Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180005075.01.T01 | XP_024628654.1 | 69.492 | 118 | 36 | 0 | 1 | 118 | 1 | 118 | 1.09E-48 | 166 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180005075.01.T01 | A0A392LXD1 | 66.102 | 118 | 40 | 0 | 1 | 118 | 406 | 523 | 9.65e-44 | 162 |
| MsG0180005075.01.T01 | A0A392MVR0 | 62.712 | 118 | 44 | 0 | 1 | 118 | 1 | 118 | 5.03e-46 | 155 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180005075.01 | MsG0180005078.01 | 0.831409 | 1.697305e-55 | 1.840181e-52 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180005075.01.T01 | MTR_1g061220 | 61.017 | 118 | 46 | 0 | 1 | 118 | 2462 | 2579 | 1.39e-45 | 157 |
| MsG0180005075.01.T01 | MTR_6g024380 | 59.322 | 118 | 48 | 0 | 1 | 118 | 1 | 118 | 7.24e-44 | 149 |
| MsG0180005075.01.T01 | MTR_6g016175 | 54.237 | 118 | 50 | 1 | 1 | 118 | 1 | 114 | 1.12e-34 | 125 |
| MsG0180005075.01.T01 | MTR_3g022950 | 52.679 | 112 | 53 | 0 | 3 | 114 | 636 | 747 | 4.67e-34 | 124 |
| MsG0180005075.01.T01 | MTR_1g068728 | 61.702 | 94 | 36 | 0 | 1 | 94 | 1 | 94 | 1.53e-32 | 114 |
| MsG0180005075.01.T01 | MTR_7g083690 | 45.763 | 118 | 64 | 0 | 1 | 118 | 604 | 721 | 6.76e-32 | 118 |
| MsG0180005075.01.T01 | MTR_6g033705 | 60.000 | 85 | 34 | 0 | 34 | 118 | 25 | 109 | 2.62e-31 | 111 |
| MsG0180005075.01.T01 | MTR_0032s0090 | 45.690 | 116 | 63 | 0 | 3 | 118 | 1 | 116 | 3.84e-31 | 110 |
| MsG0180005075.01.T01 | MTR_7g057470 | 46.610 | 118 | 63 | 0 | 1 | 118 | 210 | 327 | 1.82e-30 | 114 |
| MsG0180005075.01.T01 | MTR_7g057490 | 46.610 | 118 | 63 | 0 | 1 | 118 | 210 | 327 | 1.82e-30 | 114 |
| MsG0180005075.01.T01 | MTR_2g105880 | 48.624 | 109 | 56 | 0 | 10 | 118 | 67 | 175 | 2.74e-30 | 110 |
| MsG0180005075.01.T01 | MTR_5g095550 | 51.456 | 103 | 50 | 0 | 16 | 118 | 234 | 336 | 1.05e-28 | 107 |
| MsG0180005075.01.T01 | MTR_1g033770 | 44.068 | 118 | 50 | 1 | 1 | 118 | 214 | 315 | 6.23e-24 | 95.1 |
| MsG0180005075.01.T01 | MTR_5g054740 | 51.852 | 81 | 39 | 0 | 1 | 81 | 790 | 870 | 1.91e-22 | 91.3 |
| MsG0180005075.01.T01 | MTR_7g103360 | 51.613 | 93 | 45 | 0 | 7 | 99 | 73 | 165 | 6.02e-22 | 89.4 |
| MsG0180005075.01.T01 | MTR_0426s0010 | 49.438 | 89 | 45 | 0 | 1 | 89 | 22 | 110 | 6.07e-22 | 87.4 |
| MsG0180005075.01.T01 | MTR_3g448380 | 38.983 | 118 | 71 | 1 | 1 | 118 | 68 | 184 | 3.42e-21 | 85.9 |
| MsG0180005075.01.T01 | MTR_6g048840 | 55.224 | 67 | 30 | 0 | 52 | 118 | 208 | 274 | 7.45e-19 | 80.9 |
| MsG0180005075.01.T01 | MTR_0062s0080 | 37.736 | 106 | 61 | 1 | 12 | 117 | 204 | 304 | 9.18e-19 | 80.9 |
| MsG0180005075.01.T01 | MTR_0062s0050 | 37.736 | 106 | 61 | 1 | 12 | 117 | 204 | 304 | 9.98e-19 | 80.9 |
| MsG0180005075.01.T01 | MTR_0938s0010 | 37.895 | 95 | 59 | 0 | 23 | 117 | 4 | 98 | 1.36e-18 | 79.0 |
| MsG0180005075.01.T01 | MTR_8g058470 | 45.122 | 82 | 45 | 0 | 37 | 118 | 341 | 422 | 2.45e-18 | 79.7 |
| MsG0180005075.01.T01 | MTR_7g108760 | 45.122 | 82 | 45 | 0 | 37 | 118 | 343 | 424 | 2.64e-18 | 79.3 |
| MsG0180005075.01.T01 | MTR_4g054200 | 56.452 | 62 | 27 | 0 | 12 | 73 | 133 | 194 | 1.09e-15 | 71.2 |
| MsG0180005075.01.T01 | MTR_5g021380 | 58.491 | 53 | 20 | 1 | 66 | 116 | 569 | 621 | 6.56e-14 | 67.0 |
| MsG0180005075.01.T01 | MTR_0128s0030 | 39.506 | 81 | 48 | 1 | 2 | 81 | 45 | 125 | 8.99e-14 | 63.2 |
| MsG0180005075.01.T01 | MTR_7g053420 | 46.575 | 73 | 34 | 1 | 6 | 78 | 111 | 178 | 1.39e-13 | 65.5 |
| MsG0180005075.01.T01 | MTR_7g023030 | 44.286 | 70 | 29 | 1 | 45 | 114 | 84 | 143 | 2.58e-11 | 58.5 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 27 sgRNAs with CRISPR-Local
Find 40 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGGTGGTATCTTGGAGTGTT+AGG | 0.288408 | 1:-86560299 | MsG0180005075.01.T01:CDS |
| CATGTTGTTGGTTGGGGAGA+AGG | 0.347215 | 1:-86560244 | MsG0180005075.01.T01:CDS |
| AGGAGGTCATGTTGTTGGTT+GGG | 0.360833 | 1:-86560251 | MsG0180005075.01.T01:CDS |
| GGTGGTATCTTGGAGTGTTA+GGG | 0.360910 | 1:-86560298 | MsG0180005075.01.T01:CDS |
| CAGCGGTTTGTTGAAGCGTC+AGG | 0.400077 | 1:-86560123 | MsG0180005075.01.T01:CDS |
| GTAGGATGAAGGTGGTATCT+TGG | 0.406890 | 1:-86560308 | None:intergenic |
| CAGGAGGTCTGTCGAGGGTG+TGG | 0.409959 | 1:-86560104 | MsG0180005075.01.T01:CDS |
| GAGAAAGGAGGTCATGTTGT+TGG | 0.436184 | 1:-86560256 | MsG0180005075.01.T01:CDS |
| GAACAAGTAAAACTCCTCAT+TGG | 0.440471 | 1:+86560005 | None:intergenic |
| GGAGGTCATGTTGTTGGTTG+GGG | 0.440977 | 1:-86560250 | MsG0180005075.01.T01:CDS |
| AAGGAGGTCATGTTGTTGGT+TGG | 0.456093 | 1:-86560252 | MsG0180005075.01.T01:CDS |
| TGGTATCTTGGAGTGTTAGG+GGG | 0.490687 | 1:-86560296 | MsG0180005075.01.T01:CDS |
| AATTGAATATACAAGATAAA+CGG | 0.491588 | 1:+86560219 | None:intergenic |
| GTGGTATCTTGGAGTGTTAG+GGG | 0.498628 | 1:-86560297 | MsG0180005075.01.T01:CDS |
| AGGAGGTCTGTCGAGGGTGT+GGG | 0.538531 | 1:-86560103 | MsG0180005075.01.T01:CDS |
| AAGCGTCAGGAGGTCTGTCG+AGG | 0.548829 | 1:-86560110 | MsG0180005075.01.T01:CDS |
| TTGTGATCATCAAGCGAAGT+AGG | 0.555067 | 1:-86559968 | None:intergenic |
| GGGTGGGTTAGAGAAGAGAA+AGG | 0.565139 | 1:-86560271 | MsG0180005075.01.T01:CDS |
| TGGGTTAGAGAAGAGAAAGG+AGG | 0.599929 | 1:-86560268 | MsG0180005075.01.T01:CDS |
| CGGTTTGTTGAAGCGTCAGG+AGG | 0.617794 | 1:-86560120 | MsG0180005075.01.T01:CDS |
| AGCGTCAGGAGGTCTGTCGA+GGG | 0.620749 | 1:-86560109 | MsG0180005075.01.T01:CDS |
| TAGGTTCTTTAAATCCAATG+AGG | 0.626140 | 1:-86560019 | MsG0180005075.01.T01:CDS |
| GAGGTTTGGACCTCTGTCAG+TGG | 0.634966 | 1:-86560066 | MsG0180005075.01.T01:CDS |
| TACTTCGCTTGATGATCACA+AGG | 0.653682 | 1:+86559970 | None:intergenic |
| GGAGGTATGTAGGATGAAGG+TGG | 0.658369 | 1:-86560316 | None:intergenic |
| AAAGTGATCACCACTGACAG+AGG | 0.664604 | 1:+86560056 | None:intergenic |
| AGGGACATTACCCCACAATG+AGG | 0.685236 | 1:+86560161 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATTGAATATACAAGATAAA+CGG | + | Chr1:86560056-86560075 | None:intergenic | 15.0% |
| !! | ATAAAAAATCATCACATAAC+TGG | + | Chr1:86560088-86560107 | None:intergenic | 20.0% |
| TAGGTTCTTTAAATCCAATG+AGG | - | Chr1:86560253-86560272 | MsG0180005075.01.T01:CDS | 30.0% | |
| !!! | ATATTTTTGAAGTGGAGGTT+TGG | - | Chr1:86560192-86560211 | MsG0180005075.01.T01:CDS | 30.0% |
| !!! | ATTTTTTATGTGCCTCATTG+TGG | - | Chr1:86560099-86560118 | MsG0180005075.01.T01:CDS | 30.0% |
| !!! | GATCACTTTTTGATGATACA+CGG | - | Chr1:86560230-86560249 | MsG0180005075.01.T01:CDS | 30.0% |
| !!! | TTTTTTATGTGCCTCATTGT+GGG | - | Chr1:86560100-86560119 | MsG0180005075.01.T01:CDS | 30.0% |
| GAACAAGTAAAACTCCTCAT+TGG | + | Chr1:86560270-86560289 | None:intergenic | 35.0% | |
| ! | GCTGATAAGAAAAAGCACAA+GGG | + | Chr1:86560133-86560152 | None:intergenic | 35.0% |
| ! | TTTTTATGTGCCTCATTGTG+GGG | - | Chr1:86560101-86560120 | MsG0180005075.01.T01:CDS | 35.0% |
| !! | ACTTTTTGATGATACACGGT+AGG | - | Chr1:86560234-86560253 | MsG0180005075.01.T01:CDS | 35.0% |
| !!! | CTTGTGCTTTTTCTTATCAG+CGG | - | Chr1:86560132-86560151 | MsG0180005075.01.T01:CDS | 35.0% |
| ! | CGCTGATAAGAAAAAGCACA+AGG | + | Chr1:86560134-86560153 | None:intergenic | 40.0% |
| ! | TACTTCGCTTGATGATCACA+AGG | + | Chr1:86560305-86560324 | None:intergenic | 40.0% |
| !! | GGTGTGGGATATTTTTGAAG+TGG | - | Chr1:86560184-86560203 | MsG0180005075.01.T01:CDS | 40.0% |
| !!! | GTGGGATATTTTTGAAGTGG+AGG | - | Chr1:86560187-86560206 | MsG0180005075.01.T01:CDS | 40.0% |
| AAAGTGATCACCACTGACAG+AGG | + | Chr1:86560219-86560238 | None:intergenic | 45.0% | |
| AAGGAGGTCATGTTGTTGGT+TGG | - | Chr1:86560020-86560039 | MsG0180005075.01.T01:CDS | 45.0% | |
| GAGAAAGGAGGTCATGTTGT+TGG | - | Chr1:86560016-86560035 | MsG0180005075.01.T01:CDS | 45.0% | |
| ! | AGGAGGTCATGTTGTTGGTT+GGG | - | Chr1:86560021-86560040 | MsG0180005075.01.T01:CDS | 45.0% |
| ! | AGGTGGTATCTTGGAGTGTT+AGG | - | Chr1:86559973-86559992 | MsG0180005075.01.T01:CDS | 45.0% |
| ! | GGTGGTATCTTGGAGTGTTA+GGG | - | Chr1:86559974-86559993 | MsG0180005075.01.T01:CDS | 45.0% |
| ! | GTGGTATCTTGGAGTGTTAG+GGG | - | Chr1:86559975-86559994 | MsG0180005075.01.T01:CDS | 45.0% |
| ! | TGGGTTAGAGAAGAGAAAGG+AGG | - | Chr1:86560004-86560023 | MsG0180005075.01.T01:CDS | 45.0% |
| ! | TGGTATCTTGGAGTGTTAGG+GGG | - | Chr1:86559976-86559995 | MsG0180005075.01.T01:CDS | 45.0% |
| AGGGACATTACCCCACAATG+AGG | + | Chr1:86560114-86560133 | None:intergenic | 50.0% | |
| ! | ATCTTGGAGTGTTAGGGGGT+TGG | - | Chr1:86559980-86559999 | MsG0180005075.01.T01:CDS | 50.0% |
| ! | CATGTTGTTGGTTGGGGAGA+AGG | - | Chr1:86560028-86560047 | MsG0180005075.01.T01:CDS | 50.0% |
| ! | GGAGGTCATGTTGTTGGTTG+GGG | - | Chr1:86560022-86560041 | MsG0180005075.01.T01:CDS | 50.0% |
| ! | TCTTGGAGTGTTAGGGGGTT+GGG | - | Chr1:86559981-86560000 | MsG0180005075.01.T01:CDS | 50.0% |
| !! | GGGTGGGTTAGAGAAGAGAA+AGG | - | Chr1:86560001-86560020 | MsG0180005075.01.T01:CDS | 50.0% |
| GAGGTTTGGACCTCTGTCAG+TGG | - | Chr1:86560206-86560225 | MsG0180005075.01.T01:CDS | 55.0% | |
| !! | CAGCGGTTTGTTGAAGCGTC+AGG | - | Chr1:86560149-86560168 | MsG0180005075.01.T01:CDS | 55.0% |
| !! | CGGTTTGTTGAAGCGTCAGG+AGG | - | Chr1:86560152-86560171 | MsG0180005075.01.T01:CDS | 55.0% |
| AAGCGTCAGGAGGTCTGTCG+AGG | - | Chr1:86560162-86560181 | MsG0180005075.01.T01:CDS | 60.0% | |
| AGCGTCAGGAGGTCTGTCGA+GGG | - | Chr1:86560163-86560182 | MsG0180005075.01.T01:CDS | 60.0% | |
| ! | AGGAGGTCTGTCGAGGGTGT+GGG | - | Chr1:86560169-86560188 | MsG0180005075.01.T01:CDS | 60.0% |
| ! | GGAGTGTTAGGGGGTTGGGT+GGG | - | Chr1:86559985-86560004 | MsG0180005075.01.T01:CDS | 60.0% |
| ! | TGGAGTGTTAGGGGGTTGGG+TGG | - | Chr1:86559984-86560003 | MsG0180005075.01.T01:CDS | 60.0% |
| CAGGAGGTCTGTCGAGGGTG+TGG | - | Chr1:86560168-86560187 | MsG0180005075.01.T01:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr1 | gene | 86559969 | 86560325 | 86559969 | ID=MsG0180005075.01;Name=MsG0180005075.01 |
| Chr1 | mRNA | 86559969 | 86560325 | 86559969 | ID=MsG0180005075.01.T01;Parent=MsG0180005075.01;Name=MsG0180005075.01.T01;_AED=0.41;_eAED=0.41;_QI=0|-1|0|1|-1|1|1|0|118 |
| Chr1 | exon | 86559969 | 86560325 | 86559969 | ID=MsG0180005075.01.T01:exon:10040;Parent=MsG0180005075.01.T01 |
| Chr1 | CDS | 86559969 | 86560325 | 86559969 | ID=MsG0180005075.01.T01:cds;Parent=MsG0180005075.01.T01 |
| Gene Sequence |
| Protein sequence |
