AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0180004678.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180004678.01.T01 MTR_3g114960 61.538 130 16 1 74 203 1 96 7.40e-46 148
MsG0180004678.01.T01 MTR_2g055890 67.010 97 32 0 114 210 157 253 3.63e-39 139
MsG0180004678.01.T01 MTR_5g053570 63.918 97 35 0 114 210 329 425 2.72e-36 132
MsG0180004678.01.T01 MTR_0014s0220 65.979 97 33 0 114 210 250 346 4.95e-36 134
MsG0180004678.01.T01 MTR_6g055280 64.045 89 31 1 123 210 6 94 5.18e-33 116
MsG0180004678.01.T01 MTR_3g114950 79.452 73 13 1 1 71 5 77 1.93e-31 110
MsG0180004678.01.T01 MTR_3g114950 79.452 73 13 1 1 71 1 73 2.01e-31 110
MsG0180004678.01.T01 MTR_4g037500 63.014 73 23 1 138 210 113 181 7.00e-26 102
MsG0180004678.01.T01 MTR_4g088095 47.000 100 49 2 114 210 84 182 4.00e-25 97.1
MsG0180004678.01.T01 MTR_1g092960 46.000 100 50 2 114 210 18 116 5.88e-25 94.7
MsG0180004678.01.T01 MTR_3g102890 47.475 99 50 1 114 210 59 157 6.23e-25 96.3
MsG0180004678.01.T01 MTR_5g071700 48.485 99 49 1 114 210 27 125 5.04e-24 98.6
MsG0180004678.01.T01 MTR_0073s0010 46.000 100 52 1 113 210 92 191 9.56e-24 95.1
MsG0180004678.01.T01 MTR_5g032840 39.831 118 59 2 95 210 9 116 1.67e-23 97.1
MsG0180004678.01.T01 MTR_3g113230 45.000 100 53 1 113 210 107 206 2.05e-23 94.4
MsG0180004678.01.T01 MTR_7g022020 46.465 99 51 1 114 210 241 339 2.35e-22 94.7
MsG0180004678.01.T01 MTR_7g050355 46.000 100 50 2 114 210 269 367 2.62e-22 94.7
MsG0180004678.01.T01 MTR_3g048710 41.837 98 55 1 115 210 16 113 1.06e-21 87.4
MsG0180004678.01.T01 MTR_4g076760 40.708 113 61 2 104 210 114 226 2.60e-21 91.7
MsG0180004678.01.T01 MTR_1g021210 44.000 100 54 1 113 210 282 381 2.63e-21 91.7
MsG0180004678.01.T01 MTR_0631s0010 45.455 99 52 1 114 210 146 244 2.71e-21 89.0
MsG0180004678.01.T01 MTR_7g027013 47.475 99 50 2 114 210 389 487 2.76e-21 91.7
MsG0180004678.01.T01 MTR_3g109055 43.434 99 54 1 114 210 67 165 4.82e-21 87.4
MsG0180004678.01.T01 MTR_2g016390 41.593 113 60 2 104 210 93 205 8.02e-21 90.5
MsG0180004678.01.T01 MTR_4g086180 44.792 96 49 1 115 210 86 177 9.75e-21 89.7
MsG0180004678.01.T01 MTR_0003s0260 62.121 66 25 0 113 178 161 226 1.13e-20 86.7
MsG0180004678.01.T01 MTR_1g054110 41.237 97 55 1 116 210 69 165 3.33e-20 85.9
MsG0180004678.01.T01 MTR_0003s0410 45.833 96 48 1 115 210 188 279 3.93e-20 88.2
MsG0180004678.01.T01 MTR_0002s0870 44.565 92 49 1 121 210 61 152 5.51e-20 86.7
MsG0180004678.01.T01 MTR_8g071880 43.434 99 54 1 114 210 405 503 7.63e-20 87.4
MsG0180004678.01.T01 MTR_4g123460 45.652 92 47 2 117 206 110 200 8.48e-20 83.6
MsG0180004678.01.T01 MTR_1g057360 45.455 99 52 1 114 210 40 138 2.19e-19 85.1
MsG0180004678.01.T01 MTR_3g034020 41.176 102 54 2 113 210 74 173 2.31e-19 85.9
MsG0180004678.01.T01 MTR_6g024100 41.237 97 52 1 114 210 15 106 6.09e-19 82.4
MsG0180004678.01.T01 MTR_2g049690 47.126 87 40 2 128 210 92 176 1.46e-18 82.8
MsG0180004678.01.T01 MTR_1g012040 42.424 99 55 1 114 210 84 182 2.96e-18 82.8
MsG0180004678.01.T01 MTR_7g062790 42.857 84 46 1 128 209 86 169 5.86e-18 80.1
MsG0180004678.01.T01 MTR_7g034270 41.667 96 54 1 113 206 84 179 2.99e-17 79.3
MsG0180004678.01.T01 MTR_4g036720 45.455 77 42 0 113 189 92 168 9.27e-17 76.6
MsG0180004678.01.T01 MTR_2g089470 42.105 95 53 1 114 206 447 541 9.44e-17 78.6
MsG0180004678.01.T01 MTR_2g068990 42.105 95 53 1 114 206 447 541 9.44e-17 78.6
MsG0180004678.01.T01 MTR_5g040660 38.889 90 43 1 121 210 84 161 1.03e-16 75.1
MsG0180004678.01.T01 MTR_4g120080 43.158 95 52 1 114 206 442 536 2.38e-16 77.4
MsG0180004678.01.T01 MTR_8g469370 42.353 85 47 1 128 210 158 242 5.69e-16 75.9
MsG0180004678.01.T01 MTR_5g077050 50.725 69 34 0 114 182 114 182 1.28e-15 72.8
MsG0180004678.01.T01 MTR_1g033220 38.144 97 55 1 114 210 15 106 2.63e-15 73.2
MsG0180004678.01.T01 MTR_5g020370 36.735 98 44 2 114 209 105 186 2.93e-15 73.6
MsG0180004678.01.T01 MTR_2g066670 44.156 77 41 1 136 210 50 126 3.46e-15 73.6
MsG0180004678.01.T01 MTR_0356s0050 49.180 61 31 0 128 188 64 124 9.61e-15 70.5
MsG0180004678.01.T01 MTR_1g107470 35.652 115 51 4 100 210 46 141 1.38e-13 68.9
MsG0180004678.01.T01 MTR_7g031700 44.595 74 39 1 114 185 147 220 1.70e-13 68.6
MsG0180004678.01.T01 MTR_2g064230 37.000 100 40 2 113 210 42 120 3.48e-13 68.2
MsG0180004678.01.T01 MTR_5g054110 44.444 63 31 1 148 210 1 59 3.73e-13 65.1
MsG0180004678.01.T01 MTR_6g082730 43.077 65 37 0 121 185 84 148 3.77e-13 65.1
MsG0180004678.01.T01 MTR_7g070715 44.595 74 41 0 115 188 108 181 5.02e-13 67.4
MsG0180004678.01.T01 MTR_2g008505 43.243 74 42 0 115 188 108 181 1.86e-12 65.5
MsG0180004678.01.T01 MTR_0003s0390 35.000 100 52 2 113 210 132 220 2.61e-12 65.1
MsG0180004678.01.T01 MTR_6g029550 41.176 68 40 0 117 184 21 88 6.10e-11 61.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 33 sgRNAs with CRISPR-Local

Find 72 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
AATTAACCAAGTTGATAGTT+TGG 0.294275 1:-81133388 MsG0180004678.01.T01:CDS
ATTAACCAAGTTGATAGTTT+GGG 0.350971 1:-81133387 MsG0180004678.01.T01:CDS
AATTTAGGGTGATACGATTC+TGG 0.363554 1:-81134649 MsG0180004678.01.T01:intron
ATGACTCAAGTGTAGGTATT+GGG 0.372254 1:+81133456 None:intergenic
TAGCAGTAATGACGTGATTC+CGG 0.390263 1:+81133514 None:intergenic
GTCTTGACAGTTGCTATATC+CGG 0.412982 1:-81133533 MsG0180004678.01.T01:intron
TCAACATGCGATATAGATAA+AGG 0.413172 1:-81133434 MsG0180004678.01.T01:CDS
TTGTTCGTATTCTTGATGTA+TGG 0.421421 1:-81134810 MsG0180004678.01.T01:CDS
GAATGTATTGTTTGATCTCT+AGG 0.429691 1:+81133491 None:intergenic
GGGTAATGAACATCTTGAAA+TGG 0.456141 1:-81134770 MsG0180004678.01.T01:CDS
CCTTACTCATGCATTGATCA+AGG 0.459654 1:-81133710 MsG0180004678.01.T01:CDS
ACATCTTGAAATGGTTATGA+TGG 0.460858 1:-81134761 MsG0180004678.01.T01:CDS
TACAACCAAAACAAAATCTC+TGG 0.468366 1:-81133748 MsG0180004678.01.T01:CDS
AAACAAAACTTGTGAGCCAT+TGG 0.473349 1:+81133356 None:intergenic
AATGGTTATGATGGATTCAA+AGG 0.486827 1:-81134752 MsG0180004678.01.T01:intron
CAATGACTCTTAAGGAAACA+TGG 0.504014 1:-81134840 MsG0180004678.01.T01:CDS
CCTTGATCAATGCATGAGTA+AGG 0.504848 1:+81133710 None:intergenic
TTCATCCCAAACTATCAACT+TGG 0.517775 1:+81133382 None:intergenic
GATGACTCAAGTGTAGGTAT+TGG 0.534153 1:+81133455 None:intergenic
GTGGTGGTCACACATCTGAA+TGG 0.551131 1:-81134617 MsG0180004678.01.T01:CDS
TGGTCACACATCTGAATGGA+AGG 0.568427 1:-81134613 MsG0180004678.01.T01:CDS
GAATGGAAGGGAAAGATCAA+AGG 0.574476 1:-81134600 MsG0180004678.01.T01:CDS
CATGTTGATGACTCAAGTGT+AGG 0.584506 1:+81133449 None:intergenic
ATGGAGTGTTGTCAATAGCA+AGG 0.589997 1:-81134791 MsG0180004678.01.T01:CDS
AGTTTGGGATGAAGCTCCAA+TGG 0.596873 1:-81133372 MsG0180004678.01.T01:CDS
TGGAGTGTTGTCAATAGCAA+GGG 0.607318 1:-81134790 MsG0180004678.01.T01:CDS
ACACTCAAAGACATAATGAT+CGG 0.618640 1:-81133317 MsG0180004678.01.T01:CDS
ACGATTCTGGCTCTGATCCG+TGG 0.635981 1:-81134636 MsG0180004678.01.T01:CDS
ATGACGTGTATCATCAACAA+TGG 0.659985 1:-81134573 MsG0180004678.01.T01:CDS
GGTCACACATCTGAATGGAA+GGG 0.660205 1:-81134612 MsG0180004678.01.T01:CDS
ATTCTGGCTCTGATCCGTGG+TGG 0.693076 1:-81134633 MsG0180004678.01.T01:CDS
ATTCAGATGTGTGACCACCA+CGG 0.715063 1:+81134619 None:intergenic
GCATTGATCAAGGATGCACA+AGG 0.719197 1:-81133700 MsG0180004678.01.T01:intron

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATAATTTTCATTTAAAAACT+TGG + Chr1:81133833-81133852 None:intergenic 10.0%
!!! ATCAATATCAATTTTTTTTA+AGG - Chr1:81134074-81134093 MsG0180004678.01.T01:intron 10.0%
!!! CAACTATTTTTTTCAATTTA+GGG - Chr1:81133434-81133453 MsG0180004678.01.T01:CDS 15.0%
!!! TCAACTATTTTTTTCAATTT+AGG - Chr1:81133433-81133452 MsG0180004678.01.T01:CDS 15.0%
!! AAGTCTTCATCAAAAATATT+TGG - Chr1:81134807-81134826 MsG0180004678.01.T01:CDS 20.0%
!! AGTAGAAACTAAAATAAAAC+AGG + Chr1:81133891-81133910 None:intergenic 20.0%
!! TCAAAAAAATTTGTGTTTCT+TGG - Chr1:81133590-81133609 MsG0180004678.01.T01:intron 20.0%
!!! TATTATTATTAGTCTTAGCT+AGG - Chr1:81134434-81134453 MsG0180004678.01.T01:intron 20.0%
! AAAAAATTTGTGTTTCTTGG+TGG - Chr1:81133593-81133612 MsG0180004678.01.T01:intron 25.0%
! AATCAATTCATGACAAATGA+TGG + Chr1:81134221-81134240 None:intergenic 25.0%
! ACATTAATCAATGATGCAAA+TGG + Chr1:81134476-81134495 None:intergenic 25.0%
! ATACATTCATATATGAGTCT+TGG + Chr1:81133810-81133829 None:intergenic 25.0%
! ATCAATTCATGACAAATGAT+GGG + Chr1:81134220-81134239 None:intergenic 25.0%
! CAACTATTAACAGTTTCAAA+TGG - Chr1:81134502-81134521 MsG0180004678.01.T01:intron 25.0%
! CTTCATCAAAAATATTTGGA+GGG - Chr1:81134811-81134830 MsG0180004678.01.T01:CDS 25.0%
! TATTAACAGTTTCAAATGGT+TGG - Chr1:81134506-81134525 MsG0180004678.01.T01:intron 25.0%
! TCTTCATCAAAAATATTTGG+AGG - Chr1:81134810-81134829 MsG0180004678.01.T01:CDS 25.0%
!! ATATGATTAACTGCACTTTT+TGG - Chr1:81134296-81134315 MsG0180004678.01.T01:intron 25.0%
!! GCATCATTGATTAATGTTTA+TGG - Chr1:81134479-81134498 MsG0180004678.01.T01:intron 25.0%
!!! AATTAACCAAGTTGATAGTT+TGG - Chr1:81134709-81134728 MsG0180004678.01.T01:intron 25.0%
!!! ACAAAAGCGATACTTTTTTA+AGG - Chr1:81133652-81133671 MsG0180004678.01.T01:intron 25.0%
!!! ACAAGTTTTGTTTTGAAACA+TGG - Chr1:81134751-81134770 MsG0180004678.01.T01:intron 25.0%
!!! ATTAACCAAGTTGATAGTTT+GGG - Chr1:81134710-81134729 MsG0180004678.01.T01:intron 25.0%
!!! CAAGTTTTGTTTTGAAACAT+GGG - Chr1:81134752-81134771 MsG0180004678.01.T01:intron 25.0%
!!! TATGATTAACTGCACTTTTT+GGG - Chr1:81134297-81134316 MsG0180004678.01.T01:intron 25.0%
!!! TGTTTTGGTTGTAGTAATTT+AGG + Chr1:81134342-81134361 None:intergenic 25.0%
!!! TTAAAAAAGTATCGCTTTTG+TGG + Chr1:81133653-81133672 None:intergenic 25.0%
ACACTCAAAGACATAATGAT+CGG - Chr1:81134780-81134799 MsG0180004678.01.T01:CDS 30.0%
ACATCTTGAAATGGTTATGA+TGG - Chr1:81133336-81133355 MsG0180004678.01.T01:CDS 30.0%
GAATGTATTGTTTGATCTCT+AGG + Chr1:81134609-81134628 None:intergenic 30.0%
TACAACCAAAACAAAATCTC+TGG - Chr1:81134349-81134368 MsG0180004678.01.T01:intron 30.0%
TCAACATGCGATATAGATAA+AGG - Chr1:81134663-81134682 MsG0180004678.01.T01:intron 30.0%
TTGTTCGTATTCTTGATGTA+TGG - Chr1:81133287-81133306 MsG0180004678.01.T01:CDS 30.0%
TTTCAAAACTGTCTACATAG+TGG - Chr1:81134271-81134290 MsG0180004678.01.T01:intron 30.0%
! ATTTTGCTGAGATCCTTAAA+GGG - Chr1:81133675-81133694 MsG0180004678.01.T01:intron 30.0%
! GGAGGGAATGTAATTATTTT+CGG - Chr1:81134828-81134847 MsG0180004678.01.T01:CDS 30.0%
!! AATGGTTATGATGGATTCAA+AGG - Chr1:81133345-81133364 MsG0180004678.01.T01:CDS 30.0%
!! GCAAGTTGATATATGCTTTA+TGG - Chr1:81134192-81134211 MsG0180004678.01.T01:intron 30.0%
AAACAAAACTTGTGAGCCAT+TGG + Chr1:81134744-81134763 None:intergenic 35.0%
ACAGTTTCAAATGGTTGGAA+TGG - Chr1:81134511-81134530 MsG0180004678.01.T01:intron 35.0%
ATGACGTGTATCATCAACAA+TGG - Chr1:81133524-81133543 MsG0180004678.01.T01:CDS 35.0%
ATGACTCAAGTGTAGGTATT+GGG + Chr1:81134644-81134663 None:intergenic 35.0%
CAATGACTCTTAAGGAAACA+TGG - Chr1:81133257-81133276 MsG0180004678.01.T01:CDS 35.0%
GGGTAATGAACATCTTGAAA+TGG - Chr1:81133327-81133346 MsG0180004678.01.T01:CDS 35.0%
TTCATCCCAAACTATCAACT+TGG + Chr1:81134718-81134737 None:intergenic 35.0%
TTGACAGAGTCTTACAACAA+TGG + Chr1:81134028-81134047 None:intergenic 35.0%
! AATTTAGGGTGATACGATTC+TGG - Chr1:81133448-81133467 MsG0180004678.01.T01:CDS 35.0%
! GATTTTGCTGAGATCCTTAA+AGG - Chr1:81133674-81133693 MsG0180004678.01.T01:intron 35.0%
! GGGAATGTAATTATTTTCGG+TGG - Chr1:81134831-81134850 MsG0180004678.01.T01:CDS 35.0%
!! TAGCACCAGAGATTTTGTTT+TGG + Chr1:81134357-81134376 None:intergenic 35.0%
AAGGGAACTGCAAGTTAGAA+AGG - Chr1:81133693-81133712 MsG0180004678.01.T01:intron 40.0%
CCTTGATCAATGCATGAGTA+AGG + Chr1:81134390-81134409 None:intergenic 40.0%
GAATGGAAGGGAAAGATCAA+AGG - Chr1:81133497-81133516 MsG0180004678.01.T01:CDS 40.0%
GATGACTCAAGTGTAGGTAT+TGG + Chr1:81134645-81134664 None:intergenic 40.0%
GTCTTGACAGTTGCTATATC+CGG - Chr1:81134564-81134583 MsG0180004678.01.T01:intron 40.0%
GTGGTATGTCTTTCATCTCT+TGG + Chr1:81133634-81133653 None:intergenic 40.0%
TAGCAGTAATGACGTGATTC+CGG + Chr1:81134586-81134605 None:intergenic 40.0%
TGCAAGTTAGAAAGGCTTGA+AGG - Chr1:81133701-81133720 MsG0180004678.01.T01:CDS 40.0%
! CCTTACTCATGCATTGATCA+AGG - Chr1:81134387-81134406 MsG0180004678.01.T01:intron 40.0%
! CTAACTTGCAGTTCCCTTTA+AGG + Chr1:81133691-81133710 None:intergenic 40.0%
!! ATGGAGTGTTGTCAATAGCA+AGG - Chr1:81133306-81133325 MsG0180004678.01.T01:CDS 40.0%
!! CATGTTGATGACTCAAGTGT+AGG + Chr1:81134651-81134670 None:intergenic 40.0%
!! TGCACTTTTTGGGAAAGCTA+TGG - Chr1:81134307-81134326 MsG0180004678.01.T01:intron 40.0%
!! TGGAGTGTTGTCAATAGCAA+GGG - Chr1:81133307-81133326 MsG0180004678.01.T01:CDS 40.0%
ATTCAGATGTGTGACCACCA+CGG + Chr1:81133481-81133500 None:intergenic 45.0%
GGTCACACATCTGAATGGAA+GGG - Chr1:81133485-81133504 MsG0180004678.01.T01:CDS 45.0%
TGGTCACACATCTGAATGGA+AGG - Chr1:81133484-81133503 MsG0180004678.01.T01:CDS 45.0%
! AGTTTGGGATGAAGCTCCAA+TGG - Chr1:81134725-81134744 MsG0180004678.01.T01:intron 45.0%
! GCATTGATCAAGGATGCACA+AGG - Chr1:81134397-81134416 MsG0180004678.01.T01:intron 45.0%
GTGGTGGTCACACATCTGAA+TGG - Chr1:81133480-81133499 MsG0180004678.01.T01:CDS 50.0%
!! ACGATTCTGGCTCTGATCCG+TGG - Chr1:81133461-81133480 MsG0180004678.01.T01:CDS 55.0%
!! ATTCTGGCTCTGATCCGTGG+TGG - Chr1:81133464-81133483 MsG0180004678.01.T01:CDS 55.0%
Chromosome Type Strat End Strand Name
Chr1 gene 81133253 81134866 81133253 ID=MsG0180004678.01;Name=MsG0180004678.01
Chr1 mRNA 81133253 81134866 81133253 ID=MsG0180004678.01.T01;Parent=MsG0180004678.01;Name=MsG0180004678.01.T01;_AED=0.28;_eAED=0.28;_QI=0|0|0|0.75|1|0.75|4|0|210
Chr1 exon 81134753 81134866 81134753 ID=MsG0180004678.01.T01:exon:8338;Parent=MsG0180004678.01.T01
Chr1 exon 81134567 81134664 81134567 ID=MsG0180004678.01.T01:exon:8337;Parent=MsG0180004678.01.T01
Chr1 exon 81133701 81133828 81133701 ID=MsG0180004678.01.T01:exon:8336;Parent=MsG0180004678.01.T01
Chr1 exon 81133253 81133545 81133253 ID=MsG0180004678.01.T01:exon:8335;Parent=MsG0180004678.01.T01
Chr1 CDS 81134753 81134866 81134753 ID=MsG0180004678.01.T01:cds;Parent=MsG0180004678.01.T01
Chr1 CDS 81134567 81134664 81134567 ID=MsG0180004678.01.T01:cds;Parent=MsG0180004678.01.T01
Chr1 CDS 81133701 81133828 81133701 ID=MsG0180004678.01.T01:cds;Parent=MsG0180004678.01.T01
Chr1 CDS 81133253 81133545 81133253 ID=MsG0180004678.01.T01:cds;Parent=MsG0180004678.01.T01
Gene Sequence

>MsG0180004678.01.T01

ATGTCAATGACTCTTAAGGAAACATGGAAACTTGTTGTTCGTATTCTTGATGTATGGAGTGTTGTCAATAGCAAGGGTAATGAACATCTTGAAATGGTTATGATGGATTCAAAGGGTGATACGATTCTGGCTCTGATCCGTGGTGGTCACACATCTGAATGGAAGGGAAAGATCAAAGGAGATATGACGTGTATCATCAACAATGGTGTTGTTGGCAATATGATTAACTGCACTTTTTGGGAAAGCTATGGATCTAAGTTCCTAAATTACTACAACCAAAACAAAATCTCTGGTGCTATTGTCATTATCCTTACTCATGCATTGATCAAGGATGCACAAGTTGCTATATCCGGAATCACGTCATTACTGCTACCTAGAGATCAAACAATACATTCAAAGATTAAAATCCCAATACCTACACTTGAGTCATCAACATGCGATATAGATAAAGGAAGTGATCATACAGAACTTTTGAAATTAACCAAGTTGATAGTTTGGGATGAAGCTCCAATGGCTCACAAGTTTTGTTTTGAAACATGGGATAGAACACTCAAAGACATAATGATCGGATCTAAGTCTTCATCAAAAATATTTGGAGGGAATGTAATTATTTTCGGTGGTGATTTTCGATAA

Protein sequence

>MsG0180004678.01.T01

MSMTLKETWKLVVRILDVWSVVNSKGNEHLEMVMMDSKGDTILALIRGGHTSEWKGKIKGDMTCIINNGVVGNMINCTFWESYGSKFLNYYNQNKISGAIVIILTHALIKDAQVAISGITSLLLPRDQTIHSKIKIPIPTLESSTCDIDKGSDHTELLKLTKLIVWDEAPMAHKFCFETWDRTLKDIMIGSKSSSKIFGGNVIIFGGDFR*