATGGAAGA+AGG 0.618807 1:-77987491 None:intergenic
TGTAGTTCTCGAAAGCAGCT+GGG 0.620085 1:+77989241 MsG0180004439.01.T01:intron
CCTCCTCTGTCGCAACAGGA+AGG 0.621505 1:-77989287 None:intergenic
CGCAGCTATGAAGATAAGGA+AGG 0.631678 1:+77989485 MsG0180004439.01.T01:CDS
CCTTCCTGTTGCGACAGAGG+AGG 0.632701 1:+77989287 MsG0180004439.01.T01:CDS
TTTGAGAATGGGTTTAGGTG+CGG 0.634841 1:-77987573 None:intergenic
TGAAGAAGAGGATTCCGTGT+TGG 0.637671 1:-77987543 None:intergenic
GAAGCATGCCGTTGATAATG+TGG 0.639903 1:+77989722 MsG0180004439.01.T01:CDS
CACGTGGACGATTAATGCTG+TGG 0.640827 1:+77988287 MsG0180004439.01.T01:CDS
AAATGGGTCAGCTTCCTCCA+CGG 0.642291 1:-77989400 None:intergenic
AAATCTTGCCACATTATCAA+CGG 0.643473 1:-77989730 None:intergenic
GACGAGTTCAGAACATTCGA+TGG 0.644553 1:+77987439 MsG0180004439.01.T01:exon
GAGACCGGCGTGCCACACAA+TGG 0.645448 1:+77989928 MsG0180004439.01.T01:CDS
ACTCAATCCGACAACTCCCA+AGG 0.648086 1:+77987620 MsG0180004439.01.T01:CDS
TGAGGATAATAATGCCGTGG+AGG 0.652205 1:+77989386 MsG0180004439.01.T01:CDS
GATTGAGTGATGGAAGAAGG+TGG 0.657094 1:-77987488 None:intergenic
AAATGAGGATAATAATGCCG+TGG 0.660333 1:+77989383 MsG0180004439.01.T01:CDS
ACAGTTATGGTAGATGAAGG+TGG 0.674660 1:+77989345 MsG0180004439.01.T01:CDS
GTCTTGAGATTGCTGCCCGG+CGG 0.674925 1:+77989565 MsG0180004439.01.T01:CDS
TGAGTGATGGAAGAAGGTGG+AGG 0.690751 1:-77987485 None:intergenic
GAACCCTGCGAAATTCAGCA+AGG 0.693016 1:+77987952 MsG0180004439.01.T01:CDS
TACTGGAGCAGCAAACTCGT+AGG 0.694149 1:+77989796 MsG0180004439.01.T01:CDS
CACAGCATTAATCGTCCACG+TGG 0.694480 1:-77988286 None:intergenic
GCATTATTATCCTCATTTGG+GGG 0.709961 1:-77989378 None:intergenic
AGAAGAATCAACTGGCCACG+TGG 0.729803 1:+77988271 MsG0180004439.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAATTACAAATTATATACAA+AGG - Chr1:77988524-77988543 None:intergenic 10.0%
!! AATTAATTGTCTTAATAAAA+AGG - Chr1:77988412-77988431 None:intergenic 10.0%
!!! TTAAACTTTAAACTCTTTTT+AGG - Chr1:77987866-77987885 None:intergenic 15.0%
!!! TTTTATGTTCTACAAATTTA+GGG + Chr1:77987734-77987753 MsG0180004439.01.T01:intron 15.0%
!! AAAAATTACAATTAGATCGA+TGG + Chr1:77989013-77989032 MsG0180004439.01.T01:intron 20.0%
!! AAAGAAAGGTGAAAAATTTA+AGG + Chr1:77989452-77989471 MsG0180004439.01.T01:CDS 20.0%
!! AAGAAAGGTGAAAAATTTAA+GGG + Chr1:77989453-77989472 MsG0180004439.01.T01:CDS 20.0%
!! AATGAAAAATCATCTAACTT+AGG - Chr1:77987713-77987732 None:intergenic 20.0%
!! AATTAAGATAGATTGGAAAA+AGG + Chr1:77988682-77988701 MsG0180004439.01.T01:intron 20.0%
!! ATGAAAAATCATCTAACTTA+GGG - Chr1:77987712-77987731 None:intergenic 20.0%
!! ATTCTTAATTATGCAGTTAT+TGG + Chr1:77987773-77987792 MsG0180004439.01.T01:intron 20.0%
!! CTAAAATATCAATTACTGTT+TGG - Chr1:77989701-77989720 None:intergenic 20.0%
!!! AAAAAAAAACTTTTGAGAAG+AGG - Chr1:77987392-77987411 None:intergenic 20.0%
!!! AAAAAAAACTTTTGAGAAGA+GGG - Chr1:77987391-77987410 None:intergenic 20.0%
!!! AATTTTAGTTACAAGTTTGT+CGG + Chr1:77990129-77990148 MsG0180004439.01.T01:three_prime_UTR 20.0%
!!! CTTTTATGTTCTACAAATTT+AGG + Chr1:77987733-77987752 MsG0180004439.01.T01:intron 20.0%
!!! TATCATACTCTTTAAAATGT+GGG - Chr1:77988197-77988216 None:intergenic 20.0%
!!! TTATCATACTCTTTAAAATG+TGG - Chr1:77988198-77988217 None:intergenic 20.0%
! AAAGATGAAGAGTAAATTGT+TGG - Chr1:77987673-77987692 None:intergenic 25.0%
! AAAGGAAAGAACAGATTTAT+AGG - Chr1:77988394-77988413 None:intergenic 25.0%
! AAATTGTTGGAAAAGAAAGA+AGG - Chr1:77987660-77987679 None:intergenic 25.0%
! AAATTTAGGGACTAAATTGA+TGG + Chr1:77987747-77987766 MsG0180004439.01.T01:intron 25.0%
! AACTAAAATTAGCAAAGGAA+GGG - Chr1:77990119-77990138 None:intergenic 25.0%
! ACAAGTAAACAAACATAAGA+GGG - Chr1:77988233-77988252 None:intergenic 25.0%
! ATACAGAGAAGATTCAAATA+AGG - Chr1:77989673-77989692 None:intergenic 25.0%
! CTAGTGTTGTAACTTATAAT+AGG + Chr1:77990024-77990043 MsG0180004439.01.T01:three_prime_UTR 25.0%
! CTGATTTACTTTGAATATCT+GGG + Chr1:77989168-77989187 MsG0180004439.01.T01:intron 25.0%
! CTTGTAACTAAAATTAGCAA+AGG - Chr1:77990124-77990143 None:intergenic 25.0%
! CTTGTAGAATTAAGATAGAT+TGG + Chr1:77988675-77988694 MsG0180004439.01.T01:intron 25.0%
! GAAAAAGGAATTATTCCAAT+GGG + Chr1:77988697-77988716 MsG0180004439.01.T01:intron 25.0%
! TAACTAAAATTAGCAAAGGA+AGG - Chr1:77990120-77990139 None:intergenic 25.0%
! TACAAGTAAACAAACATAAG+AGG - Chr1:77988234-77988253 None:intergenic 25.0%
! TACAGAGAAGATTCAAATAA+GGG - Chr1:77989672-77989691 None:intergenic 25.0%
! TATTTGAATCTTCTCTGTAT+AGG + Chr1:77989673-77989692 MsG0180004439.01.T01:intron 25.0%
! TTGTTACAGATGTATATTGA+GGG + Chr1:77988809-77988828 MsG0180004439.01.T01:intron 25.0%
! TTTGTTACAGATGTATATTG+AGG + Chr1:77988808-77988827 MsG0180004439.01.T01:intron 25.0%
!! AAAGTTTCTACCAATGTTTT+TGG - Chr1:77990079-77990098 None:intergenic 25.0%
!! AAATTTTTCACCTTTCTTTG+TGG - Chr1:77989451-77989470 None:intergenic 25.0%
!! AACAAATTTAAAACCCTCAT+GGG - Chr1:77989194-77989213 None:intergenic 25.0%
!! AAGTTTCTACCAATGTTTTT+GGG - Chr1:77990078-77990097 None:intergenic 25.0%
!!! ATCATACTCTTTAAAATGTG+GGG - Chr1:77988196-77988215 None:intergenic 25.0%
!!! ATTGTTTTGAAAACTTTGTC+TGG + Chr1:77989085-77989104 MsG0180004439.01.T01:intron 25.0%
AAATCTTGCCACATTATCAA+CGG - Chr1:77989733-77989752 None:intergenic 30.0%
AATTATTCCAATGGGACAAT+GGG + Chr1:77988705-77988724 MsG0180004439.01.T01:intron 30.0%
ACTAAAATTAGCAAAGGAAG+GGG - Chr1:77990118-77990137 None:intergenic 30.0%
AGATTATCATTCTCTGTTCA+CGG + Chr1:77988102-77988121 MsG0180004439.01.T01:CDS 30.0%
CCAGATATTCAAAGTAAATC+AGG - Chr1:77989170-77989189 None:intergenic 30.0%
CCTGATTTACTTTGAATATC+TGG + Chr1:77989167-77989186 MsG0180004439.01.T01:intron 30.0%
GAAAAAATAATCACTAACGC+CGG - Chr1:77988023-77988042 None:intergenic 30.0%
GATTCTTCTGCTTCTTATTA+AGG - Chr1:77988260-77988279 None:intergenic 30.0%
GGAAAAAGGAATTATTCCAA+TGG + Chr1:77988696-77988715 MsG0180004439.01.T01:intron 30.0%
TACATCTGTAACAAAAAGCA+TGG - Chr1:77988803-77988822 None:intergenic 30.0%
TACATGTATTGAGAATACAG+TGG - Chr1:77988861-77988880 None:intergenic 30.0%
TCATATCAGTATGACCAAAA+TGG + Chr1:77988603-77988622 MsG0180004439.01.T01:intron 30.0%
TCTTAAAGATAGCACAGTTA+TGG + Chr1:77989332-77989351 MsG0180004439.01.T01:CDS 30.0%
TTGGTAGAAACAGAAAGAAT+TGG - Chr1:77987527-77987546 None:intergenic 30.0%
! AATTTTCTGTAGCAGAAATG+AGG + Chr1:77989974-77989993 MsG0180004439.01.T01:three_prime_UTR 30.0%
! ATATACAGATGATAGCTTTG+TGG + Chr1:77988323-77988342 MsG0180004439.01.T01:CDS 30.0%
! CAACAAATTTAAAACCCTCA+TGG - Chr1:77989195-77989214 None:intergenic 30.0%
! GCACATTCAATTTTCATGTA+TGG - Chr1:77988484-77988503 None:intergenic 30.0%
! TGACACATTTAGATCCATTT+TGG - Chr1:77988620-77988639 None:intergenic 30.0%
! TTGATGTGAATGCTTTTGAA+TGG + Chr1:77989847-77989866 MsG0180004439.01.T01:CDS 30.0%
!! AGTTTCTACCAATGTTTTTG+GGG - Chr1:77990077-77990096 None:intergenic 30.0%
!! TATGCAGTTATTGGTTCTAA+TGG + Chr1:77987782-77987801 MsG0180004439.01.T01:intron 30.0%
!! TCATACTCTTTAAAATGTGG+GGG - Chr1:77988195-77988214 None:intergenic 30.0%
AAATGAGGATAATAATGCCG+TGG + Chr1:77989383-77989402 MsG0180004439.01.T01:CDS 35.0%
AAGATTTACAGCTCATCAGA+GGG + Chr1:77989746-77989765 MsG0180004439.01.T01:CDS 35.0%
AATCTCAAGACAAGTTGTCA+GGG - Chr1:77989556-77989575 None:intergenic 35.0%
ACAGAAAGAATTGGAAGTTG+TGG - Chr1:77987518-77987537 None:intergenic 35.0%
ACGGCATTATTATCCTCATT+TGG - Chr1:77989384-77989403 None:intergenic 35.0%
AGGAAATGATGTAAGTACCT+TGG - Chr1:77987640-77987659 None:intergenic 35.0%
CAACATTGCAGCTTCTAATA+TGG - Chr1:77988047-77988066 None:intergenic 35.0%
CATAGTAACAGCATTTACCA+AGG - Chr1:77989600-77989619 None:intergenic 35.0%
CGGCATTATTATCCTCATTT+GGG - Chr1:77989383-77989402 None:intergenic 35.0%
CTACTGAGAAGATTAGCATT+CGG + Chr1:77989886-77989905 MsG0180004439.01.T01:CDS 35.0%
GAATTATTCCAATGGGACAA+TGG + Chr1:77988704-77988723 MsG0180004439.01.T01:intron 35.0%
GCATTATTATCCTCATTTGG+GGG - Chr1:77989381-77989400 None:intergenic 35.0%
GGAAATGATGTAAGTACCTT+GGG - Chr1:77987639-77987658 None:intergenic 35.0%
GGATGCTGTAAGAAAATTGT+GGG + Chr1:77989767-77989786 MsG0180004439.01.T01:CDS 35.0%
GGCATTATTATCCTCATTTG+GGG - Chr1:77989382-77989401 None:intergenic 35.0%
TAAGAAGCAGAAGAATCAAC+TGG + Chr1:77988263-77988282 MsG0180004439.01.T01:CDS 35.0%
! CTTTGTTTCCCCAAAAACAT+TGG + Chr1:77990066-77990085 MsG0180004439.01.T01:three_prime_UTR 35.0%
! TAACTCCAACGACAAGTTTT+GGG + Chr1:77988911-77988930 MsG0180004439.01.T01:intron 35.0%
! TACTTCTTTTGCGTTTTGTG+CGG - Chr1:77988128-77988147 None:intergenic 35.0%
! TGCAACATTCTTCTTTTGAG+TGG + Chr1:77988962-77988981 MsG0180004439.01.T01:intron 35.0%
!! GAACTTTTGAGAATGGGTTT+AGG - Chr1:77987581-77987600 None:intergenic 35.0%
!! TCTTTCTGTTTCTACCAACA+CGG + Chr1:77987529-77987548 MsG0180004439.01.T01:CDS 35.0%
!! TTGAGGGAACTTTTGAGAAT+GGG - Chr1:77987587-77987606 None:intergenic 35.0%
!! TTTGAGGGAACTTTTGAGAA+TGG - Chr1:77987588-77987607 None:intergenic 35.0%
AAAATTGTGGGCTTCCATAC+TGG + Chr1:77989779-77989798 MsG0180004439.01.T01:CDS 40.0%
AACTTAACCAAGCCATTGTG+TGG - Chr1:77989943-77989962 None:intergenic 40.0%
AACTTATCCCATTGTCCCAT+TGG - Chr1:77988715-77988734 None:intergenic 40.0%
AAGCAAGGAAAGTCGGTTAA+TGG + Chr1:77989819-77989838 MsG0180004439.01.T01:CDS 40.0%
ACAGTTATGGTAGATGAAGG+TGG + Chr1:77989345-77989364 MsG0180004439.01.T01:CDS 40.0%
AGCACAGTTATGGTAGATGA+AGG + Chr1:77989342-77989361 MsG0180004439.01.T01:CDS 40.0%
ATCAGAGAATCCAGTCCAAA+TGG - Chr1:77989420-77989439 None:intergenic 40.0%
ATGACGCAGCTATGAAGATA+AGG + Chr1:77989481-77989500 MsG0180004439.01.T01:CDS 40.0%
ATTAGCATTCGGCATTCTGT+TGG + Chr1:77989897-77989916 MsG0180004439.01.T01:CDS 40.0%
ATTCCTGGATCCACAAAGAA+AGG + Chr1:77989438-77989457 MsG0180004439.01.T01:CDS 40.0%
CAAGATTTACAGCTCATCAG+AGG + Chr1:77989745-77989764 MsG0180004439.01.T01:CDS 40.0%
CAATCTCAAGACAAGTTGTC+AGG - Chr1:77989557-77989576 None:intergenic 40.0%
CAGTCGTCGGATTTAGATTT+GGG + Chr1:77989125-77989144 MsG0180004439.01.T01:intron 40.0%
CGAAGGATCTGTACAAGATA+CGG - Chr1:77988074-77988093 None:intergenic 40.0%
CGTATCTTGTACAGATCCTT+CGG + Chr1:77988072-77988091 MsG0180004439.01.T01:CDS 40.0%
GAAGATAAGGAAGGAAGAAG+AGG + Chr1:77989494-77989513 MsG0180004439.01.T01:CDS 40.0%
GAAGGATCTGTACAAGATAC+GGG - Chr1:77988073-77988092 None:intergenic 40.0%
GGACAATGGGATAAGTTAAG+TGG + Chr1:77988718-77988737 MsG0180004439.01.T01:intron 40.0%
GGGATGCTGTAAGAAAATTG+TGG + Chr1:77989766-77989785 MsG0180004439.01.T01:CDS 40.0%
TCAGAGAATCCAGTCCAAAT+GGG - Chr1:77989419-77989438 None:intergenic 40.0%
TCAGTCGTCGGATTTAGATT+TGG + Chr1:77989124-77989143 MsG0180004439.01.T01:intron 40.0%
! AACTCCAACGACAAGTTTTG+GGG + Chr1:77988912-77988931 MsG0180004439.01.T01:intron 40.0%
! CTAACTCCAACGACAAGTTT+TGG + Chr1:77988910-77988929 MsG0180004439.01.T01:intron 40.0%
! CTGTAACAAAAAGCATGGCA+TGG - Chr1:77988798-77988817 None:intergenic 40.0%
! GGGCTTTTCTTTGTAACTGA+AGG - Chr1:77989536-77989555 None:intergenic 40.0%
! GTCATCTTTTGATCAGTCGT+CGG + Chr1:77989112-77989131 MsG0180004439.01.T01:intron 40.0%
! TAAGGGCACATAATGCAAAG+TGG - Chr1:77989655-77989674 None:intergenic 40.0%
! TGTGATTGAGTGATGGAAGA+AGG - Chr1:77987494-77987513 None:intergenic 40.0%
! TTTGAGAATGGGTTTAGGTG+CGG - Chr1:77987576-77987595 None:intergenic 40.0%
!! AAACTTTTGAGAAGAGGGAG+AGG - Chr1:77987386-77987405 None:intergenic 40.0%
!! AACTCGTCGATTCTTCGATT+GGG - Chr1:77987427-77987446 None:intergenic 40.0%
!! AACTTTTGAGAAGAGGGAGA+GGG - Chr1:77987385-77987404 None:intergenic 40.0%
!! CAGATGATAGCTTTGTGGTA+TGG + Chr1:77988328-77988347 MsG0180004439.01.T01:intron 40.0%
!! GTTTTTGGGGAAACAAAGTC+AGG - Chr1:77990064-77990083 None:intergenic 40.0%
AGAAGTTTGGCACAGTTGCA+AGG + Chr1:77988939-77988958 MsG0180004439.01.T01:intron 45.0%
AGTAGGGTTAGGTCGTTTGA+GGG - Chr1:77987603-77987622 None:intergenic 45.0%
ATGTAGTTCTCGAAAGCAGC+TGG + Chr1:77989240-77989259 MsG0180004439.01.T01:intron 45.0%
CGATTCTTCGATTGGGAAAG+AGG - Chr1:77987420-77987439 None:intergenic 45.0%
CGCAGCTATGAAGATAAGGA+AGG + Chr1:77989485-77989504 MsG0180004439.01.T01:CDS 45.0%
GACGAGTTCAGAACATTCGA+TGG + Chr1:77987439-77987458 MsG0180004439.01.T01:exon 45.0%
GTAAGTACCTTGGGAGTTGT+CGG - Chr1:77987630-77987649 None:intergenic 45.0%
GTTAGTCCAGGCATCAAGAT+TGG - Chr1:77988895-77988914 None:intergenic 45.0%
TCACCTTTCTTTGTGGATCC+AGG - Chr1:77989444-77989463 None:intergenic 45.0%
TCGTAGGAAGCAAGGAAAGT+CGG + Chr1:77989812-77989831 MsG0180004439.01.T01:CDS 45.0%
TGAAGAAGAGGATTCCGTGT+TGG - Chr1:77987546-77987565 None:intergenic 45.0%
TGAGGATAATAATGCCGTGG+AGG + Chr1:77989386-77989405 MsG0180004439.01.T01:CDS 45.0%
TGATAATCTGCTCGTACCGA+AGG - Chr1:77988091-77988110 None:intergenic 45.0%
TGTAGTTCTCGAAAGCAGCT+GGG + Chr1:77989241-77989260 MsG0180004439.01.T01:intron 45.0%
TTGAATATCTGGGCCCATGA+GGG + Chr1:77989178-77989197 MsG0180004439.01.T01:intron 45.0%
TTGTCGGATTGAGTAGGGTT+AGG - Chr1:77987614-77987633 None:intergenic 45.0%
TTGTGGCTGTGATTGAGTGA+TGG - Chr1:77987501-77987520 None:intergenic 45.0%
TTTGAATATCTGGGCCCATG+AGG + Chr1:77989177-77989196 MsG0180004439.01.T01:intron 45.0%
! AACTTGCGCTTCAGAAGCAT+CGG - Chr1:77987897-77987916 None:intergenic 45.0%
! ATGGCAGACGATTTGTTTGG+AGG + Chr1:77987458-77987477 MsG0180004439.01.T01:CDS 45.0%
! GATTGAGTGATGGAAGAAGG+TGG - Chr1:77987491-77987510 None:intergenic 45.0%
! TCGATGGCAGACGATTTGTT+TGG + Chr1:77987455-77987474 MsG0180004439.01.T01:exon 45.0%
!! ACCAAGGTGTTTTATACCGC+CGG - Chr1:77989584-77989603 None:intergenic 45.0%
!! GAACTCGTCGATTCTTCGAT+TGG - Chr1:77987428-77987447 None:intergenic 45.0%
!! GAAGCATGCCGTTGATAATG+TGG + Chr1:77989722-77989741 MsG0180004439.01.T01:CDS 45.0%
!!! GTTTTGGGGTAGCAGAAGTT+TGG + Chr1:77988926-77988945 MsG0180004439.01.T01:intron 45.0%
!!! TAATTGTAATTTTTTTTAAA+AGG - Chr1:77989007-77989026 None:intergenic 5.0%
AAATGGGTCAGCTTCCTCCA+CGG - Chr1:77989403-77989422 None:intergenic 50.0%
ACTCAATCCGACAACTCCCA+AGG + Chr1:77987620-77987639 MsG0180004439.01.T01:CDS 50.0%
AGAAGAATCAACTGGCCACG+TGG + Chr1:77988271-77988290 MsG0180004439.01.T01:CDS 50.0%
AGCATTCGGCATTCTGTTGG+AGG + Chr1:77989900-77989919 MsG0180004439.01.T01:CDS 50.0%
AGTCCAGGCATCAAGATTGG+AGG - Chr1:77988892-77988911 None:intergenic 50.0%
CACAGCATTAATCGTCCACG+TGG - Chr1:77988289-77988308 None:intergenic 50.0%
CACGTGGACGATTAATGCTG+TGG + Chr1:77988287-77988306 MsG0180004439.01.T01:CDS 50.0%
CAGCAAACTCGTAGGAAGCA+AGG + Chr1:77989804-77989823 MsG0180004439.01.T01:CDS 50.0%
CAGCCTTGCTGAATTTCGCA+GGG - Chr1:77987958-77987977 None:intergenic 50.0%
CATGGCATGGATGAAGACAG+TGG - Chr1:77988785-77988804 None:intergenic 50.0%
CTTGTCGTTGGAGTTAGTCC+AGG - Chr1:77988907-77988926 None:intergenic 50.0%
CTTGTCTTGAGATTGCTGCC+CGG + Chr1:77989562-77989581 MsG0180004439.01.T01:CDS 50.0%
GAAAGCAGCTGGGCAAATCA+AGG + Chr1:77989251-77989270 MsG0180004439.01.T01:CDS 50.0%
GAACCCTGCGAAATTCAGCA+AGG + Chr1:77987952-77987971 MsG0180004439.01.T01:CDS 50.0%
GAGTAGGGTTAGGTCGTTTG+AGG - Chr1:77987604-77987623 None:intergenic 50.0%
GCTACCCCAAAACTTGTCGT+TGG - Chr1:77988919-77988938 None:intergenic 50.0%
GCTTGCCATTCAGCTGATTG+AGG + Chr1:77987982-77988001 MsG0180004439.01.T01:CDS 50.0%
GGACTGGATTCTCTGATTCC+TGG + Chr1:77989423-77989442 MsG0180004439.01.T01:CDS 50.0%
GGGAGTTGTCGGATTGAGTA+GGG - Chr1:77987619-77987638 None:intergenic 50.0%
GTCCAGGCATCAAGATTGGA+GGG - Chr1:77988891-77988910 None:intergenic 50.0%
TAACCTTCCTGTTGCGACAG+AGG + Chr1:77989284-77989303 MsG0180004439.01.T01:CDS 50.0%
TACTGGAGCAGCAAACTCGT+AGG + Chr1:77989796-77989815 MsG0180004439.01.T01:CDS 50.0%
TCAAACGTCTCCCCCAAATG+AGG + Chr1:77989368-77989387 MsG0180004439.01.T01:CDS 50.0%
TCCAGGCATCAAGATTGGAG+GGG - Chr1:77988890-77988909 None:intergenic 50.0%
TCCTGCCTCAATCAGCTGAA+TGG - Chr1:77987990-77988009 None:intergenic 50.0%
TGGGAGTTGTCGGATTGAGT+AGG - Chr1:77987620-77987639 None:intergenic 50.0%
! AGAATGGGTTTAGGTGCGGA+GGG - Chr1:77987572-77987591 None:intergenic 50.0%
! TGAGTGATGGAAGAAGGTGG+AGG - Chr1:77987488-77987507 None:intergenic 50.0%
!! ACGAGTTTGCTGCTCCAGTA+TGG - Chr1:77989796-77989815 None:intergenic 50.0%
!! CCAAGGTGTTTTATACCGCC+GGG - Chr1:77989583-77989602 None:intergenic 50.0%
!! GAGGCAGGAAGTGTTAAGTC+CGG + Chr1:77988001-77988020 MsG0180004439.01.T01:CDS 50.0%
CCAGGCATCAAGATTGGAGG+GGG - Chr1:77988889-77988908 None:intergenic 55.0%
CCCGGCGGTATAAAACACCT+TGG + Chr1:77989580-77989599 MsG0180004439.01.T01:intron 55.0%
CGGCATTCTGTTGGAGGTAG+TGG + Chr1:77989906-77989925 MsG0180004439.01.T01:CDS 55.0%
GCAGCCTTGCTGAATTTCGC+AGG - Chr1:77987959-77987978 None:intergenic 55.0%
GCCATTCAGCTGATTGAGGC+AGG + Chr1:77987986-77988005 MsG0180004439.01.T01:CDS 55.0%
GGAAGCTGACCCATTTGGAC+TGG + Chr1:77989407-77989426 MsG0180004439.01.T01:CDS 55.0%
GTGGAGGAAGCTGACCCATT+TGG + Chr1:77989402-77989421 MsG0180004439.01.T01:CDS 55.0%
TCATCCTCCTCTGTCGCAAC+AGG - Chr1:77989294-77989313 None:intergenic 55.0%
TGCGGAGGGAACTGAAGAAG+AGG - Chr1:77987558-77987577 None:intergenic 55.0%
! GAGAATGGGTTTAGGTGCGG+AGG - Chr1:77987573-77987592 None:intergenic 55.0%
!! CTGTTGGAGGTAGTGGAGAC+CGG + Chr1:77989913-77989932 MsG0180004439.01.T01:CDS 55.0%
CAAGCCATTGTGTGGCACGC+CGG - Chr1:77989935-77989954 None:intergenic 60.0%
CCCCCTCCAATCTTGATGCC+TGG + Chr1:77988886-77988905 MsG0180004439.01.T01:intron 60.0%
CCTCCTCTGTCGCAACAGGA+AGG - Chr1:77989290-77989309 None:intergenic 60.0%
CCTTCCTGTTGCGACAGAGG+AGG + Chr1:77989287-77989306 MsG0180004439.01.T01:CDS 60.0%
GTCTTGAGATTGCTGCCCGG+CGG + Chr1:77989565-77989584 MsG0180004439.01.T01:CDS 60.0%
!! CGCTTCAGAAGCATCGGTCG+AGG - Chr1:77987891-77987910 None:intergenic 60.0%
CGGCGTGCCACACAATGGCT+TGG + Chr1:77989933-77989952 MsG0180004439.01.T01:CDS 65.0%
GAGACCGGCGTGCCACACAA+TGG + Chr1:77989928-77989947 MsG0180004439.01.T01:CDS 65.0%
Chromosome Type Strat End Strand Name
Chr1 gene 77987344 77990149 77987344 ID=MsG0180004439.01;Name=MsG0180004439.01
Chr1 mRNA 77987344 77990149 77987344 ID=MsG0180004439.01.T01;Parent=MsG0180004439.01;Name=MsG0180004439.01.T01;_AED=0.31;_eAED=0.34;_QI=114|0.5|0.6|1|0.75|0.6|5|190|411
Chr1 exon 77987344 77987641 77987344 ID=MsG0180004439.01.T01:exon:12820;Parent=MsG0180004439.01.T01
Chr1 exon 77987789 77988144 77987789 ID=MsG0180004439.01.T01:exon:12821;Parent=MsG0180004439.01.T01
Chr1 exon 77988255 77988344 77988255 ID=MsG0180004439.01.T01:exon:12822;Parent=MsG0180004439.01.T01
Chr1 exon 77989246 77989586 77989246 ID=MsG0180004439.01.T01:exon:12823;Parent=MsG0180004439.01.T01
Chr1 exon 77989695 77990149 77989695 ID=MsG0180004439.01.T01:exon:12824;Parent=MsG0180004439.01.T01
Chr1 five_prime_UTR 77987344 77987457 77987344 ID=MsG0180004439.01.T01:five_prime_utr;Parent=MsG0180004439.01.T01
Chr1 CDS 77987458 77987641 77987458 ID=MsG0180004439.01.T01:cds;Parent=MsG0180004439.01.T01
Chr1 CDS 77987789 77988144 77987789 ID=MsG0180004439.01.T01:cds;Parent=MsG0180004439.01.T01
Chr1 CDS 77988255 77988344 77988255 ID=MsG0180004439.01.T01:cds;Parent=MsG0180004439.01.T01
Chr1 CDS 77989246 77989586 77989246 ID=MsG0180004439.01.T01:cds;Parent=MsG0180004439.01.T01
Chr1 CDS 77989695 77989959 77989695 ID=MsG0180004439.01.T01:cds;Parent=MsG0180004439.01.T01
Chr1 three_prime_UTR 77989960 77990149 77989960 ID=MsG0180004439.01.T01:three_prime_utr;Parent=MsG0180004439.01.T01
Gene Sequence

>MsG0180004439.01.T01

ATGGCAGACGATTTGTTTGGAGGATTGCCTCCACCTTCTTCCATCACTCAATCACAGCCACAACTTCCAATTCTTTCTGTTTCTACCAACACGGAATCCTCTTCTTCAGTTCCCTCCGCACCTAAACCCATTCTCAAAAGTTCCCTCAAACGACCTAACCCTACTCAATCCGACAACTCCCAAGTTATTGGTTCTAATGGAGTTTATATTATTGTGAAATTTAATCTTATATCTTGTATTGTAATTTCAGCTGCAGCACCTAAAAAGAGTTTAAAGTTTAAAACCTCGACCGATGCTTCTGAAGCGCAAGTTATTGACGCTATGCAGAAGATATCTTCGCATATCAAGAACCCTGCGAAATTCAGCAAGGCTGCAAAGCTTGCCATTCAGCTGATTGAGGCAGGAAGTGTTAAGTCCGGCGTTAGTGATTATTTTTTCGCCATATTAGAAGCTGCAATGTTGTCGCCCGTATCTTGTACAGATCCTTCGGTACGAGCAGATTATCATTCTCTGTTCACGGCCGCACAAAACGCAAAAGAACACCTTAATAAGAAGCAGAAGAATCAACTGGCCACGTGGACGATTAATGCTGTGGTTGCAAATGATTTATATACAGATGATAGCTTTGTGTTCTCGAAAGCAGCTGGGCAAATCAAGGAAGCTATATCTAACCTTCCTGTTGCGACAGAGGAGGATGACACAGAAGAAGCAATTTCTCTTAAAGATAGCACAGTTATGGTAGATGAAGGTGGTCAAACGTCTCCCCCAAATGAGGATAATAATGCCGTGGAGGAAGCTGACCCATTTGGACTGGATTCTCTGATTCCTGGATCCACAAAGAAAGGTGAAAAATTTAAGGGAAAGAATGACGCAGCTATGAAGATAAGGAAGGAAGAAGAGGAAGAAACTAAGCGATTCCTTCAGTTACAAAGAAAAGCCCTGACAACTTGTCTTGAGATTGCTGCCCGGCGGTGCCAAACAGTAATTGATATTTTAGTGAAGCATGCCGTTGATAATGTGGCAAGATTTACAGCTCATCAGAGGGATGCTGTAAGAAAATTGTGGGCTTCCATACTGGAGCAGCAAACTCGTAGGAAGCAAGGAAAGTCGGTTAATGGAAAGCTTGATGTGAATGCTTTTGAATGGCTTCAACAAAAATATTCTACTGAGAAGATTAGCATTCGGCATTCTGTTGGAGGTAGTGGAGACCGGCGTGCCACACAATGGCTTGGTTAA
Protein sequence

>MsG0180004439.01.T01

MADDLFGGLPPPSSITQSQPQLPILSVSTNTESSSSVPSAPKPILKSSLKRPNPTQSDNSQVIGSNGVYIIVKFNLISCIVISAAAPKKSLKFKTSTDASEAQVIDAMQKISSHIKNPAKFSKAAKLAIQLIEAGSVKSGVSDYFFAILEAAMLSPVSCTDPSVRADYHSLFTAAQNAKEHLNKKQKNQLATWTINAVVANDLYTDDSFVFSKAAGQIKEAISNLPVATEEDDTEEAISLKDSTVMVDEGGQTSPPNEDNNAVEEADPFGLDSLIPGSTKKGEKFKGKNDAAMKIRKEEEEETKRFLQLQRKALTTCLEIAARRCQTVIDILVKHAVDNVARFTAHQRDAVRKLWASILEQQTRRKQGKSVNGKLDVNAFEWLQQKYSTEKISIRHSVGGSGDRRATQWLG*