Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280006705.01.T01 | XP_003593538.1 | 99.174 | 121 | 1 | 0 | 1 | 121 | 1 | 121 | 2.89E-81 | 251 |
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mis matches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280006705.01.T01 | G7IP27 | 99.174 | 121 | 1 | 0 | 1 | 121 | 1 | 121 | 1.38e-81 | 251 |
| Gene ID | Type | Classification |
|---|
| Gene ID | Type | Classification |
|---|
Co-expression Network
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MsG0180005873.01 | MsG0280006705.01 | 0.867797 | 1.049725e-65 | 3.711537e-62 |
| MsG0280006705.01 | MsG0280009932.01 | 0.802636 | 5.107298e-49 | 2.514431e-46 |
| MsG0280006705.01 | MsG0380015972.01 | 0.822772 | 1.981138e-53 | 1.674105e-50 |
| MsG0280006705.01 | MsG0380017868.01 | 0.852812 | 3.609965e-61 | 7.637463e-58 |
| MsG0280006705.01 | MsG0480021238.01 | 0.813775 | 2.157350e-51 | 1.421733e-48 |
| MsG0280006705.01 | MsG0480023188.01 | 0.821799 | 3.331042e-53 | 2.737791e-50 |
| MsG0280006705.01 | MsG0880046832.01 | 0.812736 | 3.648069e-51 | 2.337822e-48 |
PPI
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MsG0280006705.01.T01 | MTR_2g013220 | 99.174 | 121 | 1 | 0 | 1 | 121 | 1 | 121 | 3.49e-85 | 251 |
| MsG0280006705.01.T01 | MTR_4g088980 | 60.194 | 103 | 35 | 4 | 21 | 121 | 9 | 107 | 7.39e-25 | 95.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 43 sgRNAs with CRISPR-Local
Find 44 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGTGACATCGCCGTCGAAAT+TGG | 0.302399 | 2:+5189876 | None:intergenic |
| AACGATAACGGAAACGGTTA+TGG | 0.352071 | 2:-5189745 | MsG0280006705.01.T01:CDS |
| ATTGGAGTAACCACCGTAAC+CGG | 0.372444 | 2:+5189894 | None:intergenic |
| CTCTCCTTCTCCGTTTGAAT+CGG | 0.398479 | 2:-5189785 | MsG0280006705.01.T01:CDS |
| GAATCGGTTACTGTAGAAAA+CGG | 0.424815 | 2:-5189769 | MsG0280006705.01.T01:CDS |
| GATCAAGACGACACCTTCTC+CGG | 0.433643 | 2:-5189913 | MsG0280006705.01.T01:CDS |
| GACGACACCTTCTCCGGTTA+CGG | 0.443450 | 2:-5189907 | MsG0280006705.01.T01:CDS |
| AATCGGTTACTGTAGAAAAC+GGG | 0.471773 | 2:-5189768 | MsG0280006705.01.T01:CDS |
| CCTTCCTCCACCTGGTGAGA+TGG | 0.476417 | 2:-5189686 | MsG0280006705.01.T01:CDS |
| AGAAGGAGAGTAACCAGGAT+TGG | 0.477559 | 2:+5189798 | None:intergenic |
| GAAGGAGAGTAACCAGGATT+GGG | 0.484433 | 2:+5189799 | None:intergenic |
| GTAGAAAACGGGAACGATAA+CGG | 0.485632 | 2:-5189757 | MsG0280006705.01.T01:CDS |
| GGTCCGATCCTTCCTCCACC+TGG | 0.491267 | 2:-5189694 | MsG0280006705.01.T01:CDS |
| GGATTCAGTGATCCCAATCC+TGG | 0.519749 | 2:-5189811 | MsG0280006705.01.T01:CDS |
| AAGGTTACGCTCTTCGTGAA+TGG | 0.527926 | 2:-5189654 | MsG0280006705.01.T01:CDS |
| TCACTGAATCCGTAGATCTC+CGG | 0.530021 | 2:+5189823 | None:intergenic |
| GTCGTCAGTGAAAGCGTCGA+AGG | 0.533146 | 2:+5189978 | None:intergenic |
| TCTACAGTAACCGATTCAAA+CGG | 0.534276 | 2:+5189775 | None:intergenic |
| GGAGAAGGTGTCGTCTTGAT+CGG | 0.546309 | 2:+5189915 | None:intergenic |
| ACGCTCTTCGTGAATGGCGC+CGG | 0.553734 | 2:-5189648 | MsG0280006705.01.T01:CDS |
| AACGGGAACGATAACGGAAA+CGG | 0.554690 | 2:-5189751 | MsG0280006705.01.T01:CDS |
| CCATCTCACCAGGTGGAGGA+AGG | 0.556654 | 2:+5189686 | None:intergenic |
| GACTCCATCTCACCAGGTGG+AGG | 0.561037 | 2:+5189682 | None:intergenic |
| CGCCGCTGAAACTACTTCAC+CGG | 0.562245 | 2:-5189842 | MsG0280006705.01.T01:CDS |
| ACTACTTCACCGGAGATCTA+CGG | 0.578559 | 2:-5189832 | MsG0280006705.01.T01:CDS |
| GGTGAGATGGAGTCGGAGGA+AGG | 0.580041 | 2:-5189673 | MsG0280006705.01.T01:CDS |
| GGTGGTTACTCCAATTTCGA+CGG | 0.586145 | 2:-5189886 | MsG0280006705.01.T01:CDS |
| GGTGTCGTCTTGATCGGAGG+CGG | 0.596468 | 2:+5189921 | None:intergenic |
| TCACCAGGTGGAGGAAGGAT+CGG | 0.597105 | 2:+5189691 | None:intergenic |
| TCGGAGGCGGCGAAGTTGTG+AGG | 0.600626 | 2:+5189934 | None:intergenic |
| GTAACCACCGTAACCGGAGA+AGG | 0.602987 | 2:+5189900 | None:intergenic |
| TCCTCCGACTCCATCTCACC+AGG | 0.604179 | 2:+5189676 | None:intergenic |
| TCCACCTGGTGAGATGGAGT+CGG | 0.612364 | 2:-5189680 | MsG0280006705.01.T01:CDS |
| ACCTGGTGAGATGGAGTCGG+AGG | 0.613781 | 2:-5189677 | MsG0280006705.01.T01:CDS |
| GACACCTTCTCCGGTTACGG+TGG | 0.615312 | 2:-5189904 | MsG0280006705.01.T01:CDS |
| GAAGGTGTCGTCTTGATCGG+AGG | 0.622038 | 2:+5189918 | None:intergenic |
| CTCCGGTGAAGTAGTTTCAG+CGG | 0.641225 | 2:+5189840 | None:intergenic |
| AACGGAGAAGGAGAGTAACC+AGG | 0.644232 | 2:+5189793 | None:intergenic |
| TCCGACTCCATCTCACCAGG+TGG | 0.644893 | 2:+5189679 | None:intergenic |
| TTCAGCGGCGGAGTGATCGA+CGG | 0.648198 | 2:+5189855 | None:intergenic |
| CGGTGAAGTAGTTTCAGCGG+CGG | 0.659180 | 2:+5189843 | None:intergenic |
| GTAACCGATTCAAACGGAGA+AGG | 0.683900 | 2:+5189781 | None:intergenic |
| AACGGTTATGGTGATGATGA+CGG | 0.705580 | 2:-5189733 | MsG0280006705.01.T01:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| AATCGGTTACTGTAGAAAAC+GGG | - | Chr2:5189865-5189884 | MsG0280006705.01.T01:CDS | 35.0% | |
| GAATCGGTTACTGTAGAAAA+CGG | - | Chr2:5189864-5189883 | MsG0280006705.01.T01:CDS | 35.0% | |
| ! | TCTACAGTAACCGATTCAAA+CGG | + | Chr2:5189861-5189880 | None:intergenic | 35.0% |
| GTAGAAAACGGGAACGATAA+CGG | - | Chr2:5189876-5189895 | MsG0280006705.01.T01:CDS | 40.0% | |
| ! | AACGGTTATGGTGATGATGA+CGG | - | Chr2:5189900-5189919 | MsG0280006705.01.T01:CDS | 40.0% |
| !! | AACGATAACGGAAACGGTTA+TGG | - | Chr2:5189888-5189907 | MsG0280006705.01.T01:CDS | 40.0% |
| !!! | GACGGTGTTTTTGTTTCTGA+TGG | - | Chr2:5189918-5189937 | MsG0280006705.01.T01:CDS | 40.0% |
| AAGGTTACGCTCTTCGTGAA+TGG | - | Chr2:5189979-5189998 | MsG0280006705.01.T01:CDS | 45.0% | |
| ACTACTTCACCGGAGATCTA+CGG | - | Chr2:5189801-5189820 | MsG0280006705.01.T01:CDS | 45.0% | |
| AGAAGGAGAGTAACCAGGAT+TGG | + | Chr2:5189838-5189857 | None:intergenic | 45.0% | |
| ATTGGAGTAACCACCGTAAC+CGG | + | Chr2:5189742-5189761 | None:intergenic | 45.0% | |
| CTCTCCTTCTCCGTTTGAAT+CGG | - | Chr2:5189848-5189867 | MsG0280006705.01.T01:CDS | 45.0% | |
| GAAGGAGAGTAACCAGGATT+GGG | + | Chr2:5189837-5189856 | None:intergenic | 45.0% | |
| GGTGGTTACTCCAATTTCGA+CGG | - | Chr2:5189747-5189766 | MsG0280006705.01.T01:CDS | 45.0% | |
| TCACTGAATCCGTAGATCTC+CGG | + | Chr2:5189813-5189832 | None:intergenic | 45.0% | |
| ! | GTAACCGATTCAAACGGAGA+AGG | + | Chr2:5189855-5189874 | None:intergenic | 45.0% |
| !! | AACGGGAACGATAACGGAAA+CGG | - | Chr2:5189882-5189901 | MsG0280006705.01.T01:CDS | 45.0% |
| AACGGAGAAGGAGAGTAACC+AGG | + | Chr2:5189843-5189862 | None:intergenic | 50.0% | |
| CTCCGGTGAAGTAGTTTCAG+CGG | + | Chr2:5189796-5189815 | None:intergenic | 50.0% | |
| GATCAAGACGACACCTTCTC+CGG | - | Chr2:5189720-5189739 | MsG0280006705.01.T01:CDS | 50.0% | |
| GGATTCAGTGATCCCAATCC+TGG | - | Chr2:5189822-5189841 | MsG0280006705.01.T01:CDS | 50.0% | |
| !! | GGAGAAGGTGTCGTCTTGAT+CGG | + | Chr2:5189721-5189740 | None:intergenic | 50.0% |
| CGCCGCTGAAACTACTTCAC+CGG | - | Chr2:5189791-5189810 | MsG0280006705.01.T01:CDS | 55.0% | |
| CGGTGAAGTAGTTTCAGCGG+CGG | + | Chr2:5189793-5189812 | None:intergenic | 55.0% | |
| GACGACACCTTCTCCGGTTA+CGG | - | Chr2:5189726-5189745 | MsG0280006705.01.T01:CDS | 55.0% | |
| GGTGACATCGCCGTCGAAAT+TGG | + | Chr2:5189760-5189779 | None:intergenic | 55.0% | |
| GTAACCACCGTAACCGGAGA+AGG | + | Chr2:5189736-5189755 | None:intergenic | 55.0% | |
| TCACCAGGTGGAGGAAGGAT+CGG | + | Chr2:5189945-5189964 | None:intergenic | 55.0% | |
| TCCACCTGGTGAGATGGAGT+CGG | - | Chr2:5189953-5189972 | MsG0280006705.01.T01:CDS | 55.0% | |
| !! | GAAGGTGTCGTCTTGATCGG+AGG | + | Chr2:5189718-5189737 | None:intergenic | 55.0% |
| !! | GTCGTCAGTGAAAGCGTCGA+AGG | + | Chr2:5189658-5189677 | None:intergenic | 55.0% |
| ACCTGGTGAGATGGAGTCGG+AGG | - | Chr2:5189956-5189975 | MsG0280006705.01.T01:CDS | 60.0% | |
| ACGCTCTTCGTGAATGGCGC+CGG | - | Chr2:5189985-5190004 | MsG0280006705.01.T01:CDS | 60.0% | |
| CCATCTCACCAGGTGGAGGA+AGG | + | Chr2:5189950-5189969 | None:intergenic | 60.0% | |
| CCTTCCTCCACCTGGTGAGA+TGG | - | Chr2:5189947-5189966 | MsG0280006705.01.T01:CDS | 60.0% | |
| GACACCTTCTCCGGTTACGG+TGG | - | Chr2:5189729-5189748 | MsG0280006705.01.T01:CDS | 60.0% | |
| GACTCCATCTCACCAGGTGG+AGG | + | Chr2:5189954-5189973 | None:intergenic | 60.0% | |
| GGTGAGATGGAGTCGGAGGA+AGG | - | Chr2:5189960-5189979 | MsG0280006705.01.T01:CDS | 60.0% | |
| TCCGACTCCATCTCACCAGG+TGG | + | Chr2:5189957-5189976 | None:intergenic | 60.0% | |
| TCCTCCGACTCCATCTCACC+AGG | + | Chr2:5189960-5189979 | None:intergenic | 60.0% | |
| TTCAGCGGCGGAGTGATCGA+CGG | + | Chr2:5189781-5189800 | None:intergenic | 60.0% | |
| ! | GGTGTCGTCTTGATCGGAGG+CGG | + | Chr2:5189715-5189734 | None:intergenic | 60.0% |
| GGTCCGATCCTTCCTCCACC+TGG | - | Chr2:5189939-5189958 | MsG0280006705.01.T01:CDS | 65.0% | |
| TCGGAGGCGGCGAAGTTGTG+AGG | + | Chr2:5189702-5189721 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| Chr2 | gene | 5189645 | 5190010 | 5189645 | ID=MsG0280006705.01;Name=MsG0280006705.01 |
| Chr2 | mRNA | 5189645 | 5190010 | 5189645 | ID=MsG0280006705.01.T01;Parent=MsG0280006705.01;Name=MsG0280006705.01.T01;_AED=0.50;_eAED=0.50;_QI=0|-1|0|1|-1|1|1|0|121 |
| Chr2 | exon | 5189645 | 5190010 | 5189645 | ID=MsG0280006705.01.T01:exon:36263;Parent=MsG0280006705.01.T01 |
| Chr2 | CDS | 5189645 | 5190010 | 5189645 | ID=MsG0280006705.01.T01:cds;Parent=MsG0280006705.01.T01 |
| Gene Sequence |
| Protein sequence |
