AlfalfaGEDB | MsG0180004939.01

M. sativa cultivar ZhongmuNo.1 / MsG0180004939.01

KEGG: -
GO: -
PFAM: PF14868

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180004939.01.T01	ABE86678.1	94.524	347	17	1	137	481	748	1094	0	682
MsG0180004939.01.T01	ABE86678.1	93.478	46	3	0	78	123	117	162	1.32E-12	83.2

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180004939.01.T01	Q1RSI3	94.524	347	17	1	137	481	748	1094	0.0	682

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TFs/TRs:

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Gene ID	Type	Classification
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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0180004939.01	MsG0180004940.01	0.974343	5.471117e-138	7.559788e-132
MsG0180004939.01	MsG0680034392.01	0.804702	1.902883e-49	9.878359e-47

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PPI

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Gene1	Gene2	Type
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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180004939.01.T01	MTR_1g090050	98.750	320	4	0	162	481	681	1000	0.0	666
MsG0180004939.01.T01	MTR_1g090050	94.000	50	3	0	74	123	102	151	1.31e-18	89.7

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180004939.01.T01	AT1G04650	41.479	311	161	5	162	469	603	895	2.58e-65	226
MsG0180004939.01.T01	AT1G04650	41.479	311	161	5	162	469	705	997	4.82e-65	227

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Find 76 sgRNAs with CRISPR-Local

Find 396 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAATGGCGGAAGGAAACTC+CGG	0.586569	1:+84704450	None:intergenic
AAATAAAGGACCATTCTCTT+CGG	0.505358	1:+84710893	MsG0180004940.01.T01:CDS
AACATAAATATAAATGATTC+AGG	0.336646	1:-84708187	None:intergenic
AAGCTGATGCTGAAAATCTT+AGG	0.334216	1:-84708229	None:intergenic
AATATCGGTGAATTTCAACA+AGG	0.727967	1:-84708258	None:intergenic
AATCAGTGAACAGAATGAAA+AGG	0.523161	1:-84704560	None:intergenic
AATTACTCACTTCGAGGAAT+TGG	0.343356	1:+84711695	MsG0180004940.01.T01:CDS
ACACAACTTGCTGTCAGCTA+TGG	0.527197	1:+84711217	MsG0180004940.01.T01:CDS
ACATTTCAGTCAATGCTGAA+AGG	0.623736	1:+84711430	MsG0180004940.01.T01:CDS
ACTACCTGAGATTGATGCAA+GGG	0.602615	1:+84710816	MsG0180004940.01.T01:CDS

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAAACTTACATTTGACTCTG+AGG	-	Chr1:84705232-84705251	None:intergenic	30.0%
AAAATGAAAGCAAAACGAAG+TGG	-	Chr1:84704607-84704626	None:intergenic	30.0%
AAAATGGGAACACTATTCTA+AGG	-	Chr1:84710781-84710800	None:intergenic	30.0%
AAAGAAGTTCTCTAGTAAGA+AGG	-	Chr1:84709970-84709989	None:intergenic	30.0%
AAATAAAGGACCATTCTCTT+CGG	+	Chr1:84710893-84710912	MsG0180004940.01.T01:CDS	30.0%
AAGACAATCAGCAAATCAAT+AGG	-	Chr1:84707620-84707639	None:intergenic	30.0%
AAGATATCATATACCTTTGC+TGG	+	Chr1:84706844-84706863	MsG0180004940.01.T01:intron	30.0%
AATATCGGTGAATTTCAACA+AGG	-	Chr1:84708261-84708280	None:intergenic	30.0%
AATCAGTGAACAGAATGAAA+AGG	-	Chr1:84704563-84704582	None:intergenic	30.0%
AATGATTTATCAGTCCTTTG+AGG	+	Chr1:84708628-84708647	MsG0180004940.01.T01:intron	30.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr1	gene	84704453	84711770	84704453	ID=MsG0180004939.01;Name=MsG0180004939.01
Chr1	mRNA	84704453	84711770	84704453	ID=MsG0180004939.01.T01;Parent=MsG0180004939.01;Name=MsG0180004939.01.T01;_AED=0.39;_eAED=0.42;_QI=0\|0\|0\|1\|0\|0.25\|4\|0\|481
Chr1	exon	84704453	84704586	84704453	ID=MsG0180004939.01.T01:exon:9376;Parent=MsG0180004939.01.T01
Chr1	exon	84708136	84708383	84708136	ID=MsG0180004939.01.T01:exon:9377;Parent=MsG0180004939.01.T01
Chr1	exon	84709800	84709876	84709800	ID=MsG0180004939.01.T01:exon:9378;Parent=MsG0180004939.01.T01
Chr1	exon	84710784	84711770	84710784	ID=MsG0180004939.01.T01:exon:9379;Parent=MsG0180004939.01.T01
Chr1	CDS	84704453	84704586	84704453	ID=MsG0180004939.01.T01:cds;Parent=MsG0180004939.01.T01
Chr1	CDS	84708136	84708383	84708136	ID=MsG0180004939.01.T01:cds;Parent=MsG0180004939.01.T01
Chr1	CDS	84709800	84709876	84709800	ID=MsG0180004939.01.T01:cds;Parent=MsG0180004939.01.T01
Chr1	CDS	84710784	84711770	84710784	ID=MsG0180004939.01.T01:cds;Parent=MsG0180004939.01.T01

Gene Sequence

>MsG0180004939.01.T01

ATGGCGGAAGGAAACTCCGGCGCCGATATCCACCGTATACTCGCCGCCGTTAAATCCTCCGAGGCGAGCTACTTCCATTTCTCGCTTCAATTTTTACTCGTCAACGACCTTTTCATTCTGTTCACTGATTTAGGGAATCTGCGTGTTTGTTGGGCTAACATTGATTTTAGTGACATCATATTTTACCTGAATCATTTATATTTATGTTCTGCAGCTTCTTTGGAGATCCTAAGATTTTCAGCATCAGCTTTTTCGGCCTTGTTGAAATTCACCGATATTAGTAACAAGGAATTGATGGACAATGTTGAAATTTTCACTATAGAAGTGTTAAATCTAACTAAAGATTCTATATCTGAAGCCAAGGTAATATTTTTCTTCCTTTTTGAGGAAGATGTATATTCTTGTATGCTTGTTTTGCAACTTCCATCATTATCTGGCTCTGGAAAAACAGCTGAACTGAATAGTGTTCCCATTTTATGCAGCAAGGAAAGACTACCTGAGATTGATGCAAGGGCCATAAAGTTTTTGGTTTCAATTAGTTCTAACTACAAAAGTACTGTTGACAAAGAAATAAAGGACCATTCTCTTCGGCTTTTTGGTGAAATTCTAGTGATCATTTCATATCTGGAACATCTATACAAATCGAATGATATTGAACAGGCCATAATACAGATTGAAAATATCTTTATCACTGAGCCTCCAGTCCTTTTGTACAAATGCAAACCACATTTGGCTCAGTTCCTCACTGGACTTGTTCACATGGAATTTTCAGAAAGTGATGATGGTGATGCAAAAAGTCGTGCTGCATGGGAATTATTTCACTTGATCTTGACAGAGCGGCACTGGGCATTCACACATTTAGCATTAACTGCATTTGGATATTTTGCTGCTAACACAACTTGCTGTCAGCTATGGAGATTTCTTCCACCGGATGCAGCACTTTCATATGATATAATTTCAGGAACAGAGACAACTAAGGAGAGATTTTATGCTGAGCTTAGAGAATTTCTTAATAAGCAAAATGCTCTTCTAACAGTTGCACCAAGTCCTGAACAGCTTGAGCTGCTACGACGAGAAGGTTTTATGCTAAAGCAGATGATCCATAACATTTCAGTCAATGCTGAAAGGAGAGATGGATGTGAGAGCATGGAAATTGATGACAAGAACCAATTAAATAGCATGGAAGTCGATGACATGAGCCAAACAAATAAAAAAAGGAAACTTCCAGCAGGAATTAGCAAAGGAGTGGAATTGCTGAAAAACGGTTTGAAGATTATTGGTGACGGTCTTTCAGAGTGGCAGCTTAATCAGTTTGAAACCAATGAACTGCATGTCAAGTATTCTGCTCAGTTTTCTCGACTTGAAGATGCAATTACTCACTTCGAGGAATTGGCTGGGAGTGGCGAGGTATGTTTATCTCCTATTCAGAGCAATTTGCGAGGTTGA

Protein sequence

>MsG0180004939.01.T01

MAEGNSGADIHRILAAVKSSEASYFHFSLQFLLVNDLFILFTDLGNLRVCWANIDFSDIIFYLNHLYLCSAASLEILRFSASAFSALLKFTDISNKELMDNVEIFTIEVLNLTKDSISEAKVIFFFLFEEDVYSCMLVLQLPSLSGSGKTAELNSVPILCSKERLPEIDARAIKFLVSISSNYKSTVDKEIKDHSLRLFGEILVIISYLEHLYKSNDIEQAIIQIENIFITEPPVLLYKCKPHLAQFLTGLVHMEFSESDDGDAKSRAAWELFHLILTERHWAFTHLALTAFGYFAANTTCCQLWRFLPPDAALSYDIISGTETTKERFYAELREFLNKQNALLTVAPSPEQLELLRREGFMLKQMIHNISVNAERRDGCESMEIDDKNQLNSMEVDDMSQTNKKRKLPAGISKGVELLKNGLKIIGDGLSEWQLNQFETNELHVKYSAQFSRLEDAITHFEELAGSGEVCLSPIQSNLRG*