AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0180005035.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180005035.01.T01 MTR_1g492730 96.484 256 9 0 1 256 1 256 0.0 515
MsG0180005035.01.T01 MTR_1g492730 95.714 210 9 0 1 210 1 210 3.98e-143 400
MsG0180005035.01.T01 MTR_1g052405 45.963 161 67 3 102 256 135 281 1.11e-42 148
MsG0180005035.01.T01 MTR_3g085620 56.667 90 39 0 167 256 40 129 8.50e-36 125
MsG0180005035.01.T01 MTR_1g052380 53.535 99 45 1 159 256 50 148 2.30e-32 118
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180005035.01.T01 AT3G57810 64.894 94 33 0 163 256 166 259 1.17e-40 142
MsG0180005035.01.T01 AT3G57810 64.894 94 33 0 163 256 166 259 1.17e-40 142
MsG0180005035.01.T01 AT3G57810 64.894 94 33 0 163 256 166 259 1.17e-40 142
MsG0180005035.01.T01 AT3G57810 39.362 94 52 2 123 211 121 214 9.55e-15 71.6

Find 106 sgRNAs with CRISPR-Local

Find 142 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GGGAACTTGCTGACGAATTA+AGG 0.193803 1:+86014788 MsG0180005035.01.T01:CDS
CTTAATAATAAAACCCGTTA+TGG 0.284416 1:+86013483 MsG0180005035.01.T01:five_prime_UTR
CTCCACCGCCGGTTGAGATT+TGG 0.298384 1:-86013721 None:intergenic
AACATAAGTCTGTTGAATTC+TGG 0.316175 1:-86015104 None:intergenic
TGGAATGGTGGCGGCGGGCG+GGG 0.325826 1:-86013682 None:intergenic
TGAGATTTGGAGGTGGTTGC+AGG 0.327466 1:-86013708 None:intergenic
GGTGGAATGGTGGCGGCGGG+CGG 0.330689 1:-86013684 None:intergenic
AATTCAACAGACTTATGTTT+GGG 0.341402 1:+86015108 MsG0180005035.01.T01:CDS
AGGAGAGCCTGAGCTGTTAA+TGG 0.343536 1:+86015132 MsG0180005035.01.T01:CDS
GTGGAATGGTGGCGGCGGGC+GGG 0.352247 1:-86013683 None:intergenic
GCGCCACTCGCTCTCGTCCT+TGG 0.358149 1:+86013591 MsG0180005035.01.T01:CDS
TTGGTTCCATTTCAAAGAAA+AGG 0.362070 1:+86013341 None:intergenic
AGAGAAGCGTGGGTGTTATA+AGG 0.369781 1:-86013375 None:intergenic
CTGCGCTTGCCTTGCGCCGC+CGG 0.373717 1:+86013919 MsG0180005035.01.T01:CDS
GAAAGGCGTGGTGGTGGTGG+TGG 0.384833 1:-86013625 None:intergenic
TGGAGTCGGCGGCGCCGCCT+CGG 0.392242 1:-86013960 None:intergenic
GAATTCAACAGACTTATGTT+TGG 0.396680 1:+86015107 MsG0180005035.01.T01:CDS
ACAAGTTAAACCGTTGTTGT+AGG 0.396916 1:-86015316 None:intergenic
GGCGGTGGAATGGTGGCGGC+GGG 0.418826 1:-86013687 None:intergenic
GGAACTTGCTGACGAATTAA+GGG 0.420114 1:+86014789 MsG0180005035.01.T01:CDS
ATCTTTCACTTCACGTCCCT+CGG 0.423234 1:-86014005 None:intergenic
GAAGCATCACACCGCTGCTT+CGG 0.424353 1:-86013790 None:intergenic
TCCCTCTGTCGACTTTCATT+AGG 0.424371 1:-86014769 None:intergenic
CTCTCGTCCGGGAAAACAGC+TGG 0.428780 1:-86013980 None:intergenic
TTGGAGGTGGTTGCAGGCGG+TGG 0.429035 1:-86013702 None:intergenic
CACTGTGTATGTGAGTTGTA+TGG 0.436105 1:-86013528 None:intergenic
ATTACTCCTTGCGGCGGCGA+TGG 0.436846 1:+86013764 MsG0180005035.01.T01:CDS
CGTCCCTCGGAACTCTCGTC+CGG 0.439061 1:-86013992 None:intergenic
TTATCCTACGCACCCATAAC+GGG 0.440177 1:-86013496 None:intergenic
GAGTTCATCGTCACGTTCAT+CGG 0.445917 1:-86014035 None:intergenic
AAGGATCGTGGAACGTTGCT+TGG 0.447140 1:+86013837 MsG0180005035.01.T01:CDS
TTCATCGTTCTGACTCAGCT+TGG 0.448002 1:+86013882 MsG0180005035.01.T01:CDS
TTAATAATAAAACCCGTTAT+GGG 0.452990 1:+86013484 MsG0180005035.01.T01:five_prime_UTR
TGGTTCCATTTCAAAGAAAA+GGG 0.456152 1:+86013342 None:intergenic
TGTGTCTATAGGTTTATAGA+AGG 0.457572 1:+86015061 MsG0180005035.01.T01:intron
GCGGTGGAGCTGCTTCGATA+TGG 0.467969 1:+86013735 MsG0180005035.01.T01:CDS
GACGAAAGGCGTGGTGGTGG+TGG 0.470943 1:-86013628 None:intergenic
AGGCGGTGGAATGGTGGCGG+CGG 0.476240 1:-86013688 None:intergenic
CACCGCCGGTTGAGATTTGG+AGG 0.479044 1:-86013718 None:intergenic
GTATCAACGTCAACAATAAC+CGG 0.479760 1:-86013938 None:intergenic
CTTGGGATGCTCGTCCTGCT+AGG 0.480235 1:+86013855 MsG0180005035.01.T01:CDS
AAAGGCGTGGTGGTGGTGGT+GGG 0.493789 1:-86013624 None:intergenic
CTCCAAATCTCAACCGGCGG+TGG 0.496363 1:+86013719 MsG0180005035.01.T01:CDS
GGTGGTTGCAGGCGGTGGAA+TGG 0.499927 1:-86013697 None:intergenic
GCCACTCACTCATCCATGCT+TGG 0.503058 1:+86013560 MsG0180005035.01.T01:exon
ATCCTTCACCTTTGAGCTCG+TGG 0.505031 1:-86013820 None:intergenic
AGAGAAGAGAGCAATAAGAA+TGG 0.511242 1:-86013424 None:intergenic
CGCCGGTTGAGATTTGGAGG+TGG 0.513387 1:-86013715 None:intergenic
AACCACCTCCAAATCTCAAC+CGG 0.518771 1:+86013713 MsG0180005035.01.T01:CDS
GTTATCCTACGCACCCATAA+CGG 0.524419 1:-86013497 None:intergenic
AGGATCGTGGAACGTTGCTT+GGG 0.525778 1:+86013838 MsG0180005035.01.T01:CDS
TGCAGGCGGTGGAATGGTGG+CGG 0.528482 1:-86013691 None:intergenic
ACTGTGTATGTGAGTTGTAT+GGG 0.532059 1:-86013527 None:intergenic
AACAACGGTTTAACTTGTTG+TGG 0.533614 1:+86015321 MsG0180005035.01.T01:three_prime_UTR
CGGCGGGCGGGGAAAGTGAG+AGG 0.534229 1:-86013671 None:intergenic
TGAGAAGCCATTAACAGCTC+AGG 0.536531 1:-86015139 None:intergenic
GTCCCTCGGAACTCTCGTCC+GGG 0.539858 1:-86013991 None:intergenic
CTGTTCAGAGCAATAGCTCA+TGG 0.539965 1:+86014718 MsG0180005035.01.T01:CDS
GTGTCTATAGGTTTATAGAA+GGG 0.543445 1:+86015062 MsG0180005035.01.T01:intron
TGTCTATAGGTTTATAGAAG+GGG 0.544260 1:+86015063 MsG0180005035.01.T01:intron
GAACGATGAAGCCACCTAGC+AGG 0.545883 1:-86013869 None:intergenic
GGTTGCAGGCGGTGGAATGG+TGG 0.547561 1:-86013694 None:intergenic
TAGGGGTGCTTGCTGATGGT+CGG 0.550798 1:+86014692 MsG0180005035.01.T01:intron
CAACAATAACCGGCGGCGCA+AGG 0.554275 1:-86013928 None:intergenic
CGGAATCCATCGCCGCCGCA+AGG 0.559044 1:-86013770 None:intergenic
GGCGCAGAGTACACCAAGCA+TGG 0.563817 1:-86013573 None:intergenic
AGCTCAAAGGTGAAGGATCG+TGG 0.569548 1:+86013825 MsG0180005035.01.T01:CDS
AACGCTGATTACAGAGTCAC+GGG 0.570421 1:+86014058 MsG0180005035.01.T01:CDS
GAAGAGAAAGAGAGAAGCGT+GGG 0.573463 1:-86013385 None:intergenic
TCCGGGAAAACAGCTGGAGT+CGG 0.574081 1:-86013974 None:intergenic
CAACAGACTTATGTTTGGGG+AGG 0.576052 1:+86015112 MsG0180005035.01.T01:CDS
GACCACGAGCTCAAAGGTGA+AGG 0.577751 1:+86013818 MsG0180005035.01.T01:CDS
AAAACCCGTTATGGGTGCGT+AGG 0.577803 1:+86013492 MsG0180005035.01.T01:five_prime_UTR
GATCCAAGGACGAGAGCGAG+TGG 0.579496 1:-86013594 None:intergenic
GGTATAGGGGTGCTTGCTGA+TGG 0.584649 1:+86014688 MsG0180005035.01.T01:intron
TATCGAAGCAGCTCCACCGC+CGG 0.586713 1:-86013732 None:intergenic
CATCATGACCACGAGCTCAA+AGG 0.587256 1:+86013812 MsG0180005035.01.T01:CDS
TTCCCGGACGAGAGTTCCGA+GGG 0.588950 1:+86013989 MsG0180005035.01.T01:CDS
AGAGACGAAAGGCGTGGTGG+TGG 0.589008 1:-86013631 None:intergenic
CCATTTCAAAGAAAAGGGTG+AGG 0.599948 1:+86013347 None:intergenic
GAAAGTGAGAGGAGCGACAG+TGG 0.606183 1:-86013660 None:intergenic
CAACGCTGATTACAGAGTCA+CGG 0.607136 1:+86014057 MsG0180005035.01.T01:CDS
CACGCGATTACTCCTTGCGG+CGG 0.611520 1:+86013758 MsG0180005035.01.T01:CDS
TTTCCCGGACGAGAGTTCCG+AGG 0.612131 1:+86013988 MsG0180005035.01.T01:CDS
GCCTAATGAAAGTCGACAGA+GGG 0.612158 1:+86014768 MsG0180005035.01.T01:CDS
AACATCATATCCTACAACAA+CGG 0.619427 1:+86015306 MsG0180005035.01.T01:three_prime_UTR
CGAAGAGAAAGAGAGAAGCG+TGG 0.621048 1:-86013386 None:intergenic
GGCGATGGATTCCGAAGCAG+CGG 0.621773 1:+86013779 MsG0180005035.01.T01:CDS
TATTGTTGACGTTGATACCG+AGG 0.628764 1:+86013943 MsG0180005035.01.T01:CDS
GGGAAAACAGCTGGAGTCGG+CGG 0.628808 1:-86013971 None:intergenic
ACCAAGCATGGATGAGTGAG+TGG 0.631102 1:-86013561 None:intergenic
GATTTGGAGGTGGTTGCAGG+CGG 0.637060 1:-86013705 None:intergenic
TGGTGGGAAGAGAAGATCCA+AGG 0.650314 1:-86013608 None:intergenic
CGCCTAATGAAAGTCGACAG+AGG 0.650904 1:+86014767 MsG0180005035.01.T01:CDS
CACCTCCAAATCTCAACCGG+CGG 0.653859 1:+86013716 MsG0180005035.01.T01:CDS
GGGATGCTCGTCCTGCTAGG+TGG 0.655002 1:+86013858 MsG0180005035.01.T01:CDS
CTGTGTATGTGAGTTGTATG+GGG 0.657680 1:-86013526 None:intergenic
TCAAAGAAAAGGGTGAGGTG+AGG 0.658299 1:+86013352 None:intergenic
ACAGGCGCAAGGAAACAGAA+TGG 0.661069 1:+86014950 MsG0180005035.01.T01:CDS
ATTCAACAGACTTATGTTTG+GGG 0.663997 1:+86015109 MsG0180005035.01.T01:CDS
GAGAGAGACGAAAGGCGTGG+TGG 0.678901 1:-86013634 None:intergenic
CGTGAGAGAGACGAAAGGCG+TGG 0.686067 1:-86013637 None:intergenic
AGTGGCGTGAGAGAGACGAA+AGG 0.697149 1:-86013642 None:intergenic
TGGCACGCGATTACTCCTTG+CGG 0.725561 1:+86013755 MsG0180005035.01.T01:CDS
TGTTGACGTTGATACCGAGG+CGG 0.731095 1:+86013946 MsG0180005035.01.T01:CDS
TCAACGTCAACAATAACCGG+CGG 0.746562 1:-86013935 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTATTTTACTTTTATTTACA+TGG + Chr1:86015017-86015036 MsG0180005035.01.T01:intron 10.0%
!! AAATAAAACATCACAAAAAT+TGG - Chr1:86014909-86014928 None:intergenic 15.0%
!! AATAAAACATCACAAAAATT+GGG - Chr1:86014908-86014927 None:intergenic 15.0%
!!! ATATTTTTTCATATAAGTAC+AGG + Chr1:86014261-86014280 MsG0180005035.01.T01:intron 15.0%
!!! ATTTCATTTTTTGTCTTTAA+TGG + Chr1:86014238-86014257 MsG0180005035.01.T01:intron 15.0%
!!! ATTTTTGTGATGTTTTATTT+AGG + Chr1:86014909-86014928 MsG0180005035.01.T01:intron 15.0%
!!! TATTTTTTCATATAAGTACA+GGG + Chr1:86014262-86014281 MsG0180005035.01.T01:intron 15.0%
!! TTAATAATAAAACCCGTTAT+GGG + Chr1:86013484-86013503 MsG0180005035.01.T01:five_prime_UTR 20.0%
!!! CTTTTATTTACATGGTAAAT+TGG + Chr1:86015025-86015044 MsG0180005035.01.T01:intron 20.0%
!!! TTATTGTTCAGTTATTTTGA+TGG + Chr1:86014596-86014615 MsG0180005035.01.T01:intron 20.0%
! AACAAGAACTATTGTATCAT+TGG - Chr1:86014510-86014529 None:intergenic 25.0%
! AATTCAACAGACTTATGTTT+GGG + Chr1:86015108-86015127 MsG0180005035.01.T01:CDS 25.0%
! CTTAATAATAAAACCCGTTA+TGG + Chr1:86013483-86013502 MsG0180005035.01.T01:five_prime_UTR 25.0%
! GGAAACTACATACAATTATT+TGG + Chr1:86014991-86015010 MsG0180005035.01.T01:intron 25.0%
! TATAAGTTTGTATCACTGAA+TGG + Chr1:86014667-86014686 MsG0180005035.01.T01:intron 25.0%
!!! TTCTCTTCGTTCTTTTTTAT+TGG + Chr1:86013400-86013419 MsG0180005035.01.T01:five_prime_UTR 25.0%
AACATAAGTCTGTTGAATTC+TGG - Chr1:86015107-86015126 None:intergenic 30.0%
AACATCATATCCTACAACAA+CGG + Chr1:86015306-86015325 MsG0180005035.01.T01:three_prime_UTR 30.0%
AACATGAGTTAGAGATTATG+TGG + Chr1:86014637-86014656 MsG0180005035.01.T01:intron 30.0%
ACATGAGTTAGAGATTATGT+GGG + Chr1:86014638-86014657 MsG0180005035.01.T01:intron 30.0%
ACTTGTTCAATATGAAGTGT+CGG + Chr1:86014547-86014566 MsG0180005035.01.T01:intron 30.0%
ATTCAACAGACTTATGTTTG+GGG + Chr1:86015109-86015128 MsG0180005035.01.T01:CDS 30.0%
GAATTCAACAGACTTATGTT+TGG + Chr1:86015107-86015126 MsG0180005035.01.T01:CDS 30.0%
GTGTCTATAGGTTTATAGAA+GGG + Chr1:86015062-86015081 MsG0180005035.01.T01:intron 30.0%
TGGGTAACAAAATTCATTGA+AGG - Chr1:86014889-86014908 None:intergenic 30.0%
TGTCTATAGGTTTATAGAAG+GGG + Chr1:86015063-86015082 MsG0180005035.01.T01:intron 30.0%
TGTGTCTATAGGTTTATAGA+AGG + Chr1:86015061-86015080 MsG0180005035.01.T01:intron 30.0%
TTACAACTACGAAACAACAA+CGG + Chr1:86014425-86014444 MsG0180005035.01.T01:intron 30.0%
TTGTATCACTGAATGGTATA+GGG + Chr1:86014674-86014693 MsG0180005035.01.T01:intron 30.0%
TTTCATATAAGTACAGGGAT+TGG + Chr1:86014267-86014286 MsG0180005035.01.T01:intron 30.0%
TTTGTATCACTGAATGGTAT+AGG + Chr1:86014673-86014692 MsG0180005035.01.T01:intron 30.0%
!! GTTATATTTGCTGTGTCTAT+AGG + Chr1:86015050-86015069 MsG0180005035.01.T01:intron 30.0%
!! TCTTGCTAGAACTTTTGAAA+CGG + Chr1:86014826-86014845 MsG0180005035.01.T01:intron 30.0%
!!! ACGGGTTTTATTATTAAGAC+TGG - Chr1:86013481-86013500 None:intergenic 30.0%
ACAAGTTAAACCGTTGTTGT+AGG - Chr1:86015319-86015338 None:intergenic 35.0%
ACTGTGTATGTGAGTTGTAT+GGG - Chr1:86013530-86013549 None:intergenic 35.0%
AGAGAAGAGAGCAATAAGAA+TGG - Chr1:86013427-86013446 None:intergenic 35.0%
ATTGAACAAGTGTCTTGAGT+TGG - Chr1:86014538-86014557 None:intergenic 35.0%
GTATCAACGTCAACAATAAC+CGG - Chr1:86013941-86013960 None:intergenic 35.0%
TGTATCACTGAATGGTATAG+GGG + Chr1:86014675-86014694 MsG0180005035.01.T01:intron 35.0%
! ATATAAGTACAGGGATTGGT+TGG + Chr1:86014271-86014290 MsG0180005035.01.T01:intron 35.0%
! TTGCTGAAGAACTTTTGAAC+AGG + Chr1:86014932-86014951 MsG0180005035.01.T01:CDS 35.0%
!! TGGTGAGCTTTGTTATTTGA+AGG + Chr1:86014970-86014989 MsG0180005035.01.T01:intron 35.0%
CACTGTGTATGTGAGTTGTA+TGG - Chr1:86013531-86013550 None:intergenic 40.0%
CTGTGTATGTGAGTTGTATG+GGG - Chr1:86013529-86013548 None:intergenic 40.0%
GTGACAAACCATCAAACACT+TGG - Chr1:86014396-86014415 None:intergenic 40.0%
GTTTGTGACCAAGTGTTTGA+TGG + Chr1:86014385-86014404 MsG0180005035.01.T01:intron 40.0%
TATTGTTGACGTTGATACCG+AGG + Chr1:86013943-86013962 MsG0180005035.01.T01:CDS 40.0%
! AGCTTGCTTGAAAAATGGAG+AGG + Chr1:86014741-86014760 MsG0180005035.01.T01:CDS 40.0%
! CATGGAGCTTGCTTGAAAAA+TGG + Chr1:86014736-86014755 MsG0180005035.01.T01:CDS 40.0%
!! GGAACTTGCTGACGAATTAA+GGG + Chr1:86014789-86014808 MsG0180005035.01.T01:CDS 40.0%
!!! CTAGAACTTTTGAAACGGAG+AGG + Chr1:86014831-86014850 MsG0180005035.01.T01:intron 40.0%
AACCACCTCCAAATCTCAAC+CGG + Chr1:86013713-86013732 MsG0180005035.01.T01:CDS 45.0%
ATCTTTCACTTCACGTCCCT+CGG - Chr1:86014008-86014027 None:intergenic 45.0%
CAACAGACTTATGTTTGGGG+AGG + Chr1:86015112-86015131 MsG0180005035.01.T01:CDS 45.0%
CTGTTCAGAGCAATAGCTCA+TGG + Chr1:86014718-86014737 MsG0180005035.01.T01:CDS 45.0%
GAGTTCATCGTCACGTTCAT+CGG - Chr1:86014038-86014057 None:intergenic 45.0%
GCCTAATGAAAGTCGACAGA+GGG + Chr1:86014768-86014787 MsG0180005035.01.T01:CDS 45.0%
GTTATCCTACGCACCCATAA+CGG - Chr1:86013500-86013519 None:intergenic 45.0%
TCAACGTCAACAATAACCGG+CGG - Chr1:86013938-86013957 None:intergenic 45.0%
TGAGAAGCCATTAACAGCTC+AGG - Chr1:86015142-86015161 None:intergenic 45.0%
TTATCCTACGCACCCATAAC+GGG - Chr1:86013499-86013518 None:intergenic 45.0%
TTCATCGTTCTGACTCAGCT+TGG + Chr1:86013882-86013901 MsG0180005035.01.T01:CDS 45.0%
! AACGCTGATTACAGAGTCAC+GGG + Chr1:86014058-86014077 MsG0180005035.01.T01:CDS 45.0%
! AGAACTTTTGAACAGGCGCA+AGG + Chr1:86014939-86014958 MsG0180005035.01.T01:CDS 45.0%
! AGAGAAGCGTGGGTGTTATA+AGG - Chr1:86013378-86013397 None:intergenic 45.0%
! CAACGCTGATTACAGAGTCA+CGG + Chr1:86014057-86014076 MsG0180005035.01.T01:CDS 45.0%
! GAAGAGAAAGAGAGAAGCGT+GGG - Chr1:86013388-86013407 None:intergenic 45.0%
! GACTCAGCTTGGCTTCTTTT+CGG + Chr1:86013893-86013912 MsG0180005035.01.T01:CDS 45.0%
!! GGGAACTTGCTGACGAATTA+AGG + Chr1:86014788-86014807 MsG0180005035.01.T01:CDS 45.0%
!! TAAGTACAGGGATTGGTTGG+TGG + Chr1:86014274-86014293 MsG0180005035.01.T01:intron 45.0%
!! TCCCTCTGTCGACTTTCATT+AGG - Chr1:86014772-86014791 None:intergenic 45.0%
AAAACCCGTTATGGGTGCGT+AGG + Chr1:86013492-86013511 MsG0180005035.01.T01:five_prime_UTR 50.0%
AAGGATCGTGGAACGTTGCT+TGG + Chr1:86013837-86013856 MsG0180005035.01.T01:CDS 50.0%
ACAGGCGCAAGGAAACAGAA+TGG + Chr1:86014950-86014969 MsG0180005035.01.T01:CDS 50.0%
ACCAAGCATGGATGAGTGAG+TGG - Chr1:86013564-86013583 None:intergenic 50.0%
AGCTCAAAGGTGAAGGATCG+TGG + Chr1:86013825-86013844 MsG0180005035.01.T01:CDS 50.0%
AGGAGAGCCTGAGCTGTTAA+TGG + Chr1:86015132-86015151 MsG0180005035.01.T01:CDS 50.0%
AGGATCGTGGAACGTTGCTT+GGG + Chr1:86013838-86013857 MsG0180005035.01.T01:CDS 50.0%
ATCCTTCACCTTTGAGCTCG+TGG - Chr1:86013823-86013842 None:intergenic 50.0%
CATCATGACCACGAGCTCAA+AGG + Chr1:86013812-86013831 MsG0180005035.01.T01:CDS 50.0%
CGAAGAGAAAGAGAGAAGCG+TGG - Chr1:86013389-86013408 None:intergenic 50.0%
CGCCTAATGAAAGTCGACAG+AGG + Chr1:86014767-86014786 MsG0180005035.01.T01:CDS 50.0%
TGAGATTTGGAGGTGGTTGC+AGG - Chr1:86013711-86013730 None:intergenic 50.0%
TGGTGGGAAGAGAAGATCCA+AGG - Chr1:86013611-86013630 None:intergenic 50.0%
TGTTGACGTTGATACCGAGG+CGG + Chr1:86013946-86013965 MsG0180005035.01.T01:CDS 50.0%
AGTGGCGTGAGAGAGACGAA+AGG - Chr1:86013645-86013664 None:intergenic 55.0%
CACCTCCAAATCTCAACCGG+CGG + Chr1:86013716-86013735 MsG0180005035.01.T01:CDS 55.0%
GAAAGTGAGAGGAGCGACAG+TGG - Chr1:86013663-86013682 None:intergenic 55.0%
GAACGATGAAGCCACCTAGC+AGG - Chr1:86013872-86013891 None:intergenic 55.0%
GACCACGAGCTCAAAGGTGA+AGG + Chr1:86013818-86013837 MsG0180005035.01.T01:CDS 55.0%
GATTTGGAGGTGGTTGCAGG+CGG - Chr1:86013708-86013727 None:intergenic 55.0%
GCCACTCACTCATCCATGCT+TGG + Chr1:86013560-86013579 MsG0180005035.01.T01:exon 55.0%
TCCGGGAAAACAGCTGGAGT+CGG - Chr1:86013977-86013996 None:intergenic 55.0%
TGGCACGCGATTACTCCTTG+CGG + Chr1:86013755-86013774 MsG0180005035.01.T01:CDS 55.0%
! GAAGCATCACACCGCTGCTT+CGG - Chr1:86013793-86013812 None:intergenic 55.0%
!! GGTATAGGGGTGCTTGCTGA+TGG + Chr1:86014688-86014707 MsG0180005035.01.T01:intron 55.0%
!! GTACAGGGATTGGTTGGTGG+TGG + Chr1:86014277-86014296 MsG0180005035.01.T01:intron 55.0%
!! TAGGGGTGCTTGCTGATGGT+CGG + Chr1:86014692-86014711 MsG0180005035.01.T01:intron 55.0%
!! TGGTTGGTGGTGGTGGTGTT+TGG + Chr1:86014287-86014306 MsG0180005035.01.T01:intron 55.0%
ATTACTCCTTGCGGCGGCGA+TGG + Chr1:86013764-86013783 MsG0180005035.01.T01:CDS 60.0%
CAACAATAACCGGCGGCGCA+AGG - Chr1:86013931-86013950 None:intergenic 60.0%
CACGCGATTACTCCTTGCGG+CGG + Chr1:86013758-86013777 MsG0180005035.01.T01:CDS 60.0%
CGTGAGAGAGACGAAAGGCG+TGG - Chr1:86013640-86013659 None:intergenic 60.0%
CTCCAAATCTCAACCGGCGG+TGG + Chr1:86013719-86013738 MsG0180005035.01.T01:CDS 60.0%
CTCTCGTCCGGGAAAACAGC+TGG - Chr1:86013983-86014002 None:intergenic 60.0%
CTTGGGATGCTCGTCCTGCT+AGG + Chr1:86013855-86013874 MsG0180005035.01.T01:CDS 60.0%
GATCCAAGGACGAGAGCGAG+TGG - Chr1:86013597-86013616 None:intergenic 60.0%
GCGGTGGAGCTGCTTCGATA+TGG + Chr1:86013735-86013754 MsG0180005035.01.T01:CDS 60.0%
GGCGCAGAGTACACCAAGCA+TGG - Chr1:86013576-86013595 None:intergenic 60.0%
GGGAAAACAGCTGGAGTCGG+CGG - Chr1:86013974-86013993 None:intergenic 60.0%
TATCGAAGCAGCTCCACCGC+CGG - Chr1:86013735-86013754 None:intergenic 60.0%
TTCCCGGACGAGAGTTCCGA+GGG + Chr1:86013989-86014008 MsG0180005035.01.T01:CDS 60.0%
TTTCCCGGACGAGAGTTCCG+AGG + Chr1:86013988-86014007 MsG0180005035.01.T01:CDS 60.0%
! AGAGACGAAAGGCGTGGTGG+TGG - Chr1:86013634-86013653 None:intergenic 60.0%
! CACCGCCGGTTGAGATTTGG+AGG - Chr1:86013721-86013740 None:intergenic 60.0%
! CGCCGGTTGAGATTTGGAGG+TGG - Chr1:86013718-86013737 None:intergenic 60.0%
! CTCCACCGCCGGTTGAGATT+TGG - Chr1:86013724-86013743 None:intergenic 60.0%
! GAGAGAGACGAAAGGCGTGG+TGG - Chr1:86013637-86013656 None:intergenic 60.0%
! GGCGATGGATTCCGAAGCAG+CGG + Chr1:86013779-86013798 MsG0180005035.01.T01:CDS 60.0%
!! AAAGGCGTGGTGGTGGTGGT+GGG - Chr1:86013627-86013646 None:intergenic 60.0%
!! CAGGGATTGGTTGGTGGTGG+TGG + Chr1:86014280-86014299 MsG0180005035.01.T01:intron 60.0%
!! GCCGACTCCAGCTGTTTTCC+CGG + Chr1:86013973-86013992 MsG0180005035.01.T01:CDS 60.0%
CGTCCCTCGGAACTCTCGTC+CGG - Chr1:86013995-86014014 None:intergenic 65.0%
GGGATGCTCGTCCTGCTAGG+TGG + Chr1:86013858-86013877 MsG0180005035.01.T01:CDS 65.0%
GGTGGTTGCAGGCGGTGGAA+TGG - Chr1:86013700-86013719 None:intergenic 65.0%
GGTTGCAGGCGGTGGAATGG+TGG - Chr1:86013697-86013716 None:intergenic 65.0%
GTCCCTCGGAACTCTCGTCC+GGG - Chr1:86013994-86014013 None:intergenic 65.0%
TGCAGGCGGTGGAATGGTGG+CGG - Chr1:86013694-86013713 None:intergenic 65.0%
TTGGAGGTGGTTGCAGGCGG+TGG - Chr1:86013705-86013724 None:intergenic 65.0%
!! GAAAGGCGTGGTGGTGGTGG+TGG - Chr1:86013628-86013647 None:intergenic 65.0%
!! GACGAAAGGCGTGGTGGTGG+TGG - Chr1:86013631-86013650 None:intergenic 65.0%
CGGAATCCATCGCCGCCGCA+AGG - Chr1:86013773-86013792 None:intergenic 70.0%
GCGCCACTCGCTCTCGTCCT+TGG + Chr1:86013591-86013610 MsG0180005035.01.T01:CDS 70.0%
!! AGGCGGTGGAATGGTGGCGG+CGG - Chr1:86013691-86013710 None:intergenic 70.0%
CGGCGGGCGGGGAAAGTGAG+AGG - Chr1:86013674-86013693 None:intergenic 75.0%
CTGCGCTTGCCTTGCGCCGC+CGG + Chr1:86013919-86013938 MsG0180005035.01.T01:CDS 75.0%
!! GGCGGTGGAATGGTGGCGGC+GGG - Chr1:86013690-86013709 None:intergenic 75.0%
!! GGTGGAATGGTGGCGGCGGG+CGG - Chr1:86013687-86013706 None:intergenic 75.0%
!! GTGGAATGGTGGCGGCGGGC+GGG - Chr1:86013686-86013705 None:intergenic 75.0%
!! TGGAATGGTGGCGGCGGGCG+GGG - Chr1:86013685-86013704 None:intergenic 75.0%
! TGGAGTCGGCGGCGCCGCCT+CGG - Chr1:86013963-86013982 None:intergenic 80.0%
Chromosome Type Strat End Strand Name
Chr1 gene 86013354 86015342 86013354 ID=MsG0180005035.01;Name=MsG0180005035.01
Chr1 mRNA 86013354 86015342 86013354 ID=MsG0180005035.01.T01;Parent=MsG0180005035.01;Name=MsG0180005035.01.T01;_AED=0.31;_eAED=0.31;_QI=221|1|1|1|1|1|4|168|256
Chr1 exon 86013354 86014079 86013354 ID=MsG0180005035.01.T01:exon:9828;Parent=MsG0180005035.01.T01
Chr1 exon 86014695 86014816 86014695 ID=MsG0180005035.01.T01:exon:9829;Parent=MsG0180005035.01.T01
Chr1 exon 86014931 86014971 86014931 ID=MsG0180005035.01.T01:exon:9830;Parent=MsG0180005035.01.T01
Chr1 exon 86015072 86015342 86015072 ID=MsG0180005035.01.T01:exon:9831;Parent=MsG0180005035.01.T01
Chr1 five_prime_UTR 86013354 86013574 86013354 ID=MsG0180005035.01.T01:five_prime_utr;Parent=MsG0180005035.01.T01
Chr1 CDS 86013575 86014079 86013575 ID=MsG0180005035.01.T01:cds;Parent=MsG0180005035.01.T01
Chr1 CDS 86014695 86014816 86014695 ID=MsG0180005035.01.T01:cds;Parent=MsG0180005035.01.T01
Chr1 CDS 86014931 86014971 86014931 ID=MsG0180005035.01.T01:cds;Parent=MsG0180005035.01.T01
Chr1 CDS 86015072 86015174 86015072 ID=MsG0180005035.01.T01:cds;Parent=MsG0180005035.01.T01
Chr1 three_prime_UTR 86015175 86015342 86015175 ID=MsG0180005035.01.T01:three_prime_utr;Parent=MsG0180005035.01.T01
Gene Sequence

>MsG0180005035.01.T01

ATGCTTGGTGTACTCTGCGCCACTCGCTCTCGTCCTTGGATCTTCTCTTCCCACCACCACCACCACGCCTTTCGTCTCTCTCACGCCACTGTCGCTCCTCTCACTTTCCCCGCCCGCCGCCACCATTCCACCGCCTGCAACCACCTCCAAATCTCAACCGGCGGTGGAGCTGCTTCGATATGGCACGCGATTACTCCTTGCGGCGGCGATGGATTCCGAAGCAGCGGTGTGATGCTTCATCATGACCACGAGCTCAAAGGTGAAGGATCGTGGAACGTTGCTTGGGATGCTCGTCCTGCTAGGTGGCTTCATCGTTCTGACTCAGCTTGGCTTCTTTTCGGTGTCTGCGCTTGCCTTGCGCCGCCGGTTATTGTTGACGTTGATACCGAGGCGGCGCCGCCGACTCCAGCTGTTTTCCCGGACGAGAGTTCCGAGGGACGTGAAGTGAAAGATGAGCTTTCCGATGAACGTGACGATGAACTCAACGCTGATTACAGAGTCACGGGGGTGCTTGCTGATGGTCGGTGTCTGTTCAGAGCAATAGCTCATGGAGCTTGCTTGAAAAATGGAGAGGAAGCGCCTAATGAAAGTCGACAGAGGGAACTTGCTGACGAATTAAGGGTTAAAGTTGCTGAAGAACTTTTGAACAGGCGCAAGGAAACAGAATGGTTTATAGAAGGGGATTTTGACACATATGTGACCAGAATTCAACAGACTTATGTTTGGGGAGGAGAGCCTGAGCTGTTAATGGCTTCTCATGTTTTGAAGTGA
Protein sequence

>MsG0180005035.01.T01

MLGVLCATRSRPWIFSSHHHHHAFRLSHATVAPLTFPARRHHSTACNHLQISTGGGAASIWHAITPCGGDGFRSSGVMLHHDHELKGEGSWNVAWDARPARWLHRSDSAWLLFGVCACLAPPVIVDVDTEAAPPTPAVFPDESSEGREVKDELSDERDDELNADYRVTGVLADGRCLFRAIAHGACLKNGEEAPNESRQRELADELRVKVAEELLNRRKETEWFIEGDFDTYVTRIQQTYVWGGEPELLMASHVLK*