Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180005927.01.T01 | AFK38730.1 | 98 | 100 | 2 | 0 | 1 | 100 | 1 | 100 | 3.37E-62 | 197 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180005927.01.T01 | I3SEN8 | 98.000 | 100 | 2 | 0 | 1 | 100 | 1 | 100 | 1.61e-62 | 197 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0080048761.01 | MsG0180005927.01 | -0.803857 | 2.852921e-49 | 1.449242e-46 |
| MsG0180004997.01 | MsG0180005927.01 | 0.802256 | 6.115588e-49 | 2.981875e-46 |
| MsG0180005926.01 | MsG0180005927.01 | 0.826127 | 3.216632e-54 | 2.990042e-51 |
| MsG0180005927.01 | MsG0180006144.01 | 0.821334 | 4.266230e-53 | 3.460859e-50 |
| MsG0180005927.01 | MsG0180006220.01 | 0.810769 | 9.774295e-51 | 5.944304e-48 |
| MsG0180005927.01 | MsG0280007763.01 | 0.811336 | 7.366514e-51 | 4.547440e-48 |
| MsG0180005927.01 | MsG0280010586.01 | 0.804021 | 2.638675e-49 | 1.346036e-46 |
| MsG0180005927.01 | MsG0280011304.01 | 0.811913 | 5.518061e-51 | 3.458792e-48 |
| MsG0180005927.01 | MsG0380015533.01 | 0.826196 | 3.097949e-54 | 2.885404e-51 |
| MsG0180005927.01 | MsG0380016004.01 | 0.830923 | 2.233361e-55 | 2.386998e-52 |
| MsG0180005927.01 | MsG0380016191.01 | -0.811494 | 6.806910e-51 | 4.219949e-48 |
| MsG0180005927.01 | MsG0380017085.01 | 0.813430 | 2.569993e-51 | 1.677948e-48 |
| MsG0180005927.01 | MsG0480018603.01 | 0.810643 | 1.041160e-50 | 6.310344e-48 |
| MsG0180005927.01 | MsG0480021280.01 | 0.801168 | 1.022630e-48 | 4.850605e-46 |
| MsG0180005927.01 | MsG0480021764.01 | 0.801888 | 7.278763e-49 | 3.516093e-46 |
| MsG0180005927.01 | MsG0480022340.01 | -0.800293 | 1.542451e-48 | 7.156021e-46 |
| MsG0180005927.01 | MsG0580029925.01 | 0.804776 | 1.836370e-49 | 9.551622e-47 |
| MsG0180005927.01 | MsG0780041419.01 | 0.804434 | 2.164629e-49 | 1.115939e-46 |
| MsG0180005927.01 | MsG0880043905.01 | 0.839437 | 1.585203e-57 | 2.189178e-54 |
| MsG0180005927.01 | MsG0880046930.01 | 0.803326 | 3.677102e-49 | 1.842458e-46 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180005927.01.T01 | MTR_1g109660 | 98.000 | 100 | 2 | 0 | 1 | 100 | 1 | 100 | 4.46e-66 | 197 |
| MsG0180005927.01.T01 | MTR_8g027455 | 90.164 | 61 | 6 | 0 | 32 | 92 | 319 | 379 | 4.52e-34 | 121 |
| MsG0180005927.01.T01 | MTR_7g114270 | 76.712 | 73 | 12 | 1 | 25 | 97 | 2 | 69 | 7.38e-34 | 112 |
| MsG0180005927.01.T01 | MTR_1g109700 | 55.000 | 100 | 43 | 1 | 1 | 100 | 1 | 98 | 8.16e-34 | 115 |
| MsG0180005927.01.T01 | MTR_1g109690 | 52.000 | 100 | 46 | 1 | 1 | 100 | 1 | 98 | 1.50e-28 | 99.4 |
| MsG0180005927.01.T01 | MTR_2g079830 | 44.330 | 97 | 54 | 0 | 4 | 100 | 3 | 99 | 5.52e-27 | 98.2 |
| MsG0180005927.01.T01 | MTR_5g057670 | 40.206 | 97 | 58 | 0 | 4 | 100 | 7 | 103 | 1.89e-20 | 81.3 |
| MsG0180005927.01.T01 | MTR_5g057335 | 40.206 | 97 | 58 | 0 | 4 | 100 | 7 | 103 | 1.89e-20 | 81.3 |
| MsG0180005927.01.T01 | MTR_4g089100 | 37.000 | 100 | 61 | 2 | 1 | 100 | 1 | 98 | 6.27e-17 | 72.0 |
| MsG0180005927.01.T01 | MTR_8g098920 | 91.429 | 35 | 3 | 0 | 46 | 80 | 100 | 134 | 1.75e-15 | 67.4 |
| MsG0180005927.01.T01 | MTR_4g094850 | 71.429 | 49 | 10 | 1 | 32 | 80 | 79 | 123 | 1.65e-14 | 64.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0180005927.01.T01 | AT1G68220 | 63.366 | 101 | 36 | 1 | 1 | 100 | 1 | 101 | 1.60e-42 | 138 |
| MsG0180005927.01.T01 | AT3G15480 | 46.000 | 100 | 53 | 1 | 1 | 100 | 1 | 99 | 1.83e-23 | 88.6 |
| MsG0180005927.01.T01 | AT1G52910 | 44.000 | 100 | 55 | 1 | 1 | 100 | 1 | 99 | 2.29e-23 | 88.6 |
| MsG0180005927.01.T01 | AT4G27435 | 45.833 | 96 | 50 | 1 | 5 | 100 | 6 | 99 | 1.87e-21 | 83.6 |
| MsG0180005927.01.T01 | AT1G13380 | 45.361 | 97 | 53 | 0 | 4 | 100 | 7 | 103 | 1.13e-20 | 82.0 |
| MsG0180005927.01.T01 | AT1G61065 | 39.286 | 84 | 50 | 1 | 17 | 100 | 16 | 98 | 1.21e-14 | 66.2 |
Find 21 sgRNAs with CRISPR-Local
Find 25 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TACAGACACAGCCATGATTT+CGG | 0.392499 | 1:+97648778 | None:intergenic |
| AACAAACAATACAGAGTAAA+GGG | 0.402361 | 1:-97648833 | MsG0180005927.01.T01:five_prime_UTR |
| AATATCTTCAATTCAATGTC+TGG | 0.429834 | 1:+97648883 | None:intergenic |
| CAAATTGAACACCGAAATCA+TGG | 0.450311 | 1:-97648789 | MsG0180005927.01.T01:exon |
| GAACAGTTTGACTAAGAAGC+AGG | 0.481436 | 1:+97648509 | None:intergenic |
| TCATCTTCTTCATTCTCTCA+TGG | 0.481945 | 1:-97648417 | MsG0180005927.01.T01:CDS |
| AGAGATGAAGAGAAATGATA+AGG | 0.482501 | 1:+97648744 | None:intergenic |
| GTTCGGTCATCGTACTCATC+AGG | 0.486965 | 1:+97648589 | None:intergenic |
| AGATGTCTTTGCTGTGGTAA+AGG | 0.489539 | 1:-97648472 | MsG0180005927.01.T01:CDS |
| AAACAAACAATACAGAGTAA+AGG | 0.496962 | 1:-97648834 | MsG0180005927.01.T01:five_prime_UTR |
| TGTGGTAAAGGTCTCATCTC+TGG | 0.498793 | 1:-97648460 | MsG0180005927.01.T01:CDS |
| TGGTGTAAACACAGAAAGTT+CGG | 0.520390 | 1:+97648572 | None:intergenic |
| GAAGAAGATGACAGCGCAAG+TGG | 0.562935 | 1:+97648429 | None:intergenic |
| ACCGACGCTTCCACTGTCTA+CGG | 0.578522 | 1:-97648550 | MsG0180005927.01.T01:CDS |
| CGCTGAACGACGTCGTAGTG+AGG | 0.588172 | 1:-97648699 | MsG0180005927.01.T01:intron |
| AGCAGCAACACCGTAGACAG+TGG | 0.594874 | 1:+97648540 | None:intergenic |
| GTACTCATCAGGAACCACTT+TGG | 0.600621 | 1:+97648600 | None:intergenic |
| ACCGTAGACAGTGGAAGCGT+CGG | 0.606731 | 1:+97648549 | None:intergenic |
| GTCACTAGATGTCTTTGCTG+TGG | 0.617569 | 1:-97648478 | MsG0180005927.01.T01:CDS |
| GTAGACAGTGGAAGCGTCGG+TGG | 0.627400 | 1:+97648552 | None:intergenic |
| ACAGCAAAGACATCTAGTGA+CGG | 0.697347 | 1:+97648480 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTAGTGTTGAAATTAAAGAA+AGG | + | Chr1:97648699-97648718 | None:intergenic | 20.0% |
| ! | AAACAAACAATACAGAGTAA+AGG | - | Chr1:97648528-97648547 | MsG0180005927.01.T01:CDS | 25.0% |
| ! | AACAAACAATACAGAGTAAA+GGG | - | Chr1:97648529-97648548 | MsG0180005927.01.T01:CDS | 25.0% |
| ! | AATATCTTCAATTCAATGTC+TGG | + | Chr1:97648482-97648501 | None:intergenic | 25.0% |
| !! | AATCTGATTAATCTCTTTTC+AGG | - | Chr1:97648739-97648758 | MsG0180005927.01.T01:CDS | 25.0% |
| AGAGATGAAGAGAAATGATA+AGG | + | Chr1:97648621-97648640 | None:intergenic | 30.0% | |
| !! | TTAGCTTTCGTTTTTGCTAT+AGG | - | Chr1:97648640-97648659 | MsG0180005927.01.T01:intron | 30.0% |
| CAAATTGAACACCGAAATCA+TGG | - | Chr1:97648573-97648592 | MsG0180005927.01.T01:CDS | 35.0% | |
| TCATCTTCTTCATTCTCTCA+TGG | - | Chr1:97648945-97648964 | MsG0180005927.01.T01:five_prime_UTR | 35.0% | |
| TGGTGTAAACACAGAAAGTT+CGG | + | Chr1:97648793-97648812 | None:intergenic | 35.0% | |
| ACAGCAAAGACATCTAGTGA+CGG | + | Chr1:97648885-97648904 | None:intergenic | 40.0% | |
| GAACAGTTTGACTAAGAAGC+AGG | + | Chr1:97648856-97648875 | None:intergenic | 40.0% | |
| TACAGACACAGCCATGATTT+CGG | + | Chr1:97648587-97648606 | None:intergenic | 40.0% | |
| ! | AGATGTCTTTGCTGTGGTAA+AGG | - | Chr1:97648890-97648909 | MsG0180005927.01.T01:five_prime_UTR | 40.0% |
| !! | AATCTCTTTTCAGGCCAAAG+TGG | - | Chr1:97648748-97648767 | MsG0180005927.01.T01:CDS | 40.0% |
| GTACTCATCAGGAACCACTT+TGG | + | Chr1:97648765-97648784 | None:intergenic | 45.0% | |
| GTCACTAGATGTCTTTGCTG+TGG | - | Chr1:97648884-97648903 | MsG0180005927.01.T01:five_prime_UTR | 45.0% | |
| TGTGGTAAAGGTCTCATCTC+TGG | - | Chr1:97648902-97648921 | MsG0180005927.01.T01:five_prime_UTR | 45.0% | |
| GAAGAAGATGACAGCGCAAG+TGG | + | Chr1:97648936-97648955 | None:intergenic | 50.0% | |
| GTTCGGTCATCGTACTCATC+AGG | + | Chr1:97648776-97648795 | None:intergenic | 50.0% | |
| ACCGACGCTTCCACTGTCTA+CGG | - | Chr1:97648812-97648831 | MsG0180005927.01.T01:five_prime_UTR | 55.0% | |
| AGCAGCAACACCGTAGACAG+TGG | + | Chr1:97648825-97648844 | None:intergenic | 55.0% | |
| ! | ACCGTAGACAGTGGAAGCGT+CGG | + | Chr1:97648816-97648835 | None:intergenic | 55.0% |
| CGCTGAACGACGTCGTAGTG+AGG | - | Chr1:97648663-97648682 | MsG0180005927.01.T01:intron | 60.0% | |
| !! | GTAGACAGTGGAAGCGTCGG+TGG | + | Chr1:97648813-97648832 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr1 | gene | 97648414 | 97648970 | 97648414 | ID=MsG0180005927.01;Name=MsG0180005927.01 |
| Chr1 | mRNA | 97648414 | 97648970 | 97648414 | ID=MsG0180005927.01.T01;Parent=MsG0180005927.01;Name=MsG0180005927.01.T01;_AED=0.46;_eAED=0.47;_QI=178|1|0.5|1|1|1|2|0|100 |
| Chr1 | exon | 97648414 | 97648623 | 97648414 | ID=MsG0180005927.01.T01:exon:50726;Parent=MsG0180005927.01.T01 |
| Chr1 | exon | 97648700 | 97648970 | 97648700 | ID=MsG0180005927.01.T01:exon:50725;Parent=MsG0180005927.01.T01 |
| Chr1 | five_prime_UTR | 97648793 | 97648970 | 97648793 | ID=MsG0180005927.01.T01:five_prime_utr;Parent=MsG0180005927.01.T01 |
| Chr1 | CDS | 97648700 | 97648792 | 97648700 | ID=MsG0180005927.01.T01:cds;Parent=MsG0180005927.01.T01 |
| Chr1 | CDS | 97648414 | 97648623 | 97648414 | ID=MsG0180005927.01.T01:cds;Parent=MsG0180005927.01.T01 |
| Gene Sequence |
| Protein sequence |
