Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280006513.01.T01 | XP_003593287.1 | 95 | 60 | 3 | 0 | 1 | 60 | 100 | 159 | 1.11E-28 | 113 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280006513.01.T01 | B0F6S9 | 95.000 | 60 | 3 | 0 | 1 | 60 | 100 | 159 | 5.29e-29 | 113 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0280006512.01 | MsG0280006513.01 | 0.828559 | 8.405817e-55 | 8.384548e-52 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280006513.01.T01 | MTR_2g009770 | 95.000 | 60 | 3 | 0 | 1 | 60 | 100 | 159 | 1.34e-32 | 113 |
| MsG0280006513.01.T01 | MTR_4g093150 | 71.111 | 45 | 13 | 0 | 29 | 73 | 68 | 112 | 6.70e-15 | 66.2 |
| MsG0280006513.01.T01 | MTR_2g009780 | 73.684 | 38 | 10 | 0 | 35 | 72 | 1 | 38 | 1.26e-11 | 54.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 22 sgRNAs with CRISPR-Local
Find 23 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCTAATGTTAAATGTGATTA+TGG | 0.119561 | 2:-2939369 | MsG0280006513.01.T01:CDS |
| CCTGAGGAAATTGGTGAAAA+TGG | 0.306540 | 2:-2939280 | MsG0280006513.01.T01:CDS |
| ATTTAACATTAGATTTCAAT+TGG | 0.342381 | 2:+2939379 | None:intergenic |
| TGATTATGGGTTGGTGGGAT+TGG | 0.353563 | 2:-2939355 | MsG0280006513.01.T01:CDS |
| TGTAATGTCCCTGAGGAAAT+TGG | 0.422831 | 2:-2939289 | MsG0280006513.01.T01:CDS |
| GAAAGATATGGATGGTGGTT+TGG | 0.434138 | 2:-2939328 | MsG0280006513.01.T01:CDS |
| CTAATGTTAAATGTGATTAT+GGG | 0.439770 | 2:-2939368 | MsG0280006513.01.T01:CDS |
| GGGATTGGATGTGAAAGATA+TGG | 0.463932 | 2:-2939340 | MsG0280006513.01.T01:CDS |
| TTTAACATTAGATTTCAATT+GGG | 0.504715 | 2:+2939380 | None:intergenic |
| TGCTTCTAGTGCTGCAGAGG+AGG | 0.524650 | 2:-2939205 | MsG0280006513.01.T01:CDS |
| AAATGTGATTATGGGTTGGT+GGG | 0.536720 | 2:-2939360 | MsG0280006513.01.T01:CDS |
| GAGGCAATGGCAATGATTTG+AGG | 0.549540 | 2:-2939186 | None:intergenic |
| AGCTGCTTCTAGTGCTGCAG+AGG | 0.555002 | 2:-2939208 | MsG0280006513.01.T01:CDS |
| TAGTGCTGCAGAGGAGGCAA+TGG | 0.556888 | 2:-2939199 | MsG0280006513.01.T01:CDS |
| GGAAATTGGTGAAAATGGAG+AGG | 0.578205 | 2:-2939275 | MsG0280006513.01.T01:CDS |
| TAAATGTGATTATGGGTTGG+TGG | 0.605458 | 2:-2939361 | MsG0280006513.01.T01:CDS |
| TGTTAAATGTGATTATGGGT+TGG | 0.606732 | 2:-2939364 | MsG0280006513.01.T01:CDS |
| GGATGGTGGTTTGGAAGATG+AGG | 0.615389 | 2:-2939319 | MsG0280006513.01.T01:CDS |
| GAAATTGGTGAAAATGGAGA+GGG | 0.626252 | 2:-2939274 | MsG0280006513.01.T01:CDS |
| TTGGATGTGAAAGATATGGA+TGG | 0.652894 | 2:-2939336 | MsG0280006513.01.T01:CDS |
| GATGTGAAAGATATGGATGG+TGG | 0.667532 | 2:-2939333 | MsG0280006513.01.T01:CDS |
| TGTTTGATGTAATGTCCCTG+AGG | 0.781479 | 2:-2939296 | MsG0280006513.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATTTAACATTAGATTTCAAT+TGG | + | Chr2:2939199-2939218 | None:intergenic | 15.0% |
| !! | TTTAACATTAGATTTCAATT+GGG | + | Chr2:2939198-2939217 | None:intergenic | 15.0% |
| !! | CTAATGTTAAATGTGATTAT+GGG | - | Chr2:2939207-2939226 | MsG0280006513.01.T01:CDS | 20.0% |
| !! | TCTAATGTTAAATGTGATTA+TGG | - | Chr2:2939206-2939225 | MsG0280006513.01.T01:CDS | 20.0% |
| TGTTAAATGTGATTATGGGT+TGG | - | Chr2:2939211-2939230 | MsG0280006513.01.T01:CDS | 30.0% | |
| GAAATTGGTGAAAATGGAGA+GGG | - | Chr2:2939301-2939320 | MsG0280006513.01.T01:CDS | 35.0% | |
| TTGGATGTGAAAGATATGGA+TGG | - | Chr2:2939239-2939258 | MsG0280006513.01.T01:CDS | 35.0% | |
| ! | CATTTTCACCAATTTCCTCA+GGG | + | Chr2:2939297-2939316 | None:intergenic | 35.0% |
| !! | AAATGTGATTATGGGTTGGT+GGG | - | Chr2:2939215-2939234 | MsG0280006513.01.T01:CDS | 35.0% |
| !! | TAAATGTGATTATGGGTTGG+TGG | - | Chr2:2939214-2939233 | MsG0280006513.01.T01:CDS | 35.0% |
| CCTGAGGAAATTGGTGAAAA+TGG | - | Chr2:2939295-2939314 | MsG0280006513.01.T01:CDS | 40.0% | |
| GATGTGAAAGATATGGATGG+TGG | - | Chr2:2939242-2939261 | MsG0280006513.01.T01:CDS | 40.0% | |
| GGAAATTGGTGAAAATGGAG+AGG | - | Chr2:2939300-2939319 | MsG0280006513.01.T01:CDS | 40.0% | |
| GGGATTGGATGTGAAAGATA+TGG | - | Chr2:2939235-2939254 | MsG0280006513.01.T01:CDS | 40.0% | |
| TGTAATGTCCCTGAGGAAAT+TGG | - | Chr2:2939286-2939305 | MsG0280006513.01.T01:CDS | 40.0% | |
| TGTTTGATGTAATGTCCCTG+AGG | - | Chr2:2939279-2939298 | MsG0280006513.01.T01:CDS | 40.0% | |
| ! | CCATTTTCACCAATTTCCTC+AGG | + | Chr2:2939298-2939317 | None:intergenic | 40.0% |
| ! | GAAAGATATGGATGGTGGTT+TGG | - | Chr2:2939247-2939266 | MsG0280006513.01.T01:CDS | 40.0% |
| !! | TGATTATGGGTTGGTGGGAT+TGG | - | Chr2:2939220-2939239 | MsG0280006513.01.T01:CDS | 45.0% |
| ! | GGATGGTGGTTTGGAAGATG+AGG | - | Chr2:2939256-2939275 | MsG0280006513.01.T01:CDS | 50.0% |
| AGCTGCTTCTAGTGCTGCAG+AGG | - | Chr2:2939367-2939386 | MsG0280006513.01.T01:CDS | 55.0% | |
| TAGTGCTGCAGAGGAGGCAA+TGG | - | Chr2:2939376-2939395 | MsG0280006513.01.T01:CDS | 55.0% | |
| TGCTTCTAGTGCTGCAGAGG+AGG | - | Chr2:2939370-2939389 | MsG0280006513.01.T01:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr2 | gene | 2939188 | 2939409 | 2939188 | ID=MsG0280006513.01;Name=MsG0280006513.01 |
| Chr2 | mRNA | 2939188 | 2939409 | 2939188 | ID=MsG0280006513.01.T01;Parent=MsG0280006513.01;Name=MsG0280006513.01.T01;_AED=0.41;_eAED=0.41;_QI=0|-1|0|1|-1|1|1|0|73 |
| Chr2 | exon | 2939188 | 2939409 | 2939188 | ID=MsG0280006513.01.T01:exon:1618;Parent=MsG0280006513.01.T01 |
| Chr2 | CDS | 2939188 | 2939409 | 2939188 | ID=MsG0280006513.01.T01:cds;Parent=MsG0280006513.01.T01 |
| Gene Sequence |
| Protein sequence |
