AlfalfaGEDB | MsG0180006095.01

M. sativa cultivar ZhongmuNo.1 / MsG0180006095.01

KEGG: -
GO: -
PFAM: PF05664

Nr:

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Query id	Subject id	identity %	alignment length	mis matches	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180006095.01.T01	AES62968.1	90.98	255	23	1	255	71	325	3.34E-157	471
MsG0180006095.01.T02	XP_039685998.1	88.421	190	22	123	312	517	706	4.18E-110	348
MsG0180006095.01.T02	XP_039685998.1	94.393	107	6	19	125	335	441	1.64E-56	206

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Swissprot:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180006095.01.T01	Q8RX56	35.714	210	129	2	28	231	260	469	5.31E-29	119
MsG0180006095.01.T02	Q8RX56	36.275	102	63	1	19	120	632	731	1.94E-11	68.6

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Trembl:

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Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180006095.01.T01	G7IE83	90.980	255	23	0	1	255	71	325	1.60e-157	471
MsG0180006095.01.T02	A0A6A4LS52	52.522	337	105	2	19	300	50	386	2.15e-110	339

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TFs/TRs:

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Gene ID	Type	Classification
No data available in table

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Protein Kinases:

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Gene ID	Type	Classification
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Network:

Co-expression Network

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Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080048763.01	MsG0180006095.01	0.815155	1.068799e-51	7.311446e-49
MsG0180003928.01	MsG0180006095.01	0.827424	1.576437e-54	1.521372e-51
MsG0180003937.01	MsG0180006095.01	0.836234	1.054450e-56	1.320936e-53
MsG0180006095.01	MsG0380017946.01	0.818309	2.096033e-52	1.563400e-49
MsG0180006095.01	MsG0480019395.01	0.807545	4.797384e-50	2.679447e-47
MsG0180006095.01	MsG0480022408.01	0.808554	2.925841e-50	1.677983e-47
MsG0180006095.01	MsG0480023460.01	0.810900	9.157873e-51	5.589067e-48
MsG0180006095.01	MsG0680032758.01	0.806384	8.445628e-50	4.577590e-47
MsG0180006095.01	MsG0880042983.01	0.806061	9.875003e-50	5.308743e-47
MsG0180006095.01	MsG0880043921.01	0.806267	8.939546e-50	4.831180e-47

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PPI

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Gene1	Gene2	Type
No data available in table

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180006095.01.T01	MTR_1g114270	90.980	255	23	0	1	255	71	325	4.05e-161	471
MsG0180006095.01.T01	MTR_1g114190	72.277	202	41	1	54	255	807	993	4.80e-87	282
MsG0180006095.01.T01	MTR_3g109630	49.455	275	118	1	2	255	110	384	4.23e-84	271
MsG0180006095.01.T01	MTR_6g043090	43.922	255	143	0	1	255	264	518	5.39e-66	222
MsG0180006095.01.T01	MTR_1g073760	45.923	233	126	0	23	255	151	383	1.06e-64	218
MsG0180006095.01.T01	MTR_1g044480	43.952	248	139	0	15	262	158	405	4.77e-64	216
MsG0180006095.01.T01	MTR_8g040190	34.711	242	145	4	2	231	231	471	2.06e-31	122
MsG0180006095.01.T02	MTR_1g114270	88.421	190	22	0	123	312	645	834	2.46e-113	349
MsG0180006095.01.T02	MTR_1g114270	94.393	107	6	0	19	125	463	569	2.50e-60	207
MsG0180006095.01.T02	MTR_1g114190	55.849	265	59	5	35	260	1116	1361	1.03e-76	255

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Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0180006095.01.T01	AT2G20010	54.887	266	101	3	2	250	74	337	4.21e-90	286
MsG0180006095.01.T01	AT2G25800	56.356	236	101	2	20	255	140	373	8.44e-86	275
MsG0180006095.01.T01	AT2G20010	57.014	221	90	2	33	250	1	219	1.19e-79	256
MsG0180006095.01.T01	AT2G33420	45.276	254	138	1	2	254	153	406	4.28e-70	233
MsG0180006095.01.T01	AT1G04470	47.034	236	122	2	18	252	168	401	2.26e-65	220
MsG0180006095.01.T01	AT5G06970	35.714	210	129	2	28	231	260	469	5.41e-30	119
MsG0180006095.01.T01	AT4G11670	30.415	217	127	6	28	231	230	435	8.59e-19	86.3
MsG0180006095.01.T01	AT4G11670	30.415	217	127	6	28	231	230	435	1.01e-18	85.9
MsG0180006095.01.T01	AT4G11670	30.415	217	127	6	28	231	230	435	1.06e-18	85.9
MsG0180006095.01.T01	AT4G11670	28.333	240	148	6	5	231	202	430	9.77e-17	80.1

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Find 238 sgRNAs with CRISPR-Local

Find 338 sgRNAs with CRISPR-GE

CRISPR-Local

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CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AAAACTGCATGCATGGATAT+GGG	0.490754	1:+100090667	None:intergenic
AAAAGCCACAAATGACTCAG+AGG	0.678635	1:-100090394	MsG0180006095.01.T01:three_prime_UTR
AAAATAGCTTGGAAGACGCA+TGG	0.594063	1:-100092506	MsG0180006095.01.T01:intron
AAAATTCGCAGAAGTGTGTC+GGG	0.522700	1:+100089645	None:intergenic
AAACATCAGAGTGGATAGAC+TGG	0.574255	1:-100091037	MsG0180006095.01.T01:three_prime_UTR
AAACTCCGGCGCATACTCTC+TGG	0.462485	1:-100092319	MsG0180006095.01.T01:CDS
AAAGAGTGTGACATAGATCT+TGG	0.419426	1:+100090117	None:intergenic
AAAGAGTTTGGTAGATCCAA+AGG	0.536803	1:+100092155	None:intergenic
AAATAGGGATCCGGCACCGG+CGG	0.535472	1:-100093426	MsG0180006095.01.T01:five_prime_UTR
AAATCTGAAGTCTTGAACAG+TGG	0.551033	1:+100092454	None:intergenic

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CRISPR-GE

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sgRNA_sequence	Strand	Position	Region	GC_content
AAAGAGTTAAGCCATATGAA+TGG	+	Chr1:100091438-100091457	None:intergenic	30.0%
AAATACAATTGGGTTTCAAC+TGG	-	Chr1:100089755-100089774	MsG0180006095.01.T01:three_prime_UTR	30.0%
AATAATGCTCAAGTTCATGA+AGG	+	Chr1:100092872-100092891	None:intergenic	30.0%
ATCACTAATGATAGCTTACT+AGG	-	Chr1:100090368-100090387	MsG0180006095.01.T01:three_prime_UTR	30.0%
ATGAAACTTCTCCAACATAT+AGG	+	Chr1:100092830-100092849	None:intergenic	30.0%
CAACTTACTAAGGAAATGTA+TGG	-	Chr1:100093185-100093204	MsG0180006095.01.T01:intron	30.0%
CAATATCTATAGGTTTCTCA+AGG	+	Chr1:100090624-100090643	None:intergenic	30.0%
CACAAACAAGCAATAAAAGA+AGG	+	Chr1:100090208-100090227	None:intergenic	30.0%
GCAATTTAGTCAACTTACTA+AGG	-	Chr1:100093175-100093194	MsG0180006095.01.T01:intron	30.0%
GGAATTAGAATTGCTAAGAT+TGG	+	Chr1:100092670-100092689	None:intergenic	30.0%

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Chromosome	Type	Strat	End	Strand	Name
Chr1	gene	100089352	100093584	100089352	ID=MsG0180006095.01;Name=MsG0180006095.01
Chr1	mRNA	100089352	100093584	100089352	ID=MsG0180006095.01.T01;Parent=MsG0180006095.01;Name=MsG0180006095.01.T01;_AED=0.08;_eAED=0.08;_QI=185\|1\|1\|1\|0.25\|0.2\|5\|2031\|262
Chr1	exon	100089352	100090502	100089352	ID=MsG0180006095.01.T01:exon:12690;Parent=MsG0180006095.01.T01
Chr1	exon	100090596	100090785	100090596	ID=MsG0180006095.01.T01:exon:12689;Parent=MsG0180006095.01.T01
Chr1	exon	100091005	100091459	100091005	ID=MsG0180006095.01.T01:exon:12688;Parent=MsG0180006095.01.T01
Chr1	exon	100091660	100092521	100091660	ID=MsG0180006095.01.T01:exon:12687;Parent=MsG0180006095.01.T01
Chr1	exon	100093238	100093584	100093238	ID=MsG0180006095.01.T01:exon:12686;Parent=MsG0180006095.01.T01
Chr1	five_prime_UTR	100093400	100093584	100093400	ID=MsG0180006095.01.T01:five_prime_utr;Parent=MsG0180006095.01.T01
Chr1	CDS	100093238	100093399	100093238	ID=MsG0180006095.01.T01:cds;Parent=MsG0180006095.01.T01
Chr1	CDS	100091895	100092521	100091895	ID=MsG0180006095.01.T01:cds;Parent=MsG0180006095.01.T01
Chr1	three_prime_UTR	100091660	100091894	100091660	ID=MsG0180006095.01.T01:three_prime_utr;Parent=MsG0180006095.01.T01
Chr1	three_prime_UTR	100091005	100091459	100091005	ID=MsG0180006095.01.T01:three_prime_utr;Parent=MsG0180006095.01.T01
Chr1	three_prime_UTR	100090596	100090785	100090596	ID=MsG0180006095.01.T01:three_prime_utr;Parent=MsG0180006095.01.T01
Chr1	three_prime_UTR	100089352	100090502	100089352	ID=MsG0180006095.01.T01:three_prime_utr;Parent=MsG0180006095.01.T01
Chr1	mRNA	100089872	100091877	100089872	ID=MsG0180006095.01.T02;Parent=MsG0180006095.01;Name=MsG0180006095.01.T02;_AED=0.37;_eAED=0.37;_QI=0\|0\|0\|1\|1\|1\|3\|0\|315
Chr1	exon	100091823	100091877	100091823	ID=MsG0180006095.01.T02:exon:12693;Parent=MsG0180006095.01.T02
Chr1	exon	100091005	100091309	100091005	ID=MsG0180006095.01.T02:exon:12692;Parent=MsG0180006095.01.T02
Chr1	exon	100089872	100090459	100089872	ID=MsG0180006095.01.T02:exon:12691;Parent=MsG0180006095.01.T02
Chr1	CDS	100091823	100091877	100091823	ID=MsG0180006095.01.T02:cds;Parent=MsG0180006095.01.T02
Chr1	CDS	100091005	100091309	100091005	ID=MsG0180006095.01.T02:cds;Parent=MsG0180006095.01.T02
Chr1	CDS	100089872	100090459	100089872	ID=MsG0180006095.01.T02:cds;Parent=MsG0180006095.01.T02

Gene Sequence

>MsG0180006095.01.T01

ATGGCGGCTAGTAAGGTGAAGAAAGCTCTTGGACTCAAAACGTCGTCGTTGAAGAATAAGCGTGCAGTGACGACTGGCGAATTGGTGAGAACGCAGATGAGAATCTCTGAGCAGAGTGATACTAGAATTCGAAGAGCACTTCTCAGAATTGCTGCTGCACAGCTTGGAAGACGCATGGAGTTGGTGGTTCTTCCGTTAGAGCTTATACCACTGTTCAAGACTTCAGATTTTTCCAGTCAACAAGAGTATGAGGCTTGGTTGAGAAGAAATTTGAAGGTTCTTGAAGCAGGGCTCCTCTTACATCCGCACATTCCATTAAATAAGGCAGACCCCTCCGCACAGAAACTCCGGCGCATACTCTCTGGAGCCCTTGAGAAACCTATAGATATTGCAAATAGCAGTGAGTCGATGCAAACCCTCCGGAGTATTGTTATCTCTCTTCTTGCAGATCATTTGACGGGTCAGTTCCTGAGACATGTCATTGGGCTGAACGGGTTTCCAATGAACCTTTGGATCTACCAAACTCTTTTAGAAGCTTGTTTTGATACTCATGTTGAAACATGTGTGATTGAAGAGGTTGATGAGGTCTTAGAGCTCATTAAGAAGACCTGGCTTATGCTAGGAATCAATGAAACACTGCATAATATTTGTTTTACATGGGTCTTATTTCGTAGGTATGTCGTCACTCGTGAAATTGAAAATGATCTGCTTTTTGCATCCTGTAATCTGCTGGAGGAAGTTGAGAAAGATACTGAGGCCATGAAGATCCTATTTACTCGAAAGCTTTGA

>MsG0180006095.01.T02

ATGTTGGGTTGGGCAGAGAAAAGGCTCCTTGCTTACCATGATACTTTCCATGACGCTGCCGGTGTTGCGGTTGCTACCCTTCACGTGTGTTATGGAAATGAGTTGAAGAAATATGTTAAAGGCATCAATGATTTGACACCTGATGCTATAGAAGTGCTGATGGCTGCGGACAAGTTGGAGAAAGAGCTGGTGCAGATAGCAGTGGAAGATTCTGCTGACAGCGAAGATGGTGGAAAATCTATTATAATGGAGATCCATCCTTACGAAGCTGAAGCTATAATTGCTAATCTTGTTAAGTCATGGATAAACATCAGAGTGGATAGACTGGCGGAATTGGTTGAAAGAGTTCTGCAACAAGAGGTGTTCAACAAAAGGAAATATCACGGTGTATTCCGGAAAAAGGAAAAGCCACAAATGACTCAGAGGAGGAAAGCCCTTGTTAGTACCACAAACGGAGATAGCTCATTCGATGTACCCCACCTGTGTGTTCGCATCAATACTATGCAGCGTATTCGCATGGAATTAGGGGTTTTGGAAAAGAGGATAGTTGCCAATCTTAGCAATTCTAATTCCACTGGTGAGAATGATATAGCAAATGGGGTGAGCTTCAAGTTTTCCGCAGCTGCTGCGGTGGAAGGTATCCGACAACTATGTGAGTGTATAGCATACAAAGCAGTTTTCCAAGATCTATGTCACACTCTTTGGGACAGCCTATATGTTGGAGAAGTTTCATCCGCAAGGATTGAGCCTTTCCTTCATGAACTTGAGCATTATTTAGAGATCATATCATCAACAGTGCATGATAAAGTTAGAACACGGGTAATTATTGAAGTAATGCGGGCTTCTTTTGATGGTTCCTGTTGGTGGTTGTTGGCGGGAGGTTCATCTCGTGCTTTTTCTTTTGCAAGATTCTTTTGTATTAGAGGAAGATTTCAAGCTTCTGAGTGA

Protein sequence

>MsG0180006095.01.T01

MAASKVKKALGLKTSSLKNKRAVTTGELVRTQMRISEQSDTRIRRALLRIAAAQLGRRMELVVLPLELIPLFKTSDFSSQQEYEAWLRRNLKVLEAGLLLHPHIPLNKADPSAQKLRRILSGALEKPIDIANSSESMQTLRSIVISLLADHLTGQFLRHVIGLNGFPMNLWIYQTLLEACFDTHVETCVIEEVDEVLELIKKTWLMLGINETLHNICFTWVLFRRYVVTREIENDLLFASCNLLEEVEKDTEAMKILFTRKL*

>MsG0180006095.01.T02

MLGWAEKRLLAYHDTFHDAAGVAVATLHVCYGNELKKYVKGINDLTPDAIEVLMAADKLEKELVQIAVEDSADSEDGGKSIIMEIHPYEAEAIIANLVKSWINIRVDRLAELVERVLQQEVFNKRKYHGVFRKKEKPQMTQRRKALVSTTNGDSSFDVPHLCVRINTMQRIRMELGVLEKRIVANLSNSNSTGENDIANGVSFKFSAAAAVEGIRQLCECIAYKAVFQDLCHTLWDSLYVGEVSSARIEPFLHELEHYLEIISSTVHDKVRTRVIIEVMRASFDGSCWWLLAGGSSRAFSFARFFCIRGRFQASE*