Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880047173.01.T01 | XP_003609794.1 | 97.525 | 202 | 4 | 1 | 1 | 202 | 1 | 201 | 1.17E-138 | 395 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880047173.01.T01 | Q9C8Z9 | 54.545 | 176 | 60 | 4 | 40 | 196 | 43 | 217 | 3.31E-46 | 154 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880047173.01.T01 | G7JNY4 | 97.525 | 202 | 4 | 1 | 1 | 202 | 1 | 201 | 5.57e-139 | 395 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI
| Gene1 | Gene2 | Type |
|---|---|---|
| MsG0880047173.01 | MsG0180004666.01 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880047173.01.T01 | MTR_4g122500 | 97.525 | 202 | 4 | 1 | 1 | 202 | 1 | 201 | 1.41e-142 | 395 |
| MsG0880047173.01.T01 | MTR_2g033880 | 61.224 | 196 | 67 | 5 | 1 | 190 | 1 | 193 | 4.72e-56 | 177 |
| MsG0880047173.01.T01 | MTR_1g084160 | 46.316 | 190 | 86 | 5 | 13 | 200 | 11 | 186 | 2.92e-43 | 143 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0880047173.01.T01 | AT3G06590 | 54.545 | 176 | 60 | 4 | 40 | 196 | 43 | 217 | 3.37e-47 | 154 |
| MsG0880047173.01.T01 | AT3G06590 | 54.545 | 176 | 60 | 4 | 40 | 196 | 43 | 217 | 3.37e-47 | 154 |
| MsG0880047173.01.T01 | AT3G06590 | 54.545 | 176 | 60 | 4 | 40 | 196 | 43 | 217 | 3.37e-47 | 154 |
| MsG0880047173.01.T01 | AT3G06590 | 54.545 | 176 | 60 | 4 | 40 | 196 | 43 | 217 | 3.37e-47 | 154 |
| MsG0880047173.01.T01 | AT3G17100 | 54.037 | 161 | 57 | 3 | 44 | 188 | 49 | 208 | 3.04e-45 | 150 |
| MsG0880047173.01.T01 | AT3G17100 | 54.037 | 161 | 57 | 3 | 44 | 188 | 49 | 208 | 3.04e-45 | 150 |
| MsG0880047173.01.T01 | AT3G05800 | 41.447 | 152 | 79 | 2 | 40 | 191 | 58 | 199 | 3.63e-32 | 115 |
| MsG0880047173.01.T01 | AT1G09250 | 43.709 | 151 | 69 | 4 | 44 | 183 | 43 | 188 | 1.15e-29 | 109 |
Find 57 sgRNAs with CRISPR-Local
Find 68 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGCGCTTGGGATTTCCATTT+GGG | 0.233280 | 8:-82950839 | None:intergenic |
| CTGCGCTTGGGATTTCCATT+TGG | 0.249430 | 8:-82950840 | None:intergenic |
| GGCAACGCCGTTACCCTATT+AGG | 0.286717 | 8:-82951049 | None:intergenic |
| CTTCTTTCTGCTTCTACTTC+CGG | 0.297918 | 8:+82951277 | MsG0880047173.01.T01:CDS |
| TTTCTTGGAGGGTTAGTTCC+TGG | 0.310377 | 8:+82951157 | MsG0880047173.01.T01:CDS |
| CAGAGGAAAGTAAGGTTTCT+TGG | 0.324461 | 8:+82951142 | MsG0880047173.01.T01:CDS |
| GTTAGTTCCTGGTTGCAAAA+AGG | 0.345775 | 8:+82951168 | MsG0880047173.01.T01:CDS |
| TCTGTGTTTGTTACTATGTT+TGG | 0.347425 | 8:-82950737 | None:intergenic |
| TCCGGCGCCGGCGTCAGTTC+CGG | 0.348417 | 8:+82951295 | MsG0880047173.01.T01:CDS |
| TGATGAGTTGACTCTTGTTA+TGG | 0.377921 | 8:-82950894 | None:intergenic |
| GACTTCTTTGACCTACTTGA+CGG | 0.395279 | 8:-82951070 | None:intergenic |
| AGTAGATTTGTTGCTGCGCT+TGG | 0.418456 | 8:-82950853 | None:intergenic |
| TCTTCAAGAATCACTGGTAA+TGG | 0.430797 | 8:-82951193 | None:intergenic |
| CTTACTTTCCTCTGCACACT+TGG | 0.435034 | 8:-82951133 | None:intergenic |
| TGTTATGGCTTGACGGAGCT+TGG | 0.440768 | 8:-82950879 | None:intergenic |
| TGACTCTTGTTATGGCTTGA+CGG | 0.450930 | 8:-82950886 | None:intergenic |
| TATGATGATATCTTATCAAC+CGG | 0.452874 | 8:-82951314 | None:intergenic |
| GAGGAAGAACTCGTTGGAGC+CGG | 0.456746 | 8:+82950981 | MsG0880047173.01.T01:CDS |
| TATACCTGCACTTGAGATGC+AGG | 0.461665 | 8:+82951228 | MsG0880047173.01.T01:CDS |
| CCTAAACTTGATCTTGAGCC+GGG | 0.461676 | 8:-82951017 | None:intergenic |
| TCCTAAACTTGATCTTGAGC+CGG | 0.463544 | 8:-82951018 | None:intergenic |
| AAATTCAAATCAACCCCAAA+TGG | 0.465919 | 8:+82950825 | MsG0880047173.01.T01:CDS |
| ATGTTTGGTATTGTAGTAGA+TGG | 0.485128 | 8:-82950722 | None:intergenic |
| TCTGCTTCTACTTCCGGCGC+CGG | 0.492364 | 8:+82951283 | MsG0880047173.01.T01:CDS |
| GTAGATTTGTTGCTGCGCTT+GGG | 0.492934 | 8:-82950852 | None:intergenic |
| CCACCACCTTTCCCTTCAAA+CGG | 0.497609 | 8:-82951108 | None:intergenic |
| CCGTTTGAAGGGAAAGGTGG+TGG | 0.499437 | 8:+82951108 | MsG0880047173.01.T01:CDS |
| AGGAAGAACTCGTTGGAGCC+GGG | 0.501516 | 8:+82950982 | MsG0880047173.01.T01:CDS |
| CCCGGCTCAAGATCAAGTTT+AGG | 0.508626 | 8:+82951017 | MsG0880047173.01.T01:CDS |
| CAAGTGTGCAGAGGAAAGTA+AGG | 0.512788 | 8:+82951134 | MsG0880047173.01.T01:CDS |
| ATAGCTTCTTCAAGAATCAC+TGG | 0.515421 | 8:-82951199 | None:intergenic |
| AGAAGCCGCTGATAGAGCTC+TGG | 0.527592 | 8:+82950946 | MsG0880047173.01.T01:CDS |
| GCTCCAACGAGTTCTTCCTC+TGG | 0.528843 | 8:-82950978 | None:intergenic |
| ACCGGAACTGACGCCGGCGC+CGG | 0.528888 | 8:-82951296 | None:intergenic |
| GGAAAGTAAGGTTTCTTGGA+GGG | 0.531091 | 8:+82951146 | MsG0880047173.01.T01:CDS |
| CGGAAGTAGAAGCAGAAAGA+AGG | 0.543119 | 8:-82951276 | None:intergenic |
| CTTGAGCCGGGTCATTAGAA+TGG | 0.544770 | 8:-82951005 | None:intergenic |
| GCCGGGCCATTCTAATGACC+CGG | 0.550445 | 8:+82950999 | MsG0880047173.01.T01:CDS |
| CAAGTAGGTCAAAGAAGTCA+AGG | 0.557631 | 8:+82951074 | MsG0880047173.01.T01:CDS |
| CTAACCTGCATCTCAAGTGC+AGG | 0.560755 | 8:-82951232 | None:intergenic |
| TATGGCTTGACGGAGCTTGG+TGG | 0.564034 | 8:-82950876 | None:intergenic |
| GCCGGGTCATTAGAATGGCC+CGG | 0.573825 | 8:-82951000 | None:intergenic |
| GCTCTGGCCGTGACGGCCAG+AGG | 0.576086 | 8:+82950962 | MsG0880047173.01.T01:CDS |
| TAACGGCGTTGCCGTCAAGT+AGG | 0.589997 | 8:+82951059 | MsG0880047173.01.T01:CDS |
| CGGCCAGAGGAAGAACTCGT+TGG | 0.595590 | 8:+82950975 | MsG0880047173.01.T01:CDS |
| AGTTCTTCCTCTGGCCGTCA+CGG | 0.603583 | 8:-82950969 | None:intergenic |
| TCTATCAGCGGCTTCTCGAA+CGG | 0.607548 | 8:-82950939 | None:intergenic |
| TGATAGAGCTCTGGCCGTGA+CGG | 0.617790 | 8:+82950955 | MsG0880047173.01.T01:CDS |
| TTTCCGTTTGAAGGGAAAGG+TGG | 0.617820 | 8:+82951105 | MsG0880047173.01.T01:CDS |
| AGGAAAGTAAGGTTTCTTGG+AGG | 0.618132 | 8:+82951145 | MsG0880047173.01.T01:CDS |
| TCATCATCCACCCCGCGCCG+AGG | 0.627296 | 8:+82950911 | MsG0880047173.01.T01:CDS |
| TTATCAACCGGAACTGACGC+CGG | 0.631904 | 8:-82951302 | None:intergenic |
| CAGACATATCACGTGACACA+AGG | 0.632446 | 8:+82950756 | MsG0880047173.01.T01:CDS |
| GCGCTTGGGATTTCCATTTG+GGG | 0.639024 | 8:-82950838 | None:intergenic |
| AAGTAGGTCAAAGAAGTCAA+GGG | 0.645774 | 8:+82951075 | MsG0880047173.01.T01:CDS |
| TGGTGGTACCAAGTGTGCAG+AGG | 0.653274 | 8:+82951125 | MsG0880047173.01.T01:CDS |
| CACGGCCAGAGCTCTATCAG+CGG | 0.727484 | 8:-82950951 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | TAGGAAGAAAAAACCTAATA+GGG | + | Chr8:82951036-82951055 | MsG0880047173.01.T01:CDS | 25.0% |
| ! | TTAGGAAGAAAAAACCTAAT+AGG | + | Chr8:82951035-82951054 | MsG0880047173.01.T01:CDS | 25.0% |
| AAATTCAAATCAACCCCAAA+TGG | + | Chr8:82950825-82950844 | MsG0880047173.01.T01:CDS | 30.0% | |
| ATGTTTGGTATTGTAGTAGA+TGG | - | Chr8:82950725-82950744 | None:intergenic | 30.0% | |
| GAAAAAACCTAATAGGGTAA+CGG | + | Chr8:82951042-82951061 | MsG0880047173.01.T01:CDS | 30.0% | |
| ! | TCTGTGTTTGTTACTATGTT+TGG | - | Chr8:82950740-82950759 | None:intergenic | 30.0% |
| AAGTAGGTCAAAGAAGTCAA+GGG | + | Chr8:82951075-82951094 | MsG0880047173.01.T01:CDS | 35.0% | |
| ATAGCTTCTTCAAGAATCAC+TGG | - | Chr8:82951202-82951221 | None:intergenic | 35.0% | |
| TCTTCAAGAATCACTGGTAA+TGG | - | Chr8:82951196-82951215 | None:intergenic | 35.0% | |
| ! | TGATGAGTTGACTCTTGTTA+TGG | - | Chr8:82950897-82950916 | None:intergenic | 35.0% |
| !! | AAGGTTAAAAAGAGCACTCA+TGG | - | Chr8:82951260-82951279 | None:intergenic | 35.0% |
| AGGAAAGTAAGGTTTCTTGG+AGG | + | Chr8:82951145-82951164 | MsG0880047173.01.T01:CDS | 40.0% | |
| CAAGTAGGTCAAAGAAGTCA+AGG | + | Chr8:82951074-82951093 | MsG0880047173.01.T01:CDS | 40.0% | |
| CAGAGGAAAGTAAGGTTTCT+TGG | + | Chr8:82951142-82951161 | MsG0880047173.01.T01:CDS | 40.0% | |
| CTTCTTTCTGCTTCTACTTC+CGG | + | Chr8:82951277-82951296 | MsG0880047173.01.T01:CDS | 40.0% | |
| GACTTCTTTGACCTACTTGA+CGG | - | Chr8:82951073-82951092 | None:intergenic | 40.0% | |
| GGAAAGTAAGGTTTCTTGGA+GGG | + | Chr8:82951146-82951165 | MsG0880047173.01.T01:CDS | 40.0% | |
| GTTAGTTCCTGGTTGCAAAA+AGG | + | Chr8:82951168-82951187 | MsG0880047173.01.T01:CDS | 40.0% | |
| TCCTAAACTTGATCTTGAGC+CGG | - | Chr8:82951021-82951040 | None:intergenic | 40.0% | |
| ! | AATGGTTCCTTTTTGCAACC+AGG | - | Chr8:82951178-82951197 | None:intergenic | 40.0% |
| ! | GTCAACGTTTTCCGTTTGAA+GGG | + | Chr8:82951097-82951116 | MsG0880047173.01.T01:CDS | 40.0% |
| ! | TGACTCTTGTTATGGCTTGA+CGG | - | Chr8:82950889-82950908 | None:intergenic | 40.0% |
| CAAGTGTGCAGAGGAAAGTA+AGG | + | Chr8:82951134-82951153 | MsG0880047173.01.T01:CDS | 45.0% | |
| CAGACATATCACGTGACACA+AGG | + | Chr8:82950756-82950775 | MsG0880047173.01.T01:CDS | 45.0% | |
| CCTAAACTTGATCTTGAGCC+GGG | - | Chr8:82951020-82951039 | None:intergenic | 45.0% | |
| CGGAAGTAGAAGCAGAAAGA+AGG | - | Chr8:82951279-82951298 | None:intergenic | 45.0% | |
| CTTACTTTCCTCTGCACACT+TGG | - | Chr8:82951136-82951155 | None:intergenic | 45.0% | |
| TATACCTGCACTTGAGATGC+AGG | + | Chr8:82951228-82951247 | MsG0880047173.01.T01:CDS | 45.0% | |
| TGCGCTTGGGATTTCCATTT+GGG | - | Chr8:82950842-82950861 | None:intergenic | 45.0% | |
| TTTCCGTTTGAAGGGAAAGG+TGG | + | Chr8:82951105-82951124 | MsG0880047173.01.T01:CDS | 45.0% | |
| ! | CGTTTTCCGTTTGAAGGGAA+AGG | + | Chr8:82951102-82951121 | MsG0880047173.01.T01:CDS | 45.0% |
| ! | GGTCAACGTTTTCCGTTTGA+AGG | + | Chr8:82951096-82951115 | MsG0880047173.01.T01:CDS | 45.0% |
| !! | AGTAGATTTGTTGCTGCGCT+TGG | - | Chr8:82950856-82950875 | None:intergenic | 45.0% |
| !! | GTAGATTTGTTGCTGCGCTT+GGG | - | Chr8:82950855-82950874 | None:intergenic | 45.0% |
| !! | TTTCTTGGAGGGTTAGTTCC+TGG | + | Chr8:82951157-82951176 | MsG0880047173.01.T01:CDS | 45.0% |
| CCACCACCTTTCCCTTCAAA+CGG | - | Chr8:82951111-82951130 | None:intergenic | 50.0% | |
| CCCGGCTCAAGATCAAGTTT+AGG | + | Chr8:82951017-82951036 | MsG0880047173.01.T01:CDS | 50.0% | |
| CTAACCTGCATCTCAAGTGC+AGG | - | Chr8:82951235-82951254 | None:intergenic | 50.0% | |
| CTGCGCTTGGGATTTCCATT+TGG | - | Chr8:82950843-82950862 | None:intergenic | 50.0% | |
| CTTGAGCCGGGTCATTAGAA+TGG | - | Chr8:82951008-82951027 | None:intergenic | 50.0% | |
| GCGCTTGGGATTTCCATTTG+GGG | - | Chr8:82950841-82950860 | None:intergenic | 50.0% | |
| TCTATCAGCGGCTTCTCGAA+CGG | - | Chr8:82950942-82950961 | None:intergenic | 50.0% | |
| TGTTATGGCTTGACGGAGCT+TGG | - | Chr8:82950882-82950901 | None:intergenic | 50.0% | |
| ! | CTTCTCGAACGGCTTTTCCT+CGG | - | Chr8:82950931-82950950 | None:intergenic | 50.0% |
| AGAAGCCGCTGATAGAGCTC+TGG | + | Chr8:82950946-82950965 | MsG0880047173.01.T01:CDS | 55.0% | |
| AGGAAGAACTCGTTGGAGCC+GGG | + | Chr8:82950982-82951001 | MsG0880047173.01.T01:CDS | 55.0% | |
| AGTTCTTCCTCTGGCCGTCA+CGG | - | Chr8:82950972-82950991 | None:intergenic | 55.0% | |
| CCGTTTGAAGGGAAAGGTGG+TGG | + | Chr8:82951108-82951127 | MsG0880047173.01.T01:CDS | 55.0% | |
| GAGGAAGAACTCGTTGGAGC+CGG | + | Chr8:82950981-82951000 | MsG0880047173.01.T01:CDS | 55.0% | |
| GCTCCAACGAGTTCTTCCTC+TGG | - | Chr8:82950981-82951000 | None:intergenic | 55.0% | |
| GGCAACGCCGTTACCCTATT+AGG | - | Chr8:82951052-82951071 | None:intergenic | 55.0% | |
| TAACGGCGTTGCCGTCAAGT+AGG | + | Chr8:82951059-82951078 | MsG0880047173.01.T01:CDS | 55.0% | |
| TATGGCTTGACGGAGCTTGG+TGG | - | Chr8:82950879-82950898 | None:intergenic | 55.0% | |
| TGGTGGTACCAAGTGTGCAG+AGG | + | Chr8:82951125-82951144 | MsG0880047173.01.T01:CDS | 55.0% | |
| ! | TGATAGAGCTCTGGCCGTGA+CGG | + | Chr8:82950955-82950974 | MsG0880047173.01.T01:CDS | 55.0% |
| CACGGCCAGAGCTCTATCAG+CGG | - | Chr8:82950954-82950973 | None:intergenic | 60.0% | |
| CGGCCAGAGGAAGAACTCGT+TGG | + | Chr8:82950975-82950994 | MsG0880047173.01.T01:CDS | 60.0% | |
| GCCGGGCCATTCTAATGACC+CGG | + | Chr8:82950999-82951018 | MsG0880047173.01.T01:CDS | 60.0% | |
| GCCGGGTCATTAGAATGGCC+CGG | - | Chr8:82951003-82951022 | None:intergenic | 60.0% | |
| TCTGCTTCTACTTCCGGCGC+CGG | + | Chr8:82951283-82951302 | MsG0880047173.01.T01:CDS | 60.0% | |
| ! | AACGGCTTTTCCTCGGCGCG+GGG | - | Chr8:82950924-82950943 | None:intergenic | 65.0% |
| ! | CGAACGGCTTTTCCTCGGCG+CGG | - | Chr8:82950926-82950945 | None:intergenic | 65.0% |
| ! | GAACGGCTTTTCCTCGGCGC+GGG | - | Chr8:82950925-82950944 | None:intergenic | 65.0% |
| TCATCATCCACCCCGCGCCG+AGG | + | Chr8:82950911-82950930 | MsG0880047173.01.T01:CDS | 70.0% | |
| ACCGGAACTGACGCCGGCGC+CGG | - | Chr8:82951299-82951318 | None:intergenic | 75.0% | |
| GCTCTGGCCGTGACGGCCAG+AGG | + | Chr8:82950962-82950981 | MsG0880047173.01.T01:CDS | 75.0% | |
| ! | GGCTTTTCCTCGGCGCGGGG+TGG | - | Chr8:82950921-82950940 | None:intergenic | 75.0% |
| !! | TCCGGCGCCGGCGTCAGTTC+CGG | + | Chr8:82951295-82951314 | MsG0880047173.01.T01:CDS | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr8 | gene | 82950713 | 82951321 | 82950713 | ID=MsG0880047173.01;Name=MsG0880047173.01 |
| Chr8 | mRNA | 82950713 | 82951321 | 82950713 | ID=MsG0880047173.01.T01;Parent=MsG0880047173.01;Name=MsG0880047173.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|202 |
| Chr8 | exon | 82950713 | 82951321 | 82950713 | ID=MsG0880047173.01.T01:exon:11033;Parent=MsG0880047173.01.T01 |
| Chr8 | CDS | 82950713 | 82951321 | 82950713 | ID=MsG0880047173.01.T01:cds;Parent=MsG0880047173.01.T01 |
| Gene Sequence |
| Protein sequence |
