AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0380012367.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380012367.01.T01 MTR_3g023120 60.185 108 28 3 1 108 1 93 4.46e-32 111
MsG0380012367.01.T01 MTR_3g023120 62.136 103 24 3 1 103 1 88 5.32e-32 110
MsG0380012367.01.T01 MTR_5g029240 52.885 104 34 3 5 108 14 102 2.18e-24 92.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0380012367.01.T01 AT1G32210 49.231 130 51 3 1 130 1 115 2.42e-27 99.8
MsG0380012367.01.T01 AT1G32210 58.252 103 28 3 1 103 1 88 4.38e-27 98.6
MsG0380012367.01.T01 AT2G35520 46.154 130 56 2 1 130 1 116 7.88e-26 95.9
MsG0380012367.01.T01 AT2G35520 46.923 130 54 3 1 130 1 115 5.15e-25 93.6
MsG0380012367.01.T01 AT2G35520 46.923 130 54 3 1 130 1 115 5.15e-25 93.6

Find 33 sgRNAs with CRISPR-Local

Find 227 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTGGTGATCATGAACTTTCT+TGG 0.304837 3:-15542206 MsG0380012367.01.T01:CDS
CCTTGTTCTCTTTATTCACT+TGG 0.305994 3:+15542528 None:intergenic
AAGATCTTCTCCGCACTCTT+TGG 0.356756 3:-15544334 MsG0380012367.01.T01:CDS
CTGCAATGTGGTGCTCCATT+TGG 0.370798 3:-15542225 MsG0380012367.01.T01:CDS
CATGAACTTTCTTGGTTAAG+TGG 0.399313 3:-15542198 MsG0380012367.01.T01:CDS
GATAGATTCTTACAGATGAT+TGG 0.406226 3:-15542300 MsG0380012367.01.T01:CDS
ATGATTGGTATTTCATATAC+AGG 0.419960 3:-15542285 MsG0380012367.01.T01:CDS
TCTCTTTATTCACTTGGATC+CGG 0.452230 3:+15542534 None:intergenic
AAAATCTGCAAAAGCGCGCT+CGG 0.463481 3:+15542253 None:intergenic
TAAAGAGAACAAGGAATTCA+AGG 0.465642 3:-15542519 MsG0380012367.01.T01:intron
AGGAACAACCTTGAGATTTG+TGG 0.468795 3:+15544292 None:intergenic
GGAACAACCTTGAGATTTGT+GGG 0.478791 3:+15544293 None:intergenic
CACTTGGATCCGGAGACAAC+TGG 0.482847 3:+15542544 None:intergenic
AAATCTGCAAAAGCGCGCTC+GGG 0.496102 3:+15542254 None:intergenic
ATGAACTTTCTTGGTTAAGT+GGG 0.503819 3:-15542197 MsG0380012367.01.T01:CDS
TGTCTTCACTGCCCTCATCC+AGG 0.517420 3:-15544179 MsG0380012367.01.T01:CDS
GAAAGTTCATGATCACCAAA+TGG 0.518501 3:+15542210 None:intergenic
CTTGGTTAAGTGGGTTTGTG+TGG 0.522677 3:-15542188 MsG0380012367.01.T01:CDS
GGAAAGGAAGTACCTGGATG+AGG 0.534843 3:+15544167 None:intergenic
ACAATAGTTCTAATCTGCCT+AGG 0.562587 3:-15542628 MsG0380012367.01.T01:CDS
CCAAGTGAATAAAGAGAACA+AGG 0.563011 3:-15542528 MsG0380012367.01.T01:CDS
TTAGAAATTGAAGCAAGGAA+AGG 0.564891 3:+15544151 None:intergenic
GAAATGATGAAACAATGTGT+TGG 0.569466 3:-15542338 MsG0380012367.01.T01:CDS
AAGCAAGGAAAGGAAGTACC+TGG 0.573337 3:+15544161 None:intergenic
GATGGTGAAGTCTACTGCTA+AGG 0.589667 3:-15544363 MsG0380012367.01.T01:CDS
GAAAGGAAGTACCTGGATGA+GGG 0.591104 3:+15544168 None:intergenic
AAACTTATGCATATGAACCT+AGG 0.600668 3:+15542611 None:intergenic
GATCTTTAGAAATTGAAGCA+AGG 0.608862 3:+15544146 None:intergenic
CAGACAACTACGACAGACGC+TGG 0.617073 3:+15545635 None:intergenic
AACAACCTTGAGATTTGTGG+GGG 0.618905 3:+15544295 None:intergenic
GAACAACCTTGAGATTTGTG+GGG 0.633022 3:+15544294 None:intergenic
TGTCTGAATTTCGAATCCAA+AGG 0.647407 3:-15545618 MsG0380012367.01.T01:intron
AATAAGCAGACCAAAGAGTG+CGG 0.691016 3:+15544324 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CAGAAAAAATAGAATAAAAT+TGG + Chr3:15542349-15542368 None:intergenic 15.0%
!!! GTTTTTAAAAAATTAGACTT+GGG + Chr3:15544883-15544902 None:intergenic 15.0%
!!! TACAAGTGAAATTAATATTT+TGG - Chr3:15544909-15544928 MsG0380012367.01.T01:intron 15.0%
!!! TGTTTTTAAAAAATTAGACT+TGG + Chr3:15544884-15544903 None:intergenic 15.0%
!! AATGAATGAAACAATTGAAT+TGG + Chr3:15542272-15542291 None:intergenic 20.0%
!! ACTAATCTGATTATGATATT+CGG + Chr3:15543797-15543816 None:intergenic 20.0%
!! CTCATATATTGTTCTTATAT+TGG - Chr3:15543231-15543250 MsG0380012367.01.T01:intron 20.0%
!! TCAAAATAAAAAAGCTGATT+TGG + Chr3:15543820-15543839 None:intergenic 20.0%
!! TCATTAAACTAGAATCTAAA+AGG + Chr3:15544857-15544876 None:intergenic 20.0%
!! TTGTATTAATGTCATTATTC+TGG - Chr3:15545412-15545431 MsG0380012367.01.T01:intron 20.0%
!! TTTCAAATTATTAGTTGTGT+CGG + Chr3:15542757-15542776 None:intergenic 20.0%
!!! GAAATTAATATTTTGGTGAT+GGG - Chr3:15544916-15544935 MsG0380012367.01.T01:intron 20.0%
!!! TGAAATTAATATTTTGGTGA+TGG - Chr3:15544915-15544934 MsG0380012367.01.T01:intron 20.0%
! AACATCATAATACACAAGAA+AGG + Chr3:15545357-15545376 None:intergenic 25.0%
! AACTCCTAATTCCTAATTAA+TGG + Chr3:15544586-15544605 None:intergenic 25.0%
! AAGATACATGAAATATTCAG+AGG + Chr3:15545463-15545482 None:intergenic 25.0%
! AGAGTAAGATTAAAATTGGA+GGG + Chr3:15544819-15544838 None:intergenic 25.0%
! ATGATTGGTATTTCATATAC+AGG - Chr3:15545558-15545577 MsG0380012367.01.T01:intron 25.0%
! CTTTATGTGTGTTATGTAAT+TGG + Chr3:15544063-15544082 None:intergenic 25.0%
! CTTTGTGGGAAAATATTTAT+AGG - Chr3:15543083-15543102 MsG0380012367.01.T01:intron 25.0%
! TAATGACTGAACAAATAGAA+AGG - Chr3:15545483-15545502 MsG0380012367.01.T01:intron 25.0%
! TAGAGTAAGATTAAAATTGG+AGG + Chr3:15544820-15544839 None:intergenic 25.0%
! TATATGCAATAACATGTGTT+TGG + Chr3:15543959-15543978 None:intergenic 25.0%
! TCTAAAAGGATAGTATTATC+TGG + Chr3:15544843-15544862 None:intergenic 25.0%
! TCTCACATAAAAAAGACATA+AGG + Chr3:15542902-15542921 None:intergenic 25.0%
! TGCAGGAAATACAAATATAT+AGG - Chr3:15544361-15544380 MsG0380012367.01.T01:CDS 25.0%
! TGGTAGAGTAAGATTAAAAT+TGG + Chr3:15544823-15544842 None:intergenic 25.0%
!! AAATCAATCTCTGATTTTTG+TGG - Chr3:15543766-15543785 MsG0380012367.01.T01:intron 25.0%
!! ATCTAGATAACAGTTTCTTT+TGG + Chr3:15544087-15544106 None:intergenic 25.0%
!! GATTGATTTACTAATGTTAC+AGG + Chr3:15543755-15543774 None:intergenic 25.0%
!! TCTAGATAACAGTTTCTTTT+GGG + Chr3:15544086-15544105 None:intergenic 25.0%
!! TTTGATTACTGTTTGATGAT+TGG - Chr3:15543835-15543854 MsG0380012367.01.T01:intron 25.0%
!! TTTTCATTTTCTTAATGACC+AGG - Chr3:15545023-15545042 MsG0380012367.01.T01:intron 25.0%
!!! ATATTTTTGTAGAAATGCAG+AGG + Chr3:15543583-15543602 None:intergenic 25.0%
AAAAACAAATGCAGGTAGAA+AGG + Chr3:15542557-15542576 None:intergenic 30.0%
AAAAAGTTTCCTATGAAGCA+CGG + Chr3:15542875-15542894 None:intergenic 30.0%
AAAACAAAGTTAACTTCCAC+GGG - Chr3:15544307-15544326 MsG0380012367.01.T01:CDS 30.0%
AAAACAAATGCAGGTAGAAA+GGG + Chr3:15542556-15542575 None:intergenic 30.0%
AAACTTATGCATATGAACCT+AGG + Chr3:15545235-15545254 None:intergenic 30.0%
AAGAAAGATCACAAATGTTG+TGG + Chr3:15544759-15544778 None:intergenic 30.0%
AATCTGATTATGATATTCGG+AGG + Chr3:15543794-15543813 None:intergenic 30.0%
AATTCACCAAATCATCTTGA+AGG - Chr3:15544187-15544206 MsG0380012367.01.T01:CDS 30.0%
ACATAACCATTGAACCTATA+AGG + Chr3:15542465-15542484 None:intergenic 30.0%
ATCTTGAAGGAAGTAGATTA+GGG - Chr3:15544200-15544219 MsG0380012367.01.T01:CDS 30.0%
ATGAACTTTCTTGGTTAAGT+GGG - Chr3:15545646-15545665 MsG0380012367.01.T01:five_prime_UTR 30.0%
ATGTCTGGAAGGATATTATT+TGG + Chr3:15544784-15544803 None:intergenic 30.0%
CAAAACAAAGTTAACTTCCA+CGG - Chr3:15544306-15544325 MsG0380012367.01.T01:CDS 30.0%
GAAATGATGAAACAATGTGT+TGG - Chr3:15545505-15545524 MsG0380012367.01.T01:intron 30.0%
GAAGAAAAAGTTGAAAACGA+TGG - Chr3:15543462-15543481 MsG0380012367.01.T01:intron 30.0%
GATAGATTCTTACAGATGAT+TGG - Chr3:15545543-15545562 MsG0380012367.01.T01:intron 30.0%
GTGAAGAGTTCTATGATATT+TGG + Chr3:15544715-15544734 None:intergenic 30.0%
TAAAGAGAACAAGGAATTCA+AGG - Chr3:15545324-15545343 MsG0380012367.01.T01:intron 30.0%
TATTGAAACTAACTCTTCAG+AGG - Chr3:15542661-15542680 MsG0380012367.01.T01:intron 30.0%
TCTTCTATTTCAGCATCTAT+AGG - Chr3:15543396-15543415 MsG0380012367.01.T01:intron 30.0%
TGTAAACTGAGATTCTTTGT+GGG - Chr3:15543069-15543088 MsG0380012367.01.T01:intron 30.0%
TTAGAAATTGAAGCAAGGAA+AGG + Chr3:15543695-15543714 None:intergenic 30.0%
TTCGAAGAATACAAGAATCT+GGG - Chr3:15542933-15542952 MsG0380012367.01.T01:intron 30.0%
TTGTAAACTGAGATTCTTTG+TGG - Chr3:15543068-15543087 MsG0380012367.01.T01:intron 30.0%
TTTAGTGTAATTCCAAGTGT+CGG - Chr3:15542787-15542806 MsG0380012367.01.T01:intron 30.0%
TTTCGAAGAATACAAGAATC+TGG - Chr3:15542932-15542951 MsG0380012367.01.T01:intron 30.0%
! GATCTTTAGAAATTGAAGCA+AGG + Chr3:15543700-15543719 None:intergenic 30.0%
! TGAATTTTTGTGACTTGAAG+TGG - Chr3:15543359-15543378 MsG0380012367.01.T01:intron 30.0%
!! ATCATTGATTTGAGATCAGA+CGG - Chr3:15544620-15544639 MsG0380012367.01.T01:intron 30.0%
!!! ACCTGCATTTGTTTTTTTGT+AGG - Chr3:15542561-15542580 MsG0380012367.01.T01:CDS 30.0%
!!! AGTTAACTTTGTTTTGCTGT+CGG + Chr3:15544302-15544321 None:intergenic 30.0%
!!! ATATTTTGGTGATGGGTTAA+TGG - Chr3:15544923-15544942 MsG0380012367.01.T01:intron 30.0%
!!! TGTCTTTTTTATGTGAGACT+TGG - Chr3:15542904-15542923 MsG0380012367.01.T01:intron 30.0%
!!! TTTCTCTTTTTTCACTGTGT+AGG - Chr3:15543437-15543456 MsG0380012367.01.T01:intron 30.0%
AAACAAATGCAGGTAGAAAG+GGG + Chr3:15542555-15542574 None:intergenic 35.0%
ACAAATATATAGGCTGCACA+TGG - Chr3:15544371-15544390 MsG0380012367.01.T01:exon 35.0%
ACTGGTCAAAAAATAGAGGA+TGG + Chr3:15545284-15545303 None:intergenic 35.0%
ATATACCTGAATACGCTATG+GGG - Chr3:15544414-15544433 MsG0380012367.01.T01:intron 35.0%
ATCTGTGCTTCATAGGTTAA+GGG + Chr3:15542692-15542711 None:intergenic 35.0%
ATTCCTCAATAATGTCTGGA+AGG + Chr3:15544795-15544814 None:intergenic 35.0%
ATTGAATTGGATCTGAGTGA+AGG + Chr3:15542259-15542278 None:intergenic 35.0%
CAGATTTGTGAAGTAACTTC+TGG + Chr3:15544009-15544028 None:intergenic 35.0%
CATCTTGAAGGAAGTAGATT+AGG - Chr3:15544199-15544218 MsG0380012367.01.T01:CDS 35.0%
CATGAACTTTCTTGGTTAAG+TGG - Chr3:15545645-15545664 MsG0380012367.01.T01:five_prime_UTR 35.0%
CATTTGCCTTATAGGTTCAA+TGG - Chr3:15542456-15542475 MsG0380012367.01.T01:intron 35.0%
CCAAGTGAATAAAGAGAACA+AGG - Chr3:15545315-15545334 MsG0380012367.01.T01:intron 35.0%
CCAGCCATTAATTAGGAATT+AGG - Chr3:15544579-15544598 MsG0380012367.01.T01:intron 35.0%
CCTAATTCCTAATTAATGGC+TGG + Chr3:15544582-15544601 None:intergenic 35.0%
CCTTGTTCTCTTTATTCACT+TGG + Chr3:15545318-15545337 None:intergenic 35.0%
CTAAATGAAAAGCCAAAGCA+GGG + Chr3:15543618-15543637 None:intergenic 35.0%
CTACTTCCTTCAAGATGATT+TGG + Chr3:15544196-15544215 None:intergenic 35.0%
CTTACATAAAATATCCCAGC+TGG + Chr3:15544455-15544474 None:intergenic 35.0%
CTTAGACAATGTTAGCATTG+GGG - Chr3:15543895-15543914 MsG0380012367.01.T01:intron 35.0%
GAAAGTTCATGATCACCAAA+TGG + Chr3:15545636-15545655 None:intergenic 35.0%
GACAACTGGTCAAAAAATAG+AGG + Chr3:15545288-15545307 None:intergenic 35.0%
GATATACCTGAATACGCTAT+GGG - Chr3:15544413-15544432 MsG0380012367.01.T01:intron 35.0%
GCCTACAAAAAAACAAATGC+AGG + Chr3:15542565-15542584 None:intergenic 35.0%
GCTTAGACAATGTTAGCATT+GGG - Chr3:15543894-15543913 MsG0380012367.01.T01:intron 35.0%
GTAACAGGAAATGAATGGAA+TGG + Chr3:15544556-15544575 None:intergenic 35.0%
GTGAATCGAATAGGTTACAT+AGG + Chr3:15544270-15544289 None:intergenic 35.0%
GTTATCTTAATCCATCCAGA+GGG - Chr3:15544511-15544530 MsG0380012367.01.T01:intron 35.0%
TAATGACCAGGTAGTTTACA+TGG - Chr3:15545035-15545054 MsG0380012367.01.T01:intron 35.0%
TATCCTTCCAGACATTATTG+AGG - Chr3:15544789-15544808 MsG0380012367.01.T01:intron 35.0%
TATCTGTGCTTCATAGGTTA+AGG + Chr3:15542693-15542712 None:intergenic 35.0%
TCTAAATGAAAAGCCAAAGC+AGG + Chr3:15543619-15543638 None:intergenic 35.0%
TCTCTTTATTCACTTGGATC+CGG + Chr3:15545312-15545331 None:intergenic 35.0%
TGCATCTTCATTTGCCTTAT+AGG - Chr3:15542448-15542467 MsG0380012367.01.T01:intron 35.0%
TGCTTAGACAATGTTAGCAT+TGG - Chr3:15543893-15543912 MsG0380012367.01.T01:intron 35.0%
TGTCTGAATTTCGAATCCAA+AGG - Chr3:15542225-15542244 MsG0380012367.01.T01:CDS 35.0%
TGTGACACGGTAAATAATCT+GGG + Chr3:15544646-15544665 None:intergenic 35.0%
TGTTATCTTAATCCATCCAG+AGG - Chr3:15544510-15544529 MsG0380012367.01.T01:intron 35.0%
TTGGCATTAAATGTGTGACA+CGG + Chr3:15544659-15544678 None:intergenic 35.0%
TTTGACCAATGATTCGAGAT+TGG - Chr3:15544150-15544169 MsG0380012367.01.T01:CDS 35.0%
! AGATTTTGTTCTCTGCAATG+TGG - Chr3:15545606-15545625 MsG0380012367.01.T01:intron 35.0%
! GCGATATAAACTTGATGACA+TGG + Chr3:15542599-15542618 None:intergenic 35.0%
! GTTTCTTGGATTAGGATTCT+TGG + Chr3:15544678-15544697 None:intergenic 35.0%
! TTACTGTTTGATGATTGGAG+TGG - Chr3:15543840-15543859 MsG0380012367.01.T01:intron 35.0%
! TTGGTGATCATGAACTTTCT+TGG - Chr3:15545637-15545656 MsG0380012367.01.T01:five_prime_UTR 35.0%
!! ACAATAGTTCTAATCTGCCT+AGG - Chr3:15545215-15545234 MsG0380012367.01.T01:intron 35.0%
!! TATTTTTGCTAACCCTGCTT+TGG - Chr3:15543603-15543622 MsG0380012367.01.T01:intron 35.0%
!! TCTGGATGGATTAAGATAAC+AGG + Chr3:15544511-15544530 None:intergenic 35.0%
!! TGTTGACTGCACATATCTTT+TGG - Chr3:15543166-15543185 MsG0380012367.01.T01:intron 35.0%
!!! ATTTTTTGACCAGTTGTCTC+CGG - Chr3:15545290-15545309 MsG0380012367.01.T01:intron 35.0%
AACAAATGCAGGTAGAAAGG+GGG + Chr3:15542554-15542573 None:intergenic 40.0%
AACAACCTTGAGATTTGTGG+GGG + Chr3:15543551-15543570 None:intergenic 40.0%
AATAAGCAGACCAAAGAGTG+CGG + Chr3:15543522-15543541 None:intergenic 40.0%
AGAAATTACGCCTGAAAGGA+AGG + Chr3:15545088-15545107 None:intergenic 40.0%
AGGAACAACCTTGAGATTTG+TGG + Chr3:15543554-15543573 None:intergenic 40.0%
ATAAAATATCCCAGCTGGCA+AGG + Chr3:15544450-15544469 None:intergenic 40.0%
ATAGTCCACAATCGCAATCT+CGG + Chr3:15543026-15543045 None:intergenic 40.0%
ATATCCGCAATCGCATATTC+TGG + Chr3:15544398-15544417 None:intergenic 40.0%
ATCGAATAGGTTACATAGGC+AGG + Chr3:15544266-15544285 None:intergenic 40.0%
ATGAAGCACAGATACAGACA+CGG - Chr3:15542699-15542718 MsG0380012367.01.T01:intron 40.0%
ATTCCTAATTAATGGCTGGC+AGG + Chr3:15544578-15544597 None:intergenic 40.0%
CAGGCGTAATTTCTTGCATA+GGG - Chr3:15545094-15545113 MsG0380012367.01.T01:intron 40.0%
CCATTTAACTCCTTCCTTTC+AGG - Chr3:15545075-15545094 MsG0380012367.01.T01:intron 40.0%
CCTCTTTCTTCCTTTGCTTT+GGG - Chr3:15544479-15544498 MsG0380012367.01.T01:intron 40.0%
CCTGAAAGGAAGGAGTTAAA+TGG + Chr3:15545078-15545097 None:intergenic 40.0%
GAACAACCTTGAGATTTGTG+GGG + Chr3:15543552-15543571 None:intergenic 40.0%
GGAAATGAATGGAATGGCAT+TGG + Chr3:15544550-15544569 None:intergenic 40.0%
GGAACAACCTTGAGATTTGT+GGG + Chr3:15543553-15543572 None:intergenic 40.0%
GGATATACCTGAATACGCTA+TGG - Chr3:15544412-15544431 MsG0380012367.01.T01:intron 40.0%
GGGAATTCCTCAATAATGTC+TGG + Chr3:15544799-15544818 None:intergenic 40.0%
GTGTGACACGGTAAATAATC+TGG + Chr3:15544647-15544666 None:intergenic 40.0%
GTGTGCATAAATTGCATAGC+TGG + Chr3:15545171-15545190 None:intergenic 40.0%
GTTATGTAATTGGATGACCG+TGG + Chr3:15544053-15544072 None:intergenic 40.0%
TAAAATATCCCAGCTGGCAA+GGG + Chr3:15544449-15544468 None:intergenic 40.0%
TAGAAATGCAGAGGAAAGAG+AGG + Chr3:15543574-15543593 None:intergenic 40.0%
TATCCGCAATCGCATATTCT+GGG + Chr3:15544397-15544416 None:intergenic 40.0%
TCAGGCGTAATTTCTTGCAT+AGG - Chr3:15545093-15545112 MsG0380012367.01.T01:intron 40.0%
TGACATTGTGCTGAATTTCC+CGG - Chr3:15543982-15544001 MsG0380012367.01.T01:intron 40.0%
TGCAAGAAATTACGCCTGAA+AGG + Chr3:15545092-15545111 None:intergenic 40.0%
TGCAGAATGCACTAGAAACA+TGG - Chr3:15544027-15544046 MsG0380012367.01.T01:intron 40.0%
TGCGTGCAATTTCTAATGCA+AGG + Chr3:15544111-15544130 None:intergenic 40.0%
TGTCTGTATCTGTGCTTCAT+AGG + Chr3:15542699-15542718 None:intergenic 40.0%
TTACCTGCCAGCCATTAATT+AGG - Chr3:15544572-15544591 MsG0380012367.01.T01:intron 40.0%
TTGTATTTCCTGCAATGTCC+AGG + Chr3:15544355-15544374 None:intergenic 40.0%
TTGTGAAGTAACTTCTGGAC+CGG + Chr3:15544004-15544023 None:intergenic 40.0%
! GCGAGTTCTCATACATTTTC+TGG + Chr3:15544239-15544258 None:intergenic 40.0%
!! AATGGAATGGCATTGGCTTA+TGG + Chr3:15544543-15544562 None:intergenic 40.0%
!! ATGGAATGGCATTGGCTTAT+GGG + Chr3:15544542-15544561 None:intergenic 40.0%
!! GTAGTTTACATGGCTTTGGT+GGG - Chr3:15545045-15545064 MsG0380012367.01.T01:intron 40.0%
AAGAGCAACACACGTTGAGT+AGG + Chr3:15542417-15542436 None:intergenic 45.0%
AAGATCTTCTCCGCACTCTT+TGG - Chr3:15543509-15543528 MsG0380012367.01.T01:intron 45.0%
ATAGGTTACATAGGCAGGAC+AGG + Chr3:15544261-15544280 None:intergenic 45.0%
ATGATATTTGGACTCCAGCG+AGG + Chr3:15544703-15544722 None:intergenic 45.0%
ATGCACTAGAAACATGGCCA+CGG - Chr3:15544033-15544052 MsG0380012367.01.T01:intron 45.0%
ATTAATGGCTGGCAGGTAAC+AGG + Chr3:15544571-15544590 None:intergenic 45.0%
CACAAATGTTGTGGTGATCG+AGG + Chr3:15544750-15544769 None:intergenic 45.0%
CCAAAGCCATGTAAACTACC+TGG + Chr3:15545044-15545063 None:intergenic 45.0%
CCAGGTAGTTTACATGGCTT+TGG - Chr3:15545041-15545060 MsG0380012367.01.T01:intron 45.0%
CCCAAAGCAAAGGAAGAAAG+AGG + Chr3:15544482-15544501 None:intergenic 45.0%
GATCTGCCATCGAAAGGAAA+TGG - Chr3:15544329-15544348 MsG0380012367.01.T01:CDS 45.0%
GATGGTGAAGTCTACTGCTA+AGG - Chr3:15543480-15543499 MsG0380012367.01.T01:intron 45.0%
GCCTCTTTCTTCCTTTGCTT+TGG - Chr3:15544478-15544497 MsG0380012367.01.T01:intron 45.0%
GCTCACCAATCTCGAATCAT+TGG + Chr3:15544158-15544177 None:intergenic 45.0%
GGCAGGTAACAGGAAATGAA+TGG + Chr3:15544561-15544580 None:intergenic 45.0%
GGGTGGAAGAAACTCTTATC+TGG - Chr3:15543915-15543934 MsG0380012367.01.T01:intron 45.0%
TGAAGGAAGTAGATTAGGGC+AGG - Chr3:15544204-15544223 MsG0380012367.01.T01:CDS 45.0%
TGTGAAGTAACTTCTGGACC+GGG + Chr3:15544003-15544022 None:intergenic 45.0%
! AAAATCTGCAAAAGCGCGCT+CGG + Chr3:15545593-15545612 None:intergenic 45.0%
! AGACAATGTTAGCATTGGGG+TGG - Chr3:15543898-15543917 MsG0380012367.01.T01:intron 45.0%
! CTAATTTTGTGAGCACGTCG+TGG - Chr3:15542975-15542994 MsG0380012367.01.T01:intron 45.0%
! CTTGGTTAAGTGGGTTTGTG+TGG - Chr3:15545655-15545674 MsG0380012367.01.T01:five_prime_UTR 45.0%
!! AAGCAAGGAAAGGAAGTACC+TGG + Chr3:15543685-15543704 None:intergenic 45.0%
!! GAAAGGAAGTACCTGGATGA+GGG + Chr3:15543678-15543697 None:intergenic 45.0%
!! GGTAGTTTACATGGCTTTGG+TGG - Chr3:15545044-15545063 MsG0380012367.01.T01:intron 45.0%
!! TGGAATGGCATTGGCTTATG+GGG + Chr3:15544541-15544560 None:intergenic 45.0%
!!! TTTGTTTTGCTGTCGGACCA+GGG + Chr3:15544295-15544314 None:intergenic 45.0%
AATCCAAGAAACTGCCTCGC+TGG - Chr3:15544686-15544705 MsG0380012367.01.T01:intron 50.0%
ACAGATACAGACACGGACAC+GGG - Chr3:15542706-15542725 MsG0380012367.01.T01:intron 50.0%
ACCAGGGGAGTGAATCGAAT+AGG + Chr3:15544279-15544298 None:intergenic 50.0%
ACCTATTCGATTCACTCCCC+TGG - Chr3:15544275-15544294 MsG0380012367.01.T01:intron 50.0%
AGTGAAGGCGAGGTTACCTT+TGG + Chr3:15542244-15542263 None:intergenic 50.0%
ATCAGCCCCATAGCGTATTC+AGG + Chr3:15544422-15544441 None:intergenic 50.0%
ATTGGATCTGAGTGAAGGCG+AGG + Chr3:15542254-15542273 None:intergenic 50.0%
CACAGATACAGACACGGACA+CGG - Chr3:15542705-15542724 MsG0380012367.01.T01:intron 50.0%
CTGCAATGTGGTGCTCCATT+TGG - Chr3:15545618-15545637 MsG0380012367.01.T01:intron 50.0%
GCGAGGCAGTTTCTTGGATT+AGG + Chr3:15544686-15544705 None:intergenic 50.0%
TATGAAGCACGGACACTCCT+TGG + Chr3:15542864-15542883 None:intergenic 50.0%
TCCAGGCCATTTCCTTTCGA+TGG + Chr3:15544338-15544357 None:intergenic 50.0%
TGATCTTTCCCTTGCCAGCT+GGG - Chr3:15544438-15544457 MsG0380012367.01.T01:intron 50.0%
TGCAACCCCCACAAATCTCA+AGG - Chr3:15543543-15543562 MsG0380012367.01.T01:intron 50.0%
TGGCCCAGAATATGCGATTG+CGG - Chr3:15544391-15544410 MsG0380012367.01.T01:intron 50.0%
TGGGTCAGATGCAGCTGATA+CGG - Chr3:15542952-15542971 MsG0380012367.01.T01:intron 50.0%
TTGCATAGGGACTGCAGTTC+TGG - Chr3:15545107-15545126 MsG0380012367.01.T01:intron 50.0%
! AAATCTGCAAAAGCGCGCTC+GGG + Chr3:15545592-15545611 None:intergenic 50.0%
! CTCCTTGGATTAGGAGTTCC+CGG + Chr3:15542849-15542868 None:intergenic 50.0%
!! AAGGAGTGTCCGTGCTTCAT+AGG - Chr3:15542863-15542882 MsG0380012367.01.T01:intron 50.0%
!! GGAAAGGAAGTACCTGGATG+AGG + Chr3:15543679-15543698 None:intergenic 50.0%
!! TCTTCCTTTGCTTTGGGACG+AGG - Chr3:15544485-15544504 MsG0380012367.01.T01:intron 50.0%
!! TTGTTTTGCTGTCGGACCAG+GGG + Chr3:15544294-15544313 None:intergenic 50.0%
!!! CTTTGTTTTGCTGTCGGACC+AGG + Chr3:15544296-15544315 None:intergenic 50.0%
ACACGACATGTGTCCGACAC+CGG - Chr3:15542827-15542846 MsG0380012367.01.T01:intron 55.0%
ACTCCAGCGAGGCAGTTTCT+TGG + Chr3:15544692-15544711 None:intergenic 55.0%
ATCGACACGACACCGACACT+TGG + Chr3:15542802-15542821 None:intergenic 55.0%
CACAATCGCAATCTCGGTCG+TGG + Chr3:15543020-15543039 None:intergenic 55.0%
CACCGGGAACTCCTAATCCA+AGG - Chr3:15542844-15542863 MsG0380012367.01.T01:intron 55.0%
CACGACCGAGATTGCGATTG+TGG - Chr3:15543018-15543037 MsG0380012367.01.T01:intron 55.0%
CACTTGGATCCGGAGACAAC+TGG + Chr3:15545302-15545321 None:intergenic 55.0%
CAGACAACTACGACAGACGC+TGG + Chr3:15542211-15542230 None:intergenic 55.0%
CTGATCTTTCCCTTGCCAGC+TGG - Chr3:15544437-15544456 MsG0380012367.01.T01:intron 55.0%
GCACGGACACTCCTTGGATT+AGG + Chr3:15542858-15542877 None:intergenic 55.0%
GCCATCGAAAGGAAATGGCC+TGG - Chr3:15544334-15544353 MsG0380012367.01.T01:CDS 55.0%
GGAAATGGCCTGGACATTGC+AGG - Chr3:15544344-15544363 MsG0380012367.01.T01:CDS 55.0%
GGATTAGGAGTTCCCGGTGT+CGG + Chr3:15542843-15542862 None:intergenic 55.0%
TGTCTTCACTGCCCTCATCC+AGG - Chr3:15543664-15543683 MsG0380012367.01.T01:intron 55.0%
CACGACATGTGTCCGACACC+GGG - Chr3:15542828-15542847 MsG0380012367.01.T01:intron 60.0%
CAGGCCTCGTCCCAAAGCAA+AGG + Chr3:15544492-15544511 None:intergenic 60.0%
CCACGGGATCTGCCATCGAA+AGG - Chr3:15544323-15544342 MsG0380012367.01.T01:CDS 60.0%
CCTTTCGATGGCAGATCCCG+TGG + Chr3:15544326-15544345 None:intergenic 60.0%
! TATGGGGCACTCCCTCTGGA+TGG + Chr3:15544525-15544544 None:intergenic 60.0%
! GGCTTATGGGGCACTCCCTC+TGG + Chr3:15544529-15544548 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
Chr3 gene 15542164 15545701 15542164 ID=MsG0380012367.01;Name=MsG0380012367.01
Chr3 mRNA 15542164 15545701 15542164 ID=MsG0380012367.01.T01;Parent=MsG0380012367.01;Name=MsG0380012367.01.T01;_AED=0.46;_eAED=0.67;_QI=105|0.5|0.4|1|0|0|5|0|169
Chr3 exon 15542164 15542364 15542164 ID=MsG0380012367.01.T01:exon:939;Parent=MsG0380012367.01.T01
Chr3 exon 15542520 15542661 15542520 ID=MsG0380012367.01.T01:exon:938;Parent=MsG0380012367.01.T01
Chr3 exon 15544145 15544227 15544145 ID=MsG0380012367.01.T01:exon:937;Parent=MsG0380012367.01.T01
Chr3 exon 15544301 15544406 15544301 ID=MsG0380012367.01.T01:exon:936;Parent=MsG0380012367.01.T01
Chr3 exon 15545619 15545701 15545619 ID=MsG0380012367.01.T01:exon:935;Parent=MsG0380012367.01.T01
Chr3 five_prime_UTR 15545619 15545701 15545619 ID=MsG0380012367.01.T01:five_prime_utr;Parent=MsG0380012367.01.T01
Chr3 five_prime_UTR 15544385 15544406 15544385 ID=MsG0380012367.01.T01:five_prime_utr;Parent=MsG0380012367.01.T01
Chr3 CDS 15544301 15544384 15544301 ID=MsG0380012367.01.T01:cds;Parent=MsG0380012367.01.T01
Chr3 CDS 15544145 15544227 15544145 ID=MsG0380012367.01.T01:cds;Parent=MsG0380012367.01.T01
Chr3 CDS 15542520 15542661 15542520 ID=MsG0380012367.01.T01:cds;Parent=MsG0380012367.01.T01
Chr3 CDS 15542164 15542364 15542164 ID=MsG0380012367.01.T01:cds;Parent=MsG0380012367.01.T01
Gene Sequence

>MsG0380012367.01.T01

ATGGTGAAGTCTACTGCTAAGGATGCACAAGATCTTCTCCGCACTCTTTGGTCTGCTTATTCTGCAACCCCCACAAATCTCAAGATCATTGATCATTATGTCGTGTTCGCTGTCTTCACTGCCCTCATCCAGGTACTTCCTTTCCTTGCTTCAATTTCTAAAGATCTTACACTTAGATACAATAGTTCTAATCTGCCTAGGTTCATATGCATAAGTTTAGTTCTTGTCTTTCTTATCTATGTTACCATCCTCTATTTTTTGACCAGTTGTCTCCGGATCCAAGTGAATAAAGAGAACAAGGAATTCAAGAAAGGAAATGATGAAACAATGTGTTGGATTGTTTTCTTCACTGATAGATTCTTACAGATGATTGGTATTTCATATACAGGACATTGCACCCGAGCGCGCTTTTGCAGATTTTGTTCTCTGCAATGTGGTGCTCCATTTGGTGATCATGAACTTTCTTGGTTAAGTGGGTTTGTGTGGCTTCATTGTAACAGAACTCATTGA
Protein sequence

>MsG0380012367.01.T01

MVKSTAKDAQDLLRTLWSAYSATPTNLKIIDHYVVFAVFTALIQVLPFLASISKDLTLRYNSSNLPRFICISLVLVFLIYVTILYFLTSCLRIQVNKENKEFKKGNDETMCWIVFFTDRFLQMIGISYTGHCTRARFCRFCSLQCGAPFGDHELSWLSGFVWLHCNRTH*