AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0180005926.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180005926.01.T01 MTR_1g109660 98.958 96 1 0 38 133 98 193 2.66e-66 199
MsG0180005926.01.T01 MTR_1g109700 64.646 99 34 1 32 129 90 188 3.09e-38 128
MsG0180005926.01.T01 MTR_5g057670 44.000 75 42 0 43 117 106 180 3.06e-17 74.3
MsG0180005926.01.T01 MTR_5g057335 44.000 75 42 0 43 117 106 180 3.06e-17 74.3
MsG0180005926.01.T01 MTR_4g089100 55.224 67 30 0 43 109 101 167 1.59e-16 72.4
MsG0180005926.01.T01 MTR_2g079830 55.882 68 30 0 43 110 102 169 5.54e-11 57.8
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180005926.01.T01 AT1G68220 61.458 96 35 1 38 131 99 194 6.18e-34 117
MsG0180005926.01.T01 AT4G27435 56.250 64 28 0 43 106 102 165 1.36e-17 74.7
MsG0180005926.01.T01 AT1G52910 50.685 73 35 1 43 114 102 174 1.73e-17 74.7
MsG0180005926.01.T01 AT3G15480 52.000 75 31 2 43 116 102 172 8.38e-17 72.8
MsG0180005926.01.T01 AT1G61065 45.679 81 42 1 43 123 101 179 5.99e-16 70.9
MsG0180005926.01.T01 AT1G13380 46.875 64 31 1 43 106 106 166 1.19e-13 64.7

Find 68 sgRNAs with CRISPR-Local

Find 148 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCAAGGCTGACACTGGTTTC+TGG 0.168217 1:-97646889 MsG0180005926.01.T01:CDS
TGTAAAGTGACCATTCTTTC+TGG 0.168738 1:+97646431 None:intergenic
TTTAATATTTATGCCGTAAT+TGG 0.187655 1:-97646670 MsG0180005926.01.T01:three_prime_UTR
CCAATCCAATGCCTTCATTT+TGG 0.194665 1:+97646858 None:intergenic
ACATTCAAATTTCAAGATAT+TGG 0.206552 1:+97646274 None:intergenic
TTACCAAAATGAAGGTGTTT+TGG 0.247519 1:-97646741 MsG0180005926.01.T01:three_prime_UTR
CTTTGTGTGGTAGGCATTCC+TGG 0.250992 1:+97647036 None:intergenic
CGCAAAGGTGTGTTTGCTGC+TGG 0.268553 1:-97646968 MsG0180005926.01.T01:CDS
CTTCAGGGCTTATCAGATTC+AGG 0.297517 1:+97646818 None:intergenic
CTTTACTAAATTGATACATT+TGG 0.335154 1:+97646398 None:intergenic
TTTCAAGCATTCACCAATTA+CGG 0.344003 1:+97646657 None:intergenic
AAGTGGAAAACTCGACTAAA+AGG 0.357029 1:+97646455 None:intergenic
CAATAGCAACACTTTCAAAC+TGG 0.362205 1:+97646577 None:intergenic
GTTGAAAGCAGTAAACCTTC+AGG 0.380221 1:+97646802 None:intergenic
CTTGACCTTGTTCTCTATGT+TGG 0.382330 1:-97646939 MsG0180005926.01.T01:CDS
ATCAATAAAGCCAGGATGGC+TGG 0.387283 1:-97647735 MsG0180005926.01.T01:CDS
GCAGAGGCGTGCTTGTTAGC+AGG 0.394015 1:-97647064 MsG0180005926.01.T01:CDS
ACTTGCACCTCCAGCCATCC+TGG 0.407077 1:+97647725 None:intergenic
GCAAAGGTGTGTTTGCTGCT+GGG 0.415418 1:-97646967 MsG0180005926.01.T01:CDS
CAAGGCTGACACTGGTTTCT+GGG 0.415808 1:-97646888 MsG0180005926.01.T01:CDS
ATCCTGGCTTTATTGATGTA+TGG 0.421677 1:+97647741 None:intergenic
TGACCATTCTTTCTGGAAAG+TGG 0.424788 1:+97646438 None:intergenic
TTTCCACTTTCCAGAAAGAA+TGG 0.435932 1:-97646441 MsG0180005926.01.T01:three_prime_UTR
ACAATATATAGATAAATAGA+TGG 0.446395 1:-97646635 MsG0180005926.01.T01:three_prime_UTR
GTTTACTGCTTTCAACATGC+TGG 0.447801 1:-97646795 MsG0180005926.01.T01:three_prime_UTR
CAAAGGTGTGTTTGCTGCTG+GGG 0.452357 1:-97646966 MsG0180005926.01.T01:CDS
AATAGATGGTAGTGGTATGG+TGG 0.454376 1:-97646621 MsG0180005926.01.T01:three_prime_UTR
ATTAGATGCACTAAAGCAAA+TGG 0.470320 1:+97646534 None:intergenic
CAATGTGTTCAAAACCTTGT+TGG 0.472667 1:-97646702 MsG0180005926.01.T01:three_prime_UTR
AGTGTTGCTATTGAAACAAA+TGG 0.479016 1:-97646567 MsG0180005926.01.T01:three_prime_UTR
GCTTGTTAGCAGGCTCTGCC+AGG 0.484157 1:-97647054 MsG0180005926.01.T01:CDS
TGGTCGAACTCGGGAGTTTG+AGG 0.499771 1:-97647694 MsG0180005926.01.T01:CDS
TTGAAAGCAGTAAACCTTCA+GGG 0.502427 1:+97646803 None:intergenic
ATAGATGGTAGTGGTATGGT+GGG 0.504617 1:-97646620 MsG0180005926.01.T01:three_prime_UTR
AAGCACCAAAATGAAGGCAT+TGG 0.516097 1:-97646863 MsG0180005926.01.T01:CDS
GTGCACGATGTGGTCGAACT+CGG 0.517827 1:-97647704 MsG0180005926.01.T01:CDS
TTTACTGCTTTCAACATGCT+GGG 0.519387 1:-97646794 MsG0180005926.01.T01:three_prime_UTR
ATAAATAGATGGTAGTGGTA+TGG 0.540818 1:-97646624 MsG0180005926.01.T01:three_prime_UTR
TGGGAGAAGCACCAAAATGA+AGG 0.543939 1:-97646869 MsG0180005926.01.T01:CDS
CCAAAATGAAGGCATTGGAT+TGG 0.547050 1:-97646858 MsG0180005926.01.T01:CDS
TGCACGATGTGGTCGAACTC+GGG 0.549275 1:-97647703 MsG0180005926.01.T01:CDS
CCCTCGGTACTTTGTGTGGT+AGG 0.550996 1:+97647027 None:intergenic
CCAGAAACCAGTGTCAGCCT+TGG 0.551041 1:+97646889 None:intergenic
GATGCACTAAAGCAAATGGT+TGG 0.551676 1:+97646538 None:intergenic
AATTCCCTCGGTACTTTGTG+TGG 0.559584 1:+97647023 None:intergenic
TATAGATAAATAGATGGTAG+TGG 0.562653 1:-97646629 MsG0180005926.01.T01:three_prime_UTR
TTCCATACATCAATAAAGCC+AGG 0.564779 1:-97647743 MsG0180005926.01.T01:CDS
GCACACGCCAAGGCTGACAC+TGG 0.573418 1:-97646896 MsG0180005926.01.T01:CDS
GCTCATAGGATCCATGGCCA+TGG 0.574521 1:-97647766 None:intergenic
AGATGGTAGTGGTATGGTGG+GGG 0.575873 1:-97646618 MsG0180005926.01.T01:three_prime_UTR
CTGAATCTGATAAGCCCTGA+AGG 0.582169 1:-97646817 MsG0180005926.01.T01:exon
GTACTATTGGGCACACGCCA+AGG 0.584073 1:-97646906 MsG0180005926.01.T01:CDS
AGAAGCCAACATAGAGAACA+AGG 0.611678 1:+97646934 None:intergenic
TTTATTGATGTATGGAACCA+TGG 0.615525 1:+97647749 None:intergenic
GATGTATGGAACCATGGCCA+TGG 0.618917 1:+97647755 None:intergenic
TAGATGGTAGTGGTATGGTG+GGG 0.627375 1:-97646619 MsG0180005926.01.T01:three_prime_UTR
CGTGTGTCACAAAATTCCCT+CGG 0.630799 1:+97647011 None:intergenic
CAACATAGAGAACAAGGTCA+AGG 0.636116 1:+97646940 None:intergenic
TCGAACTCGGGAGTTTGAGG+TGG 0.644246 1:-97647691 MsG0180005926.01.T01:CDS
TCTTGCGCTACACTTCGCAA+AGG 0.645552 1:-97646983 MsG0180005926.01.T01:CDS
GGATTGGCCACACACAATCA+CGG 0.646265 1:-97646842 MsG0180005926.01.T01:CDS
ATACATCAATAAAGCCAGGA+TGG 0.648184 1:-97647739 MsG0180005926.01.T01:CDS
TTCAGGACCGTGATTGTGTG+TGG 0.660100 1:+97646835 None:intergenic
AATAAAGCCAGGATGGCTGG+AGG 0.681712 1:-97647732 MsG0180005926.01.T01:CDS
GCCTACCACACAAAGTACCG+AGG 0.682833 1:-97647028 MsG0180005926.01.T01:CDS
CCTACCACACAAAGTACCGA+GGG 0.685131 1:-97647027 MsG0180005926.01.T01:CDS
GGAGGTGCAAGTGCACGATG+TGG 0.693827 1:-97647714 MsG0180005926.01.T01:CDS
TTAAATATACTCTACCAACA+AGG 0.709057 1:+97646688 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAGCAAAAAATAAATATGA+TGG + Chr1:97646626-97646645 None:intergenic 15.0%
!! AAGCAAAAAATAAATATGAT+GGG + Chr1:97646625-97646644 None:intergenic 15.0%
!! ACAATATATAGATAAATAGA+TGG - Chr1:97647378-97647397 MsG0180005926.01.T01:intron 15.0%
!! ACATTCAAATTTCAAGATAT+TGG + Chr1:97647742-97647761 None:intergenic 20.0%
!! CTAAGTAATAATACAAAAGA+AGG + Chr1:97646686-97646705 None:intergenic 20.0%
!! CTTATACATATAAACACAAA+AGG + Chr1:97646757-97646776 None:intergenic 20.0%
!! CTTTACTAAATTGATACATT+TGG + Chr1:97647618-97647637 None:intergenic 20.0%
!! TTTAATATTTATGCCGTAAT+TGG - Chr1:97647343-97647362 MsG0180005926.01.T01:intron 20.0%
!!! TTACCAAGATTTTTTTATCA+CGG + Chr1:97647515-97647534 None:intergenic 20.0%
! AATCATCATTATTTCACTTC+AGG + Chr1:97646888-97646907 None:intergenic 25.0%
! ATTATTGTTGCTGTAAAATC+AGG - Chr1:97646911-97646930 MsG0180005926.01.T01:CDS 25.0%
! TATAGATAAATAGATGGTAG+TGG - Chr1:97647384-97647403 MsG0180005926.01.T01:intron 25.0%
! TCTCATAATGTTACAAAAAG+AGG + Chr1:97646521-97646540 None:intergenic 25.0%
! TTAAATATACTCTACCAACA+AGG + Chr1:97647328-97647347 None:intergenic 25.0%
! TTACCGTGATAAAAAAATCT+TGG - Chr1:97647509-97647528 MsG0180005926.01.T01:intron 25.0%
! TTAGTTTACTAGTGATTTAG+TGG - Chr1:97646702-97646721 MsG0180005926.01.T01:three_prime_UTR 25.0%
! TTCACTAATTAGTAGAACTA+GGG - Chr1:97646559-97646578 MsG0180005926.01.T01:three_prime_UTR 25.0%
! TTTCACTAATTAGTAGAACT+AGG - Chr1:97646558-97646577 MsG0180005926.01.T01:three_prime_UTR 25.0%
!!! ATTGCTTTTTACCAAAATGA+AGG - Chr1:97647264-97647283 MsG0180005926.01.T01:intron 25.0%
!!! TAGACTTCTATATGTTTTTG+AGG - Chr1:97646645-97646664 MsG0180005926.01.T01:three_prime_UTR 25.0%
ACACAAAAAGATAATGAACG+AGG + Chr1:97647682-97647701 None:intergenic 30.0%
ATGGTCATGTGAAAGAAAAT+AGG + Chr1:97646493-97646512 None:intergenic 30.0%
ATTAGATGCACTAAAGCAAA+TGG + Chr1:97647482-97647501 None:intergenic 30.0%
CACAAAAAGATAATGAACGA+GGG + Chr1:97647681-97647700 None:intergenic 30.0%
GTTACAAAAAGAGGAAGTAA+TGG + Chr1:97646512-97646531 None:intergenic 30.0%
TAATTAGTGAAAGATCCTGA+GGG + Chr1:97646550-97646569 None:intergenic 30.0%
TCGAGATTCTATGTAAGATT+GGG + Chr1:97646850-97646869 None:intergenic 30.0%
TGGTCATGTGAAAGAAAATA+GGG + Chr1:97646492-97646511 None:intergenic 30.0%
TTTATTGATGTATGGAACCA+TGG + Chr1:97646267-97646286 None:intergenic 30.0%
TTTCAAGCATTCACCAATTA+CGG + Chr1:97647359-97647378 None:intergenic 30.0%
! AGTGTTGCTATTGAAACAAA+TGG - Chr1:97647446-97647465 MsG0180005926.01.T01:intron 30.0%
! GGCTTCTATTTTGTACTATT+GGG - Chr1:97647095-97647114 MsG0180005926.01.T01:intron 30.0%
! TCGTTCATTATCTTTTTGTG+TGG - Chr1:97647681-97647700 MsG0180005926.01.T01:CDS 30.0%
! TGGCTTCTATTTTGTACTAT+TGG - Chr1:97647094-97647113 MsG0180005926.01.T01:intron 30.0%
!! ATAAATAGATGGTAGTGGTA+TGG - Chr1:97647389-97647408 MsG0180005926.01.T01:intron 30.0%
!! CTATATGTTTTTGAGGATGA+GGG - Chr1:97646652-97646671 MsG0180005926.01.T01:three_prime_UTR 30.0%
!! TCTATATGTTTTTGAGGATG+AGG - Chr1:97646651-97646670 MsG0180005926.01.T01:three_prime_UTR 30.0%
!!! TACCAAAATGAAGGTGTTTT+GGG - Chr1:97647273-97647292 MsG0180005926.01.T01:intron 30.0%
!!! TTACCAAAATGAAGGTGTTT+TGG - Chr1:97647272-97647291 MsG0180005926.01.T01:intron 30.0%
AAAAAGATAATGAACGAGGG+CGG + Chr1:97647678-97647697 None:intergenic 35.0%
AACTGTAAGATCCTACAGAA+GGG + Chr1:97646790-97646809 None:intergenic 35.0%
AAGTGGAAAACTCGACTAAA+AGG + Chr1:97647561-97647580 None:intergenic 35.0%
AATTAGTGAAAGATCCTGAG+GGG + Chr1:97646549-97646568 None:intergenic 35.0%
AGATTCTATGTAAGATTGGG+AGG + Chr1:97646847-97646866 None:intergenic 35.0%
ATACATCAATAAAGCCAGGA+TGG - Chr1:97646274-97646293 MsG0180005926.01.T01:three_prime_UTR 35.0%
ATGTGAAAGAAAATAGGGGA+AGG + Chr1:97646487-97646506 None:intergenic 35.0%
ATTCTATGTAAGATTGGGAG+GGG + Chr1:97646845-97646864 None:intergenic 35.0%
CAATAGCAACACTTTCAAAC+TGG + Chr1:97647439-97647458 None:intergenic 35.0%
CAATGTGTTCAAAACCTTGT+TGG - Chr1:97647311-97647330 MsG0180005926.01.T01:intron 35.0%
CCAAAAAAAGATTTGGGAGA+AGG + Chr1:97647653-97647672 None:intergenic 35.0%
CTAATTAGTGAAAGATCCTG+AGG + Chr1:97646551-97646570 None:intergenic 35.0%
CTCGAGATTCTATGTAAGAT+TGG + Chr1:97646851-97646870 None:intergenic 35.0%
GATTCTATGTAAGATTGGGA+GGG + Chr1:97646846-97646865 None:intergenic 35.0%
GCGGTTCCAAAAAAAGATTT+GGG + Chr1:97647659-97647678 None:intergenic 35.0%
GGTCATGTGAAAGAAAATAG+GGG + Chr1:97646491-97646510 None:intergenic 35.0%
TGTAAAGTGACCATTCTTTC+TGG + Chr1:97647585-97647604 None:intergenic 35.0%
TTCCATACATCAATAAAGCC+AGG - Chr1:97646270-97646289 MsG0180005926.01.T01:three_prime_UTR 35.0%
TTGAAAGCAGTAAACCTTCA+GGG + Chr1:97647213-97647232 None:intergenic 35.0%
TTTACTGCTTTCAACATGCT+GGG - Chr1:97647219-97647238 MsG0180005926.01.T01:intron 35.0%
TTTCCACTTTCCAGAAAGAA+TGG - Chr1:97647572-97647591 MsG0180005926.01.T01:intron 35.0%
! TAGTAGAACTAGGGTTTTTC+CGG - Chr1:97646568-97646587 MsG0180005926.01.T01:three_prime_UTR 35.0%
!! ATCCTGGCTTTATTGATGTA+TGG + Chr1:97646275-97646294 None:intergenic 35.0%
!!! ACCAAAATGAAGGTGTTTTG+GGG - Chr1:97647274-97647293 MsG0180005926.01.T01:intron 35.0%
!!! CCTTCTCCCAAATCTTTTTT+TGG - Chr1:97647650-97647669 MsG0180005926.01.T01:intron 35.0%
!!! TAAAATCAGGACCGGTTTTT+TGG - Chr1:97646924-97646943 MsG0180005926.01.T01:CDS 35.0%
AGAAGCCAACATAGAGAACA+AGG + Chr1:97647082-97647101 None:intergenic 40.0%
ATATGAACGCCATCACATAG+AGG + Chr1:97646391-97646410 None:intergenic 40.0%
CAACATAGAGAACAAGGTCA+AGG + Chr1:97647076-97647095 None:intergenic 40.0%
CAACTGTAAGATCCTACAGA+AGG + Chr1:97646791-97646810 None:intergenic 40.0%
CTTGACCTTGTTCTCTATGT+TGG - Chr1:97647074-97647093 MsG0180005926.01.T01:CDS 40.0%
GATGCACTAAAGCAAATGGT+TGG + Chr1:97647478-97647497 None:intergenic 40.0%
GGCGGTTCCAAAAAAAGATT+TGG + Chr1:97647660-97647679 None:intergenic 40.0%
GGTTGAAAGATCATAAGTCG+AGG + Chr1:97646823-97646842 None:intergenic 40.0%
GTTGAAAGCAGTAAACCTTC+AGG + Chr1:97647214-97647233 None:intergenic 40.0%
GTTTACTGCTTTCAACATGC+TGG - Chr1:97647218-97647237 MsG0180005926.01.T01:intron 40.0%
TGAATGGGAAAAGAGAGCTT+GGG - Chr1:97646591-97646610 MsG0180005926.01.T01:three_prime_UTR 40.0%
TGTTGCTGTAAAATCAGGAC+CGG - Chr1:97646916-97646935 MsG0180005926.01.T01:CDS 40.0%
TTCTATGTAAGATTGGGAGG+GGG + Chr1:97646844-97646863 None:intergenic 40.0%
! AAGCACCAAAATGAAGGCAT+TGG - Chr1:97647150-97647169 MsG0180005926.01.T01:intron 40.0%
! CCAATCCAATGCCTTCATTT+TGG + Chr1:97647158-97647177 None:intergenic 40.0%
! TGACCATTCTTTCTGGAAAG+TGG + Chr1:97647578-97647597 None:intergenic 40.0%
!! AATAAATATGATGGGCCTCG+TGG + Chr1:97646617-97646636 None:intergenic 40.0%
!! AATAGATGGTAGTGGTATGG+TGG - Chr1:97647392-97647411 MsG0180005926.01.T01:intron 40.0%
!! ATAGATGGTAGTGGTATGGT+GGG - Chr1:97647393-97647412 MsG0180005926.01.T01:intron 40.0%
!! CCAAAATGAAGGCATTGGAT+TGG - Chr1:97647155-97647174 MsG0180005926.01.T01:intron 40.0%
!!! CCAAAATGAAGGTGTTTTGG+GGG - Chr1:97647275-97647294 MsG0180005926.01.T01:intron 40.0%
!!! TTGCAAGCTAGCTTTTGCAA+CGG - Chr1:97646347-97646366 MsG0180005926.01.T01:three_prime_UTR 40.0%
AATTCCCTCGGTACTTTGTG+TGG + Chr1:97646993-97647012 None:intergenic 45.0%
ATCAATAAAGCCAGGATGGC+TGG - Chr1:97646278-97646297 MsG0180005926.01.T01:three_prime_UTR 45.0%
ATGTATGGAACCATGGCCAT+GGG + Chr1:97646260-97646279 None:intergenic 45.0%
ATGTGAGAACTGCGTCAGAA+TGG + Chr1:97646426-97646445 None:intergenic 45.0%
ATTATGAGAGTTGACCCCTC+AGG - Chr1:97646532-97646551 MsG0180005926.01.T01:three_prime_UTR 45.0%
CGTGTGTCACAAAATTCCCT+CGG + Chr1:97647005-97647024 None:intergenic 45.0%
CTGAATCTGATAAGCCCTGA+AGG - Chr1:97647196-97647215 MsG0180005926.01.T01:intron 45.0%
CTTCAGGGCTTATCAGATTC+AGG + Chr1:97647198-97647217 None:intergenic 45.0%
GTGAATGGGAAAAGAGAGCT+TGG - Chr1:97646590-97646609 MsG0180005926.01.T01:three_prime_UTR 45.0%
TGTGAGAACTGCGTCAGAAT+GGG + Chr1:97646425-97646444 None:intergenic 45.0%
! ACTAGGGTTTTTCCGGTGAA+TGG - Chr1:97646575-97646594 MsG0180005926.01.T01:three_prime_UTR 45.0%
! AGCTCTCTTTTCCCATTCAC+CGG + Chr1:97646590-97646609 None:intergenic 45.0%
! CCCCCAAAACACCTTCATTT+TGG + Chr1:97647278-97647297 None:intergenic 45.0%
! CTAGGGTTTTTCCGGTGAAT+GGG - Chr1:97646576-97646595 MsG0180005926.01.T01:three_prime_UTR 45.0%
! GCACAATTTTGCCCTTCTGT+AGG - Chr1:97646776-97646795 MsG0180005926.01.T01:three_prime_UTR 45.0%
!! TAGATGGTAGTGGTATGGTG+GGG - Chr1:97647394-97647413 MsG0180005926.01.T01:intron 45.0%
!! TGGGAGAAGCACCAAAATGA+AGG - Chr1:97647144-97647163 MsG0180005926.01.T01:intron 45.0%
!!! TGCAAGCTAGCTTTTGCAAC+GGG - Chr1:97646348-97646367 MsG0180005926.01.T01:three_prime_UTR 45.0%
AAGAAGGTGAGCAGATCGCA+AGG + Chr1:97646459-97646478 None:intergenic 50.0%
AATAAAGCCAGGATGGCTGG+AGG - Chr1:97646281-97646300 MsG0180005926.01.T01:three_prime_UTR 50.0%
AGAAGGTGAGCAGATCGCAA+GGG + Chr1:97646458-97646477 None:intergenic 50.0%
ATAGGGGAAGGCAGAGAAGA+AGG + Chr1:97646475-97646494 None:intergenic 50.0%
CTTTGTGTGGTAGGCATTCC+TGG + Chr1:97646980-97646999 None:intergenic 50.0%
GATGTATGGAACCATGGCCA+TGG + Chr1:97646261-97646280 None:intergenic 50.0%
TCTTGCGCTACACTTCGCAA+AGG - Chr1:97647030-97647049 MsG0180005926.01.T01:CDS 50.0%
TGAACGCCATCACATAGAGG+AGG + Chr1:97646388-97646407 None:intergenic 50.0%
TTCAGGACCGTGATTGTGTG+TGG + Chr1:97647181-97647200 None:intergenic 50.0%
! CAAAGGTGTGTTTGCTGCTG+GGG - Chr1:97647047-97647066 MsG0180005926.01.T01:CDS 50.0%
! GCAAAGGTGTGTTTGCTGCT+GGG - Chr1:97647046-97647065 MsG0180005926.01.T01:CDS 50.0%
!! AGATGGTAGTGGTATGGTGG+GGG - Chr1:97647395-97647414 MsG0180005926.01.T01:intron 50.0%
!! CAAGGCTGACACTGGTTTCT+GGG - Chr1:97647125-97647144 MsG0180005926.01.T01:intron 50.0%
!! CCTACCACACAAAGTACCGA+GGG - Chr1:97646986-97647005 MsG0180005926.01.T01:CDS 50.0%
!! GCTTTTGCAACGGGCTTTCA+CGG - Chr1:97646357-97646376 MsG0180005926.01.T01:three_prime_UTR 50.0%
!! GGATTGGCCACACACAATCA+CGG - Chr1:97647171-97647190 MsG0180005926.01.T01:intron 50.0%
!!! GAAGGTGTTTTGGGGGATGA+AGG - Chr1:97647282-97647301 MsG0180005926.01.T01:intron 50.0%
!!! GTGTTTTGGGGGATGAAGGA+AGG - Chr1:97647286-97647305 MsG0180005926.01.T01:intron 50.0%
AAAAGAGAGCTTGGGCCACG+AGG - Chr1:97646599-97646618 MsG0180005926.01.T01:three_prime_UTR 55.0%
AACTGCGTCAGAATGGGTGG+CGG + Chr1:97646419-97646438 None:intergenic 55.0%
ACGCCATCACATAGAGGAGG+AGG + Chr1:97646385-97646404 None:intergenic 55.0%
CCAGAAACCAGTGTCAGCCT+TGG + Chr1:97647127-97647146 None:intergenic 55.0%
CCCTCGGTACTTTGTGTGGT+AGG + Chr1:97646989-97647008 None:intergenic 55.0%
CGCCATCACATAGAGGAGGA+GGG + Chr1:97646384-97646403 None:intergenic 55.0%
CGCCTCTGCTGCCAAAAAAC+CGG + Chr1:97646938-97646957 None:intergenic 55.0%
GAAGGTGAGCAGATCGCAAG+GGG + Chr1:97646457-97646476 None:intergenic 55.0%
GAGAACTGCGTCAGAATGGG+TGG + Chr1:97646422-97646441 None:intergenic 55.0%
GTACTATTGGGCACACGCCA+AGG - Chr1:97647107-97647126 MsG0180005926.01.T01:intron 55.0%
GTCCCTCCTCCTCTATGTGA+TGG - Chr1:97646379-97646398 MsG0180005926.01.T01:three_prime_UTR 55.0%
GTGCACGATGTGGTCGAACT+CGG - Chr1:97646309-97646328 MsG0180005926.01.T01:three_prime_UTR 55.0%
TCGAACTCGGGAGTTTGAGG+TGG - Chr1:97646322-97646341 MsG0180005926.01.T01:three_prime_UTR 55.0%
TGCACGATGTGGTCGAACTC+GGG - Chr1:97646310-97646329 MsG0180005926.01.T01:three_prime_UTR 55.0%
TGGTCGAACTCGGGAGTTTG+AGG - Chr1:97646319-97646338 MsG0180005926.01.T01:three_prime_UTR 55.0%
! CGCAAAGGTGTGTTTGCTGC+TGG - Chr1:97647045-97647064 MsG0180005926.01.T01:CDS 55.0%
!! CCAAGGCTGACACTGGTTTC+TGG - Chr1:97647124-97647143 MsG0180005926.01.T01:intron 55.0%
!! GCCTACCACACAAAGTACCG+AGG - Chr1:97646985-97647004 MsG0180005926.01.T01:CDS 55.0%
!!! GACCGGTTTTTTGGCAGCAG+AGG - Chr1:97646933-97646952 MsG0180005926.01.T01:CDS 55.0%
ACTTGCACCTCCAGCCATCC+TGG + Chr1:97646291-97646310 None:intergenic 60.0%
! GCAGAGGCGTGCTTGTTAGC+AGG - Chr1:97646949-97646968 MsG0180005926.01.T01:CDS 60.0%
! GCTTGTTAGCAGGCTCTGCC+AGG - Chr1:97646959-97646978 MsG0180005926.01.T01:CDS 60.0%
! GGAGGTGCAAGTGCACGATG+TGG - Chr1:97646299-97646318 MsG0180005926.01.T01:three_prime_UTR 60.0%
GCACACGCCAAGGCTGACAC+TGG - Chr1:97647117-97647136 MsG0180005926.01.T01:intron 65.0%
GGTGAGCAGATCGCAAGGGG+CGG + Chr1:97646454-97646473 None:intergenic 65.0%
Chromosome Type Strat End Strand Name
Chr1 gene 97646260 97647775 97646260 ID=MsG0180005926.01;Name=MsG0180005926.01
Chr1 mRNA 97646260 97647775 97646260 ID=MsG0180005926.01.T01;Parent=MsG0180005926.01;Name=MsG0180005926.01.T01;_AED=0.43;_eAED=0.50;_QI=0|0|0.5|1|1|0.5|2|560|133
Chr1 exon 97646260 97647102 97646260 ID=MsG0180005926.01.T01:exon:50724;Parent=MsG0180005926.01.T01
Chr1 exon 97647657 97647775 97647657 ID=MsG0180005926.01.T01:exon:50723;Parent=MsG0180005926.01.T01
Chr1 CDS 97647657 97647775 97647657 ID=MsG0180005926.01.T01:cds;Parent=MsG0180005926.01.T01
Chr1 CDS 97646820 97647102 97646820 ID=MsG0180005926.01.T01:cds;Parent=MsG0180005926.01.T01
Chr1 three_prime_UTR 97646260 97646819 97646260 ID=MsG0180005926.01.T01:three_prime_utr;Parent=MsG0180005926.01.T01
Gene Sequence

>MsG0180005926.01.T01

ATGGCCATGGTTCCATACATCAATAAAGCCAGGATGGCTGGAGGTGCAAGTGCACGATGTGGTCGAACTCGGGAGTTTGAGGTGGAATTGCAAGCTAGCTTTTGCAACGGGCTTTCACGGACCGGTTTTTTGGCAGCAGAGGCGTGCTTGTTAGCAGGCTCTGCCAGGAATGCCTACCACACAAAGTACCGAGGGAATTTTGTGACACACGACTTGTCTTGCGCTACACTTCGCAAAGGTGTGTTTGCTGCTGGGGCTGCCTTGACCTTGTTCTCTATGTTGGCTTCTATTTTGTACTATTGGGCACACGCCAAGGCTGACACTGGTTTCTGGGAGAAGCACCAAAATGAAGGCATTGGATTGGCCACACACAATCACGGTCCTGAATCTGATAAGCCCTGA
Protein sequence

>MsG0180005926.01.T01

MAMVPYINKARMAGGASARCGRTREFEVELQASFCNGLSRTGFLAAEACLLAGSARNAYHTKYRGNFVTHDLSCATLRKGVFAAGAALTLFSMLASILYYWAHAKADTGFWEKHQNEGIGLATHNHGPESDKP*