Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280007331.01.T01 | PNX90420.1 | 69.608 | 204 | 51 | 4 | 2 | 203 | 195 | 389 | 4.45E-85 | 268 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280007331.01.T01 | Q6F2Y7 | 67.488 | 203 | 55 | 4 | 2 | 202 | 196 | 389 | 3.66E-79 | 257 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280007331.01.T01 | A0A2K3MHZ8 | 69.608 | 204 | 51 | 4 | 2 | 203 | 195 | 389 | 2.13e-85 | 268 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180002259.01 | MsG0280007331.01 | 0.800981 | 1.116797e-48 | 5.272583e-46 |
| MsG0180002385.01 | MsG0280007331.01 | 0.812540 | 4.027696e-51 | 2.567885e-48 |
| MsG0180006134.01 | MsG0280007331.01 | 0.827184 | 1.799594e-54 | 1.724575e-51 |
| MsG0180006164.01 | MsG0280007331.01 | 0.843265 | 1.558547e-58 | 2.425261e-55 |
| MsG0280007331.01 | MsG0280009421.01 | 0.801318 | 9.526523e-49 | 4.535845e-46 |
| MsG0280007331.01 | MsG0380013896.01 | 0.809187 | 2.141885e-50 | 1.249221e-47 |
| MsG0280007331.01 | MsG0480021587.01 | 0.802315 | 5.946556e-49 | 2.904004e-46 |
| MsG0280007331.01 | MsG0580025934.01 | 0.805695 | 1.178884e-49 | 6.278695e-47 |
| MsG0280007331.01 | MsG0580026564.01 | 0.813324 | 2.711028e-51 | 1.764958e-48 |
| MsG0280007331.01 | MsG0680031067.01 | 0.827282 | 1.705395e-54 | 1.638988e-51 |
| MsG0280007331.01 | MsG0780036203.01 | 0.811824 | 5.771751e-51 | 3.609322e-48 |
| MsG0280007331.01 | MsG0780036760.01 | 0.836751 | 7.786790e-57 | 9.906272e-54 |
| MsG0280007331.01 | MsG0780038131.01 | 0.805420 | 1.346238e-49 | 7.119094e-47 |
| MsG0280007331.01 | MsG0880044641.01 | 0.808856 | 2.521797e-50 | 1.457733e-47 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280007331.01.T01 | MTR_4g109450 | 70.000 | 200 | 49 | 4 | 2 | 199 | 194 | 384 | 2.95e-82 | 262 |
| MsG0280007331.01.T01 | MTR_4g109450 | 68.966 | 203 | 52 | 4 | 2 | 202 | 194 | 387 | 6.77e-82 | 262 |
| MsG0280007331.01.T01 | MTR_7g028970 | 65.000 | 200 | 59 | 4 | 2 | 199 | 194 | 384 | 2.49e-77 | 249 |
| MsG0280007331.01.T01 | MTR_7g012820 | 56.650 | 203 | 77 | 4 | 2 | 202 | 204 | 397 | 9.17e-65 | 215 |
| MsG0280007331.01.T01 | MTR_7g012820 | 56.650 | 203 | 77 | 4 | 2 | 202 | 275 | 468 | 1.53e-64 | 215 |
| MsG0280007331.01.T01 | MTR_6g013660 | 56.650 | 203 | 77 | 4 | 2 | 202 | 271 | 464 | 1.79e-64 | 215 |
| MsG0280007331.01.T01 | MTR_3g106160 | 54.680 | 203 | 81 | 4 | 2 | 202 | 283 | 476 | 2.44e-62 | 209 |
| MsG0280007331.01.T01 | MTR_8g100040 | 48.276 | 203 | 93 | 5 | 2 | 202 | 291 | 483 | 1.72e-53 | 184 |
| MsG0280007331.01.T01 | MTR_3g498725 | 47.783 | 203 | 94 | 5 | 2 | 202 | 287 | 479 | 2.26e-52 | 181 |
| MsG0280007331.01.T01 | MTR_3g102830 | 47.783 | 203 | 94 | 5 | 2 | 202 | 292 | 484 | 5.62e-52 | 180 |
| MsG0280007331.01.T01 | MTR_3g098310 | 39.320 | 206 | 112 | 5 | 2 | 202 | 330 | 527 | 2.06e-36 | 135 |
| MsG0280007331.01.T01 | MTR_3g098310 | 39.320 | 206 | 112 | 5 | 2 | 202 | 330 | 527 | 2.27e-36 | 135 |
| MsG0280007331.01.T01 | MTR_3g461000 | 36.946 | 203 | 115 | 5 | 2 | 202 | 204 | 395 | 1.03e-32 | 125 |
| MsG0280007331.01.T01 | MTR_6g048860 | 35.135 | 148 | 80 | 6 | 2 | 143 | 168 | 305 | 3.79e-18 | 82.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280007331.01.T01 | AT1G74310 | 66.010 | 203 | 58 | 4 | 2 | 202 | 194 | 387 | 1.85e-79 | 254 |
| MsG0280007331.01.T01 | AT1G74310 | 66.010 | 203 | 58 | 4 | 2 | 202 | 194 | 387 | 6.17e-79 | 254 |
| MsG0280007331.01.T01 | AT4G14670 | 59.500 | 200 | 70 | 3 | 2 | 199 | 159 | 349 | 2.99e-70 | 226 |
| MsG0280007331.01.T01 | AT5G15450 | 55.665 | 203 | 79 | 4 | 2 | 202 | 269 | 462 | 5.87e-64 | 214 |
| MsG0280007331.01.T01 | AT2G25140 | 53.695 | 203 | 83 | 4 | 2 | 202 | 274 | 467 | 3.99e-62 | 208 |
| MsG0280007331.01.T01 | AT3G48870 | 49.020 | 204 | 90 | 5 | 2 | 202 | 279 | 471 | 5.04e-53 | 183 |
| MsG0280007331.01.T01 | AT3G48870 | 49.020 | 204 | 90 | 5 | 2 | 202 | 310 | 502 | 5.46e-53 | 183 |
| MsG0280007331.01.T01 | AT3G48870 | 49.020 | 204 | 90 | 5 | 2 | 202 | 310 | 502 | 5.46e-53 | 183 |
| MsG0280007331.01.T01 | AT3G48870 | 49.020 | 204 | 90 | 5 | 2 | 202 | 310 | 502 | 5.46e-53 | 183 |
| MsG0280007331.01.T01 | AT5G50920 | 48.276 | 203 | 93 | 5 | 2 | 202 | 289 | 481 | 3.80e-52 | 180 |
| MsG0280007331.01.T01 | AT5G51070 | 43.204 | 206 | 104 | 5 | 2 | 202 | 303 | 500 | 1.54e-39 | 144 |
| MsG0280007331.01.T01 | AT3G45450 | 38.095 | 168 | 75 | 6 | 1 | 165 | 168 | 309 | 6.67e-20 | 86.7 |
Find 37 sgRNAs with CRISPR-Local
Find 43 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AATGAAATCCATCTTGTTCT+TGG | 0.244040 | 2:+13773819 | MsG0280007331.01.T01:CDS |
| TGAAGAATACTAATGCTATT+TGG | 0.272506 | 2:-13774020 | None:intergenic |
| AAGAACAATCTTATTCTTGT+AGG | 0.288251 | 2:+13773564 | MsG0280007331.01.T01:CDS |
| AGGATATTTGCTTTAGATAT+CGG | 0.333596 | 2:+13773693 | MsG0280007331.01.T01:CDS |
| AATAGCATTAGTATTCTTCA+TGG | 0.335135 | 2:+13774023 | MsG0280007331.01.T01:CDS |
| CTAATGCTATTTGGAACATT+GGG | 0.390824 | 2:-13774011 | None:intergenic |
| GAAAATGGTAAAACAGATCT+TGG | 0.397235 | 2:+13773654 | MsG0280007331.01.T01:CDS |
| ATCCATCTTGTTCTTGGTGC+TGG | 0.420504 | 2:+13773825 | MsG0280007331.01.T01:CDS |
| CAACAACAATCGAAGATAAC+AGG | 0.423735 | 2:+13773928 | MsG0280007331.01.T01:CDS |
| ACTAATGCTATTTGGAACAT+TGG | 0.437680 | 2:-13774012 | None:intergenic |
| CTTATTCTTGTAGGAGAGCC+AGG | 0.440113 | 2:+13773573 | MsG0280007331.01.T01:CDS |
| CTTGGTGCTGGTAGAATTGA+AGG | 0.466534 | 2:+13773837 | MsG0280007331.01.T01:CDS |
| CTAAGATATCCACCACGATC+AGG | 0.475651 | 2:-13773894 | None:intergenic |
| TATTTGCTAGTGCTAAGAGC+AGG | 0.494954 | 2:+13773721 | MsG0280007331.01.T01:CDS |
| ATTTGAGAGGAGGTTTGTAC+AGG | 0.503602 | 2:+13773974 | MsG0280007331.01.T01:CDS |
| GAGATATACGAGGATCACTA+TGG | 0.507055 | 2:+13774053 | MsG0280007331.01.T01:CDS |
| CTTGTAGGAGAGCCAGGTGT+TGG | 0.507596 | 2:+13773579 | MsG0280007331.01.T01:CDS |
| TATCCACCACGATCAGGCAT+TGG | 0.519946 | 2:-13773888 | None:intergenic |
| GGTGGATATCTTAGATGCAT+TGG | 0.521176 | 2:+13773903 | MsG0280007331.01.T01:CDS |
| GTTGTTGAGGCTCAAAGGAT+AGG | 0.524091 | 2:+13773612 | MsG0280007331.01.T01:CDS |
| CTGTTGTTGTTGAGGCTCAA+AGG | 0.524599 | 2:+13773607 | MsG0280007331.01.T01:CDS |
| TTGTCTAGTCGATACATAGC+TGG | 0.548951 | 2:+13774116 | MsG0280007331.01.T01:CDS |
| AAGATGCAGCATTTGAGAGG+AGG | 0.551112 | 2:+13773964 | MsG0280007331.01.T01:CDS |
| AAGTGGAAGAAGCTCAAGGA+AGG | 0.566087 | 2:+13773781 | MsG0280007331.01.T01:CDS |
| ATTTGCTAGTGCTAAGAGCA+GGG | 0.573543 | 2:+13773722 | MsG0280007331.01.T01:CDS |
| GAGGCTCAAAGGATAGGTAG+AGG | 0.578705 | 2:+13773618 | MsG0280007331.01.T01:CDS |
| TTGGAAATATTGCTAATGCA+AGG | 0.586556 | 2:+13773673 | MsG0280007331.01.T01:CDS |
| GAAGGAAGTTCTGAAAGAAG+TGG | 0.587951 | 2:+13773764 | MsG0280007331.01.T01:CDS |
| CTCAAACCAATGCCTGATCG+TGG | 0.608286 | 2:+13773882 | MsG0280007331.01.T01:CDS |
| TACCAGCACCAAGAACAAGA+TGG | 0.614196 | 2:-13773827 | None:intergenic |
| AAAGAAGTGGAAGAAGCTCA+AGG | 0.619606 | 2:+13773777 | MsG0280007331.01.T01:CDS |
| TTTGCTAGTGCTAAGAGCAG+GGG | 0.637122 | 2:+13773723 | MsG0280007331.01.T01:CDS |
| AAACCAATGCCTGATCGTGG+TGG | 0.639746 | 2:+13773885 | MsG0280007331.01.T01:CDS |
| TGGTAGAATTGAAGGATCGA+CGG | 0.640456 | 2:+13773845 | MsG0280007331.01.T01:CDS |
| AGGCTCAAAGGATAGGTAGA+GGG | 0.645543 | 2:+13773619 | MsG0280007331.01.T01:CDS |
| AGTGGAAGAAGCTCAAGGAA+GGG | 0.658878 | 2:+13773782 | MsG0280007331.01.T01:CDS |
| TGGAATAAAAGAGATATACG+AGG | 0.757207 | 2:+13774043 | MsG0280007331.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | AAGAACAATCTTATTCTTGT+AGG | + | Chr2:13773564-13773583 | MsG0280007331.01.T01:CDS | 25.0% |
| ! | AATAGCATTAGTATTCTTCA+TGG | + | Chr2:13774023-13774042 | MsG0280007331.01.T01:CDS | 25.0% |
| ! | TGAAGAATACTAATGCTATT+TGG | - | Chr2:13774023-13774042 | None:intergenic | 25.0% |
| !! | AGGATATTTGCTTTAGATAT+CGG | + | Chr2:13773693-13773712 | MsG0280007331.01.T01:CDS | 25.0% |
| AATGAAATCCATCTTGTTCT+TGG | + | Chr2:13773819-13773838 | MsG0280007331.01.T01:CDS | 30.0% | |
| GAAAATGGTAAAACAGATCT+TGG | + | Chr2:13773654-13773673 | MsG0280007331.01.T01:CDS | 30.0% | |
| GGGAAAAAAGATCTTGAAAA+TGG | + | Chr2:13773639-13773658 | MsG0280007331.01.T01:CDS | 30.0% | |
| TGGAATAAAAGAGATATACG+AGG | + | Chr2:13774043-13774062 | MsG0280007331.01.T01:CDS | 30.0% | |
| TTGGAAATATTGCTAATGCA+AGG | + | Chr2:13773673-13773692 | MsG0280007331.01.T01:CDS | 30.0% | |
| ! | ACTAATGCTATTTGGAACAT+TGG | - | Chr2:13774015-13774034 | None:intergenic | 30.0% |
| ! | CTAATGCTATTTGGAACATT+GGG | - | Chr2:13774014-13774033 | None:intergenic | 30.0% |
| AAAAAGATGCAGCATTTGAG+AGG | + | Chr2:13773961-13773980 | MsG0280007331.01.T01:CDS | 35.0% | |
| CAACAACAATCGAAGATAAC+AGG | + | Chr2:13773928-13773947 | MsG0280007331.01.T01:CDS | 35.0% | |
| ! | TGGAAAAACTGTTGTTGTTG+AGG | + | Chr2:13773599-13773618 | MsG0280007331.01.T01:CDS | 35.0% |
| !! | AGATTTTGAAGAGAGGTTGA+AGG | + | Chr2:13773746-13773765 | MsG0280007331.01.T01:CDS | 35.0% |
| AAAGAAGTGGAAGAAGCTCA+AGG | + | Chr2:13773777-13773796 | MsG0280007331.01.T01:CDS | 40.0% | |
| ATTTGAGAGGAGGTTTGTAC+AGG | + | Chr2:13773974-13773993 | MsG0280007331.01.T01:CDS | 40.0% | |
| GAGATATACGAGGATCACTA+TGG | + | Chr2:13774053-13774072 | MsG0280007331.01.T01:CDS | 40.0% | |
| GGTGGATATCTTAGATGCAT+TGG | + | Chr2:13773903-13773922 | MsG0280007331.01.T01:CDS | 40.0% | |
| TGGTAGAATTGAAGGATCGA+CGG | + | Chr2:13773845-13773864 | MsG0280007331.01.T01:CDS | 40.0% | |
| TTGTCTAGTCGATACATAGC+TGG | + | Chr2:13774116-13774135 | MsG0280007331.01.T01:CDS | 40.0% | |
| ! | ACAACAGTTTTTCCAACACC+TGG | - | Chr2:13773594-13773613 | None:intergenic | 40.0% |
| ! | ATTTGCTAGTGCTAAGAGCA+GGG | + | Chr2:13773722-13773741 | MsG0280007331.01.T01:CDS | 40.0% |
| ! | TATTTGCTAGTGCTAAGAGC+AGG | + | Chr2:13773721-13773740 | MsG0280007331.01.T01:CDS | 40.0% |
| !! | GAAGGAAGTTCTGAAAGAAG+TGG | + | Chr2:13773764-13773783 | MsG0280007331.01.T01:CDS | 40.0% |
| AAGATGCAGCATTTGAGAGG+AGG | + | Chr2:13773964-13773983 | MsG0280007331.01.T01:CDS | 45.0% | |
| AAGTGGAAGAAGCTCAAGGA+AGG | + | Chr2:13773781-13773800 | MsG0280007331.01.T01:CDS | 45.0% | |
| AGGCTCAAAGGATAGGTAGA+GGG | + | Chr2:13773619-13773638 | MsG0280007331.01.T01:CDS | 45.0% | |
| AGTGGAAGAAGCTCAAGGAA+GGG | + | Chr2:13773782-13773801 | MsG0280007331.01.T01:CDS | 45.0% | |
| CTAAGATATCCACCACGATC+AGG | - | Chr2:13773897-13773916 | None:intergenic | 45.0% | |
| CTTATTCTTGTAGGAGAGCC+AGG | + | Chr2:13773573-13773592 | MsG0280007331.01.T01:CDS | 45.0% | |
| GTTGTTGAGGCTCAAAGGAT+AGG | + | Chr2:13773612-13773631 | MsG0280007331.01.T01:CDS | 45.0% | |
| TACCAGCACCAAGAACAAGA+TGG | - | Chr2:13773830-13773849 | None:intergenic | 45.0% | |
| TTTGCTAGTGCTAAGAGCAG+GGG | + | Chr2:13773723-13773742 | MsG0280007331.01.T01:CDS | 45.0% | |
| ! | ATCCATCTTGTTCTTGGTGC+TGG | + | Chr2:13773825-13773844 | MsG0280007331.01.T01:CDS | 45.0% |
| ! | CTGTTGTTGTTGAGGCTCAA+AGG | + | Chr2:13773607-13773626 | MsG0280007331.01.T01:CDS | 45.0% |
| !! | CTTGGTGCTGGTAGAATTGA+AGG | + | Chr2:13773837-13773856 | MsG0280007331.01.T01:CDS | 45.0% |
| AAACCAATGCCTGATCGTGG+TGG | + | Chr2:13773885-13773904 | MsG0280007331.01.T01:CDS | 50.0% | |
| CTCAAACCAATGCCTGATCG+TGG | + | Chr2:13773882-13773901 | MsG0280007331.01.T01:CDS | 50.0% | |
| GAGGCTCAAAGGATAGGTAG+AGG | + | Chr2:13773618-13773637 | MsG0280007331.01.T01:CDS | 50.0% | |
| TATCCACCACGATCAGGCAT+TGG | - | Chr2:13773891-13773910 | None:intergenic | 50.0% | |
| !! | GCAGGGGAGATTTTGAAGAG+AGG | + | Chr2:13773739-13773758 | MsG0280007331.01.T01:CDS | 50.0% |
| CTTGTAGGAGAGCCAGGTGT+TGG | + | Chr2:13773579-13773598 | MsG0280007331.01.T01:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr2 | gene | 13773543 | 13774208 | 13773543 | ID=MsG0280007331.01;Name=MsG0280007331.01 |
| Chr2 | mRNA | 13773543 | 13774208 | 13773543 | ID=MsG0280007331.01.T01;Parent=MsG0280007331.01;Name=MsG0280007331.01.T01;_AED=0.16;_eAED=0.18;_QI=0|-1|0|1|-1|0|1|0|221 |
| Chr2 | exon | 13773543 | 13774208 | 13773543 | ID=MsG0280007331.01.T01:exon:4137;Parent=MsG0280007331.01.T01 |
| Chr2 | CDS | 13773543 | 13774208 | 13773543 | ID=MsG0280007331.01.T01:cds;Parent=MsG0280007331.01.T01 |
| Gene Sequence |
| Protein sequence |
