AlfalfaGEDB | MsG0280006512.01

M. sativa cultivar ZhongmuNo.1 / MsG0280006512.01

KEGG: -
GO: -
PFAM: PF04576

Nr:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280006512.01.T01	XP_003593287.1	98.765	81	1	0	1	81	192	272	1.84E-47	162

Swissprot:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mis matches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280006512.01.T01	B0F6S9	98.765	81	1	0	1	81	192	272	8.78e-48	162

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network

Gene1	Gene2	correlation coefficient	p_value	FDR
MsG0080048113.01	MsG0280006512.01	0.803811	2.917588e-49	1.480245e-46
MsG0080048404.01	MsG0280006512.01	0.807683	4.483105e-50	2.512975e-47
MsG0180002547.01	MsG0280006512.01	0.800622	1.321952e-48	6.184149e-46
MsG0180004389.01	MsG0280006512.01	0.839919	1.188027e-57	1.665247e-54
MsG0180005800.01	MsG0280006512.01	0.805451	1.326661e-49	7.021117e-47
MsG0280006405.01	MsG0280006512.01	0.800577	1.350518e-48	6.310551e-46
MsG0280006512.01	MsG0280006513.01	0.828559	8.405817e-55	8.384548e-52
MsG0280006512.01	MsG0280007527.01	0.809904	1.502260e-50	8.929780e-48
MsG0280006512.01	MsG0280007955.01	0.813568	2.396823e-51	1.570755e-48
MsG0280006512.01	MsG0280010489.01	0.833153	6.281232e-56	7.172403e-53
MsG0280006512.01	MsG0280010722.01	0.810984	8.782646e-51	5.372461e-48
MsG0280006512.01	MsG0280011101.01	0.804866	1.758346e-49	9.167694e-47
MsG0280006512.01	MsG0280011339.01	0.810092	1.368447e-50	8.174206e-48
MsG0280006512.01	MsG0380014537.01	0.822867	1.882096e-53	1.594695e-50
MsG0280006512.01	MsG0380015555.01	0.834253	3.335461e-56	3.936513e-53
MsG0280006512.01	MsG0480019416.01	0.809605	1.741957e-50	1.027374e-47
MsG0280006512.01	MsG0480020447.01	0.808637	2.808262e-50	1.613982e-47
MsG0280006512.01	MsG0480021452.01	0.811696	6.153403e-51	3.834768e-48
MsG0280006512.01	MsG0480022696.01	0.800850	1.187842e-48	5.589166e-46
MsG0280006512.01	MsG0480023636.01	0.822123	2.802968e-53	2.325036e-50
MsG0280006512.01	MsG0580024197.01	0.821116	4.791132e-53	3.863198e-50
MsG0280006512.01	MsG0580025587.01	0.804512	2.084608e-49	1.076836e-46
MsG0280006512.01	MsG0580026086.01	0.808580	2.887883e-50	1.657331e-47
MsG0280006512.01	MsG0580027925.01	0.800517	1.388468e-48	6.478126e-46
MsG0280006512.01	MsG0780041391.01	0.802326	5.915181e-49	2.889493e-46
MsG0280006512.01	MsG0880045736.01	0.802788	4.750843e-49	2.348147e-46

PPI

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MsG0280006512.01.T01	MTR_2g009770	98.765	81	1	0	1	81	192	272	2.23e-51	162

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 7 sgRNAs with CRISPR-Local

Find 15 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
AGAAACTGAGGGAGTTTATG+AGG	0.393474	2:-2938997	MsG0280006512.01.T01:CDS
GATGAAATTGAGTTAATTGA+AGG	0.529289	2:-2938971	MsG0280006512.01.T01:CDS
GAAACTGAGGGAGTTTATGA+GGG	0.552092	2:-2938996	MsG0280006512.01.T01:CDS
TCAGTTTCGGAGGATGGTGG+AGG	0.564122	2:-2939125	None:intergenic
AAGTGATTGAATCGTTGAGG+TGG	0.581838	2:-2939081	MsG0280006512.01.T01:CDS
ACGAAGTGATTGAATCGTTG+AGG	0.637499	2:-2939084	MsG0280006512.01.T01:CDS
AGTGATTGAATCGTTGAGGT+GGG	0.678874	2:-2939080	MsG0280006512.01.T01:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	GATGAAATTGAGTTAATTGA+AGG	-	Chr2:2939030-2939049	MsG0280006512.01.T01:CDS	25.0%
!!	GATTTTTAAGGAGAAACTGA+GGG	-	Chr2:2938993-2939012	MsG0280006512.01.T01:CDS	30.0%
!	AGAAGAGTTGGGGATTTTTA+AGG	-	Chr2:2938981-2939000	MsG0280006512.01.T01:CDS	35.0%
!!	AGCTTTTTGGAAGAAGAGTT+GGG	-	Chr2:2938970-2938989	MsG0280006512.01.T01:CDS	35.0%
!!	GGATTTTTAAGGAGAAACTG+AGG	-	Chr2:2938992-2939011	MsG0280006512.01.T01:CDS	35.0%
	AAGTGATTGAATCGTTGAGG+TGG	-	Chr2:2938920-2938939	MsG0280006512.01.T01:CDS	40.0%
	ACGAAGTGATTGAATCGTTG+AGG	-	Chr2:2938917-2938936	MsG0280006512.01.T01:CDS	40.0%
	AGAAACTGAGGGAGTTTATG+AGG	-	Chr2:2939004-2939023	MsG0280006512.01.T01:CDS	40.0%
	AGTGATTGAATCGTTGAGGT+GGG	-	Chr2:2938921-2938940	MsG0280006512.01.T01:CDS	40.0%
	GAAACTGAGGGAGTTTATGA+GGG	-	Chr2:2939005-2939024	MsG0280006512.01.T01:CDS	40.0%
!	TGAGTCTCAGAAGAGCTTTT+TGG	-	Chr2:2938957-2938976	MsG0280006512.01.T01:CDS	40.0%
!!	GAGCTTTTTGGAAGAAGAGT+TGG	-	Chr2:2938969-2938988	MsG0280006512.01.T01:CDS	40.0%
!!	GCTTTTTGGAAGAAGAGTTG+GGG	-	Chr2:2938971-2938990	MsG0280006512.01.T01:CDS	40.0%
!!!	TGAAGGTGCTGATTTTGCTA+GGG	-	Chr2:2939047-2939066	MsG0280006512.01.T01:CDS	40.0%
!!!	TTGAAGGTGCTGATTTTGCT+AGG	-	Chr2:2939046-2939065	MsG0280006512.01.T01:CDS	40.0%

Chromosome	Type	Strat	End	Strand	Name
Chr2	gene	2938889	2939134	2938889	ID=MsG0280006512.01;Name=MsG0280006512.01
Chr2	mRNA	2938889	2939134	2938889	ID=MsG0280006512.01.T01;Parent=MsG0280006512.01;Name=MsG0280006512.01.T01;_AED=0.40;_eAED=0.40;_QI=0\|-1\|0\|1\|-1\|1\|1\|0\|81
Chr2	exon	2938889	2939134	2938889	ID=MsG0280006512.01.T01:exon:1617;Parent=MsG0280006512.01.T01
Chr2	CDS	2938889	2939134	2938889	ID=MsG0280006512.01.T01:cds;Parent=MsG0280006512.01.T01

Gene Sequence	>MsG0280006512.01.T01 ATGGTGGAGGAGAGACAAGAGTATGATCACGAAGTGATTGAATCGTTGAGGTGGGATGTTGTGCAGCTTGAGTCTCAGAAGAGCTTTTTGGAAGAAGAGTTGGGGATTTTTAAGGAGAAACTGAGGGAGTTTATGAGGGATGATGAAATTGAGTTAATTGAAGGTGCTGATTTTGCTAGGGAATTCTGTAATTTCTCTGTTGAATATGATCTAGATGATTCTTTAGATGATACTGCATCACACTAA
Protein sequence	>MsG0280006512.01.T01 MVEERQEYDHEVIESLRWDVVQLESQKSFLEEELGIFKEKLREFMRDDEIELIEGADFAREFCNFSVEYDLDDSLDDTASH*