Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280006531.01.T01 | XP_039686156.1 | 91.942 | 484 | 39 | 0 | 14 | 497 | 20 | 503 | 0 | 898 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280006531.01.T01 | A0A396J997 | 91.942 | 484 | 39 | 0 | 14 | 497 | 53 | 536 | 0.0 | 897 |
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0280006530.01 | MsG0280006531.01 | 0.815051 | 1.126817e-51 | 7.686723e-49 |
| MsG0280006531.01 | MsG0280010905.01 | 0.823393 | 1.419329e-53 | 1.220380e-50 |
| MsG0280006531.01 | MsG0780037668.01 | 0.949339 | 1.554020e-107 | 3.064884e-102 |
| MsG0280006531.01 | MsG0880044333.01 | 0.802003 | 6.894880e-49 | 3.340159e-46 |
| MsG0280006531.01 | MsG0880045917.01 | 0.808382 | 3.183477e-50 | 1.817633e-47 |
PPI
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280006531.01.T01 | MTR_2g010250 | 88.683 | 486 | 53 | 1 | 14 | 497 | 20 | 505 | 0.0 | 887 |
| MsG0280006531.01.T01 | MTR_2g010300 | 89.050 | 484 | 39 | 1 | 14 | 497 | 20 | 489 | 0.0 | 861 |
| MsG0280006531.01.T01 | MTR_2g010330 | 86.609 | 463 | 62 | 0 | 35 | 497 | 45 | 507 | 0.0 | 843 |
| MsG0280006531.01.T01 | MTR_2g010280 | 90.496 | 484 | 46 | 0 | 14 | 497 | 20 | 503 | 0.0 | 838 |
| MsG0280006531.01.T01 | MTR_2g010290 | 83.333 | 486 | 63 | 2 | 14 | 497 | 63 | 532 | 0.0 | 822 |
| MsG0280006531.01.T01 | MTR_2g010380 | 84.086 | 465 | 72 | 1 | 35 | 497 | 45 | 509 | 0.0 | 810 |
| MsG0280006531.01.T01 | MTR_2g010320 | 84.091 | 484 | 77 | 0 | 14 | 497 | 20 | 503 | 0.0 | 796 |
| MsG0280006531.01.T01 | MTR_8g098565 | 74.675 | 462 | 112 | 4 | 40 | 497 | 46 | 506 | 0.0 | 675 |
| MsG0280006531.01.T01 | MTR_8g063280 | 59.756 | 492 | 179 | 9 | 14 | 497 | 18 | 498 | 0.0 | 583 |
| MsG0280006531.01.T01 | MTR_5g034900 | 56.237 | 489 | 197 | 5 | 24 | 497 | 31 | 517 | 0.0 | 552 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280006531.01.T01 | AT1G64950 | 58.197 | 488 | 190 | 4 | 19 | 498 | 27 | 508 | 0.0 | 574 |
| MsG0280006531.01.T01 | AT1G64940 | 56.263 | 487 | 199 | 4 | 20 | 498 | 29 | 509 | 0.0 | 571 |
| MsG0280006531.01.T01 | AT2G12190 | 57.971 | 483 | 189 | 4 | 19 | 492 | 27 | 504 | 0.0 | 563 |
| MsG0280006531.01.T01 | AT1G64930 | 53.988 | 489 | 210 | 5 | 19 | 498 | 27 | 509 | 0.0 | 535 |
| MsG0280006531.01.T01 | AT3G03470 | 52.738 | 493 | 214 | 7 | 18 | 498 | 25 | 510 | 3.28e-179 | 513 |
| MsG0280006531.01.T01 | AT1G64900 | 54.641 | 474 | 202 | 5 | 31 | 498 | 38 | 504 | 1.31e-172 | 496 |
| MsG0280006531.01.T01 | AT5G61320 | 55.142 | 457 | 184 | 4 | 38 | 492 | 45 | 482 | 4.49e-169 | 486 |
| MsG0280006531.01.T01 | AT3G10560 | 37.996 | 479 | 280 | 9 | 24 | 497 | 46 | 512 | 2.21e-110 | 337 |
| MsG0280006531.01.T01 | AT5G04660 | 37.339 | 466 | 281 | 7 | 36 | 497 | 53 | 511 | 4.11e-109 | 334 |
| MsG0280006531.01.T01 | AT3G10570 | 37.339 | 466 | 280 | 7 | 36 | 497 | 55 | 512 | 3.73e-108 | 331 |
Find 129 sgRNAs with CRISPR-Local
Find 167 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAAACCCTCCATGCCCGTCA+TGG | 0.568996 | 2:+3180102 | MsG0280006531.01.T01:CDS |
| AAATCAACATGGCCGCCCTC+TGG | 0.446965 | 2:-3181353 | None:intergenic |
| AACAAAATCCTTGTAACTTT+CGG | 0.318882 | 2:-3180597 | None:intergenic |
| AAGAGCGAGGAAGCAAGTTA+AGG | 0.446818 | 2:+3180686 | MsG0280006531.01.T01:CDS |
| AAGATGATGCCGTTTGGAGC+TGG | 0.470239 | 2:+3181242 | MsG0280006531.01.T01:CDS |
| AAGCGGAGCTGATGTTGTGT+TGG | 0.612903 | 2:-3180269 | None:intergenic |
| AATTGGATGTTTGTGATGAT+GGG | 0.510750 | 2:-3180042 | None:intergenic |
| AATTTCTTGCTGGCGGAGAT+GGG | 0.375720 | 2:+3181125 | MsG0280006531.01.T01:CDS |
| ACAAGCCTCCCCTGCACTTC+TGG | 0.187951 | 2:-3180897 | None:intergenic |
| ACACGCGGTGAAAGAGGATG+TGG | 0.582160 | 2:+3181064 | MsG0280006531.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AATTGGATGTTTGTGATGAT+GGG | - | Chr2:3180045-3180064 | None:intergenic | 30.0% | |
| GAAGTTGAGTGAAAATGAAA+TGG | + | Chr2:3180788-3180807 | MsG0280006531.01.T01:CDS | 30.0% | |
| GGAAAAAGATGAATTCACTA+TGG | + | Chr2:3181379-3181398 | MsG0280006531.01.T01:CDS | 30.0% | |
| TAGCCACAAAATATTCCAAA+TGG | - | Chr2:3181304-3181323 | None:intergenic | 30.0% | |
| CAATTGGATGTTTGTGATGA+TGG | - | Chr2:3180046-3180065 | None:intergenic | 35.0% | |
| CCAAATCCTTCTCAGAAATT+CGG | + | Chr2:3180358-3180377 | MsG0280006531.01.T01:CDS | 35.0% | |
| GAAGTTACTTAAGGATCAAG+AGG | + | Chr2:3180644-3180663 | MsG0280006531.01.T01:CDS | 35.0% | |
| GGAGAAATATGAACTTGCAA+TGG | - | Chr2:3181416-3181435 | None:intergenic | 35.0% | |
| TCATCCTTCAAAAACCTTTC+TGG | - | Chr2:3181191-3181210 | None:intergenic | 35.0% | |
| TGATGAAAATGGAGAGAAGA+AGG | + | Chr2:3180947-3180966 | MsG0280006531.01.T01:CDS | 35.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr2 | gene | 3179949 | 3181445 | 3179949 | ID=MsG0280006531.01;Name=MsG0280006531.01 |
| Chr2 | mRNA | 3179949 | 3181445 | 3179949 | ID=MsG0280006531.01.T01;Parent=MsG0280006531.01;Name=MsG0280006531.01.T01;_AED=0.48;_eAED=0.48;_QI=0|-1|0|1|-1|1|1|0|498 |
| Chr2 | exon | 3179949 | 3181445 | 3179949 | ID=MsG0280006531.01.T01:exon:663;Parent=MsG0280006531.01.T01 |
| Chr2 | CDS | 3179949 | 3181445 | 3179949 | ID=MsG0280006531.01.T01:cds;Parent=MsG0280006531.01.T01 |
| Gene Sequence |
>MsG0280006531.01.T01 ATGGTTCATCGTCCTCGTTACTCTTTGCATCATCTTCCTATTATCTCCTCCTTCACAACCACCGTACCCCTCCCTCCCGGCCCTCCACACATCCCCATCATCACAAACATCCAATTGCTCCGAAGATCATTCTACCAACTCGAACCTTTTCTCAAAACCCTCCATGCCCGTCATGGTCCTATCATCACTGTCCATATCGGTTCTCGACCTTCCATTTTCATCTCCGACCATGCTCTGGCTCACCATGTTCTCGTTCAAAACTCAGTCATCTTCTCTGATCGCCCTACAGCTCTTCCTACTTCCAAAATGTTGTCCAGCAACCAACACAACATCAGCTCCGCTTTTTATGGCCCAACATGGCGTACCCTCCGTCGTAACCTTGCTTACGAGATGCTTCATCCATCCAAAACCAAATCCTTCTCAGAAATTCGGAAGTGGGTCTTGCACACTCTCATCAACCGTCTCAGAACTGCATCGGAATCTGCAGATTCTGTTACGGCTGTTTCTCACTTTCGTCGTGCTATGTTTTGTTTACTTGTTTTTATGTGTTTTGGTGAGAGAGTTAACGATGAGAAAATCAGTGATATTGAGCGTGTACAAAGGACAATTCTGTTGAATATTAGCAGATTCAATATACTGAATTTTTGGCCGAAAGTTACAAGGATTTTGTTAGGTAACCGATGGGAGGAGTTTTTGAAGTTACTTAAGGATCAAGAGGATGTTCTGCTTCCGCTGATAAGAGCGAGGAAGCAAGTTAAGGAGAGTAAATTGAACAATGTTAATACAGTTGTTTCGTATGCAGATACTCTTTTAGAGTTGGAGTTTCCCGAGGAGAAACGGAAGTTGAGTGAAAATGAAATGGTTAATCTTTGTTCGGAGTTTTTGAACGCTGGGACGGATACTACTTCCACTGCTTTGCAGTGGATTATGGCGAATTTGGTGAAGTACCCAGAAGTGCAGGGGAGGCTTGTGGAGGAGATTCGGGAGGTGATGGGTGGTGATGAAAATGGAGAGAAGAAGGAAGTGAAAGAGGAAGACTTGCAGAAACTTCGGTATTTGAAGTGTGTGGTTTTGGAAGGGTTGAGGCGTCATCCACCAGGGCATTTTGTCTTGCCACACGCGGTGAAAGAGGATGTGGTTTTGAACGGTTATTTGGTGCCTAAAAATGGGACGGTGAATTTCTTGCTGGCGGAGATGGGGTGGGATCCTCGTGTTTGGGAGGATCCAATGGAGTTTAAGCCAGAAAGGTTTTTGAAGGATGAGACTTTTGATATTACAGGGAGTAAAGAGATAAAGATGATGCCGTTTGGAGCTGGAAGAAGAATTTGTCGCGGACTTAATTTAGCTTTGCTCCATTTGGAATATTTTGTGGCTAATTTGGTTTGGAATTTCGATTGGAAGCTTCCAGAGGGCGGCCATGTTGATTTGTCGGAAAAAGATGAATTCACTATGGTGATGAAAAATCCATTGCAAGTTCATATTTCTCCTAGGCAATAG |
| Protein sequence |
>MsG0280006531.01.T01 MVHRPRYSLHHLPIISSFTTTVPLPPGPPHIPIITNIQLLRRSFYQLEPFLKTLHARHGPIITVHIGSRPSIFISDHALAHHVLVQNSVIFSDRPTALPTSKMLSSNQHNISSAFYGPTWRTLRRNLAYEMLHPSKTKSFSEIRKWVLHTLINRLRTASESADSVTAVSHFRRAMFCLLVFMCFGERVNDEKISDIERVQRTILLNISRFNILNFWPKVTRILLGNRWEEFLKLLKDQEDVLLPLIRARKQVKESKLNNVNTVVSYADTLLELEFPEEKRKLSENEMVNLCSEFLNAGTDTTSTALQWIMANLVKYPEVQGRLVEEIREVMGGDENGEKKEVKEEDLQKLRYLKCVVLEGLRRHPPGHFVLPHAVKEDVVLNGYLVPKNGTVNFLLAEMGWDPRVWEDPMEFKPERFLKDETFDITGSKEIKMMPFGAGRRICRGLNLALLHLEYFVANLVWNFDWKLPEGGHVDLSEKDEFTMVMKNPLQVHISPRQ* |
