AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0180005329.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180005329.01.T01 MTR_1g097250 97.778 360 8 0 1 360 1 360 0.0 734
MsG0180005329.01.T01 MTR_6g038640 69.014 355 110 0 6 360 7 361 0.0 531
MsG0180005329.01.T01 MTR_6g038650 66.479 355 114 2 6 360 7 356 3.33e-179 501
MsG0180005329.01.T01 MTR_6g038390 61.582 354 136 0 6 359 9 362 6.46e-165 465
MsG0180005329.01.T01 MTR_6g038350 62.994 354 131 0 6 359 9 362 1.76e-158 449
MsG0180005329.01.T01 MTR_6g038370 62.994 354 131 0 6 359 9 362 1.76e-158 449
MsG0180005329.01.T01 MTR_6g066230 52.632 361 166 2 2 358 3 362 4.07e-139 400
MsG0180005329.01.T01 MTR_3g064900 49.020 357 178 2 6 358 7 363 3.53e-122 357
MsG0180005329.01.T01 MTR_6g038310 66.972 109 36 0 6 114 9 117 2.70e-40 139
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180005329.01.T01 AT4G09670 65.266 357 124 0 2 358 3 359 1.66e-177 497
MsG0180005329.01.T01 AT1G34200 58.523 352 134 3 6 356 7 347 3.52e-143 410
MsG0180005329.01.T01 AT1G66130 38.950 362 208 6 6 358 7 364 4.34e-83 257

Find 102 sgRNAs with CRISPR-Local

Find 235 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CTCTGCTCAATTGGCATCTT+TGG 0.113457 1:-90397658 MsG0180005329.01.T01:three_prime_UTR
GGATAATGGGGTTCAGTTTA+TGG 0.156726 1:-90399827 MsG0180005329.01.T01:CDS
GAAGCAAATGCTTCTTATTC+TGG 0.175761 1:+90399908 None:intergenic
AACATTCCATTGTTCTTTCT+TGG 0.207423 1:-90398082 MsG0180005329.01.T01:CDS
TAACAAAGATGGAGTTATAT+TGG 0.254546 1:-90398145 MsG0180005329.01.T01:CDS
GATCAGTGCTTCTGACATTT+TGG 0.256227 1:+90400185 None:intergenic
TCTTCCCCAGGAGGCTCTTT+TGG 0.258830 1:-90397884 MsG0180005329.01.T01:CDS
TAGGGGCTTGTGAGGATAAT+GGG 0.271562 1:-90399840 MsG0180005329.01.T01:CDS
TAATCCTAGGACTGCTAAAA+TGG 0.281705 1:-90399785 MsG0180005329.01.T01:CDS
CGGCGCAGATGGTGGCGTTT+GGG 0.299088 1:+90400100 None:intergenic
ACGGCGCAGATGGTGGCGTT+TGG 0.303690 1:+90400099 None:intergenic
TTTATATTATATTGAGAGAT+TGG 0.310543 1:-90400263 MsG0180005329.01.T01:five_prime_UTR
CAAAATGTGCTCTGCTCAAT+TGG 0.320258 1:-90397667 MsG0180005329.01.T01:three_prime_UTR
GTAGGGGCTTGTGAGGATAA+TGG 0.330818 1:-90399841 MsG0180005329.01.T01:CDS
CTGTTAAGTCTTCAATTCAT+TGG 0.331420 1:-90397759 MsG0180005329.01.T01:CDS
TGTTAAGTCTTCAATTCATT+GGG 0.336926 1:-90397758 MsG0180005329.01.T01:CDS
TTCAATGATCCGCACCGTTT+TGG 0.337672 1:-90399754 MsG0180005329.01.T01:CDS
ATTGTGGAGCATCTCTATAT+TGG 0.345525 1:-90398122 MsG0180005329.01.T01:CDS
GGTTACAGAATTTGCTCGTT+TGG 0.362448 1:-90397863 MsG0180005329.01.T01:CDS
ACAATGGATATAACAGCTAT+TGG 0.368508 1:-90398051 MsG0180005329.01.T01:CDS
TTGATGACCTTGTTACTTGT+TGG 0.374189 1:-90397942 MsG0180005329.01.T01:CDS
GATGCTTCATTCTCTTCATA+AGG 0.382468 1:+90397991 None:intergenic
ATGTATTTGTGAGCTGTTCA+TGG 0.385427 1:-90397589 MsG0180005329.01.T01:three_prime_UTR
GGCGTTTGGGGATAAGTTGA+TGG 0.387384 1:+90400113 None:intergenic
CACTTAAGATGCAAGCTTGT+TGG 0.390766 1:+90399946 None:intergenic
CGGGAACTACGAAGCTCTAC+TGG 0.398728 1:-90400004 MsG0180005329.01.T01:CDS
CACAGCCTACTACTCCGATT+TGG 0.398824 1:+90400163 None:intergenic
GTCGTAGTTGCGGCTGGCGA+CGG 0.414698 1:+90400080 None:intergenic
GGCTTTGTCGTAGTTGCGGC+TGG 0.417845 1:+90400074 None:intergenic
TTGTGGAGCATCTCTATATT+GGG 0.417986 1:-90398121 MsG0180005329.01.T01:CDS
TGATGACCTTGTTACTTGTT+GGG 0.419811 1:-90397941 MsG0180005329.01.T01:CDS
ACCGCTTTCGCCGCCGCCAA+TGG 0.424701 1:-90400051 MsG0180005329.01.T01:CDS
AAGCAAATGCTTCTTATTCT+GGG 0.425925 1:+90399909 None:intergenic
GAGCCATGTGCGTCAAGATC+TGG 0.426777 1:+90398264 None:intergenic
CCGTACACCTTCGTTGTTAG+AGG 0.427206 1:+90400024 None:intergenic
ACAATTGGCCACTTCTTCTC+AGG 0.432874 1:+90397811 None:intergenic
GCTGAGTTTGATGAAATTGT+AGG 0.435047 1:-90399859 MsG0180005329.01.T01:CDS
TCAGCAATATCCAGCGCAAC+AGG 0.438099 1:+90399877 None:intergenic
ATAACAGCTATTGGAACAAA+AGG 0.438590 1:-90398042 MsG0180005329.01.T01:CDS
ATTCACTCCTCAATCTGAAC+AGG 0.444248 1:+90397727 None:intergenic
CTTGACGCACATGGCTCTCT+AGG 0.464362 1:-90398258 MsG0180005329.01.T01:CDS
ATATACACAGCGTCCACATC+AGG 0.464643 1:+90399976 None:intergenic
CTGATCCAAATCGGAGTAGT+AGG 0.471545 1:-90400168 MsG0180005329.01.T01:CDS
TCTTGAGAAGCCTGTTGCGC+TGG 0.474410 1:-90399887 MsG0180005329.01.T01:CDS
AGATGCAAGCTTGTTGGTAG+TGG 0.477549 1:+90399952 None:intergenic
AGATAGAGAGTGAGTGAGTG+TGG 0.479328 1:+90400222 None:intergenic
AGAAGTGGCCAATTGTTAGT+AGG 0.484377 1:-90397804 MsG0180005329.01.T01:CDS
ACTCCATCTTTGTTAAGCAC+TGG 0.485256 1:+90398153 None:intergenic
GCGGCTGGCGACGGCGCAGA+TGG 0.488870 1:+90400089 None:intergenic
GCCATTGGCGGCGGCGAAAG+CGG 0.493499 1:+90400050 None:intergenic
CTGTAACCAAAAGAGCCTCC+TGG 0.493809 1:+90397878 None:intergenic
CAACAAGCTTGCATCTTAAG+TGG 0.494377 1:-90399945 MsG0180005329.01.T01:CDS
ATCTGCAGCAAATGTAAAGC+AGG 0.496037 1:+90398320 None:intergenic
CTGAGTTTGATGAAATTGTA+GGG 0.498944 1:-90399858 MsG0180005329.01.T01:CDS
CTATGTGGGTTCATAATCCT+AGG 0.500670 1:-90399798 MsG0180005329.01.T01:CDS
TGTAACCAAAAGAGCCTCCT+GGG 0.506711 1:+90397879 None:intergenic
TACAGAATTTGCTCGTTTGG+TGG 0.508036 1:-90397860 MsG0180005329.01.T01:CDS
GCTGGCGACGGCGCAGATGG+TGG 0.508895 1:+90400092 None:intergenic
GCATCTCTATATTGGGAAGA+TGG 0.509643 1:-90398114 MsG0180005329.01.T01:CDS
CTCTAACAACGAAGGTGTAC+GGG 0.512039 1:-90400023 MsG0180005329.01.T01:CDS
TCAGAAGCACTGATCCAAAT+CGG 0.512386 1:-90400177 MsG0180005329.01.T01:CDS
CATGGCTCTCTAGGTGATGC+AGG 0.514346 1:-90398249 MsG0180005329.01.T01:CDS
CCGATTTGAGTTGACCAAAA+CGG 0.515121 1:+90399740 None:intergenic
CCTCTAACAACGAAGGTGTA+CGG 0.518517 1:-90400024 MsG0180005329.01.T01:CDS
TGTTAGAGGGTAGCCATTGG+CGG 0.524038 1:+90400038 None:intergenic
ACAGAATTTGCTCGTTTGGT+GGG 0.526034 1:-90397859 MsG0180005329.01.T01:CDS
GGCGCAGATGGTGGCGTTTG+GGG 0.530549 1:+90400101 None:intergenic
GAGTGTGGTATTTACTTGGT+CGG 0.531473 1:+90400237 None:intergenic
ATTGGCGGCGGCGAAAGCGG+TGG 0.533305 1:+90400053 None:intergenic
GTTTATGGATAATACTATGT+GGG 0.534341 1:-90399812 MsG0180005329.01.T01:CDS
CGTTGTTAGAGGGTAGCCAT+TGG 0.535437 1:+90400035 None:intergenic
TCTTAAGTGGGCCGTTCTTG+CGG 0.537152 1:-90399932 MsG0180005329.01.T01:CDS
CTTATTCTGGGCCGCAAGAA+CGG 0.540934 1:+90399921 None:intergenic
GTTCGTAGTTGGCAGCTAAG+AGG 0.542085 1:+90398205 None:intergenic
TAGAGGGTAGCCATTGGCGG+CGG 0.544680 1:+90400041 None:intergenic
TGTTAAAACTGTTCTTCCCC+AGG 0.549508 1:-90397896 MsG0180005329.01.T01:CDS
AGAAACTTTACGAGCTATGT+CGG 0.550870 1:+90400140 None:intergenic
GCTCTCTAGGTGATGCAGGT+TGG 0.554687 1:-90398245 MsG0180005329.01.T01:CDS
TTTCTTGGCAAACTTGACAA+TGG 0.557338 1:-90398067 MsG0180005329.01.T01:CDS
GATGGAGTTATATTGGATTG+TGG 0.557852 1:-90398138 MsG0180005329.01.T01:CDS
AGTTTATGGATAATACTATG+TGG 0.557985 1:-90399813 MsG0180005329.01.T01:CDS
ACTCTAAGCCTGAGAAGAAG+TGG 0.559686 1:-90397819 MsG0180005329.01.T01:CDS
CGTACACCTTCGTTGTTAGA+GGG 0.561415 1:+90400025 None:intergenic
CGGTGGCTTTGTCGTAGTTG+CGG 0.562152 1:+90400070 None:intergenic
GTTAAGTCTTCAATTCATTG+GGG 0.569780 1:-90397757 MsG0180005329.01.T01:CDS
GAGTGAGTGTGGTATTTACT+TGG 0.570233 1:+90400233 None:intergenic
GAACCAGTGCTTAACAAAGA+TGG 0.577418 1:-90398156 MsG0180005329.01.T01:CDS
TCAGATTGAGGAGTGAATCA+AGG 0.584517 1:-90397722 MsG0180005329.01.T01:exon
AGGGGCTTGTGAGGATAATG+GGG 0.588028 1:-90399839 MsG0180005329.01.T01:CDS
AACAAGCTTGCATCTTAAGT+GGG 0.603161 1:-90399944 MsG0180005329.01.T01:CDS
AAGCCAGATCTTGACGCACA+TGG 0.604386 1:-90398267 MsG0180005329.01.T01:CDS
GTTAAGCACTGGTTCACGTG+AGG 0.613013 1:+90398164 None:intergenic
TGAAATTGTAGGGGCTTGTG+AGG 0.623398 1:-90399848 MsG0180005329.01.T01:CDS
GTTTAGCCCAACAAGTAACA+AGG 0.658553 1:+90397935 None:intergenic
TATACACAGCGTCCACATCA+GGG 0.659580 1:+90399977 None:intergenic
AGTTTGCCAAGAAAGAACAA+TGG 0.672798 1:+90398076 None:intergenic
TAAAACTGTTCTTCCCCAGG+AGG 0.677428 1:-90397893 MsG0180005329.01.T01:CDS
TGAGTTTGATGAAATTGTAG+GGG 0.678209 1:-90399857 MsG0180005329.01.T01:CDS
TGGCTACCCTCTAACAACGA+AGG 0.692150 1:-90400031 MsG0180005329.01.T01:CDS
TCTACTGGAAGACCCTGATG+TGG 0.699995 1:-90399989 MsG0180005329.01.T01:CDS
GTAACCAAAAGAGCCTCCTG+GGG 0.700455 1:+90397880 None:intergenic
TTGAGTTGACCAAAACGGTG+CGG 0.715606 1:+90399745 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! TTTGAAATATTTGTAAAATT+TGG - Chr1:90399359-90399378 MsG0180005329.01.T01:intron 10.0%
!!! GATTTTGTTTATTTTTCTTT+TGG - Chr1:90398480-90398499 MsG0180005329.01.T01:intron 15.0%
!!! TAAAATGATATTAAGTTGTT+CGG - Chr1:90399204-90399223 MsG0180005329.01.T01:intron 15.0%
!! AAAAAAAATAGTGAAAACAG+AGG - Chr1:90398959-90398978 MsG0180005329.01.T01:intron 20.0%
!! ATAGTAGTAAATACTCAAAT+TGG - Chr1:90399067-90399086 MsG0180005329.01.T01:intron 20.0%
!! TGCTTATTACTATACAAAAA+TGG + Chr1:90398300-90398319 None:intergenic 20.0%
!!! ATCATTTAGAATATCGATAA+TGG + Chr1:90399138-90399157 None:intergenic 20.0%
!!! ATTTTTGTATAGTAATAAGC+AGG - Chr1:90398299-90398318 MsG0180005329.01.T01:CDS 20.0%
!!! TCACTATTTTTTTTTTTGCT+TGG + Chr1:90398953-90398972 None:intergenic 20.0%
!!! TCATTTAGAATATCGATAAT+GGG + Chr1:90399137-90399156 None:intergenic 20.0%
!!! TTTATTTTTCTTTTGGTGTA+TGG - Chr1:90398487-90398506 MsG0180005329.01.T01:intron 20.0%
!!! TTTTTGTATAGTAATAAGCA+GGG - Chr1:90398300-90398319 MsG0180005329.01.T01:CDS 20.0%
! AGTTTATGGATAATACTATG+TGG - Chr1:90397983-90398002 MsG0180005329.01.T01:CDS 25.0%
! CAAAGCTTAACAAATCTATT+TGG + Chr1:90398130-90398149 None:intergenic 25.0%
! CTGTTACAACAGATAAAATA+AGG + Chr1:90398595-90398614 None:intergenic 25.0%
! GTTTATGGATAATACTATGT+GGG - Chr1:90397984-90398003 MsG0180005329.01.T01:CDS 25.0%
! TAACAAAGATGGAGTTATAT+TGG - Chr1:90399651-90399670 MsG0180005329.01.T01:intron 25.0%
! TATCAATCTAGTATTCTAGT+TGG - Chr1:90399110-90399129 MsG0180005329.01.T01:intron 25.0%
! TATTTACTACTATTGTAGTC+AGG + Chr1:90399060-90399079 None:intergenic 25.0%
! TGACTATTTACTGATATAAG+TGG - Chr1:90399427-90399446 MsG0180005329.01.T01:intron 25.0%
! TGTTAAGTCTTCAATTCATT+GGG - Chr1:90400038-90400057 MsG0180005329.01.T01:CDS 25.0%
!! ATTTTCTCTATCTTTTTGCT+TGG + Chr1:90398912-90398931 None:intergenic 25.0%
!! TCCTATTTTACTTATTACCA+GGG + Chr1:90398764-90398783 None:intergenic 25.0%
!! TTCCTATTTTACTTATTACC+AGG + Chr1:90398765-90398784 None:intergenic 25.0%
!!! ACTAGGCTGTAAATATTTTT+TGG + Chr1:90398385-90398404 None:intergenic 25.0%
!!! GTTTTAACAGTACACTTAAT+TGG + Chr1:90399889-90399908 None:intergenic 25.0%
!!! TAATATCATTTTAGTCCCAA+CGG + Chr1:90399197-90399216 None:intergenic 25.0%
AACATTCCATTGTTCTTTCT+TGG - Chr1:90399714-90399733 MsG0180005329.01.T01:intron 30.0%
AAGCAAATGCTTCTTATTCT+GGG + Chr1:90397890-90397909 None:intergenic 30.0%
AAGTAAAATAGGAAGCAATG+TGG - Chr1:90398771-90398790 MsG0180005329.01.T01:intron 30.0%
ACAATGGATATAACAGCTAT+TGG - Chr1:90399745-90399764 MsG0180005329.01.T01:CDS 30.0%
ATAACAGCTATTGGAACAAA+AGG - Chr1:90399754-90399773 MsG0180005329.01.T01:CDS 30.0%
ATAGAGTTGAGAGAAAATAG+AGG - Chr1:90398873-90398892 MsG0180005329.01.T01:intron 30.0%
CTATTTACTGATATAAGTGG+TGG - Chr1:90399430-90399449 MsG0180005329.01.T01:intron 30.0%
CTGAGTTTGATGAAATTGTA+GGG - Chr1:90397938-90397957 MsG0180005329.01.T01:CDS 30.0%
CTGTTAAGTCTTCAATTCAT+TGG - Chr1:90400037-90400056 MsG0180005329.01.T01:CDS 30.0%
GTTAAGTCTTCAATTCATTG+GGG - Chr1:90400039-90400058 MsG0180005329.01.T01:CDS 30.0%
TAGAGTTGAGAGAAAATAGA+GGG - Chr1:90398874-90398893 MsG0180005329.01.T01:intron 30.0%
TGAGTTTGATGAAATTGTAG+GGG - Chr1:90397939-90397958 MsG0180005329.01.T01:CDS 30.0%
! ATTTTCACCACTAGACTTTT+TGG - Chr1:90399295-90399314 MsG0180005329.01.T01:intron 30.0%
! CTCATTCATACAATGTGTTT+TGG + Chr1:90398696-90398715 None:intergenic 30.0%
! TAAATACTCAAATTGGTTCC+TGG - Chr1:90399074-90399093 MsG0180005329.01.T01:intron 30.0%
! TGTGTTTTCCTACTAACAAT+TGG + Chr1:90400003-90400022 None:intergenic 30.0%
!! AATGAAGCATCTTTTCATGT+TGG - Chr1:90399817-90399836 MsG0180005329.01.T01:CDS 30.0%
!!! AGGCTGTAAATATTTTTTGG+AGG + Chr1:90398382-90398401 None:intergenic 30.0%
!!! ATACAATGTGTTTTGGAAAG+GGG + Chr1:90398689-90398708 None:intergenic 30.0%
!!! CATACAATGTGTTTTGGAAA+GGG + Chr1:90398690-90398709 None:intergenic 30.0%
!!! TCATACAATGTGTTTTGGAA+AGG + Chr1:90398691-90398710 None:intergenic 30.0%
AAAGCATGCTACAACAACAT+AGG + Chr1:90398432-90398451 None:intergenic 35.0%
AAAGGACACAGCAGAAAAAA+AGG + Chr1:90398539-90398558 None:intergenic 35.0%
AACAAGCTTGCATCTTAAGT+GGG - Chr1:90397852-90397871 MsG0180005329.01.T01:CDS 35.0%
ACTTTGTCCAAAAAGTCTAG+TGG + Chr1:90399305-90399324 None:intergenic 35.0%
AGAAAATAGAGGGAAAACAG+AGG - Chr1:90398884-90398903 MsG0180005329.01.T01:intron 35.0%
AGAAACTTTACGAGCTATGT+CGG + Chr1:90397659-90397678 None:intergenic 35.0%
AGTTTGCCAAGAAAGAACAA+TGG + Chr1:90399723-90399742 None:intergenic 35.0%
ATAGGAACTGGTATGTTAGT+GGG + Chr1:90398414-90398433 None:intergenic 35.0%
ATGTATTTGTGAGCTGTTCA+TGG - Chr1:90400207-90400226 MsG0180005329.01.T01:five_prime_UTR 35.0%
CAATGAAGAACGTAAACACA+AGG - Chr1:90398816-90398835 MsG0180005329.01.T01:intron 35.0%
CTTGTGTTTACGTTCTTCAT+TGG + Chr1:90398818-90398837 None:intergenic 35.0%
GAAGCAAATGCTTCTTATTC+TGG + Chr1:90397891-90397910 None:intergenic 35.0%
GACTGCAAAGTTTGATATGT+TGG - Chr1:90399250-90399269 MsG0180005329.01.T01:intron 35.0%
GATGCTTCATTCTCTTCATA+AGG + Chr1:90399808-90399827 None:intergenic 35.0%
GATGGAGTTATATTGGATTG+TGG - Chr1:90399658-90399677 MsG0180005329.01.T01:intron 35.0%
GCCCTGGTAATAAGTAAAAT+AGG - Chr1:90398760-90398779 MsG0180005329.01.T01:intron 35.0%
GCTGAGTTTGATGAAATTGT+AGG - Chr1:90397937-90397956 MsG0180005329.01.T01:CDS 35.0%
TAATCCTAGGACTGCTAAAA+TGG - Chr1:90398011-90398030 MsG0180005329.01.T01:CDS 35.0%
TACTATACCAAAGCAAACAG+CGG + Chr1:90398563-90398582 None:intergenic 35.0%
TCAAGACTACAGTATCATTG+AGG + Chr1:90398189-90398208 None:intergenic 35.0%
TGATATAGTCCTACAAATCC+AGG + Chr1:90399095-90399114 None:intergenic 35.0%
TTTCTTGGCAAACTTGACAA+TGG - Chr1:90399729-90399748 MsG0180005329.01.T01:intron 35.0%
! AAATTGGTTCCTGGATTTGT+AGG - Chr1:90399083-90399102 MsG0180005329.01.T01:intron 35.0%
! AATGAGCCTCATTTTACTTC+CGG - Chr1:90398710-90398729 MsG0180005329.01.T01:intron 35.0%
! AGTATCATTGAGGAAGTGTA+TGG + Chr1:90398179-90398198 None:intergenic 35.0%
! ATTGTGGAGCATCTCTATAT+TGG - Chr1:90399674-90399693 MsG0180005329.01.T01:intron 35.0%
! GTATCATTGAGGAAGTGTAT+GGG + Chr1:90398178-90398197 None:intergenic 35.0%
! TGATGACCTTGTTACTTGTT+GGG - Chr1:90399855-90399874 MsG0180005329.01.T01:CDS 35.0%
! TTGATGACCTTGTTACTTGT+TGG - Chr1:90399854-90399873 MsG0180005329.01.T01:CDS 35.0%
! TTGTGGAGCATCTCTATATT+GGG - Chr1:90399675-90399694 MsG0180005329.01.T01:intron 35.0%
!! AGCACATTTTGAGAAGATAC+AGG + Chr1:90400120-90400139 None:intergenic 35.0%
!! TCTTTTGGTGTATGGCTTTT+TGG - Chr1:90398495-90398514 MsG0180005329.01.T01:intron 35.0%
!!! TGTTGCTGTTTCGGTATTTT+GGG - Chr1:90398454-90398473 MsG0180005329.01.T01:intron 35.0%
AAATAGGAAGCAATGTGGTC+AGG - Chr1:90398776-90398795 MsG0180005329.01.T01:intron 40.0%
AAGGAACCATGTTCGTAAGT+AGG - Chr1:90398835-90398854 MsG0180005329.01.T01:intron 40.0%
ACCATGTTCGTAAGTAGGAT+CGG - Chr1:90398840-90398859 MsG0180005329.01.T01:intron 40.0%
ACTCCATCTTTGTTAAGCAC+TGG + Chr1:90399646-90399665 None:intergenic 40.0%
AGAAGTGGCCAATTGTTAGT+AGG - Chr1:90399992-90400011 MsG0180005329.01.T01:CDS 40.0%
ATCTGCAGCAAATGTAAAGC+AGG + Chr1:90399479-90399498 None:intergenic 40.0%
ATTCACTCCTCAATCTGAAC+AGG + Chr1:90400072-90400091 None:intergenic 40.0%
CAAAATGTGCTCTGCTCAAT+TGG - Chr1:90400129-90400148 MsG0180005329.01.T01:CDS 40.0%
CAACAAGCTTGCATCTTAAG+TGG - Chr1:90397851-90397870 MsG0180005329.01.T01:CDS 40.0%
CACTTAAGATGCAAGCTTGT+TGG + Chr1:90397853-90397872 None:intergenic 40.0%
CATAGGAACTGGTATGTTAG+TGG + Chr1:90398415-90398434 None:intergenic 40.0%
CCAAAAATAACAGCAGAGCT+AGG + Chr1:90398103-90398122 None:intergenic 40.0%
CTATGTGGGTTCATAATCCT+AGG - Chr1:90397998-90398017 MsG0180005329.01.T01:CDS 40.0%
CTGATATAAGTGGTGGCTTT+CGG - Chr1:90399437-90399456 MsG0180005329.01.T01:intron 40.0%
GAACCAGTGCTTAACAAAGA+TGG - Chr1:90399640-90399659 MsG0180005329.01.T01:intron 40.0%
GAGTGAGTGTGGTATTTACT+TGG + Chr1:90397566-90397585 None:intergenic 40.0%
GAGTGTGGTATTTACTTGGT+CGG + Chr1:90397562-90397581 None:intergenic 40.0%
GCATCTCTATATTGGGAAGA+TGG - Chr1:90399682-90399701 MsG0180005329.01.T01:intron 40.0%
GCTGTTTGCTTTGGTATAGT+AGG - Chr1:90398562-90398581 MsG0180005329.01.T01:intron 40.0%
GTTTAGCCCAACAAGTAACA+AGG + Chr1:90399864-90399883 None:intergenic 40.0%
TAGGAACTGGTATGTTAGTG+GGG + Chr1:90398413-90398432 None:intergenic 40.0%
TAGTCAATGAACATGCAGTC+AGG + Chr1:90399413-90399432 None:intergenic 40.0%
TCAGATTGAGGAGTGAATCA+AGG - Chr1:90400074-90400093 MsG0180005329.01.T01:CDS 40.0%
TGATATAAGTGGTGGCTTTC+GGG - Chr1:90399438-90399457 MsG0180005329.01.T01:intron 40.0%
TGCTACAACAACATAGGAAC+TGG + Chr1:90398426-90398445 None:intergenic 40.0%
TGTATAGTAATAAGCAGGGC+CGG - Chr1:90398304-90398323 MsG0180005329.01.T01:CDS 40.0%
TGTTAAAACTGTTCTTCCCC+AGG - Chr1:90399900-90399919 MsG0180005329.01.T01:CDS 40.0%
TTGCTTTGGTATAGTAGGCT+GGG - Chr1:90398567-90398586 MsG0180005329.01.T01:intron 40.0%
TTTGCTTTGGTATAGTAGGC+TGG - Chr1:90398566-90398585 MsG0180005329.01.T01:intron 40.0%
! ACAGAATTTGCTCGTTTGGT+GGG - Chr1:90399937-90399956 MsG0180005329.01.T01:CDS 40.0%
! GATCAGTGCTTCTGACATTT+TGG + Chr1:90397614-90397633 None:intergenic 40.0%
! GGTTACAGAATTTGCTCGTT+TGG - Chr1:90399933-90399952 MsG0180005329.01.T01:CDS 40.0%
! GTGAGGCTATCACTGTTTTA+GGG + Chr1:90399618-90399637 None:intergenic 40.0%
! TACAGAATTTGCTCGTTTGG+TGG - Chr1:90399936-90399955 MsG0180005329.01.T01:CDS 40.0%
! TTTGGTTTCTTTCCTGTTGC+TGG - Chr1:90398513-90398532 MsG0180005329.01.T01:intron 40.0%
! TTTTGAGCCTGTTCAGATTG+AGG - Chr1:90400062-90400081 MsG0180005329.01.T01:CDS 40.0%
!! ATGCTTTGCTGTTGCTGTTT+CGG - Chr1:90398445-90398464 MsG0180005329.01.T01:intron 40.0%
!! CCGATTTGAGTTGACCAAAA+CGG + Chr1:90398059-90398078 None:intergenic 40.0%
!! CCGTTTTGGTCAACTCAAAT+CGG - Chr1:90398056-90398075 MsG0180005329.01.T01:CDS 40.0%
!! GGATAATGGGGTTCAGTTTA+TGG - Chr1:90397969-90397988 MsG0180005329.01.T01:CDS 40.0%
!! TCAGAAGCACTGATCCAAAT+CGG - Chr1:90397619-90397638 MsG0180005329.01.T01:three_prime_UTR 40.0%
!!! CCTAGCTCTGCTGTTATTTT+TGG - Chr1:90398100-90398119 MsG0180005329.01.T01:CDS 40.0%
!!! CTGTTGCTGTTTCGGTATTT+TGG - Chr1:90398453-90398472 MsG0180005329.01.T01:intron 40.0%
!!! TGTTTTAGGGAGTTCGTAGT+TGG + Chr1:90399605-90399624 None:intergenic 40.0%
AAGCAATGTGGTCAGGATGT+AGG - Chr1:90398783-90398802 MsG0180005329.01.T01:intron 45.0%
AATCAAATTTGCCGCTCCGT+TGG - Chr1:90399178-90399197 MsG0180005329.01.T01:intron 45.0%
ACAATTGGCCACTTCTTCTC+AGG + Chr1:90399988-90400007 None:intergenic 45.0%
ACCGATCCTACTTACGAACA+TGG + Chr1:90398844-90398863 None:intergenic 45.0%
ACTCTAAGCCTGAGAAGAAG+TGG - Chr1:90399977-90399996 MsG0180005329.01.T01:CDS 45.0%
AGATAGAGAGTGAGTGAGTG+TGG + Chr1:90397577-90397596 None:intergenic 45.0%
AGCCTCCAAATTCGTTGGAA+CGG - Chr1:90398738-90398757 MsG0180005329.01.T01:intron 45.0%
AGGAACTGGTATGTTAGTGG+GGG + Chr1:90398412-90398431 None:intergenic 45.0%
ATATACACAGCGTCCACATC+AGG + Chr1:90397823-90397842 None:intergenic 45.0%
ATCAAATTTGCCGCTCCGTT+GGG - Chr1:90399179-90399198 MsG0180005329.01.T01:intron 45.0%
ATGTGGTCAGGATGTAGGAT+TGG - Chr1:90398788-90398807 MsG0180005329.01.T01:intron 45.0%
CAGAAAAAAAGGCCAGCAAC+AGG + Chr1:90398528-90398547 None:intergenic 45.0%
CGTACACCTTCGTTGTTAGA+GGG + Chr1:90397774-90397793 None:intergenic 45.0%
CTCTGCTCAATTGGCATCTT+TGG - Chr1:90400138-90400157 MsG0180005329.01.T01:CDS 45.0%
CTGATCCAAATCGGAGTAGT+AGG - Chr1:90397628-90397647 MsG0180005329.01.T01:three_prime_UTR 45.0%
TAAAACTGTTCTTCCCCAGG+AGG - Chr1:90399903-90399922 MsG0180005329.01.T01:CDS 45.0%
TAGGGGCTTGTGAGGATAAT+GGG - Chr1:90397956-90397975 MsG0180005329.01.T01:CDS 45.0%
TATACACAGCGTCCACATCA+GGG + Chr1:90397822-90397841 None:intergenic 45.0%
TGAAATTGTAGGGGCTTGTG+AGG - Chr1:90397948-90397967 MsG0180005329.01.T01:CDS 45.0%
TGTAACCAAAAGAGCCTCCT+GGG + Chr1:90399920-90399939 None:intergenic 45.0%
TGTTCGTAAGTAGGATCGGT+AGG - Chr1:90398844-90398863 MsG0180005329.01.T01:intron 45.0%
TTCCAACGAATTTGGAGGCT+CGG + Chr1:90398738-90398757 None:intergenic 45.0%
TTGTAGTCAGGATCGAGTGT+TGG + Chr1:90399048-90399067 None:intergenic 45.0%
! CCTCTAACAACGAAGGTGTA+CGG - Chr1:90397772-90397791 MsG0180005329.01.T01:CDS 45.0%
! CGTGAGGCTATCACTGTTTT+AGG + Chr1:90399619-90399638 None:intergenic 45.0%
! CTCTAACAACGAAGGTGTAC+GGG - Chr1:90397773-90397792 MsG0180005329.01.T01:CDS 45.0%
! TATTTCAAGTGCCAAGCACC+AGG + Chr1:90398636-90398655 None:intergenic 45.0%
! TTCAATGATCCGCACCGTTT+TGG - Chr1:90398042-90398061 MsG0180005329.01.T01:CDS 45.0%
! TTTGCCATTTAGTGCACTCC+TGG - Chr1:90398615-90398634 MsG0180005329.01.T01:intron 45.0%
!! AGATGCAAGCTTGTTGGTAG+TGG + Chr1:90397847-90397866 None:intergenic 45.0%
!! AGGATCGAGTGTTGGTTTGT+AGG + Chr1:90399040-90399059 None:intergenic 45.0%
!! GCTGTTTCGGTATTTTGGGA+GGG - Chr1:90398458-90398477 MsG0180005329.01.T01:intron 45.0%
!! TCATTTTAGTCCCAACGGAG+CGG + Chr1:90399192-90399211 None:intergenic 45.0%
!! TTGAGTTGACCAAAACGGTG+CGG + Chr1:90398054-90398073 None:intergenic 45.0%
!! TTTTTGGAGGCCCATACCTT+GGG + Chr1:90398369-90398388 None:intergenic 45.0%
!!! TGCTGTTTCGGTATTTTGGG+AGG - Chr1:90398457-90398476 MsG0180005329.01.T01:intron 45.0%
!!! TGTGCCATTTTAGCAGTCCT+AGG + Chr1:90398018-90398037 None:intergenic 45.0%
!!! TTTTTTGGAGGCCCATACCT+TGG + Chr1:90398370-90398389 None:intergenic 45.0%
AACTGGTATGTTAGTGGGGG+GGG + Chr1:90398409-90398428 None:intergenic 50.0%
AAGCACGGTTGAGATGTCGT+GGG - Chr1:90399005-90399024 MsG0180005329.01.T01:intron 50.0%
AAGCCAGATCTTGACGCACA+TGG - Chr1:90399529-90399548 MsG0180005329.01.T01:intron 50.0%
ACCAAAGCAAACAGCGGCAA+AGG + Chr1:90398557-90398576 None:intergenic 50.0%
ACCGTGCTTCGACTTTATGC+AGG + Chr1:90398994-90399013 None:intergenic 50.0%
AGGGGCTTGTGAGGATAATG+GGG - Chr1:90397957-90397976 MsG0180005329.01.T01:CDS 50.0%
CACAGCCTACTACTCCGATT+TGG + Chr1:90397636-90397655 None:intergenic 50.0%
CCCTGCATAAAGTCGAAGCA+CGG - Chr1:90398990-90399009 MsG0180005329.01.T01:intron 50.0%
CCGTACACCTTCGTTGTTAG+AGG + Chr1:90397775-90397794 None:intergenic 50.0%
CCGTGCTTCGACTTTATGCA+GGG + Chr1:90398993-90399012 None:intergenic 50.0%
CGTTGTTAGAGGGTAGCCAT+TGG + Chr1:90397764-90397783 None:intergenic 50.0%
CTGTAACCAAAAGAGCCTCC+TGG + Chr1:90399921-90399940 None:intergenic 50.0%
CTTATTCTGGGCCGCAAGAA+CGG + Chr1:90397878-90397897 None:intergenic 50.0%
GAACTGGTATGTTAGTGGGG+GGG + Chr1:90398410-90398429 None:intergenic 50.0%
GGAACTGGTATGTTAGTGGG+GGG + Chr1:90398411-90398430 None:intergenic 50.0%
GTAACCAAAAGAGCCTCCTG+GGG + Chr1:90399919-90399938 None:intergenic 50.0%
GTAGGGGCTTGTGAGGATAA+TGG - Chr1:90397955-90397974 MsG0180005329.01.T01:CDS 50.0%
GTTCGTAGTTGGCAGCTAAG+AGG + Chr1:90399594-90399613 None:intergenic 50.0%
TCAGCAATATCCAGCGCAAC+AGG + Chr1:90397922-90397941 None:intergenic 50.0%
TCCTTTGCCGCTGTTTGCTT+TGG - Chr1:90398553-90398572 MsG0180005329.01.T01:intron 50.0%
TCTACTGGAAGACCCTGATG+TGG - Chr1:90397807-90397826 MsG0180005329.01.T01:CDS 50.0%
TCTTAAGTGGGCCGTTCTTG+CGG - Chr1:90397864-90397883 MsG0180005329.01.T01:CDS 50.0%
TGGCTACCCTCTAACAACGA+AGG - Chr1:90397765-90397784 MsG0180005329.01.T01:CDS 50.0%
TGTTAGAGGGTAGCCATTGG+CGG + Chr1:90397761-90397780 None:intergenic 50.0%
TTTAGTGCACTCCTGGTGCT+TGG - Chr1:90398622-90398641 MsG0180005329.01.T01:intron 50.0%
! AGCACCAGGAGTGCACTAAA+TGG + Chr1:90398622-90398641 None:intergenic 50.0%
! GGCGTTTGGGGATAAGTTGA+TGG + Chr1:90397686-90397705 None:intergenic 50.0%
! GGTCAGGATGTAGGATTGGT+CGG - Chr1:90398792-90398811 MsG0180005329.01.T01:intron 50.0%
!! GTTAAGCACTGGTTCACGTG+AGG + Chr1:90399635-90399654 None:intergenic 50.0%
!! TCGGTGCCGGAAGTAAAATG+AGG + Chr1:90398719-90398738 None:intergenic 50.0%
!! TTTTGGAGGCCCATACCTTG+GGG + Chr1:90398368-90398387 None:intergenic 50.0%
CAAATTCGTTGGAACGGCCC+TGG - Chr1:90398744-90398763 MsG0180005329.01.T01:intron 55.0%
CACCGAGCCTCCAAATTCGT+TGG - Chr1:90398733-90398752 MsG0180005329.01.T01:intron 55.0%
CAGGGCCGTTCCAACGAATT+TGG + Chr1:90398746-90398765 None:intergenic 55.0%
CATGGCTCTCTAGGTGATGC+AGG - Chr1:90399547-90399566 MsG0180005329.01.T01:intron 55.0%
CGGGAACTACGAAGCTCTAC+TGG - Chr1:90397792-90397811 MsG0180005329.01.T01:CDS 55.0%
CTTGACGCACATGGCTCTCT+AGG - Chr1:90399538-90399557 MsG0180005329.01.T01:intron 55.0%
GAAGCACGGTTGAGATGTCG+TGG - Chr1:90399004-90399023 MsG0180005329.01.T01:intron 55.0%
GAGCCATGTGCGTCAAGATC+TGG + Chr1:90399535-90399554 None:intergenic 55.0%
GCTCTCTAGGTGATGCAGGT+TGG - Chr1:90399551-90399570 MsG0180005329.01.T01:intron 55.0%
GGCCGTTCCAACGAATTTGG+AGG + Chr1:90398743-90398762 None:intergenic 55.0%
TCTTGAGAAGCCTGTTGCGC+TGG - Chr1:90397909-90397928 MsG0180005329.01.T01:CDS 55.0%
! TCTTCCCCAGGAGGCTCTTT+TGG - Chr1:90399912-90399931 MsG0180005329.01.T01:CDS 55.0%
!! ATGTTAGTGGGGGGGGTACT+AGG + Chr1:90398402-90398421 None:intergenic 55.0%
!! CGGTGGCTTTGTCGTAGTTG+CGG + Chr1:90397729-90397748 None:intergenic 55.0%
AATAAGCAGGGCCGGACCCT+GGG - Chr1:90398312-90398331 MsG0180005329.01.T01:CDS 60.0%
ACCAGGCCTCCCCAAGGTAT+GGG - Chr1:90398356-90398375 MsG0180005329.01.T01:intron 60.0%
GCAAGCAGCGCTATAGCACC+AGG - Chr1:90398339-90398358 MsG0180005329.01.T01:intron 60.0%
TAATAAGCAGGGCCGGACCC+TGG - Chr1:90398311-90398330 MsG0180005329.01.T01:CDS 60.0%
TAGAGGGTAGCCATTGGCGG+CGG + Chr1:90397758-90397777 None:intergenic 60.0%
TATAGCACCAGGCCTCCCCA+AGG - Chr1:90398350-90398369 MsG0180005329.01.T01:intron 60.0%
TGGTGGCTTTCGGGCTGTAC+AGG - Chr1:90399447-90399466 MsG0180005329.01.T01:intron 60.0%
! GGCTTTGTCGTAGTTGCGGC+TGG + Chr1:90397725-90397744 None:intergenic 60.0%
!! CGAATTTGGAGGCTCGGTGC+CGG + Chr1:90398732-90398751 None:intergenic 60.0%
ATAAGCAGGGCCGGACCCTG+GGG - Chr1:90398313-90398332 MsG0180005329.01.T01:CDS 65.0%
CACCAGGCCTCCCCAAGGTA+TGG - Chr1:90398355-90398374 MsG0180005329.01.T01:intron 65.0%
GTCGTAGTTGCGGCTGGCGA+CGG + Chr1:90397719-90397738 None:intergenic 65.0%
TGGAGGCCCATACCTTGGGG+AGG + Chr1:90398365-90398384 None:intergenic 65.0%
! ACGGCGCAGATGGTGGCGTT+TGG + Chr1:90397700-90397719 None:intergenic 65.0%
! CGGCGCAGATGGTGGCGTTT+GGG + Chr1:90397699-90397718 None:intergenic 65.0%
! GGCGCAGATGGTGGCGTTTG+GGG + Chr1:90397698-90397717 None:intergenic 65.0%
ACCGCTTTCGCCGCCGCCAA+TGG - Chr1:90397745-90397764 MsG0180005329.01.T01:CDS 70.0%
AGCGCTGCTTGCACCCCCCA+GGG + Chr1:90398331-90398350 None:intergenic 70.0%
ATTGGCGGCGGCGAAAGCGG+TGG + Chr1:90397746-90397765 None:intergenic 70.0%
GCCATTGGCGGCGGCGAAAG+CGG + Chr1:90397749-90397768 None:intergenic 70.0%
GCCCATACCTTGGGGAGGCC+TGG + Chr1:90398360-90398379 None:intergenic 70.0%
TAAGCAGGGCCGGACCCTGG+GGG - Chr1:90398314-90398333 MsG0180005329.01.T01:CDS 70.0%
TAGCGCTGCTTGCACCCCCC+AGG + Chr1:90398332-90398351 None:intergenic 70.0%
TGCTTGCACCCCCCAGGGTC+CGG + Chr1:90398326-90398345 None:intergenic 70.0%
AAGCAGGGCCGGACCCTGGG+GGG - Chr1:90398315-90398334 MsG0180005329.01.T01:CDS 75.0%
GCTGGCGACGGCGCAGATGG+TGG + Chr1:90397707-90397726 None:intergenic 75.0%
! GCGGCTGGCGACGGCGCAGA+TGG + Chr1:90397710-90397729 None:intergenic 80.0%
Chromosome Type Strat End Strand Name
Chr1 gene 90397536 90400282 90397536 ID=MsG0180005329.01;Name=MsG0180005329.01
Chr1 mRNA 90397536 90400282 90397536 ID=MsG0180005329.01.T01;Parent=MsG0180005329.01;Name=MsG0180005329.01.T01;_AED=0.47;_eAED=0.47;_QI=80|1|1|1|1|1|2|193|360
Chr1 exon 90397536 90398349 90397536 ID=MsG0180005329.01.T01:exon:47988;Parent=MsG0180005329.01.T01
Chr1 exon 90399741 90400282 90399741 ID=MsG0180005329.01.T01:exon:47987;Parent=MsG0180005329.01.T01
Chr1 five_prime_UTR 90400203 90400282 90400203 ID=MsG0180005329.01.T01:five_prime_utr;Parent=MsG0180005329.01.T01
Chr1 CDS 90399741 90400202 90399741 ID=MsG0180005329.01.T01:cds;Parent=MsG0180005329.01.T01
Chr1 CDS 90397729 90398349 90397729 ID=MsG0180005329.01.T01:cds;Parent=MsG0180005329.01.T01
Chr1 three_prime_UTR 90397536 90397728 90397536 ID=MsG0180005329.01.T01:three_prime_utr;Parent=MsG0180005329.01.T01
Gene Sequence

>MsG0180005329.01.T01

ATGTCAGAAGCACTGATCCAAATCGGAGTAGTAGGCTGTGCCGACATAGCTCGTAAAGTTTCTCGCGCCATCAACTTATCCCCAAACGCCACCATCTGCGCCGTCGCCAGCCGCAACTACGACAAAGCCACCGCTTTCGCCGCCGCCAATGGCTACCCTCTAACAACGAAGGTGTACGGGAACTACGAAGCTCTACTGGAAGACCCTGATGTGGACGCTGTGTATATGCCACTACCAACAAGCTTGCATCTTAAGTGGGCCGTTCTTGCGGCCCAGAATAAGAAGCATTTGCTTCTTGAGAAGCCTGTTGCGCTGGATATTGCTGAGTTTGATGAAATTGTAGGGGCTTGTGAGGATAATGGGGTTCAGTTTATGGATAATACTATGTGGGTTCATAATCCTAGGACTGCTAAAATGGCACAGTTCTTCAATGATCCGCACCGTTTTGGTCAACTCAAATCGGTTCGCACCTGCTTTACATTTGCTGCAGATTCTGAATATCTAAAGAAAGACATTCGTGTGAAGCCAGATCTTGACGCACATGGCTCTCTAGGTGATGCAGGTTGGTATTGCATTCGTGCAATCCTCTTAGCTGCCAACTACGAACTCCCTAAAACAGTGATAGCCTCACGTGAACCAGTGCTTAACAAAGATGGAGTTATATTGGATTGTGGAGCATCTCTATATTGGGAAGATGGTAAAGTAGCAACATTCCATTGTTCTTTCTTGGCAAACTTGACAATGGATATAACAGCTATTGGAACAAAAGGAACTCTTCATGTTCATGATTTTATTATTCCTTATGAAGAGAATGAAGCATCTTTTCATGTTGGTACTGAAACTAGCTTTGATGACCTTGTTACTTGTTGGGCTAAACAGCCAATTAAGTGTACTGTTAAAACTGTTCTTCCCCAGGAGGCTCTTTTGGTTACAGAATTTGCTCGTTTGGTGGGTGAAATTAAATTTAGAAACTCTAAGCCTGAGAAGAAGTGGCCAATTGTTAGTAGGAAAACACAACTTGTGTTGAATGCTGTTAAGTCTTCAATTCATTGGGGTTTTGAGCCTGTTCAGATTGAGGAGTGA
Protein sequence

>MsG0180005329.01.T01

MSEALIQIGVVGCADIARKVSRAINLSPNATICAVASRNYDKATAFAAANGYPLTTKVYGNYEALLEDPDVDAVYMPLPTSLHLKWAVLAAQNKKHLLLEKPVALDIAEFDEIVGACEDNGVQFMDNTMWVHNPRTAKMAQFFNDPHRFGQLKSVRTCFTFAADSEYLKKDIRVKPDLDAHGSLGDAGWYCIRAILLAANYELPKTVIASREPVLNKDGVILDCGASLYWEDGKVATFHCSFLANLTMDITAIGTKGTLHVHDFIIPYEENEASFHVGTETSFDDLVTCWAKQPIKCTVKTVLPQEALLVTEFARLVGEIKFRNSKPEKKWPIVSRKTQLVLNAVKSSIHWGFEPVQIEE*