Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180004666.01.T01 | XP_013469073.1 | 92.537 | 201 | 5 | 1 | 1 | 201 | 1 | 191 | 6.16E-131 | 375 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180004666.01.T01 | O80482 | 39.801 | 201 | 86 | 5 | 1 | 180 | 2 | 188 | 1.35E-34 | 124 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180004666.01.T01 | A0A072VN91 | 92.537 | 201 | 5 | 1 | 1 | 201 | 1 | 191 | 2.94e-131 | 375 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|---|---|
| MsG0180004666.01 | MsG0280007842.01 | PPI |
| MsG0880047173.01 | MsG0180004666.01 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180004666.01.T01 | MTR_1g084160 | 92.537 | 201 | 5 | 1 | 1 | 201 | 1 | 191 | 7.46e-135 | 375 |
| MsG0180004666.01.T01 | MTR_4g122500 | 44.444 | 189 | 100 | 4 | 11 | 196 | 13 | 199 | 1.10e-41 | 140 |
| MsG0180004666.01.T01 | MTR_2g033880 | 53.642 | 151 | 63 | 3 | 42 | 187 | 45 | 193 | 5.32e-36 | 125 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180004666.01.T01 | AT1G09250 | 39.801 | 201 | 86 | 5 | 1 | 180 | 2 | 188 | 1.38e-35 | 124 |
| MsG0180004666.01.T01 | AT3G17100 | 39.487 | 195 | 103 | 2 | 4 | 183 | 12 | 206 | 7.15e-33 | 118 |
| MsG0180004666.01.T01 | AT3G17100 | 39.487 | 195 | 103 | 2 | 4 | 183 | 12 | 206 | 7.15e-33 | 118 |
| MsG0180004666.01.T01 | AT3G06590 | 46.552 | 174 | 73 | 5 | 42 | 195 | 48 | 221 | 2.16e-31 | 114 |
| MsG0180004666.01.T01 | AT3G06590 | 46.552 | 174 | 73 | 5 | 42 | 195 | 48 | 221 | 2.16e-31 | 114 |
| MsG0180004666.01.T01 | AT3G06590 | 46.552 | 174 | 73 | 5 | 42 | 195 | 48 | 221 | 2.16e-31 | 114 |
| MsG0180004666.01.T01 | AT3G06590 | 46.552 | 174 | 73 | 5 | 42 | 195 | 48 | 221 | 2.16e-31 | 114 |
| MsG0180004666.01.T01 | AT3G05800 | 44.785 | 163 | 72 | 3 | 42 | 200 | 63 | 211 | 3.72e-30 | 110 |
Find 50 sgRNAs with CRISPR-Local
Find 65 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CGCTGGAGCCGCGCGATTCT+TGG | 0.259430 | 1:+81011310 | MsG0180004666.01.T01:CDS |
| GCCGGTTAGTTCCCGGTTGC+CGG | 0.293457 | 1:+81011479 | MsG0180004666.01.T01:CDS |
| GACTTGACCGGCGGCAGGTT+TGG | 0.304053 | 1:-81011239 | None:intergenic |
| GATTCGGTAACGGAACTTTC+CGG | 0.304632 | 1:-81011498 | None:intergenic |
| CGGCAGGTTTGGTTGTTTGT+GGG | 0.307940 | 1:-81011228 | None:intergenic |
| GCCGCGCGATTCTTGGAAAA+TGG | 0.315350 | 1:+81011317 | MsG0180004666.01.T01:CDS |
| TTGTTTGTGGGGAATTTATA+CGG | 0.317650 | 1:-81011216 | None:intergenic |
| CCTCTTCAATCCGTTGTTGT+TGG | 0.328506 | 1:-81011407 | None:intergenic |
| GCGGCAGGTTTGGTTGTTTG+TGG | 0.348014 | 1:-81011229 | None:intergenic |
| GAATGAGTTGAATCTGTGTT+TGG | 0.376159 | 1:-81011052 | None:intergenic |
| TACTGATTATATTTCTGCTT+TGG | 0.379853 | 1:+81011534 | MsG0180004666.01.T01:CDS |
| TAACGGAACTTTCCGGCAAC+CGG | 0.387303 | 1:-81011491 | None:intergenic |
| CAAAAGAAAACACGCGTTCT+CGG | 0.420764 | 1:+81011457 | MsG0180004666.01.T01:CDS |
| GTTCTCGGCCGGTTAGTTCC+CGG | 0.423558 | 1:+81011472 | MsG0180004666.01.T01:CDS |
| TCCGGCAACCGGGAACTAAC+CGG | 0.427675 | 1:-81011480 | None:intergenic |
| GGACTCGATCGGCGGTTTGA+CGG | 0.439260 | 1:-81011261 | None:intergenic |
| GCCGGCGGGAATCGCCGGTC+AGG | 0.439305 | 1:+81011609 | MsG0180004666.01.T01:CDS |
| GTAGCTTCTTCTAGAAGATT+CGG | 0.450507 | 1:-81011514 | None:intergenic |
| GTCCTCGCCGCTACCGCAAA+AGG | 0.451621 | 1:+81011280 | MsG0180004666.01.T01:CDS |
| GGAGAGGATTCAACGGTTAC+CGG | 0.451742 | 1:+81011429 | MsG0180004666.01.T01:CDS |
| AGAAAACACGCGTTCTCGGC+CGG | 0.472989 | 1:+81011461 | MsG0180004666.01.T01:CDS |
| TCTCCATGGCATGAGATTGA+TGG | 0.474050 | 1:-81011143 | None:intergenic |
| CTCGCCGGTGGAACGCCGGC+GGG | 0.475976 | 1:+81011595 | MsG0180004666.01.T01:CDS |
| GATTCCCGCCGGCGTTCCAC+CGG | 0.476565 | 1:-81011599 | None:intergenic |
| AATTTATACGGAGAAGTGCT+TGG | 0.483010 | 1:-81011204 | None:intergenic |
| GGAACGCCGGCGGGAATCGC+CGG | 0.483658 | 1:+81011604 | MsG0180004666.01.T01:CDS |
| GGCGGTTTGACGGACTTGAC+CGG | 0.486922 | 1:-81011251 | None:intergenic |
| TGACGGACTTGACCGGCGGC+AGG | 0.512622 | 1:-81011244 | None:intergenic |
| ACCTGACCGGCGATTCCCGC+CGG | 0.515335 | 1:-81011610 | None:intergenic |
| TGGTCTGATTGGGATCTCCA+TGG | 0.515824 | 1:-81011157 | None:intergenic |
| GGCAGGTTTGGTTGTTTGTG+GGG | 0.516014 | 1:-81011227 | None:intergenic |
| GCTCTTGCTGAGCTTCTCGC+CGG | 0.531428 | 1:+81011580 | MsG0180004666.01.T01:CDS |
| CTTGCTGAGCTTCTCGCCGG+TGG | 0.543891 | 1:+81011583 | MsG0180004666.01.T01:CDS |
| TCTCGCCGGTGGAACGCCGG+CGG | 0.560016 | 1:+81011594 | MsG0180004666.01.T01:CDS |
| AGGTCAACTCAACACCTGAC+CGG | 0.575605 | 1:-81011623 | None:intergenic |
| AACGGAACTTTCCGGCAACC+GGG | 0.579509 | 1:-81011490 | None:intergenic |
| ACTCCATCAATCTCATGCCA+TGG | 0.583732 | 1:+81011140 | MsG0180004666.01.T01:CDS |
| CAAACAACCAAACCTGCCGC+CGG | 0.590899 | 1:+81011232 | MsG0180004666.01.T01:CDS |
| GTCAAGAAAGCTGCAAACAA+CGG | 0.591501 | 1:+81011367 | MsG0180004666.01.T01:CDS |
| GCTTCTCGCCGGTGGAACGC+CGG | 0.601420 | 1:+81011591 | MsG0180004666.01.T01:CDS |
| ACAACGGATTGAAGAGGGAG+AGG | 0.603849 | 1:+81011413 | MsG0180004666.01.T01:CDS |
| TCTTCTAGAAGATTCGGTAA+CGG | 0.605602 | 1:-81011508 | None:intergenic |
| CAACAACAACGGATTGAAGA+GGG | 0.607343 | 1:+81011408 | MsG0180004666.01.T01:CDS |
| TGAAGAGGGAGAGGATTCAA+CGG | 0.624232 | 1:+81011422 | MsG0180004666.01.T01:CDS |
| GGTAGCGGCGAGGACTCGAT+CGG | 0.629075 | 1:-81011272 | None:intergenic |
| CCAACAACAACGGATTGAAG+AGG | 0.633805 | 1:+81011407 | MsG0180004666.01.T01:CDS |
| AAGAAATCTTCCAACAACAA+CGG | 0.634484 | 1:+81011397 | MsG0180004666.01.T01:CDS |
| AGCGGCGAGGACTCGATCGG+CGG | 0.645372 | 1:-81011269 | None:intergenic |
| CCGCAAAAGGAAGAACACGC+TGG | 0.652914 | 1:+81011293 | MsG0180004666.01.T01:CDS |
| GGTTTGACGGACTTGACCGG+CGG | 0.699094 | 1:-81011248 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TACTGATTATATTTCTGCTT+TGG | + | Chr1:81011534-81011553 | MsG0180004666.01.T01:CDS | 25.0% |
| AAGAAATCTTCCAACAACAA+CGG | + | Chr1:81011397-81011416 | MsG0180004666.01.T01:CDS | 30.0% | |
| TTGTTTGTGGGGAATTTATA+CGG | - | Chr1:81011219-81011238 | None:intergenic | 30.0% | |
| !!! | TGACTTTTTTGTGATGTCTT+CGG | - | Chr1:81011351-81011370 | None:intergenic | 30.0% |
| ! | GAATGAGTTGAATCTGTGTT+TGG | - | Chr1:81011055-81011074 | None:intergenic | 35.0% |
| ! | GTAGCTTCTTCTAGAAGATT+CGG | - | Chr1:81011517-81011536 | None:intergenic | 35.0% |
| ! | TCTTCTAGAAGATTCGGTAA+CGG | - | Chr1:81011511-81011530 | None:intergenic | 35.0% |
| !! | AATTTATACGGAGAAGTGCT+TGG | - | Chr1:81011207-81011226 | None:intergenic | 35.0% |
| !! | GAGATTGATGGAGTTTTGAT+CGG | - | Chr1:81011134-81011153 | None:intergenic | 35.0% |
| !!! | AAGTGCTTGGATGAGTTTTT+TGG | - | Chr1:81011194-81011213 | None:intergenic | 35.0% |
| !!! | GTTTTTTGGAGTAGGTGTTT+TGG | - | Chr1:81011180-81011199 | None:intergenic | 35.0% |
| !!! | TTGGATGAGTTTTTTGGAGT+AGG | - | Chr1:81011188-81011207 | None:intergenic | 35.0% |
| CAAAAGAAAACACGCGTTCT+CGG | + | Chr1:81011457-81011476 | MsG0180004666.01.T01:CDS | 40.0% | |
| GTCAAGAAAGCTGCAAACAA+CGG | + | Chr1:81011367-81011386 | MsG0180004666.01.T01:CDS | 40.0% | |
| !! | ATTGATGGAGTTTTGATCGG+TGG | - | Chr1:81011131-81011150 | None:intergenic | 40.0% |
| !! | CAACAACAACGGATTGAAGA+GGG | + | Chr1:81011408-81011427 | MsG0180004666.01.T01:CDS | 40.0% |
| !!! | AGTAGGTGTTTTGGTCTGAT+TGG | - | Chr1:81011171-81011190 | None:intergenic | 40.0% |
| !!! | GTAGGTGTTTTGGTCTGATT+GGG | - | Chr1:81011170-81011189 | None:intergenic | 40.0% |
| !!! | TGGTGAGGTTATCGATTTTG+CGG | - | Chr1:81011111-81011130 | None:intergenic | 40.0% |
| !!! | TTTGCGGCGTTTTTTGTGAT+TGG | - | Chr1:81011095-81011114 | None:intergenic | 40.0% |
| ACTCCATCAATCTCATGCCA+TGG | + | Chr1:81011140-81011159 | MsG0180004666.01.T01:CDS | 45.0% | |
| CCTCTTCAATCCGTTGTTGT+TGG | - | Chr1:81011410-81011429 | None:intergenic | 45.0% | |
| TCTCCATGGCATGAGATTGA+TGG | - | Chr1:81011146-81011165 | None:intergenic | 45.0% | |
| TGAAGAGGGAGAGGATTCAA+CGG | + | Chr1:81011422-81011441 | MsG0180004666.01.T01:CDS | 45.0% | |
| ! | CGTGTTTTCTTTTGCACAGC+CGG | - | Chr1:81011451-81011470 | None:intergenic | 45.0% |
| ! | TCCATTTTCCAAGAATCGCG+CGG | - | Chr1:81011321-81011340 | None:intergenic | 45.0% |
| ! | TGTTCTTCCTTTTGCGGTAG+CGG | - | Chr1:81011290-81011309 | None:intergenic | 45.0% |
| !! | CCAACAACAACGGATTGAAG+AGG | + | Chr1:81011407-81011426 | MsG0180004666.01.T01:CDS | 45.0% |
| !! | GATTCGGTAACGGAACTTTC+CGG | - | Chr1:81011501-81011520 | None:intergenic | 45.0% |
| GGAGAGGATTCAACGGTTAC+CGG | + | Chr1:81011429-81011448 | MsG0180004666.01.T01:CDS | 50.0% | |
| TAACGGAACTTTCCGGCAAC+CGG | - | Chr1:81011494-81011513 | None:intergenic | 50.0% | |
| TGGTCTGATTGGGATCTCCA+TGG | - | Chr1:81011160-81011179 | None:intergenic | 50.0% | |
| ! | CCAGCGTGTTCTTCCTTTTG+CGG | - | Chr1:81011296-81011315 | None:intergenic | 50.0% |
| ! | CGGCAGGTTTGGTTGTTTGT+GGG | - | Chr1:81011231-81011250 | None:intergenic | 50.0% |
| ! | GGCAGGTTTGGTTGTTTGTG+GGG | - | Chr1:81011230-81011249 | None:intergenic | 50.0% |
| !! | ACAACGGATTGAAGAGGGAG+AGG | + | Chr1:81011413-81011432 | MsG0180004666.01.T01:CDS | 50.0% |
| !! | TGGAGTTTTGATCGGTGGTG+AGG | - | Chr1:81011126-81011145 | None:intergenic | 50.0% |
| AACGGAACTTTCCGGCAACC+GGG | - | Chr1:81011493-81011512 | None:intergenic | 55.0% | |
| AGAAAACACGCGTTCTCGGC+CGG | + | Chr1:81011461-81011480 | MsG0180004666.01.T01:CDS | 55.0% | |
| CAAACAACCAAACCTGCCGC+CGG | + | Chr1:81011232-81011251 | MsG0180004666.01.T01:CDS | 55.0% | |
| CCGCAAAAGGAAGAACACGC+TGG | + | Chr1:81011293-81011312 | MsG0180004666.01.T01:CDS | 55.0% | |
| GCCGCGCGATTCTTGGAAAA+TGG | + | Chr1:81011317-81011336 | MsG0180004666.01.T01:CDS | 55.0% | |
| ! | GCGGCAGGTTTGGTTGTTTG+TGG | - | Chr1:81011232-81011251 | None:intergenic | 55.0% |
| GCTCTTGCTGAGCTTCTCGC+CGG | + | Chr1:81011580-81011599 | MsG0180004666.01.T01:CDS | 60.0% | |
| GGACTCGATCGGCGGTTTGA+CGG | - | Chr1:81011264-81011283 | None:intergenic | 60.0% | |
| TCCGGCAACCGGGAACTAAC+CGG | - | Chr1:81011483-81011502 | None:intergenic | 60.0% | |
| ! | GTTCTCGGCCGGTTAGTTCC+CGG | + | Chr1:81011472-81011491 | MsG0180004666.01.T01:CDS | 60.0% |
| !! | GGCGGTTTGACGGACTTGAC+CGG | - | Chr1:81011254-81011273 | None:intergenic | 60.0% |
| !! | GGTTTGACGGACTTGACCGG+CGG | - | Chr1:81011251-81011270 | None:intergenic | 60.0% |
| !! | TTCCTTTTGCGGTAGCGGCG+AGG | - | Chr1:81011285-81011304 | None:intergenic | 60.0% |
| CTTGCTGAGCTTCTCGCCGG+TGG | + | Chr1:81011583-81011602 | MsG0180004666.01.T01:CDS | 65.0% | |
| GACTTGACCGGCGGCAGGTT+TGG | - | Chr1:81011242-81011261 | None:intergenic | 65.0% | |
| GTCCTCGCCGCTACCGCAAA+AGG | + | Chr1:81011280-81011299 | MsG0180004666.01.T01:CDS | 65.0% | |
| ! | GCCGGTTAGTTCCCGGTTGC+CGG | + | Chr1:81011479-81011498 | MsG0180004666.01.T01:CDS | 65.0% |
| ! | GGTAGCGGCGAGGACTCGAT+CGG | - | Chr1:81011275-81011294 | None:intergenic | 65.0% |
| ACCTGACCGGCGATTCCCGC+CGG | - | Chr1:81011613-81011632 | None:intergenic | 70.0% | |
| AGCGGCGAGGACTCGATCGG+CGG | - | Chr1:81011272-81011291 | None:intergenic | 70.0% | |
| CGCTGGAGCCGCGCGATTCT+TGG | + | Chr1:81011310-81011329 | MsG0180004666.01.T01:CDS | 70.0% | |
| GATTCCCGCCGGCGTTCCAC+CGG | - | Chr1:81011602-81011621 | None:intergenic | 70.0% | |
| GCTTCTCGCCGGTGGAACGC+CGG | + | Chr1:81011591-81011610 | MsG0180004666.01.T01:CDS | 70.0% | |
| !! | TGACGGACTTGACCGGCGGC+AGG | - | Chr1:81011247-81011266 | None:intergenic | 70.0% |
| GGAACGCCGGCGGGAATCGC+CGG | + | Chr1:81011604-81011623 | MsG0180004666.01.T01:CDS | 75.0% | |
| TCTCGCCGGTGGAACGCCGG+CGG | + | Chr1:81011594-81011613 | MsG0180004666.01.T01:CDS | 75.0% | |
| ! | CTCGCCGGTGGAACGCCGGC+GGG | + | Chr1:81011595-81011614 | MsG0180004666.01.T01:CDS | 80.0% |
| ! | GCCGGCGGGAATCGCCGGTC+AGG | + | Chr1:81011609-81011628 | MsG0180004666.01.T01:CDS | 80.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr1 | gene | 81011037 | 81011642 | 81011037 | ID=MsG0180004666.01;Name=MsG0180004666.01 |
| Chr1 | mRNA | 81011037 | 81011642 | 81011037 | ID=MsG0180004666.01.T01;Parent=MsG0180004666.01;Name=MsG0180004666.01.T01;_AED=0.25;_eAED=0.25;_QI=0|-1|0|1|-1|1|1|0|201 |
| Chr1 | exon | 81011037 | 81011642 | 81011037 | ID=MsG0180004666.01.T01:exon:15703;Parent=MsG0180004666.01.T01 |
| Chr1 | CDS | 81011037 | 81011642 | 81011037 | ID=MsG0180004666.01.T01:cds;Parent=MsG0180004666.01.T01 |
| Gene Sequence |
| Protein sequence |
