AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0180005078.01


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180005078.01.T01 MTR_3g437790 83.152 184 25 3 10 187 3 186 4.07e-99 284
MsG0180005078.01.T01 MTR_2g045020 61.081 185 64 6 1 181 1 181 9.29e-60 185
MsG0180005078.01.T01 MTR_8g022970 50.857 175 73 6 15 180 10 180 2.76e-46 150
MsG0180005078.01.T01 MTR_3g109930 51.656 151 69 3 31 179 16 164 1.62e-44 145
MsG0180005078.01.T01 MTR_2g105290 36.196 163 96 3 12 168 1 161 1.91e-24 94.4
MsG0180005078.01.T01 MTR_7g028448 38.060 134 71 3 36 168 31 153 7.96e-22 87.4
MsG0180005078.01.T01 MTR_4g036915 38.060 134 71 3 36 168 31 153 7.96e-22 87.4
MsG0180005078.01.T01 MTR_8g086290 35.065 154 92 3 18 165 7 158 1.34e-20 84.3
MsG0180005078.01.T01 MTR_5g053390 36.774 155 86 4 21 165 7 159 3.02e-18 79.7
MsG0180005078.01.T01 MTR_4g127140 33.803 142 84 3 36 168 31 171 9.00e-17 74.7
MsG0180005078.01.T01 MTR_8g043650 30.909 165 95 4 14 165 1 159 5.08e-16 73.6
MsG0180005078.01.T01 MTR_1g012570 32.298 161 85 5 21 165 7 159 1.13e-15 72.8
MsG0180005078.01.T01 MTR_3g052920 32.278 158 89 3 21 165 7 159 1.20e-15 72.8
MsG0180005078.01.T01 MTR_1g105920 31.646 158 90 4 21 165 7 159 1.67e-15 72.4
MsG0180005078.01.T01 MTR_1g106070 31.013 158 91 3 21 165 7 159 2.36e-15 70.9
MsG0180005078.01.T01 MTR_3g030770 33.125 160 83 5 22 165 8 159 2.95e-15 71.6
MsG0180005078.01.T01 MTR_1g105910 31.013 158 91 3 21 165 7 159 6.52e-15 70.9
MsG0180005078.01.T01 MTR_2g085280 29.375 160 91 4 21 165 7 159 1.42e-14 69.7
MsG0180005078.01.T01 MTR_2g085250 29.375 160 91 4 21 165 7 159 1.42e-14 69.7
MsG0180005078.01.T01 MTR_3g080940 29.861 144 83 3 36 169 28 163 1.99e-14 68.9
MsG0180005078.01.T01 MTR_2g030740 32.298 161 85 5 21 165 7 159 2.11e-14 69.3
MsG0180005078.01.T01 MTR_1g108510 32.500 160 84 5 22 165 8 159 2.22e-14 68.9
MsG0180005078.01.T01 MTR_1g108580 32.500 160 84 5 22 165 8 159 8.74e-14 67.4
MsG0180005078.01.T01 MTR_5g055100 32.895 152 76 6 21 155 7 149 4.27e-13 65.9
MsG0180005078.01.T01 MTR_8g046350 41.096 73 37 1 24 90 3 75 1.35e-12 63.2
MsG0180005078.01.T01 MTR_1g047550 35.577 104 59 2 24 119 3 106 2.59e-12 62.8
MsG0180005078.01.T01 MTR_8g079502 31.818 110 67 2 24 125 3 112 8.03e-12 61.2
MsG0180005078.01.T01 MTR_3g052870 43.056 72 35 1 21 86 7 78 1.05e-11 61.6
MsG0180005078.01.T01 MTR_0121s0080 33.824 136 78 4 37 165 29 159 1.09e-11 62.0
MsG0180005078.01.T01 MTR_1g105905 30.216 139 79 4 36 164 11 141 1.26e-11 61.6
MsG0180005078.01.T01 MTR_2g093190 28.931 159 91 3 22 165 8 159 2.29e-11 60.8
MsG0180005078.01.T01 MTR_8g051580 46.552 58 31 0 36 93 21 78 2.38e-11 60.1
MsG0180005078.01.T01 MTR_1g077360 29.193 161 90 3 21 165 7 159 2.98e-11 60.8
MsG0180005078.01.T01 MTR_7g062350 33.577 137 79 4 36 165 40 171 5.61e-11 60.1
MsG0180005078.01.T01 MTR_1g063160 42.647 68 33 1 24 85 3 70 7.54e-11 58.5
MsG0180005078.01.T02 MTR_3g437790 82.778 180 25 3 1 174 7 186 3.51e-96 276
MsG0180005078.01.T02 MTR_2g045020 63.473 167 53 6 6 168 19 181 2.92e-55 173
MsG0180005078.01.T02 MTR_8g022970 50.857 175 73 6 2 167 10 180 2.05e-46 150
MsG0180005078.01.T02 MTR_3g109930 49.375 160 77 3 9 166 7 164 1.00e-44 145
MsG0180005078.01.T02 MTR_2g105290 36.646 161 94 3 1 155 3 161 1.19e-24 94.7
MsG0180005078.01.T02 MTR_7g028448 38.060 134 71 3 23 155 31 153 4.73e-22 87.8
MsG0180005078.01.T02 MTR_4g036915 38.060 134 71 3 23 155 31 153 4.73e-22 87.8
MsG0180005078.01.T02 MTR_8g086290 35.065 154 92 3 5 152 7 158 1.04e-20 84.3
MsG0180005078.01.T02 MTR_5g053390 36.774 155 86 4 8 152 7 159 2.28e-18 79.7
MsG0180005078.01.T02 MTR_4g127140 33.803 142 84 3 23 155 31 171 6.05e-17 74.7
MsG0180005078.01.T02 MTR_8g043650 30.909 165 95 4 1 152 1 159 3.75e-16 73.6
MsG0180005078.01.T02 MTR_3g052920 32.121 165 93 4 1 152 1 159 8.82e-16 72.8
MsG0180005078.01.T02 MTR_1g012570 32.298 161 85 5 8 152 7 159 1.04e-15 72.4
MsG0180005078.01.T02 MTR_1g106070 30.909 165 95 4 1 152 1 159 1.48e-15 70.9
MsG0180005078.01.T02 MTR_1g105920 31.646 158 90 4 8 152 7 159 1.54e-15 72.0
MsG0180005078.01.T02 MTR_3g030770 33.125 160 83 5 9 152 8 159 1.92e-15 71.6
MsG0180005078.01.T02 MTR_1g105910 31.013 158 91 3 8 152 7 159 4.93e-15 70.5
MsG0180005078.01.T02 MTR_2g085250 29.375 160 91 4 8 152 7 159 8.93e-15 70.1
MsG0180005078.01.T02 MTR_2g085280 29.375 160 91 4 8 152 7 159 9.21e-15 70.1
MsG0180005078.01.T02 MTR_3g080940 29.861 144 83 3 23 156 28 163 1.56e-14 68.9
MsG0180005078.01.T02 MTR_1g108510 32.500 160 84 5 9 152 8 159 1.76e-14 68.9
MsG0180005078.01.T02 MTR_2g030740 32.298 161 85 5 8 152 7 159 2.25e-14 68.9
MsG0180005078.01.T02 MTR_1g108580 32.500 160 84 5 9 152 8 159 6.88e-14 67.4
MsG0180005078.01.T02 MTR_5g055100 32.895 152 76 6 8 142 7 149 3.12e-13 65.9
MsG0180005078.01.T02 MTR_8g046350 41.096 73 37 1 11 77 3 75 9.33e-13 63.2
MsG0180005078.01.T02 MTR_1g047550 37.209 86 52 1 23 106 21 106 1.75e-12 62.8
MsG0180005078.01.T02 MTR_8g079502 31.532 111 68 2 11 113 3 113 5.46e-12 61.6
MsG0180005078.01.T02 MTR_0121s0080 33.824 136 78 4 24 152 29 159 6.38e-12 62.0
MsG0180005078.01.T02 MTR_3g052870 43.056 72 35 1 8 73 7 78 8.18e-12 61.6
MsG0180005078.01.T02 MTR_1g105905 30.216 139 79 4 23 151 11 141 9.67e-12 61.6
MsG0180005078.01.T02 MTR_2g093190 30.573 157 91 4 9 152 8 159 1.37e-11 61.2
MsG0180005078.01.T02 MTR_8g051580 46.552 58 31 0 23 80 21 78 1.66e-11 60.1
MsG0180005078.01.T02 MTR_1g077360 29.193 161 90 3 8 152 7 159 2.25e-11 60.8
MsG0180005078.01.T02 MTR_7g062350 33.577 137 79 4 23 152 40 171 3.11e-11 60.5
MsG0180005078.01.T02 MTR_1g063160 42.647 68 33 1 11 72 3 70 5.94e-11 58.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180005078.01.T01 AT2G24840 28.090 178 106 5 8 175 63 228 7.87e-16 73.6
MsG0180005078.01.T01 AT5G60440 31.081 148 93 4 36 177 26 170 8.60e-16 73.9
MsG0180005078.01.T01 AT3G66656 53.061 49 23 0 36 84 21 69 7.88e-13 63.9
MsG0180005078.01.T01 AT1G65360 30.345 145 82 5 21 152 5 143 4.22e-12 62.8
MsG0180005078.01.T01 AT4G36590 28.571 161 97 5 21 165 5 163 1.91e-11 61.2
MsG0180005078.01.T01 AT2G34440 29.508 122 80 2 36 154 21 139 3.59e-11 59.3
MsG0180005078.01.T02 AT5G60440 31.081 148 93 4 23 164 26 170 5.18e-16 74.3
MsG0180005078.01.T02 AT2G24840 29.299 157 101 4 16 162 72 228 1.43e-15 72.4
MsG0180005078.01.T02 AT3G66656 53.061 49 23 0 23 71 21 69 6.45e-13 63.9
MsG0180005078.01.T02 AT1G65360 30.345 145 82 5 8 139 5 143 3.71e-12 62.8
MsG0180005078.01.T02 AT4G36590 28.571 161 97 5 8 152 5 163 1.23e-11 61.6
MsG0180005078.01.T02 AT2G34440 29.508 122 80 2 23 141 21 139 2.88e-11 59.3

Find 25 sgRNAs with CRISPR-Local

Find 30 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TAACACACATTCTCTTGAAT+TGG 0.284587 1:+86587800 None:intergenic
GAATAAATCTATTGATGATA+TGG 0.314688 1:-86587915 MsG0180005078.01.T01:CDS
TGAATGACAACGTCATAGTT+AGG 0.360102 1:+86588118 None:intergenic
TCGCTTCATATCGAAGCCTT+AGG 0.376463 1:+86588026 None:intergenic
TTGTTTCTCTCGGCCGCCAT+TGG 0.404729 1:+86588304 None:intergenic
CTTCCGTGTGTTGTTTCTCT+CGG 0.413838 1:+86588294 None:intergenic
CCCTTCTCGAAACTCAAACT+TGG 0.418484 1:-86588238 MsG0180005078.01.T01:CDS
CGCTTCATATCGAAGCCTTA+GGG 0.430275 1:+86588027 None:intergenic
CTCATCCTCGTCCATATGTC+CGG 0.445428 1:+86588084 None:intergenic
CTTCTTCGCTTCGCTTGCTA+CGG 0.451335 1:+86587835 None:intergenic
GAAGGATGAAATCAAGAAAG+AGG 0.477445 1:-86588266 MsG0180005078.01.T01:CDS
GATTTATTCCACCAATCAGC+AGG 0.485193 1:+86587929 None:intergenic
CTTCGATATGAAGCGATTCA+AGG 0.502715 1:-86588019 MsG0180005078.01.T01:CDS
CATGCATAGAACTTGTTGTT+GGG 0.522753 1:+86588148 None:intergenic
AGGAAAACTTAAAGAAGAGA+AGG 0.524858 1:-86587999 MsG0180005078.01.T01:CDS
CAACACACGGAAGAGAAAGA+AGG 0.527351 1:-86588284 MsG0180005078.01.T01:CDS
CCAAGTTTGAGTTTCGAGAA+GGG 0.530556 1:+86588238 None:intergenic
GAATGACAACGTCATAGTTA+GGG 0.531829 1:+86588119 None:intergenic
TTGATTTGCCTGCTGATTGG+TGG 0.538450 1:-86587937 MsG0180005078.01.T01:CDS
TCCAAGTTTGAGTTTCGAGA+AGG 0.565563 1:+86588237 None:intergenic
ATGATGTTGTTGAAACCCTA+AGG 0.589934 1:-86588042 MsG0180005078.01.T01:CDS
ACATGCATAGAACTTGTTGT+TGG 0.594378 1:+86588147 None:intergenic
AACAACAAGTTCTATGCATG+TGG 0.619639 1:-86588145 MsG0180005078.01.T01:CDS
CGGCCGAGAGAAACAACACA+CGG 0.685523 1:-86588297 MsG0180005078.01.T01:CDS
TCTGACCGGACATATGGACG+AGG 0.696130 1:-86588089 MsG0180005078.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! GAATAAATCTATTGATGATA+TGG - Chr1:86588224-86588243 MsG0180005078.01.T01:CDS 20.0%
! GTCTAAGAAAAAAATTCCAA+TGG - Chr1:86587819-86587838 MsG0180005078.01.T01:CDS 25.0%
! TAAGAAAAAAATTCCAATGG+CGG - Chr1:86587822-86587841 MsG0180005078.01.T01:CDS 25.0%
AGGAAAACTTAAAGAAGAGA+AGG - Chr1:86588140-86588159 MsG0180005078.01.T01:CDS 30.0%
TAACACACATTCTCTTGAAT+TGG + Chr1:86588342-86588361 None:intergenic 30.0%
AACAACAAGTTCTATGCATG+TGG - Chr1:86587994-86588013 MsG0180005078.01.T01:CDS 35.0%
ATGATGTTGTTGAAACCCTA+AGG - Chr1:86588097-86588116 MsG0180005078.01.T01:CDS 35.0%
GAAGGATGAAATCAAGAAAG+AGG - Chr1:86587873-86587892 MsG0180005078.01.T01:CDS 35.0%
GAATGACAACGTCATAGTTA+GGG + Chr1:86588023-86588042 None:intergenic 35.0%
TGAATGACAACGTCATAGTT+AGG + Chr1:86588024-86588043 None:intergenic 35.0%
!! ACATGCATAGAACTTGTTGT+TGG + Chr1:86587995-86588014 None:intergenic 35.0%
!! CATGCATAGAACTTGTTGTT+GGG + Chr1:86587994-86588013 None:intergenic 35.0%
!!! ATTTTGATTTGCCTGCTGAT+TGG - Chr1:86588199-86588218 MsG0180005078.01.T01:CDS 35.0%
CTTCGATATGAAGCGATTCA+AGG - Chr1:86588120-86588139 MsG0180005078.01.T01:CDS 40.0%
GATTTATTCCACCAATCAGC+AGG + Chr1:86588213-86588232 None:intergenic 40.0%
! CCAAGTTTGAGTTTCGAGAA+GGG + Chr1:86587904-86587923 None:intergenic 40.0%
! TCCAAGTTTGAGTTTCGAGA+AGG + Chr1:86587905-86587924 None:intergenic 40.0%
CAACACACGGAAGAGAAAGA+AGG - Chr1:86587855-86587874 MsG0180005078.01.T01:CDS 45.0%
CCCTTCTCGAAACTCAAACT+TGG - Chr1:86587901-86587920 MsG0180005078.01.T01:CDS 45.0%
CGCTTCATATCGAAGCCTTA+GGG + Chr1:86588115-86588134 None:intergenic 45.0%
TCGCTTCATATCGAAGCCTT+AGG + Chr1:86588116-86588135 None:intergenic 45.0%
! GTCATTCAGCGTTTTCTGAC+CGG - Chr1:86588036-86588055 MsG0180005078.01.T01:CDS 45.0%
!! CTTCCGTGTGTTGTTTCTCT+CGG + Chr1:86587848-86587867 None:intergenic 45.0%
!! TTGATTTGCCTGCTGATTGG+TGG - Chr1:86588202-86588221 MsG0180005078.01.T01:CDS 45.0%
CTCATCCTCGTCCATATGTC+CGG + Chr1:86588058-86588077 None:intergenic 50.0%
! CTTCTTCGCTTCGCTTGCTA+CGG + Chr1:86588307-86588326 None:intergenic 50.0%
! GCGTTTTCTGACCGGACATA+TGG - Chr1:86588044-86588063 MsG0180005078.01.T01:CDS 50.0%
CGGCCGAGAGAAACAACACA+CGG - Chr1:86587842-86587861 MsG0180005078.01.T01:CDS 55.0%
TCTGACCGGACATATGGACG+AGG - Chr1:86588050-86588069 MsG0180005078.01.T01:CDS 55.0%
! TTGTTTCTCTCGGCCGCCAT+TGG + Chr1:86587838-86587857 None:intergenic 55.0%
Chromosome Type Strat End Strand Name
Chr1 gene 86587799 86588362 86587799 ID=MsG0180005078.01;Name=MsG0180005078.01
Chr1 mRNA 86587799 86588362 86587799 ID=MsG0180005078.01.T01;Parent=MsG0180005078.01;Name=MsG0180005078.01.T01;_AED=0.00;_eAED=0.00;_QI=0|-1|0|1|-1|0|1|0|187
Chr1 exon 86587799 86588362 86587799 ID=MsG0180005078.01.T01:exon:10104;Parent=MsG0180005078.01.T01
Chr1 CDS 86587799 86588362 86587799 ID=MsG0180005078.01.T01:cds;Parent=MsG0180005078.01.T01
Chr1 mRNA 86587799 86588323 86587799 ID=MsG0180005078.01.T02;Parent=MsG0180005078.01;Name=MsG0180005078.01.T02;_AED=0.04;_eAED=0.04;_QI=0|-1|0|1|-1|0|1|0|174
Chr1 exon 86587799 86588323 86587799 ID=MsG0180005078.01.T02:exon:10105;Parent=MsG0180005078.01.T02
Chr1 CDS 86587799 86588323 86587799 ID=MsG0180005078.01.T02:cds;Parent=MsG0180005078.01.T02
Chr1 mRNA 86588136 86588204 86588136 ID=MsG0180005078.01.T03;Parent=MsG0180005078.01;Name=MsG0180005078.01.T03;_AED=0.44;_eAED=1.00;_QI=0|-1|0|1|-1|0|1|0|22
Chr1 exon 86588136 86588204 86588136 ID=MsG0180005078.01.T03:exon:10106;Parent=MsG0180005078.01.T03
Chr1 CDS 86588136 86588204 86588136 ID=MsG0180005078.01.T03:cds;Parent=MsG0180005078.01.T03
Gene Sequence

>MsG0180005078.01.T03

ATGCGAATCAGAAACTGCTCTCATCGTAACATCACCCAACAACAAGTTCTATGCATGTGGCTACCCTAA

>MsG0180005078.01.T01

ATGAATAAGAATTGTGAAACGTCTAAGAAAAAAATTCCAATGGCGGCCGAGAGAAACAACACACGGAAGAGAAAGAAGGATGAAATCAAGAAAGAGGAAAAGCCCTTCTCGAAACTCAAACTTGGAATTTTCAACAAAGCAATAGAGCTATCTATTCTATGCGAATCAGAAACTGCTCTCATCGTAACATCACCCAACAACAAGTTCTATGCATGTGGCTACCCTAACTATGACGTTGTCATTCAGCGTTTTCTGACCGGACATATGGACGAGGATGAGAAAAATAAGCAAGAAGATGATGATGTTGTTGAAACCCTAAGGCTTCGATATGAAGCGATTCAAGGAAAACTTAAAGAAGAGAAGGAGAAATTGCAAGCTGTCAAAGATGCACAAAAGAGTAATTTTGATTTGCCTGCTGATTGGTGGAATAAATCTATTGATGATATGGATTTAACATCTCTTGAAGATATTACGACTTCGTTGAATAAATTGAAGATTAATTTGTCCGTAGCAAGCGAAGCGAAGAAGTTCAATTTACATCCAATTCAAGAGAATGTGTGTTAA

>MsG0180005078.01.T02

ATGGCGGCCGAGAGAAACAACACACGGAAGAGAAAGAAGGATGAAATCAAGAAAGAGGAAAAGCCCTTCTCGAAACTCAAACTTGGAATTTTCAACAAAGCAATAGAGCTATCTATTCTATGCGAATCAGAAACTGCTCTCATCGTAACATCACCCAACAACAAGTTCTATGCATGTGGCTACCCTAACTATGACGTTGTCATTCAGCGTTTTCTGACCGGACATATGGACGAGGATGAGAAAAATAAGCAAGAAGATGATGATGTTGTTGAAACCCTAAGGCTTCGATATGAAGCGATTCAAGGAAAACTTAAAGAAGAGAAGGAGAAATTGCAAGCTGTCAAAGATGCACAAAAGAGTAATTTTGATTTGCCTGCTGATTGGTGGAATAAATCTATTGATGATATGGATTTAACATCTCTTGAAGATATTACGACTTCGTTGAATAAATTGAAGATTAATTTGTCCGTAGCAAGCGAAGCGAAGAAGTTCAATTTACATCCAATTCAAGAGAATGTGTGTTAA

Protein sequence

>MsG0180005078.01.T03

MRIRNCSHRNITQQQVLCMWLP*

>MsG0180005078.01.T01

MNKNCETSKKKIPMAAERNNTRKRKKDEIKKEEKPFSKLKLGIFNKAIELSILCESETALIVTSPNNKFYACGYPNYDVVIQRFLTGHMDEDEKNKQEDDDVVETLRLRYEAIQGKLKEEKEKLQAVKDAQKSNFDLPADWWNKSIDDMDLTSLEDITTSLNKLKINLSVASEAKKFNLHPIQENVC*

>MsG0180005078.01.T02

MAAERNNTRKRKKDEIKKEEKPFSKLKLGIFNKAIELSILCESETALIVTSPNNKFYACGYPNYDVVIQRFLTGHMDEDEKNKQEDDDVVETLRLRYEAIQGKLKEEKEKLQAVKDAQKSNFDLPADWWNKSIDDMDLTSLEDITTSLNKLKINLSVASEAKKFNLHPIQENVC*