AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0280007423.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280007423.01.T01 MTR_2g025050 90.152 132 13 0 1 132 1 132 1.39e-81 243
MsG0280007423.01.T01 MTR_2g025040 76.296 135 25 2 1 132 1 131 2.76e-63 196
MsG0280007423.01.T01 MTR_1g099880 74.026 77 16 1 56 132 3 75 3.60e-30 108
MsG0280007423.01.T01 MTR_5g029590 43.902 82 35 2 62 132 91 172 1.96e-11 60.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280007423.01.T01 AT4G04020 54.217 83 28 3 57 132 54 133 1.93e-18 79.7
MsG0280007423.01.T01 AT4G22240 51.042 96 35 4 41 132 38 125 2.38e-17 76.6
MsG0280007423.01.T01 AT2G35490 50.704 71 33 2 62 132 124 192 5.90e-13 64.7

Find 41 sgRNAs with CRISPR-Local

Find 43 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGAGGCGGGCGGTGTATGTT+CGG 0.309347 2:-15219305 MsG0280007423.01.T01:CDS
GTGGAGTTGAGAGAGGTTAC+TGG 0.345185 2:+15219408 None:intergenic
TTCTGCTGGCGTCGCGGTTG+CGG 0.388728 2:-15219247 MsG0280007423.01.T01:CDS
GAGGCGGGCGGTGTATGTTC+GGG 0.431419 2:-15219304 MsG0280007423.01.T01:CDS
TGGGGTCCAGAGCCTTCTGC+TGG 0.436696 2:-15219261 MsG0280007423.01.T01:CDS
TTGGTTGAGGTTTGAGAGAG+AGG 0.455247 2:+15219452 None:intergenic
GGGTGTTTGTGTGGAGAAGT+TGG 0.455512 2:+15219433 None:intergenic
TTGTGTGGAGAAGTTGGTTG+AGG 0.470556 2:+15219439 None:intergenic
TGAAGGTGTGGAGTTGAGAG+AGG 0.491712 2:+15219401 None:intergenic
AGAAAGTGACGGATAGTGAG+AGG 0.496240 2:-15219209 MsG0280007423.01.T01:CDS
GCGACGCCAGCAGAAGGCTC+TGG 0.497720 2:+15219255 None:intergenic
AAACCATCGGCCAACACAAT+CGG 0.499127 2:+15219327 None:intergenic
AAACCGAACGTGATGGTCGA+TGG 0.504270 2:+15219372 None:intergenic
GTTGAGAGAGGTTACTGGAA+GGG 0.505605 2:+15219413 None:intergenic
TGCTGGCGTCGCGGTTGCGG+AGG 0.508923 2:-15219244 MsG0280007423.01.T01:CDS
TCTCCATCGACCATCACGTT+CGG 0.514451 2:-15219375 MsG0280007423.01.T01:CDS
TTGTGTTGGCCGATGGTTTG+AGG 0.521688 2:-15219323 MsG0280007423.01.T01:CDS
AGACCGATTGTGTTGGCCGA+TGG 0.523427 2:-15219330 MsG0280007423.01.T01:CDS
GTTGGCCGATGGTTTGAGGC+GGG 0.530141 2:-15219319 MsG0280007423.01.T01:CDS
TAATACAAATGATGATGAGT+GGG 0.532459 2:-15219280 MsG0280007423.01.T01:CDS
CACCGCCCGCCTCAAACCAT+CGG 0.549599 2:+15219314 None:intergenic
GGCATCAGTAGAAGCGGTGT+GGG 0.550175 2:+15219069 None:intergenic
TGAGAGGGAGAAGCTGAAGA+AGG 0.553126 2:-15219193 MsG0280007423.01.T01:CDS
GGAGAAGCTGAAGAAGGCGT+TGG 0.553210 2:-15219187 MsG0280007423.01.T01:CDS
GCAACCGCGACGCCAGCAGA+AGG 0.556346 2:+15219249 None:intergenic
AAGCTGAAGAAGGCGTTGGT+TGG 0.574482 2:-15219183 MsG0280007423.01.T01:CDS
TTACTGGAAGGGTGTTTGTG+TGG 0.583482 2:+15219424 None:intergenic
AGTTGAGAGAGGTTACTGGA+AGG 0.592004 2:+15219412 None:intergenic
CGATGGAGAAGTTGAAGGTG+TGG 0.601344 2:+15219389 None:intergenic
GCTCAGAAAACCGAACGTGA+TGG 0.601660 2:+15219365 None:intergenic
GGCCGATGGTTTGAGGCGGG+CGG 0.626348 2:-15219316 MsG0280007423.01.T01:CDS
CTAATACAAATGATGATGAG+TGG 0.629749 2:-15219281 MsG0280007423.01.T01:CDS
ATGGTCGATGGAGAAGTTGA+AGG 0.631836 2:+15219384 None:intergenic
GAAAGTGACGGATAGTGAGA+GGG 0.635117 2:-15219208 MsG0280007423.01.T01:CDS
TGTCAGAAGACCGATTGTGT+TGG 0.635862 2:-15219337 MsG0280007423.01.T01:CDS
ACTCAAGGCATCAGTAGAAG+CGG 0.637283 2:+15219063 None:intergenic
AGGCATCAGTAGAAGCGGTG+TGG 0.641827 2:+15219068 None:intergenic
AGAGCCTTCTGCTGGCGTCG+CGG 0.645840 2:-15219253 MsG0280007423.01.T01:CDS
GAAAGAGACTGAGAAAGTGA+CGG 0.653391 2:-15219220 MsG0280007423.01.T01:CDS
TGTTGGCCGATGGTTTGAGG+CGG 0.676008 2:-15219320 MsG0280007423.01.T01:CDS
AATACAAATGATGATGAGTG+GGG 0.724716 2:-15219279 MsG0280007423.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! TAATACAAATGATGATGAGT+GGG - Chr2:15219241-15219260 MsG0280007423.01.T01:CDS 25.0%
! AATACAAATGATGATGAGTG+GGG - Chr2:15219242-15219261 MsG0280007423.01.T01:CDS 30.0%
! CTAATACAAATGATGATGAG+TGG - Chr2:15219240-15219259 MsG0280007423.01.T01:CDS 30.0%
GAAAGAGACTGAGAAAGTGA+CGG - Chr2:15219301-15219320 MsG0280007423.01.T01:CDS 40.0%
!!! CGTTGGTTGGTTCGTTTTAT+GGG - Chr2:15219351-15219370 MsG0280007423.01.T01:CDS 40.0%
AAACCATCGGCCAACACAAT+CGG + Chr2:15219197-15219216 None:intergenic 45.0%
AGTTGAGAGAGGTTACTGGA+AGG + Chr2:15219112-15219131 None:intergenic 45.0%
ATGGTCGATGGAGAAGTTGA+AGG + Chr2:15219140-15219159 None:intergenic 45.0%
GTTGAGAGAGGTTACTGGAA+GGG + Chr2:15219111-15219130 None:intergenic 45.0%
TGTCAGAAGACCGATTGTGT+TGG - Chr2:15219184-15219203 MsG0280007423.01.T01:CDS 45.0%
TTGGTTGAGGTTTGAGAGAG+AGG + Chr2:15219072-15219091 None:intergenic 45.0%
! TCGTTTTATGGGACTGATCG+TGG - Chr2:15219362-15219381 MsG0280007423.01.T01:CDS 45.0%
! TTGTGTGGAGAAGTTGGTTG+AGG + Chr2:15219085-15219104 None:intergenic 45.0%
!! AGAAAGTGACGGATAGTGAG+AGG - Chr2:15219312-15219331 MsG0280007423.01.T01:CDS 45.0%
!! GAAAGTGACGGATAGTGAGA+GGG - Chr2:15219313-15219332 MsG0280007423.01.T01:CDS 45.0%
!! TTACTGGAAGGGTGTTTGTG+TGG + Chr2:15219100-15219119 None:intergenic 45.0%
!!! GCGTTGGTTGGTTCGTTTTA+TGG - Chr2:15219350-15219369 MsG0280007423.01.T01:CDS 45.0%
AAACCGAACGTGATGGTCGA+TGG + Chr2:15219152-15219171 None:intergenic 50.0%
CGATGGAGAAGTTGAAGGTG+TGG + Chr2:15219135-15219154 None:intergenic 50.0%
GCTCAGAAAACCGAACGTGA+TGG + Chr2:15219159-15219178 None:intergenic 50.0%
GGGTGTTTGTGTGGAGAAGT+TGG + Chr2:15219091-15219110 None:intergenic 50.0%
GTGGAGTTGAGAGAGGTTAC+TGG + Chr2:15219116-15219135 None:intergenic 50.0%
TCTCCATCGACCATCACGTT+CGG - Chr2:15219146-15219165 MsG0280007423.01.T01:CDS 50.0%
TGAGAGGGAGAAGCTGAAGA+AGG - Chr2:15219328-15219347 MsG0280007423.01.T01:CDS 50.0%
! TGAAGGTGTGGAGTTGAGAG+AGG + Chr2:15219123-15219142 None:intergenic 50.0%
! TTGTGTTGGCCGATGGTTTG+AGG - Chr2:15219198-15219217 MsG0280007423.01.T01:CDS 50.0%
!! AAGCTGAAGAAGGCGTTGGT+TGG - Chr2:15219338-15219357 MsG0280007423.01.T01:CDS 50.0%
AGGCATCAGTAGAAGCGGTG+TGG + Chr2:15219456-15219475 None:intergenic 55.0%
GGCATCAGTAGAAGCGGTGT+GGG + Chr2:15219455-15219474 None:intergenic 55.0%
TGTTGGCCGATGGTTTGAGG+CGG - Chr2:15219201-15219220 MsG0280007423.01.T01:CDS 55.0%
! AGACCGATTGTGTTGGCCGA+TGG - Chr2:15219191-15219210 MsG0280007423.01.T01:CDS 55.0%
! GGAGAAGCTGAAGAAGGCGT+TGG - Chr2:15219334-15219353 MsG0280007423.01.T01:CDS 55.0%
TGAGGCGGGCGGTGTATGTT+CGG - Chr2:15219216-15219235 MsG0280007423.01.T01:CDS 60.0%
! GTTGGCCGATGGTTTGAGGC+GGG - Chr2:15219202-15219221 MsG0280007423.01.T01:CDS 60.0%
AGAGCCTTCTGCTGGCGTCG+CGG - Chr2:15219268-15219287 MsG0280007423.01.T01:CDS 65.0%
CACCGCCCGCCTCAAACCAT+CGG + Chr2:15219210-15219229 None:intergenic 65.0%
GAGGCGGGCGGTGTATGTTC+GGG - Chr2:15219217-15219236 MsG0280007423.01.T01:CDS 65.0%
TGGGGTCCAGAGCCTTCTGC+TGG - Chr2:15219260-15219279 MsG0280007423.01.T01:CDS 65.0%
TTCTGCTGGCGTCGCGGTTG+CGG - Chr2:15219274-15219293 MsG0280007423.01.T01:CDS 65.0%
GCAACCGCGACGCCAGCAGA+AGG + Chr2:15219275-15219294 None:intergenic 70.0%
GCGACGCCAGCAGAAGGCTC+TGG + Chr2:15219269-15219288 None:intergenic 70.0%
! GGCCGATGGTTTGAGGCGGG+CGG - Chr2:15219205-15219224 MsG0280007423.01.T01:CDS 70.0%
TGCTGGCGTCGCGGTTGCGG+AGG - Chr2:15219277-15219296 MsG0280007423.01.T01:CDS 75.0%
Chromosome Type Strat End Strand Name
Chr2 gene 15219065 15219478 15219065 ID=MsG0280007423.01;Name=MsG0280007423.01
Chr2 mRNA 15219065 15219478 15219065 ID=MsG0280007423.01.T01;Parent=MsG0280007423.01;Name=MsG0280007423.01.T01;_AED=0.42;_eAED=0.42;_QI=0|-1|0|1|-1|1|1|0|137
Chr2 exon 15219065 15219478 15219065 ID=MsG0280007423.01.T01:exon:1020;Parent=MsG0280007423.01.T01
Chr2 CDS 15219065 15219478 15219065 ID=MsG0280007423.01.T01:cds;Parent=MsG0280007423.01.T01
Gene Sequence

>MsG0280007423.01.T01

ATGGCCTCTCTCTCAAACCTCAACCAACTTCTCCACACAAACACCCTTCCAGTAACCTCTCTCAACTCCACACCTTCAACTTCTCCATCGACCATCACGTTCGGTTTTCTGAGCAAACATGTCAGAAGACCGATTGTGTTGGCCGATGGTTTGAGGCGGGCGGTGTATGTTCGGGCTAATACAAATGATGATGAGTGGGGTCCAGAGCCTTCTGCTGGCGTCGCGGTTGCGGAGGTGAAAGAGACTGAGAAAGTGACGGATAGTGAGAGGGAGAAGCTGAAGAAGGCGTTGGTTGGTTCGTTTTATGGGACTGATCGTGGATTGAAAGCGACGAGTGAAACGAGAGCTGAGATTGTTGAGCTGATTACTCAGCTTGAAGCTAAGAATCCCACACCGCTTCTACTGATGCCTTGA

Protein sequence

>MsG0280007423.01.T01

MASLSNLNQLLHTNTLPVTSLNSTPSTSPSTITFGFLSKHVRRPIVLADGLRRAVYVRANTNDDEWGPEPSAGVAVAEVKETEKVTDSEREKLKKALVGSFYGTDRGLKATSETRAEIVELITQLEAKNPTPLLLMP*