AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar ZhongmuNo.1 / MsG0280006332.01

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280006332.01.T01 MTR_2g006180 68.814 295 15 2 145 362 396 690 1.89e-122 369
MsG0280006332.01.T01 MTR_2g006180 90.604 149 8 1 1 143 105 253 1.07e-88 281
MsG0280006332.01.T01 MTR_1g032500 38.961 154 80 3 1 143 91 241 3.03e-21 95.5
MsG0280006332.01.T01 MTR_1g032500 38.961 154 80 3 1 143 91 241 8.97e-21 93.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MsG0280006332.01.T01 AT1G61010 56.271 295 51 2 145 361 398 692 6.43e-96 300
MsG0280006332.01.T01 AT1G61010 80.537 149 23 1 1 143 105 253 5.75e-78 253
MsG0280006332.01.T01 AT1G61010 56.271 295 51 2 145 361 398 692 6.43e-96 300
MsG0280006332.01.T01 AT1G61010 80.537 149 23 1 1 143 105 253 5.75e-78 253
MsG0280006332.01.T01 AT1G61010 56.271 295 51 2 145 361 398 692 6.43e-96 300
MsG0280006332.01.T01 AT1G61010 80.537 149 23 1 1 143 105 253 5.75e-78 253
MsG0280006332.01.T01 AT1G61010 56.271 295 51 2 145 361 398 692 6.43e-96 300
MsG0280006332.01.T01 AT1G61010 80.537 149 23 1 1 143 105 253 5.75e-78 253
MsG0280006332.01.T01 AT1G61010 56.271 295 51 2 145 361 398 692 6.43e-96 300
MsG0280006332.01.T01 AT1G61010 80.537 149 23 1 1 143 105 253 5.75e-78 253
MsG0280006332.01.T01 AT2G01730 33.766 154 88 3 1 143 22 172 1.54e-17 84.3
MsG0280006332.01.T01 AT2G01730 33.766 154 88 3 1 143 91 241 2.41e-17 84.0

Find 67 sgRNAs with CRISPR-Local

Find 232 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
CCTTGGTGCTGCTATGTTTA+TGG 0.100402 2:-538742 MsG0280006332.01.T01:CDS
CAGTTCTCACTCTTTCTTTA+AGG 0.112559 2:+537256 None:intergenic
GCCCTTCTCAATTCACTCTT+TGG 0.225954 2:-537381 MsG0280006332.01.T01:CDS
TGCTCAAACAAGCGCGTTCT+TGG 0.243385 2:-538031 MsG0280006332.01.T01:CDS
TAGTGGTTTGCTAGTTAAAA+AGG 0.258497 2:-537794 MsG0280006332.01.T01:intron
AATGTTAAGGTTGGAGAAAA+TGG 0.270940 2:-537357 MsG0280006332.01.T01:CDS
ATGTTTATGGTTGACATTGC+TGG 0.331772 2:-538729 MsG0280006332.01.T01:CDS
TTCACTCTTTGGAAATGTTA+AGG 0.345990 2:-537370 MsG0280006332.01.T01:CDS
CCACGGGGCTGCTAATGAAA+TGG 0.374527 2:-537977 MsG0280006332.01.T01:CDS
CACACGGCCTCCTTGAGATA+TGG 0.392493 2:+538533 None:intergenic
CACGGGGCTGCTAATGAAAT+GGG 0.399678 2:-537976 MsG0280006332.01.T01:CDS
CCGTCAGTCGATGAAGAATC+TGG 0.409762 2:-537623 MsG0280006332.01.T01:CDS
CTAACGGTTTCACCCACTTC+CGG 0.416006 2:+537814 None:intergenic
AGAGTCAGCATGAGCAGAAA+AGG 0.421643 2:+538053 None:intergenic
TACGGCGCAGAAAGAGGAAT+TGG 0.428244 2:+537204 None:intergenic
CTCTTTGGAAATGTTAAGGT+TGG 0.442169 2:-537366 MsG0280006332.01.T01:CDS
AAAGAGTGAGAACTGCATTC+CGG 0.444582 2:-537248 MsG0280006332.01.T01:CDS
GATTCTATAATGCAGACATC+AGG 0.449087 2:+538627 None:intergenic
AAGCTGATAATTAACATTGA+TGG 0.466063 2:-537333 MsG0280006332.01.T01:CDS
TGATTATGTTAAAGTCAGTA+AGG 0.471668 2:-540615 MsG0280006332.01.T01:CDS
ATGTTAAGGTTGGAGAAAAT+GGG 0.480877 2:-537356 MsG0280006332.01.T01:CDS
TCGCTCCATGGATAAAATTG+AGG 0.496722 2:-540552 MsG0280006332.01.T01:CDS
CGCACGACGAGGGCATAAGC+TGG 0.496749 2:+538504 None:intergenic
TACACTGCAGGCCATGTCCT+TGG 0.497746 2:-538759 MsG0280006332.01.T01:CDS
CTAACATAATTCTTGTCCAC+GGG 0.499152 2:-537993 MsG0280006332.01.T01:CDS
TAAAATACATTTCAACAGAC+TGG 0.506229 2:+537884 None:intergenic
GAAGTGGGTGAAACCGTTAG+TGG 0.507360 2:-537811 MsG0280006332.01.T01:CDS
GGTGTTCGAGTGCTGTATAC+AGG 0.509105 2:-538708 MsG0280006332.01.T01:CDS
TTCCAAAGAGTGAATTGAGA+AGG 0.526712 2:+537379 None:intergenic
AGACATCAGGGGAGAACTGT+GGG 0.528093 2:+538640 None:intergenic
TCGGGGTGTGCCGAGGCTGA+TGG 0.533128 2:+538586 None:intergenic
AAGAACTACGGCGCAGAAAG+AGG 0.539665 2:+537198 None:intergenic
ATAAATCGCTTTCGTCGCAT+AGG 0.541003 2:+540652 None:intergenic
TGCATTATAGAATCCACCTA+TGG 0.545391 2:-538618 MsG0280006332.01.T01:CDS
TTTAACTAGCAAACCACTAA+CGG 0.547846 2:+537798 None:intergenic
AGATTCTGGTGTTACACTGC+AGG 0.548203 2:-538771 MsG0280006332.01.T01:CDS
AATCAGATGCCGTAAAATTG+AGG 0.557395 2:-537439 MsG0280006332.01.T01:intron
ATTCATTCAACCATATCTCA+AGG 0.560477 2:-538543 MsG0280006332.01.T01:CDS
TCCAAAGAGTGAATTGAGAA+GGG 0.562566 2:+537380 None:intergenic
GCAGAACTCAACAAAGAAAG+TGG 0.565463 2:-537303 MsG0280006332.01.T01:CDS
CCGTGGACAAGAATTATGTT+AGG 0.570361 2:+537994 None:intergenic
CGATCATGAACTAGCAACGT+TGG 0.578010 2:+537596 None:intergenic
AAGAGAAAAGAAGACAGAGA+AGG 0.579566 2:-537412 MsG0280006332.01.T01:CDS
GTGTACAACACCATCAGCCT+CGG 0.585238 2:-538596 MsG0280006332.01.T01:CDS
ATTCTATAATGCAGACATCA+GGG 0.587416 2:+538628 None:intergenic
GTAGCTCTTGCGCACGACGA+GGG 0.600503 2:+538494 None:intergenic
CCTAACATAATTCTTGTCCA+CGG 0.607437 2:-537994 MsG0280006332.01.T01:CDS
TCACAGAACTTTGAATACGC+CGG 0.610797 2:+537229 None:intergenic
ACAAGATATTAATCGCTCCA+TGG 0.615255 2:-540564 MsG0280006332.01.T01:CDS
ACATAGCAGCACCAAGGACA+TGG 0.618540 2:+538748 None:intergenic
CCAGATTCTTCATCGACTGA+CGG 0.621492 2:+537623 None:intergenic
TTCTATAATGCAGACATCAG+GGG 0.628451 2:+538629 None:intergenic
CAAACAGATATATGAGAGTG+TGG 0.635327 2:-537648 MsG0280006332.01.T01:CDS
CAGACATCAGGGGAGAACTG+TGG 0.640615 2:+538639 None:intergenic
TGGACAAGAATTATGTTAGG+CGG 0.643977 2:+537997 None:intergenic
ATGGTGTTGTACACCATAGG+TGG 0.651142 2:+538605 None:intergenic
CCATTTCATTAGCAGCCCCG+TGG 0.655342 2:+537977 None:intergenic
GAAGTTGAGAGTGAAAATGA+AGG 0.676670 2:-537279 MsG0280006332.01.T01:CDS
CATAAGCTGGAATCAACACA+CGG 0.678947 2:+538517 None:intergenic
CTGATGGTGTTGTACACCAT+AGG 0.686591 2:+538602 None:intergenic
GGGCTGCTAATGAAATGGGG+AGG 0.691678 2:-537972 MsG0280006332.01.T01:CDS
TACAGGAGACTATTCACGCG+AGG 0.693089 2:-538691 MsG0280006332.01.T01:CDS
CCATAAACATAGCAGCACCA+AGG 0.704532 2:+538742 None:intergenic
ACGGGGCTGCTAATGAAATG+GGG 0.705246 2:-537975 MsG0280006332.01.T01:CDS
GACATCAGGGGAGAACTGTG+GGG 0.718028 2:+538641 None:intergenic
CATTCAACCATATCTCAAGG+AGG 0.733894 2:-538540 MsG0280006332.01.T01:CDS
TAACATAATTCTTGTCCACG+GGG 0.736449 2:-537992 MsG0280006332.01.T01:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AAAAATTACATAATATAAGA+TGG + Chr2:538196-538215 None:intergenic 10.0%
!!! AATAAAGACATAAACTTTTA+TGG - Chr2:538639-538658 MsG0280006332.01.T01:CDS 15.0%
!! AAAATAAACCCTAAATTCAA+CGG + Chr2:538941-538960 None:intergenic 20.0%
!! ACATAGCTATGTATAATATA+AGG - Chr2:538073-538092 MsG0280006332.01.T01:intron 20.0%
!! ACTTTAATTAAGTGTATGTT+TGG - Chr2:537493-537512 MsG0280006332.01.T01:intron 20.0%
!! ATAGAAACGTGTAAAATATA+CGG - Chr2:538132-538151 MsG0280006332.01.T01:intron 20.0%
!! TACACTTAATTAAAGTTTAG+AGG + Chr2:537489-537508 None:intergenic 20.0%
!! TATCAGTTAATTATGTTTCT+GGG - Chr2:537376-537395 MsG0280006332.01.T01:CDS 20.0%
!! TTATCAGTTAATTATGTTTC+TGG - Chr2:537375-537394 MsG0280006332.01.T01:CDS 20.0%
!!! ATTTAGGGTTTATTTTAGTT+TGG - Chr2:538945-538964 MsG0280006332.01.T01:intron 20.0%
!!! ATTTTCAATTTATGGAATAC+AGG - Chr2:537413-537432 MsG0280006332.01.T01:CDS 20.0%
!!! GTATTTTAATTCACAGAAAA+TGG - Chr2:539990-540009 MsG0280006332.01.T01:intron 20.0%
!!! TGGTTTTGATTTTCAATTTA+TGG - Chr2:537405-537424 MsG0280006332.01.T01:CDS 20.0%
!!! TTTTCAATTTATGGAATACA+GGG - Chr2:537414-537433 MsG0280006332.01.T01:CDS 20.0%
! AAAAATTCTTGTGTTATACC+TGG - Chr2:539677-539696 MsG0280006332.01.T01:intron 25.0%
! AAGCTGATAATTAACATTGA+TGG - Chr2:540526-540545 MsG0280006332.01.T01:intron 25.0%
! ATGTTAACAGTAGTAGTAAT+TGG - Chr2:539018-539037 MsG0280006332.01.T01:intron 25.0%
! TAAAATACATTTCAACAGAC+TGG + Chr2:539978-539997 None:intergenic 25.0%
! TAATCTCTGTTAAAATACAC+AGG - Chr2:537952-537971 MsG0280006332.01.T01:CDS 25.0%
! TAGAAACGTGTAAAATATAC+GGG - Chr2:538133-538152 MsG0280006332.01.T01:intron 25.0%
! TGATTATGTTAAAGTCAGTA+AGG - Chr2:537244-537263 MsG0280006332.01.T01:CDS 25.0%
!! AATCATGGTTAATGACTTTT+TGG - Chr2:537898-537917 MsG0280006332.01.T01:CDS 25.0%
!! AATTTCTGCAATGACTTTTT+AGG - Chr2:537760-537779 MsG0280006332.01.T01:intron 25.0%
!! AGGTTTTACGTATCAAATAA+TGG - Chr2:540085-540104 MsG0280006332.01.T01:intron 25.0%
!! ATCATGGTTAATGACTTTTT+GGG - Chr2:537899-537918 MsG0280006332.01.T01:CDS 25.0%
!! ATTTCTGCAATGACTTTTTA+GGG - Chr2:537761-537780 MsG0280006332.01.T01:intron 25.0%
!! GTAATAACTGTATTTCTGTA+AGG + Chr2:538311-538330 None:intergenic 25.0%
!! TAAAATGCATCTTTTCTTTG+TGG - Chr2:537792-537811 MsG0280006332.01.T01:intron 25.0%
!!! AGTTATTGATTTCCATCAAA+CGG - Chr2:539048-539067 MsG0280006332.01.T01:intron 25.0%
!!! ATTATGTTTCTGGGAAATTT+TGG - Chr2:537385-537404 MsG0280006332.01.T01:CDS 25.0%
!!! GATTTTTAGTGAATAGATTC+TGG - Chr2:537535-537554 MsG0280006332.01.T01:intron 25.0%
AAAGTCATTGCAGAAATTGT+AGG + Chr2:537758-537777 None:intergenic 30.0%
AAATTGTAGGGGGTAAAATT+GGG + Chr2:537745-537764 None:intergenic 30.0%
AAGTCATTGCAGAAATTGTA+GGG + Chr2:537757-537776 None:intergenic 30.0%
AATCCTGTCATTCATTGAAA+GGG + Chr2:539499-539518 None:intergenic 30.0%
AATGTTAAGGTTGGAGAAAA+TGG - Chr2:540502-540521 MsG0280006332.01.T01:intron 30.0%
AGAAAATGGCAAAAACCATT+GGG - Chr2:540004-540023 MsG0280006332.01.T01:intron 30.0%
AGCTATGTATAATATAAGGC+AGG - Chr2:538077-538096 MsG0280006332.01.T01:intron 30.0%
AGTTATTACTGATATTGCTG+CGG - Chr2:538322-538341 MsG0280006332.01.T01:intron 30.0%
ATGTTAAGGTTGGAGAAAAT+GGG - Chr2:540503-540522 MsG0280006332.01.T01:intron 30.0%
ATTCATTCAACCATATCTCA+AGG - Chr2:539316-539335 MsG0280006332.01.T01:intron 30.0%
ATTCTATAATGCAGACATCA+GGG + Chr2:539234-539253 None:intergenic 30.0%
ATTTGCCTTTAAGCACATAT+CGG - Chr2:539539-539558 MsG0280006332.01.T01:intron 30.0%
CATAATAGATGGGTATATTC+TGG + Chr2:539431-539450 None:intergenic 30.0%
GACATAAGATAAATTGTTGC+AGG + Chr2:538699-538718 None:intergenic 30.0%
GCCAAAGTTAATTCTACTTA+AGG - Chr2:537684-537703 MsG0280006332.01.T01:intron 30.0%
GCCTTAAGTAGAATTAACTT+TGG + Chr2:537688-537707 None:intergenic 30.0%
GTTATCATAACGAGTAATCA+TGG - Chr2:537883-537902 MsG0280006332.01.T01:CDS 30.0%
TATTCACATTTGGCAATTTG+AGG + Chr2:538469-538488 None:intergenic 30.0%
TTCAATTGCCGTTGAATTTA+GGG - Chr2:538930-538949 MsG0280006332.01.T01:intron 30.0%
TTGATGGAAATCAATAACTG+CGG + Chr2:539047-539066 None:intergenic 30.0%
TTGTTGAACACTTGATATTG+TGG + Chr2:538875-538894 None:intergenic 30.0%
TTTAACTAGCAAACCACTAA+CGG + Chr2:540064-540083 None:intergenic 30.0%
! ACATATACTTGTAGCGTTTT+TGG - Chr2:538537-538556 MsG0280006332.01.T01:CDS 30.0%
! ATTGACATTTTCAAAGACGT+AGG - Chr2:539573-539592 MsG0280006332.01.T01:intron 30.0%
! TTCACTCTTTGGAAATGTTA+AGG - Chr2:540489-540508 MsG0280006332.01.T01:intron 30.0%
!! TAAAACTGGTTTGACATGTT+TGG + Chr2:537710-537729 None:intergenic 30.0%
!! TAGTGGTTTGCTAGTTAAAA+AGG - Chr2:540065-540084 MsG0280006332.01.T01:intron 30.0%
!!! GTCAAACATTTTTTTGCAAG+AGG + Chr2:539459-539478 None:intergenic 30.0%
AAGAGAAAAGAAGACAGAGA+AGG - Chr2:540447-540466 MsG0280006332.01.T01:intron 35.0%
AAGGAAGTTACTCTGATGAA+TGG - Chr2:539745-539764 MsG0280006332.01.T01:intron 35.0%
AAGTGAATGGTATCAGATTC+TGG - Chr2:539074-539093 MsG0280006332.01.T01:intron 35.0%
AATCAGATGCCGTAAAATTG+AGG - Chr2:540420-540439 MsG0280006332.01.T01:intron 35.0%
ACAAGATATTAATCGCTCCA+TGG - Chr2:537295-537314 MsG0280006332.01.T01:CDS 35.0%
ACTGCATATTTGCATATCAC+TGG + Chr2:538427-538446 None:intergenic 35.0%
ACTGTGAAATGTTTATGCTG+TGG - Chr2:538379-538398 MsG0280006332.01.T01:intron 35.0%
AGAATTACCATCCCTTATTC+AGG - Chr2:540161-540180 MsG0280006332.01.T01:intron 35.0%
AGTCATTGCAGAAATTGTAG+GGG + Chr2:537756-537775 None:intergenic 35.0%
ATACCTGGATATGTTGTTGA+GGG - Chr2:539692-539711 MsG0280006332.01.T01:intron 35.0%
ATAGCTATGTGATTCGTAGT+AGG + Chr2:538063-538082 None:intergenic 35.0%
ATAGTCGTTCACTGTAATCA+TGG - Chr2:537824-537843 MsG0280006332.01.T01:CDS 35.0%
ATATTTGCATATCACTGGGT+GGG + Chr2:538422-538441 None:intergenic 35.0%
ATCTGATACCATGTCACTAA+TGG + Chr2:540345-540364 None:intergenic 35.0%
ATGTTTATGGTTGACATTGC+TGG - Chr2:539130-539149 MsG0280006332.01.T01:intron 35.0%
ATTCATCAGAGTAACTTCCT+TGG + Chr2:539746-539765 None:intergenic 35.0%
CAAACAGATATATGAGAGTG+TGG - Chr2:540211-540230 MsG0280006332.01.T01:intron 35.0%
CAGAAAATGGCAAAAACCAT+TGG - Chr2:540003-540022 MsG0280006332.01.T01:intron 35.0%
CAGTTCTCACTCTTTCTTTA+AGG + Chr2:540606-540625 None:intergenic 35.0%
CCTAACATAATTCTTGTCCA+CGG - Chr2:539865-539884 MsG0280006332.01.T01:intron 35.0%
CGATGCACTTTATTCACATT+TGG + Chr2:538479-538498 None:intergenic 35.0%
CTAACATAATTCTTGTCCAC+GGG - Chr2:539866-539885 MsG0280006332.01.T01:intron 35.0%
CTGCATATTTGCATATCACT+GGG + Chr2:538426-538445 None:intergenic 35.0%
CTTCAATTGCCGTTGAATTT+AGG - Chr2:538929-538948 MsG0280006332.01.T01:intron 35.0%
GAAAACTGTGTATGATGCAT+TGG + Chr2:538910-538929 None:intergenic 35.0%
GAAATTGTAGGGGGTAAAAT+TGG + Chr2:537746-537765 None:intergenic 35.0%
GAAGTTGAGAGTGAAAATGA+AGG - Chr2:540580-540599 MsG0280006332.01.T01:CDS 35.0%
GAATCCTGTCATTCATTGAA+AGG + Chr2:539500-539519 None:intergenic 35.0%
GATAAATTGTTGCAGGTTGT+AGG + Chr2:538692-538711 None:intergenic 35.0%
GATATGTGCTTAAAGGCAAA+TGG + Chr2:539540-539559 None:intergenic 35.0%
GATTCTATAATGCAGACATC+AGG + Chr2:539235-539254 None:intergenic 35.0%
GGTGTCTAGATGTATAAAAC+TGG + Chr2:537724-537743 None:intergenic 35.0%
GTGTTAACAGTAGTACTAGT+TGG - Chr2:538025-538044 MsG0280006332.01.T01:CDS 35.0%
GTTGATCCTTGATGAGTATT+GGG - Chr2:539389-539408 MsG0280006332.01.T01:intron 35.0%
TAACATAATTCTTGTCCACG+GGG - Chr2:539867-539886 MsG0280006332.01.T01:intron 35.0%
TATACCTGGATATGTTGTTG+AGG - Chr2:539691-539710 MsG0280006332.01.T01:intron 35.0%
TCCAAAGAGTGAATTGAGAA+GGG + Chr2:540482-540501 None:intergenic 35.0%
TCTACTCTGTATGACACAAA+AGG + Chr2:540189-540208 None:intergenic 35.0%
TCTGATACCATGTCACTAAT+GGG + Chr2:540344-540363 None:intergenic 35.0%
TGATTTATCCGTTGCAATTC+TGG + Chr2:538737-538756 None:intergenic 35.0%
TGCATTATAGAATCCACCTA+TGG - Chr2:539241-539260 MsG0280006332.01.T01:intron 35.0%
TGGACAAGAATTATGTTAGG+CGG + Chr2:539865-539884 None:intergenic 35.0%
TGTTGATCCTTGATGAGTAT+TGG - Chr2:539388-539407 MsG0280006332.01.T01:intron 35.0%
TTCCAAAGAGTGAATTGAGA+AGG + Chr2:540483-540502 None:intergenic 35.0%
TTCTATAATGCAGACATCAG+GGG + Chr2:539233-539252 None:intergenic 35.0%
! AAGAGGAGAAGCATAATAGA+TGG + Chr2:539442-539461 None:intergenic 35.0%
! AGAGGAGAAGCATAATAGAT+GGG + Chr2:539441-539460 None:intergenic 35.0%
! CTCTTCTCTCCTCAATTTTA+CGG + Chr2:540432-540451 None:intergenic 35.0%
! CTCTTTGGAAATGTTAAGGT+TGG - Chr2:540493-540512 MsG0280006332.01.T01:intron 35.0%
! GCTAACCTCAATTTTATCCA+TGG + Chr2:537315-537334 None:intergenic 35.0%
! TACCTTTTCCAGAATTGCAA+CGG - Chr2:538726-538745 MsG0280006332.01.T01:CDS 35.0%
! TCGCGACAATTTTTGATATG+TGG - Chr2:539642-539661 MsG0280006332.01.T01:intron 35.0%
! TCTGATTCAGCCACTATTTT+TGG + Chr2:540407-540426 None:intergenic 35.0%
!! ACATCAGTAAAGCGTTTTCT+CGG + Chr2:539295-539314 None:intergenic 35.0%
!! AGCTTCAAGTTGATCAATTC+TGG - Chr2:537660-537679 MsG0280006332.01.T01:intron 35.0%
!! CTGATTCAGCCACTATTTTT+GGG + Chr2:540406-540425 None:intergenic 35.0%
!! TGTGTATGATGCATTGGTTT+GGG + Chr2:538904-538923 None:intergenic 35.0%
AAACCATCCTCAATGAACCA+AGG - Chr2:539726-539745 MsG0280006332.01.T01:intron 40.0%
AAAGAGTGAGAACTGCATTC+CGG - Chr2:540611-540630 MsG0280006332.01.T01:CDS 40.0%
ACACCCTTTCAATGAATGAC+AGG - Chr2:539493-539512 MsG0280006332.01.T01:intron 40.0%
ATAAATCGCTTTCGTCGCAT+AGG + Chr2:537210-537229 None:intergenic 40.0%
CAAAGCCGATATGTGCTTAA+AGG + Chr2:539547-539566 None:intergenic 40.0%
CAGGTTGTAGGACTTTATAC+AGG + Chr2:538680-538699 None:intergenic 40.0%
CATAAGCTGGAATCAACACA+CGG + Chr2:539345-539364 None:intergenic 40.0%
CATATTTGCATATCACTGGG+TGG + Chr2:538423-538442 None:intergenic 40.0%
CATCAAACGGTTGAAGTGAA+TGG - Chr2:539061-539080 MsG0280006332.01.T01:intron 40.0%
CATTCAACCATATCTCAAGG+AGG - Chr2:539319-539338 MsG0280006332.01.T01:intron 40.0%
CATTCACTTCAACCGTTTGA+TGG + Chr2:539063-539082 None:intergenic 40.0%
CCGTGGACAAGAATTATGTT+AGG + Chr2:539868-539887 None:intergenic 40.0%
CTGATACCATGTCACTAATG+GGG + Chr2:540343-540362 None:intergenic 40.0%
CTTGATATTGTGGAGTTGTG+TGG + Chr2:538865-538884 None:intergenic 40.0%
GAAGTATGTCTCCTTGCATT+GGG - Chr2:540310-540329 MsG0280006332.01.T01:intron 40.0%
GATTTGCCCAATACTCATCA+AGG + Chr2:539398-539417 None:intergenic 40.0%
GCAGAACTCAACAAAGAAAG+TGG - Chr2:540556-540575 MsG0280006332.01.T01:CDS 40.0%
GTAACTTCCTTGGTTCATTG+AGG + Chr2:539736-539755 None:intergenic 40.0%
GTCATTGCAGAAATTGTAGG+GGG + Chr2:537755-537774 None:intergenic 40.0%
TACCTGGATATGTTGTTGAG+GGG - Chr2:539693-539712 MsG0280006332.01.T01:intron 40.0%
TATTTGCATATCACTGGGTG+GGG + Chr2:538421-538440 None:intergenic 40.0%
TCACAGAACTTTGAATACGC+CGG + Chr2:540633-540652 None:intergenic 40.0%
TCGCTCCATGGATAAAATTG+AGG - Chr2:537307-537326 MsG0280006332.01.T01:CDS 40.0%
TGTCTTCTCACAGCTATCAA+CGG - Chr2:540124-540143 MsG0280006332.01.T01:intron 40.0%
! AGAAGTATGTCTCCTTGCAT+TGG - Chr2:540309-540328 MsG0280006332.01.T01:intron 40.0%
! ATCCGTTGCAATTCTGGAAA+AGG + Chr2:538731-538750 None:intergenic 40.0%
! GTCATTCATTGAAAGGGTGT+AGG + Chr2:539493-539512 None:intergenic 40.0%
! GTGTGGTTTTCAGACAATGA+AGG + Chr2:538848-538867 None:intergenic 40.0%
! TTGATAGCTGTGAGAAGACA+TGG + Chr2:540124-540143 None:intergenic 40.0%
!! ATCAGTAAAGCGTTTTCTCG+GGG + Chr2:539293-539312 None:intergenic 40.0%
!! CATCAGTAAAGCGTTTTCTC+GGG + Chr2:539294-539313 None:intergenic 40.0%
!! CTGTGTATGATGCATTGGTT+TGG + Chr2:538905-538924 None:intergenic 40.0%
!! TGAGTATTGGGCAAATCATC+CGG - Chr2:539401-539420 MsG0280006332.01.T01:intron 40.0%
!!! ATTCAGCCACTATTTTTGGG+AGG + Chr2:540403-540422 None:intergenic 40.0%
!!! GTTTTTTGGAGTGAGGATCT+TGG + Chr2:539953-539972 None:intergenic 40.0%
!!! TCAACAGACTGGCAGTTTTT+TGG + Chr2:539967-539986 None:intergenic 40.0%
ACATGAATGCACTCGCTTGA+TGG + Chr2:538819-538838 None:intergenic 45.0%
ACGGGCATGAGTTATGAGAT+TGG - Chr2:538151-538170 MsG0280006332.01.T01:intron 45.0%
AGAGTCAGCATGAGCAGAAA+AGG + Chr2:539809-539828 None:intergenic 45.0%
ATATGTTGTTGAGGGGACAC+TGG - Chr2:539700-539719 MsG0280006332.01.T01:intron 45.0%
ATTTGCATATCACTGGGTGG+GGG + Chr2:538420-538439 None:intergenic 45.0%
CCATAAACATAGCAGCACCA+AGG + Chr2:539120-539139 None:intergenic 45.0%
CGATCATGAACTAGCAACGT+TGG + Chr2:540266-540285 None:intergenic 45.0%
CTTCCTTGGTTCATTGAGGA+TGG + Chr2:539732-539751 None:intergenic 45.0%
GAAGCTGGCTGAAAAAACAC+CGG - Chr2:540026-540045 MsG0280006332.01.T01:intron 45.0%
GAGAAGACATGGAGATCATC+TGG + Chr2:540113-540132 None:intergenic 45.0%
GCCCTTCTCAATTCACTCTT+TGG - Chr2:540478-540497 MsG0280006332.01.T01:intron 45.0%
GCTGAAAAAACACCGGAAGT+GGG - Chr2:540033-540052 MsG0280006332.01.T01:intron 45.0%
TACCTAACCAGAGTGAGTTG+CGG - Chr2:538242-538261 MsG0280006332.01.T01:intron 45.0%
! CCAGATTCTTCATCGACTGA+CGG + Chr2:540239-540258 None:intergenic 45.0%
!! ACACAAAAGGCGCCTGAATA+AGG + Chr2:540176-540195 None:intergenic 45.0%
!! AGATTCTGGTGTTACACTGC+AGG - Chr2:539088-539107 MsG0280006332.01.T01:intron 45.0%
!! ATGGGTATATTCTGGAGCTC+CGG + Chr2:539423-539442 None:intergenic 45.0%
!! ATGGTGTTGTACACCATAGG+TGG + Chr2:539257-539276 None:intergenic 45.0%
!! CACAAAAGGCGCCTGAATAA+GGG + Chr2:540175-540194 None:intergenic 45.0%
!! CCTTGGTGCTGCTATGTTTA+TGG - Chr2:539117-539136 MsG0280006332.01.T01:intron 45.0%
!! CTGATGGTGTTGTACACCAT+AGG + Chr2:539260-539279 None:intergenic 45.0%
!! TTTTTCAGCCAGCTTCCCAA+TGG + Chr2:540022-540041 None:intergenic 45.0%
!!! ACTGGCAGTTTTTTGGAGTG+AGG + Chr2:539960-539979 None:intergenic 45.0%
!!! CCACTATTTTTGGGAGGTCA+CGG + Chr2:540397-540416 None:intergenic 45.0%
!!! TACTTGTAGCGTTTTTGGCG+TGG - Chr2:538542-538561 MsG0280006332.01.T01:CDS 45.0%
ACATAGCAGCACCAAGGACA+TGG + Chr2:539114-539133 None:intergenic 50.0%
ACGGGGCTGCTAATGAAATG+GGG - Chr2:539884-539903 MsG0280006332.01.T01:intron 50.0%
AGACATCAGGGGAGAACTGT+GGG + Chr2:539222-539241 None:intergenic 50.0%
ATCATCACCGCAACTCACTC+TGG + Chr2:538252-538271 None:intergenic 50.0%
CAAAGACGTAGGACCATCTG+TGG - Chr2:539584-539603 MsG0280006332.01.T01:intron 50.0%
CACGGGGCTGCTAATGAAAT+GGG - Chr2:539883-539902 MsG0280006332.01.T01:intron 50.0%
CCGTCAGTCGATGAAGAATC+TGG - Chr2:540236-540255 MsG0280006332.01.T01:intron 50.0%
CCGTGACCTCCCAAAAATAG+TGG - Chr2:540394-540413 MsG0280006332.01.T01:intron 50.0%
CTAACGGTTTCACCCACTTC+CGG + Chr2:540048-540067 None:intergenic 50.0%
CTCTGCAACTCTCAACATGC+AGG - Chr2:539771-539790 MsG0280006332.01.T01:intron 50.0%
GAAGTGGGTGAAACCGTTAG+TGG - Chr2:540048-540067 MsG0280006332.01.T01:intron 50.0%
GGCAAAAACCATTGGGAAGC+TGG - Chr2:540011-540030 MsG0280006332.01.T01:intron 50.0%
GGCTGAAAAAACACCGGAAG+TGG - Chr2:540032-540051 MsG0280006332.01.T01:intron 50.0%
GTCAGACCCCATTAGTGACA+TGG - Chr2:540334-540353 MsG0280006332.01.T01:intron 50.0%
GTCCCCTCAACAACATATCC+AGG + Chr2:539698-539717 None:intergenic 50.0%
GTGTACAACACCATCAGCCT+CGG - Chr2:539263-539282 MsG0280006332.01.T01:intron 50.0%
TACAGGAGACTATTCACGCG+AGG - Chr2:539168-539187 MsG0280006332.01.T01:intron 50.0%
TACGGCGCAGAAAGAGGAAT+TGG + Chr2:540658-540677 None:intergenic 50.0%
TGCTCAAACAAGCGCGTTCT+TGG - Chr2:539828-539847 MsG0280006332.01.T01:intron 50.0%
TTTGCATATCACTGGGTGGG+GGG + Chr2:538419-538438 None:intergenic 50.0%
! GGTGTTCGAGTGCTGTATAC+AGG - Chr2:539151-539170 MsG0280006332.01.T01:intron 50.0%
!! AAAGGCGCCTGAATAAGGGA+TGG + Chr2:540171-540190 None:intergenic 50.0%
!!! AGCGTTTTTGGCGTGGAGAT+AGG - Chr2:538549-538568 MsG0280006332.01.T01:CDS 50.0%
CACACGGCCTCCTTGAGATA+TGG + Chr2:539329-539348 None:intergenic 55.0%
CACCGCAACTCACTCTGGTT+AGG + Chr2:538247-538266 None:intergenic 55.0%
CAGACATCAGGGGAGAACTG+TGG + Chr2:539223-539242 None:intergenic 55.0%
CCACGGGGCTGCTAATGAAA+TGG - Chr2:539882-539901 MsG0280006332.01.T01:intron 55.0%
CCATTTCATTAGCAGCCCCG+TGG + Chr2:539885-539904 None:intergenic 55.0%
CGTAGGACCATCTGTGGTGA+TGG - Chr2:539590-539609 MsG0280006332.01.T01:intron 55.0%
GACATCAGGGGAGAACTGTG+GGG + Chr2:539221-539240 None:intergenic 55.0%
GGGCTGCTAATGAAATGGGG+AGG - Chr2:539887-539906 MsG0280006332.01.T01:intron 55.0%
TACACTGCAGGCCATGTCCT+TGG - Chr2:539100-539119 MsG0280006332.01.T01:intron 55.0%
TTGCATATCACTGGGTGGGG+GGG + Chr2:538418-538437 None:intergenic 55.0%
!! AACCCACTCTGAAGTCCACC+AGG + Chr2:539621-539640 None:intergenic 55.0%
!! ACCCACTCTGAAGTCCACCA+GGG + Chr2:539620-539639 None:intergenic 55.0%
AGTAGCTCTTGCGCACGACG+AGG + Chr2:539369-539388 None:intergenic 60.0%
GTAGCTCTTGCGCACGACGA+GGG + Chr2:539368-539387 None:intergenic 60.0%
TGCATATCACTGGGTGGGGG+GGG + Chr2:538417-538436 None:intergenic 60.0%
!! AGCCCTGGTGGACTTCAGAG+TGG - Chr2:539615-539634 MsG0280006332.01.T01:intron 60.0%
!! GCCCTGGTGGACTTCAGAGT+GGG - Chr2:539616-539635 MsG0280006332.01.T01:intron 60.0%
ATATCACTGGGTGGGGGGGG+GGG + Chr2:538414-538433 None:intergenic 65.0%
CATATCACTGGGTGGGGGGG+GGG + Chr2:538415-538434 None:intergenic 65.0%
GCATATCACTGGGTGGGGGG+GGG + Chr2:538416-538435 None:intergenic 65.0%
GGGCTCGCCATCACCACAGA+TGG + Chr2:539600-539619 None:intergenic 65.0%
GGGGTCTGACGCCCAATGCA+AGG + Chr2:540324-540343 None:intergenic 65.0%
! CGCACGACGAGGGCATAAGC+TGG + Chr2:539358-539377 None:intergenic 65.0%
! CGTTTTCTCGGGGTGTGCCG+AGG + Chr2:539283-539302 None:intergenic 65.0%
!! TCTGTGGTGATGGCGAGCCC+TGG - Chr2:539600-539619 MsG0280006332.01.T01:intron 65.0%
TATCACTGGGTGGGGGGGGG+GGG + Chr2:538413-538432 None:intergenic 70.0%
TCGGGGTGTGCCGAGGCTGA+TGG + Chr2:539276-539295 None:intergenic 70.0%
!! GTGGTGATGGCGAGCCCTGG+TGG - Chr2:539603-539622 MsG0280006332.01.T01:intron 70.0%
ATCACTGGGTGGGGGGGGGG+GGG + Chr2:538412-538431 None:intergenic 75.0%
! TCACTGGGTGGGGGGGGGGG+GGG + Chr2:538411-538430 None:intergenic 80.0%
! ACTGGGTGGGGGGGGGGGGG+GGG + Chr2:538409-538428 None:intergenic 85.0%
! CACTGGGTGGGGGGGGGGGG+GGG + Chr2:538410-538429 None:intergenic 85.0%
Chromosome Type Strat End Strand Name
Chr2 gene 537203 540678 537203 ID=MsG0280006332.01;Name=MsG0280006332.01
Chr2 mRNA 537203 540678 537203 ID=MsG0280006332.01.T01;Parent=MsG0280006332.01;Name=MsG0280006332.01.T01;_AED=0.49;_eAED=0.50;_QI=0|0|0|1|0.75|0.6|5|0|362
Chr2 exon 540530 540678 540530 ID=MsG0280006332.01.T01:exon:752;Parent=MsG0280006332.01.T01
Chr2 exon 538509 538791 538509 ID=MsG0280006332.01.T01:exon:751;Parent=MsG0280006332.01.T01
Chr2 exon 537795 538088 537795 ID=MsG0280006332.01.T01:exon:750;Parent=MsG0280006332.01.T01
Chr2 exon 537569 537678 537569 ID=MsG0280006332.01.T01:exon:749;Parent=MsG0280006332.01.T01
Chr2 exon 537203 537455 537203 ID=MsG0280006332.01.T01:exon:748;Parent=MsG0280006332.01.T01
Chr2 CDS 540530 540678 540530 ID=MsG0280006332.01.T01:cds;Parent=MsG0280006332.01.T01
Chr2 CDS 538509 538791 538509 ID=MsG0280006332.01.T01:cds;Parent=MsG0280006332.01.T01
Chr2 CDS 537795 538088 537795 ID=MsG0280006332.01.T01:cds;Parent=MsG0280006332.01.T01
Chr2 CDS 537569 537678 537569 ID=MsG0280006332.01.T01:cds;Parent=MsG0280006332.01.T01
Chr2 CDS 537203 537455 537203 ID=MsG0280006332.01.T01:cds;Parent=MsG0280006332.01.T01
Gene Sequence

>MsG0280006332.01.T01

ATGACCTATGCGACGAAAGCGATTTATAAGCTTCTTTTGTCTGATTATGTTAAAGTCAGTAAGGTTTCTGTTGATGATATGCTTTATGATGAACAAGATATTAATCGCTCCATGGATAAAATTGAGGTTAGCTTCTTTATTGCGTTAATATTCTGGTGTTACACTGCAGGCCATGTCCTTGGTGCTGCTATGTTTATGGTTGACATTGCTGGTGTTCGAGTGCTGTATACAGGAGACTATTCACGCGAGGAAGACAGACATCTTCGAGCTGCAGAGACCCCACAGTTCTCCCCTGATGTCTGCATTATAGAATCCACCTATGGTGTACAACACCATCAGCCTCGGCACACCCCGAGAAAACGCTTTACTGATGTTATTCATTCAACCATATCTCAAGGAGGCCGTGTGTTGATTCCAGCTTATGCCCTCGTCGTGCACTACATTTCCTTTTCTGCTCATGCTGACTCTGCTCAAACAAGCGCGTTCTTGGAAGAGCTCAATCCGCCTAACATAATTCTTGTCCACGGGGCTGCTAATGAAATGGGGAGGCTCAAGCAGAAGCTCATGACTCAGTTTGCTGATCGTAACACCAAGATCCTCACTCCAAAAAACTGCCAGTCTGTTGAAATGTATTTTAATTCACAGAAAATGGCAAAAACCATTGGGAAGCTGGCTGAAAAAACACCGGAAGTGGGTGAAACCGTTAGTGGTTTGCTAGTTAAAAAGAGTAGACTCAAACAGATATATGAGAGTGTGGAGCCGTCAGTCGATGAAGAATCTGGAGTTCCAACGTTGCTAGTTCATGATCGTGTAACAGTTAAGCATGAGTCTGAGAAATGCCGTAAAATTGAGGAGAGAAGAGAAAAGAAGACAGAGAAGGTTATGCATGCCCTTCTCAATTCACTCTTTGGAAATGTTAAGGTTGGAGAAAATGGGAAGCTGATAATTAACATTGATGGCAATGTTGCAGAACTCAACAAAGAAAGTGGTGAAGTTGAGAGTGAAAATGAAGGCCTTAAAGAAAGAGTGAGAACTGCATTCCGGCGTATTCAAAGTTCTGTGAAGCCAATTCCTCTTTCTGCGCCGTAG
Protein sequence

>MsG0280006332.01.T01

MTYATKAIYKLLLSDYVKVSKVSVDDMLYDEQDINRSMDKIEVSFFIALIFWCYTAGHVLGAAMFMVDIAGVRVLYTGDYSREEDRHLRAAETPQFSPDVCIIESTYGVQHHQPRHTPRKRFTDVIHSTISQGGRVLIPAYALVVHYISFSAHADSAQTSAFLEELNPPNIILVHGAANEMGRLKQKLMTQFADRNTKILTPKNCQSVEMYFNSQKMAKTIGKLAEKTPEVGETVSGLLVKKSRLKQIYESVEPSVDEESGVPTLLVHDRVTVKHESEKCRKIEERREKKTEKVMHALLNSLFGNVKVGENGKLIINIDGNVAELNKESGEVESENEGLKERVRTAFRRIQSSVKPIPLSAP*