AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0180006164.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0180006164.01.T01 MTR_1g115435 62.069 203 57 4 2 184 14 216 3.66e-77 232
MsG0180006164.01.T01 MTR_8g445870 83.516 91 15 0 1 91 13 103 4.63e-51 161
MsG0180006164.01.T01 MTR_1g115440 40.889 225 78 9 1 174 17 237 5.08e-38 132
MsG0180006164.01.T01 MTR_0150s0010 41.850 227 73 10 1 174 16 236 6.91e-37 130
MsG0180006164.01.T01 MTR_8g445860 42.291 227 75 10 1 177 19 239 2.60e-36 128
MsG0180006164.01.T01 MTR_1g115455 34.615 234 82 8 1 177 15 234 3.75e-26 102
MsG0180006164.01.T01 MTR_8g044880 39.200 125 47 4 81 176 154 278 4.31e-17 78.6
MsG0180006164.01.T01 MTR_8g044880 45.122 82 31 3 1 73 14 90 9.76e-13 66.2
MsG0180006164.01.T01 MTR_1g115450 53.521 71 28 2 1 71 13 78 2.10e-14 68.6
MsG0180006164.01.T01 MTR_0150s0030 47.561 82 29 3 1 73 14 90 2.86e-14 69.7
MsG0180006164.01.T01 MTR_8g445910 38.261 115 52 3 81 176 154 268 8.03e-14 69.3
MsG0180006164.01.T01 MTR_8g445910 44.048 84 33 3 1 75 14 92 9.18e-13 66.2
MsG0180006164.01.T01 MTR_4g017830 27.586 203 106 7 1 163 18 219 3.93e-12 65.5
MsG0180006164.01.T01 MTR_8g445920 42.308 78 38 3 1 73 16 91 5.36e-11 58.5
MsG0180006164.01.T01 MTR_8g044890 42.308 78 38 3 1 73 16 91 5.36e-11 58.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 33 sgRNAs with CRISPR-Local

Find 37 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CAAGAAATGGTGGTTGAATT+TGG 0.139334 1:-100876468 MsG0180006164.01.T01:CDS
CATAATTCTGGTTGAGGTTT+TGG 0.163291 1:+100876751 None:intergenic
TCACTATGTTACGAAGAATT+TGG 0.225554 1:-100876221 MsG0180006164.01.T01:CDS
AACATGTTTCACTTATCTTT+TGG 0.271421 1:-100876855 MsG0180006164.01.T01:CDS
AACTTACTGAAATACTTGTT+TGG 0.273357 1:+100876139 None:intergenic
AAATGTAACTTTCATGTTGA+TGG 0.305292 1:-100876574 MsG0180006164.01.T01:CDS
AGTAACGTAATAATTGAAAT+TGG 0.307754 1:+100876525 None:intergenic
GATTGAAGAGGACATAATTC+TGG 0.315260 1:+100876739 None:intergenic
CCACCATTTCTTGCAAGTTA+TGG 0.317364 1:+100876478 None:intergenic
CCTCGATTGTGATCTTCATT+TGG 0.330877 1:+100876708 None:intergenic
ACTTACTGAAATACTTGTTT+GGG 0.340972 1:+100876140 None:intergenic
TCATAGCAACATAACGTGTT+TGG 0.394058 1:+100876604 None:intergenic
CATGTTTAAGAGAGCGTCTA+TGG 0.421041 1:+100876872 None:intergenic
AAGAAATGGTGGTTGAATTT+GGG 0.435875 1:-100876467 MsG0180006164.01.T01:CDS
CGTGTTTGGTCACGGTTCAT+AGG 0.436562 1:+100876618 None:intergenic
AGTCCATAACTTGCAAGAAA+TGG 0.437653 1:-100876481 MsG0180006164.01.T01:CDS
ATCACTGCTATTTGTACTTG+TGG 0.439677 1:-100876108 MsG0180006164.01.T01:CDS
ATGCATTTGTTTGACAGCTT+AGG 0.455380 1:-100876801 MsG0180006164.01.T01:CDS
TGATCTTCATTTGGAATGGT+TGG 0.458438 1:+100876717 None:intergenic
ACATAGCCCAAAACTACAAA+AGG 0.479561 1:+100876192 None:intergenic
AGAGGACATAATTCTGGTTG+AGG 0.493334 1:+100876745 None:intergenic
GAAATACTTGTTTGGGATTG+TGG 0.513857 1:+100876147 None:intergenic
AATAATTGAAATTGGCGAGA+AGG 0.523216 1:+100876533 None:intergenic
ATTGTGATCTTCATTTGGAA+TGG 0.528553 1:+100876713 None:intergenic
ATAATTGAAATTGGCGAGAA+GGG 0.533153 1:+100876534 None:intergenic
TTGGAATGGTTGGATTGAAG+AGG 0.556022 1:+100876727 None:intergenic
CAACATAACGTGTTTGGTCA+CGG 0.566968 1:+100876610 None:intergenic
CCATAACTTGCAAGAAATGG+TGG 0.583549 1:-100876478 MsG0180006164.01.T01:CDS
GGTTGAATTTGGGAAGGATG+AGG 0.589691 1:-100876457 MsG0180006164.01.T01:intron
TGTCTGAATGGATGCACGTA+TGG 0.603734 1:-100876669 MsG0180006164.01.T01:CDS
AATGGTGGTTGAATTTGGGA+AGG 0.648207 1:-100876463 MsG0180006164.01.T01:CDS
CCAAATGAAGATCACAATCG+AGG 0.662124 1:-100876708 MsG0180006164.01.T01:CDS
ATTCAGACACATGTAAAACG+AGG 0.755459 1:+100876683 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AGTAACGTAATAATTGAAAT+TGG + Chr1:100876488-100876507 None:intergenic 20.0%
!!! TATTTTCCTTTTGTAGTTTT+GGG - Chr1:100876812-100876831 MsG0180006164.01.T01:CDS 20.0%
! AAATGTAACTTTCATGTTGA+TGG - Chr1:100876436-100876455 MsG0180006164.01.T01:intron 25.0%
! AACTTACTGAAATACTTGTT+TGG + Chr1:100876874-100876893 None:intergenic 25.0%
! ACTTACTGAAATACTTGTTT+GGG + Chr1:100876873-100876892 None:intergenic 25.0%
!! AACATGTTTCACTTATCTTT+TGG - Chr1:100876155-100876174 MsG0180006164.01.T01:CDS 25.0%
!! GTATTTTCCTTTTGTAGTTT+TGG - Chr1:100876811-100876830 MsG0180006164.01.T01:CDS 25.0%
AAGAAATGGTGGTTGAATTT+GGG - Chr1:100876543-100876562 MsG0180006164.01.T01:CDS 30.0%
AATAATTGAAATTGGCGAGA+AGG + Chr1:100876480-100876499 None:intergenic 30.0%
ATAATTGAAATTGGCGAGAA+GGG + Chr1:100876479-100876498 None:intergenic 30.0%
ATTGTGATCTTCATTTGGAA+TGG + Chr1:100876300-100876319 None:intergenic 30.0%
TCACTATGTTACGAAGAATT+TGG - Chr1:100876789-100876808 MsG0180006164.01.T01:CDS 30.0%
ACATAGCCCAAAACTACAAA+AGG + Chr1:100876821-100876840 None:intergenic 35.0%
ATGCATTTGTTTGACAGCTT+AGG - Chr1:100876209-100876228 MsG0180006164.01.T01:CDS 35.0%
ATTCAGACACATGTAAAACG+AGG + Chr1:100876330-100876349 None:intergenic 35.0%
CAAGAAATGGTGGTTGAATT+TGG - Chr1:100876542-100876561 MsG0180006164.01.T01:CDS 35.0%
GATTGAAGAGGACATAATTC+TGG + Chr1:100876274-100876293 None:intergenic 35.0%
TCATAGCAACATAACGTGTT+TGG + Chr1:100876409-100876428 None:intergenic 35.0%
! AGTCCATAACTTGCAAGAAA+TGG - Chr1:100876529-100876548 MsG0180006164.01.T01:CDS 35.0%
! ATCACTGCTATTTGTACTTG+TGG - Chr1:100876902-100876921 MsG0180006164.01.T01:CDS 35.0%
! GAAATACTTGTTTGGGATTG+TGG + Chr1:100876866-100876885 None:intergenic 35.0%
! TCGTTTTACATGTGTCTGAA+TGG - Chr1:100876329-100876348 MsG0180006164.01.T01:intron 35.0%
! TGATCTTCATTTGGAATGGT+TGG + Chr1:100876296-100876315 None:intergenic 35.0%
!! CATAATTCTGGTTGAGGTTT+TGG + Chr1:100876262-100876281 None:intergenic 35.0%
!!! TTTTGTAGTTTTGGGCTATG+TGG - Chr1:100876820-100876839 MsG0180006164.01.T01:CDS 35.0%
AATGGTGGTTGAATTTGGGA+AGG - Chr1:100876547-100876566 MsG0180006164.01.T01:CDS 40.0%
CAACATAACGTGTTTGGTCA+CGG + Chr1:100876403-100876422 None:intergenic 40.0%
CCAAATGAAGATCACAATCG+AGG - Chr1:100876302-100876321 MsG0180006164.01.T01:intron 40.0%
CCACCATTTCTTGCAAGTTA+TGG + Chr1:100876535-100876554 None:intergenic 40.0%
CCTCGATTGTGATCTTCATT+TGG + Chr1:100876305-100876324 None:intergenic 40.0%
TTGGAATGGTTGGATTGAAG+AGG + Chr1:100876286-100876305 None:intergenic 40.0%
! AGAGGACATAATTCTGGTTG+AGG + Chr1:100876268-100876287 None:intergenic 40.0%
! CCATAACTTGCAAGAAATGG+TGG - Chr1:100876532-100876551 MsG0180006164.01.T01:CDS 40.0%
!! CATGTTTAAGAGAGCGTCTA+TGG + Chr1:100876141-100876160 None:intergenic 40.0%
GGTTGAATTTGGGAAGGATG+AGG - Chr1:100876553-100876572 MsG0180006164.01.T01:CDS 45.0%
TGTCTGAATGGATGCACGTA+TGG - Chr1:100876341-100876360 MsG0180006164.01.T01:intron 45.0%
CGTGTTTGGTCACGGTTCAT+AGG + Chr1:100876395-100876414 None:intergenic 50.0%
Chromosome Type Strat End Strand Name
Chr1 gene 100876086 100876946 100876086 ID=MsG0180006164.01;Name=MsG0180006164.01
Chr1 mRNA 100876086 100876946 100876086 ID=MsG0180006164.01.T01;Parent=MsG0180006164.01;Name=MsG0180006164.01.T01;_AED=0.56;_eAED=0.56;_QI=0|0|0|1|1|1|2|0|232
Chr1 exon 100876458 100876946 100876458 ID=MsG0180006164.01.T01:exon:10031;Parent=MsG0180006164.01.T01
Chr1 exon 100876086 100876295 100876086 ID=MsG0180006164.01.T01:exon:10030;Parent=MsG0180006164.01.T01
Chr1 CDS 100876458 100876946 100876458 ID=MsG0180006164.01.T01:cds;Parent=MsG0180006164.01.T01
Chr1 CDS 100876086 100876295 100876086 ID=MsG0180006164.01.T01:cds;Parent=MsG0180006164.01.T01
Gene Sequence

>MsG0180006164.01.T01

ATGGTTGACACAAAGCAAAACAAAGTTATATTTGCTGAAGCATCAAAAGCTGCCATAGACGCTCTCTTAAACATGTTTCACTTATCTTTTGGTGCTGTTGTAAGACTCATGAGCAACAACGATATGCATTTGTTTGACAGCTTAGGAAATCTGTATCATACTATTACTACTGTCCAAAACCTCAACCAGAATTATGTCCTCTTCAATCCAACCATTCCAAATGAAGATCACAATCGAGGAACCTCGTTTTACATGTGTCTGAATGGATGCACGTATGGTATGACATCGTGTGATCAATATTCACGACCTATGAACCGTGACCAAACACGTTATGTTGCTATGAAAGATCAAAATGTAACTTTCATGTTGATGGATGATTTAGTCATTCATCCCTTCTCGCCAATTTCAATTATTACGTTACTTAACATGTTCGATTTCAAACAAGTCCATAACTTGCAAGAAATGGTGGTTGAATTTGGGAAGGATGAGTCAAAGATTGTTTTGACAAGTGTTTTTATCAAGAACAAGAATAAGAAGAACTTCACTATGTTACGAAGAATTTGGTATTTTCCTTTTGTAGTTTTGGGCTATGTGGTTTATTTCGATTGTCTTCTACCACAATCCCAAACAAGTATTTCAGTAAGTTATCAGTTTATCACTGCTATTTGTACTTGTGGCTTGAAATTAGCATCAAATTAG
Protein sequence

>MsG0180006164.01.T01

MVDTKQNKVIFAEASKAAIDALLNMFHLSFGAVVRLMSNNDMHLFDSLGNLYHTITTVQNLNQNYVLFNPTIPNEDHNRGTSFYMCLNGCTYGMTSCDQYSRPMNRDQTRYVAMKDQNVTFMLMDDLVIHPFSPISIITLLNMFDFKQVHNLQEMVVEFGKDESKIVLTSVFIKNKNKKNFTMLRRIWYFPFVVLGYVVYFDCLLPQSQTSISVSYQFITAICTCGLKLASN*