Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180005497.01.T01 | XP_018459315.1 | 45.397 | 315 | 149 | 6 | 8 | 322 | 29 | 320 | 5.87E-85 | 269 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180005497.01.T01 | A0A6J0LGH1 | 45.397 | 315 | 149 | 6 | 8 | 322 | 29 | 320 | 2.81e-85 | 269 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180005497.01 | MsG0480020505.01 | 0.815400 | 9.426031e-52 | 6.491977e-49 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180005497.01.T01 | MTR_7g062720 | 40.000 | 120 | 65 | 2 | 8 | 126 | 29 | 142 | 1.25e-23 | 95.5 |
| MsG0180005497.01.T01 | MTR_4g037355 | 41.176 | 136 | 60 | 3 | 135 | 270 | 1 | 116 | 9.24e-22 | 89.7 |
| MsG0180005497.01.T01 | MTR_5g061150 | 35.354 | 99 | 64 | 0 | 32 | 130 | 38 | 136 | 3.76e-18 | 80.1 |
| MsG0180005497.01.T01 | MTR_4g112730 | 57.971 | 69 | 28 | 1 | 123 | 191 | 2 | 69 | 5.24e-18 | 78.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 60 sgRNAs with CRISPR-Local
Find 87 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TGGATCAATGGAACAAATTA+AGG | 0.196151 | 1:+92516634 | MsG0180005497.01.T01:CDS |
| AGTCTTATTGCCCTCATTAA+TGG | 0.201947 | 1:+92516614 | MsG0180005497.01.T01:CDS |
| AAAGTTGTGGCGTTTATTTC+AGG | 0.203868 | 1:-92516930 | None:intergenic |
| CAAAAGATCCCAAGATCTTA+TGG | 0.237847 | 1:+92516556 | MsG0180005497.01.T01:CDS |
| GATTCTTGAGGGAGAAGATT+TGG | 0.253340 | 1:+92516437 | MsG0180005497.01.T01:CDS |
| GACAAAATTAAGGCAAGATT+TGG | 0.257188 | 1:+92516579 | MsG0180005497.01.T01:CDS |
| CACGACCTCCACCACGAAAA+TGG | 0.277262 | 1:-92517046 | None:intergenic |
| GATCTTATGGGACAAAATTA+AGG | 0.310971 | 1:+92516569 | MsG0180005497.01.T01:CDS |
| ATTTACTGAATACTCTGATT+TGG | 0.313227 | 1:+92516824 | MsG0180005497.01.T01:CDS |
| ATTCTTGAGGGAGAAGATTT+GGG | 0.333151 | 1:+92516438 | MsG0180005497.01.T01:CDS |
| AGACAGGTAGTGTCATTCTT+TGG | 0.342502 | 1:-92517302 | None:intergenic |
| GGGAGATGTCCCAGAGGAAT+TGG | 0.345733 | 1:+92516458 | MsG0180005497.01.T01:CDS |
| GAAGAGGTTATATGAATTAC+AGG | 0.363099 | 1:+92517069 | MsG0180005497.01.T01:CDS |
| ACTCAATAAGTTGTTGTTCC+AGG | 0.371280 | 1:-92516389 | None:intergenic |
| GATTTCTAGAGGGACCTTCC+TGG | 0.373636 | 1:-92517145 | None:intergenic |
| CATAGCCACCACGTCCTCTT+TGG | 0.374275 | 1:-92516980 | None:intergenic |
| CGTGGTGGCTATGCTCGTTT+TGG | 0.395091 | 1:+92516990 | MsG0180005497.01.T01:CDS |
| ACAAAATTAAGGCAAGATTT+GGG | 0.416566 | 1:+92516580 | MsG0180005497.01.T01:CDS |
| TATAACTGAAAAGGAAAAGT+TGG | 0.422757 | 1:+92516737 | MsG0180005497.01.T01:CDS |
| TGCCCTCATTAATGGATCAA+TGG | 0.432677 | 1:+92516622 | MsG0180005497.01.T01:CDS |
| CGTGGTGGAGGTCGTGGAAG+AGG | 0.449888 | 1:+92517053 | MsG0180005497.01.T01:CDS |
| TCCTGCATGCTTTAGCCCAA+TGG | 0.457473 | 1:-92517202 | None:intergenic |
| AAAAGATCCCAAGATCTTAT+GGG | 0.458748 | 1:+92516557 | MsG0180005497.01.T01:CDS |
| AACCATCAGTTGCGTCCTAC+AGG | 0.459311 | 1:+92516894 | MsG0180005497.01.T01:CDS |
| TGGCAAAACTATAACTGAAA+AGG | 0.467052 | 1:+92516728 | MsG0180005497.01.T01:CDS |
| ATCTGTGGATGGAAAGGAAA+GGG | 0.491819 | 1:+92517256 | MsG0180005497.01.T01:CDS |
| ATCAAGCACCACATTGATGA+TGG | 0.492315 | 1:+92516510 | MsG0180005497.01.T01:CDS |
| TGTTATAGATGCGGAAAGAA+AGG | 0.495634 | 1:+92517179 | MsG0180005497.01.T01:CDS |
| GTTCCATTGATCCATTAATG+AGG | 0.509644 | 1:-92516625 | None:intergenic |
| ATGCGGAAAGAAAGGCCATT+GGG | 0.516896 | 1:+92517187 | MsG0180005497.01.T01:CDS |
| TTTGATTGGTCATATTTGAG+AGG | 0.522357 | 1:-92517092 | None:intergenic |
| CAAAGAATCTGTGGATGGAA+AGG | 0.532926 | 1:+92517250 | MsG0180005497.01.T01:CDS |
| TTCCATTGATCCATTAATGA+GGG | 0.533283 | 1:-92516624 | None:intergenic |
| CGTGGACGAGGTAGAAATCA+TGG | 0.535808 | 1:+92517017 | MsG0180005497.01.T01:CDS |
| GGACTCGATAAGATTCTTGA+GGG | 0.539399 | 1:+92516426 | MsG0180005497.01.T01:CDS |
| GCCATTGGGCTAAAGCATGC+AGG | 0.539769 | 1:+92517201 | MsG0180005497.01.T01:CDS |
| CCCTCTAGAAATCATGATGA+TGG | 0.541875 | 1:+92517155 | MsG0180005497.01.T01:CDS |
| TTCCTGTAGGACGCAACTGA+TGG | 0.562606 | 1:-92516896 | None:intergenic |
| AAAATCAGCAGCCTCAAGAC+AGG | 0.562896 | 1:-92517318 | None:intergenic |
| TATTGAGTATCTTGCATGTG+AGG | 0.576718 | 1:+92516404 | MsG0180005497.01.T01:CDS |
| TTTGGTTGCAGCCCTTCTAG+TGG | 0.582793 | 1:+92516842 | MsG0180005497.01.T01:CDS |
| GATGCGGAAAGAAAGGCCAT+TGG | 0.583841 | 1:+92517186 | MsG0180005497.01.T01:CDS |
| ATTGAGTATCTTGCATGTGA+GGG | 0.584777 | 1:+92516405 | MsG0180005497.01.T01:CDS |
| TTGTGCAATAATTTGATGCA+TGG | 0.588693 | 1:-92516694 | None:intergenic |
| TAAAAGATACAAAGAATCTG+TGG | 0.594054 | 1:+92517241 | MsG0180005497.01.T01:CDS |
| AGATACAAAGAATCTGTGGA+TGG | 0.602731 | 1:+92517245 | MsG0180005497.01.T01:CDS |
| GGGACTCGATAAGATTCTTG+AGG | 0.606176 | 1:+92516425 | MsG0180005497.01.T01:CDS |
| ACGCCCTTGATTAAAAGTTG+TGG | 0.608515 | 1:-92516943 | None:intergenic |
| GCAATATCACTTGAGATGCA+TGG | 0.608661 | 1:-92516779 | None:intergenic |
| TATAATCGCCAAAGAGGACG+TGG | 0.608957 | 1:+92516972 | MsG0180005497.01.T01:CDS |
| ACCATCATCATGATTTCTAG+AGG | 0.616492 | 1:-92517156 | None:intergenic |
| GGATACGACATTGTTCCTGT+AGG | 0.627452 | 1:-92516909 | None:intergenic |
| GGTGGTTATAATCGCCAAAG+AGG | 0.642086 | 1:+92516966 | MsG0180005497.01.T01:CDS |
| CCATCATCATGATTTCTAGA+GGG | 0.647682 | 1:-92517155 | None:intergenic |
| GAATGACACTACCTGTCTTG+AGG | 0.649563 | 1:+92517307 | MsG0180005497.01.T01:CDS |
| AATCTGTGGATGGAAAGGAA+AGG | 0.653361 | 1:+92517255 | MsG0180005497.01.T01:CDS |
| GATGATGGTTGTTATAGATG+CGG | 0.696569 | 1:+92517170 | MsG0180005497.01.T01:CDS |
| AATCGCCAAAGAGGACGTGG+TGG | 0.698905 | 1:+92516975 | MsG0180005497.01.T01:CDS |
| TTTGTAATCCATCATCAATG+TGG | 0.715751 | 1:-92516518 | None:intergenic |
| AGATTTGGGAGATGTCCCAG+AGG | 0.741186 | 1:+92516452 | MsG0180005497.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | ACAAAATTAAGGCAAGATTT+GGG | + | Chr1:92516580-92516599 | MsG0180005497.01.T01:CDS | 25.0% |
| ! | ATTTACTGAATACTCTGATT+TGG | + | Chr1:92516824-92516843 | MsG0180005497.01.T01:CDS | 25.0% |
| ! | CAATCAAACAAAAATCATCA+GGG | + | Chr1:92517107-92517126 | MsG0180005497.01.T01:CDS | 25.0% |
| ! | TAAAAGATACAAAGAATCTG+TGG | + | Chr1:92517241-92517260 | MsG0180005497.01.T01:CDS | 25.0% |
| ! | TATAACTGAAAAGGAAAAGT+TGG | + | Chr1:92516737-92516756 | MsG0180005497.01.T01:CDS | 25.0% |
| !! | ATTGCACAATTAGAATTTTG+TGG | + | Chr1:92516708-92516727 | MsG0180005497.01.T01:CDS | 25.0% |
| !! | TATCTTTTACATAGATACTC+TGG | - | Chr1:92517230-92517249 | None:intergenic | 25.0% |
| !!! | CAACAACAGTTTTATAATCT+TGG | - | Chr1:92516662-92516681 | None:intergenic | 25.0% |
| !!! | TGTTGAAGGAGATTTTAATA+TGG | + | Chr1:92517340-92517359 | MsG0180005497.01.T01:CDS | 25.0% |
| AAAAGATCCCAAGATCTTAT+GGG | + | Chr1:92516557-92516576 | MsG0180005497.01.T01:CDS | 30.0% | |
| AACAAAAATCATCAGGGTAA+AGG | + | Chr1:92517113-92517132 | MsG0180005497.01.T01:CDS | 30.0% | |
| CCAATCAAACAAAAATCATC+AGG | + | Chr1:92517106-92517125 | MsG0180005497.01.T01:CDS | 30.0% | |
| GAAGAGGTTATATGAATTAC+AGG | + | Chr1:92517069-92517088 | MsG0180005497.01.T01:CDS | 30.0% | |
| GACAAAATTAAGGCAAGATT+TGG | + | Chr1:92516579-92516598 | MsG0180005497.01.T01:CDS | 30.0% | |
| GATCTTATGGGACAAAATTA+AGG | + | Chr1:92516569-92516588 | MsG0180005497.01.T01:CDS | 30.0% | |
| TGGATCAATGGAACAAATTA+AGG | + | Chr1:92516634-92516653 | MsG0180005497.01.T01:CDS | 30.0% | |
| TGGCAAAACTATAACTGAAA+AGG | + | Chr1:92516728-92516747 | MsG0180005497.01.T01:CDS | 30.0% | |
| TTCCATTGATCCATTAATGA+GGG | - | Chr1:92516627-92516646 | None:intergenic | 30.0% | |
| TTGTGCAATAATTTGATGCA+TGG | - | Chr1:92516697-92516716 | None:intergenic | 30.0% | |
| TTTGTAATCCATCATCAATG+TGG | - | Chr1:92516521-92516540 | None:intergenic | 30.0% | |
| ! | AATTTTGTCCCATAAGATCT+TGG | - | Chr1:92516568-92516587 | None:intergenic | 30.0% |
| ! | ATTTTGTCCCATAAGATCTT+GGG | - | Chr1:92516567-92516586 | None:intergenic | 30.0% |
| ! | TTTGATTGGTCATATTTGAG+AGG | - | Chr1:92517095-92517114 | None:intergenic | 30.0% |
| !! | CCTGATGATTTTTGTTTGAT+TGG | - | Chr1:92517109-92517128 | None:intergenic | 30.0% |
| AAAGGTAAAAACATCCAGGA+AGG | + | Chr1:92517131-92517150 | MsG0180005497.01.T01:CDS | 35.0% | |
| AAAGTTGTGGCGTTTATTTC+AGG | - | Chr1:92516933-92516952 | None:intergenic | 35.0% | |
| AGATACAAAGAATCTGTGGA+TGG | + | Chr1:92517245-92517264 | MsG0180005497.01.T01:CDS | 35.0% | |
| ATTCTTGAGGGAGAAGATTT+GGG | + | Chr1:92516438-92516457 | MsG0180005497.01.T01:CDS | 35.0% | |
| ATTGAGTATCTTGCATGTGA+GGG | + | Chr1:92516405-92516424 | MsG0180005497.01.T01:CDS | 35.0% | |
| CAAAAGATCCCAAGATCTTA+TGG | + | Chr1:92516556-92516575 | MsG0180005497.01.T01:CDS | 35.0% | |
| GTTCCATTGATCCATTAATG+AGG | - | Chr1:92516628-92516647 | None:intergenic | 35.0% | |
| TATTGAGTATCTTGCATGTG+AGG | + | Chr1:92516404-92516423 | MsG0180005497.01.T01:CDS | 35.0% | |
| TGTTATAGATGCGGAAAGAA+AGG | + | Chr1:92517179-92517198 | MsG0180005497.01.T01:CDS | 35.0% | |
| ! | AACGCCACAACTTTTAATCA+AGG | + | Chr1:92516939-92516958 | MsG0180005497.01.T01:CDS | 35.0% |
| ! | ACCATCATCATGATTTCTAG+AGG | - | Chr1:92517159-92517178 | None:intergenic | 35.0% |
| ! | ACGCCACAACTTTTAATCAA+GGG | + | Chr1:92516940-92516959 | MsG0180005497.01.T01:CDS | 35.0% |
| ! | ATTTTGTTCAGCCACTAGAA+GGG | - | Chr1:92516856-92516875 | None:intergenic | 35.0% |
| ! | CCATCATCATGATTTCTAGA+GGG | - | Chr1:92517158-92517177 | None:intergenic | 35.0% |
| ! | GATGATGGTTGTTATAGATG+CGG | + | Chr1:92517170-92517189 | MsG0180005497.01.T01:CDS | 35.0% |
| ! | TATTTTGTTCAGCCACTAGA+AGG | - | Chr1:92516857-92516876 | None:intergenic | 35.0% |
| ! | TTTTCTTTGCCAATTCCTCT+GGG | - | Chr1:92516470-92516489 | None:intergenic | 35.0% |
| ! | TTTTTCTTTGCCAATTCCTC+TGG | - | Chr1:92516471-92516490 | None:intergenic | 35.0% |
| !! | AGTCTTATTGCCCTCATTAA+TGG | + | Chr1:92516614-92516633 | MsG0180005497.01.T01:CDS | 35.0% |
| AATCTGTGGATGGAAAGGAA+AGG | + | Chr1:92517255-92517274 | MsG0180005497.01.T01:CDS | 40.0% | |
| ACGCCCTTGATTAAAAGTTG+TGG | - | Chr1:92516946-92516965 | None:intergenic | 40.0% | |
| ATCAAGCACCACATTGATGA+TGG | + | Chr1:92516510-92516529 | MsG0180005497.01.T01:CDS | 40.0% | |
| ATCTGTGGATGGAAAGGAAA+GGG | + | Chr1:92517256-92517275 | MsG0180005497.01.T01:CDS | 40.0% | |
| CAAAGAATCTGTGGATGGAA+AGG | + | Chr1:92517250-92517269 | MsG0180005497.01.T01:CDS | 40.0% | |
| GATTCTTGAGGGAGAAGATT+TGG | + | Chr1:92516437-92516456 | MsG0180005497.01.T01:CDS | 40.0% | |
| GCAATATCACTTGAGATGCA+TGG | - | Chr1:92516782-92516801 | None:intergenic | 40.0% | |
| GGACTCGATAAGATTCTTGA+GGG | + | Chr1:92516426-92516445 | MsG0180005497.01.T01:CDS | 40.0% | |
| GGGTAAAGGTAAAAACATCC+AGG | + | Chr1:92517127-92517146 | MsG0180005497.01.T01:CDS | 40.0% | |
| ! | ACAACTTTTAATCAAGGGCG+TGG | + | Chr1:92516945-92516964 | MsG0180005497.01.T01:CDS | 40.0% |
| ! | AGACAGGTAGTGTCATTCTT+TGG | - | Chr1:92517305-92517324 | None:intergenic | 40.0% |
| ! | CCCTCTAGAAATCATGATGA+TGG | + | Chr1:92517155-92517174 | MsG0180005497.01.T01:CDS | 40.0% |
| ! | TGCCCTCATTAATGGATCAA+TGG | + | Chr1:92516622-92516641 | MsG0180005497.01.T01:CDS | 40.0% |
| AAAATCAGCAGCCTCAAGAC+AGG | - | Chr1:92517321-92517340 | None:intergenic | 45.0% | |
| ATGCGGAAAGAAAGGCCATT+GGG | + | Chr1:92517187-92517206 | MsG0180005497.01.T01:CDS | 45.0% | |
| GAATGACACTACCTGTCTTG+AGG | + | Chr1:92517307-92517326 | MsG0180005497.01.T01:CDS | 45.0% | |
| GGATACGACATTGTTCCTGT+AGG | - | Chr1:92516912-92516931 | None:intergenic | 45.0% | |
| GGGACTCGATAAGATTCTTG+AGG | + | Chr1:92516425-92516444 | MsG0180005497.01.T01:CDS | 45.0% | |
| GGTGGTTATAATCGCCAAAG+AGG | + | Chr1:92516966-92516985 | MsG0180005497.01.T01:CDS | 45.0% | |
| TATAATCGCCAAAGAGGACG+TGG | + | Chr1:92516972-92516991 | MsG0180005497.01.T01:CDS | 45.0% | |
| ! | CATGGTCGAAACCATTTTCG+TGG | + | Chr1:92517035-92517054 | MsG0180005497.01.T01:CDS | 45.0% |
| !! | ACTTTTAATCAAGGGCGTGG+TGG | + | Chr1:92516948-92516967 | MsG0180005497.01.T01:CDS | 45.0% |
| !!! | GAGGCTGCTGATTTTGTTGA+AGG | + | Chr1:92517326-92517345 | MsG0180005497.01.T01:CDS | 45.0% |
| AACCATCAGTTGCGTCCTAC+AGG | + | Chr1:92516894-92516913 | MsG0180005497.01.T01:CDS | 50.0% | |
| AGATTTGGGAGATGTCCCAG+AGG | + | Chr1:92516452-92516471 | MsG0180005497.01.T01:CDS | 50.0% | |
| CGTGGACGAGGTAGAAATCA+TGG | + | Chr1:92517017-92517036 | MsG0180005497.01.T01:CDS | 50.0% | |
| GATGCGGAAAGAAAGGCCAT+TGG | + | Chr1:92517186-92517205 | MsG0180005497.01.T01:CDS | 50.0% | |
| TCCTGCATGCTTTAGCCCAA+TGG | - | Chr1:92517205-92517224 | None:intergenic | 50.0% | |
| TTCCTGTAGGACGCAACTGA+TGG | - | Chr1:92516899-92516918 | None:intergenic | 50.0% | |
| TTTGGTTGCAGCCCTTCTAG+TGG | + | Chr1:92516842-92516861 | MsG0180005497.01.T01:CDS | 50.0% | |
| ! | CGAAACCATTTTCGTGGTGG+AGG | + | Chr1:92517041-92517060 | MsG0180005497.01.T01:CDS | 50.0% |
| ! | GATTTCTAGAGGGACCTTCC+TGG | - | Chr1:92517148-92517167 | None:intergenic | 50.0% |
| ! | GGTCGAAACCATTTTCGTGG+TGG | + | Chr1:92517038-92517057 | MsG0180005497.01.T01:CDS | 50.0% |
| !! | TATGCTCGTTTTGGTGGTCG+TGG | + | Chr1:92516999-92517018 | MsG0180005497.01.T01:CDS | 50.0% |
| AATCGCCAAAGAGGACGTGG+TGG | + | Chr1:92516975-92516994 | MsG0180005497.01.T01:CDS | 55.0% | |
| CACGACCTCCACCACGAAAA+TGG | - | Chr1:92517049-92517068 | None:intergenic | 55.0% | |
| CATAGCCACCACGTCCTCTT+TGG | - | Chr1:92516983-92517002 | None:intergenic | 55.0% | |
| GCCATTGGGCTAAAGCATGC+AGG | + | Chr1:92517201-92517220 | MsG0180005497.01.T01:CDS | 55.0% | |
| GGGAGATGTCCCAGAGGAAT+TGG | + | Chr1:92516458-92516477 | MsG0180005497.01.T01:CDS | 55.0% | |
| ! | CGTGGTGGCTATGCTCGTTT+TGG | + | Chr1:92516990-92517009 | MsG0180005497.01.T01:CDS | 55.0% |
| ! | GGTGGCTATGCTCGTTTTGG+TGG | + | Chr1:92516993-92517012 | MsG0180005497.01.T01:CDS | 55.0% |
| !! | CATTTTCGTGGTGGAGGTCG+TGG | + | Chr1:92517047-92517066 | MsG0180005497.01.T01:CDS | 55.0% |
| !! | CGTTTTGGTGGTCGTGGACG+AGG | + | Chr1:92517005-92517024 | MsG0180005497.01.T01:CDS | 60.0% |
| ! | CGTGGTGGAGGTCGTGGAAG+AGG | + | Chr1:92517053-92517072 | MsG0180005497.01.T01:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr1 | gene | 92516390 | 92517367 | 92516390 | ID=MsG0180005497.01;Name=MsG0180005497.01 |
| Chr1 | mRNA | 92516390 | 92517367 | 92516390 | ID=MsG0180005497.01.T01;Parent=MsG0180005497.01;Name=MsG0180005497.01.T01;_AED=0.43;_eAED=0.43;_QI=0|-1|0|1|-1|1|1|0|325 |
| Chr1 | exon | 92516390 | 92517367 | 92516390 | ID=MsG0180005497.01.T01:exon:18981;Parent=MsG0180005497.01.T01 |
| Chr1 | CDS | 92516390 | 92517367 | 92516390 | ID=MsG0180005497.01.T01:cds;Parent=MsG0180005497.01.T01 |
| Gene Sequence |
| Protein sequence |
